Disease-preventing and root-promoting microbial fertilizer and preparation method thereof

Abstract

The present invention relates to a disease-preventing and root-promoting microbial fertilizer and a preparation method thereof, and belongs to the technical field of microbial fertilizers. The disease-preventing and root-promoting microbial fertilizer of the present invention contains the following components in parts by weight: 15-20 parts of a composite microbial agent, 8-10 parts of a composite hydrolyzed peptide, 15-20 parts of rice husk powder, 40-50 parts of humic acid, 20-30 parts of potassium nitrate, and 1-3 parts of manganese sulfate. The microbial fertilizer of the present invention can effectively promote the growth of plant roots, increase the root surface area and volume, develop plant roots, increase the nutrient absorption area, increase the absorption capacity of nutrients such as water and minerals, promote plant growth, and significantly increase plant disease resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial fertilizers and relates to a disease-preventing and root-promoting microbial fertilizer and a preparation method thereof. Background Art

[0002] Roots are the source of life for crops. The powerful role of the root system on crops: 1. Transmission function. The nutrients absorbed by the roots will be transmitted from bottom to top, and the roots will also receive organic matter transported from the aboveground parts. The absorption of water and nutrients by the root system is well known to us and is also one of the most important functions of the root system. 2. Storage function. During the growth of crops, nutrients will be continuously moved to the roots. If the nutrients produced in the aboveground parts are insufficient, nutrients will be transferred from the roots to maintain their normal growth. These partial functions are obvious in root crops such as carrots and potatoes; this function is also reflected in fruit trees. In autumn, before the fruit trees fall leaves, a large amount of nutrients will be transported to the roots and stems, and stored. When they sprout next year, they will be transported from the roots to the aboveground parts, providing sufficient nutrients for the germination of leaf buds and flower buds. 3. Fixation function, fixation and support: In nature, the root system first stabilizes the plant in the soil and supports the aboveground parts to reduce external influences. It can penetrate deep into the soil, so that the plant is well fixed in the soil to prevent lodging. 4. Transporting function: the nutrients absorbed by the roots will be transmitted from bottom to top, and the roots will also receive organic matter transported from the aboveground parts. This part of the function is a continuation of the previous part. The entire root system participates in it, transporting the nutrients absorbed by the root hairs to the branches, leaves and fruits through the vascular tissue. At the same time, the photosynthetic products of the leaves are also transported to various parts of the root system through the vascular tissue to maintain the growth of the root system. 5. Synthesis and secretion function: the root system can synthesize a variety of essential amino acids, alkaloids, etc. for crop growth, and secrete carbohydrates, amino acids, organic acids and other compounds, which have an impact on the surrounding environment and further promote the absorption of nutrients.

[0003] A strong and developed root system is the basis for crop growth and high yield. Only when the root system grows well can the plant be strong and vigorous, and can high yields and increased income be achieved in the later stage. The root system of crops has always been a topic of concern for many farmers. There are a variety of root-promoting products on the market. In order to achieve good rooting effects, most products on the market contain rooting agents and rooting powders, which are plant growth regulators and promoters. However, although this has a good rooting effect at the time, improper use often leads to premature aging of the root system, imbalance of crop hormones, and the phenomenon of only growing roots but not bearing fruits. Therefore, this hormone rooting agent and rooting powder cannot have a good long-term effect on the root system of crops. Summary of the invention

[0004] The main purpose of the present invention is to provide a microbial fertilizer, which can significantly improve the disease prevention ability of crops and promote the growth of crop roots.

[0005] The present invention adopts the following technical solutions to achieve the above purpose:

[0006] A disease-preventing and root-promoting microbial fertilizer comprises the following components in parts by weight:

[0007] 15-20 parts of composite microbial agents, 8-10 parts of composite hydrolyzed peptides, 15-20 parts of rice husk powder, 40-50 parts of humic acid, 20-30 parts of potassium nitrate, and 1-3 parts of manganese sulfate.

[0008] The composite microbial agent contains Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, and Trichoderma viride, and the total number of effective live bacteria is greater than 2.0×10 10 cfu / g.

[0009] The present invention provides a method for preparing the composite microbial agent, which mainly comprises the following steps:

[0010] Step A, taking pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and organic materials, adjusting the water content to 40-50%, composting for 1.5-2 days, and then flattening and cooling to obtain primary fermentation;

[0011] Step B, mixing the primary fermentation product obtained in step A, Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, Trichoderma viride and pea straw powder, adjusting the water content to 55-60%, adjusting the pH to 6.5-7, fermenting at 25-30° C. for 2-3 days, and drying to obtain a composite microbial agent.

[0012] Preferably, in step A of the above-mentioned method for preparing the composite microbial agent, the weight ratio of pea root powder, sodium alginate and organic material is 1:(0.6-0.8):(0.1-0.3).

[0013] In some embodiments, the organic material is cow dung, pig dung, sheep dung, chicken dung, etc.

[0014] Preferably, in step B of the above-mentioned composite microbial agent preparation method, the weight ratio of the primary fermentation product, Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, Trichoderma viride, and pea straw powder is 1:(0.015-0.018):(0.01-0.012):(0.01-0.02):(0.006-0.008):(0.3-0.5).

[0015] In certain embodiments, the effective viable count of Bacillus subtilis is 20 billion / g, the effective viable count of Bacillus mucilaginosus is 20 billion / g, the effective viable count of Bacillus laterosporus is 10 billion / g, and the effective viable count of Trichoderma viride is 5 billion / g.

[0016] In certain embodiments, the effective viable count of the composite microbial agent is greater than 2.0×10 10 cfu / g.

[0017] The present invention provides a method for preparing the composite hydrolyzed peptide, which mainly comprises the following steps:

[0018] Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare homogenate, petroleum ether is added, and the mixture is shaken for 0.5-1h, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing tannase, pancreatin, and pectinase is added to the seabuckthorn solution, and the pH is adjusted to 6-6.5, and enzymolysis is performed at 30-40° C. for 10-12h, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 8-10h, and a precipitate is obtained, which is seabuckthorn peptide;

[0019] Step 2, the seabuckthorn peptide and egg white protein obtained in step 1 are mixed with water, the pH is adjusted to 7-7.5, glutamine aminotransferase and lumbrokinase are added, and enzymatic hydrolysis is carried out at 35-40° C. for 4-6 hours, and heat treatment is carried out at 80-85° C. for 20-30 minutes after enzymatic hydrolysis, and drying is carried out to obtain a composite hydrolyzed peptide.

[0020] Preferably, in step 1 of the above-mentioned method for preparing composite hydrolyzed peptides, the volume ratio of seabuckthorn solution to composite enzyme aqueous solution is 1:(0.8-1).

[0021] Preferably, in step 1 of the composite hydrolyzed peptide preparation method, the composite enzyme aqueous solution contains 3-5 g / L tannase, 22-25 g / L pancreatin, and 15-18 g / L pectinase.

[0022] In certain embodiments, the aqueous solution of the complex enzyme contains 200,000 U / g of tannase, 4,000 U / g of pancreatic enzyme, and 30,000 U / g of pectinase.

[0023] Preferably, in step 2 of the above-mentioned composite hydrolyzed peptide preparation method, the mass volume ratio of seabuckthorn peptide, ovalbumin and water is 1:(0.1-0.15):(10-12) in kg / kg / L.

[0024] Preferably, in step 2 of the composite hydrolyzed peptide preparation method, the amount of glutamine transaminase added is 0.3-0.5 g / L and the amount of lumbrokinase added is 0.01-0.012 g / L.

[0025] In certain embodiments, in step 2 of the composite hydrolyzed peptide preparation method, the enzyme activity of glutamine transaminase is 50,000 U / g, and the enzyme activity of lumbrokinase is 15,000 U / mg.

[0026] The present invention provides a method for preparing the disease-preventing and root-promoting microbial fertilizer, comprising the following steps:

[0027] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0028] Step b, mixing the composite microbial agent, composite hydrolyzed peptide, rice husk powder, humic acid, potassium nitrate and manganese sulfate to obtain a microbial fertilizer.

[0029] The present invention provides a specific use of the microbial fertilizer, that is, the microbial fertilizer can promote the growth and development of crop roots and improve the disease prevention ability of crops.

[0030] The present invention has the following beneficial effects:

[0031] 1. The microorganisms used in the microbial fertilizer of the present invention are composite microorganisms, which use the residual endophytic fungi in pea roots, and through composting and fermentation, make them proliferate to a certain extent and increase the types of microorganisms, and then ferment with a variety of Bacillus and green Trichoderma to obtain dominant flora. The presence of microorganisms such as endophytic fungi can live in or inside the roots of plants, and at the same time as using their substances to grow and reproduce, they can also provide the roots of plants with nutrients, enzymes and other components required for growth, so that the roots of plants can obtain sufficient nutrition and promote their growth and development.

[0032] 2. The present invention adopts a composite hydrolyzed peptide, which firstly destroys the cell wall structure of seabuckthorn fruit by enzymatic hydrolysis, so that the components in the cell are dissolved to obtain the protein component, and then ovalbumin is added to further decompose the protein component into small molecular peptides of different degrees through the action of glutamine aminotransferase and lumbrokinase, which is beneficial to the absorption and utilization of plant roots and promotes their growth and development.

[0033] 3. The use of the microbial fertilizer of the present invention can effectively promote the growth of plant roots, increase their root surface area and volume, develop plant roots, increase their nutrient absorption area, increase their absorption capacity for nutrients such as water and minerals, promote plant growth, and significantly increase plant disease resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 : The root system of cucumber seedlings in test 1, wherein A is the root of cucumber seedlings irrigated with bacterial fertilizer in Example 1, and B is the root of cucumber seedlings irrigated with rooting powder;

[0035] Figure 2 : The root system of garlic in the second test, wherein A is the garlic applied with the bacterial fertilizer of comparative example 3, and B is the garlic applied with the bacterial fertilizer of example 1;

[0036] Figure 3: The root growth of the cutting rose branches in the third test, wherein A is the rose branches irrigated with the bacterial fertilizer of Example 1, B is the rose branches irrigated with the rooting powder, C is the rose branches irrigated with the bacterial fertilizer of Comparative Example 1, D is the rose branches irrigated with the bacterial fertilizer of Comparative Example 2, and E is the rose branches irrigated with clear water. DETAILED DESCRIPTION

[0037] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications to the present invention by those skilled in the art all fall within the scope of protection of the claims of this application.

[0038] Example 1

[0039] Preparation of composite microbial inoculants:

[0040] Step A, taking discarded pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and cow dung in a weight ratio of 1:0.8:0.1, adjusting the water content to 40%, composting for 1.5 days, and then flattening and cooling to obtain a primary fermentation product;

[0041] Step B, the obtained primary fermentation product, Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), and pea straw powder are mixed in a weight ratio of 1:0.018:0.012:0.01:0.008:0.3, the water content is adjusted to 60%, the pH is adjusted to 7, fermented at 30°C for 2 days, and dried to obtain a composite microbial agent. The obtained composite microbial agent is tested, and the total effective live bacteria count is 5.8×10 10 cfu / g.

[0042] Preparation of complex hydrolyzed peptides:

[0043] Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare a homogenate, petroleum ether is added, and the mixture is shaken for 0.5-1h, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing 5g / L tannin (enzyme activity is 200,000 U / g), 22g / L pancreatin (enzyme activity is 4000U / g), and 18g / L pectinase (enzyme activity is 30,000U / g) is added to the seabuckthorn solution in a volume ratio of 1:1, the pH is adjusted to 6, and enzymolysis is performed at 40°C for 10h, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 10h, and centrifuged to obtain a precipitate, which is seabuckthorn peptide;

[0044] Step 2, the sea buckthorn peptide obtained in step 1, ovalbumin, and water are mixed in a mass-volume ratio of 1:0.15:10, the pH is adjusted to 7.5, 0.5 g / L of glutamine transaminase (enzyme activity is 50,000 U / g) and 0.01 g / L of lumbrokinase (enzyme activity is 15,000 U / mg) are added, and enzymolysis is performed at 40°C for 4 hours. After enzymolysis, heat treatment is performed at 85°C for 20 minutes, and drying is performed to obtain a composite hydrolyzed peptide.

[0045] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0046] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0047] Step b, mixing 20 parts of composite microbial agent, 8 parts of composite hydrolyzed peptide, 20 parts of rice husk powder, 40 parts of humic acid, 30 parts of potassium nitrate and 1 part of manganese sulfate to obtain microbial fertilizer.

[0048] Example 2

[0049] Preparation of composite microbial inoculants:

[0050] Step A, taking discarded pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and chicken manure in a weight ratio of 1:0.6:0.3, adjusting the water content to 50%, composting for 2 days, and then flattening and cooling to obtain primary fermentation;

[0051] Step B, the obtained primary fermentation product, Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), and pea straw powder are mixed in a weight ratio of 1:0.015:0.01:0.02:0.006:0.5, the water content is adjusted to 55%, the pH is adjusted to 6.5, fermented at 25°C for 3 days, and dried to obtain a composite microbial agent. The obtained composite microbial agent is tested, and the total effective live bacteria count is 7.3×10 10 cfu / g.

[0052] Preparation of complex hydrolyzed peptides:

[0053] Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare a homogenate, petroleum ether is added, and the mixture is shaken for 0.5-1h, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing 3g / L tannin (enzyme activity is 200,000 U / g), 25g / L pancreatin (enzyme activity is 4000U / g), and 15g / L pectinase (enzyme activity is 30,000U / g) is added to the seabuckthorn solution at a volume ratio of 1:0.8, the pH is adjusted to 6.5, and enzymolysis is performed at 30°C for 12h, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 8h, and centrifuged to obtain a precipitate, which is seabuckthorn peptide;

[0054] Step 2, the sea buckthorn peptide obtained in step 1, ovalbumin, and water are mixed in a mass-volume ratio of 1:0.1:12, the pH is adjusted to 7, 0.3 g / L of glutamine transaminase (enzyme activity is 50,000 U / g) and 0.012 g / L of lumbrokinase (enzyme activity is 15,000 U / mg) are added, and enzymatic hydrolysis is carried out at 35°C for 6 hours. After enzymatic hydrolysis, heat treatment is carried out at 80°C for 30 minutes, and drying is carried out to obtain a composite hydrolyzed peptide.

[0055] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0056] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0057] Step b, mixing 15 parts of composite microbial agent, 10 parts of composite hydrolyzed peptide, 15 parts of rice husk powder, 50 parts of humic acid, 20 parts of potassium nitrate and 3 parts of manganese sulfate to obtain microbial fertilizer.

[0058] Example 3

[0059] Preparation of composite microbial inoculants:

[0060] Step A, taking discarded pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and sheep manure in a weight ratio of 1:0.7:0.2, adjusting the water content to 45%, composting for 2 days, and then flattening and cooling to obtain primary fermentation;

[0061] Step B, the obtained primary fermentation product, Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), and pea straw powder are mixed in a weight ratio of 1:0.016:0.011:0.015:0.007:0.4, the water content is adjusted to 60%, the pH is adjusted to 6.5, and the mixture is fermented at 30°C for 2 days and dried to obtain a composite microbial agent. The obtained composite microbial agent is tested, and the total effective live bacteria count is 2.6×1010 cfu / g.

[0062] Preparation of complex hydrolyzed peptides:

[0063] Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare a homogenate, petroleum ether is added, and the mixture is shaken for 1 hour, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing 4 g / L tannin (enzyme activity is 200,000 U / g), 23 g / L pancreatin (enzyme activity is 4000 U / g), and 16 g / L pectinase (enzyme activity is 30,000 U / g) is added to the seabuckthorn solution at a volume ratio of 1:0.9, the pH is adjusted to 6.5, and enzymolysis is performed at 40°C for 12 hours, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 10 hours, and centrifuged to obtain a precipitate, which is seabuckthorn peptide;

[0064] Step 2, the sea buckthorn peptide obtained in step 1, ovalbumin, and water are mixed in a mass-volume ratio of 1:0.13:11, the pH is adjusted to 7, 0.4 g / L of glutamine transaminase (enzyme activity is 50,000 U / g) and 0.011 g / L of lumbrokinase (enzyme activity is 15,000 U / mg) are added, and enzymolysis is performed at 40°C for 6 hours. After enzymolysis, heat treatment is performed at 80°C for 30 minutes, and drying is performed to obtain a composite hydrolyzed peptide.

[0065] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0066] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0067] Step b, mixing 18 parts of composite microbial agent, 9 parts of composite hydrolyzed peptide, 18 parts of rice husk powder, 45 parts of humic acid, 25 parts of potassium nitrate and 2 parts of manganese sulfate to obtain microbial fertilizer.

[0068] Comparative Example 1

[0069] Preparation of composite microbial inoculants:

[0070] Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), pea straw powder, and sheep manure were mixed in a weight ratio of 0.016:0.011:0.015:0.007:0.4:0.2, the water content was adjusted to 60%, the pH was adjusted to 6.5, and the mixture was fermented at 30°C for 2 days and dried to obtain a composite microbial agent. The obtained composite microbial agent was tested and the total effective live bacteria count was 4.9×10 10 cfu / g.

[0071] Preparation of complex hydrolyzed peptides:

[0072] Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare a homogenate, petroleum ether is added, and the mixture is shaken for 1 hour, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing 4 g / L tannin (enzyme activity is 200,000 U / g), 23 g / L pancreatin (enzyme activity is 4000 U / g), and 16 g / L pectinase (enzyme activity is 30,000 U / g) is added to the seabuckthorn solution at a volume ratio of 1:0.9, the pH is adjusted to 6.5, and enzymolysis is performed at 40°C for 12 hours, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 10 hours, and centrifuged to obtain a precipitate, which is seabuckthorn peptide;

[0073] Step 2, the seabuckthorn peptide obtained in step 1 and water are mixed in a mass-volume ratio of 1:11, the pH is adjusted to 7, 0.4 g / L of glutamine transaminase (enzyme activity is 50,000 U / g) is added, enzymolysis is carried out at 40° C. for 6 hours, and heat treatment is carried out at 80° C. for 30 minutes after enzymolysis, and drying is carried out to obtain a composite hydrolyzed peptide.

[0074] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0075] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0076] Step b, mixing 18 parts of composite microbial agent, 9 parts of composite hydrolyzed peptide, 18 parts of rice husk powder, 45 parts of humic acid, 25 parts of potassium nitrate and 2 parts of manganese sulfate to obtain microbial fertilizer.

[0077] Comparative Example 2

[0078] Preparation of composite microbial inoculants:

[0079] Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), pea root powder, and sodium alginate were mixed in a weight ratio of 0.016:0.011:0.015:0.007:0.4:1:0.7, and the water content was adjusted to 60%, the pH was adjusted to 6.5, and the mixture was fermented at 30°C for 2 days and dried to obtain a composite microbial agent. The obtained composite microbial agent was tested and the total effective live bacteria count was 2.6×10 10 cfu / g.

[0080] Preparation of seabuckthorn peptides:

[0081] The seabuckthorn fruit is crushed and water is added to prepare a homogenate, petroleum ether is added, and the mixture is shaken for 1 hour, and then the mixture is allowed to stand for stratification, and the lower layer of the solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing 4 g / L tannin (enzyme activity is 200,000 U / g), 23 g / L pancreatin (enzyme activity is 4000 U / g), and 16 g / L pectinase (enzyme activity is 30,000 U / g) is added to the seabuckthorn solution at a volume ratio of 1:0.9, the pH is adjusted to 6.5, and the mixture is enzymolyzed at 40°C for 12 hours to separate the supernatant, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 10 hours, and centrifuged to obtain a precipitate, which is the seabuckthorn peptide;

[0082] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0083] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0084] Step b, mixing 18 parts of composite microbial agent, 9 parts of seabuckthorn peptide, 18 parts of rice husk powder, 45 parts of humic acid, 25 parts of potassium nitrate and 2 parts of manganese sulfate to obtain microbial fertilizer.

[0085] Comparative Example 3

[0086] Preparation of composite microbial inoculants:

[0087] Step A, taking discarded pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and sheep manure in a weight ratio of 1:0.7:0.2, adjusting the water content to 45%, composting for 2 days, and then flattening and cooling to obtain primary fermentation;

[0088] Step B, the obtained primary fermentation product, Bacillus subtilis (effective live bacteria count of 20 billion / g), Bacillus mucilaginosus (effective live bacteria count of 20 billion / g), Bacillus laterosporus (effective live bacteria count of 10 billion / g), Trichoderma viride (effective live bacteria count of 5 billion / g), and pea straw powder are mixed in a weight ratio of 1:0.016:0.011:0.015:0.007:0.4, the water content is adjusted to 60%, the pH is adjusted to 6.5, and the mixture is fermented at 30°C for 2 days and dried to obtain a composite microbial agent. The obtained composite microbial agent is tested, and the total effective live bacteria count is 2.6×10 10 cfu / g.

[0089] Preparation of disease-preventing and root-promoting microbial fertilizer:

[0090] Step a, preparing the composite microbial agent and composite hydrolyzed peptide according to the above method;

[0091] Step b, mixing 18 parts of composite microbial agent, 18 parts of rice husk powder, 45 parts of humic acid, 25 parts of potassium nitrate and 2 parts of manganese sulfate to obtain microbial fertilizer.

[0092] Experiment 1: Effect of the microbial agent of the present invention on the root development of cucumber seedlings

[0093] 1.1 Test materials and methods

[0094] The cucumber variety used for the test was "Xinyujinyan No. 4", produced by Hebei Qingfeng Agricultural Technology Co., Ltd. This test was conducted in the test greenhouse of the company's scientific research center.

[0095] Before sowing, cucumber seeds were treated according to conventional operations, specifically: soaked in 75% ethanol by volume for 30 seconds, sterilized with 5% sodium hypochlorite by volume for 12 minutes, rinsed with sterile water and soaked for 12 hours, placed in a constant temperature box at 28°C, and planted in a 32-grid seedling tray after 12 hours. Ten days after the cucumber seedlings were planted, the bacterial fertilizers of Example 1-3 of the present invention (0.2 g / L), the bacterial fertilizers of Comparative Example 1-3 (0.2 g / L), commercially available rooting powder (0.15 g / L), and clean water were used for root irrigation treatment, 10 mL / time / plant, once every 5 days, for a total of 5 root irrigations, and the seedlings were measured 1 day after the last root irrigation.

[0096] Ten cucumber seedlings were randomly selected, and the stem diameter at 5 cm above the ground was measured with a vernier caliper. The roots were then cut off, rinsed, and a panoramic scan of the root system was performed to obtain the total root length, root surface area, and root volume.

[0097] Ten cucumber seedlings were randomly selected, and the fresh weight of the aboveground plants and underground roots of the cucumber seedlings was weighed, then dried to a constant weight and the dry weight was weighed to calculate the root-to-crown ratio.

[0098] 1.2 Results and Analysis

[0099] As can be seen from the results in Table 1, the cucumber seedlings grown with the microbial fertilizers obtained in Examples 1-3 of the present invention have better root growth and development, and their underground fresh weight, root-crown ratio, total root length, root surface area, and root volume are significantly higher. The stems are relatively thick, and the cucumber seedlings are thick and of better quality.

[0100] Table 1 Comparison of root conditions of cucumber seedlings

[0101]

[0102] Experiment 2: Effect of the microbial agent of the present invention on the growth of garlic roots

[0103] 2.1 Experimental materials and methods

[0104] The purple garlic from Jinxiang, Shandong was used in this test. The test site was the company's farmland for scientific research and was sown in spring. Before sowing, the bacterial fertilizers of Example 1-3 (2kg / mu) and the bacterial fertilizers of Comparative Example 1-3 (2kg / mu) were added to the base fertilizer and evenly spread on the surface, and turned over and mixed with a rotary plow to a depth of about 5cm in the soil layer. The planting spacing was 15cm, and only the plots with base fertilizer added were used as the control group. Other field management measures were the same. At the early stage of bulbil expansion, 20 garlic plants were randomly selected from each plot to measure the longest root length and root weight.

[0105] 2.2 Results and Analysis

[0106] From the results in Table 2, it can be seen that the garlic using the microbial fertilizer obtained in Examples 1-3 has a more developed root system, and the longest root length and root weight are significantly higher than the garlic using Comparative Examples 1-3 or base fertilizer.

[0107] Table 2 Comparison of garlic root growth

[0108] deal with Longest root length (cm) Root weight (g) Embodiment 1 bacterial fertilizer 12.6 5.83 Embodiment 2 bacterial fertilizer 11.9 5.64 Embodiment 3 bacterial fertilizer 13.4 6.03 Comparative Example 1 Bacterial Fertilizer 9.03 4.98 Comparative Example 2 Bacterial Fertilizer 8.29 4.61 Comparative Example 3 Bacterial Fertilizer 8.57 5.07 Base fertilizer only 7.51 3.65

[0109] Experiment 3: Effect of the microbial agent of the present invention on the root growth of rose cuttings

[0110] 3.1 Test materials and methods

[0111] The rose variety selected in this test is vine "red dragon sand gem", which is carried out in the greenhouse of our company's scientific research center. Select the semi-lignified branches that grow robust and pest-free as cuttings, the cuttings are about 12cm long, the lower end is pruned to 45 ° of oblique cuts, the upper end is flat, and 1-2 leaflets are retained on the top. After the cuttings are pruned, they are cleaned with clear water, and nutrient solution is used to soak for 1h. After soaking, they are cut in plastic pots. After 5 days of cutting, the present invention embodiment 1-3 bacterial fertilizer (0.2g / L), comparative example 1-3 bacterial fertilizer (0.2g / L), commercially available rooting powder (0.15g / L), clear water are used to irrigate the roots, and arch sheds are built to shade, and subsequent processing is carried out according to a conventional method. Each method processes 50 cuttings, and after 50 days of cutting, 20 plants are randomly selected to measure the number of roots and the longest root length.

[0112] 3.2 Results and Analysis

[0113] From the results in Table 3, it can be seen that the number of roots of rose cuttings using the microbial fertilizer of the present invention is significantly higher, the longest root length is longer, and the rooting of cuttings can be significantly promoted, thereby improving the survival rate of cutting propagated plants.

[0114] Table 3 Comparison of root growth of rose cuttings

[0115]

[0116]

[0117] Experiment 4: Effect of the microbial agent of the present invention on the growth of rice roots

[0118] 4.1 Experimental materials and methods

[0119] This field test was conducted in our company's rice test field. The test field was divided into an area of ​​about 4m long and 3m wide, with partitions set in the area and protective rows set between the areas. The bacterial fertilizers of Example 1-3 (2kg / mu) and the bacterial fertilizers of Comparative Example 1-3 (2kg / mu) were added to the base fertilizer and evenly spread on the surface, mixed with the soil with a rotary plow, and the area where only the base fertilizer was added was used as the control group. After transplanting, conventional field management measures were followed. Root samples of 10 rice plants were randomly collected at the tillering stage, heading stage, and filling stage of rice, and the rice root growth indicators were measured.

[0120] 4.2 Results and Analysis

[0121] From the results in Table 4, it can be seen that the root system of the rice grown using the microbial fertilizers obtained in Examples 1-3 is well developed, and the total root length and root surface area at the tillering stage and the heading stage are significantly higher than those of the rice grown using the microbial fertilizers obtained in Comparative Examples 1-3 or using only basal fertilizer.

[0122] Table 4 Comparison of rice root growth

[0123]

Claims

1. A disease-preventing and root-promoting microbial fertilizer, characterized in that: Contains the following components in parts by weight: 15-20 parts of composite microbial agent, 8-10 parts of composite hydrolyzed peptide, 15-20 parts of rice husk powder, 40-50 parts of humic acid, 20-30 parts of potassium nitrate, 1-3 parts of manganese sulfate; in, The composite microbial agent contains Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, and Trichoderma viride, and the total number of effective live bacteria is greater than 2.0×10 10 cfu / g; The preparation method of the composite microbial agent comprises the following steps: Step A, taking pea plants with roots, separating pea straws and pea roots, and crushing and sieving them respectively to obtain pea straw powder and pea root powder; mixing pea root powder, sodium alginate and organic materials, adjusting the water content to 40-50%, composting for 1.5-2 days, and then flattening and cooling to obtain primary fermentation; Step B, mixing the primary fermentation product obtained in step A, Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, Trichoderma viride and pea straw powder, adjusting the water content to 55-60%, adjusting the pH to 6.5-7, fermenting at 25-30° C. for 2-3 days, and drying to obtain a composite microbial agent; The preparation method of the composite hydrolyzed peptide comprises the following steps: Step 1, preparation of seabuckthorn peptide: seabuckthorn fruit is crushed and water is added to prepare homogenate, petroleum ether is added, and the mixture is shaken for 0.5-1h, and then the mixture is allowed to stand for stratification, and the lower layer of solution is taken, and the residual petroleum ether is removed to obtain a seabuckthorn solution; a composite enzyme aqueous solution containing tannase, pancreatin, and pectinase is added to the seabuckthorn solution, and the pH is adjusted to 6-6.5, and enzymolysis is performed at 30-40° C. for 10-12h, and a supernatant is obtained by separation, and an ethanol aqueous solution with a volume fraction of 80% is added to the supernatant, and the mixture is allowed to stand for 8-10h, and a precipitate is obtained, which is seabuckthorn peptide; Step 2, the seabuckthorn peptide and egg white protein obtained in step 1 are mixed with water, the pH is adjusted to 7-7.5, glutamine aminotransferase and lumbrokinase are added, and enzymatic hydrolysis is carried out at 35-40° C. for 4-6 hours, and heat treatment is carried out at 80-85° C. for 20-30 minutes after enzymatic hydrolysis, and drying is carried out to obtain a composite hydrolyzed peptide.

2. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: The weight ratio of pea root powder, sodium alginate and organic material in step A of the composite microbial agent preparation method is 1:(0.6-0.8):(0.1-0.3).

3. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: The organic material in step A of the composite microbial agent preparation method is one or more of cow dung, pig dung, sheep dung and chicken dung.

4. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: The weight ratio of the primary fermentation product, Bacillus subtilis, Bacillus mucilaginosus, Bacillus laterosporus, Trichoderma viride and pea straw powder in step B of the composite microbial agent preparation method is 1:(0.015-0.018):(0.01-0.012):(0.01-0.02):(0.006-0.008):(0.3-0.5); wherein the effective live bacteria count of Bacillus subtilis is 20 billion / g, the effective live bacteria count of Bacillus mucilaginosus is 20 billion / g, the effective live bacteria count of Bacillus laterosporus is 10 billion / g, and the effective live bacteria count of Trichoderma viride is 5 billion / g.

5. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: In step 1 of the composite hydrolyzed peptide preparation method, the volume ratio of the seabuckthorn solution to the composite enzyme aqueous solution is 1:(0.8-1).

6. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: The composite hydrolyzed peptide preparation method comprises, in step 1, 3-5 g / L of tannase, 22-25 g / L of pancreatin, and 15-18 g / L of pectinase in the composite enzyme aqueous solution; wherein the enzyme activity of tannase is 200,000 U / g, the enzyme activity of pancreatin is 4,000 U / g, and the enzyme activity of pectinase is 30,000 U / g.

7. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: The mass volume ratio of seabuckthorn peptide, ovalbumin and water in step 2 of the composite hydrolyzed peptide preparation method is 1:(0.1-0.15):(10-12) in kg / kg / L.

8. The disease-preventing and root-promoting microbial fertilizer according to claim 1, characterized in that: In step 2 of the composite hydrolyzed peptide preparation method, the added amount of glutamine transaminase is 0.3-0.5 g / L, and the added amount of lumbrokinase is 0.01-0.012 g / L; wherein, the enzyme activity of glutamine transaminase is 50,000 U / g, and the enzyme activity of lumbrokinase is 15,000 U / mg.

9. The disease-preventing and root-promoting microbial fertilizer according to any one of claims 1 to 8, characterized in that: The preparation method of the disease-preventing and root-promoting microbial fertilizer comprises the following steps: Step a, preparing composite microbial agents and composite hydrolyzed peptides; Step b, mixing the composite microbial agent, composite hydrolyzed peptide, rice husk powder, humic acid, potassium nitrate and manganese sulfate to obtain a microbial fertilizer.

10. Use of the disease-preventing and root-promoting microbial fertilizer according to any one of claims 1 to 9 in promoting the growth and development of crop root systems and improving the disease resistance of crops.

Images