A monoclonal antibody based on H5N8 subtype avian influenza virus NA and its application

By developing monoclonal antibodies based on the NA of the H5N8 subtype avian influenza virus and establishing a blocking ELISA method, the problems of the existing detection methods being cumbersome and time-consuming were solved, and rapid and specific detection of the NA8 subtype avian influenza virus was achieved.

CN118834293BActive Publication Date: 2025-09-19YANGZHOU UNIV
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Patent Information

Application Number
CN202411070755.9
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-08-06
Publication Date
2025-09-19
Estimated Expiration
2044-08-06

AI Technical Summary

Technical Problem

Existing AIV detection methods are cumbersome, time-consuming, and not suitable for testing large numbers of serum samples. They are also difficult to quickly and easily distinguish NA subtypes.

Method used

Develop a monoclonal antibody based on the NA of the H5N8 subtype avian influenza virus, establish a blocking ELISA method, and use this antibody for rapid and specific detection.

Benefits of technology

It achieves rapid, simple and efficient detection of NA8 subtype avian influenza virus with good specificity, sensitivity and repeatability, and is suitable for rapid diagnosis of clinical serum samples.

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Abstract

The present invention belongs to the biological field and relates to a monoclonal antibody based on the NA of the H5N8 subtype avian influenza virus and its application. The monoclonal antibody has good reactivity with MDCK cells infected with the NA8 subtype AIV, good broad spectrum, and good specificity, sensitivity, repeatability and accuracy. It can be used for rapid detection of antibodies specific to the NA8 subtype avian influenza.
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