A cosmetic composition comprising blue copper peptide

Abstract

The present application provides a cosmetic composition comprising blue copper peptide and hyaluronic acid or a salt thereof, wherein the hyaluronic acid or a salt thereof is greater than 5 kDa and less than or equal to 900 kDa. The cosmetic composition provided by the present application can overcome the influence of hyaluronic acid substances on the stability of blue copper peptide, thereby obtaining a stable cosmetic composition containing hyaluronic acid substances and blue copper peptide.

Description

[0001] This case is a divisional application of the application filed on September 27, 2022, entitled "A Cosmetic Composition Containing Copper Peptide", with application number 202211180557.9. Technical Field

[0002] This application belongs to the field of cosmetic technology, and specifically relates to a cosmetic composition containing copper peptides. Background Technology

[0003] Blue copper peptide is a complex of glycyl histidine tripeptide (GHK) and copper. GHK is usually used as a complex with divalent copper ions to exert anti-aging, wound healing and antioxidant effects, and it is widely used in skin care products.

[0004] However, when copper peptides are used in skincare products, instability sometimes occurs. Therefore, improving the stability of copper peptides remains a pressing issue in the cosmetics industry. Summary of the Invention

[0005] The technical problem that this application aims to solve is:

[0006] The inventors of this application discovered that the copper peptide exhibited instability when compounding it with hyaluronic acid-like substances. They speculated that this instability might be due to the presence of numerous carboxyl groups in the hyaluronic acid molecule, which have a strong affinity for Cu. 2+ The complexing ability is limited. Therefore, when hyaluronic acid and copper peptides are mixed, the interaction and complexation of copper ions can lead to instability in the composition and reduce the efficacy of the copper peptides. To address this issue, this application provides a stable cosmetic composition containing hyaluronic acid and copper peptides.

[0007] Technical solutions for solving technical problems:

[0008] 1. A cosmetic composition comprising a copper peptide and a hyaluronic acid substance, wherein the hyaluronic acid substance has a molecular weight greater than 5 kDa and less than or equal to 900 kDa.

[0009] 2. The composition according to claim 1, wherein the molecular weight of the hyaluronic acid is 30kDa-900kDa.

[0010] 3. The composition according to item 1 or 2, wherein the mass ratio of the copper peptide to the hyaluronic acid is 1:1 to 1:9.

[0011] 4. The composition according to any one of items 1-3, wherein the content of the hyaluronic acid or its salt in the composition is greater than 0 wt% and less than or equal to 10 wt%.

[0012] 5. The composition according to any one of items 1-4, wherein the content of the hyaluronic acid or its salt in the composition is 0.3wt%-3wt%.

[0013] 6. The composition according to any one of claims 1-5, wherein the hyaluronic acid substance comprises unmodified hyaluronic acid or a salt thereof and / or modified hyaluronic acid or a salt thereof.

[0014] 7. The composition according to claim 6, wherein the modified hyaluronic acid comprises acetylated hyaluronic acid, cross-linked hyaluronic acid or a salt thereof, alkylated hyaluronic acid or a salt thereof.

[0015] 8. The composition according to any one of claims 1-7, wherein the composition further comprises cosmetically acceptable excipients.

[0016] 9. A method for stabilizing blue copper peptide, the method comprising using the composition according to any one of claims 1-8.

[0017] 10. A cosmetic method for non-therapeutic purposes, the method comprising using the composition of any one of claims 1-8 on the skin.

[0018] Beneficial effects

[0019] The cosmetic composition provided in this application can overcome the influence of hyaluronic acid on the stability of copper peptide, thereby obtaining a stable cosmetic composition containing hyaluronic acid and copper peptide. Attached Figure Description

[0020] Figure 1 This is the absorption spectrum of the blue copper peptide solution in the 200-800 nm range.

[0021] Figure 2 This is the absorption spectrum of sodium hyaluronate solution in the 200-800 nm range. Detailed Implementation

[0022] The present application is further illustrated below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present application and are not intended to limit the present application.

[0023] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The present application is further described below with reference to specific embodiments, but is not intended to limit the scope of the application.

[0024] This application provides a cosmetic composition comprising copper peptide and hyaluronic acid-like substances.

[0025] The hyaluronic acid-like substances have a molecular weight greater than 5 kDa and less than or equal to 900 kDa, for example, 10 kDa, 20 kDa, 30 kDa, 40 kDa, 100 kDa, 200 kDa, 300 kDa, 400 kDa, 500 kDa, 600 kDa, 700 kDa, 800 kDa, and 900 kDa.

[0026] In one or more embodiments, the molecular weight of the hyaluronic acid-like substance is 30kDa-900kDa.

[0027] In the composition of this application, hyaluronic acid-like substances can balance the density of hyaluronic acid complexation sites and the steric hindrance of molecules, thereby ensuring that when mixed with copper peptides, they do not affect the stability of blue copper peptides, and may even improve the stability of blue copper peptides themselves, so that the composition has good stability.

[0028] The hyaluronic acid-based substances include unmodified hyaluronic acid or its salts and / or modified hyaluronic acid or its salts.

[0029] The unmodified hyaluronic acid or its salt can be sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, calcium hyaluronate, etc. The modified hyaluronic acid or its salt can be acetylated hyaluronic acid or its salt, cross-linked hyaluronic acid or its salt, alkylated hyaluronic acid or its salt, and preferably unmodified hyaluronic acid or its salt.

[0030] The copper peptide in this application can be any commercially available copper peptide, or it can be a copper peptide prepared by methods known in the art.

[0031] In the composition of this application, the hyaluronic acid and copper peptides may be present in amounts as needed.

[0032] In one or more embodiments, the content of the hyaluronic acid substance in the composition is greater than 0 wt% and less than or equal to 10 wt%, and may be further greater than 0 wt% and less than or equal to 5 wt%, and may be further 0.3 wt% to 3 wt%.

[0033] In one or more embodiments, the mass ratio of the copper peptide to the hyaluronic acid in the composition is 1:1 to 1:9, for example, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, or 1:9.

[0034] In one or more embodiments, the composition includes a copper peptide and sodium hyaluronate, wherein the sodium hyaluronate has a molecular weight greater than 5 kDa and less than or equal to 900 kDa, and the mass ratio of the copper peptide to the hyaluronic acid is 1:1 to 1:9.

[0035] In one or more embodiments, the composition comprises a copper peptide and sodium hyaluronate, wherein the sodium hyaluronate has a molecular weight of 40kDa-900kDa and the mass ratio of the copper peptide to the sodium hyaluronate is 1:1-1:9.

[0036] In one or more embodiments, the composition comprises a copper peptide and acetylated sodium hyaluronate, wherein the acetylated sodium hyaluronate has a molecular weight greater than 5 kDa and less than or equal to 40 kDa, and the mass ratio of the copper peptide to the hyaluronic acid is 1:1 to 1:9.

[0037] In one or more embodiments, the composition comprises a copper peptide and acetylated sodium hyaluronate, wherein the acetylated sodium hyaluronate has a molecular weight of 40kDa-900kDa, and the mass ratio of the copper peptide to the hyaluronic acid is 1:1-1:9.

[0038] The composition also includes cosmetically acceptable excipients. These excipients include, but are not limited to, thickeners, colorants, preservatives, cleansing and skincare aids, oils, emulsifiers, moisturizers, skincare functional ingredients, fragrances, etc. The thickeners may exclude carbomer and sodium polyacrylate. The cleansing and skincare aids include, but are not limited to, solvents and antioxidants.

[0039] The compositions described in this application can be formulated into commonly used cosmetic formulations such as aqueous solutions, oils, emulsions, creams, gels, films, powders, and solids.

[0040] This application also provides a method for stabilizing blue copper peptide, the method comprising using the above-described composition.

[0041] This application also provides a cosmetic method for non-therapeutic purposes, the method comprising applying the above-described composition to the skin.

[0042] Example

[0043] Information on the raw materials and equipment used in the embodiments of this application is shown in the table.

[0044] Table 1

[0045]

[0046]

[0047] Example 1

[0048] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.003g of sodium hyaluronate (HA-TLM). 3-5 Add deionized water to 1g to obtain the composition of this application.

[0049] Example 2

[0050] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.003g of sodium hyaluronate (HA-TLM). 20-40 Add deionized water to 1g to obtain the composition of this application.

[0051] Example 3

[0052] According to the amounts of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh 0.003 g of blue copper peptide and 0.003 g of sodium hyaluronate (HA-TP), add deionized water to 1 g, and obtain the composition of this application.

[0053] Example 4

[0054] According to the amounts of blue copper peptide, acetylated sodium hyaluronate, and molecular weight shown in Table 2, weigh 0.003g of blue copper peptide and 0.003g of acetylated sodium hyaluronate (AcHA), add deionized water to 1g, and obtain the composition of this application.

[0055] Example 5

[0056] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.027g of sodium hyaluronate (HA-TLM). 20-40 Add deionized water to 1g to obtain the composition of this application.

[0057] Example 6

[0058] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.027g of sodium hyaluronate (HA-TLM). 3-5 Add deionized water to 1g to obtain the composition of this application.

[0059] Example 7

[0060] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.03g of sodium hyaluronate (HA-TLM). 20-40 Add deionized water to 1g to obtain the composition of this application.

[0061] Example 8

[0062] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.03g of sodium hyaluronate (HA-TLM). 3-5 Add deionized water to 1g to obtain the composition of this application.

[0063] Example 9

[0064] According to the dosage of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh out 0.003g of blue copper peptide and 0.003g of sodium hyaluronate (HA-TLM). 20-40 Weigh 0.003g butanediol, 0.003g pentanediol and 0.003g panthenol, add deionized water to 1g to obtain the composition of this application.

[0065] Comparative Example 1

[0066] According to the amounts of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh 0.003g of blue copper peptide and 0.003g of sodium hyaluronate (mini HA), add deionized water to 1g, and obtain the composition of this application.

[0067] Comparative Example 2

[0068] According to the amounts of blue copper peptide, sodium hyaluronate, and molecular weight shown in Table 2, weigh 0.003g of blue copper peptide and 0.003g of sodium hyaluronate (HA-THM), add deionized water to 1g, and obtain the composition of this application.

[0069] The specific conditions of the above embodiments are shown in Table 2.

[0070] Table 2

[0071] serial number Amount of blue copper peptide added (g) Molecular weight of hyaluronic acid (kDa) Amount of hyaluronic acid salt added (g) Example 1 0.003 40 0.003 Example 2 0.003 300 0.003 Example 3 0.003 900 0.003 Example 4 0.003 40(AcHA) 0.003 Example 5 0.003 300 0.027 Example 6 0.003 40 0.027 Example 7 0.003 300 0.03 Example 8 0.003 40 0.03 Example 9 0.003 300 0.003 Comparative Example 1 0.003 5 0.003 Comparative Example 2 0.003 1500 0.003

[0072] Test case

[0073] The stability of a composition can be determined by observing changes in its UV-Vis absorbance. According to Beer-Lambert's law, the absorbance of water-soluble components is directly proportional to their concentration. Therefore, by recording the absorbance changes of the aged sample at the characteristic absorption peak of the copper peptide, the degree of degradation can be determined.

[0074] Because copper peptide itself also degrades, when judging the stability of the combination of HA and copper peptide, if the change in absorbance of the combination is less than the change in absorbance of copper peptide itself, the combination is considered stable.

[0075] Using a Mettler Toledo UV5NANO ultra-micro UV-Vis spectrophotometer, the copper peptide solution dilution 1 was first scanned, and the absorption spectrum of the blue copper peptide solution in the 200-800 nm range was obtained as follows. Figure 1 As shown. Diluent 1 consists of 0.3% (w / w) blue copper peptide and water, while diluent 2 consists of 0.3% (w / w) sodium hyaluronate (HA-TLM). 20-40 It is composed of ) and water.

[0076] Therefore, the characteristic absorption peaks of the copper peptide were selected at 242.8 nm, 247.6 nm, 260.4 nm, and 285.4 nm in the ultraviolet region, and 605.0 nm in the visible region. This is because Cu... 2+ After forming a complex with the peptide, Cu causes a red shift in the peptide's absorption in the ultraviolet region relative to the peptide monomer, which is a characteristic absorption peak of this coordination compound. In the visible light region, Cu... 2+ Ions have characteristic absorption peaks, which are related to their oxidation state.

[0077] The compositions prepared in the above examples were subjected to stability testing. Specifically, after accelerated aging at 45 degrees Celsius for 2 weeks, the absorbance values ​​of the characteristic absorption peaks at wavelengths of λ = 242.8 nm, λ = 247.6 nm, λ = 260.4 nm, λ = 285.4 nm, and λ = 605.0 nm were read. The absorbance change ratios of each composition were calculated, and the results are shown in Table 3.

[0078] Table 3

[0079] Sample number Delta-Ua Delta-Ub Delta-Uc Delta-Ud Delta-VS Diluent 1 8.78％ 7.73％ 6.63％ 6.88％ 24.88％ Diluent 2 —— —— —— —— —— Example 1 4.83％ 5.09％ 3.78％ 3.45％ 6.08％ Example 2 5.02％ 3.19％ 2.65％ 5.40％ 2.65％ Example 3 6.01％ 6.61％ 4.78％ 4.12％ 3.52％ Example 4 6.83％ 7.92％ 3.03％ 9.57％ 5.11％ Example 5 8.14％ 7.66％ 6.51％ 6.78％ 6.95％ Example 6 6.22％ 6.70％ 5.11％ 4.34％ 22.19％ Example 7 10.20％ 7.95％ 8.20％ 7.45％ 7.38％ Example 8 9.43％ 9.31％ 7.46％ 6.03％ 33.36％ Example 9 5.33％ 3.32％ 2.69％ 5.51％ 2.72％ Comparative Example 1 11.72％ 7.89％ 6.85％ 10.48％ 30.34％ Comparative Example 2 6.42％ 11.90％ 9.43％ 12.31％ 48.22％

[0080] Where Delta_Ua represents the ratio of absorbance change of each composition at λ = 242.8 nm, and the specific calculation method is as follows:

[0081] |Absorbance value after aging – Absorbance value when the composition is fully prepared| / Absorbance value when the composition is fully prepared

[0082] In this context, "||" represents taking the absolute value.

[0083] Delta_Ub represents the ratio of absorbance changes for each composition at λ = 247.6 nm, and the specific calculation method is as follows:

[0084] |Absorbance value after aging – Absorbance value when the composition is fully prepared| / Absorbance value when the composition is fully prepared

[0085] In this context, "||" represents taking the absolute value.

[0086] Delta_Uc represents the ratio of absorbance changes of each composition at λ = 260.4 nm, and the specific calculation method is as follows:

[0087] |Absorbance value after aging – Absorbance value when the composition is fully prepared| / Absorbance value when the composition is fully prepared

[0088] In this context, "||" represents taking the absolute value.

[0089] Delta_Ud represents the ratio of absorbance changes of each composition at λ = 285.4 nm, and the specific calculation method is as follows:

[0090] |Absorbance value after aging – Absorbance value when the composition is fully prepared| / Absorbance value when the composition is fully prepared

[0091] In this context, "||" represents taking the absolute value.

[0092] Delta_VS represents the ratio of absorbance changes for each composition at λ = 605.0 nm, and the specific calculation method is as follows:

[0093] |Absorbance value after aging – Absorbance value when the composition is fully prepared| / Absorbance value when the composition is fully prepared

[0094] In this context, "||" represents taking the absolute value.

[0095] The smaller the absorbance change value, the better the stability of the composition.

[0096] In dilution 2, hyaluronic acid or its salts showed no change in absorbance at the aforementioned wavelengths. Figure 2 Sodium hyaluronate (HA-TLM) 20-40 The UV-Vis absorption curve of the solution.

[0097] Table 3 shows that, compared with diluent 1, comparative examples 1-3 reduce the stability of the composition. However, when using the composition described in the embodiments of this application, not only can the stability of the composition be improved, but the stability of the blue copper peptide itself can also be improved.

Claims

1. A cosmetic composition containing a stable copper peptide, characterized in that, The composition comprises copper peptide and hyaluronic acid, wherein the mass ratio of copper peptide to hyaluronic acid is 1:1 to 1:

9. The content of hyaluronic acid in the composition is 0.3wt% to 3wt%, and the hyaluronic acid is unmodified hyaluronic acid or its salt and / or acetylated hyaluronic acid or its salt, wherein the molecular weight of the hyaluronic acid is 30 kDa to 900 kDa.

2. The composition according to claim 1, characterized in that, The composition also includes cosmetically acceptable excipients.

3. A method for stabilizing blue copper peptides, the method comprising using the composition of claim 1 or 2.

4. A cosmetic method for non-therapeutic purposes, said method comprising using the composition of claim 1 or 2 on the skin.

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