A light red soothing repair lotion and preparation method thereof

Abstract

The present invention discloses a light red soothing and repairing lotion and a preparation method thereof, and belongs to the field of dressing and makeup products. The extraction raw materials include sodium dextran sulfate, ectoine, and plant extract fermentation products; the plant extract fermentation products use Centella asiatica extract, sea buckthorn extract, lily extract, and rosemary extract as substrates, and use Trichoderma dinghuensis and Rhizoma Dictyophora to mix and ferment. The light red soothing and repairing lotion provided by the present invention has significant light red, soothing, and repairing effects.

Description

Technical Field

[0001] The invention belongs to the field of dressing and makeup products, and in particular relates to a light red soothing and repairing lotion and a preparation method thereof. Background Art

[0002] In today's skin care market, with the influence of environmental pollution, life pressure and improper skin care habits, skin sensitivity, redness, damage and other problems are becoming more and more common, and consumers are in urgent need of skin care products that can effectively alleviate and repair these problems. However, traditional skin care products often focus on a single moisturizing or whitening effect, which is difficult to meet consumers' expectations for comprehensive soothing and repairing.

[0003] Plant extract fermentation refers to extracting active ingredients from plants through traditional methods (such as solvent extraction, steam distillation, etc.), and then using microorganisms for fermentation. Compared with the fermentation method using plants as raw materials, it has more significant advantages. Specifically, through the preliminary extraction step, plant extract fermentation can accurately focus on the active ingredients in the plant, so that these ingredients can be more effectively converted and enriched in the subsequent fermentation process. This highly targeted treatment method not only improves the fermentation efficiency, but also ensures the stability and consistency of product quality. In addition, plant extract fermentation can remove impurities and harmful substances that may exist in the raw materials, further reducing the toxicity and irritation of the product, and improving the safety and applicability of the product.

[0004] Therefore, there is an urgent need to provide a fermentation product with plant extracts as substrates for use in cosmetics to fill the market gap. Summary of the invention

[0005] The invention provides a light red soothing and repairing lotion and a preparation method thereof, which can achieve the effects of light redness, soothing and repairing through natural ingredients extracted from plants.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a method for preparing a plant extract fermentation product, the specific steps of which are as follows:

[0008] S1, taking 1-2 parts by weight of Centella asiatica extract, 2-5 parts by weight of Hippophae rhamnoides fruit extract, 0.2-0.7 parts by weight of Rosemary extract, and 0.1-0.5 parts by weight of Lilium sibiricum extract, and dissolving them in 2000 parts by weight of water to obtain a mixed liquid A;

[0009] S2, adding peanut shell powder, fish maw powder, and corn pollen in a mass ratio of 1:0.1-0.4:3-5 to the mixed solution A, stirring evenly to obtain a mixed solution B, wherein the total amount of the peanut shell powder, fish maw powder, and corn pollen added accounts for 15% of the mixed solution A;

[0010] S3, stirring the mixed solution B evenly, and then sterilizing the mixed solution B to obtain a mixed solution C;

[0011] S4, adding red bamboo fungus and Dinghu scale mushroom to the mixed solution C to obtain a mixed solution D, wherein the amount of the red bamboo fungus and Dinghu scale mushroom added is 8-10% of the mass percentage of the mixed solution B, wherein the mass ratio of the red bamboo fungus to the Dinghu scale mushroom is 1:0.2-0.4;

[0012] S5. Ferment the mixed solution D and filter it; filter out the mycelium by the first filtration, and then filter the residue for the second time to obtain the filtrate; then sterilize the mycelium and the filtrate and freeze-dry them to obtain the mycelium fermentation product E and the fermentation liquid product H; the fermentation temperature is 20-30°C, the fermentation starting pH is 5-6, the fermentation ventilation volume is 5-7L / min, the fermentation time is 18h, and the fermentation stirring speed is 210r / min for the first 4h and 70r / min for the last 14h.

[0013] The plant extract fermentation product is obtained by mixing the mycelium fermentation product E and the fermentation liquid fermentation product H in a weight ratio of 0.5:4-6.

[0014] In a second aspect, the present invention provides a light red soothing repairing composition, the specific mass fraction ratio of which is as follows:

[0015] 1-5 parts of sodium dextran sulfate;

[0016] Ecdoin 0.01-0.1 part;

[0017] 1-3 servings of fermented plant extracts

[0018] In a third aspect, the present invention provides a light red soothing repairing lotion, and the specific preparation method thereof is as follows:

[0019] Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 0.5-5% light red soothing repair composition, homogenize and sterilize.

[0020] In the present invention, the fungi Rhododendron erythrorhizome and Trichoderma lucidum are used to carry out mixed fermentation of plant extracts, and the natural active substances obtained by the mixed fermentation are supplemented with ectoine and sodium dextran sulfate to provide better reddening, soothing and repairing effects for the skin. In addition, due to the additional amino acids and terpenoid compounds provided by the fungal fermentation, the overall composition is more gentle and non-irritating.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention innovatively uses plant extracts as fermentation substrates instead of directly using the plants themselves as fermentation substrates. Such operation can make the fermentation products more consistent and more conducive to the utilization and transformation of microorganisms. In addition, the ingredients in plant raw materials are more complex and are very likely to contain pesticide residues, heavy metals and other harmful substances. Such substances may not only affect the fermentation effect, but may also have an adverse effect on the quality and safety of the final product.

[0022] In addition, the present invention pioneered the use of dual fungi for fermentation. Different microorganisms have different metabolic pathways and production capabilities during the fermentation process. During the dual fungi mixed fermentation process, the two may cooperate with each other to jointly complete a metabolic pathway that is difficult to complete by a single strain of bacteria, thereby improving the fermentation efficiency and bringing unexpected results. Secondly, during the dual fungi mixed fermentation process, the synergistic effect of the two will produce more complex metabolites, improve the quality and diversity of the products, and also reduce by-products and improve product purity. DETAILED DESCRIPTION

[0023] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0024] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0025] Some raw materials and their sources are as follows:

[0026] Dextran sulfate sodium: purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

[0027] Ecdoin: purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

[0028] Centella asiatica extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

[0029] Seabuckthorn fruit extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

[0030] Rosemary extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

[0031] Lily extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

[0032] Dictyophora rubrovolvata M. Zang, DG Ji & X.X. Liu: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., BMZ098545.

[0033] Dinghulin Agaricus (Pholiota dinghuensis ZSBi): purchased from Ningbo Mingzhou Biotechnology Co., Ltd., catalog number BMZ092462.

[0034] Dictyophora indusiata: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number BMZ079473.

[0035] Pholiota nameko (T.Ito) S.Ito & S.Imai: purchased from Ningbo Mingzhou Biotechnology Co., Ltd., catalog number BMZ092455.

[0036] Preparation, comprising the following steps:

[0037] The method for preparing plant extract fermentation product comprises the following specific steps:

[0038] S1, taking 1-2 parts by weight of Centella asiatica extract, 2-5 parts by weight of Hippophae rhamnoides fruit extract, 0.2-0.7 parts by weight of Rosemary extract, and 0.1-0.5 parts by weight of Lilium sibiricum extract, and dissolving them in 2000 parts by weight of water to obtain a mixed liquid A;

[0039] S2, adding peanut shell powder, fish maw powder, and corn pollen in a mass ratio of 1:0.1-0.4:3-5 to the mixed solution A, stirring evenly to obtain a mixed solution B, wherein the total amount of the peanut shell powder, fish maw powder, and corn pollen added accounts for 15% of the mixed solution A;

[0040] S3, stirring the mixed solution B evenly, and then sterilizing the mixed solution B to obtain a mixed solution C;

[0041] S4, adding red bamboo fungus and Dinghu scale mushroom to the mixed solution C to obtain a mixed solution D, wherein the amount of the red bamboo fungus and Dinghu scale mushroom added is 8-10% of the mass percentage of the mixed solution B, wherein the mass ratio of the red bamboo fungus to the Dinghu scale mushroom is 1:0.2-0.4;

[0042] S5. Ferment the mixed solution D and filter it; filter out the mycelium by the first filtration, and then filter the residue for the second time to obtain the filtrate; then sterilize the mycelium and the filtrate and freeze-dry them to obtain the mycelium fermentation product E and the fermentation liquid product H; the fermentation temperature is 20-30°C, the fermentation starting pH is 5-6, the fermentation ventilation volume is 5-7L / min, the fermentation time is 18h, and the fermentation stirring speed is 210r / min for the first 4h and 70r / min for the last 14h.

[0043] The plant extract fermentation product is obtained by mixing the mycelium fermentation product E and the fermentation liquid fermentation product H in a weight ratio of 0.5:4-6.

[0044] Example 1: Follow steps S1-S5, wherein in step S1: take 1 part by mass of Centella asiatica extract, 2 parts by mass of sea buckthorn fruit extract, 0.2 parts by mass of rosemary extract, and 0.1 parts by mass of lily extract; in step S2: the mass fraction ratio of peanut shell powder, fish maw powder, and corn pollen is 1:0.4:5; in step S4: the amount of red-sprung bamboo fungus and Dinghu agaricus added is 8% of the mass percentage of mixed solution B; the mass ratio of red-sprung bamboo fungus to Dinghu agaricus is 1:0.2.

[0045] Example 2: Follow steps S1-S5, wherein, in step S1: take 2 parts by mass of Centella asiatica extract, 5 parts by mass of sea buckthorn fruit extract, 0.7 parts by mass of rosemary extract, and 0.5 parts by mass of lily extract; in step S2: the mass fraction ratio of peanut shell powder, fish maw powder, and corn pollen is 1:0.1:3; in step S4: the amount of red-sprung bamboo fungus and Dinghu agaricus added is 10% of the mass percentage of mixed solution B; the mass ratio of red-sprung bamboo fungus to Dinghu agaricus is 1:0.4.

[0046] Example 3: Follow steps S1-S5, wherein in step S1: take 1.5 parts by mass of Centella asiatica extract, 3.5 parts by mass of sea buckthorn fruit extract, 0.5 parts by mass of rosemary extract, and 0.3 parts by mass of lily extract; in step S2: the mass fraction ratio of peanut shell powder, fish maw powder, and corn pollen is 1:0.3:4; in step S4: the amount of red-suspension bamboo fungus and Dinghu agaricus added is 9% of the mass percentage of mixed solution B; the mass ratio of red-suspension bamboo fungus and Dinghu agaricus is 1:0.3.

[0047] Comparative Example 1: The difference from Example 3 is that step S2 does not contain peanut shell powder, fish maw powder, and corn pollen, and the remaining steps are the same as Example 3.

[0048] Comparative Example 2: The difference from Example 3 is that step S1 does not contain Centella asiatica extract, and the remaining steps are the same as Example 3.

[0049] Comparative Example 3: The difference from Example 3 is that step S1 does not contain seabuckthorn fruit extract, and the remaining steps are the same as Example 3.

[0050] Comparative Example 4: The difference from Example 3 is that step S1 does not contain rosemary extract, and the remaining steps are the same as Example 3.

[0051] Comparative Example 5: The difference from Example 3 is that step S1 does not contain lily extract, and the remaining steps are the same as Example 3.

[0052] Comparative Example 6: The difference from Example 3 is that step S1 does not contain Centella asiatica extract and rosemary extract, and the remaining steps are the same as Example 3.

[0053] Comparative Example 7: The difference from Example 3 is that step S1 does not contain seabuckthorn fruit extract and lily extract, and the remaining steps are the same as Example 3.

[0054] Comparative Example 8: The difference from Example 3 is that step S2 does not contain peanut shell powder, and the remaining steps are the same as Example 3.

[0055] Comparative Example 9: The difference from Example 3 is that step S2 does not contain corn pollen, and the remaining steps are the same as Example 3.

[0056] Comparative Example 10: The difference from Example 3 is that step S2 does not contain fish maw powder, and the remaining steps are the same as Example 3.

[0057] Comparative Example 11: The difference from Example 3 is that step S4 does not contain red-rooted bamboo fungus, and the remaining steps are the same as Example 3.

[0058] Comparative Example 12: The difference from Example 3 is that step S4 does not contain Trichoderma, and the remaining steps are the same as Example 3.

[0059] Comparative Example 13: The difference from Example 3 is that in step S4, the red-sprung bamboo fungus and the Dinghu Trichoderma are replaced with long-skirted bamboo fungus and light-capped Trichoderma, and the remaining steps are the same as Example 3.

[0060] Comparative Example 14: The difference from Example 3 is that the final product is product E, and the remaining steps are the same as Example 3.

[0061] Comparative Example 15: The difference from Example 3 is that the final product is product H, and the remaining steps are the same as Example 3. A light red soothing repairing composition, the specific mass fraction ratio of which is shown in Table 1.

[0062] Table 1

[0063]

[0064]

[0065] A light red soothing repairing lotion and a preparation method thereof are prepared as follows:

[0066] Example 9: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 0.5% of the light red soothing repairing composition of Example 5, homogenize and sterilize.

[0067] Example 10: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 3% of the light red soothing repairing composition of Example 5, homogenize and sterilize.

[0068] Example 11: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 5% of the light red soothing repairing composition of Example 5, homogenize and sterilize.

[0069] Example 12: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 3% of the light red soothing repairing composition of Example 4, homogenize and sterilize.

[0070] Example 13: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 3% of the light pink soothing repairing composition of Example 6, homogenize and sterilize.

[0071] Example 14: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 3% of the light red soothing repairing composition of Example 7, homogenize and sterilize.

[0072] Example 15: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 3% of the light red soothing repairing composition of Example 8, homogenize and sterilize.

[0073] Comparative Example 33: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, and sterilize.

[0074] Comparative Example 34: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract in a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 10% light red soothing repair composition, homogenize and sterilize.

[0075] Safety Test

[0076] With reference to the 2015 Technical Specifications for Safety of Cosmetics, the skin care products of Examples 9 to 15 were evaluated for cosmetic irritation. The test method was a skin patch test, and 70 randomly distributed people aged 16 to 65 were tested.

[0077] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0078] Evaluation criteria:

[0079] Grade 0: negative reaction;

[0080] Grade 1: Suspicious reaction, only slight erythema;

[0081] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0082] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0083] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0084] Test results: Skin reactions were negative in all subjects.

[0085] The above test results show that the light red soothing repair lotion provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0086] Cytotoxicity assay

[0087] Take the human immortalized epidermal cell line (HaCaT) with stable growth state and adjust the cell concentration to 1×10 5 / mL, 100μL per well. Inoculate into 96-well cell culture plates (except the outermost wells and blank control wells). Add 200μL of PBS to the outermost wells of the cell culture plate to reduce the evaporation of the culture medium. Place in a carbon dioxide incubator and culture at 37℃±1℃, CO2 concentration 5.0%±1% until the cell fusion reaches more than 90%. Add samples, the sample concentration is 10mg / L, set up blank control and sample groups, 100μL per well. Start adding samples (sample group) and PBS (blank control) at the same time interval between each two columns to ensure that the intervention time of each test substance on the cells is 5min. After 5min, remove the test substances in turn, add PBS to each well to wash the test substances, and wash twice. When using a vacuum suction pump or a discharge gun to remove the liquid in the well, be careful to avoid sucking away the surviving cells and affecting the experimental results. Add 100μL of complete culture medium and 10μL of CCK-8 solution to each well, and place in an incubator to culture for about 2h. At a wavelength of 450 nm, the absorbance (OD) value was measured and the cell viability was calculated; the results are shown in Table 2.

[0088] Formula (1)

[0089] Table 2 Cytotoxicity test

[0090] Experimental Group HaCaT survival rate Example 9 97 Example 10 96 Embodiment 11 95 Example 12 95 Example 13 98 Embodiment 14 96 Embodiment 15 95 blank (100)％

[0091] According to the experimental results, the cell survival rate is maintained above 90%. The combination of the present experiment has little effect on cell viability.

[0092] Determination of Antioxidant Activity (AOA) in PUFA Peroxidation System

[0093] Fe 2+ The peroxidation model of very low density lipoprotein (VLDL) and low density lipoprotein (DL) superunsaturated fatty acids (PUFA) at the C2 position of egg yolk phospholipids was induced, and the anti-lipid peroxidation effect of the samples was determined by the thiobarbituric acid method.

[0094] Yolk suspension: remove the white of fresh eggs, and prepare the egg yolk into a 1:1 suspension with an equal volume of pH 7.45, 0.1 mol / L sodium phosphate buffer (PBS). Stir magnetically for 10 min, and then dilute with PBS buffer to a suspension with a volume ratio of 1:25 (refrigerate for later use).

[0095] Determination method: Add 0.4mL of egg yolk suspension, 0.1mL of 10g / L sample solution, 0.4mL of FeSO4 (25mmol / L), and 3.1mL of PBS (0.1mo / L; pH 7.45) to a stoppered test tube, mix well and oscillate at 37℃ for 15min. After taking out, add 1mL of trichloroacetic acid (mass fraction 20%), centrifuge at 3500r / min for 10min, aspirate 2mL of supernatant, add 1mL of TBA (mass fraction 0.8%), stopper and place in a boiling water bath for 15min. After cooling, adjust to zero with a blank tube (replaced with 3mL of distilled water), and determine the absorbance colorimetrically at a wavelength of 532nm. The absorbance of the tube without sample (replaced with 0.1mL of 1mg / mLVc) is A0, and the absorbance of the sample tube is A.

[0096]

[0097] Note: AOA is the peroxidation inhibition rate of the sample solution in the yolk lipoprotein lipid peroxidation system; A is the average absorbance after adding the example or comparative example; A0 is the average absorbance of the blank group.

[0098] Table 3

[0099]

[0100] According to the results of Examples 4-8 in Table 3, the compositions provided by the embodiments of the present invention have good antioxidant properties, especially Example 4 has a better effect.

[0101] By comparing the results of Example 4 with Comparative Examples 3 and 32, it can be seen that the combination of different components can produce a synergistic effect on the antioxidant effect, and the antioxidant effect achieved by the present invention cannot be achieved if any one of the components is omitted.

[0102] By comparing the results of Example 4 with Comparative Examples 16-30, it can be seen that the plant extract fermentation products with different extraction processes and other compositions have a synergistic effect on the antioxidant efficacy, and changing the extraction process cannot achieve the antioxidant efficacy achieved by the present invention.

[0103] Inflammatory factor inhibition test

[0104] TNF-α is the most common inflammatory factor in inflammatory reactions. It can induce the phenotype of vascular endothelial cells to change to an inflammatory type, leading to changes in the body's metabolism and hemodynamics, and promoting the production of inflammatory mediators. Inflammatory mediators can increase vascular permeability, causing local congestion and edema, and may stimulate nerve endings to cause itching and pain. Therefore, the inhibition of TNF-α helps soothe and repair the skin.

[0105] RAW264.7 cells grown to the logarithmic phase were cultured at 1.5×10 5Cells were inoculated at a density of 100 μg / mL in a 24-well plate, with 800 μL of cell suspension per well, and cultured in a 37°C, 5% CO2 incubator for 24 hours. When the cells grew to near fusion, 50 μL of the sample concentration of 10 mg / L was administered, with 3 replicates per group. The positive control was 50 μL of 10 μg / mL dexamethasone, and 1 μg / mL lipopolysaccharide (LPS) was used for stimulation 1 hour later. After incubation in a cell culture incubator for 24 hours, the cell culture fluid was transferred to a centrifuge tube, centrifuged at 5000 r / min for 10 minutes, and the cell supernatant was collected and tested according to the instructions of the mouse TNF-α enzyme-linked immunosorbent assay kit.

[0106] Formula (3)

[0107] Table 4 Results of RAW secretion of TNF-α

[0108]

[0109]

[0110] From the results analysis of Table 4: From the results of Examples 4-8, it can be seen that the composition provided by the embodiments of the present invention has good TNF-α inhibitory effect, especially the effect of Example 4 is better, which means that using Example 3 as the fermentation process parameter can effectively improve the inhibitory effect of the composition on TNF-α.

[0111] From the results of Example 4 and Comparative Examples 31-32, it can be seen that there is a certain synergistic effect when using the components defined in the present invention, and the effect achieved by the present invention cannot be achieved without omitting any one of them.

[0112] By comparing the results of Example 4 with Comparative Examples 16-30, it can be seen that the use of the process defined by the present invention can achieve the maximum efficacy of the present invention in inhibiting TNF-α, and any process not defined by the present invention cannot achieve the effect achieved by the present invention.

[0113] Effects of the composition on the migration of Hacat cells through scratch wound

[0114] The degree of cell migration has an important impact on wound healing and skin repair. In the process of wound healing, cell migration is an important step for epidermal cells to cover the wound, so the higher the cell migration rate, the faster the wound heals. At the same time, cell migration is also involved in the repair process after skin damage. If cell migration is impaired, it will lead to skin repair disorders, which in turn cause various skin diseases.

[0115] Inoculate cells into 12-well plates at the corresponding inoculation density and incubate overnight at 37°C in a CO2 incubator. When the cell plating rate in the 12-well plate reaches about 80%, scratch the sample group and the solvent control group. After the scratching, wash the cells with PBS to remove dead cells, add fresh EGF-free keratinocyte culture solution, add 50μL of 10mg / L according to Example 4-8 and Comparative Example 16-32, and take photos and record using an inverted microscope. Incubate in a 37°C, 5% CO2 incubator for 24h, take photos and record again, and use ImageJ software for statistical analysis.

[0116] Table 5 Hacat cell migration rate

[0117]

[0118]

[0119] From the results analysis of Table 5: From the results of Examples 4-8, it can be seen that the composition provided by the embodiments of the present invention has the effect of significantly improving the cell migration rate, especially the effect of Example 5 is better, which means that using Example 3 as the process parameter can effectively improve the cell migration rate.

[0120] Comparing Example 5 with Comparative Examples 31-32, Example 5 has the best effect. There is a certain synergistic effect when using the components defined in the present invention. The effect achieved by the present invention cannot be achieved if any one of them is omitted.

[0121] By comparing the results of Example 5 with Comparative Examples 16-30, it can be seen that the use of the fermentation process defined in the present invention can enable the present invention to achieve the maximum effect in improving cell migration rate, and changing any plant extract fermentation process defined in the present invention cannot achieve the effect achieved by the present invention.

[0122] 1. Human body 30s instant soothing effect test

[0123] Volunteer selection criteria: a) healthy volunteers aged 18 to 60 years old; b) subjects with no skin lesions / dermatitis or other factors that affect the measurement at the test site; c) those with sensitive and dry faces; d) those with positive lactic acid stinging test.

[0124] The number of volunteers is guaranteed to be more than 30, and the subjects should mainly engage in indoor activities to avoid long-term exposure to light and high temperature or cold environments.

[0125] 100 volunteers aged 22-55 with sensitive facial skin (lactic acid stinging test score ≥ 3 points on both sides of the nose) and dry East Asian skin were selected. A sample group and a blank group (i.e., a model control group: no sample was applied) were set up, and the test areas were randomly distributed on the left and right faces of the volunteers. After using the essence of the present invention for 30 seconds, the red image was collected by the skin facial image analyzer VISIA 7, and the skin redness a* value was analyzed by Image-Pro Plus (IPP) analysis software to evaluate its moisturizing and soothing effects. The laboratory temperature was 21±1°C; the relative humidity was 50%±10%.

[0126] The experiment adopted the self-before and after control method and the blank group control method. The test cycle was 30s, and the skin image collection times were 2 times, 0s and 30s respectively. Set up a sample group and a blank group (i.e., model control group: no sample was applied), and the test areas were randomly distributed on the left and right faces of the volunteers. Apply the sample at a dosage of 0.25g / half face. Select qualified subjects according to the requirements, and when the volunteers visit, sign the volunteer informed consent form with them, collect skin images and questionnaire data after using the product for 30s. When visiting, after washing the face with the designated cleanser, sit quietly in the test environment for about 20-30 minutes. After the sitting is over, start the test.

[0127] With the help of facial image analyzer VISIA7( Company, USA), the instrument uses 3 different light sources to capture facial images and can intuitively observe changes in facial skin conditions.

[0128] The red image a* value was analyzed using Image-Pro Plus software.

[0129] Skin redness a* value: The a* value represents the redness of the skin. An increase in the a* value indicates an increase in the vascular reactivity of the skin.

[0130] The data were analyzed using SPSS19.0 data analysis software.

[0131] Table 6 Skin redness a* experimental results

[0132]

[0133]

[0134] From the analysis of the results in Table 6: Comparing Examples 9-15 and Comparative Examples 33-34, the results show that the skin redness a* of Examples 9-15 is reduced, and the reduction degree is the highest in Example 10, indicating that the skin soothing effect of the emulsion prepared using the composition of the present invention does not show a linear trend with the addition amount.

[0135] Comparative Example 10 and Comparative Example 34 show that too high an addition amount of the composition cannot significantly reduce the average value of skin redness, and the addition amount defined in the present invention can maximize the reduction of the skin redness a* value by the emulsion.

[0136] Comparative Example 10, Comparative Example 33 and Blank indicate that the use of the composition prepared by the present invention can achieve maximum efficacy in reducing the a* value of skin redness of the lotion.

[0137] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A light red soothing repair composition, characterized in that: The composition comprises the following compositions in parts by weight: 1-5 parts of sodium dextran sulfate; Ecdoin 0.01-0.1 part; 1-3 parts of plant extract fermentation product; The steps for preparing the plant extract fermentation product are as follows: S1, taking Centella asiatica extract, seabuckthorn fruit extract, rosemary extract, and lily extract and dissolving them in water to obtain a mixed liquid A; S2, adding peanut shell powder, fish maw powder and corn pollen to the mixed solution A, stirring evenly to obtain a mixed solution B; S3, sterilizing the mixed solution B to obtain a mixed solution C; S4, adding the bacterial strain to the mixed solution C to obtain a mixed solution D; S5, fermenting the mixed solution D, filtering, sterilizing, and freeze-drying to obtain mycelium E and fermentation solution H; fermentation temperature 20-30°C, fermentation initial pH 5-6, fermentation ventilation 5-7L / min, fermentation time 18h, fermentation stirring speed, first 4h 210r / min, last 14h 70r / min; The fungus species in S4 are red-rooted bamboo fungus and Dinghulin Agaricus; The plant extract fermentation product is a mixture of mycelium E and fermentation liquid H; Said: Centella asiatica extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.; Sea buckthorn fruit extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.; Rosemary extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.; Lily extract: purchased from Shaanxi Baichuan Biotechnology Co., Ltd.

2. The composition according to claim 1, characterized in that The plant extract fermentation product comprises the following raw materials in parts by weight: 1-2 parts of Centella asiatica extract; 2-5 parts of sea buckthorn fruit extract; Rosemary extract 0.2-0.7 parts; 0.1-0.5 parts of lily extract.

3. The composition according to claim 1, characterized in that The mass fraction ratio of the peanut shell powder, fish maw powder and corn pollen is 1:0.1-0.4:3-5, and the peanut shell powder, fish maw powder and corn pollen account for 15% of the mass percentage of the mixed solution A.

4. A light pink soothing repair lotion, characterized in that: The invention comprises the reddish soothing repairing composition according to any one of claims 1 to 3.

5. The repairing lotion according to claim 4, characterized in that: The preparation method of the repairing lotion is as follows: Take 1-3% honey, 0.1-0.3% betaine and 1-4% milk extract by weight and add them into a container, heat to 40-60°C, stir evenly, wait until the temperature drops to ≤40°C, add 0.5-5% light red soothing repair composition, homogenize and sterilize.

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