Microfluidic chip and clamping clamp for microfluidic chip

Abstract

The application relates to a micro-fluidic chip and a clamping clamp for the micro-fluidic chip, and relates to a micro-fluidic chip structure. The application aims to solve the problems of complex operation clamp structure, complex debugging and operation, limited manufacturing precision, easy liquid leakage, fluid mixing, influence on the accuracy and reliability of experimental results, frequent maintenance and replacement, increased experimental cost and operation period of the traditional micro-fluidic chip. The application comprises a set of transparent plates and a film with flow channels, one film with flow channels is sealingly arranged between the upper transparent plate and the lower transparent plate, and the flow channels of the film with flow channels are in communication with the liquid flow-through holes of the upper transparent plate. The micro-fluidic chip is arranged between the upper chip clamping plate and the lower chip clamping plate, one end of the fixed clamping plate is hingedly arranged with one end of the turnover clamping plate, and the other end of the turnover clamping plate is locked on the other end of the fixed clamping plate through a lock tongue. The application is applied to the technical field of micro-fluidic chips.

Description

TECHNICAL FIELD

[0001] The present application relates to a microfluidic chip structure, in particular to a microfluidic chip and a clamping clamp for the microfluidic chip, and belongs to the technical field of microfluidic chips. BACKGROUND

[0002] Since the 1990s, microfluidic technology has gradually become an important tool in the field of cell research due to its numerous advantages. As a highly integrated biological analysis platform, microfluidic chips have wide application prospects in medical diagnosis, biochemical research, environmental monitoring and other fields. However, there are some challenges in the operation process of microfluidic chips, one of which is the design and performance of the operation clamp.

[0003] The traditional microfluidic chip operation clamp has the following shortcomings:

[0004] Complexity and tediousness: the traditional clamp structure is complex, requiring complex debugging and operation procedures.

[0005] Precision and reliability: due to the limited manufacturing precision of the traditional clamp, it is easy to cause liquid leakage, fluid mixing and other problems, affecting the accuracy and reliability of the experimental results.

[0006] Cost and sustainability: the traditional clamp is expensive to manufacture and needs to be frequently maintained and replaced, increasing the experimental cost and operation cycle.

[0007] In the face of these challenges, there is an urgent need to seek a new type of microfluidic chip operation clamp, which should provide a simpler, more accurate and more economical operation scheme, so as to promote the further development and application of microfluidic chip technology.

[0008] Meet the needs of efficient, reliable and economic experiments at the same time, and promote the development of the application range and depth of microfluidic chip technology. SUMMARY

[0009] The purpose of the present application is to solve the problems of the traditional microfluidic chip operation clamp, such as complex structure, complex debugging and operation, limited manufacturing precision, easy to cause liquid leakage, fluid mixing, affecting the accuracy and reliability of the experimental results, and the need for frequent maintenance and replacement, increasing the experimental cost and operation cycle, and to provide a microfluidic chip and a clamping clamp for the microfluidic chip.

[0010] The technical solution adopted by the present application to solve the above problems is:

[0011] A micro-fluidic chip, which comprises a set of transparent plates and a film with flow channels, the set of transparent plates comprises an upper transparent plate and a lower transparent plate, at least one liquid inflow hole and at least one liquid outflow hole are formed at both ends of the upper transparent plate respectively, a film with flow channels is arranged between the upper transparent plate and the lower transparent plate, the upper transparent plate and the lower transparent plate are arranged correspondingly, and the flow channels of the film with flow channels are arranged in communication with the liquid flow holes of the upper transparent plate.

[0012] Further, the upper transparent plate and the lower transparent plate are both rectangular plate bodies, and the upper transparent plate and the lower transparent plate are both made of materials with good light transmission.

[0013] A clamping fixture for a micro-fluidic chip, which comprises a fixed clamping plate, a turnover clamping plate, a lock tongue, an upper chip clamping plate and a lower chip clamping plate;

[0014] The upper chip clamping plate is installed above the lower chip clamping plate, at least one micro-fluidic chip is installed between the upper chip clamping plate and the lower chip clamping plate, one end of the fixed clamping plate is hingedly connected to one end of the turnover clamping plate, the upper chip clamping plate and the lower chip clamping plate are installed between the fixed clamping plate and the turnover clamping plate, and the other end of the turnover clamping plate is locked on the other end of the fixed clamping plate through the lock tongue.

[0015] Further, the fixed clamping plate is a rectangular plate body, the fixed clamping plate is provided with a lower clamping plate limiting hole, the turnover clamping plate is a rectangular plate body, the turnover clamping plate is provided with an upper clamping plate limiting hole, the lower chip clamping plate is clamped in the lower clamping plate limiting hole of the fixed clamping plate, the upper chip clamping plate is clamped in the upper clamping plate limiting hole of the turnover clamping plate, and the upper chip clamping plate and the lower chip clamping plate are arranged correspondingly.

[0016] Further, at least one liquid flow hole is formed at each end of the micro-fluidic chip clamped on the upper chip clamping plate, and each liquid inflow hole and each liquid outflow hole of the micro-fluidic chip are arranged correspondingly with one liquid flow hole on the upper chip clamping plate above.

[0017] Further, steps are formed on both sides of the lower chip clamping plate, clamping grooves for clamping the lower chip clamping plate are formed on both sides of the lower clamping plate limiting hole of the fixed clamping plate, the steps on both sides of the lower chip clamping plate are clamped in the clamping grooves on both sides of the lower clamping plate limiting hole of the fixed clamping plate, and the steps on each side of the lower chip clamping plate are fixedly installed on the groove bottom of the clamping groove in the lower clamping plate limiting hole through bolts.

[0018] Steps are formed on both sides of the upper chip clamping plate, clamping grooves for clamping the upper chip clamping plate are formed on both sides of the upper clamping plate limiting hole of the turnover clamping plate, the steps on both sides of the upper chip clamping plate are clamped in the clamping grooves on both sides of the upper clamping plate limiting hole of the turnover clamping plate, and the steps on each side of the upper chip clamping plate are fixedly installed on the groove bottom of the clamping groove in the upper clamping plate limiting hole through bolts.

[0019] Further, it also includes a locking mechanism cover plate, the lock tongue is installed in the groove of one end of the fixed clamping plate, the protruding end of the lock tongue is inserted into the locking slot of the turnover clamping plate through the side wall of one end of the fixed clamping plate, and the turnover clamping plate is adhered and locked on the upper end surface of the fixed clamping plate through the protruding end of the lock tongue, and the locking end of the locking mechanism cover plate is locked and fixedly installed on the fixed end of the lock tongue in the groove of the fixed clamping plate.

[0020] Further, the protruding piece is provided at one end of the dial piece and is fixed with the dial piece, the locking mechanism cover plate is provided at the other end of the dial piece, and at least one elastic pressing piece is arranged between the protruding piece and the locking mechanism cover plate; the protruding piece is inserted into the locking slot of the turnover clamping plate through the elastic pressing piece, and the turnover clamping plate is locked.

[0021] Further, it also includes two silica gel pads and a plurality of hollow rubber plugs, two silica gel pads are arranged between each microfluidic chip and the chip clamping plate, a hollow rubber plug is sealingly arranged in the liquid flow hole of the chip clamping plate, and an inlet tube is arranged in each hollow rubber plug, the upper end of the inlet tube is connected with the injection device through the liquid flow hole of the chip clamping plate, and the lower end of the inlet tube is connected with the microfluidic chip under the wrapping of the hollow rubber plug, so that the liquid in the microfluidic chip is input into the microfluidic chip.

[0022] Further, it also includes an optical axis and two shaft sleeves; one end of the fixed clamping plate and one end of the turnover clamping plate are hingedly arranged through the optical axis and the two shaft sleeves, so that the turnover clamping plate is turned on the fixed clamping plate around the optical axis.

[0023] The beneficial effects of the present application are as follows:

[0024] 1. The clamping clamp of the present application is convenient for opening and closing to take and place the chip, the outer structure of the clamping clamp is composed of a fixed clamping plate 1 and a turnover clamping plate 7, the turnover clamping plate 7 is hingedly connected with the fixed clamping plate 1 through an optical axis 9 and a shaft sleeve 8, and a locking mechanism cover plate

[0025] 2. The lock tongue 3 constitutes a locking structure, so that the microfluidic chip clamp is quickly opened and closed and fastened, and the inner structure of the microfluidic chip clamp, the microfluidic chip is convenient for disassembly, replacement, disinfection and other operations.

[0026] 2. The microfluidic chip and the clamping clamp of the microfluidic chip of the present application are small in size and can be placed instead of a hole plate, and are simple in structure and convenient for disinfection treatment; the clamping clamp of the microfluidic chip can clamp multiple microfluidic chips at the same time during the experiment, and can replace the upper and lower clamping plates of the chip to adapt to different sizes and different numbers of chips. The microfluidic chip and the clamping clamp of the microfluidic chip of the present application are simple in structure and convenient for manufacturing, and cost is saved.

[0027] 3、The microfluidic chip clamp of the application has a fast and firm multi-chip sealing connection mode. When the microfluidic chip is unlocked, the turnover clamp plate 7 can be lifted and turned over to the fixed clamp plate 1. A plurality of different types of microfluidic chips can be filled between the turnover clamp plate 7 and the fixed clamp plate 1, and the thickness-matched rubber plug and rubber strip are installed on all chips at the same time, and inserted into the liquid inlet pipe. After the turnover clamp plate 7 is lowered and locked through the fixed clamp plate 1, the chip upper clamp plate 10, the rubber strip, the microfluidic chip and the chip lower clamp plate 4 are pressed at the same time, so that the flow channels or chambers of the plurality of microfluidic chips are sealed at the same time, preventing liquid from leaking out of both sides of each microfluidic chip during use. At the same time, the upper transparent plate of the microfluidic chip and the chip upper clamp plate 10 clamp the hollow silica gel plug 10-1, so that the input and output areas of the plurality of microfluidic chips are sealed at the same time, preventing liquid from leaking into the input and output areas of each microfluidic chip during liquid inlet.

[0028] 4、The microfluidic chip clamp has a convenient and flexible instant plug-in design for multiple microfluidic chips. The structure that remains unchanged during the experiment of multiple microfluidic chips is separated from the structure that needs to be adjusted in real time. The structure that remains unchanged constitutes the outer structure of the microfluidic chip clamp, including the opening and closing structure, the locking structure, etc. The structure that needs to be adjusted in real time constitutes the inner structure of the microfluidic chip clamp, including the number and layout adjustable microfluidic chip unit. The inner structure of the microfluidic chip clamp is fixed by a countersunk screw, which is easy to disassemble. When high-temperature sterilization and other disinfection processes are carried out, only the inner structure of the microfluidic chip clamp needs to be treated, reducing the wear of the outer structure of the microfluidic chip clamp and improving the service life of the clamp. At the same time, when different experiments are carried out, only the inner structure of the clamp with different number and layout of microfluidic chip units needs to be replaced, saving cost and facilitating manufacturing. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 It is a schematic diagram of the overall structure of a microfluidic chip clamping clamp of the application.

[0030] Figure 2 It is an exploded view of a microfluidic chip clamping clamp before assembly.

[0031] Figure 3 It is a schematic diagram of a microfluidic chip structure.

[0032] Figure 4 It is a schematic diagram of a microfluidic chip installed between the chip upper clamp plate 10 and the chip lower clamp plate 4.

[0033] Figure 5 It is a schematic diagram of the locking tongue 3 locked in the groove of the turnover clamp plate 7. DETAILED DESCRIPTION

[0034] Specific implementation one: combined with Figure 3The microfluidic chip of the embodiment comprises a set of transparent plates 5 and a film 6 with flow channels. The set of transparent plates 5 comprises an upper transparent plate and a lower transparent plate. The upper transparent plate has at least one liquid inflow through hole and at least one liquid outflow through hole at both ends, respectively. A film 6 with flow channels is sealingly arranged between the upper transparent plate and the lower transparent plate. The upper transparent plate and the lower transparent plate are correspondingly arranged, and the flow channels of the film 6 with flow channels are in communication with the liquid flow through holes of the upper transparent plate.

[0035] The microfluidic chip of the embodiment comprises a three-layer structure, i.e., an upper transparent plate, a film 6 with flow channels, and a lower transparent plate. The upper transparent plate is made of a material with good light transmission, including but not limited to glass and other materials. The film 6 with flow channels is made of a film including but not limited to PDMS and other materials. The flow channels or chambers are processed by photolithography, film cutting, and other methods. The input position matches the position of the through hole on the upper layer of the chip. The lower transparent plate is made of a transparent material including but not limited to glass and other materials. The lower transparent plate can be observed by an inverted microscope or made of an opaque material including but not limited to silicon and observed by a normal microscope.

[0036] Specific implementation method two: combination Figure 3 The microfluidic chip of the embodiment comprises an upper transparent plate and a lower transparent plate, both of which are rectangular plates and made of materials with good light transmission. Other methods are the same as those of the first specific implementation method.

[0037] Specific implementation method three: combination Figures 1-5 The microfluidic chip of the embodiment comprises a fixed clamping plate 1, a turnover clamping plate 7, a lock tongue 3, a chip upper clamping plate 10, and a chip lower clamping plate 4.

[0038] The chip upper clamping plate 10 is installed above the chip lower clamping plate 4. At least one microfluidic chip is installed between the chip upper clamping plate 10 and the chip lower clamping plate 4. One end of the fixed clamping plate 1 is hingedly connected to one end of the turnover clamping plate 7. The chip upper clamping plate 10 and the chip lower clamping plate 4 are installed between the fixed clamping plate 1 and the turnover clamping plate 7. The other end of the turnover clamping plate 7 is locked on the other end of the fixed clamping plate 1 by the lock tongue 3.

[0039] The other end of the turnover clamping plate 7 is provided with a lock tongue card mounting groove. The lock tongue mounting groove is adapted to the extension end of the lock tongue 3. The lock tongue 3 is positioned on the fixed clamping plate 1 by the lock mechanism cover plate 2, and the turnover clamping plate 7 is locked on the fixed clamping plate 1 by the lock tongue 3. The fixed clamping plate 1 and the turnover clamping plate 7 are seamlessly attached. The number of microfluidic chips installed between the chip upper clamping plate 10 and the chip lower clamping plate 4 is selected according to requirements.

[0040] Specific implementation method four: combinationFigure 1 、 Figure 2 and Figure 4 The embodiment is described, and the clamping fixture of the microfluidic chip in the embodiment is characterized in that the fixed clamping plate 1 is a rectangular plate body, the fixed clamping plate 1 is processed with a lower clamping plate limiting hole, the turnover clamping plate 7 is a rectangular plate body, the turnover clamping plate 7 is processed with an upper clamping plate limiting hole, the chip lower clamping plate 4 is clamped in the lower clamping plate limiting hole of the fixed clamping plate 1, the chip upper clamping plate 10 is clamped in the upper clamping plate limiting hole of the turnover clamping plate 7, and the chip upper clamping plate 10 and the chip lower clamping plate 4 are correspondingly arranged. The upper clamping plate limiting hole on the turnover clamping plate 7 and the lower clamping plate limiting hole of the fixed clamping plate 1 are both round-cornered rectangular holes, and other methods are the same as those in the third embodiment.

[0041] The lower clamping plate limiting hole of the fixed clamping plate 1 and the upper clamping plate limiting hole of the turnover clamping plate 7 are rectangular holes, which are used for compatible observation of upright and inverted microscopes, the area of the rectangular hole covers the internal flow channel of the microfluidic chip as much as possible, sufficient observation area is provided, and no dead angle observation under the microscope is realized; the upper clamping plate limiting hole of the turnover clamping plate 7 is used for placing the microfluidic chip, and sufficient space is left in the middle of the side wall to facilitate the taking and placing of the microfluidic chip.

[0042] The fifth embodiment is described in combination with Figure 1 、 Figure 2 and Figure 4 The embodiment is described, and the clamping fixture of the microfluidic chip in the embodiment is characterized in that at least one liquid flow-through hole is processed on each end of the clamped microfluidic chip on the chip upper clamping plate 10, and each liquid inflow hole and liquid outflow hole of the microfluidic chip is correspondingly arranged with one liquid flow-through hole on the chip upper clamping plate 10 above. Other methods are the same as those in the first embodiment.

[0043] In the embodiment, the number of the liquid flow-through holes on the two ends of the clamped microfluidic chip on the chip upper clamping plate 10 is selected according to requirements.

[0044] The sixth embodiment is described in combination with Figure 1 、 Figure 2 The embodiment is described, and the clamping fixture of the microfluidic chip in the embodiment is characterized in that steps are respectively processed on the two sides of the chip lower clamping plate 4, the lower clamping plate limiting hole on the fixed clamping plate 1 is processed with clamping grooves for clamping the chip lower clamping plate 4, the steps on the two sides of the chip lower clamping plate 4 are clamped on the clamping grooves on the two sides of the lower clamping plate limiting hole on the fixed clamping plate 1, and the steps on each side of the chip lower clamping plate 4 are fixedly installed on the groove bottom of the clamping groove in the lower clamping plate limiting hole by bolts,

[0045] The chip-on-clamp plate 10 is provided with a step on each side, the upper clamp plate limiting hole of the turnover clamp plate 7 is provided with a clamping groove for clamping the chip-on-clamp plate 10 on each side, the step on each side of the chip-on-clamp plate 10 is clamped in the clamping groove on each side of the upper clamp plate limiting hole of the turnover clamp plate 7, and the step on each side of the chip-on-clamp plate 10 is fixedly installed on the groove bottom of the clamping groove of the upper clamp plate limiting hole by a bolt. The upper clamp plate limiting hole of the turnover clamp plate 7, the lower clamp plate limiting hole of the fixed clamp plate 1, the clamping groove on each side of the lower clamp plate limiting hole of the fixed clamp plate 1 and the clamping groove on each side of the upper clamp plate limiting hole of the turnover clamp plate 7 are all used for limiting, and other methods are the same as those in the second or fifth embodiment.

[0046] The seventh embodiment is combined with the first embodiment. Figures 1-5 In the embodiment, the microfluidic chip clamping jig further comprises a locking mechanism cover plate 2, a locking tongue 3 is installed in the groove at one end of the fixed clamp plate 1, the protruding end of the locking tongue 3 is inserted into the locking groove of the turnover clamp plate 7 through the side wall at one end of the fixed clamp plate 1, and the turnover clamp plate 7 is attached and locked on the upper end face of the fixed clamp plate 1 through the protruding end of the locking tongue 3, and the locking end of the locking mechanism cover plate 2 is fixedly installed on the fixed end of the locking tongue 3 installed in the groove of the fixed clamp plate 1. Other methods are the same as those in the sixth embodiment.

[0047] The eighth embodiment is combined with the first embodiment. Figure 1 And Figure 5 In the embodiment, the microfluidic chip clamping jig further comprises a locking mechanism cover plate 2, a locking tongue 3 is installed in the groove at one end of the fixed clamp plate 1, the protruding end of the locking tongue 3 is inserted into the locking groove of the turnover clamp plate 7 through the side wall at one end of the fixed clamp plate 1, and the turnover clamp plate 7 is attached and locked on the upper end face of the fixed clamp plate 1 through the protruding end of the locking tongue 3, and the locking end of the locking mechanism cover plate 2 is fixedly installed on the fixed end of the locking tongue 3 installed in the groove of the fixed clamp plate 1. Other methods are the same as those in the sixth embodiment.

[0048] In the embodiment, the locking tongue 3 is provided with a knob above, the knob is moved to compress the spring, the locking tongue 3 is withdrawn from the groove, and the microfluidic chip clamping jig is unlocked, at which time the turnover clamp plate 7 can be lifted.

[0049] The ninth embodiment is combined with the first embodiment. Figures 1-4To illustrate the embodiment, the microfluidic chip clamping jig described in the embodiment further comprises two silica gel pads 10-2 and a plurality of hollow rubber plugs 10-1, two silica gel pads 10-2 are arranged between each microfluidic chip and the chip upper clamping plate 10, a hollow rubber plug 10-1 is sealingly arranged in the liquid flow hole of the chip upper clamping plate 10, and a liquid inlet pipe is arranged in each hollow rubber plug 10-1, the upper end of the liquid inlet pipe penetrates through the liquid flow hole of the chip upper clamping plate 10 and is connected with the injection device, and the lower end of the liquid inlet pipe is connected with the microfluidic chip under the wrapping of the hollow rubber plug 10-1, so as to realize the input of liquid in the microfluidic chip. The other methods are the same as those in the eighth embodiment.

[0050] In the embodiment, the chip upper clamping plate 10 and the chip lower clamping plate 4 are provided with silica gel pads 10-2, the thickness of the silica gel pads 10-2 satisfies the clamping of the microfluidic chip clamp, and the liquid input hole of the chip upper clamping plate 10 is connected with the microfluidic chip through the hollow rubber plug 10-1, the upper part of the hollow rubber plug 10-1 penetrates into the liquid input hole of the chip upper clamping plate 10, and the lower part of the hollow rubber plug 10-1 needs to have the same thickness as that of the silica gel pad 10-2, so as to avoid uneven stress and cause the cracking of the microfluidic chip. The liquid inlet pipe is inserted into the hollow rubber plug 10-1, the upper end of the liquid inlet pipe penetrates through the liquid input hole of the chip upper clamping plate 10 and is connected with the liquid driving device including but not limited to the injection pump, and the lower end of the liquid inlet pipe is connected with the microfluidic chip under the wrapping of the hollow rubber plug 10-1, so as to realize the input and output of liquid in the microfluidic chip.

[0051] The input and output ends of each microfluidic chip unit of the microfluidic chip clamp have a plurality of rubber pipe connection lines, and can realize multi-chip joint experiments, including but not limited to the following modes: multi-chip parallel connection, all chip input ends are connected with the liquid driving device, and each chip is independently experimented; multi-chip series connection, the input end of one chip is connected with the liquid driving device, the output end is connected with the input end of the next chip, all chips are connected in sequence, each chip has an observation window, so that the reaction in the chip can be observed and recorded step by step; the parallel and series connection modes can be used together to construct a multi-chip complex hierarchical conduction network.

[0052] The tenth embodiment is combined with Figure 1 , Figure 2 To illustrate the embodiment, the microfluidic chip clamping jig described in the embodiment further comprises an optical axis 9 and two shaft sleeves 8; one end of the fixed clamping plate 1 and one end of the turnover clamping plate 7 are hingedly arranged through the optical axis 9 and the two shaft sleeves 8, so as to realize the turnover of the turnover clamping plate 7 on the fixed clamping plate 1 around the optical axis 9. The other methods are the same as those in the eighth embodiment.

[0053] The above specific embodiments can be partially adjusted in different ways by those skilled in the art without departing from the principles and purposes of the present application, the protection scope of the present application is subject to the claims and is not limited by the above specific embodiments, each implementation within the scope is subject to the present application.

Claims

1. A microfluidic chip clamping fixture, the microfluidic chip comprising a set of transparent plates (5) and a film (6) with flow channels, the set of transparent plates (5) comprising an upper transparent plate and a lower transparent plate, both ends of the upper transparent plate being respectively processed with at least one liquid inflow through hole and at least one liquid outflow through hole, one film (6) with flow channels being sealingly arranged between the upper transparent plate and the lower transparent plate, the upper transparent plate and the lower transparent plate being correspondingly arranged, and the flow channels of the film (6) with flow channels being arranged in communication with the liquid flow through holes of the upper transparent plate, the upper transparent plate and the lower transparent plate are both rectangular plate bodies, and the upper transparent plate and the lower transparent plate are both made of materials with good light transmission, the clamping fixture comprising a fixed clamping plate (1), a turnover clamping plate (7), a lock tongue (3), an upper chip clamping plate (10) and a lower chip clamping plate (4); characterized in that: The clamping clamp further comprises a locking mechanism cover plate (2), the locking tongue (3) comprises a protruding piece (3-1), a push piece (3-2) and at least one elastic pressing piece (3-3); The chip upper clamping plate (10) is arranged above the chip lower clamping plate (4), at least one microfluidic chip is arranged between the chip upper clamping plate (10) and the chip lower clamping plate (4), one end of the fixed clamping plate (1) is hingedly arranged with one end of the turnover clamping plate (7), the chip upper clamping plate (10) and the chip lower clamping plate (4) are arranged between the fixed clamping plate (1) and the turnover clamping plate (7), the other end of the turnover clamping plate (7) is locked on the other end of the fixed clamping plate (1) through the locking tongue (3), the locking tongue (3) is arranged in the groove at one end of the fixed clamping plate (1), the protruding end of the locking tongue (3) is inserted into the locking groove of the turnover clamping plate (7) through the side wall of one end of the fixed clamping plate (1), and the turnover clamping plate (7) is attached and locked on the upper end surface of the fixed clamping plate (1) through the protruding end of the locking tongue (3), the locking end of the locking mechanism cover plate (2) is fixedly arranged on the fixed end of the locking tongue (3) arranged in the groove of the fixed clamping plate (1), The protruding piece (3-1) is arranged at one end of the push piece (3-2) and fixed with the push piece (3-2), the locking mechanism cover plate (2) is arranged at the other end of the push piece (3-2), and the at least one elastic pressing piece (3-3) is arranged between the protruding piece (3-1) and the locking mechanism cover plate (2), the protruding piece (3-1) is inserted into the locking groove of the turnover clamping plate (7) through the elastic pressing piece (3-3) and locks the turnover clamping plate (7).

2. The clamping fixture of a microfluidic chip according to claim 1, wherein: The fixed clamping plate (1) is a rectangular plate body, the fixed clamping plate (1) is provided with a lower clamping plate limiting hole, the turnover clamping plate (7) is a rectangular plate body, the turnover clamping plate (7) is provided with an upper clamping plate limiting hole, the chip lower clamping plate (4) is clamped in the lower clamping plate limiting hole of the fixed clamping plate (1), the chip upper clamping plate (10) is clamped in the upper clamping plate limiting hole of the turnover clamping plate (7), and the chip upper clamping plate (10) and the chip lower clamping plate (4) are correspondingly arranged.

3. The clamping fixture of a microfluidic chip according to claim 2, wherein: At least one liquid flow hole is arranged at each end of the microfluidic chip clamped on the chip upper clamping plate (10), and each liquid inflow hole and liquid outflow hole of the microfluidic chip is correspondingly arranged with a liquid flow hole on the chip upper clamping plate (10) above.

4. The clamping fixture of claim 2, wherein: The chip lower clamping plate (4) is provided with steps on both sides, the lower clamping plate limiting hole of the fixed clamping plate (1) is provided with clamping grooves for clamping the chip lower clamping plate (4) on both sides, the steps on both sides of the chip lower clamping plate (4) are clamped in the clamping grooves on both sides of the lower clamping plate limiting hole of the fixed clamping plate (1), and the steps on each side of the chip lower clamping plate (4) are fixedly arranged on the groove bottoms of the clamping grooves in the lower clamping plate limiting hole by bolts, The chip upper clamping plate (10) is provided with steps on both sides, the upper clamping plate limiting hole of the turnover clamping plate (7) is provided with clamping grooves for clamping the chip upper clamping plate (10) on both sides, the steps on both sides of the chip upper clamping plate (10) are clamped in the clamping grooves on both sides of the upper clamping plate limiting hole of the turnover clamping plate (7), and the steps on each side of the chip upper clamping plate (10) are fixedly arranged on the groove bottoms of the clamping grooves in the upper clamping plate limiting hole by bolts.

5. The clamping fixture of a microfluidic chip according to claim 4, wherein: It further comprises two silica gel pads (10-2) and a plurality of hollow rubber plugs (10-1), two silica gel pads (10-2) are arranged between each micro-fluidic chip and the chip-on-clamp (10), a hollow rubber plug (10-1) is sealingly arranged in the liquid flow-through hole of the chip-on-clamp (10), and one liquid inlet pipe is arranged in each hollow rubber plug (10-1), the upper end of the liquid inlet pipe is connected with the injection device through the liquid flow-through hole of the chip-on-clamp (10), and the lower end of the liquid inlet pipe is connected with the micro-fluidic chip under the wrapping of the hollow rubber plug (10-1), so that the liquid in the micro-fluidic chip is input into the micro-fluidic chip.

6. The clamping fixture of a microfluidic chip according to claim 1, wherein: It further comprises an optical axis (9) and two shaft sleeves (8); one end of the fixed clamp plate (1) and one end of the turnover clamp plate (7) are hingedly arranged through the optical axis (9) and the two shaft sleeves (8), so that the turnover clamp plate (7) is turned over on the fixed clamp plate (1) around the optical axis (9).

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