A kind of Trichoderma acanthosporum culture device and culture method

Abstract

The invention relates to the technical field of strain culture, in particular to a Trichoderma spinulosa strain culture device and a culture method, comprising a culture box and a heating system; three box doors are respectively hinged on an upper opening, a middle opening and a lower opening; an upper support plate is installed in the culture box, a plurality of small culture trays are placed on the upper support plate, a dividing component is installed above the upper support plate, a seed separation mechanism is installed at the upper part of the culture box, a lifting mechanism is installed at the middle and lower parts of the culture box, the lifting mechanism is used to lift a large culture tray, the seed separation mechanism is used to evenly distribute small pieces of culture medium in the small culture trays to the large culture tray located in the middle of a chamber of the culture box, a middle support plate is rotatably installed on the middle inner wall of the culture box, a plurality of lamp beads are arranged in a matrix on the middle support plate, the seed separation mechanism comprises a crossbeam, the crossbeam moves above the large culture tray, and a plurality of light sensing elements are arranged in a matrix at the bottom of the crossbeam; the device can automatically and accurately intervene according to the development of the strains, so that the strains develop evenly and the strain culture efficiency is improved.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial strain cultivation, and in particular to a Trichoderma acanthosporium bacterial strain cultivation device and a cultivation method. Background Art

[0002] Trichoderma aculeatus belongs to the Deuteromycetes, Hyphomycetales, and Trichoderma genus. It is a Trichoderma with aculeatus. As an important microbial resource, Trichoderma aculeatus has broad application prospects and huge development potential in biological control, promoting plant growth, soil improvement, and industrial applications.

[0003] The prior art discloses a variety of methods for culturing Trichoderma aspergillus strains. For example, a Chinese invention patent with publication number CN107142213B proposes a strain of Trichoderma aspergillus with a growth-promoting effect, a cultivation method and an application thereof. The cultivation method proposed in the patent involves strain activation, shake flask seed preparation, primary liquid seed cultivation, and then solid fermentation. The fermentation conditions are suitable, and the production cycle of a batch of Trichoderma aspergillus fermentation products is about 9 to 12 days, which can effectively shorten the production cycle and greatly improve the production efficiency.

[0004] The prior art also discloses a variety of bacterial culture devices, for example, a Chinese invention patent with publication number CN116162524B proposes a Trichoderma fermentation production device, which has the function of realizing the intermittent quantitative liquid addition operation of Trichoderma culture in the fermentation kettle body, improving the efficiency of the Trichoderma layered culture process, avoiding the deposition of culture solution caused by adding too much culture solution at one time during the culture process, affecting the absorption effect of Trichoderma on the culture solution, and utilizing the intermittent quantitative liquid addition method to promote Trichoderma to form multiple layers, thereby improving the overall culture efficiency of Trichoderma.

[0005] However, when the above-mentioned Trichoderma culture device is working, the strains are directly cultured in a larger culture dish, and more strains need to be invested in the early stage of culture. Moreover, since the temperature of the culture dish cannot be locally controlled during the culture of the strains, intervention cannot be performed when the strains are not fully developed, which easily causes uneven distribution of strain colonies in the culture dish, resulting in reduced strain culture efficiency. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a Trichoderma spinulosa strain culture device and culture method which can automatically and accurately intervene according to the development of the strain, make the strain develop evenly, and improve the strain culture efficiency.

[0007] The invention discloses a Trichoderma spinulosa culture device, comprising an incubator and a heating system, wherein a chamber is arranged inside the incubator, the heating system is installed in the chamber of the incubator, and the heating system comprises a heater and a plurality of temperature sensors; the device also comprises three doors, an upper support plate, a plurality of small culture trays, a plurality of large culture trays, a middle support plate, a plurality of lamp beads, a plurality of light sensing elements of a cross beam, a plurality of light sensing elements, a vibration plate, a plurality of springs and a vibration motor, wherein an upper opening, a middle opening and a lower opening are arranged on the front end surface of the incubator from top to bottom, the three doors are hinged on the upper opening, the middle opening and the lower opening respectively, the upper support plate is installed in the chamber of the incubator, the upper support plate is flush with the upper opening, the upper support plate holds a plurality of small culture trays, a splitting component is installed above the upper support plate, the splitting component is used for evenly dividing culture medium in the small culture trays, the vibration plate is elastically installed at the lower part of the chamber of the incubator through a plurality of springs, the vibration plate is flush with the lower opening of the incubator, a vibration motor is installed at the bottom of the vibration plate, the vibration plate holds a plurality of large culture trays, and the bottom of the large culture trays is transparent;

[0008] A seeding mechanism is installed at the upper part of the chamber of the incubator, and a lifting mechanism is installed at the middle and lower part of the chamber of the incubator. The lifting mechanism is used to lift multiple large culture trays from the lower part of the chamber of the incubator to the middle part of the chamber. The seeding mechanism is used to evenly distribute small pieces of culture medium in the small culture trays to the large culture trays located in the middle part of the chamber of the incubator. The middle support plate is rotatably installed on the middle inner wall of the incubator, and a plurality of lamp beads are arranged in a matrix on the middle support plate. The plurality of lamp beads face the bottom of the large culture tray located in the middle part of the chamber of the incubator. The seeding mechanism includes a crossbeam, which moves above the large culture tray, and a plurality of light sensing elements are arranged in a matrix at the bottom of the crossbeam.

[0009] When working, the heating system heats the air in the chamber of the incubator, and detects the temperature values ​​at various locations through multiple temperature sensors. The heating system is adjusted according to the temperature values, so as to accurately control the temperature in the chamber of the incubator. When the temperature reaches the required temperature, the upper opening of the box door is opened, and a small culture plate containing an inoculated gel culture medium is placed on the upper support plate, so that a small amount of bacteria is cultured in the small culture plate at the first level. The lower opening of the box door is opened, and various raw materials of the culture medium are added to the large culture plate. The large culture plate is placed on the vibration plate through the lower opening of the incubator, and the vibration motor starts to vibrate. The vibration plate is elastically supported by multiple springs, so that the vibration plate vibrates the large culture tray, so that the raw materials in the large culture tray are fully mixed to form a culture medium, thereby improving practicality and allowing the large culture tray to be preheated and pretreated. When the culture medium in the small culture tray is full of bacterial strains, the dividing component evenly divides the culture medium in the small culture tray into multiple small pieces, and the lifting mechanism raises the large culture tray to the middle of the chamber of the incubator. The middle support plate rotates to a horizontal position so that multiple lamp beads are aligned with the bottom of the large culture tray. The seeding mechanism moves the small culture tray to the top of the large culture tray, and the seeding mechanism divides the culture medium in the small culture tray into multiple small pieces. The base and small pieces of bacterial strains are evenly distributed in the large culture dish, so that the bacterial strains undergo secondary culture and development in the culture medium of the large culture dish, and multiple interventions are performed during the bacterial strain cultivation process in the large culture dish, and the interventions are: multiple lamp beads are turned on to illuminate the bottom of the large culture dish, so that the multiple lamp beads emit light that penetrates the large culture dish, the culture medium and the bacterial colony to illuminate multiple light sensing elements, and the crossbeam moves above the large culture dish. The multiple light sensing elements sense the light and detect the light transmittance of the large culture dish and locate the positions where the light transmittance is too strong and too weak. The bacterial strains are poorly developed at the positions where the light transmittance is too strong, and the control of the multiple lamp beads The device strengthens the illumination of the multiple lamp beads at the position where the light transmission is too strong, and reduces the illumination of the multiple lamp beads at the position where the light transmission is too weak, so that the temperature of the culture medium at the position where the light transmission is too strong in the large culture dish is appropriately increased, and the temperature of the culture medium at the position where the light transmission is too weak in the large culture dish is appropriately reduced, thereby controlling the temperature of the local part of the culture dish, and allowing the multiple lamp beads to automatically and accurately intervene according to the development of the bacterial strains in the large culture dish. In order to improve the heating intervention effect of the lamp beads, the lamp beads can use full-spectrum lamp beads or corresponding spectrum lamp beads, so that the bacterial colonies in the large culture dish can develop and distribute evenly in the later stage of culture, thereby improving the efficiency of bacterial strain culture.

[0010] Preferably, the dividing component includes a push cylinder and a blade group, the fixed end of the push cylinder is installed on the top wall of the incubator, the blade group is installed on the lower end of the piston rod of the push cylinder, the blade group is located above the upper support plate, the blade group is composed of a plurality of horizontal blades and a plurality of vertical blades staggered to form a plurality of squares, and the blade group matches the small culture plate; the small culture plate is filled with gel or solid culture medium, the culture medium is inoculated with bacterial strains, the small culture plate is placed on the upper support plate, so that the bacterial strains in the small culture plate are subjected to primary culture, when the bacterial strains in the small culture plate are fully developed and produce a large number of spore bacterial strains, the piston rod of the push cylinder is extended downward to allow the blade group to extend into the small culture plate to evenly divide the culture medium into a plurality of small pieces, to prevent the small pieces of culture medium from being stuck in the blade group, a lubricant can be pre-coated on the blades of the blade group, or a non-stick coating can be provided on the surface of the blade group, the structure is simple and the practicality is good.

[0011] Preferably, the seeding mechanism includes a moving component, a clamping component and a pick-and-place component, the moving component is installed on the upper part of the chamber of the incubator, the moving component drives the crossbeam to move horizontally, the pick-and-place component is installed on the crossbeam, the pick-and-place component moves longitudinally along the crossbeam, the clamping component is installed on the pick-and-place component, the clamping component is used to clamp the small culture plate, and the pick-and-place component is used to take out small pieces of culture medium from the small culture plate and put them into the large culture plate, the pick-and-place component includes a horizontal slide, a vertical slide, an electromagnetic coil, a magnet, a frame, a push cylinder 2 and a suction cup, the horizontal slide is slidably installed on the crossbeam, the horizontal slide is driven by the moving component, the vertical slide is slidably installed on the horizontal slide, the vertical slide is arranged vertically with the horizontal slide, a plurality of electromagnetic coils are installed on the horizontal slide and the vertical slide, the clamping component is slidably installed on the vertical slide, magnets are installed on the vertical slide and the clamping component, the two magnets respectively match the plurality of electromagnetic coils on the horizontal slide and the vertical slide, the frame is installed on the horizontal slide, the fixed end of the push cylinder 2 is installed on the frame, and the lower end of the piston rod of the push cylinder 2 is installed with a suction cup The suction cup is located above the clamping component; after the bacterial species in the small culture dish is cultured at the first level, the moving component moves the crossbeam and moves the cross slide on the crossbeam, so that the clamping component clamps the small culture dish and moves the small culture dish to the top of the large culture dish, and the multiple electromagnetic coils on the cross slide change polarity according to the program, so that the magnets on the longitudinal slide are attracted by the multiple electromagnetic coils, so that the longitudinal slide moves and positions along the cross slide, and at the same time, the multiple electromagnetic coils on the longitudinal slide change polarity according to the program, so that the magnets on the clamping component are attracted by the multiple electromagnetic coils, so that the clamping component moves and positions along the longitudinal slide, so that the clamping component drives the small culture dish to move gradually under the suction cup, and in this process, the piston rod of the push cylinder 2 extends downward, so that the suction cup absorbs the corresponding small pieces of culture medium in the small culture dish, and after the small culture dish moves out from under the suction cup, the suction cup releases the small pieces of culture medium, and cooperates with the action of the moving component to evenly distribute the small pieces of culture medium in the large culture dish, thereby achieving even seed distribution and good practicality.

[0012] Preferably, the clamping assembly includes a double-headed push cylinder and two clamping plates. The double-headed push cylinder is slidably mounted on the longitudinal slide table through a slider. One of the magnets is mounted on the double-headed push cylinder, and the other magnet is mounted on a side wall of the longitudinal slide table. The two clamping plates are relatively mounted on the two piston rod ends of the double-headed push cylinder. When the two piston rods of the double-headed push cylinder are relatively extended, the two clamping plates open, and when the two piston rods of the double-headed push cylinder are relatively contracted, the two clamping plates are close to clamp the two sides of the small culture plate.

[0013] Preferably, the moving assembly includes a screw, a servo motor, two longitudinal slides, multiple drive wheels and two synchronous belts. The screw is rotatably installed on the cross beam, and the screw is connected to the cross slide by a screw nut. The servo motor is installed at one end of the cross beam, and the output shaft of the servo motor is connected to the screw. The two longitudinal slides are respectively installed on the upper part of the chamber of the incubator. Multiple drive wheels are installed on the two longitudinal slides. Two synchronous belts are respectively mounted on the multiple drive wheels on the two longitudinal slides, and the two ends of the cross beam are respectively connected to the two synchronous belts. The servo motor drives the screw to rotate, and the screw drives the cross slide along the longitudinal direction of the beam through the screw nut. The multiple drive wheels rotate synchronously to drive the two synchronous belts to rotate synchronously, so that the two synchronous belts drive the cross beam to move along the two longitudinal slides, so as to evenly distribute the small pieces of culture medium on the large culture plate, which has good practicality.

[0014] Preferably, it also includes a mounting base and a driving motor, the mounting base is mounted on the middle inner wall of the chamber of the incubator, one side of the middle support plate is rotatably connected to the mounting base through a rotating shaft, the driving motor is mounted on the mounting base, and the output shaft of the driving motor is transmission-connected to the rotating shaft of the middle support plate; when the large culture tray is lifted by the lifting mechanism, the driving motor drives the middle support plate to rotate downward, so that the middle support plate is close to the inner wall of the chamber of the incubator to avoid interfering with the lifting of the large culture tray; when the large culture tray reaches the middle of the chamber of the incubator, the driving motor drives the middle support plate to rotate to a horizontal position, so that multiple lamp beads are aligned with the bottom of the large culture tray, which has good practicality.

[0015] Preferably, the lifting mechanism comprises a column, two driving wheels 2, a synchronous belt 2, two moving blocks and a fixed block, the column is vertically mounted at the rear of the lower middle part of the chamber of the incubator, the two driving wheels 2 are rotatably mounted at the bottom and top of the column respectively, the synchronous belt 2 is sleeved on the two driving wheels 2, the two moving blocks are respectively mounted on both sides of the synchronous belt 2, the fixed blocks are mounted on the side walls of the plurality of large culture trays, and both sides of the fixed blocks are provided with card slots matching the moving blocks; when the large culture tray is placed into the lower part of the chamber of the incubator through the lower opening of the incubator, a card slot on one side of the fixed block of the large culture tray is engaged with the corresponding moving block, the two driving wheels 2 rotate to drive the synchronous belt 2 to rotate, the synchronous belt 2 drives the moving block to rise, and at the same time drives the other moving block to descend, thereby lifting and raising the large culture tray and supporting it by the middle support plate, when the large culture tray is taken out through the middle opening, the two driving wheels 2 reverse and drive the synchronous belt 2 to reverse, so that the next large culture tray can be raised, which has good practicality.

[0016] A method for culturing Trichoderma aspergillus species of the present invention uses the Trichoderma aspergillus species culturing device, comprising the following steps:

[0017] S1. Turn on the heating system to preheat the chamber of the incubator;

[0018] S2, prepare the culture medium, add the culture medium to the small culture dish and the large culture dish respectively, inoculate the small culture dish with Trichoderma acanthosporum, place the small culture dish on the upper support plate through the upper opening of the incubator, place the large culture dish on the vibration plate through the lower opening, and close the door;

[0019] S3, the bacteria in the small culture dish are cultured in the first stage, and at the same time, the vibration motor is turned on to drive the vibration plate to vibrate the large culture dish, so that the culture medium in the large culture dish is evenly mixed;

[0020] S4. After the culture medium in the large culture dish is mixed evenly, the lifting mechanism lifts the large culture dish to the middle of the chamber of the incubator, and the middle support plate rotates to a horizontal position to support the large culture dish;

[0021] S5. After the primary culture of the bacteria in the small culture dish is completed, the dividing component divides the culture medium in the small culture dish into small pieces, and the seed separation mechanism evenly distributes the small pieces of culture medium in the small culture dish into the large culture dish for secondary culture;

[0022] S6. During the secondary culture of the bacteria in the large culture dish, multiple lamp beads and multiple light sensing elements are used to intervene in the growth of the bacteria, so that the bacteria in the large culture dish develop evenly;

[0023] S7. After the secondary culture of the bacteria in the large culture dish is completed, the door of the chamber is opened and the large culture dish is taken out from the opening;

[0024] In order to improve the utilization rate, when the secondary bacterial strain culture is carried out on the large culture plate, multiple small culture plates can be placed on the upper support plate to carry out the primary bacterial strain culture at the same time, and another large culture plate can be placed on the vibration plate to prepare the culture medium.

[0025] Preferably, the specific process of multiple lamp beads and multiple light sensing elements intervening in the growth of bacteria is:

[0026] A plurality of lamp beads illuminate the bottom of the large culture dish, so that the light emitted by the plurality of lamp beads passes through the large culture dish, culture medium and bacterial colony to illuminate a plurality of photosensitive elements. The crossbeam moves above the large culture dish, and the plurality of photosensitive elements sense light to generate electrical signals of different intensities. The light transmittance of the large culture dish is detected and the positions where the light transmittance is too strong and too weak are located through the electrical signals and the positions where the electrical signals are generated. The bacterial strains are poorly developed at the positions where the light transmittance is too strong, and the illumination of the plurality of lamp beads at the positions where the light transmittance is too weak is strengthened, and the illumination of the plurality of lamp beads at the positions where the light transmittance is too weak is reduced, so that the temperature of the culture medium at the positions where the light transmittance is too strong in the large culture dish is appropriately increased, and the temperature of the culture medium at the positions where the light transmittance is too weak in the large culture dish is appropriately reduced, thereby controlling the temperature of the local part of the culture dish, and allowing the plurality of lamp beads to automatically and accurately intervene according to the development of the bacterial strains in the large culture dish.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] 1. A small amount of bacterial strains are used for primary culture in a small culture dish. When the culture medium in the small culture dish is full of bacterial strains, the seed separation mechanism evenly distributes the small pieces of bacterial strains obtained after the primary culture in the small culture dish to the large culture dish, so that the bacterial strains are cultured and developed in the culture medium of the large culture dish for secondary culture, and the use cost is reduced;

[0029] 2. During the bacterial culture process in the large culture dish, multiple lamp beads and multiple light sensing elements cooperate to automatically and accurately intervene according to the development of the bacterial species in the large culture dish, so that the bacterial colonies in the large culture dish develop and distribute evenly in the later stage of culture, thereby improving the efficiency of bacterial culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a partial cross-sectional structural schematic diagram of the present invention;

[0031] Figure 2 It is a schematic diagram of the side section structure of the present invention;

[0032] Figure 3 It is a schematic diagram of the structure of the present invention when multiple doors are opened;

[0033] Figure 4 It is a front cross-sectional structural schematic diagram of the present invention;

[0034] Figure 5 It is a schematic diagram of the structure of the present invention when multiple doors are in a closed state;

[0035] Figure 6 It is a structural schematic diagram of the upper support plate, the small culture tray and the splitting component;

[0036] Figure 7 It is a structural diagram of the seed separation mechanism;

[0037] Figure 8 It is a structural diagram of the middle support plate, lamp beads, mounting base and drive motor;

[0038] Fig. 9 It is a structural schematic diagram of the seed separation mechanism, beam and light sensing element;

[0039] Fig.10 It is a schematic diagram of the structure of the large culture tray and the lifting mechanism;

[0040] Fig.11 It is a schematic diagram of the structure of a large culture dish, a vibration plate, a spring and other structures.

[0041] Markings in the attached figure: 1. incubator; 2. heating system; 3. box door; 4. upper support plate; 5. small culture tray; 6. large culture tray; 7. middle support plate; 8. lamp beads; 9. crossbeam; 10. light sensing element; 11. push cylinder one; 12. blade assembly; 13. horizontal slide; 14. vertical slide; 15. electromagnetic coil; 16. magnet; 17. frame; 18. push cylinder two; 19. suction cup; 20. double-head push cylinder; 21. clamp; 22. lead screw; 23. servo motor; 24. vertical slide; 25. drive wheel one; 26. synchronous belt one; 27. mounting seat; 28. drive motor; 29. ​​column; 30. drive wheel two; 31. synchronous belt two; 32. moving card block; 33. fixed card block; 34. vibration plate; 35. spring; 36. vibration motor. DETAILED DESCRIPTION

[0042] In order to facilitate understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive. Example 1

[0043] like Figures 1 to 5 , Figure 8 , Fig.10 and Fig.11As shown, a Trichoderma spinulosa culture device includes a culture box 1 and a heating system 2. A chamber is arranged inside the culture box 1, and the heating system 2 is installed in the chamber of the culture box 1. The heating system 2 includes a heater and a plurality of temperature sensors; it also includes three doors 3, an upper support plate 4, a plurality of small culture trays 5, a plurality of large culture trays 6, a middle support plate 7, a plurality of lamp beads 8, a crossbeam 9 and a plurality of light sensing elements 10. The front end surface of the culture box 1 is provided with an upper opening, a middle opening and a lower opening from top to bottom. The three doors 3 are hinged on the upper opening, the middle opening and the lower opening respectively. The upper support plate 4 is installed in the chamber of the culture box 1, and the upper support plate 4 is flush with the upper opening. The upper support plate 4 holds A plurality of small culture trays 5 are provided, and a dividing assembly is installed above the upper support plate 4, and the dividing assembly is used to evenly divide the culture medium in the small culture trays 5. The bottom of the large culture tray 6 is transparent, and a dividing mechanism is installed on the upper part of the chamber of the incubator 1. A lifting mechanism is installed in the middle and lower part of the chamber of the incubator 1, and the lifting mechanism is used to lift the plurality of large culture trays 6 from the lower part of the chamber of the incubator 1 to the middle of the chamber, and the dividing mechanism is used to evenly distribute the small pieces of culture medium in the small culture trays 5 to the large culture trays 6 located in the middle of the chamber of the incubator 1. The middle support plate 7 is rotatably installed on the middle inner wall of the incubator 1, and a plurality of lamp beads 8 are arranged in a matrix on the middle support plate 7, and the plurality of lamp beads 8 are oriented toward the chamber of the incubator 1. The bottom of the large culture tray 6 at the bottom, the seeding mechanism includes a crossbeam 9, the crossbeam 9 moves above the large culture tray 6, and a plurality of light sensing elements 10 are arranged in a matrix at the bottom of the crossbeam 9; it also includes a mounting seat 27 and a drive motor 28, the mounting seat 27 is mounted on the inner wall of the middle part of the chamber of the incubator 1, one side of the middle support plate 7 is rotatably connected with the mounting seat 27 through a rotating shaft, the drive motor 28 is mounted on the mounting seat 27, and the output shaft of the drive motor 28 is transmission-connected with the rotating shaft of the middle support plate 7; the lifting mechanism includes a column 29, two driving wheels 30, a synchronous belt 31, two moving blocks 32 and a fixed block 33, and the column 29 is vertically mounted on the lower middle part of the chamber of the incubator 1 At the rear, two driving wheels 30 are rotatably mounted on the bottom and top of the column 29, a synchronous belt 31 is mounted on the two driving wheels 30, two moving blocks 32 are respectively mounted on both sides of the synchronous belt 31, and fixed blocks 33 are mounted on the side walls of multiple large culture trays 6, and both sides of the fixed blocks 33 are provided with slots matching the two moving blocks 32; it also includes a vibration plate 34, multiple springs 35 and a vibration motor 36, the vibration plate 34 is elastically mounted on the lower part of the chamber of the incubator 1 through multiple springs 35, the large culture tray 6 is placed on the vibration plate 34, the vibration plate 34 is flush with the lower opening of the incubator 1, and a vibration motor 36 is installed at the bottom of the vibration plate 34.

[0044] When working, the heating system 2 heats the air in the chamber of the incubator 1, and detects the temperature values ​​at various locations through multiple temperature sensors, and adjusts the heating system 2 according to the temperature values, so as to accurately control the temperature in the chamber of the incubator 1. When the temperature reaches the required temperature, the upper opening of the box door 3 is opened, and the small culture tray 5 containing the inoculated gel culture medium is placed on the upper support plate 4, so that a small amount of bacteria are cultured in the small culture tray 5. The lower opening of the box door 3 is opened, and various raw materials of the culture medium are added to the large culture tray 6. The large culture tray 6 is placed on the vibration plate 34 through the lower opening of the incubator 1, and the vibration motor 36 is turned on to vibrate the vibration plate 34. Through the elastic support of multiple springs 35, the vibration plate 34 vibrates the large culture tray 6. , so that the raw materials in the large culture tray 6 are fully mixed to form a culture medium, the large culture tray 6 is preheated, the two driving wheels 2 30 rotate to drive the synchronous belt 2 31 to rotate, the synchronous belt 2 31 drives the moving card block 32 to rise, and the one side slot of the fixed card block 33 of the large culture tray 6 is engaged with the corresponding moving card block 32, and at the same time drives the other moving card block 32 to descend, thereby lifting the large culture tray 6. When the large culture tray 6 is lifted by the lifting mechanism, the driving motor 28 drives the middle support plate 7 to rotate downward, so that the middle support plate 7 is close to the inner wall of the chamber of the incubator 1 to avoid interfering with the lifting of the large culture tray 6. When the large culture tray 6 reaches the middle of the chamber of the incubator 1, the driving motor 28 drives the middle support plate 7 to rotate to a horizontal level, and the middle support plate 7 supports the large culture tray 6, so that the multiple lamp beads 8 are aligned with the large culture tray 6. The bottom of the plate 6, when the culture medium in the small culture plate 5 is full of bacterial strains, the dividing component evenly divides the culture medium in the small culture plate 5 into multiple small pieces, the seed dividing mechanism moves the small culture plate 5 to the top of the large culture plate 6, and the seed dividing mechanism evenly distributes the culture medium and the small pieces of bacterial strains in the small culture plate 5 to the large culture plate 6, so that the bacterial strains undergo secondary culture development in the culture medium of the large culture plate 6, and multiple interventions are performed during the bacterial strain cultivation in the large culture plate 6, and the interventions are: multiple lamp beads 8 are turned on to illuminate the bottom of the large culture plate 6, so that the multiple lamp beads 8 emit light that penetrates the large culture plate 6, the culture medium and the bacterial colony to illuminate the multiple light sensing elements 10, the crossbeam 9 moves above the large culture plate 6, and the multiple light sensing elements 10 sense the light transmittance of the large culture plate 6 Detect and locate the positions where the light transmission is too strong and too weak. The bacterial strains are poorly developed at the positions where the light transmission is too strong. The controller of the multiple lamp beads 8 strengthens the illumination of the multiple lamp beads 8 at the positions where the light transmission is too strong, and reduces the illumination of the multiple lamp beads 8 at the positions where the light transmission is too weak, so that the temperature of the culture medium at the positions where the light transmission is too strong in the large culture dish 6 is appropriately increased, and the temperature of the culture medium at the positions where the light transmission is too weak in the large culture dish 6 is appropriately reduced, thereby controlling the temperature of the local part of the culture dish, so that the multiple lamp beads 8 automatically and accurately intervene according to the development of the bacterial strains in the large culture dish 6. In order to improve the heating intervention effect of the lamp beads 8, the lamp beads 8 can use full-spectrum lamp beads or corresponding spectrum lamp beads, so that the bacterial colonies in the large culture dish 6 develop and are evenly distributed in the later stage of cultivation, thereby improving the efficiency of bacterial cultivation.When the secondary culture of the bacteria in the large culture tray 6 is completed, the large culture tray 6 is taken out through the middle opening. Example 2

[0045] like Figures 6 to 9 As shown, on the basis of Example 1, the segmentation component includes a push cylinder 11 and a blade group 12, the fixed end of the push cylinder 11 is installed on the top wall of the incubator 1, the blade group 12 is installed at the lower end of the piston rod of the push cylinder 11, the blade group 12 is located above the upper support plate 4, the blade group 12 is composed of a plurality of transverse blades and a plurality of longitudinal blades staggered to form a plurality of squares, and the blade group 12 matches the small culture tray 5; the seed separation mechanism includes a moving component, a clamping component and a pick-and-place component, the moving component is installed on the upper part of the chamber of the incubator 1, the moving component drives the crossbeam 9 to move horizontally, the pick-and-place component is installed on the crossbeam 9, the pick-and-place component moves longitudinally along the crossbeam 9, and the clamping component is installed On the pick-and-place assembly, the clamping assembly is used to clamp the small culture plate 5, and the pick-and-place assembly is used to take out the small piece of culture medium in the small culture plate 5 and put it into the large culture plate 6. The pick-and-place assembly includes a horizontal slide 13, a vertical slide 14, an electromagnetic coil 15, a magnet 16, a frame 17, a push cylinder 18 and a suction cup 19. The horizontal slide 13 is longitudinally slidably installed on the crossbeam 9, the horizontal slide 13 is driven by a moving assembly, the vertical slide 14 is slidably installed on the horizontal slide 13, and the vertical slide 14 is arranged vertically with the horizontal slide 13. A plurality of electromagnetic coils 15 are installed on the horizontal slide 13 and the vertical slide 14, the clamping assembly is slidably installed on the vertical slide 14, and the vertical slide 14 and the clamping assembly are A magnet 16 is installed, and two magnets 16 are matched with a plurality of electromagnetic coils 15 on the horizontal slide 13 and the vertical slide 14 respectively. A frame 17 is installed on the horizontal slide 13, and a fixed end of a push cylinder 18 is installed on the frame 17. A suction cup 19 is installed at the lower end of the piston rod of the push cylinder 18, and the suction cup 19 is located above the clamping assembly; the clamping assembly includes a double-headed push cylinder 20 and two clamping plates 21. The double-headed push cylinder 20 is slidably installed on the vertical slide 14 through a slider, and a magnet 16 is installed on the double-headed push cylinder 20, and another magnet 16 is installed on a side wall of the vertical slide 14, and two clamping plates 21 are installed opposite to each other at the ends of the two piston rods of the double-headed push cylinder 20; The dynamic component includes a crossbeam 9, a screw rod 22, a servo motor 23, two longitudinal slides 24, a plurality of driving wheels 25 and two synchronous belts 26. The screw rod 22 is rotatably installed on the crossbeam 9. The screw rod 22 is connected to the cross slide 13 through a screw nut. The servo motor 23 is installed on the crossbeam 9. The output shaft of the servo motor 23 is connected to the screw rod 22. The two longitudinal slides 24 are respectively installed on the upper part of the chamber of the incubator 1. Multiple driving wheels 25 are installed on the two longitudinal slides 24. Two synchronous belts 26 are respectively mounted on the multiple driving wheels 25 on the two longitudinal slides 24. The two ends of the crossbeam 9 are respectively connected to the two synchronous belts 26.

[0046] The small culture tray 5 is filled with gel or solid culture medium, and the culture medium is inoculated with bacteria. The small culture tray 5 is placed on the upper support plate 4, so that the bacteria in the small culture tray 5 undergo primary culture. When the bacteria in the small culture tray 5 are fully developed and produce a large number of spore bacteria, the piston rod of the push cylinder 11 extends downward to allow the blade group 12 to extend into the small culture tray 5 to evenly divide the culture medium into multiple small pieces. To prevent the small pieces of culture medium from being stuck in the blade group 12, a lubricant can be pre-coated on the blades of the blade group 12, or a non-stick coating can be provided on the surface of the blade group 12. After the bacteria in the small culture tray 5 undergo primary culture, the two piston rods of the double-head push cylinder 20 are relatively extended, and the two splints 21 are opened, and the servo motor 23 drives the screw rod 22 to rotate, and the screw rod 22 drives the horizontal slide 13 along the longitudinal direction of the beam 9 through the screw nut, and the multiple driving wheels 25 rotate synchronously to drive the two synchronous belts 26 to rotate synchronously, so that the two synchronous belts 26 drive the beam 9 to move along the two longitudinal slides 24, so that the two splints 21 are respectively positioned At both sides of the small culture tray 5, when the two piston rods of the double-headed push cylinder 20 are relatively contracted, the two clamps 21 approach to clamp the two sides of the small culture tray 5, and the multiple electromagnetic coils 15 on the horizontal slide 13 change polarity according to the program, so that the magnet 16 on the vertical slide 14 is attracted by the multiple electromagnetic coils 15, so that the vertical slide 14 moves and positions along the horizontal slide 13. At the same time, the multiple electromagnetic coils 15 on the vertical slide 14 change polarity according to the program, so that the magnet 16 on the clamping assembly is attracted by the multiple electromagnetic coils 15, so that the clamping assembly moves and positions along the vertical slide 14, so that the clamping assembly drives the small culture tray 5 to move gradually under the suction cup 19. In this process, the piston rod of the push cylinder 18 extends downward, so that the suction cup 19 absorbs the corresponding small pieces of culture medium in the small culture tray 5. After the small culture tray 5 moves out from under the suction cup 19, the suction cup 19 releases the small pieces of culture medium, and the servo motor 23 and the multiple drive wheels 25 run to evenly distribute the small pieces of culture medium on the large culture tray 6, thereby realizing automatic and uniform inoculation.

[0047] The method for culturing Trichoderma acanthosporum using the culture device disclosed in Example 1 is as follows:

[0048] S1, turning on the heating system 2 to preheat the chamber of the incubator 1;

[0049] S2, prepare the culture medium, add the culture medium to the small culture dish 5 and the large culture dish 6 respectively, inoculate the small culture dish 5 with the Trichoderma species, place the small culture dish 5 on the upper support plate 4 through the upper opening of the incubator 1, place the large culture dish 6 on the vibration plate 34 through the lower opening, and close the door 3;

[0050] S3, the bacteria in the small culture dish 5 are cultured in the first level, and at the same time, the vibration motor 36 is turned on to drive the vibration plate 34 to vibrate the large culture dish 6, so that the culture medium in the large culture dish 6 is evenly mixed;

[0051] S4, after the culture medium in the large culture tray 6 is evenly mixed, the lifting mechanism lifts the large culture tray 6 to the middle of the chamber of the incubator 1, and the middle support plate 7 rotates to a horizontal position to support the large culture tray 6;

[0052] S5, after the primary culture of the bacteria in the small culture dish 5 is completed, the dividing component divides the culture medium in the small culture dish 5 into small pieces, and the seed separation mechanism evenly distributes the small pieces of culture medium in the small culture dish 5 and inoculates them into the large culture dish 6 for secondary culture;

[0053] S6, during the secondary culture of the bacteria in the large culture tray 6, the growth of the bacteria is intervened by the cooperation of multiple lamp beads 8 and multiple light sensing elements 10. The specific process of intervention is as follows:

[0054] A plurality of lamp beads 8 illuminate the bottom of the large culture dish 6, so that the light emitted by the plurality of lamp beads 8 passes through the large culture dish 6, the culture medium and the bacterial colony to illuminate the plurality of light sensing elements 10, the crossbeam 9 moves above the large culture dish 6, and the plurality of light sensing elements 10 sense light to generate electrical signals of different intensities, and the light transmittance of the large culture dish 6 is detected and the positions where the light transmittance is too strong and too weak are located through the electrical signals and the positions where the electrical signals are generated, the bacterial strains at the positions where the light transmittance is too strong are poorly developed, and the controller of the plurality of lamp beads 8 strengthens the illumination of the plurality of lamp beads 8 at the positions where the light transmittance is too strong, and reduces the illumination of the plurality of lamp beads 8 at the positions where the light transmittance is too weak, so that the temperature of the culture medium at the positions where the light transmittance is too strong in the large culture dish 6 is appropriately increased, and the temperature of the culture medium at the positions where the light transmittance is too weak in the large culture dish 6 is appropriately reduced, thereby controlling the temperature of the local part of the culture dish, so that the plurality of lamp beads 8 automatically and accurately intervene according to the development of the bacterial strains in the large culture dish 6, so that the bacterial strains in the large culture dish 6 develop evenly;

[0055] S7, after the secondary culture of the bacteria in the large culture tray 6 is completed, the door 3 is opened and the large culture tray 6 is taken out from the opening;

[0056] In order to improve the utilization rate, when the large culture tray 6 is used for secondary bacterial culture, multiple small culture trays 5 can be placed on the upper support plate 4 to simultaneously carry out primary bacterial culture, and another large culture tray 6 can be placed on the vibration plate 34 to prepare the culture medium.

[0057] like Figures 1 to 11As shown, a kind of Trichoderma aspergillus strain culture device and culture method of the present invention, when working, firstly, start the heating system 2 to preheat the chamber of the incubator 1, prepare the culture medium, add the culture medium into the small culture plate 5 and the large culture plate 6 respectively, inoculate the Trichoderma aspergillus strain into the small culture plate 5, put the small culture plate 5 on the upper support plate 4 through the upper opening of the incubator 1, put the large culture plate 6 on the vibration plate 34 through the lower opening, close the box door 3, the strain in the small culture plate 5 is cultured in the first stage, and at the same time, the vibration motor 36 is turned on to drive the vibration plate 34 to vibrate the large culture plate 6, so that the large culture plate 6 is inoculated with the Trichoderma aspergillus strain. The culture medium in the culture tray 6 is mixed evenly, and then the two driving wheels 2 30 rotate to drive the synchronous belt 2 31 to rotate, and the synchronous belt 2 31 drives the moving card block 32 to rise, and the one side slot of the fixed card block 33 of the large culture tray 6 is engaged with the corresponding moving card block 32, and at the same time drives the other moving card block 32 to descend, so that the large culture tray 6 is lifted to the middle of the chamber of the incubator 1, and the middle support plate 7 rotates to a horizontal position to support the large culture tray 6. Then, after the first-level culture of the bacteria in the small culture tray 5 is completed, the piston rod of the push cylinder 11 extends downward to make the blade group 12 extend into the small culture tray 5 to evenly divide the culture medium into multiple The seed separation mechanism evenly distributes the small pieces of culture medium in the small culture tray 5 into the large culture tray 6 for secondary culture. During the culture of the bacteria in the large culture tray 6, multiple lamp beads 8 are turned on to illuminate the bottom of the large culture tray 6, so that the multiple lamp beads 8 emit light through the large culture tray 6, the culture medium and the bacterial colony to illuminate the multiple light sensing elements 10. The crossbeam 9 moves above the large culture tray 6, and the multiple light sensing elements 10 sense the light to detect the light transmittance of the large culture tray 6 and locate the positions where the light transmittance is too strong and too weak. The bacteria are poorly developed at the position where the light transmittance is too strong, and the controllers of the multiple lamp beads 8 strengthen The illumination of multiple lamp beads 8 at the position where the light transmission is too strong is increased, and the illumination of multiple lamp beads 8 at the position where the light transmission is too weak is reduced, so that the temperature of the culture medium at the position where the light transmission is too strong in the large culture tray 6 is appropriately increased, and the temperature of the culture medium at the position where the light transmission is too weak in the large culture tray 6 is appropriately reduced, thereby controlling the temperature of the local part of the culture tray, and allowing the multiple lamp beads 8 to automatically and accurately intervene according to the development of the bacterial species in the large culture tray 6, so that the bacterial species in the large culture tray 6 develop evenly. After the secondary culture of the bacterial species in the large culture tray 6 is completed, finally open the box door 3 and take out the large culture tray 6 from the opening.

[0058] The main functions achieved by the present invention are:

[0059] 1. It can automatically and accurately intervene according to the development of bacterial strains, so that the bacterial strains develop evenly and improve the efficiency of bacterial strain cultivation;

[0060] 2. Two-stage culture is adopted to reduce the input of original strains, and two-stage culture can be carried out simultaneously, with high work efficiency and low cost;

[0061] 3. It can pre-treat the culture medium and has good practicality;

[0062] 4. The primary cultured bacteria can be equally divided and inoculated into the large culture tray 6, with a high degree of automation.

[0063] The installation mode, connection mode or setting mode of the Trichoderma aspergillus culture device and the culture method of the present invention are all common mechanical modes, and any mode that can achieve its beneficial effects can be implemented; the culture box 1, heating system 2, box door 3, small culture tray 5, large culture tray 6, lamp beads 8, light sensing element 10, push cylinder 11, blade group 12, electromagnetic coil 15, magnet 16, push cylinder 2 18, suction cup 19, double-head push cylinder 20, screw rod 22, servo motor 23, drive wheel 1 25, synchronous belt 1 26, drive motor 28, drive wheel 2 30, synchronous belt 2 31, spring 35, vibration motor 36 of the Trichoderma aspergillus culture device and the culture method of the present invention are purchased on the market, and technicians in the industry only need to install and operate them according to the accompanying instruction manual, without the need for technicians in this field to make creative labor.

[0064] The above are only preferred embodiments of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A Trichoderma spinulosa culture device, comprising an incubator (1) and a heating system (2), wherein a chamber is arranged inside the incubator (1), and the heating system (2) is installed in the chamber of the incubator (1), and the heating system (2) comprises a heater and a plurality of temperature sensors; characterized in that: The incubator (1) further comprises three doors (3), an upper support plate (4), a plurality of small culture trays (5), a plurality of large culture trays (6), a middle support plate (7), a plurality of lamp beads (8), a crossbeam (9), a plurality of light sensing elements (10), a vibration plate (34), a plurality of springs (35) and a vibration motor (36). The front end surface of the incubator (1) is provided with an upper opening, a middle opening and a lower opening from top to bottom. The three doors (3) are hinged on the upper opening, the middle opening and the lower opening respectively. The upper support plate (4) is installed in the chamber of the incubator (1). 4) is flush with the upper opening, the upper support plate (4) holds a plurality of small culture plates (5), a dividing assembly is installed above the upper support plate (4), and the dividing assembly is used to evenly divide the culture medium in the small culture plates (5), a vibration plate (34) is elastically installed at the lower part of the chamber of the incubator (1) through a plurality of springs (35), the vibration plate (34) is flush with the lower opening of the incubator (1), a vibration motor (36) is installed at the bottom of the vibration plate (34), and the vibration plate (34) holds a plurality of large culture plates (6), and the bottom of the large culture plates (6) is transparent; A seeding mechanism is installed at the upper part of the chamber of the incubator (1), and a lifting mechanism is installed at the middle and lower part of the chamber of the incubator (1). The lifting mechanism is used to lift a plurality of large culture trays (6) from the lower part of the chamber of the incubator (1) to the middle part of the chamber. The seeding mechanism is used to evenly distribute small pieces of culture medium in the small culture trays (5) to the large culture trays (6) located in the middle part of the chamber of the incubator (1). The middle support plate (7) is rotatably installed on the middle inner wall of the incubator (1). A plurality of lamp beads (8) are arranged in a matrix on the middle support plate (7). The plurality of lamp beads (8) face the bottom of the large culture tray (6) located in the middle part of the chamber of the incubator (1). The seeding mechanism comprises a crossbeam (9). The crossbeam (9) moves above the large culture tray (6). A plurality of light sensing elements (10) are arranged in a matrix at the bottom of the crossbeam (9).

2. A Trichoderma aspergillus species culture device as claimed in claim 1, characterized in that: The segmentation assembly comprises a push cylinder (11) and a blade group (12); the fixed end of the push cylinder (11) is mounted on the top wall of the incubator (1); the blade group (12) is mounted on the lower end of the piston rod of the push cylinder (11); the blade group (12) is located above the upper support plate (4); the blade group (12) is composed of a plurality of horizontal blades and a plurality of vertical blades interlaced to form a plurality of squares; the blade group (12) matches the small culture tray (5).

3. A Trichoderma aspergillus species culture device as claimed in claim 1, characterized in that: The sorting mechanism comprises a moving component, a clamping component and a pick-and-place component. The moving component is mounted on the upper part of the chamber of the incubator (1). The moving component drives the crossbeam (9) to move horizontally. The pick-and-place component is mounted on the crossbeam (9). The pick-and-place component moves longitudinally along the crossbeam (9). The clamping component is mounted on the pick-and-place component. The clamping component is used to clamp the small culture tray (5). The pick-and-place component is used to take out a small piece of culture medium from the small culture tray (5) and put it into the large culture tray (6). The pick-and-place component comprises a horizontal slide (13), a vertical slide (14), an electromagnetic coil (15), a magnet (16), a frame (17), a push cylinder (18) and a suction cup (19). The horizontal slide (13) is slidably mounted on the crossbeam (9). The horizontal slide (13) is composed of The moving component is driven, the longitudinal slide (14) is slidably mounted on the transverse slide (13), the longitudinal slide (14) is arranged vertically with the transverse slide (13), a plurality of electromagnetic coils (15) are mounted on both the transverse slide (13) and the longitudinal slide (14), the clamping component is slidably mounted on the longitudinal slide (14), magnets (16) are mounted on both the longitudinal slide (14) and the clamping component, the two magnets (16) are matched with the plurality of electromagnetic coils (15) on the transverse slide (13) and the longitudinal slide (14), the frame (17) is mounted on the transverse slide (13), the fixed end of the push cylinder (18) is mounted on the frame (17), the lower end of the piston rod of the push cylinder (18) is mounted with a suction cup (19), and the suction cup (19) is located above the clamping component.

4. A Trichoderma aspergillus species culture device as claimed in claim 3, characterized in that: The clamping assembly comprises a double-headed push cylinder (20) and two clamping plates (21), wherein the double-headed push cylinder (20) is slidably mounted on a longitudinal slide table (14) via a slider, wherein one magnet (16) is mounted on the double-headed push cylinder (20), and the other magnet (16) is mounted on a side wall of the longitudinal slide table (14), and the two clamping plates (21) are mounted oppositely on the two piston rod ends of the double-headed push cylinder (20).

5. A Trichoderma aspergillus species culture device as claimed in claim 3, characterized in that: The moving assembly comprises a screw (22), a servo motor (23), two longitudinal slides (24), a plurality of driving wheels (25) and two synchronous belts (26); the screw (22) is rotatably mounted on the crossbeam (9); the screw (22) is connected to the cross slide (13) by a screw nut; the servo motor (23) is mounted on one end of the crossbeam (9); the output shaft of the servo motor (23) is connected to the screw (22); the two longitudinal slides (24) are respectively mounted on the upper part of the chamber of the incubator (1); the two longitudinal slides (24) are each equipped with a plurality of driving wheels (25); the two synchronous belts (26) are respectively mounted on the plurality of driving wheels (25) on the two longitudinal slides (24); and the two ends of the crossbeam (9) are respectively connected to the two synchronous belts (26).

6. A Trichoderma aspergillus species culture device as claimed in claim 1, characterized in that: It also includes a mounting seat (27) and a drive motor (28), wherein the mounting seat (27) is mounted on the inner wall of the middle part of the chamber of the incubator (1), one side of the middle support plate (7) is rotatably connected to the mounting seat (27) via a rotating shaft, the drive motor (28) is mounted on the mounting seat (27), and the output shaft of the drive motor (28) is drivingly connected to the rotating shaft of the middle support plate (7).

7. A Trichoderma aspergillus species culture device as claimed in claim 1, characterized in that: The lifting mechanism comprises a column (29), two driving wheels (30), a synchronous belt (31), two moving blocks (32) and a fixed block (33); the column (29) is vertically mounted at the rear of the lower middle portion of the chamber of the incubator (1); the two driving wheels (30) are rotatably mounted at the bottom and top of the column (29), the synchronous belt (31) is sleeved on the two driving wheels (30), the two moving blocks (32) are respectively mounted on both sides of the synchronous belt (31), and the fixed blocks (33) are mounted on the side walls of the plurality of large culture trays (6); and both sides of the fixed blocks (33) are provided with slots matching the moving blocks (32).

8. A method for cultivating Trichoderma acanthosporum, characterized in that: Using the Trichoderma spinulosa species culture device according to claim 1 comprises the following steps: S1, turning on the heating system (2) to preheat the chamber of the incubator (1); S2, preparing a culture medium, and respectively adding the culture medium to the small culture plate (5) and the large culture plate (6), inoculating the small culture plate (5) with the Trichoderma species, placing the small culture plate (5) on the upper support plate (4) through the upper opening of the incubator (1), placing the large culture plate (6) on the vibration plate (34) through the lower opening, and closing the incubator door (3); S3, the bacteria in the small culture plate (5) are cultured in the first stage, and at the same time, the vibration motor (36) is turned on to drive the vibration plate (34) to vibrate the large culture plate (6), so that the culture medium in the large culture plate (6) is evenly mixed; S4. After the culture medium in the large culture tray (6) is evenly mixed, the lifting mechanism lifts the large culture tray (6) to the middle of the chamber of the incubator (1), and the middle support plate (7) rotates to a horizontal position to support the large culture tray (6); S5, after the primary culture of the bacterial strain in the small culture plate (5) is completed, the dividing component divides the culture medium in the small culture plate (5) into small pieces, and the seed separation mechanism evenly distributes the small pieces of culture medium in the small culture plate (5) and inoculates them into the large culture plate (6) for secondary culture; S6. During the secondary culture of the bacteria in the large culture dish (6), the growth of the bacteria is intervened by the cooperation of the plurality of lamp beads (8) and the plurality of light sensing elements (10), so that the bacteria in the large culture dish (6) develop evenly. The specific process of the plurality of lamp beads (8) and the plurality of light sensing elements (10) intervening in the growth of the bacteria is as follows: A plurality of lamp beads (8) illuminate the bottom of the large culture dish (6), so that the light emitted by the plurality of lamp beads (8) passes through the large culture dish (6), the culture medium and the bacterial colony and illuminates the plurality of light sensing elements (10). The crossbeam (9) moves above the large culture dish (6), and the plurality of light sensing elements (10) sense light and generate electrical signals of different intensities. The light transmittance of the large culture dish (6) is detected and the positions where the light transmittance is too strong and too weak are located through the electrical signals and the positions where the electrical signals are generated. The bacterial strains at the positions where the light transmittance is too strong are poorly developed. The illumination of the plurality of lamp beads (8) at the positions where the light transmittance is too strong is increased, and the illumination of the plurality of lamp beads (8) at the positions where the light transmittance is too weak is reduced, so that the temperature of the culture medium at the positions where the light transmittance is too strong in the large culture dish (6) is appropriately increased, and the temperature of the culture medium at the positions where the light transmittance is too weak in the large culture dish (6) is appropriately reduced, thereby controlling the temperature of the local part of the culture dish, so that the plurality of lamp beads (8) automatically and accurately intervene according to the development of the bacterial strains in the large culture dish (6); S7. After the secondary culture of the bacteria in the large culture tray (6) is completed, the box door (3) is opened and the large culture tray (6) is taken out from the opening.

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