A tumor cell cluster sorting and filtering device for brain tumor experiments

Abstract

The invention discloses a tumor cell cluster sorting and filtering device for brain tumor experiments, relates to the field of tumor cell cluster sorting, and solves the problem that the sieve holes of the existing tumor cell cluster sorting and filtering device for brain tumor experiments are easily clogged when in use and it is difficult to sort and collect the clogged objects during the cleaning process, resulting in low sorting efficiency. The invention comprises a body, a filtering mechanism, a dredging mechanism and a conveying mechanism, wherein the filtering mechanism comprises a screening cylinder, the dredging mechanism comprises an air blowing pipe and a cam, and the conveying mechanism comprises a conveying pipe, a collecting frame and a collecting plate. The tumor cell cluster sorting and filtering device for brain tumor experiments is convenient for sorting and filtering the cell clusters by driving the screening cylinder to rotate, and at the same time, the air blowing pipe is linked to blow air downward to the top of the screening cylinder, driving the cam to vibrate the upper outer wall of the screening cylinder, and dredging the sieve holes, collecting the screened cell clusters through the collecting frame, and collecting the large-sized cells that are shaken off by the dredging mechanism through the collecting plate.

Description

Technical Field

[0001] The invention relates to the technical field of tumor cell cluster sorting, and in particular to a tumor cell cluster sorting and filtering device for brain tumor experiments. Background Art

[0002] Brain tumors are also called intracranial tumors. Their etiology is still unknown, so it is necessary to conduct research and experiments on them. Tumors that occur in the brain, meninges, pituitary gland, cranial nerves, cerebral blood vessels, and residual embryonic tissues are called primary intracranial tumors; those that metastasize from malignant tumors in other organs and tissues of the body to the brain are called secondary intracranial tumors. In the course of their work, doctors in the Department of Oncology often need to sort and filter tumor cell clusters through a sorting and filtering device, that is, fresh tissue dispersion is added to the sorting and filtering device to sort and filter the tumor cell clusters. Since the size of larger tissues such as adipose tissue, membrane tissue, and connective tissue in the tissue dispersion is hundreds of microns, and the size of tumor cell clusters in the tissue dispersion is generally tens of microns, larger tissues often block the filter plate in the sorting and filtering device, affecting the sorting progress of tumor cell clusters.

[0003] Existing sorting and filtering devices mostly use flat screens for screening, and clean the cell clusters inside the screen holes by shaking the screen, but due to the influence of gravity, the cleaned cell clusters will still move into the screen holes, causing blockage again, affecting the subsequent sorting efficiency. To this end, we propose a tumor cell cluster sorting and filtering device for brain tumor experiments. Summary of the invention

[0004] The purpose of the present invention is to provide a tumor cell cluster sorting and filtering device for brain tumor experiments, which is convenient for improving sorting efficiency, reducing sieve hole blockage and capable of discharging and collecting blockages, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a tumor cell cluster sorting and filtering device for brain tumor experiments, comprising a body, a filtering mechanism, a dredging mechanism and a conveying mechanism, wherein the two sides of the body are respectively fixedly connected with a first side plate and a second side plate, the filtering mechanism comprises a screening cylinder installed in the body, which is used to drive the screening cylinder to rotate, thereby sorting and filtering the internal cell clusters, the dredging mechanism comprises an air blowing pipe fixedly installed between the first side plate and the second side plate, the air blowing pipe is located above the screening cylinder and opens downward, a rotating rod is rotatably connected between the first side plate and the second side plate, and a plurality of cams are evenly and fixedly connected to the rotating rod, which are used to link the air blowing pipe downward when the screening cylinder rotates. Gas is blown out from the top of the screening cylinder, and at the same time, the rotating rod is driven to rotate, so that the cam vibrates the upper outer wall of the screening cylinder to clear the sieve holes. The conveying mechanism includes a conveying pipe installed inside the screening cylinder, and a collecting frame is slidably connected to the bottom of the body in a horizontal direction. A collecting plate is provided on the upper side of the conveying pipe, and an output port connected to the interior of the screening cylinder is opened on the lower side of the conveying pipe, which is used to input cell clusters to be screened into the screening cylinder through the output port on the conveying pipe, collect the screened tumor cell clusters through the collecting frame, and collect the large-sized cells shaken off by the clearing mechanism through the collecting plate, so as to improve the sorting efficiency, reduce the blockage of the sieve holes, and discharge and collect the blockages.

[0006] Preferably, the filtering mechanism also includes a rotating disk rotatably connected to the second side plate, a plurality of groups of connecting rods are evenly and fixedly connected to the side of the rotating disk, a threaded ring is rotatably connected to the first side plate, and the ends of the plurality of connecting rods away from the rotating disk are all fixedly connected to the threaded ring, the outer wall of the screening cylinder is evenly provided with a plurality of groups of limiting grooves that can be slidably plugged with the inner walls of the connecting rods in a horizontal direction, the outer wall of the conveying pipe is rotatably connected to a threaded disk threadedly connected to the inner wall of the threaded ring, and the body is provided with a driving member for driving the rotating disk to rotate, so as to facilitate the sorting and filtering of tumor cell clusters.

[0007] Preferably, the conveying mechanism also includes a moving platform installed on the side of the body, one end of the conveying pipe is connected to a feed hopper, a socket is fixedly connected to the feed hopper, a plug-in slot is provided on the moving platform and is plugged into the socket in a vertical direction, the side of the collecting frame close to the first side panel is fixedly connected to the side of the moving platform, the collecting frame passes through the first side panel and is slidably connected to the first side panel in a horizontal direction, one end of the conveying pipe can be rotatably plugged into the rotating disk, so as to facilitate the input of the cell clusters to be screened into the screening cylinder through the output port on the conveying pipe, the screened tumor cell clusters are collected by the collecting frame, and the large-sized cells shaken off by the dredging mechanism are collected by the collecting disk.

[0008] Preferably, the dredging mechanism also includes a connecting pipe rotatably connected to the second side plate, the connecting pipe passes through the second side plate, one end of the connecting pipe is connected to the blowing pipe, the outer wall of the connecting pipe is fixedly connected with an outer tooth ring, one end of the rotating rod is fixedly connected with a driving gear meshing with the outer tooth ring, the driving member is used to synchronously drive the connecting pipe to rotate, and a blowing member is provided on the connecting pipe for blowing air into the blowing pipe during rotation, so as to facilitate cleaning and dredging of cells blocked in the sieve holes of the screening cylinder.

[0009] Preferably, the driving member includes a driving motor fixedly mounted on the machine body, the output end of the driving motor is coaxially fixedly connected with a driving shaft, one end of the driving shaft is provided with a reducer, the output end of the reducer is coaxially fixedly connected with the rotating disk, a first pulley is coaxially fixedly connected to the driving shaft, a second pulley is coaxially fixedly connected to the connecting pipe, the outer wall of the first pulley is transmission-connected with a transmission belt transmission-connected to the second pulley, so as to synchronously drive the rotating disk and the connecting pipe to rotate.

[0010] Preferably, the blowing member includes an air inlet pipe fixedly mounted on the machine body, one end of the connecting pipe is fixedly connected to a wind wheel, the outer wall of the wind wheel is rotatably connected to the inner wall of the air inlet pipe, so as to facilitate blowing into the blowing pipe during the rotation of the connecting pipe.

[0011] Preferably, a plug-in block is fixedly connected to the bottom of the collection tray, and a docking groove capable of being plugged into the plug-in block along a vertical direction is provided on the upper side of the delivery pipe, so as to facilitate the disassembly and assembly of the collection tray.

[0012] Preferably, a filter is fixedly connected to the air inlet pipe to facilitate filtering and removing impurities from the gas to avoid affecting internal cell components.

[0013] Preferably, a door is hinged on the upper side of the machine body to facilitate operations such as cleaning and maintenance of the interior of the machine body.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present invention provides a tumor cell cluster sorting and filtering device for brain tumor experiments, which solves the problem that the sieve holes of the existing tumor cell cluster sorting and filtering devices for brain tumor experiments are easily clogged when in use and it is difficult to sort and collect the clogged materials during the cleaning process, resulting in low sorting efficiency. It is convenient to input the cell clusters to be screened into the screening cylinder through the output port on the conveying pipe, and drive the screening cylinder to rotate, so as to sort and filter the internal cell clusters. When the screening cylinder rotates, the air blowing pipe is linked to blow gas downward to the top of the screening cylinder, and at the same time drive the rotating rod to rotate, so that the cam vibrates the upper outer wall of the screening cylinder to dredge the sieve holes, collect the screened tumor cell clusters through the collecting frame, and collect the large-sized cells that are shaken off by the dredging mechanism through the collecting plate, so that the sorting and dredging efficiency is high.

[0016] 2. The device has a compact structure and comprehensive functions. Each structure is easy to disassemble and maintain. The cam strikes the connecting rod, which effectively avoids direct impact on the surface of the screening drum and causes damage to the screening drum, thereby extending the service life of the screening drum. At the same time, the overall shaking of the connecting rod can make the shaking frequency of each position on the upper side of the screening drum more uniform. During the dredging process, the blockages can be automatically sorted and collected to avoid falling back into the dispersion liquid for continued mixing and affecting the screening efficiency, thereby reducing the probability of subsequent blockages. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 It is a schematic diagram of the internal structure of the machine body of the present invention;

[0019] Figure 3 It is a schematic diagram of the local structure of the driving member of the present invention;

[0020] Figure 4 for Figure 3 A magnified image of the middle A area;

[0021] Figure 5 This is a schematic diagram of the structure of the mobile station of the present invention in the pulled-out state;

[0022] Figure 6 It is an exploded view of the local structure of the conveying mechanism of the present invention;

[0023] Figure 7 for Figure 6 Enlarged view of area B;

[0024] Figure 8 It is a partial structural cross-sectional view of the dredging mechanism of the present invention;

[0025] Fig. 9 for Figure 8 Enlarged view of area C in the middle;

[0026] Fig.10 It is a partial structural cross-sectional view of the filtering mechanism of the present invention;

[0027] Fig.11 This is a schematic diagram of the structure of the screening drum of the present invention in a disassembled state;

[0028] Fig.12 for Fig.11 Enlarged view of area D in the middle.

[0029] In the figure: 1-body; 2-first side plate; 3-second side plate; 4-filter mechanism; 5-dredging mechanism; 6-air blowing pipe; 7-rotating rod; 8-cam; 9-conveying mechanism; 10-conveying pipe; 11-collecting frame; 12-collecting plate; 13-output port; 14-rotating plate; 15-connecting rod; 16-threaded ring; 17-limiting groove; 18-threaded plate; 19-driving member; 20-moving platform; 21- Feed hopper; 22-socket; 23-socket slot; 24-connecting pipe; 25-external gear ring; 26-driving gear; 27-blowing piece; 28-driving motor; 29-driving shaft; 30-reducing machine; 31-first pulley; 32-second pulley; 33-transmission belt; 34-inlet pipe; 35-wind wheel; 36-socket block; 37-docking slot; 38-filter screen; 39-box door; 40-screening cylinder. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Example 1: Please refer to Figure 1-Figure 12The figure shows a tumor cell cluster sorting and filtering device for brain tumor experiments, comprising a body 1, a filtering mechanism 4, a dredging mechanism 5 and a conveying mechanism 9. A box door 39 is hinged on the upper side of the body 1. The first side plate 2 and the second side plate 3 are fixedly connected on both sides of the body 1. The filtering mechanism 4 comprises a screening cylinder 40 installed in the body 1, which is used to drive the screening cylinder 40 to rotate, so as to sort and filter the internal cell clusters. The dredging mechanism 5 comprises an air blowing pipe 6 fixedly installed between the first side plate 2 and the second side plate 3. The air blowing pipe 6 is located above the screening cylinder 40 and opens downward. A rotating rod 7 is rotatably connected between the first side plate 2 and the second side plate 3. A plurality of cams 8 are evenly and fixedly connected to the rotating rod 7, which are used to link the air blowing pipe 6 to blow gas downward to the top of the screening cylinder 40 when the screening cylinder 40 rotates, and at the same time drive the rotating rod 7 to rotate, so that the cam 8 vibrates the upper outer wall of the screening cylinder 40 to dredge the sieve holes.

[0032] The conveying mechanism 9 includes a conveying pipe 10 installed inside the screening cylinder 40, a collecting frame 11 is slidably connected to the bottom of the body 1 in the horizontal direction, a collecting tray 12 is provided on the upper side of the conveying pipe 10, a plug-in block 36 is fixedly connected to the bottom of the collecting tray 12, a docking groove 37 that can be plugged with the plug-in block 36 in the vertical direction is provided on the upper side of the conveying pipe 10, and an output port 13 connected to the inside of the screening cylinder 40 is provided on the lower side of the conveying pipe 10, which is used to input the cell clusters to be screened into the screening cylinder 40 through the output port 13 on the conveying pipe 10, collect the screened tumor cell clusters through the collecting frame 11, and collect the large-sized cells shaken off by the dredging mechanism 5 through the collecting tray 12.

[0033] The filtering mechanism 4 also includes a rotating disk 14 rotatably connected to the second side plate 3, and multiple groups of connecting rods 15 are evenly and fixedly connected to the side of the rotating disk 14. A threaded ring 16 is rotatably connected to the first side plate 2, and the ends of the multiple groups of connecting rods 15 away from the rotating disk 14 are fixedly connected to the threaded ring 16. The outer wall of the screening drum 40 is evenly provided with multiple groups of limiting grooves 17 that can be slidably plugged with the inner wall of the connecting rod 15 in a horizontal direction. The outer wall of the conveying pipe 10 is rotatably connected to a threaded disk 18 threadedly connected to the inner wall of the threaded ring 16. A driving member 19 for driving the rotating disk 14 to rotate is provided on the body 1.

[0034] The conveying mechanism 9 also includes a moving platform 20 installed on the side of the body 1. One end of the conveying pipe 10 is connected to the feed hopper 21, and a socket 22 is fixedly connected to the feed hopper 21. The moving platform 20 is provided with a plug-in groove 23 which is plugged into the socket 22 in the vertical direction. The side of the collecting frame 11 close to the first side plate 2 is fixedly connected to the side of the moving platform 20. The collecting frame 11 passes through the first side plate 2 and is slidably connected to the first side plate 2 in the horizontal direction. One end of the conveying pipe 10 can be rotatably plugged into the rotating disk 14.

[0035] In the present embodiment, before use, the threaded disk 18 is rotated to release the threaded connection with the threaded ring 16, and the movable platform 20 is slid outward, driving the hopper 21, the conveying pipe 10 and the collecting frame 11 to move outward from the machine body 1, and one end of the limiting groove 17 on the cleaned screening drum 40 is aligned with the connecting rod 15, and the screening drum 40 is pushed into the threaded ring 16, and then the movable platform 20 is pushed toward the machine body 1, so that the collecting frame 11 is inserted into the interior of the machine body 1, and the conveying pipe 10 is inserted into the screening drum 40. The threaded disk 18 is rotated to threadably connect with the threaded ring 16 to complete the installation of the screening drum 40, and vice versa, the screening drum 40 can be disassembled.

[0036] By injecting the tissue dispersion to be sorted into the feed hopper 21, the liquid flows into the screening cylinder 40 through the output port 13 at the bottom of the conveying tube 10, and accumulates at the bottom of the inner wall of the screening cylinder 40. At this time, the driving member 19 drives the rotating disk 14 to rotate slowly, so that the connecting rod 15 drives the screening cylinder 40 to rotate together. After the liquid is screened by the sieve holes in the screening cylinder 40, it falls into the collecting frame 11 at the bottom for collection, and a tumor cell cluster with a smaller diameter is obtained, while the large-diameter cells that cannot pass through the sieve holes remain in the screening cylinder 40.

[0037] It is worth noting that when a blockage occurs in the sieve hole, the sieve hole will rotate to the top as the sieve drum 40 rotates. At this time, the blockage is in the lower area of ​​the sieve hole. Blowing air from top to bottom through the air blowing pipe 6 can assist in blowing the blockage out of the sieve hole and drop it into the collecting plate 12 for collection. At the same time, the unblocking mechanism 5 will also drive the rotating rod 7 to rotate, so that the cam 8 can knock on the outer wall of the connecting rod 15 rotated to the upper position, and the upper end of the sieve drum 40 is driven to vibrate through the vibration of the connecting rod 15, thereby improving the efficiency of the falling of the blockage in the upper sieve hole. The knocking of the connecting rod 15 by the cam 8 can effectively avoid the direct impact on the surface of the sieve drum 40 to cause damage to the sieve drum 40, thereby extending the service life of the sieve drum 40. At the same time, the shaking frequency of each position on the upper side of the sieve drum 40 can be made more uniform through the overall shaking of the connecting rod 15.

[0038] After the dredging is completed, the screening cylinder 40 will continue to rotate slowly to the bottom for subsequent sorting and screening operations, which effectively ensures that during the sorting process, there are always relatively unblocked sieve holes moving to the bottom for sorting operations, and the sorting efficiency is higher. At the same time, the blocked large-diameter cells can also be collected through the collecting plate 12 to prevent them from falling back into the dispersion after dredging and affecting the subsequent sorting efficiency. After the sorting is completed, the threaded plate 18 is turned out, and the movable platform 20 can be pulled to move the collecting frame 11 and the collecting bucket out of the body 1, so as to facilitate the collection of the required cells. The collecting plate 12 is pulled out upwards, so that the plug-in block 36 and the docking groove 37 are unplugged, and the collecting plate 12 can be removed. The hopper 21 is pulled upwards to release the plug-in state of the plug-in seat 22 and the plug-in groove 23, and the hopper 21 and the conveying pipe 10 can be removed as a whole, so as to facilitate the subsequent extraction of the screening cylinder 40 from the body 1 for cleaning and replacement.

[0039] Example 2: Please refer to Figure 1-Figure 12 Embodiment 2 is described. This embodiment further describes Embodiment 1. The dredging mechanism 5 shown in the figure also includes a connecting pipe 24 rotatably connected to the second side plate 3. The connecting pipe 24 passes through the second side plate 3. One end of the connecting pipe 24 is connected to the blowing pipe 6. An outer tooth ring 25 is fixedly connected to the outer wall of the connecting pipe 24. A driving gear 26 meshing with the outer tooth ring 25 is fixedly connected to one end of the rotating rod 7. The driving member 19 is used to synchronously drive the connecting pipe 24 to rotate. A blowing member 27 is provided on the connecting pipe 24 for blowing air into the blowing pipe 6 during rotation.

[0040] The driving member 19 includes a driving motor 28 fixedly mounted on the body 1, and the model of the driving motor 28 is preferably YYHS-40. The output end of the driving motor 28 is coaxially fixedly connected with a driving shaft 29, and a reducer 30 is provided at one end of the driving shaft 29. The output end of the reducer 30 is coaxially fixedly connected to the rotating disk 14, and a first pulley 31 is coaxially fixedly connected to the driving shaft 29. A second pulley 32 is coaxially fixedly connected to the connecting tube 24, and the outer wall of the first pulley 31 is transmission-connected to a transmission belt 33 transmission-connected to the second pulley 32.

[0041] In this embodiment, the driving motor 28 is started to drive the driving shaft 29 to rotate. After being decelerated by the reducer 30, the rotating disk 14 can be driven to rotate slowly, thereby realizing the rotation and sorting operation of the screening drum 40. At the same time, the driving shaft 29 drives the first pulley 31 so that the transmission belt 33 drives the second pulley 32 to rotate, and the second pulley 32 drives the connecting tube 24 to rotate, so that the outer gear ring 25 drives the surrounding meshing driving gear 26 to rotate (the rotating rod 7 can be one or two symmetrically distributed on both sides of the blowing pipe 6), thereby driving the rotating rod 7 and the cam 8 to rotate. The cam 8 can impact the connecting rod 15 rotated to the upper position to cause the upper side of the screening drum 40 to vibrate, and the connecting tube 24 is blown by the blowing member 27. The gas is input into the blowing pipe 6 through the connecting tube 24 and blown out through the bottom, so that the gas can be blown down from the top of the screening drum 40 from top to bottom, and the cells blocked in the sieve holes are blown out and fall into the collecting plate 12 for collection.

[0042] Example 3: Please refer to Figure 1-Figure 12 Embodiment 3 is described. This embodiment further describes Embodiment 1. The blowing member 27 shown in the figure includes an air inlet pipe 34 fixedly mounted on the machine body 1. One end of the connecting pipe 24 is fixedly connected to a wind wheel 35. The outer wall of the wind wheel 35 is rotatably connected to the inner wall of the air inlet pipe 34. A filter screen 38 is fixedly connected inside the air inlet pipe 34.

[0043] In this embodiment, when the connecting pipe 24 rotates, the wind wheel 35 is driven to rotate, and the gas is sucked in from the outside through the air inlet pipe 34 and blown to the air blowing pipe 6. The gas can be filtered and removed by the filter 38 to avoid affecting the internal cell components. At the same time, a one-way exhaust grille is provided on the box door 39 to discharge the internal gas to the outside in a one-way manner. Opening the top box door 39 can perform operations such as flushing and maintenance on the inside of the body 1.

[0044] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0045] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A tumor cell cluster sorting and filtering device for brain tumor experiments, characterized in that: include: A machine body (1), wherein two sides of the machine body (1) are respectively fixedly connected with a first side plate (2) and a second side plate (3); Also includes: A filtering mechanism (4), the filtering mechanism (4) comprising a sieving cylinder (40) installed in the body (1), and used for driving the sieving cylinder (40) to rotate, thereby sorting and filtering the cell clusters inside; a dredging mechanism (5), the dredging mechanism (5) comprising an air blowing pipe (6) fixedly mounted between the first side plate (2) and the second side plate (3), the air blowing pipe (6) being located above the screening drum (40) and opening downward, a plurality of groups of rotating rods (7) being rotatably connected between the first side plate (2) and the second side plate (3), a plurality of groups of cams (8) being evenly and fixedly connected to the rotating rods (7) for driving the air blowing pipe (6) to blow gas downward toward the top of the screening drum (40) when the screening drum (40) rotates, and driving the rotating rods (7) to rotate, so that the cams (8) vibrate the upper outer wall of the screening drum (40) to dredge the screening holes; A conveying mechanism (9), the conveying mechanism (9) comprising a conveying pipe (10) installed inside the screening cylinder (40), a collecting frame (11) being slidably connected to the bottom of the body (1) in a horizontal direction, a collecting plate (12) being provided on the upper side of the conveying pipe (10), an output port (13) being provided on the lower side of the conveying pipe (10) and being connected to the inside of the screening cylinder (40), for inputting cell clusters to be screened into the screening cylinder (40) through the output port (13) on the conveying pipe (10), collecting the screened tumor cell clusters through the collecting frame (11), and collecting the large-sized cells shaken off by the dredging mechanism (5) through the collecting plate (12), a plug-in block (36) being fixedly connected to the bottom of the collecting plate (12), and a docking groove (37) being provided on the upper side of the conveying pipe (10) and being able to be plugged into the plug-in block (36) in a vertical direction; The filtering mechanism (4) further comprises a rotating disk (14) rotatably connected to the second side plate (3); a plurality of connecting rods (15) are evenly and fixedly connected to the side surface of the rotating disk (14); a threaded ring (16) is rotatably connected to the first side plate (2); the ends of the plurality of connecting rods (15) away from the rotating disk (14) are all fixedly connected to the threaded ring (16); the outer wall of the screening cylinder (40) is evenly provided with a plurality of limiting grooves (17) capable of slidingly plugging with the inner wall of the connecting rod (15) in a horizontal direction; the outer wall of the conveying pipe (10) is rotatably connected to a threaded disk (18) threadedly connected to the inner wall of the threaded ring (16); and the machine body (1) is provided with a threaded disk (18) for driving the rotating disk (14) to move forward. The conveying mechanism (9) further comprises a movable platform (20) mounted on the side of the machine body (1); one end of the conveying pipe (10) is connected to a feed hopper (21); a plug socket (22) is fixedly connected to the feed hopper (21); a plug slot (23) is provided on the movable platform (20) and is plugged into the plug socket (22) in a vertical direction; a side of the collecting frame (11) close to the first side plate (2) is fixedly connected to the side of the movable platform (20); the collecting frame (11) passes through the first side plate (2) and is slidably connected to the first side plate (2) in a horizontal direction; one end of the conveying pipe (10) can be rotatably plugged into the rotating disk (14).

2. The tumor cell cluster separation and filtration device for brain tumor experiments according to claim 1, characterized in that: The dredging mechanism (5) further comprises a connecting pipe (24) rotatably connected to the second side plate (3), the connecting pipe (24) passing through the second side plate (3), one end of the connecting pipe (24) being connected to the air blowing pipe (6), an outer toothed ring (25) being fixedly connected to the outer wall of the connecting pipe (24), one end of the rotating rod (7) being fixedly connected to a driving gear (26) meshing with the outer toothed ring (25), the driving member (19) being used to synchronously drive the connecting pipe (24) to rotate, and a blowing member (27) being provided on the connecting pipe (24) for blowing air into the air blowing pipe (6) during the rotation process.

3. The tumor cell cluster separation and filtration device for brain tumor experiments according to claim 2, characterized in that: The driving member (19) comprises a driving motor (28) fixedly mounted on the machine body (1); the output end of the driving motor (28) is coaxially fixedly connected to a driving shaft (29); a reducer (30) is provided at one end of the driving shaft (29); the output end of the reducer (30) is coaxially fixedly connected to the rotating disk (14); a first belt pulley (31) is coaxially fixedly connected to the driving shaft (29); a second belt pulley (32) is coaxially fixedly connected to the connecting pipe (24); and a transmission belt (33) is transmission-connected to the outer wall of the first belt pulley (31) and is transmission-connected to the second belt pulley (32).

4. The tumor cell cluster separation and filtration device for brain tumor experiments according to claim 2, characterized in that: The blowing member (27) comprises an air inlet pipe (34) fixedly mounted on the machine body (1); one end of the connecting pipe (24) is fixedly connected to a wind wheel (35); and an outer wall of the wind wheel (35) is rotatably connected to an inner wall of the air inlet pipe (34).

5. The tumor cell cluster separation and filtration device for brain tumor experiments according to claim 4, characterized in that: A filter screen (38) is fixedly connected inside the air intake pipe (34).

6. The tumor cell cluster separation and filtration device for brain tumor experiments according to claim 1, characterized in that: A door (39) is hingedly connected to the upper side of the machine body (1).

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