Liquid storage and release mechanism and liquid storage and release method for microfluidic chip

Abstract

The present invention relates to the field of microfluidic technology, and in particular to a liquid storage and release mechanism and a liquid storage and release method for a microfluidic chip. An embodiment of the present invention provides a liquid storage and release mechanism for a microfluidic chip, comprising a main body and a puncture device; the main body comprises adjacent sealed cavities and limiting bins, the sealed cavity comprises a first opening and a second opening whose opening directions are perpendicular to each other, the second opening is connected to the limiting bin, the first opening and the second opening are sealed with a sealing film, the puncture device comprises a first puncture member and a second puncture member, the first puncture member faces the first opening, the second puncture member penetrates into the limiting bin, and a limiting inclined surface is arranged on the inner wall of the limiting bin relative to the second opening. An embodiment of the present invention provides a liquid storage and release mechanism and a liquid storage and release method for a microfluidic chip, which can provide a liquid storage and release mechanism with a simple structure.

Description

Technical Field

[0001] The invention relates to the field of microfluidic technology, and in particular to a liquid storage and release mechanism for a microfluidic chip. Background Art

[0002] Nucleic acid detection is a technology for detecting biological genetic material, which is widely used in the fields of pathogenic microorganism detection and infectious disease diagnosis. In order to quickly and conveniently detect nucleic acids, the present invention uses microfluidic technology to develop a nucleic acid detection kit to detect samples to be tested, wherein the flow control of each liquid reagent stored in the kit is achieved by microfluidic technology. Specifically, microfluidic technology can control the release, flow, and distribution of fluids in the chip, and with the cooperation of external instruments, ultimately achieve nucleic acid detection.

[0003] In the related art, the functions of sealing and releasing the pre-stored reagents in the test kit are usually realized by using a sealing ring and a driving assembly. However, the sealing ring and the driving assembly have many parts, which increases the complexity of the microfluidic chip, and makes the overall microfluidic chip have many parts and a large volume, which is not convenient for production and transportation. Summary of the invention

[0004] The embodiments of the present invention provide a liquid storage and release mechanism and a liquid storage and release method for a microfluidic chip, which can provide a liquid storage and release mechanism with a simple structure.

[0005] In a first aspect, an embodiment of the present invention provides a liquid storage and release mechanism for a microfluidic chip, comprising a main body and a puncture device;

[0006] The main body comprises an adjacent sealing cavity and a limiting bin, the sealing cavity comprises a first opening and a second opening whose opening directions are perpendicular to each other, the second opening is in communication with the limiting bin, the first opening and the second opening are sealed with a sealing film, the puncture device comprises a first puncture member and a second puncture member, the first puncture member faces the first opening, the second puncture member penetrates into the limiting bin, and a limiting inclined surface is arranged on the inner wall of the limiting bin relative to the second opening;

[0007] When the fluid in the sealing cavity needs to be released, the puncture device is driven to move toward the main body so that the first puncture member punctures the sealing membrane at the first opening, and the second puncture member in the limiting bin is bent toward the second opening under the restriction of the limiting slope to puncture the sealing membrane at the second opening.

[0008] In a possible design, the second puncture member includes a guide portion and a puncture portion connected in sequence, a guide groove is provided in the limiting bin, the guide portion passes through the guide groove, and the guide groove is used to limit the second puncture member from moving in a preset direction.

[0009] In a possible design, the puncture portion is bent toward the sealing cavity.

[0010] In a possible design, a plate-shaped force-bearing plate is provided at a portion of the guide portion close to the puncture portion, and the force-bearing plate is used to cooperate with the limiting slope to control the deformation direction of the puncture portion to puncture the sealing membrane on the second opening.

[0011] In a possible design, the puncture device further includes a buckle, a locking position is provided in the limiting bin, and the buckle cooperates with the locking position to limit the position of the puncture device.

[0012] In a possible design, the locking position includes a first locking position and a second locking position. When the buckle is located at the first locking position, the puncture device does not puncture the sealing film on the first opening and the sealing film on the second opening. When the buckle is located at the second locking position, the puncture device punctures the sealing film on the first opening and the sealing film on the second opening.

[0013] In a possible design, the puncture device also includes a force-driven member, which includes a mounting plate and a driving surface obliquely arranged on the mounting plate, the first puncture member and the second puncture member are respectively installed on the side of the mounting plate away from the driving surface, and a driving device is arranged on one side of the driving surface, the driving device includes a plunger pump and a driving member, and the driving member is arranged on a reciprocating rod of the plunger pump, the reciprocating rod is parallel to the mounting plate, and moves along its axial direction through the reciprocating rod, and the driving member, the driving surface, the guide portion and the guide groove on the reciprocating rod cooperate to drive the puncture device to move along the preset direction; wherein, the plunger pump is connected to the microfluidic chip to provide it with power for fluid control.

[0014] In one possible design, a buffer chamber is connected to the bottom of the limiting bin. After the puncture device pierces the first opening and the second opening, the fluid in the sealed cavity flows into the buffer chamber through the limiting bin. The buffer chamber is connected to the fluid control unit of the microfluidic chip, and the fluid control unit is used to absorb the fluid in the buffer chamber according to instructions.

[0015] In a possible design, a plurality of sealed cavities are included, each of the sealed cavities corresponds to one of the limiting bins, one of the first piercing members, and one of the second piercing members, and the depths of the plurality of sealed cavities are different.

[0016] In a possible design, a guide bin 10 is further provided on the main body, and a support body is provided on the mounting plate. The support body is inserted into the guide bin 10 and cooperates with the guide bin 10 to control the mounting plate to move in a straight line.

[0017] In a possible design, the first piercing member is cross-shaped, and the mounting plate is provided with an air guide groove, one end of the air guide groove is located at the edge of the mounting plate, and the other end is located at the root of the first piercing member.

[0018] In a second aspect, an embodiment of the present invention further provides a liquid storage and release method for a microfluidic chip, based on any of the above-mentioned liquid storage and release devices, the liquid storage and release method comprises:

[0019] The puncture device is driven to move toward the main body so that the first puncture member punctures the sealing film at the first opening, and the second puncture member in the limiting bin is bent toward the second opening under the restriction of the limiting slope to puncture the sealing film at the second opening.

[0020] Compared with the prior art, the present invention has at least the following beneficial effects:

[0021] In this embodiment, the sealing cavity further comprises a first opening and a second opening, and a sealing film is arranged at the first opening and the second opening to seal and preserve the fluid in the sealing cavity. The limiting chamber and the sealing cavity are arranged back to back, and the limiting chamber and the sealing cavity are connected through the second opening. The first piercing member of the puncture device is arranged above the first opening, and the second piercing member is arranged inside the limiting chamber. When the fluid needs to be released, the puncture device can be driven to move, and the first piercing member and the second piercing member move downward, and the first piercing member directly pierces the sealing film at the first opening during the movement. At the same time, the second piercing member inside the limiting chamber moves toward the position where the second opening is located, and when the second piercing member approaches the second opening, it will contact the limiting inclined surface inside the limiting chamber. Continue to move the second piercing member, the limiting inclined surface forces the second piercing member to bend along its inclined surface, that is, bend toward the second opening, and then the second piercing member pierces the sealing film at the second opening. After the sealing film of the first opening and the sealing film of the second opening of the sealing cavity are all pierced, the fluid in the sealing cavity flows out to the limiting chamber through the second opening to realize the release of the fluid. The bottom of the limiting chamber can be directly or indirectly connected to the fluid control unit of the microfluidic chip to control the fluid released from the sealed cavity.

[0022] In this embodiment, the sealing and storage function can be achieved only by the cooperation of the sealing cavity with the first opening and the second opening and the sealing film. When the fluid needs to be released, it is only necessary to puncture the sealing film at the first opening and the second opening. The entire storage and release mechanism has a simple structure and convenient release operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 It is a partial structural schematic diagram of a liquid storage and release mechanism provided by an embodiment of the present invention;

[0025] Figure 2 is a schematic structural diagram of a piercing device provided by an embodiment of the present invention;

[0026] Figure 3 It is a schematic diagram of a fluid structure in a sealed state of a sealed cavity provided by an embodiment of the present invention;

[0027] Figure 4 It is a schematic diagram of a fluid structure in a sealed cavity punctured state provided by an embodiment of the present invention;

[0028] Figure 5 It is a schematic diagram of a flow chart of a liquid storage and release mechanism releasing fluid provided by an embodiment of the present invention;

[0029] Figure 6 is a structural schematic diagram of a liquid storage and release mechanism provided by an embodiment of the present invention;

[0030] Figure 7 It is a structural schematic diagram of a mounting plate and accessories of a liquid storage and release mechanism provided by an embodiment of the present invention;

[0031] Figure 8 is a structural schematic diagram of another liquid storage and release mechanism provided by an embodiment of the present invention;

[0032] Fig. 9 It is a side view of another liquid storage and release mechanism provided by an embodiment of the present invention.

[0033] In the figure:

[0034] 1- Sealed cavity;

[0035] 11- First opening;

[0036] 12- second opening;

[0037] 2-Limited bin;

[0038] 21- limiting inclined plane;

[0039] 22-guide groove;

[0040] 23-First card position;

[0041] 24-Second card position;

[0042] 3-first piercing member;

[0043] 4- second piercing member;

[0044] 41- guide part;

[0045] 411-stress plate;

[0046] 42-puncture part;

[0047] 5- Buckle;

[0048] 6-Mounting plate;

[0049] 61- air guide groove;

[0050] 62-support body;

[0051] 7- driving surface;

[0052] 8- plunger pump;

[0053] 81 - reciprocating rod;

[0054] 82-driving member;

[0055] 9- buffer chamber;

[0056] 10-Guide warehouse. DETAILED DESCRIPTION

[0057] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0058] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance; unless otherwise specified or explained, the term "multiple" refers to two or more; the terms "connected" and "fixed" should be understood in a broad sense, for example, "connected" can be a fixed connection, a detachable connection, an integral connection, or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0059] In the description of this specification, it should be understood that the directional words such as "upper" and "lower" described in the embodiments of the present invention are described at the angles shown in the drawings and should not be understood as limiting the embodiments of the present invention. In addition, in the context, it should also be understood that when it is mentioned that an element is connected to another element "upper" or "lower", it can not only be directly connected to another element "upper" or "lower", but also indirectly connected to another element "upper" or "lower" through an intermediate element.

[0060] like Figures 1 to 5 As shown, an embodiment of the present invention provides a liquid storage and release mechanism for a microfluidic chip, comprising a main body and a puncture device;

[0061] The main body includes an adjacent sealed cavity 1 and a limiting chamber 2, the sealed cavity 1 includes a first opening 11 and a second opening 12 whose opening directions are perpendicular to each other, the second opening 12 is connected to the limiting chamber 2, the first opening 11 and the second opening 12 are sealed with a sealing film, the puncture device includes a first puncture member 3 and a second puncture member 4, the first puncture member 3 faces the first opening 11, the second puncture member 4 penetrates into the limiting chamber 2, and a limiting inclined surface 21 is provided on the inner wall of the limiting chamber 2 relative to the second opening 12;

[0062] When the fluid in the sealing cavity 1 needs to be released, the puncture device is driven to move toward the main body so that the first puncture member 3 punctures the sealing membrane at the first opening 11, and the second puncture member 4 in the limiting chamber 2 is bent toward the second opening 12 under the restriction of the limiting slope 21 to puncture the sealing membrane at the second opening 12.

[0063] In this embodiment, the sealing chamber 1 further comprises a first opening 11 and a second opening 12, and a sealing film is disposed at each of the first opening 11 and the second opening 12 to seal and preserve the fluid in the sealing chamber 1. The limiting chamber 2 is disposed back to back with the sealing chamber 1, and the limiting chamber 2 and the sealing chamber 1 are connected through the second opening 12. The first piercing member of the puncture device is disposed above the first opening 11, and the second piercing member is disposed inside the limiting chamber 2. When the fluid needs to be released, the puncture device can be driven to move, and the first piercing member and the second piercing member move downward, and the first piercing member directly pierces the sealing film at the first opening 11 during the movement. At the same time, the second piercing member inside the limiting chamber 2 moves toward the position where the second opening 12 is located, and when the second piercing member approaches the second opening 12, it will contact the limiting inclined surface 21 inside the limiting chamber 2. The second piercing member continues to move, and the limiting inclined surface 21 forces the second piercing member to bend along its inclined surface, that is, bend toward the second opening 12, and then the second piercing member pierces the sealing film at the second opening 12. After the sealing films of the first opening 11 and the second opening 12 of the sealed cavity 1 are all punctured, the fluid in the sealed cavity 1 flows out through the second opening 12 into the limiting chamber 2 to release the fluid. The bottom of the limiting chamber 2 can be directly or indirectly connected to the fluid control unit of the microfluidic chip to control the fluid released from the sealed cavity 1.

[0064] In this embodiment, the sealing and storage function can be achieved only by the cooperation of the sealing cavity 1 with the first opening 11 and the second opening 12 and the sealing film. When the fluid needs to be released, it is only necessary to puncture the sealing film at the first opening 11 and the second opening 12. The entire storage and release mechanism has a simple structure and convenient release operation.

[0065] It should be noted that the fluid can flow out freely only after the first opening 11 and the second opening 12 of the sealed cavity 1 are punctured at the same time. If only the second opening 12 at the bottom is punctured, the fluid in the sealed cavity 1 will be hindered by the atmospheric pressure and will be difficult to flow out.

[0066] In some embodiments of the present invention, the second puncture member 4 includes a guide portion 41 and a puncture portion 42 connected in sequence, a guide groove 22 is provided in the limiting bin 2, the guide portion 41 passes through the guide groove 22, and the guide groove 22 is used to limit the second puncture member 4 to move along a preset direction.

[0067] In this embodiment, in order to enable the second piercing member 4 to move accurately to the second opening 12 of the sealing cavity 1, a guide groove 22 is provided in the limiting chamber 2, and the guide portion 41 of the second piercing member 4 moves along a preset direction under the action of the guide groove 22 so that the piercing portion 42 of the second piercing member 4 can accurately reach the second opening 12.

[0068] It should be noted that the second puncture member 4 is a part of the puncture device, that is, the cooperation between the guide groove 22 and the guide portion 41 can enable the entire puncture device to move along a preset direction.

[0069] In some embodiments of the present invention, the puncture portion 42 is bent toward the sealing cavity 1 .

[0070] In this embodiment, the puncture portion 42 is bent toward the sealing cavity 1 to facilitate puncturing the sealing membrane at the second opening 12 .

[0071] In some embodiments of the present invention, a plate-shaped force-bearing plate 411 is provided at a position of the guide portion 41 close to the puncture portion 42 . The force-bearing plate 411 is used to cooperate with the limiting slope 21 to control the deformation direction of the puncture portion 42 to puncture the sealing film on the second opening 12 .

[0072] In this embodiment, the force-bearing plate 411 has a larger contact area with the limiting inclined surface 21 than other thinner parts of the guide portion 41 , which is more conducive to controlling its bending direction, ie, bending toward the second opening 12 .

[0073] In some embodiments of the present invention, the puncture device further includes a buckle 5, and a locking position is provided in the limiting chamber 2, and the buckle 5 and the locking position cooperate to limit the position of the puncture device.

[0074] In this embodiment, the puncture device is further provided with a buckle 5, which can cooperate with the clamping position in the limiting bin 2 to fix the puncture device at different positions.

[0075] In some embodiments of the present invention, the locking position includes a first locking position 23 and a second locking position 24. When the buckle 5 is located on the first locking position 23, the puncture device does not puncture the sealing film on the first opening 11 and the sealing film on the second opening 12. When the buckle 5 is located on the second locking position 24, the puncture device punctures the sealing film on the first opening 11 and the sealing film on the second opening 12.

[0076] In this embodiment, when the buckle 5 is opened in the first position 23, the puncture device is located in the upper part of the sealing cavity 1 and the limiting chamber 2 as a whole, and the first opening 11 and the second opening 12 are not punctured. When it is necessary to puncture the sealing cavity 1, the puncture device is driven to move, and the buckle 5 is disengaged from the first position 23 and moves downward to the second position 24. During the movement, the first puncture member 3 and the second puncture member 4 puncture the sealing film at the first opening 11 and the sealing film at the second opening 12 respectively. After completing the puncture task, the buckle 5 is locked in the second position 24 so that the puncture device is still in a fixed state.

[0077] It is understandable that, in order to prevent the puncture device from being separated from the second clamping position 24, the second clamping position 24 can be set as an angular clamping position, and the buckle 5 is provided with a barb structure.

[0078] In some embodiments of the present invention, the puncture device also includes a force-driven member 82, which includes a mounting plate 6 and a driving surface 7 obliquely arranged on the mounting plate 6. The first puncture member 3 and the second puncture member 4 are respectively installed on the side of the mounting plate 6 away from the driving surface 7. A driving device is arranged on one side of the driving surface 7. The driving device includes a plunger pump 8 and a driving member 82. The driving member 82 is arranged on a reciprocating rod 81 of the plunger pump 8. The reciprocating rod 81 is parallel to the mounting plate 6 and moves along its axial direction through the reciprocating rod 81. The driving member 82, the driving surface 7, the guide portion 41 and the guide groove 22 on the reciprocating rod 81 cooperate to drive the puncture device to move in a preset direction; wherein, the plunger pump 8 is connected to the microfluidic chip to provide it with power for fluid control.

[0079] In this embodiment, the driving device can not only drive the puncture device to move to puncture the first opening 11 and the second opening 12 of the sealed cavity 1, but the plunger pump 8 of the driving device can also provide power for fluid control for the microfluidic chip.

[0080] In this embodiment, in order to achieve a precise pumping function, the reciprocating rod 81 may be a screw rod.

[0081] It should be noted that after the driving device drives the puncture device to perform the puncture operation, the puncture device is fixed in the puncture state due to the cooperation between the second clamping position 24 and the buckle 5. At this time, the reciprocating rod 81 of the plunger pump 8 can still reciprocate to provide power.

[0082] In some embodiments of the present invention, a buffer chamber 9 is connected to the bottom of the limiting chamber 2. After the puncture device pierces the first opening 11 and the second opening 12, the fluid in the sealed chamber 1 flows into the buffer chamber 9 through the limiting chamber 2. The buffer chamber 9 is connected to the fluid control unit of the microfluidic chip, and the fluid control unit is used to absorb the fluid in the buffer chamber 9 according to instructions.

[0083] In this embodiment, after the sealing cavity 1 is punctured, the fluid can flow into the buffer cavity 9 through the limiting bin 2 for standby use.

[0084] Please refer to Figure 6 In some embodiments of the present invention, a plurality of sealed cavities 1 are included, each of which corresponds to a limiting bin 2, a first piercing member 3 and a second piercing member 4, and the depths of the plurality of sealed cavities 1 are different.

[0085] In this embodiment, the sealed chambers 1 with different bottom heights correspond to the limiting bins 2 with different bottom heights and the second openings 12 at different positions, and the fluid in the sealed chamber 1 is released in sequence according to the downward pressure of the puncture device.

[0086] like Figure 7 and Figure 8As shown, in one embodiment of the present invention, a guide bin 10 is further provided on the main body, and a support body 62 is provided on the mounting plate 6. The support body is inserted into the guide bin 10 and cooperates with it to control the mounting plate 6 to move in a straight line.

[0087] In this embodiment, the second puncture member is relatively soft and may bend during the puncture process. Therefore, the guide bin 10 and the support body 62 are designed to cooperate with each other so that the puncture members on the mounting plate can complete the puncture along a straight line in a preset direction.

[0088] like Figure 7 and Fig. 9 As shown, in one embodiment of the present invention, the first piercing member is cross-shaped, the mounting plate is provided with an air guide groove 61, one end of the air guide groove 61 is located at the edge of the mounting plate, and the other end is located at the root of the first piercing member.

[0089] In this embodiment, the air guide groove 61 can facilitate the sealing cavity to communicate with the atmosphere after the first puncture member punctures, so that the liquid in the sealing cavity can flow out smoothly.

[0090] The embodiment of the present invention further provides a liquid storage and release method for a microfluidic chip. Based on any of the above liquid storage and release devices, the liquid storage and release method comprises:

[0091] The puncture device is driven to move toward the main body so that the first puncture member 3 punctures the sealing film at the first opening 11 , and the second puncture member 4 in the limiting chamber 2 is bent toward the second opening 12 under the restriction of the limiting slope 21 to puncture the sealing film at the second opening 12 .

[0092] It should be noted that the various liquid storage and release method embodiments of the present invention and the various liquid storage and release mechanism embodiments of the present invention are based on the same inventive concept and can therefore achieve the same technical effects. For specific effects, please refer to the above-mentioned system embodiments and will not be repeated here.

[0093] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A liquid storage and release mechanism for a microfluidic chip, characterized in that: It includes a main body and a puncture device; The main body comprises an adjacent sealed cavity (1) and a limiting chamber (2); the sealed cavity (1) comprises a first opening (11) and a second opening (12) whose opening directions are perpendicular to each other; the second opening (12) is in communication with the limiting chamber (2); the first opening (11) and the second opening (12) are sealed with a sealing film; the puncturing device comprises a first puncturing member (3) and a second puncturing member (4); the first puncturing member (3) faces the first opening (11); the second puncturing member (4) penetrates into the limiting chamber (2); a limiting inclined surface (21) is provided on the inner wall of the limiting chamber (2) relative to the second opening (12); When it is necessary to release the fluid in the sealed cavity (1), the puncture device is driven to move toward the main body so that the first puncture member (3) punctures the sealing membrane at the first opening (11), and the second puncture member (4) in the limiting chamber (2) is bent toward the second opening (12) under the restriction of the limiting inclined surface (21) to puncture the sealing membrane at the second opening (12).

2. The liquid storage and release mechanism according to claim 1, characterized in that: The second piercing member (4) comprises a guide portion (41) and a piercing portion (42) which are connected in sequence. A guide groove (22) is provided in the limiting chamber (2). The guide portion (41) passes through the guide groove (22). The guide groove (22) is used to limit the movement of the second piercing member (4) along a preset direction.

3. The liquid storage and release mechanism according to claim 2, characterized in that: The puncture portion (42) is bent towards the sealing cavity (1).

4. The liquid storage and release mechanism according to claim 2, characterized in that: A plate-shaped force-bearing plate (411) is provided at a portion of the guide portion (41) close to the puncture portion (42); the force-bearing plate (411) is used to cooperate with the limiting inclined surface (21) to control the direction in which the puncture portion (42) is deformed so as to puncture the sealing membrane on the second opening (12).

5. The liquid storage and release mechanism according to claim 1, characterized in that: The puncture device further comprises a buckle (5), a buckle position is provided in the limit bin (2), and the buckle (5) cooperates with the buckle position to limit the position of the puncture device.

6. The liquid storage and release mechanism according to claim 5, characterized in that: The locking position comprises a first locking position (23) and a second locking position (24); when the buckle (5) is located at the first locking position (23), the puncturing device does not puncture the sealing film on the first opening (11) and the sealing film on the second opening (12); when the buckle (5) is located at the second locking position (24), the puncturing device punctures the sealing film on the first opening (11) and the sealing film on the second opening (12).

7. The liquid storage and release mechanism according to claim 2, characterized in that: The puncture device further comprises a force-bearing driving member (82), the force-bearing driving member (82) comprising a mounting plate (6) and a driving surface (7) obliquely arranged on the mounting plate (6), the first puncture member (3) and the second puncture member (4) being respectively mounted on a side of the mounting plate (6) away from the driving surface (7), a driving device being arranged on one side of the driving surface (7), the driving device comprising a plunger pump (8) and a driving member (82), the driving member (82) being arranged on a reciprocating rod (81) of the plunger pump (8), the reciprocating rod (81) being parallel to the mounting plate (6), and being moved along its axial direction by the reciprocating rod (81), the driving member (82), the driving surface (7), the guide portion (41) and the guide groove (22) on the reciprocating rod (81) cooperating to drive the puncture device to move along the preset direction; wherein the plunger pump (8) is connected to the microfluidic chip to provide it with power for fluid control.

8. The liquid storage and release mechanism according to claim 1, characterized in that: The bottom of the limiting chamber (2) is connected to a buffer chamber (9); after the puncturing device punctures the first opening (11) and the second opening (12), the fluid in the sealed chamber (1) flows into the buffer chamber (9) via the limiting chamber (2); the buffer chamber (9) is connected to a fluid control unit of the microfluidic chip; the fluid control unit is used to absorb the fluid in the buffer chamber (9) according to instructions.

9. The liquid storage and release mechanism according to claim 1, characterized in that: It comprises a plurality of sealed cavities (1), each of the sealed cavities (1) corresponding to one of the limit bins (2), one of the first piercing members (3) and one of the second piercing members (4), and the depths of the plurality of sealed cavities (1) are different.

10. The liquid storage and release mechanism according to claim 7, characterized in that: A guide bin (10) is also provided on the main body, and a support body (62) is provided on the mounting plate (6); the support body is inserted into the guide bin (10) and cooperates with the guide bin to control the mounting plate (6) to move in a straight line.

11. The liquid storage and release mechanism according to claim 10, characterized in that: The first piercing member is cross-shaped, the mounting plate is provided with an air guide groove (61), one end of the air guide groove (61) is located at the edge of the mounting plate, and the other end is located at the root of the first piercing member.

12. A liquid storage and release method for a microfluidic chip, characterized in that: Based on the liquid storage and release device according to any one of claims 1 to 11, the liquid storage and release method comprises: The puncture device is driven to move toward the main body so that the first puncture member (3) punctures the sealing membrane at the first opening (11), and the second puncture member (4) in the limiting chamber (2) is bent toward the second opening (12) under the restriction of the limiting inclined surface (21) to puncture the sealing membrane at the second opening (12).

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