A real-time monitoring system and method for suspended biological particles in a laboratory

Abstract

The present invention discloses a real-time monitoring system and method for suspended biological particles in a laboratory. The system includes a monitoring chamber, a mounting base, an air inlet driving structure, a movable plug, a temperature and humidity monitoring module, and a temporary storage chamber. A sampling head is installed at the opening of the monitoring chamber, and a number of first slits for gas to pass through are provided on the sampling head. A lifting seat is movably installed at the bottom of the sampling chamber, and a culture dish is placed on the lifting seat. A rigid tube body is installed at the bottom of the sampling chamber, and the rotation drive of the movable plug simultaneously drives the internal closure of the rigid tube body. In the present invention, the sampling environment parameters in the laboratory are monitored in real time. When the parameters are abnormal, the culture dish is lifted, and at the same time, the inside of the rigid tube body is closed, and the sampling cycle is aborted. Then, the culture dish is driven by a displacement drive structure to move into the storage area, so as to continue the next sampling cycle after the sampling environment meets the standard, realizing the effective utilization of the sampling structure and improving the overall sampling utilization rate and sampling efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of laboratory suspended particle monitoring, and specifically relates to a real-time monitoring system and method for laboratory suspended biological particles. Background Art

[0002] In a laboratory, suspended microbial particles can be monitored manually, but manual monitoring is time-consuming and laborious. A microbial sampler can also be used to monitor suspended microbial particles. There are mainly two collection methods for the microbial sampler: the slit collection method and the pinhole method.

[0003] In the slit collection method, the culture dish can rotate evenly for one week in each cycle. The sampled bacterial particles are evenly distributed on the ring of the petri dish through the slit, and the dynamic distribution of environmental bacteria during the sampling process can be obtained according to the distribution of colonies therein.

[0004] When sampling by the pinhole method, the dust-containing bacteria-laden air passes through the micropores at high speed and is evenly impacted on the agar surface in the culture dish. These living microorganisms obtain uniform and sufficient nutrition on the agar surface. During the culture process, a rapid dynamic rehydration process occurs and they grow rapidly.

[0005] However, the foregoing sampling methods lack an automatic monitoring function during formal sampling. For example, Chinese Patent Publication No. CN117273045A discloses a full-automatic laboratory suspended microbial particle monitoring information management method. In this technical solution, environmental parameters such as temperature and humidity are automatically monitored, and an alarm message is issued when the detected data is abnormal. However, in similar technical solutions, only the detected data of environmental parameters and the like are monitored, and there is no reasonable monitoring and processing of the monitoring quality. When the sampling environmental parameters do not meet the standards, using the sampling sample as a reference to continue data analysis may lead to inaccurate data analysis results for suspended microbial particles, and the entry of this abnormal data will also affect the overall analysis of the data set. Summary of the Invention

[0006] Therefore, the present invention provides a real-time monitoring system for laboratory suspended biological particles, effectively solving the technical problems in the prior art that inaccurate data analysis results for suspended microbial particles may be caused by continuing data analysis when the sampling environmental parameters do not meet the standards, and the ineffective utilization of sampling instruments, resulting in waste.

[0007] To solve the above technical problems, the present invention specifically provides the following technical solution: A real-time monitoring system for laboratory suspended biological particles, comprising:

[0008] A monitoring chamber, at the opening of which a sampling head is installed, and a plurality of first slits for gas to pass through are provided on the sampling head;

[0009] The mounting base can be installed in the monitoring cabin in a liftable manner and is located at the bottom of the sampling head. A cylindrical sampling cavity is formed between the mounting base and the sampling head. A lifting seat is movably installed at the bottom inside the sampling cavity, and a culture dish is placed on the lifting seat.

[0010] The air inlet driving structure is connected to the bottom inside the sampling cavity through an air pipe. The air inlet driving structure drives the flow of air to cause the gas to impact on the culture dish through the first slit.

[0011] The movable plug is rotatably installed at the bottom inside the sampling cavity. A rigid pipe body is installed at the bottom of the sampling cavity. The bottom of the movable plug is movably installed inside the rigid pipe body. The sampling cavity, the movable plug, the rigid pipe body, and the air pipe are connected in sequence. When the movable plug rotates, it drives the inside of the rigid pipe body to be closed at the same time.

[0012] The temperature and humidity monitoring module is used to obtain the temperature and humidity parameters of the sampling environment in the laboratory.

[0013] The temporary storage cabin is installed on the side of the monitoring cabin. A displacement driving structure is installed on the side of the monitoring cabin away from the temporary storage cabin.

[0014] In the case of abnormal temperature and humidity parameters, it drives the movable plug to rotate to lift the culture dish, drives the mounting base to descend away from the sampling head, so as to drive the side of the sampling cavity to open and face the storage area of the temporary storage cabin. Driven by the displacement driving structure, the culture dish moves into the storage area.

[0015] Further,

[0016] A cylindrical frame is installed at the bottom of the sampling head. An annular groove is formed inside the cylindrical frame. A rotating plate is movably installed in the annular groove. The rotating plate is in close contact with the bottom of the sampling head. A plurality of second slits are formed on the rotating plate. The positions of the first slits and the second slits correspond one by one.

[0017] A circular sampling port is formed on the sampling head. The positions of the first slits and the second slits are both arranged along the radius direction of the sampling port.

[0018] Further,

[0019] The inner width of the first slit gradually becomes smaller in the direction from top to bottom. The inner width of the second slit is the same up and down, and the width of the second slit is the same as the bottom width of the first slit.

[0020] Further,

[0021] The movable plug includes a connecting cylinder connected to the bottom of the lifting seat, a transmission wheel connected to the outside of the connecting cylinder, and a threaded cylinder connected to the bottom of the mounting seat;

[0022] A ventilation opening facing the connecting cylinder is provided at the central position of the lifting seat. The connecting cylinder penetrates through the central position of the bottom of the lifting seat, and at least part of its outer wall is provided with external threads and is in threaded cooperation with the threaded cylinder;

[0023] A number of support blocks are installed on the lifting seat, and the culture dish is placed on the support blocks.

[0024] Further,

[0025] A sealing ring is installed between the outer wall of the connecting cylinder and the bottom of the lifting seat;

[0026] A rotary drive structure is connected to the outside of the transmission wheel, and the rotary drive structure can drive the transmission wheel to rotate.

[0027] Further,

[0028] The rigid pipe body is slidably installed at the bottom of the connecting cylinder. A connecting plate is installed in the connecting cylinder, a cylinder rod is installed at the bottom of the connecting plate, a fixed valve plate is fixedly installed in the rigid pipe body, and a movable valve plate is movably installed above the fixed valve plate;

[0029] The top end of the movable valve plate is connected to a valve rod. The top end of the valve rod is movably installed in the cylinder rod. A sliding block is installed on the outer wall of the valve rod, and a vertical sliding groove is provided on the inner wall of the cylinder rod. The sliding block is slidably arranged in the vertical sliding groove;

[0030] The fixed valve plate is a semi-circular plate, and the movable valve plate is a sector plate, and the angle of its sector area occupying the circular cross-section of the entire rigid pipe body exceeds 180°;

[0031] Further,

[0032] A transmission column is installed on the lifting seat. A first arc-shaped groove is provided on the rotating plate. The top end of the transmission column extends into the first arc-shaped groove and can slide in the first arc-shaped groove;

[0033] A second arc-shaped groove is provided on the rotating plate. A limiting bolt is installed at the bottom of the inner wall of the sampling head. The bottom of the limiting bolt is slidably arranged in the second arc-shaped groove;

[0034] The central angle corresponding to the first arc-shaped groove is greater than 90°, and the circular angle corresponding to the second arc-shaped groove is less than 90°;

[0035] Further,

[0036] A liftable lifting frame is installed in the temporary storage cabin, and a plurality of pallets are installed on the lifting frame, and a plurality of the storage intervals are formed between the pallets.

[0037] Furthermore,

[0038] An installation plate is installed in the monitoring cabin, and the rigid pipe body is penetrated and installed on the installation plate;

[0039] A cylinder is installed on the installation plate, and the output end of the cylinder is connected to the bottom of the installation seat.

[0040] To solve the above technical problems, the present invention further provides the following technical solution: A real-time monitoring method for a real-time monitoring system of suspended biological particles in a laboratory, specifically including the following steps:

[0041] Sample collection is carried out at equal intervals according to the time series;

[0042] Real-time collect the temperature and humidity parameter data of the sampling environment;

[0043] Analyze whether the temperature and humidity parameter data meets the standard;

[0044] Continue the current sampling cycle or abort the current sampling cycle based on the analysis result of the temperature and humidity parameter data;

[0045] Among them, continuing the current sampling cycle or aborting the current sampling cycle based on the analysis result of the temperature and humidity parameter data specifically includes the following steps:

[0046] When the temperature and humidity parameter data meets the standard, continue the current sampling operation, and terminate the sampling cycle after the sampled air volume meets the standard, push the culture dish out of the sampling chamber, place a new culture dish, and perform the next sampling cycle;

[0047] When the temperature and humidity parameter data does not meet the standard, seal the inside of the rigid pipe body to abort the air extraction operation, abort the current sampling cycle, and start the next sampling cycle after the temperature and humidity parameter data meets the standard.

[0048] The present invention has the following beneficial effects compared with the prior art:

[0049] In the present invention, the sampling environment parameters in the laboratory are monitored in real time, and when the parameters are abnormal, the culture dish is lifted, and at the same time, the inside of the rigid pipe body is sealed, the sampling cycle is aborted, and the culture dish is driven by the displacement drive structure to move into the storage interval, so as to continue the next sampling cycle after the sampling environment meets the standard, realizing the effective utilization of the sampling structure and improving the overall sampling utilization rate and sampling efficiency. Description of the Drawings

[0050] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only exemplary, and for those of ordinary skill in the art, without creative efforts, other implementation drawings can also be obtained according to the provided drawings.

[0051] Figure 1 Schematic diagram of the three-dimensional sectional structure of a real-time monitoring system for suspended biological particles in a laboratory provided by an embodiment of the present invention;

[0052] Figure 2 Schematic diagram of the structure of a real-time monitoring system for suspended biological particles in a laboratory provided by an embodiment of the present invention;

[0053] Figure 3 Schematic diagram of the top view structure of a real-time monitoring system for suspended biological particles in a laboratory provided by an embodiment of the present invention;

[0054] Figure 4 For Figure 3 Schematic diagram of the three-dimensional sectional structure of the monitoring cabin in the A-A direction in

[0055] Figure 5 For Figure 3 Schematic diagram of the sectional structure of the monitoring cabin in the A-A direction in

[0056] Figure 6 For Figure 4 Schematic diagram of the enlarged structure of A in

[0057] Figure 7 For Figure 4 Schematic diagram of the enlarged structure of B in

[0058] Figure 8 For Figure 4 Schematic diagram of the enlarged structure of C in

[0059] Figure 9 Schematic diagram of the three-dimensional sectional view of the internal structure of the monitoring cabin from another perspective in an embodiment of the present invention;

[0060] Figure 10 For Figure 9 Schematic diagram of the structure of the connecting cylinder and the rigid pipe body in

[0061] Figure 11 Schematic diagram of the enlarged structure of D in the figure;

[0062] Figure 12 Schematic diagram of the cross-sectional structure of the first slit and the second slit in an embodiment of the present invention.

[0063] The reference numerals in the figure are respectively represented as follows:

[0064] 1 - Monitoring cabin; 2 - Mounting seat; 3 - Movable plug; 4 - Temporary storage cabin; 5 - Sampling head; 6 - First slit; 7 - Sampling cavity; 8 - Lifting seat; 9 - Petri dish; 10 - Trachea; 11 - Rigid pipe body; 12 - Storage area; 13 - Cylindrical frame; 14 - Ring groove; 15 - Rotating plate; 16 - Second slit; 17 - Sampling port; 18 - Sealing ring; 19 - Transmission column; 20 - First arc groove; 21 - Second arc groove; 22 - Limit bolt; 23 - Lifting frame; 24 - Support plate; 25 - Mounting plate; 26 - Cylinder; 27 - Support block;

[0065] 31 - Connecting cylinder; 32 - Transmission wheel; 33 - Threaded cylinder; 34 - Ventilation opening; 35 - Connecting plate; 36 - Cylinder rod; 37 - Fixed valve plate; 38 - Movable valve plate; 39 - Valve rod; 310 - Sliding block; 311 - Vertical chute. Specific embodiments

[0066] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0067] As Figure 1 , Figure 2 , Figure 3 and Figure 4 shown, the present invention provides a real - time monitoring system for suspended biological particles in a laboratory, which has structures such as a monitoring cabin 1, a mounting seat 2, an air inlet driving structure, a movable plug 3, a temperature and humidity monitoring module, a temporary storage cabin 4, etc.

[0068] A sampling head 5 is installed at the opening of the monitoring cabin 1, and a number of first slits 6 for gas to pass through are provided on the sampling head 5, and the gas impacts downward from the first slits 6.

[0069] The mounting seat 2 is liftably installed in the monitoring cabin 1 and is located at the bottom of the sampling head 5. A cylindrical sampling cavity 7 is formed between the mounting seat 2 and the sampling head 5. A lifting seat 8 is movably installed at the bottom of the sampling cavity 7, and a Petri dish 9 is placed on the lifting seat 8.

[0070] The mounting seat 2 and the sampling head 5 can be disassembled from each other and can also be assembled with each other to form a complete sampling structure. When they are combined to form the sampling cavity 7, a sampling operation can be performed.

[0071] The air inlet driving structure is connected to the bottom inside the sampling chamber 7 through the air pipe 10. The air inlet driving structure drives the air body to flow, so as to prompt the gas to impact on the culture dish 9 through the first slit 6. The air inlet driving structure can be a fan structure or an air inlet pump structure connected to the distal end of the air pipe 10.

[0072] The movable plug 3 is rotatably installed at the bottom inside the sampling chamber 7. A rigid pipe body 11 is installed at the bottom of the sampling chamber 7. The bottom of the movable plug 3 is movably installed inside the rigid pipe body 11. The sampling chamber 7, the movable plug 3, the rigid pipe body 11 and the air pipe 10 are communicated in sequence. When the movable plug 3 rotates and drives, it simultaneously drives the inside of the rigid pipe body 11 to be closed.

[0073] The temperature and humidity monitoring module is used to obtain the temperature and humidity parameters of the sampling environment in the laboratory. In addition to the temperature and humidity monitoring module in the present invention, there is also a main control module. The main control module can receive the temperature and humidity parameter data, and analyze whether the temperature and humidity parameter data meets the standard. In the case of non-compliance, it sends a specific start control signal.

[0074] The temporary storage compartment 4 is installed on the side of the monitoring compartment 1. A displacement driving structure is installed on the side of the monitoring compartment 1 away from the temporary storage compartment 4.

[0075] In the case of abnormal temperature and humidity parameters, the main control module issues a start control signal to drive the movable plug 3 to rotate and lift the culture dish 9, and drive the mounting seat 2 to descend away from the sampling head 5, so as to drive the side of the sampling chamber 7 to open and face the storage area 12 of the temporary storage compartment 4. Driven by the displacement driving structure, the culture dish 9 moves into the storage area 12.

[0076] In the present invention, the sampling environment parameters in the laboratory are monitored in real time. In the case of abnormal parameters, the culture dish 9 is lifted. At the same time, the inside of the rigid pipe body 11 is closed, the sampling cycle is aborted, and the culture dish 9 is driven by the displacement driving structure to move into the storage area 12, so as to continue the next sampling cycle after the sampling environment meets the standard, realizing the effective utilization of the sampling structure and improving the overall sampling utilization rate and sampling efficiency.

[0077] In the present invention, in order to optimize the gas impact speed, the following design is also made. A cylindrical frame 13 is installed at the bottom of the sampling head 5. An annular groove 14 is opened inside the cylindrical frame 13. A rotating plate 15 is movably installed in the annular groove 14. The rotating plate 15 is in close contact with the bottom of the sampling head 5. A plurality of second slits 16 are opened on the rotating plate 15. The positions of the first slit 6 and the second slit 16 correspond one by one. A circular sampling port 17 is opened on the sampling head 5. The positions of the first slit 6 and the second slit 16 are both arranged along the radius direction of the sampling port 17.

[0078] Such as Figure 12As shown, the inner width of the first slit 6 gradually decreases in the direction from top to bottom. The inner width of the second slit 16 is uniform up and down, and the width of the second slit 16 is the same as the bottom width of the first slit 6.

[0079] The gas passes through the first slit 6 and the second slit 16 in sequence and impacts on the culture dish 9. In this process, the gas entering the first slit 6 from the sampling port 17 is already a process of reducing the gas flow area, and the process of the gas entering the second slit 16 from the first slit 6 is also a process of further reducing the gas flow area. During the air flow process, the air flow area gradually decreases, which makes the gas flow velocity further increase, optimizes the impact velocity of the gas, and improves the particle collection speed.

[0080] In the present invention, the first slit 6 and the second slit 16 are independent of each other. In the initial state, the positions of the first slit 6 and the second slit 16 correspond to each other. When the rotating plate 15 rotates, the second slit 16 rotates accordingly and is displaced from the position of the first slit 6. When the positions of the first slit 6 and the second slit 16 are displaced from each other, gas cannot flow through the slit, thus achieving the suspension of sampling at the intake end.

[0081] The movable plug 3 can control the gas flow and also drive the lifting of the lifting seat 8. In the present invention, the movable plug 3 adopts the following preferred embodiments, as Figure 4 、 Figure 5 、 Figure 7 、 Figure 9 As shown, the movable plug 3 includes a connecting cylinder 31 connected to the bottom of the lifting seat 8, a transmission wheel 32 connected to the outside of the connecting cylinder 31, and a threaded cylinder 33 connected to the bottom of the mounting seat 2. A ventilation port 34 facing the connecting cylinder 31 is provided at the center of the lifting seat 8. The connecting cylinder 31 passes through the center of the bottom of the lifting seat 8, and at least part of its outer wall is provided with an external thread and is in threaded cooperation with the threaded cylinder 33. A number of support blocks 27 are installed on the lifting seat 8, and the culture dish 9 is placed on the support blocks 27.

[0082] The design of the support blocks 27 raises the bottom of the culture dish 9 to maintain a certain distance from the top surface of the lifting seat 8, and gas can enter the connecting cylinder 31 at the bottom of the lifting seat 8 through the bottom of the culture dish 9.

[0083] Driven by the intake driving structure, the gas passes through the first slit 6 and the second slit 16 from the outside, drives particles to impact on the culture dish. The gas itself, under the driving action, enters the ventilation port 34 from between the bottom of the culture dish 9 and the support blocks 27, passes through the ventilation port 34 into the connecting cylinder 31 and the hard tube body 11, and then flows towards the intake driving structure side through the trachea 10.

[0084] In the above embodiments, a sealing ring 18 is installed between the outer wall of the connecting cylinder 31 and the bottom of the lifting seat 8 to ensure that the inside of the sampling chamber 7 is in a sealed state during the air flow driving process, so that the gas will not be affected by the air pressure in the gap between the connecting cylinder 31 and the ventilation port 34 during the sampling process.

[0085] A rotary drive structure is connected to the outside of the transmission wheel 32. The rotary drive structure can drive the transmission wheel 32 to rotate. The rotation of the transmission wheel 32 can drive the connecting cylinder 31 to rotate. Since the connecting cylinder 31 is threadedly installed in the threaded cylinder 33, during the process of driving the connecting cylinder 31 to rotate, the connecting cylinder 31 is also gradually driven upward, driving the lifting seat 8 to rise.

[0086] Among them, driving the lifting seat 8 to rise synchronously drives the culture dish 9 to rise. When driving the culture dish 9 to rise, it is to make the bottom of the culture dish 9 higher than the high point of the outer wall of the mounting seat 2. At this time, when the culture dish 9 is pushed towards the temporary storage compartment 4 side, the outer wall of the mounting seat 2 will not block the culture dish 9.

[0087] In the present invention, moving the culture dish 9 into the temporary storage compartment 4 not only requires raising the culture dish 9, but also requires opening the side of the sampling chamber 7. For this purpose, a mounting plate 25 is installed in the monitoring chamber 1, and a cylinder 26 is installed on the mounting plate 25. The output end of the cylinder 26 is connected to the bottom of the mounting seat 2. The drive of the cylinder 26 can drive the mounting seat 2 to descend away from the sampling head 5, so that the side openings of the mounting seat 2 and the sampling head 5 are formed. At this time, the culture dish 9 can be removed from the side of the mounting seat 2.

[0088] In order to be able to drive the inside of the hard tube body 11 to be closed during the rotation of the connecting cylinder 31, the present invention makes the following design, as Figure 10 and Figure 11 shown, the hard tube body 11 is fixedly installed through the mounting plate 25, the hard tube body 11 is slidably installed at the bottom of the connecting cylinder 31, a connecting plate 35 is installed in the connecting cylinder 31, a cylinder rod 36 is installed at the bottom of the connecting plate 35, a fixed valve plate 37 is fixedly installed in the hard tube body 11, and a movable valve plate 38 is movably installed above the fixed valve plate 37;

[0089] The top end of the movable valve plate 38 is connected to a valve rod 39. The top end of the valve rod 39 is movably installed in the cylinder rod 36. A sliding block 310 is installed on the outer wall of the valve rod 39, and a vertical sliding groove 311 is opened on the inner wall of the cylinder rod 36. The sliding block 310 is slidably arranged in the vertical sliding groove 311;

[0090] The fixed valve plate 37 is a semi-circular plate, and the movable valve plate 38 is a sector plate, and the angle of its sector area occupying the entire circular cross-section of the hard tube body exceeds 180°.

[0091] There is a sliding connection between the rigid pipe body 11 and the connecting cylinder 31. Since the rigid pipe body 11 is fixedly connected inside the monitoring cabin 1, only the connecting cylinder 31 is in a movable state inside the monitoring cabin 1. The rotation of the connecting cylinder 31 can drive the rotation of the bottom cylinder rod 36 at its bottom, thereby driving the rotation of the valve rod 39 and driving the rotation of the movable valve plate 38.

[0092] In the initial state, the movable valve plate 38 and the fixed valve plate 37 are on the same side, and gas can flow inside the rigid pipe body 11 through the other side of the movable valve plate 38 and the fixed valve plate 37. During the rotation of the connecting cylinder 31, it also rises a certain distance. The sliding block 310 slides in the vertical chute 311, but the cylinder rod 36 can always drive the rotation of the valve rod 39, thereby driving the movable valve plate 38 to move to the other side of the fixed valve plate 37. At this time, from the cross-section, both sides of the inner pipe of the rigid pipe body 11 are blocked by the movable valve plate 38 and the fixed valve plate 37 respectively, and the air flow cannot pass through, realizing the air flow blockage inside the rigid pipe body 11, and thus aborting the current sampling cycle from the air flow driving end.

[0093] To achieve better sealing, the fixed valve plate 37 is a semi-circular plate, and the movable valve plate 38 is a sector plate, and the angle of its sector area occupying the entire circular cross-section of the rigid pipe body exceeds 180°. The movable valve plate 38 can completely block the side of the fixed valve plate 37 to avoid generating gaps and causing air leakage.

[0094] In addition, during the rotation of the connecting cylinder 31, it can also drive the rotation of the rotating plate 15, thereby driving the closing of the air inlet end. The present invention makes the following designs, as Figure 6 and Figure 8 、 Figure 9 shown, a transmission column 19 is installed on the lifting seat 8, and a first arc-shaped groove 20 is formed on the rotating plate 15. The top end of the transmission column 19 extends into the first arc-shaped groove 20 and can slide in the first arc-shaped groove 20;

[0095] A second arc-shaped groove 21 is formed on the rotating plate 15, and a limiting bolt 22 is installed at the bottom of the inner wall of the sampling head 5. The bottom of the limiting bolt 22 is slidably arranged in the second arc-shaped groove 21;

[0096] The central angle corresponding to the first arc-shaped groove 20 is greater than 90°, and the circular angle corresponding to the second arc-shaped groove 21 is less than 90°.

[0097] In the above embodiment, the connecting tube 31 can drive the lifting seat 8 to rotate, thereby driving the rotating plate 15 to rotate through the transmission column 19, wherein the friction force between the transmission column 19 and the first arc-shaped groove 20 is greater than the sum of the static friction resistance of the rotating plate 15 itself, thereby, the transmission column 19 can drive the rotating plate 15 to move through the first arc-shaped groove 20, and no slippage will occur between the transmission column 19 and the first arc-shaped groove 20 during the movement. The movement of the rotating plate 15 also drives the movement of the second arc-shaped groove 21, driving the limit bolt 22 to gradually move relatively to the end of the second arc-shaped groove 21. At this time, the limit bolt 22 prevents the rotating plate 15 from continuing to rotate, and continuing to rotate the transmission column 19 will cause the transmission column 19 to slide in the first arc-shaped groove 20.

[0098] Since the lifting seat 8 also rises during the rotation process, the transmission column 19 rises accordingly. In order to prevent the transmission column 19 from rising to a certain height and being stopped by the top inner wall of the sampling head 5, the transmission column 19 can be set as a retractable column structure, and a spring is arranged inside the column structure.

[0099] In addition, in the initial state, the limit bolt 22 is already at the other end of the second arc groove 21. In order to avoid the first slit 6 and the second slit 16 from overlapping each other during the rotation of the rotating plate 15, it is necessary to make the central angle corresponding to the second arc groove 21 smaller than the angle between adjacent first slits 6, so as to limit the rotation angle of the rotating plate 15 not to exceed the angle between adjacent first slits 6, and the first slit 6 and the second slit 16 will not overlap after being offset.

[0100] In the present invention, the cylinder 26 can be driven successively with the rotation of the lifting seat 8. The lifting seat 8 is rotated first, and when the rotating plate 15 is driven to move a certain angle, the cylinder 26 is driven to make the mounting seat 2 away from the sampling head 5. This can also prevent the top of the transmission column 19 from being supported on the top inner wall of the sampling head 5.

[0101] To realize the lifting and lowering of the storage area 12, Figure 1 and Figure 4 As shown, a lift frame 23 that can be raised and lowered is installed in the temporary storage cabin 4 , and a plurality of support plates 24 are installed on the lift frame 23 , and a plurality of storage areas 12 are formed between the support plates 24 .

[0102] The lifting frame 23 can drive different storage intervals 12 to face the oblique lower part of the mounting seat 2, so that the idle storage interval 12 faces the oblique lower part of the mounting seat 2. After the mounting seat 2 moves down a certain distance, the side of the mounting seat 2 can face the side of the storage interval 12.

[0103] The displacement driving structure in the present invention can be a boosting manipulator arranged on the side of the monitoring chamber 1. This manipulator can push the culture dish 9 to move leftward into the storage area 12, and can also place a new culture dish 9 on the support block 27.

[0104] In summary, the main implementation process of the present invention is as follows:

[0105] The air inlet driving structure drives the air body to flow. The gas passes through the first slit 6 and the second slit 16 from the outside, driving the particles to impact on the culture dish. The gas itself, under the driving action, enters the ventilation port 34 from between the bottom of the culture dish 9 and the support block 27 in the sampling chamber 7, enters the connecting cylinder 31 and the rigid pipe body 11 through the ventilation port 34, and then flows towards the air inlet driving structure side through the air pipe 10;

[0106] When the temperature and humidity parameters are abnormal, the main control module issues a start control signal to drive the rotation driving structure to drive the transmission wheel 32 to rotate. The rotation of the transmission wheel 32 drives the connecting cylinder 31 to rotate, drives the connecting cylinder 31 to spiral upward, drives the lifting seat 8 to rise and rotate 90°;

[0107] During this process, the inner cylinder rod 36 of the rigid pipe body 11 drives the valve rod 39 to rotate, thereby driving the movable valve plate 38 to move to the other side of the fixed valve plate 37. The two sides of the inner part of the pipe of the rigid pipe body 11 are blocked by the movable valve plate 38 and the fixed valve plate 37 respectively, and the air flow cannot pass through, realizing the air flow blockage inside the rigid pipe body 11, and the current sampling cycle is aborted;

[0108] At the same time, the rotation of the connecting cylinder 31 drives the transmission column 19 to rotate, drives the rotating plate 15 to rotate, and the positions of the first slit 6 and the second slit 16 are staggered, blocking the air flow at the air inlet end;

[0109] During the rotation of the rotating plate 15, the limit bolt 22 gradually moves relatively to the end of the second arc-shaped groove 21. At this time, the limit bolt 22 prevents the continuous rotation of the rotating plate 15, and the transmission column 19 continues to rotate and slides in the first arc-shaped groove 20;

[0110] The driving cylinder 26 moves the mounting seat 2 away from the sampling head 5;

[0111] The culture dish 9 is lifted, and at this time, the culture dish 9 is pushed leftward into the storage area 12 through the displacement driving structure;

[0112] Place the new culture dish 9 on the support block 27, and drive the cylinder 26 to reset. During the reset process, the transmission column 19 gradually enters the first arc-shaped groove 20. Then, the rotation drive structure rotates to drive the lifting seat 8 to move downward in a spiral manner, driving the rotating plate 15 to reset. When the first slit 6 and the second slit 16 coincide during the reset, the limit bolt 22 also just relatively moves to the inner end of the second arc-shaped groove 21. The transmission column 19 continues to rotate, but can no longer drive the rotating plate 15 to rotate. After the transmission column 19 slides a certain angle in the first arc-shaped groove 20, it resets to the initial state. The mounting seat 2 also gradually cooperates with the sampling head 5 up and down to form a sealed sampling chamber 7. At this time, the next sampling cycle can be carried out.

[0113] During the above implementation process, the rotation angle of a single spiral rotation action of the lifting seat 8 is 90°. Among them, the central angle corresponding to the first arc-shaped groove 20 must be greater than 90° to match the rotation angle of the transmission column 19.

[0114] The present invention provides a real-time monitoring method for a real-time monitoring system of suspended biological particles in a laboratory, which specifically includes the following steps:

[0115] Collect samples at equal intervals according to the time series.

[0116] Real-time collect the temperature and humidity parameter data of the sampling environment.

[0117] Analyze whether the temperature and humidity parameter data meet the standards.

[0118] Continue the current sampling cycle or abort the current sampling cycle based on the analysis result of the temperature and humidity parameter data.

[0119] Among them, continuing the current sampling cycle or aborting the current sampling cycle based on the analysis result of the temperature and humidity parameter data specifically includes the following steps:

[0120] Under the condition that the temperature and humidity parameter data meet the standards, continue the current sampling action, and terminate the sampling cycle after the sampled air volume meets the standards. Push the culture dish out of the sampling chamber, place a new culture dish, and carry out the next sampling cycle.

[0121] Under the condition that the temperature and humidity parameter data do not meet the standards, seal the inside of the hard tube body to abort the air extraction action, abort the current sampling cycle, and start the next sampling cycle after the temperature and humidity parameter data meet the standards.

[0122] In the above embodiment, the corresponding sampling start time is recorded for each opened sampling cycle.

[0123] The above embodiments are only exemplary embodiments of the present application and are not used to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art can make various modifications or equivalent replacements within the essence and protection scope of the present application, and such modifications or equivalent replacements should also be regarded as falling within the protection scope of the present application.

Claims

1. A real-time monitoring system for suspended biological particles in a laboratory, characterized in that: have: A monitoring cabin (1) having a sampling head (5) installed at its opening, wherein the sampling head (5) is provided with a plurality of first slits (6) for gas to pass through; A mounting seat (2) is movably mounted in the monitoring cabin (1) and is located at the bottom of the sampling head (5); a cylindrical sampling cavity (7) is formed between the mounting seat (2) and the sampling head (5); a lifting seat (8) is movably mounted at the bottom of the sampling cavity (7); a culture dish (9) is placed on the lifting seat (8); An air inlet drive structure connected to the bottom of the sampling chamber (7) via an air pipe (10), the air inlet drive structure driving the air flow to cause the gas to pass through the first slit (6) and impinge on the culture dish (9); A movable plug (3) is rotatably mounted on the bottom of the sampling chamber (7); a hard tube body (11) is mounted on the bottom of the sampling chamber (7); the bottom of the movable plug (3) is movably mounted in the hard tube body (11); the sampling chamber (7), the movable plug (3), the hard tube body (11) and the air pipe (10) are sequentially connected; the movable plug (3) is driven to rotate and simultaneously drives the interior of the hard tube body (11) to be sealed; Temperature and humidity monitoring module, used to obtain the temperature and humidity parameters of the sampling environment in the laboratory; A temporary storage cabin (4) is installed on the side of the monitoring cabin (1), and a displacement drive structure is installed on a side of the monitoring cabin (1) away from the temporary storage cabin (4); When the temperature and humidity parameters are abnormal, the movable plug (3) is driven to rotate and raise the culture dish (9), and the mounting seat (2) is driven to descend away from the sampling head (5), so as to drive the sampling cavity (7) to open laterally and face the storage area (12) of the temporary storage compartment (4), and the culture dish (9) is moved into the storage area (12) under the drive of the displacement drive structure; A cylindrical frame (13) is installed at the bottom of the sampling head (5), an annular groove (14) is provided inside the cylindrical frame (13), a rotating plate (15) is movably installed in the annular groove (14), the rotating plate (15) is tightly abutted against the bottom of the sampling head (5), a plurality of second slits (16) are provided on the rotating plate (15), and the positions of the first slits (6) and the second slits (16) correspond one to one; The movable plug (3) comprises a connecting tube (31) connected to the bottom of the lifting seat (8), a transmission wheel (32) connected to the outside of the connecting tube (31), and a threaded tube (33) connected to the bottom of the mounting seat (2); The lifting seat (8) has a ventilation opening (34) at the center thereof, which is directly opposite to the connecting tube (31). The connecting tube (31) passes through the center of the bottom of the lifting seat (8), and at least a portion of its outer wall is provided with an external thread, which is threadably matched with the threaded tube (33). A plurality of support blocks (27) are installed on the lifting seat (8), and the culture dish (9) is placed on the support blocks (27); The hard tube body (11) is slidably mounted on the bottom of the connecting tube (31); a connecting plate (35) is mounted in the connecting tube (31); a tube rod (36) is mounted at the bottom of the connecting plate (35); a fixed valve plate (37) is fixedly mounted in the hard tube body (11); and a movable valve plate (38) is movably mounted above the fixed valve plate (37); The top end of the movable valve plate (38) is connected to a valve stem (39), and the top end of the valve stem (39) is movably mounted in the barrel stem (36); A transmission column (19) is installed on the lifting seat (8), a first arc-shaped groove (20) is opened on the rotating plate (15), and the top end of the transmission column (19) extends into the first arc-shaped groove (20) and is able to slide in the first arc-shaped groove (20); The rotating plate (15) is provided with a second arc-shaped groove (21), and a limit bolt (22) is installed at the bottom of the inner wall of the sampling head (5), and the bottom of the limit bolt (22) is slidably arranged in the second arc-shaped groove (21); A mounting plate (25) is installed in the monitoring cabin (1), and the hard tube body (11) is installed through the mounting plate (25); A cylinder (26) is mounted on the mounting plate (25), and an output end of the cylinder (26) is connected to the bottom of the mounting seat (2).

2. The real-time monitoring system for laboratory suspended biological particles according to claim 1, characterized in that: The sampling head (5) is provided with a circular sampling port (17), and the positions of the first slit (6) and the second slit (16) are both arranged along the radial direction of the sampling port (17).

3. The real-time monitoring system for laboratory suspended biological particles according to claim 2, characterized in that: The internal width of the first slit (6) gradually decreases from top to bottom, the internal width of the second slit (16) is consistent from top to bottom, and the width of the second slit (16) is consistent with the bottom width of the first slit (6).

4. The real-time monitoring system for laboratory suspended biological particles according to claim 2, characterized in that: A sealing ring (18) is installed between the outer wall of the connecting tube (31) and the bottom of the lifting seat (8); The transmission wheel (32) is externally connected to a rotation drive structure, and the rotation drive structure can drive the transmission wheel (32) to rotate.

5. The real-time monitoring system for laboratory suspended biological particles according to claim 2, characterized in that: A sliding block (310) is installed on the outer wall of the valve stem (39), a vertical sliding groove (311) is opened on the inner wall of the cylinder rod (36), and the sliding block (310) is slidably arranged in the vertical sliding groove (311); The fixed valve plate (37) is a semicircular plate, and the movable valve plate (38) is a fan-shaped plate, and the angle of the fan-shaped area occupied by the entire circular cross-section of the hard tube body exceeds 180°.

6. The real-time monitoring system for laboratory suspended biological particles according to claim 1, characterized in that: The center angle corresponding to the first arc-shaped groove (20) is greater than 90°, and the circular angle corresponding to the second arc-shaped groove (21) is less than 90°.

7. The real-time monitoring system for laboratory suspended biological particles according to claim 1, characterized in that: A lift frame (23) that can be raised or lowered is installed in the temporary storage cabin (4), a plurality of support plates (24) are installed on the lift frame (23), and a plurality of storage areas (12) are formed between the support plates (24).

8. A real-time monitoring method of a real-time monitoring system for laboratory suspended biological particles according to any one of claims 1 to 7, characterized in that: The specific steps include: Sample collection is carried out according to time series and other cycles; Collect temperature and humidity parameter data of the sampling environment in real time; Analyze whether the temperature and humidity parameter data meet the standards; Continue the current sampling cycle or terminate the current sampling cycle based on the temperature and humidity parameter data analysis results; Wherein, continuing the current sampling cycle or terminating the current sampling cycle based on the temperature and humidity parameter data analysis result specifically includes the following steps: When the temperature and humidity parameter data meet the standards, the current sampling action is continued, and the sampling cycle is terminated after the sampled air volume meets the standards, the culture dish is pushed out of the sampling cavity, and a new culture dish is placed in it to carry out the next sampling cycle; When the temperature and humidity parameter data do not meet the standards, the inside of the hard tube body is closed to stop the air extraction action, the current sampling cycle is terminated, and the next sampling cycle is started after the temperature and humidity parameter data meet the standards.

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