Intelligent simulation method and device for corneal near physiological biomechanics

Abstract

The application discloses a kind of corneal near physiological biomechanics intelligent simulation method and device, it is related to biomechanics and biomechanics experimental device technical field, the present application includes mutually connected force load cavity, force loading component, sensor monitoring feedback component etc..The present application can realize to corneal repair material, corneal cell, corneal cell-material compound loading individual and compound flow shear force, static pressure, dynamic pressure, static tension and dynamic tension, gas-liquid dynamic culture is simultaneously carried out, and through pressure and flow sensor, the stress size of loading is monitored in real time, reach accurate intelligent comprehensive simulation cornea in body environment.The scheme provided by the present application can be used for the mechanical property detection of corneal tissue and material, obtains the material attribute under near physiological state;It can also be used for the mechanical loading of cell, to observe the cell behavior and functional change after near physiological mechanical loading.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biomechanics and biomechanics experiment device, in particular to a cornea near physiological biomechanics intelligent simulation method and device. BACKGROUND

[0002] Cornea is a transparent, avascular, highly innervated tissue, located in the anterior part of the eye, protecting the ocular surface from the outside world by epithelial cells and tear film. Cornea, like a transparent window, provides about two-thirds of the refractive power of the eye. In addition to being an important component of the refractive system, providing protection for the contents of the eye, the human cornea is also a load-bearing tissue with unique biomechanical properties. Mechanical stimulation is an important determinant of cell biology function, which is as important as chemical stimulation in determining tissue fate or pathological state. Human living cells are subjected to various mechanical stimuli throughout their life, and changes in the environment in vitro and physiological conditions in vivo will cause changes in stress and strain. Cells can respond to mechanical stimuli in various ways, depending on the intensity, direction and distribution of the stimuli. The cornea is subjected to a very complex stress environment in vivo. The main sources of stress include: tear film movement, eyelid movement, intraocular pressure, artificial rubbing of the eye and contact lens wearing, etc.; and the main types of stress include: shear stress, compressive stress, tensile stress, etc.

[0003] Due to its location on the outside of the eyeball, the cornea is extremely vulnerable to a variety of injuries. Eye trauma, injury and infection can cause varying degrees of corneal defects and visual impairment. Corneal defects and diseases are the third most common cause of visual impairment worldwide. It is estimated that 4.2 million people worldwide experience visually significant corneal opacity. Corneal blindness has a major impact on quality of life and economic productivity. Corneal transplantation is the only effective treatment for corneal blindness, and the medical community is currently facing the difficult problem of a severe shortage of corneal donors. According to statistics, of every 10 million patients in need of corneal transplantation, 1.5-2 million people are blinded due to the shortage of corneal donations. Corneal replacement materials are ideal new transplantation options. Due to the special nature of the anatomical and physiological structure of the cornea, there are few methods for evaluating corneal replacement materials, and standardization has not yet been achieved.

[0004] The cornea is a complex tissue structure, with varying mechanical properties across different corneal layers. Current mechanical loading devices for corneal tissue suffer from issues such as damage to corneal integrity, limited loading methods, and in vivo differences in loading patterns. Furthermore, while various animal species have been used for corneal research, it is known that corneal tissue structure varies between species. However, there are no unified testing devices or recognized standards for this. Recent research has often limited the evaluation of corneal replacement materials to transparency and biocompatibility. However, the development and progression of various corneal diseases are often accompanied by abnormal changes in the corneal mechanical environment. Furthermore, the mechanical properties of corneal repair materials are crucial for their mechanical strength and tolerance during keratoplasty, as well as their long-term stability and functional recovery after surgery. Currently, most mechanobiological loading methods used in experimental research fail to address the physiological properties of the cornea and remain limited to simple mechanical loading of cells. The significant differences between the evaluation environment for corneal replacement materials and the clinical environment can lead to poor evaluation effectiveness, inadequate evaluation, and even risks in clinical use. Furthermore, understanding the impact of corneal environmental mechanical factors and the mechanical properties of corneal replacement materials on corneal cell morphology and function will help us understand the development and progression of corneal diseases and damage repair. Current mechanical loading methods are limited and cannot simulate the physiological mechanical environment of the cornea. As a result, there is still a lack of systematic understanding of the impact of mechanical factors on corneal cell function and the mechanisms behind this. Furthermore, due to a lack of technology, research on corneal cell-material complexes for corneal damage repair remains limited to observing biocompatibility, lacking an understanding of the interactions between cells and repair materials in a bulk mechanical environment and their mechanisms.

[0005] Therefore, it is necessary to develop a technology that can intelligently simulate the physiological and biomechanical environment of the cornea for the mechanical properties testing of corneal tissues and materials, obtain the mechanical properties of materials under near-physiological conditions, and can also be used for mechanical loading of cells to observe cell behavior and functional changes after near-physiological mechanical loading. Summary of the Invention

[0006] The purpose of the present invention is to provide a method and device for intelligent simulation of corneal near-physiological biomechanics to solve the problems existing in the above-mentioned prior art. The intelligent simulation method and device can be used for mechanical property detection of corneal tissue and materials to obtain material properties under near-physiological conditions; they can also be used for mechanical loading of cells to observe cell behavior and functional changes after near-physiological mechanical loading.

[0007] It is convenient to obtain the material mechanical properties of the cornea under near physiological conditions.

[0008] To achieve the above object, the present invention provides the following solutions:

[0009] The present invention provides a corneal near-physiological biomechanics intelligent simulation method, comprising:

[0010] Constructing test environment: fixing cornea sample in the force loading cavity, which separates the force loading cavity into upper cavity and lower cavity;

[0011] Loading force: injecting fluid into the upper cavity and / or the lower cavity to simulate the real in-vivo mechanical environment by fluid pressure and / or flow force.

[0012] Preferably, injecting fluid into the lower cavity by pressure loading assembly to make the cornea sample bulge towards the upper cavity and form bulge; injecting fluid from one side of the upper cavity and flowing out from the other side of the upper cavity to flow through the bulge by flow shear force loading assembly; detecting the fluid entering the upper cavity by setting flow sensor in the upper cavity; detecting the hydraulic pressure in the lower cavity by setting pressure sensor in the lower cavity.

[0013] Preferably, the circumferential edge of the communication between the upper cavity and the lower cavity is provided with an annular fixing assembly, which tightens and fixes the cornea sample through the fixing assembly.

[0014] Preferably, the fixing assembly comprises annular silicone gasket and clamping block, the annular silicone gasket is fixedly arranged in the force loading cavity, the annular silicone gasket is arranged around the inner wall of the force loading cavity, a plurality of clamping holes or clamping grooves are sequentially arranged on the annular silicone gasket along the circumference, one clamping hole corresponds to one clamping block, the annular silicone gasket is used for laying the cornea sample, and the clamping block is used for clamping into the clamping hole or clamping groove and tightly fixing the cornea sample.

[0015] Preferably, the test device is made of milky white resin.

[0016] Preferably, the flow shear force loading assembly comprises first blue cap bottle and first peristaltic pump, the first blue cap bottle, the first peristaltic pump and the upper cavity are sequentially communicated through pipeline; the first peristaltic pump is used for loading flow shear force on the cornea sample; the pressure loading assembly comprises second blue cap bottle and second peristaltic pump, the second blue cap bottle, the second peristaltic pump and the lower cavity are sequentially communicated through pipeline; and the second peristaltic pump is used for loading liquid expansion pressure on the cornea sample.

[0017] Preferably, the flow sensor, the pressure sensor, the second peristaltic pump and the first peristaltic pump are in communication connection with the control system.

[0018] Preferably, the test device comprises detachable upper box and lower box.

[0019] Preferably, the corneal sample is any one or a combination of multiple species and multiple layers of corneal tissue, corneal repair material, corneal cells, and cell-material composite.

[0020] The application also provides a corneal near-physiological biomechanics intelligent simulation device, comprising:

[0021] A force loading cavity for accommodating and fixing a corneal sample, the corneal sample separating the force loading cavity into an upper cavity and a lower cavity;

[0022] A pressure loading assembly for injecting fluid into the lower cavity to make the pressure in the lower cavity reach a set value, the pressure fluid in the lower cavity enabling the corneal sample to swell towards the upper cavity and form a swelling part;

[0023] A flow shear force loading assembly for enabling fluid to flow from one side of the swelling part to the other side;

[0024] A pressure sensor having a detection end arranged in the lower cavity and used for detecting the fluid pressure in the lower cavity and feeding back to a control system; and

[0025] A flow sensor having a detection end arranged in the upper cavity and used for detecting the flow of fluid into the upper cavity and feeding back to the control system.

[0026] The application has the following technical effects relative to the prior art:

[0027] First, the corneal near-physiological biomechanics intelligent simulation method disclosed by the application can simulate the environment of cornea in the body in multiple dimensions and more accurately. In terms of mechanical environment: the device can adjust the size of the force applied by the device at any time according to the normal range of the intraocular pressure and flow shear force of the measured material experimental body species cornea, and simulate the direction and point of action of the force by using fluid according to the in-vivo stress form such as tear film flow, aqueous humor and other contents.

[0028] Second, the upper half of the upper cavity of the simulation device is in air communication, which is more in line with the gas-liquid environment of the cornea in the body. Therefore, the device can construct a more near-physiological environment during the evaluation of corneal related materials, reduce the errors caused by insufficient simulation, flexibly, accurately and multidirectionally evaluate the physicochemical properties and biological properties of the material, and reduce the risk of subsequent experiments or clinical applications

[0029] Third, the simulation device disclosed in the application adds flow sensors, pressure sensors and other mechanical devices on hardware facilities to achieve precision and better serve the controllability and visibility of in-vitro simulation near physiology. During the evaluation process, the fidelity of the simulation environment can be observed in real time and accurately, or relevant corrections can be made in time. Therefore, the application can simultaneously load static liquid pressure and flow shear force on cells and materials near physiology, and visually adjust the stress size. BRIEF DESCRIPTION OF DRAWINGS

[0030] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0031] Fig. 1 is a structural schematic diagram of a corneal near-physiological biomechanical intelligent simulation method provided by the embodiment of the present application

[0032] Fig. 2 is a structural schematic diagram of a force loading cavity

[0033] Fig. 3 is a top view of the lower cavity

[0034] Fig. 4 is a schematic diagram of an intelligent sensing monitoring feedback assembly

[0035] In the drawings, 1 is a force loading cavity; 2 is a flow shear force loading assembly; 3 is a pressure loading assembly; 4 is an intelligent sensing monitoring feedback assembly; 5 is a control system; 11 is a lower cavity; 12 is an upper cavity; 13 is a transverse inlet; 14 is a transverse outlet; 15 is a longitudinal inlet; 16 is a longitudinal outlet; 17 is a ring-shaped silica gel gasket; 18 is a clamping block; 21 is a first blue cap bottle; 22 is a first peristaltic pump; 31 is a second blue cap bottle; 32 is a second peristaltic pump; 41 is a flow sensor; 42 is a pressure sensor. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some embodiments of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0037] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the present application will be further described in detail in combination with the drawings and specific embodiments.

[0038] The present application provides a corneal near-physiological biomechanical intelligent simulation method, as shown in the accompanying drawings, comprising: Figs. 1-4

[0039] Constructing a test environment: fixing a corneal sample in the force loading cavity 1, which separates the force loading cavity 1 into an upper cavity 12 and a lower cavity 11;

[0040] Loading force: injecting fluid into the upper cavity 12 and / or the lower cavity 11 to simulate the real in-vivo mechanical environment by means of fluid pressure and / or flow force.

[0041] The corneal near-physiological biomechanical intelligent simulation method disclosed by the present application can accurately simulate the in-vivo environment of biological membranes such as corneas in multiple dimensions. In terms of mechanical environment: the present technology can refer to the normal range of intraocular pressure and flow shear force of the measured material experimental body species cornea to adjust the size of the force applied by the device at any time, and then realize the mechanical loading of shear force, pressure, tension or multiple force combinations in the physiological to pathological range, according to the in-vivo stress form such as tear film flow, aqueous humor expansion, and the like. Therefore, the present application can simultaneously load static liquid pressure and flow shear force on cells and materials in a near-physiological manner.

[0042] The device can perform biomechanical detection on corneal tissues of various layers of multiple species and corneal replacement materials, obtain the mechanical properties of corneal tissues and corneal replacement materials, and also can perform mechanical loading on corneal cells and cell-material compounds, study the influence of various forces on corneal cell function and its mechanism under in-vivo environment, and the influence of corneal replacement material mechanics on corneal cells and its repair mechanism.

[0043] The lower cavity 11 is a pressure loading cavity, and the upper cavity 12 is a flow shear force loading cavity. In the embodiment of the present application, the purpose of applying pressure to the biological membrane sample is achieved by filling the lower pressure loading cavity with fluid, while the upper half of the upper flow shear force loading cavity is filled with air, which conforms to the gas-liquid environment of the cornea in the body. Therefore, during the evaluation of corneal related materials, the present application can construct a more near-physiological environment, reduce errors caused by insufficient simulation, flexibly, accurately and multidirectionally evaluate the physicochemical properties and biological properties of the materials, and reduce the risk of subsequent experiments or clinical applications.

[0044] In some embodiments, the pressure loading assembly 3 injects fluid into the lower cavity 11 to make the corneal sample swell towards the upper cavity 12 and form a swelling part; the flow shear force loading assembly 2 injects fluid from one side of the upper cavity 12 and flows out from the other side to flow through the swelling part; the flow sensor 41 is arranged in the upper cavity 12 to detect the fluid entering the upper cavity 12; and the pressure sensor 42 is arranged in the lower cavity 11 to detect the hydraulic pressure in the lower cavity 11. ​

[0045] In some embodiments, the circumferential edge of the communication between the upper cavity 12 and the lower cavity 11 is provided with an annular fixing assembly, and the corneal sample is clamped and fixed by the fixing assembly.

[0046] In this embodiment, the outer wall of the annular fixing assembly is in close contact with the inner wall of the force loading cavity 1, the inner hole of the fixing assembly is covered and closed by the corneal sample, which can divide the force loading cavity 1 into two parts.

[0047] In some embodiments, the fixing assembly includes an annular silica gel gasket 17 and a clamping block 18, the annular silica gel gasket 17 is fixedly arranged in the force loading cavity 1, the annular silica gel gasket 17 is arranged around the inner wall of the force loading cavity 1, a plurality of clamping holes or clamping grooves are sequentially arranged on the annular silica gel gasket 17 along the circumference, one clamping hole corresponds to one clamping block 18, the annular silica gel gasket 17 is used for laying the corneal sample, and the clamping block 18 is used for clamping into the clamping hole or clamping groove and tightly fixing the corneal sample. It can be understood that, during operation, the corneal sample is laid on the annular silica gel gasket 17, and the edges of the corneal sample can cover all the clamping holes, and then the clamping block 18 is tightly pressed into the clamping hole from the upper surface of the corneal sample, so as to realize the clamping by interference fit.

[0048] In this embodiment, the flexible annular silica gel gasket 17 and the flexible clamping block 18 are used to clamp and fix the edge of the corneal sample, which can avoid damage to the corneal sample, and the clamping method is convenient for installing and dismounting the corneal sample.

[0049] In addition, because there are a plurality of clamping blocks 18, and the clamping block 18 is small in size, in order to avoid losing the clamping block 18, the plurality of clamping blocks 18 are sequentially connected and integrally formed as an annular structure, and the distance between adjacent clamping blocks 18 is consistent with the distance between adjacent clamping holes, so as to facilitate one-to-one correspondence between the clamping block 18 and the clamping hole.

[0050] The width of the silica gel gasket in the above embodiments can be selected as 20mm, and the thickness can be selected as 1.8mm. A plurality of through holes, i.e. clamping holes, are uniformly distributed on the outer edge of the gasket, and the hole diameter is 1mm. The clamping block 18 is 1mm in diameter and 1mm in thickness.

[0051] In some embodiments, a test device is included, and the force loading cavity 1 is formed in the test device. The test device is made of milky white resin.

[0052] In order to facilitate the dismounting of the corneal sample, in some embodiments, the test device includes a detachable upper box and a lower box.

[0053] Specifically, the upper box bottom edge is provided with a ring-shaped boss made of silica gel, and the lower box top edge is provided with a ring-shaped groove with silica gel padding, and the groove and the boss are combined through the key groove to realize the close connection and disassembly function, of course, it is not limited to this connection mode, as long as the existing technology can be selected to detachably connect and seal the two opposite opening boxes, it can be applied to the present application.

[0054] In some embodiments, the flow shear force loading assembly 2 comprises a first blue cap bottle 21 and a first peristaltic pump 22, and the first blue cap bottle 21, the first peristaltic pump 22 and the upper cavity 12 are sequentially communicated through pipelines. The pressure loading assembly 3 comprises a second blue cap bottle 31 and a second peristaltic pump 32, and the second blue cap bottle 31, the second peristaltic pump 32 and the lower cavity 11 are sequentially communicated through pipelines.

[0055] The fluid speed can be adjusted according to the detection object to realize the mechanical loading of shear force, pressure, tension or multiple forces in the physiological to pathological range

[0056] In this embodiment, the flow shear force is simulated by controlling the rotation speed of the first peristaltic pump 22 to adjust the flow speed. Here, the parallel flat plate flow cavity shear stress formula is referred to:

[0057] τ=6μQ / Wh 2 (1)

[0058] Wherein the unit of τ is dyn / cm 2 , wherein μ is the viscosity coefficient of the perfusion fluid; W is approximately the diameter of the corneal sample; h is the height from the center of the corneal sample to the edge of the bulge; Q is the flow rate determined by the rotation speed n of the first peristaltic pump. Since the flow shear force on the cornea under physiological conditions is a range value rather than an exact value due to tear film movement, eye rubbing, etc., this formula can be used for simulation.

[0059] The static liquid pressure is loaded by the action of the water stop clamp and the second peristaltic pump 32.

[0060] In some embodiments, the fluid outlet of the upper cavity 12 is communicated with the first blue cap bottle 21 to form a circulation loop. The fluid outlet of the lower cavity 11 is communicated with the second blue cap bottle 31 to form a circulation loop.

[0061] This embodiment recycles the fluid, thereby saving the test and detection cost.

[0062] In some embodiments, the flow sensor 41, the pressure sensor 42, the second peristaltic pump 32 and the first peristaltic pump 22 are in communication connection with the control system 5. The flow sensor 41 and the pressure sensor 42 are led out of the force loading cavity 1 to realize external visualization and directly present the related parameters, which is convenient for monitoring and timely adjustment.

[0063] The embodiment realizes intelligent and systematic control. Specifically, the flow sensor 41 and the pressure sensor 42 are used to transmit the detected information to the control system 5, and the control system 5 controls the second peristaltic pump 32 and the first peristaltic pump 22 according to the flow sensor 41, the pressure sensor 42 and the set target, that is, increases the flow, reduces the flow or stops the fluid supply and the like.

[0064] In some embodiments, the sample is any one or a combination of multiple species and multiple levels of corneal tissue, corneal repair material, corneal cells, and cell-material composite.

[0065] In some embodiments, the lower cavity 11 has a vertical inlet and outlet, which are the longitudinal outlet 16 and the longitudinal inlet 15, respectively.

[0066] The upper cavity 12 has a transverse inlet and outlet, which are the transverse outlet 14 and the transverse inlet 13, respectively.

[0067] The working principle of the present application is as follows: before use, the whole device is placed in a high-pressure sterilization pot for sterilization treatment; the test device and the material to be tested (corneal sample) are placed on the super-clean bench, and the edge of the corneal sample is clamped on the annular silica gel gasket 17 through the clamping block 18. Then the extension connectors of the liquid longitudinal inlet 15 and the liquid longitudinal outlet 16 are connected with the second peristaltic pump 32 and the second blue cap bottle 31 through the peristaltic pump pipe, respectively, and the water stop clamp is clamped, and under the output action of the second peristaltic pump 32, the hydrostatic pressure is controlled, the swelling pressure is loaded, and the corneal sample is swelled upward. Subsequently, the extension connectors of the liquid transverse inlet 13 and the liquid transverse outlet 14 are connected with the first peristaltic pump 22 and the first blue cap bottle 21 through the peristaltic pump pipe, respectively, and the culture medium (fluid) in the first blue cap bottle 21 is injected from the fluid transverse inlet 13, flows through the upper surface of the corneal sample, and then flows out from the fluid transverse outlet 14 and circulates. After the combined force loading is realized, the feedback results of the intelligent sensing monitoring feedback assembly 4 and the control system 5 are interacted with the interface to intelligently and systematically control and monitor the whole device.

[0068] After use, the professional personnel separates the upper box and the lower box, then rinses the whole device with clean water, and then the device can be used again after drying and high-pressure sterilization.

[0069] The present application also provides a corneal near-physiological biomechanical intelligent simulation method, which comprises a force loading cavity 1, a pressure loading assembly 3, a flow shear force loading assembly 2, a pressure sensor 42 and a flow sensor 41.

[0070] The force loading cavity 1 is used for accommodating and fixing the corneal sample, the corneal sample divides the force loading cavity 1 into an upper cavity 12 and a lower cavity 11, the pressure loading assembly 3 is used for injecting fluid into the lower cavity 11 to make the pressure in the lower cavity 11 reach a set value, the pressure fluid in the lower cavity 11 can make the corneal sample swell towards the upper cavity 12 and form a swelling part, the flow shear force loading assembly 2 is used for making the fluid flow from one side of the swelling part to the other side, the detection end of the pressure sensor 42 is arranged in the lower cavity 11 and is used for detecting the fluid pressure in the lower cavity 11 and then feeding back to the control system 5, and the detection end of the flow sensor 41 is arranged in the upper cavity 12 and is used for detecting the fluid flow through the swelling part and then feeding back to the control system 5.

[0071] The embodiment of the present application has all the advantages of the above-mentioned corneal near-physiological biomechanical intelligent simulation method, and will not be described here.

[0072] The principle and implementation manner of the present application are described by using specific examples in the present application, and the above embodiment description is only used to help understand the method of the present application and its core idea; meanwhile, for the general technical personnel in the field, according to the idea of the present application, the specific implementation manner and application range will be changed. Therefore, the content of the present description should not be understood as the limitation of the present application.

[0073] The principle and implementation manner of the present application are described by using specific examples in the present application, and the above embodiment description is only used to help understand the method of the present application and its core idea; meanwhile, for the general technical personnel in the field, according to the idea of the present application, the specific implementation manner and application range will be changed. Therefore, the content of the present description should not be understood as the limitation of the present application.

Claims

1. A corneal near-physiological biomechanics intelligent simulation method, characterized by: include: Constructing a test environment: fixing a corneal sample in the force loading chamber, wherein the corneal sample divides the force loading chamber into an upper chamber and a lower chamber; Loading force: injecting fluid into the lower chamber and the upper chamber to simulate the real in vivo mechanical environment by means of fluid pressure and flow force; injecting fluid into the lower chamber through a pressure loading component to cause the corneal sample to bulge toward the upper chamber and form a bulging portion; The fluid is injected from one side of the upper cavity by a flow shear force loading component and flows out from the other side of the upper cavity to flow through the bulge portion; and a flow sensor is arranged in the upper cavity to detect the fluid entering the upper cavity; A pressure sensor is arranged in the lower chamber to detect the hydraulic pressure in the lower chamber; an annular fixing component is arranged on the circumferential edge of the connection between the upper chamber and the lower chamber, and the corneal sample is tensioned and fixed by the fixing component; the fixing component includes an annular silicone gasket and a clamping block, the annular silicone gasket is fixedly arranged in the force load chamber, the annular silicone gasket is arranged around the inner wall of the force load chamber, and a plurality of bayonet holes or slots are sequentially opened on the annular silicone gasket along the circumference, one bayonet hole corresponds to one clamping block, the annular silicone gasket is used to lay the corneal sample, and the clamping block is used to be clamped into the bayonet hole or slot and The corneal sample is pressed and fixed; the corneal sample is corneal tissue; when the fluid is injected into the upper chamber, the initial flow direction is horizontal; the pressure loading component and the flow shear force loading component cooperate to simulate the force environment of the corneal sample on the in vivo ocular surface flow shear force; the pressure sensor can feed back the fluid pressure information in the lower chamber to the control system; the flow sensor can feed back the fluid flow information in the upper chamber to the control system; the control system adjusts the pressure loading component and the flow shear force loading component according to the fluid pressure information and the fluid flow information to adjust the fluid pressure and fluid shear force in the lower chamber and the upper chamber in real time.

2. The corneal near-physiological biomechanics intelligent simulation method according to claim 1, characterized in that: The test device comprises a test device, in which the force load cavity is formed, and the test device is made of milky white resin.

3. The corneal near-physiological biomechanics intelligent simulation method according to claim 1, characterized in that: The flow shear force loading component includes a first blue-capped bottle and a first peristaltic pump, and the first blue-capped bottle, the first peristaltic pump and the upper chamber are connected in sequence through a pipeline; the flow shear force is loaded on the corneal sample through the first peristaltic pump; the pressure loading component includes a second blue-capped bottle and a second peristaltic pump, and the second blue-capped bottle, the second peristaltic pump and the lower chamber are connected in sequence through a pipeline; the liquid expansion pressure is loaded on the corneal sample through the second peristaltic pump.

4. The corneal near-physiological biomechanics intelligent simulation method according to claim 3, characterized in that: The flow sensor, the pressure sensor, the second peristaltic pump, and the first peristaltic pump are communicatively connected to a control system.

5. The corneal near-physiological biomechanics intelligent simulation method according to claim 2, characterized in that: The test device comprises a detachable upper box and a lower box.

6. A corneal near-physiological biomechanics intelligent simulation device, characterized by: The method for realizing the corneal near-physiological biomechanics intelligent simulation method according to any one of claims 1 to 5 comprises: A force-bearing chamber, used for accommodating and fixing a corneal sample, wherein the corneal sample divides the force-bearing chamber into an upper chamber and a lower chamber; a pressure loading component, configured to inject fluid into the lower chamber to make the pressure in the lower chamber reach a set value, wherein the pressure fluid in the lower chamber can cause the corneal sample to swell toward the upper chamber and form a bulging portion; a flow shear force loading component, used for causing the fluid to flow from one side of the expansion portion to the other side; a pressure sensor, whose detection end is placed in the lower chamber and is used to detect the fluid pressure in the lower chamber and then feed back to the control system; and The flow sensor has a detection end placed in the upper cavity and is used to detect the flow of the fluid entering the upper cavity and then feed back to the control system.

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