Plant growth regulator for preventing cut rose flower nectar and preparation method thereof

Abstract

The present invention relates to the technical field of rose cultivation. Provided are a plant growth regulator for preventing the occurrence of cut rose flower necrosis and a preparation method. The raw materials of the plant growth regulator include: naphthylacetic acid mother solution, cytokinin mother solution, fluridone mother solution, cyprodinil mother solution and surfactant. The plant growth regulator provided by the present invention can effectively promote the growth rate of rose receptacles and cell division, regulate the growth and development of apical meristems, thereby greatly reducing the incidence of cut rose flower necrosis and improving product quality. In addition, the plant growth regulator provided by the present invention has a simple preparation process, rapid effect, low cost, environmental protection and safety, and obvious effect, and can effectively shorten the receptacle length, reduce the number of petals, and improve the quality of cut flowers.

Description

Technical Field

[0001] The invention relates to the technical field of cut rose cultivation, and in particular to a plant growth regulator for preventing cut rose flower nectar from occurring and a preparation method thereof. Background Art

[0002] Rose (Rosa hybrida), Rosaceae, is a perennial evergreen or semi-evergreen low shrub that blooms all year round with rich colors and varieties. It can be used as an ornamental and medicinal plant and is also widely used in cut flowers. As one of the four major cut flowers in the world, rose has a high economic value and is one of the most consumed flowers in the cut flower market. High-quality cut flowers require not only the appropriate length of branches, but also the flower shape, shape, and degree of opening are also one of the indicators for judging its grade and quality.

[0003] During the production process of cut roses, the mainstream varieties on the market (such as Pink Snow Mountain, Peach Snow Mountain, and Candy Snow Mountain in the Snow Mountain series) have lost their commercial value and seriously reduced their quality and commercial value due to the abnormal development of the receptacle (showing an obvious epistasis phenomenon) and the increase in the number of petals caused by the low temperature in winter, which leads to the inability to open normally.

[0004] Therefore, exploring methods that can effectively prevent the occurrence of cut rose necrosis has extremely important industrial significance. Summary of the invention

[0005] The purpose of the present invention is to solve the defects of the above-mentioned prior art and provide a plant growth regulator for preventing the occurrence of cut rose flower necrosis and a preparation method thereof.

[0006] A plant growth regulator for preventing the occurrence of cut rose flower necrosis, the raw materials of which include:

[0007] Naphthaleneacetic acid mother solution, cytokinin mother solution, fluridone mother solution, cyproconazole mother solution and surfactant.

[0008] Further, the plant growth regulator for preventing the occurrence of cut rose necrosis as described above,

[0009] The naphthylacetic acid mother solution is prepared by adding 5 mL of 70% anhydrous ethanol to 1 g of naphthylacetic acid, and then adding water to make the volume to 1 L after the naphthylacetic acid is fully dissolved;

[0010] The cytokinin mother solution is prepared by adding 5 mL of 1 mol / L sodium hydroxide solution to 1 g of cytokinin, and then adding water to make the volume to 1 L after the solution is fully dissolved.

[0011] The fluoropyridone mother solution is prepared by adding 5 mL of DMSO to 1 g of fluoropyridone to fully dissolve it and then adding 995 mL of water;

[0012] The cyproconazole mother solution is prepared by adding 1L of water to 1g of cyproconazole.

[0013] Furthermore, in the plant growth regulator for preventing the occurrence of cut rose flower necrosis as described above, the volume ratio of the naphthylacetic acid mother solution, the cytokinin mother solution, the fluridone mother solution, the cyproconazole mother solution and the water is (93-931):(113-1126):82:5:50000.

[0014] The method for preparing the plant growth regulator for preventing the occurrence of cut rose flower necrosis as described above comprises the following steps:

[0015] Step 1: respectively prepare naphthylacetic acid mother solution, cytokinin mother solution, fluridone mother solution, and cyproconazole mother solution;

[0016] Step 2: Add the naphthylacetic acid mother solution, cytokinin mother solution, fluridone mother solution, and cyprodinil mother solution into water, stir evenly, and obtain a mixed solution;

[0017] Step 3: Add a surfactant to the mixed solution, shake and mix thoroughly, and then adjust the pH of the mixed solution to 5.8-6.0, to finally obtain the plant growth regulator for preventing the occurrence of cut rose flower necrosis.

[0018] The use of the plant growth regulator for preventing the occurrence of cut rose necrosis as described above in preventing the occurrence of cut rose necrosis.

[0019] The application as described above comprises: spraying the flower buds of the cut rose plants that are about to complete flower bud differentiation with the plant growth regulator composition for preventing the occurrence of cut rose flower buds, wherein the diameter of the flower buds is 0.8-1.2 cm;

[0020] The spraying time is from 16:00 to 18:00 on a sunny afternoon. The plants should be kept dry during spraying to avoid spraying in high temperatures at noon.

[0021] The plant growth regulator for preventing the occurrence of cut rose flower necrosis provided by the present invention promotes the elongation growth of cells through naphthaleneacetic acid, regulates the growth rate of the receptacle, and promotes the division and differentiation of flower organs through cytokinins. Through the combination of the two, the growth rate of the rose receptacle and the division of cells can be effectively promoted, and the growth and development of the apical meristem can be regulated, thereby greatly reducing the incidence of cut rose flower necrosis and improving product quality. In addition, the plant growth regulator for preventing the occurrence of cut rose flower necrosis provided by the present invention has a simple preparation process, rapid effect, low cost, environmental protection and safety, and obvious effect, and can effectively shorten the length of the receptacle, reduce the number of petals, and improve the quality of cut flowers.

[0022] In addition, the present invention can make the plant growth regulator for preventing the occurrence of cut rose flower buds have better stability by controlling the pH value of the regulator at 5.8-6.0. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a comparison diagram of the flower diameter length between the control group and the treatment group 1 of the present invention;

[0024] Figure 2 This is a comparison diagram of the flower heads of the control group and treatment group 1 of the present invention;

[0025] Figure 3 This is a comparison diagram of the receptacles of the control group and treatment group 1 of the present invention;

[0026] Figure 4 This is a side view comparison of flowers of the control group 1 and the treatment groups 1-9 that have reached commercial grade;

[0027] Figure 5 This is a top view comparison of commercial grade flowers of the control group 1 and the treatment groups 1-9 of the present invention. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The cyprodinil used in the present invention was purchased from Syngenta·Sedford, and the pesticide registration certificate number is PD20120252. The surfactant used is L-77, which was purchased from Yisheng Biological Silwet-L77, and the product number is No. S9430.

[0030] Embodiment 1:

[0031] (1) Add 5 mL of 70% anhydrous ethanol to 1 g of naphthaleneacetic acid, wait for it to be fully dissolved, and then add water to make up the volume to 1 L to prepare a 1 g / L naphthaleneacetic acid mother solution; add 5 mL of 1 mol / L sodium hydroxide solution to 1 g of cytokinin, wait for it to be fully dissolved, and then add water to make up the volume to 1 L to prepare a 1 g / L cytokinin mother solution; add 5 mL of DMSO to 1 g of fluridone, wait for it to be fully dissolved, and then add 995 mL of water to prepare a 1 g / L fluridone mother solution; add 1 L of water to 1 g of cyproconazole to prepare a 1 g / L cyprodinil mother solution.

[0032] (2) Add 0.93 mL of naphthaleneacetic acid mother solution, 1.126 mL of cytokinin mother solution, 0.82 mL of fluridone mother solution, 50 μL of surfactant L-77, and 50 μL of cyproconazole mother solution to 500 mL of water, shake and mix thoroughly, adjust the pH of the mixture to 5.8 after mixing evenly, and obtain a plant growth regulator 1 for preventing the occurrence of cut rose flower necrosis.

[0033] Embodiment 2:

[0034] The difference between this embodiment and embodiment 1 is that: 0.93 mL of the mother solution of naphthylacetic acid, 5.63 mL of the mother solution of cytokinin, 0.82 mL of the mother solution of fluridone, 50 μL of a surfactant, and 50 μL of cyproconazole and fludioxonil are added to 500 mL of water, and the mixture is shaken and mixed thoroughly, and the pH of the mixture is adjusted to 5.9 after being uniformly mixed, so as to obtain plant growth regulator 2 for preventing the occurrence of cut rose flower necrosis.

[0035] Embodiment 3:

[0036] The difference between this embodiment and embodiment 1 is that: 0.93 mL of the mother solution of naphthylacetic acid, 11.26 mL of the mother solution of cytokinin, 0.82 mL of the mother solution of fluridone, 50 μL of a surfactant, and 50 μL of cyproconazole were added to 500 mL of water, and the mixture was shaken and mixed thoroughly. After the mixture was evenly mixed, the pH of the mixture was adjusted to 6.0, thereby obtaining a plant growth regulator 3 for preventing the occurrence of cut rose flower necrosis.

[0037] Embodiment 4:

[0038] The difference between this embodiment and embodiment 1 is that: 4.66 mL of naphthaleneacetic acid mother solution, 1.126 mL of cytokinin mother solution, 0.82 mL of fluridone mother solution, 50 μL of surfactant L-77, and 50 μL of cyproconazole mother solution were added to 500 mL of water, and the mixture was fully shaken and mixed, and the pH of the mixture was adjusted to 6.0 after uniformity, so as to obtain plant growth regulator 4 for preventing the occurrence of cut rose flower necrosis.

[0039] Embodiment 5:

[0040] The difference between this embodiment and embodiment 1 is that: 4.66 mL of the mother solution of naphthylacetic acid, 5.63 mL of the mother solution of cytokinin, 0.82 mL of the mother solution of fluridone, 50 μL of the surfactant L-77, and 50 μL of cyproconazole were added to 500 mL of water, and the mixture was fully shaken and mixed, and the pH of the mixture was adjusted to 6.0 after uniformity, so as to obtain a plant growth regulator 5 for preventing the occurrence of cut rose flower necrosis.

[0041] Embodiment 6:

[0042] The difference between this embodiment and embodiment 1 is that 4.66 mL of naphthylacetic acid mother solution, 11.26 mL of cytokinin mother solution, 0.82 mL of fluridone mother solution, 50 μL of surfactant, and 50 μL of cyproconazole mother solution are added to 500 mL of water, and the mixture is fully shaken and mixed, and the pH of the mixture is adjusted to 6.0 after uniformity, so as to obtain plant growth regulator 6 for preventing the occurrence of cut rose flower necrosis.

[0043] Embodiment 7:

[0044] The difference between this embodiment and embodiment 1 is that 9.31 mL of naphthylacetic acid mother solution, 1.126 mL of cytokinin mother solution, 0.82 mL of fluridone mother solution, 50 μL of surfactant, and 50 μL of cyproconazole mother solution are added to 500 mL of water, and the mixture is shaken and mixed thoroughly. After the mixture is evenly mixed, the pH of the mixture is adjusted to 6.0, thereby obtaining plant growth regulator 7 for preventing the occurrence of cut rose flower necrosis.

[0045] Embodiment 8:

[0046] The difference between this embodiment and embodiment 1 is that 9.31 mL of naphthylacetic acid mother liquor, 5.63 mL of cytokinin mother liquor, 0.82 mL of fluridone mother liquor, 50 μL of surfactant L-77, and 50 μL of cyproconazole mother liquor were added to 500 mL of water, and the mixture was fully shaken and mixed, and the pH of the mixture was adjusted to 5.8 after uniform mixing, so as to obtain plant growth regulator 8 for preventing the occurrence of cut rose flower necrosis.

[0047] Embodiment 9:

[0048] The difference between this embodiment and embodiment 1 is that 9.31 mL of naphthylacetic acid mother liquor, 11.26 mL of cytokinin mother liquor, 0.82 mL of fluridone mother liquor, 50 μL of surfactant L-77, and 50 μL of cyproconazole were added to 500 mL of water, and the mixture was fully shaken and mixed, and the pH of the mixture was adjusted to 5.9 after uniformity, so as to obtain plant growth regulator 9 for preventing the occurrence of cut rose flower necrosis.

[0049] Comparative Example 1:

[0050] The difference between this comparative example and Example 1 is that only 0.82 mL of the mother solution of fluridone, 50 μL of the surfactant L-77, and 50 μL of cyproconazole were added to 500 mL of water, and the mixture was fully shaken and mixed to obtain the plant growth regulator of the control group.

[0051] Application example: Use of plant growth regulators to prevent cut rose flower necrosis

[0052] At 17:00 on a sunny afternoon, when the plants are dry, the rose plants that have just entered the apical seed stage (flower bud differentiation is completed to form small buds visible to the naked eye) are sprayed with a plant growth regulator composition to prevent the occurrence of cut rose flower every other week. At other times, conventional production technology management is carried out under the same facilities (intelligent multi-span greenhouse, daytime temperature 25-30°C; nighttime temperature 15-20°C), the same soilless cultivation trough (the matrix is ​​coconut bran and perlite, 3:1), the same water and fertilizer (EC=1.5, PH=5.8-6.0, keeping the relative humidity of the soil at 60%, watering times 8-12 times, each time 90s, and the number of fertilizer watering times is adjusted according to weather conditions), and the pests and diseases (red spider, aphids, thrips, powdery mildew, gray mold and downy mildew) are prevented and controlled once every 7 days.

[0053] test:

[0054] The cut rose 'Pink Snow Mountain' from Baofeng Base of the Institute of Flowers and Plants of the Academy of Agricultural Sciences in Jinning District, Kunming City, Yunnan Province was used as the experimental material. 150 'Pink Snow Mountain' plants at the top seed stage with basically the same size and quality were selected and divided into 10 groups, 15 in each group, and marked. The groups are as follows:

[0055] Treatment group 1: the plant growth regulator prepared in Example 1 was selected; the spraying method was the method of the application example.

[0056] Treatment group 2: the plant growth regulator prepared in Example 2 was selected; the spraying method was the method of the application example.

[0057] Treatment group 3: the plant growth regulator prepared in Example 3 was selected; the spraying method was the method of the application example.

[0058] Treatment group 4: the plant growth regulator prepared in Example 4 was selected; the spraying method was the method of the application example.

[0059] Treatment group 5: the plant growth regulator prepared in Example 5 was selected; the spraying method was the method of the application example.

[0060] Treatment group 6: the plant growth regulator prepared in Example 6 was selected; the spraying method was the method of the application example.

[0061] Treatment group 7: the plant growth regulator prepared in Example 7 was selected; the spraying method was the method of the application example.

[0062] Treatment group 8: The plant growth regulator prepared in Example 8 was selected; the spraying method was selected from the method in the application example.

[0063] Treatment group 9: the plant growth regulator prepared in Example 9 was selected; the spraying method was the method of the application example.

[0064] Control group 1: Comparative Example 1 was sprayed, and the spraying method was the same as that of the application example.

[0065] The first spraying time for each group was on the afternoon of January 8, 2024, when the weather was cloudy. The last pruning was done on February 22, 2024, for a total of 3 sprayings. The probability of occurrence of rose flower in treatment groups 1-9 and control group 1 was statistically analyzed. The results are as follows: Figure 1-Figure 5 , as shown in Table 1.

[0066] Table 1

[0067] Experimental observation varieties The incidence of phthisis Treatment Group 1 Pink Snow Mountain 45％ Treatment Group 2 Pink Snow Mountain 40％ Treatment Group 3 Pink Snow Mountain 40％ Treatment Group 4 Pink Snow Mountain 35％ Treatment Group 5 Pink Snow Mountain 30％ Treatment Group 6 Pink Snow Mountain 25％ Treatment Group 7 Pink Snow Mountain 18％ Treatment Group 8 Pink Snow Mountain 15％ Treatment Group 9 Pink Snow Mountain 8％ Control group 1 Pink Snow Mountain 72％

[0068] As shown in Table 1: The plants in the treatment group were treated on January 8, 2024, and a large number of plants reached the peak flowering period on February 18-20, 2024; the plants in the control group were treated at the same time and reached the peak flowering period on February 18-20, 2024.

[0069] The overall probability of the occurrence of the rose flower in treatment group 1 was 45%; the overall probability of the occurrence of the rose flower in treatment group 2 was 40%, the overall probability of the occurrence of the rose flower in treatment group 3 was 40%, the overall probability of the occurrence of the rose flower in treatment group 4 was 35%, the overall probability of the occurrence of the rose flower in treatment group 5 was 30%, the overall probability of the occurrence of the rose flower in treatment group 6 was 25%, the overall probability of the occurrence of the rose flower in treatment group 7 was 18%, and the overall probability of the occurrence of the rose flower in treatment group 8 was 1 5%, the overall probability of stupid flower occurrence of rose plants in treatment group 9 was 8%, while the overall probability of stupid flower occurrence of rose plants in the control group was 72%. Therefore, the treatment plants can obviously prevent the occurrence of stupid flower in cut roses. It can be seen that the plant growth regulator composition for preventing the occurrence of stupid flower in cut roses prepared by the present invention is sprayed on varieties that are prone to "stupid flower" in winter production of cut roses, and only a few sprays are needed to effectively solve the industrial problems of reduced commodity value such as deformed receptacles and increased number of petals that cannot open normally in the 'Xueshan' series of roses.

[0070] from Figure 1It can be seen that the flower shape of the control group 1 observed from the side view is larger than that of the treatment group 1; the whole flower bud of the control group 1 observed from the top view is tightly wrapped and the petals cannot open normally, while the whole flower bud of the treatment group 1 can open normally; the receptacle of the control group 1 is obviously raised, while the receptacle of the treatment group 1 is in a normal horizontal state. Compared with the control group and the treatment group, the plant growth regulator provided by the present invention for preventing the occurrence of cut rose flower necrosis can make the flower shape normal, the petals open normally, and the receptacle not in place, but in a normal horizontal state. Figure 2 It can be seen that the use of the plant growth regulator provided by the present invention enables the cut rose to grow normally. Figure 3 It can be seen that the plant growth regulator for preventing the occurrence of cut rose flower necrosis provided by the present invention can make the receptacle of the cut rose grow normally.

[0071] pass Figure 4 , Figure 5 It can be seen that the control group has large flower heads and tightly wrapped petals that cannot be opened normally; the plant growth regulators 1-8 provided by the present invention all have a certain phenomenon that the petals cannot be opened, and the plant growth regulator 9 provided by the present invention for preventing the occurrence of cut rose flower necrosis has the best effect after treatment, the flower head size is appropriate, the petals can be opened normally, and it is a normal state of cut roses.

[0072] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A plant growth regulator for preventing the occurrence of cut rose flower necrosis, characterized in that: Its raw materials include: Naphthaleneacetic acid mother solution, cytokinin mother solution, fluridone mother solution, cyproconazole mother solution and surfactant; The naphthylacetic acid mother solution is prepared by adding 5 mL of 70% anhydrous ethanol to 1 g of naphthylacetic acid, and then adding water to make the volume to 1 L after the naphthylacetic acid is fully dissolved; The cytokinin mother solution is prepared by adding 5 mL of 1 mol / L sodium hydroxide solution to 1 g of cytokinin, and then adding water to make the volume to 1 L after the solution is fully dissolved. The fluoropyridone mother solution is prepared by adding 5 mL of DMSO to 1 g of fluoropyridone to fully dissolve it and then adding 995 mL of water; The cyproconazole mother solution is prepared by adding 1 L of water to 1 g of cyproconazole; The volume ratio of the naphthylacetic acid mother solution, the cytokinin mother solution, the fluridone mother solution, the cyprodinil mother solution and the water is (93-931): (113-1126): 82: 5: 50000; The volume ratio of surfactant to water is 1:10000.

2. The method for preparing the plant growth regulator for preventing the occurrence of cut rose flower necrosis according to claim 1, characterized in that: The steps include: Step 1: respectively prepare naphthylacetic acid mother solution, cytokinin mother solution, fluridone mother solution, and cyproconazole mother solution; Step 2: Add the naphthylacetic acid mother solution, cytokinin mother solution, fluridone mother solution, and cyprodinil mother solution into water, stir evenly, and obtain a mixed solution; Step 3: Add a surfactant to the mixed solution, shake and mix thoroughly, and then adjust the pH of the mixed solution to 5.8-6.0, to finally obtain the plant growth regulator for preventing the occurrence of cut rose flower necrosis.

3. Use of the plant growth regulator for preventing the occurrence of cut rose necrosis according to claim 1 in preventing the occurrence of cut rose necrosis.

4. The use according to claim 3, characterized in that: include: A plant growth regulator composition for preventing the occurrence of cut rose flower buds is sprayed on the flower buds of cut rose plants that are about to complete flower bud differentiation, wherein the diameter of the flower buds is 0.8-1.2 cm; The spraying time is from 16:00 to 18:00 on a sunny afternoon. Keep the plants dry during spraying and avoid spraying at noon.

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