Indoxacarb nano red fire ant poison bait and its preparation method and application

Abstract

The invention discloses an indoxacarb nano red fire ant poison bait and a preparation method and application thereof, belonging to the technical field of red fire ant control. The indoxacarb nano red fire ant poison bait is composed of the following raw materials in mass percentage: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% adhesive, 1.32% to 2.15% nano carrier, 0.09% to 0.15% fatty acid, and 67.65% to 68.54% filler. The indoxacarb nano red fire ant poison bait provided by the invention has a good control effect on red fire ants, and has the advantages of fast effect, strong lasting effect, thorough control, and small amount of application, and the ingredients are stable, not easy to volatilize, safe and non-toxic, and the damage to the surrounding environment and animals and plants of the application is small, which meets the characteristics of green pesticides; the preparation method of the indoxacarb nano red fire ant poison bait provided is simple and easy to operate, does not require high temperature and high pressure, has low requirements on equipment, does not generate toxic and harmful substances, and is green and environmentally friendly.

Description

Technical Field

[0001] The invention relates to the technical field of red fire ant prevention and control, in particular to an indoxacarb nano red fire ant poison bait and a preparation method and application thereof. Background Art

[0002] The red fire ant (Solenopsis invicta Buren) is one of the 100 most threatening invasive alien species in the world. As of July 2021, the red fire ant has spread to nearly 500 counties and districts in 12 provinces in my country. The red fire ant has strong reproductive capacity and spreads quickly. It is easy for it to become the dominant population in the invaded area, which not only destroys the original ecological balance but also causes extremely serious harm to humans and livestock. It is of great significance to carry out the prevention and control of the red fire ant.

[0003] In the prevention and control of red fire ants, the main methods used at home and abroad are to treat individual ant nests with chemical agents and spread poison baits. The current methods have obvious effects on the prevention and control of red fire ants in the short term, but the long-term effect is very poor and the prevention and control is not thorough. Many regions have reported that red fire ants will become rampant again in the affected areas one year after prevention and control.

[0004] Indoxacarb is a recommended agent in my country's "Technical Regulations for Chemical Control of Red Fire Ants". Reports show that indoxacarb is effective quickly, but has a short duration. Nanopesticides have the advantages of controlling the release rate of pesticides, extending the duration of effectiveness, reducing the amount of pesticides released in non-target areas and environments, and reducing residual pollution. They are currently a hot topic in the field of nanotechnology application research in agriculture, but there are no reports on nano-preparations of indoxacarb for the control of red fire ants.

[0005] Deep Eutectic Solvent (DES) is a new molecular complex formed by hydrogen bonding of two or three compounds. It not only has similar physical and chemical properties and corresponding advantages as ionic liquids, but also has stable composition, is not easy to volatilize, is safe and non-toxic, and is easy to store. However, there is no report on the preparation of indoxacarb as a eutectic solvent. Summary of the invention

[0006] The purpose of the present invention is to provide an indoxacarb nano red fire ant poison bait and a preparation method and application thereof, so as to solve the problems of large dosage of red fire ant control, poor persistence, incomplete control, and great potential safety hazards to the ecological environment around the application of the pesticide and the life and health of personnel.

[0007] To achieve the above-mentioned purpose, the present invention provides an indoxacarb nano red fire ant poison bait and a preparation method and application thereof. The indoxacarb nano red fire ant poison bait is composed of the following raw materials in percentage by mass: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% adhesive, 1.32% to 2.15% nano carrier, 0.09% to 0.15% fatty acid, and 67.65% to 68.54% filler.

[0008] Preferably, the composition is composed of the following raw materials in percentage by mass: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 1.38% nanocarrier, 0.12% fatty acid, and 68.45% filler.

[0009] Preferably, the composition is composed of the following raw materials in percentage by mass: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 1.32% nanocarrier, 0.09% fatty acid, and 68.54% filler.

[0010] Preferably, the composition is composed of the following raw materials in percentage by mass: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 2.15% nanocarrier, 0.15% fatty acid, and 67.65% filler.

[0011] Preferably, the sweetener is one or more of white sugar, brown sugar, and maltose; the binder is one or more of peanut oil, soybean oil, rapeseed oil, sunflower oil, and sesame oil; the attractant is a mixture of chicken meal and fish meal, and the mass ratio of chicken meal to fish meal is 1:1; the nanocarrier is calcium carbonate; the fatty acid is one of oleic acid, linoleic acid, and linolenic acid; and the filler is one or more of corn flour, wheat flour, and potato starch.

[0012] A method for preparing the above-mentioned indoxacarb nano red fire ant poison bait comprises the following steps:

[0013] S1. Accurately weighing each component in proportion, mixing indoxacarb with fatty acid to prepare a eutectic solvent;

[0014] S2, coating the eutectic solvent prepared in step S1 with a nanocarrier to obtain a coated indoxacarb nanomaterial;

[0015] S3, adding a sweetener, an attractant, a binder, and a filler to the coated indoxacarb nano material prepared in step S2, stirring evenly, and then crushing through a 14-mesh sieve to obtain the indoxacarb nano red fire ant bait.

[0016] Preferably, the preparation method of the co-melting solvent in step S1 is: after indoxacarb and fatty acid are uniformly mixed in proportion, heating is carried out under vacuum at 80° C. for 2 h, the temperature is adjusted to 100° C., and heating is continued for 2 h.

[0017] Preferably, the method for obtaining the coated indoxacarb nanomaterial in step S2 is: adding FMES to the indoxacarb eutectic solvent, mixing evenly and adding to a calcium chloride solution, dropping a sodium carbonate solution under stirring conditions at 1000 rpm, continuing to stir for 1 hour after the dropwise addition is completed, then standing for 12 hours, centrifuging at a speed of 10000 rpm for 10-12 minutes, washing with distilled water 3 times, and vacuum drying overnight.

[0018] Preferably, the volume ratio of the indoxacarb eutectic solvent:FMES is 0.1:1, and the vacuum drying temperature is 70°C.

[0019] Application of indoxacarb nano red fire ant poison bait prepared by the preparation method described above in the prevention and treatment of red fire ants.

[0020] Therefore, the indoxacarb nano red fire ant poison bait and its preparation method and application provided by the present invention have the following specific technical effects:

[0021] (1) The indoxacarb nano red fire ant poison bait provided by the present invention has a good control effect on red fire ants, and has the advantages of rapid effect, strong lasting effect, thorough control, and low application amount;

[0022] (2) The indoxacarb nano red fire ant bait prepared by the preparation method provided by the present invention has stable ingredients, is not easy to volatilize, is safe and non-toxic, has little harm to the surrounding environment and animals and plants during application, and meets the characteristics of green pesticides;

[0023] (3) The preparation method of the indoxacarb nano red fire ant bait provided by the present invention is simple and easy to operate, does not require high temperature and high pressure, has low requirements on equipment, does not generate any toxic or harmful substances, and is green and environmentally friendly.

[0024] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0026] Figure 1 The SEM photographs and size statistics of the coated indoxacarb nanomaterial prepared in Example 1, wherein Part A and Part B are SEM photographs of different field sizes, the scale in Part A is 300 nm, the scale in Part B is 10 μm, and Part C is the size statistics of the coated indoxacarb nanomaterial;

[0027] Figure 2 It is the SEM photo of the coated indoxacarb nanomaterial prepared in Example 2 and the size statistics, wherein Part A and Part B are SEM photos of different field sizes, the scale in Part A is 1 μm, the scale in Part B is 3 μm, and Part C is the size statistics of the coated indoxacarb nanomaterial;

[0028] Figure 3 It is the SEM photo and size statistics of the coated indoxacarb nanomaterial prepared in Example 3, wherein Part A and Part B are SEM photos of different field sizes, the scale in Part A is 1 μm, the scale in Part B is 5 μm, and Part C is the size statistics of the coated indoxacarb nanomaterial. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0030] In order to make the purpose, technical scheme and advantages of the present application clearer, more thorough and more complete, the technical scheme of the present invention is clearly and completely described below through the accompanying drawings and examples. The following detailed descriptions are all descriptions of the embodiments, and are intended to provide further detailed descriptions of the present invention. Unless otherwise specified, all technical terms used in the present invention have the same meaning as those generally understood by those skilled in the art to which the present application belongs.

[0031] The instruments, equipment, reagents and materials used in the examples were all obtained through commercial channels.

[0032] Embodiment 1

[0033] A method for preparing an indoxacarb nano red fire ant poison bait is as follows:

[0034] S1. Accurately weigh 5.498 g of indoxacarb and 2.825 g of oleic acid, stir and mix, put the mixture into a vacuum drying oven, heat it at 80° C. under vacuum for 2 h, then adjust the temperature to 100° C. and continue heating for 2 h to obtain an indoxacarb eutectic solvent.

[0035] S2. 100 μL of the indoxacarb eutectic solvent prepared in step S1 was taken, 1 mL of FMES was added thereto, stirred and mixed, and then dissolved in 50 mL of 0.04 M calcium chloride solution (containing 10% ethylene glycol), and 50 mL of 0.04 M sodium carbonate solution (containing 10% ethylene glycol) was added dropwise under stirring at 1000 rpm. After the addition was completed, stirring was continued for 1 h. After standing for 12 h, the mixture was centrifuged at 10000 rpm for 12 min. The precipitate was washed 3 times with distilled water, and completely dried in a vacuum drying oven at 70° C. to obtain the coated indoxacarb nanomaterial.

[0036] S3. Weigh 3.10 g of the coated indoxacarb nanomaterial prepared in step S2, add 20 g of white sugar, 20 g of a mixture of chicken meal and fish meal (mass ratio of 1:1), 20 g of peanut oil, and 136.90 g of corn flour, stir evenly, grind with a mortar, and pass through a 14-mesh sieve to obtain the indoxacarb nano red fire ant bait, which is stored at 4 ° C for later use.

[0037] Results Analysis

[0038] 1. The drug loading and coverage rate of the coated indoxacarb nanomaterial obtained in step S2 are investigated as follows:

[0039] 5, 10, 15, 20, and 25 μg / mL indoxacarb ethanol solutions were prepared, and the absorbance of the solutions was measured using a UV spectrophotometer. A standard indoxacarb curve was plotted with concentration as the abscissa and absorbance as the ordinate, and the linear equation of the standard curve was calculated to be y=0.0271x+0.1128.

[0040] 8 mg of the coated indoxacarb nanomaterial was dispersed in 8 mL of anhydrous ethanol, and the indoxacarb coated therein was released by ultrasonication at 500 W for 20 min. The absorbance of the prepared nanomaterial was tested by an ultraviolet spectrophotometer, and the indoxacarb content of the nanomaterial was calculated by the indoxacarb standard curve. It was calculated that the coated indoxacarb nanomaterial used in step S3 contained 0.1 g of indoxacarb and 0.24 g of oleic acid, and the drug loading and coating rate of the nanomaterial were calculated according to the following formula:

[0041]

[0042]

[0043] The results showed that after being coated with calcium carbonate, the drug loading of the eutectic solvent of oleic acid and indoxacarb was 3.23% and the coating rate was 12.19%.

[0044] 2. The apparent morphology, hydrated particle size, zeta potential and polydispersity index (PDI) of the coated indoxacarb nanomaterial prepared in step S2 were investigated as follows:

[0045] Weigh 10 mg of the coated indoxacarb nanomaterial prepared in step S2, add 10 mL of anhydrous ethanol, and ultrasonically disperse for 30 min. Draw 10 μL of the dispersed liquid droplets on the surface of the single crystal silicon wafer and air dry naturally. After gold spraying, observe using a field emission scanning electron microscope S-4800 (SEM). The results are shown in Figure 1 The diameters of 200 nanoparticles were randomly selected and measured using Image J (V13.0.6.). The particle size frequency distribution histogram was drawn using Graphpadprism, and the average particle size was calculated. Figure 1 Part C of

[0046] Weigh 10 mg of material, add 10 mL of anhydrous ethanol, and ultrasonically disperse for 30 minutes. Use a nanoparticle size and zeta positioner to determine the hydrated particle size, surface Zeta potential and polydispersity index (PDI) of the material in aqueous solution. Repeat the measurement three times for each treatment.

[0047] Depend on Figure 1 As can be seen from the A and B parts of FIG. 2 , the coated indoxacarb nanomaterial prepared in step S2 is substantially round, uniform in size, and has a rough surface, and is formed into a single-shell microcapsule by calcium carbonate deposition on the surface of the eutectic solvent droplet. Figure 1 It can be seen from the C part that CaCO 3 The average particle size of the coated oleic acid eutectic solvent is 0.591±0.102μm. The hydrated particle size of the nanomaterial is 753.6±18.809nm, the zeta potential is 29.5±1.155mV, and the PDI is 0.3±0.011 as measured by the nanoparticle size and zeta positioning instrument.

[0048] Embodiment 2

[0049] A method for preparing an indoxacarb nano red fire ant poison bait is as follows:

[0050] S1. Accurately weigh 5.498 g of indoxacarb and 2.984 g of linoleic acid, stir and mix, put the mixture into a vacuum drying oven, heat it at 80° C. under vacuum for 2 h, then adjust the temperature to 100° C. and continue heating for 2 h to obtain an indoxacarb eutectic solvent.

[0051] S2. Prepare coated indoxacarb nanomaterials according to the method of S2 in Example 1.

[0052] S3. Weigh 2.92 g of the coated indoxacarb nanomaterial prepared in step S2, add 20 g of white sugar, 20 g of a mixture of chicken meal and fish meal (mass ratio of 1:1), 20 g of peanut oil, and 137.08 g of corn flour, stir evenly, grind with a mortar, and pass through a 14-mesh sieve to obtain the indoxacarb nano red fire ant bait, which is stored at 4 ° C for later use.

[0053] Results Analysis

[0054] The method for investigating the prepared coated indoxacarb nanomaterial is exactly the same as that in Example 1. It is calculated that the coated indoxacarb nanomaterial used in step S3 contains 0.1g indoxacarb and 0.18g linoleic acid. The result is that the drug loading of the eutectic solvent of linoleic acid and indoxacarb is 3.42%, and the coverage rate is 13.00%.

[0055] Scanning electron microscopy revealed that the coated indoxacarb nanomaterials were basically round, uniform in size, and had a rough surface. They were formed into single-shell microcapsules by calcium carbonate deposition on the surface of the eutectic solvent droplets (see Figure 2 Part A and Part B), CaCO 3 The average particle size of the coated oleic acid eutectic solvent is 0.731±0.120μm (see Figure 2 Part C). The hydrated particle size of the nanomaterial was determined to be 929.5±14.812nm, the zeta potential was 29.0±1.19mV, and the PDI was 0.092±0.04.

[0056] Embodiment 3

[0057] A method for preparing an indoxacarb nano red fire ant poison bait is as follows:

[0058] S1. Accurately weigh 5.498 g of indoxacarb and 3.480 g of linolenic acid, stir and mix, put the mixture into a vacuum drying oven, heat it at 80° C. under vacuum for 2 h, then adjust the temperature to 100° C. and continue heating for 2 h to obtain an indoxacarb eutectic solvent.

[0059] S2. Prepare coated indoxacarb nanomaterials according to the method of S2 in Example 1.

[0060] S3. Weigh 4.7 g of the coated indoxacarb nanomaterial prepared in step S2, add 20 g of white sugar, 20 g of a mixture of chicken meal and fish meal (mass ratio of 1:1), 20 g of peanut oil, and 135.30 g of corn flour, stir evenly, grind with a mortar, and pass through a 14-mesh sieve to obtain the indoxacarb nano red fire ant bait, which is stored at 4 ° C for later use.

[0061] Results Analysis

[0062] The method for examining the prepared coated indoxacarb nanomaterial is exactly the same as that in Example 1. It is calculated that the coated indoxacarb nanomaterial used in step S3 contains 0.1g indoxacarb and 0.30g linolenic acid. The result is that the drug loading of the eutectic solvent of linolenic acid and indoxacarb is 2.13%, and the coverage rate is 9.27%.

[0063] Scanning electron microscopy revealed that the coated indoxacarb nanomaterials were also basically round, uniform in size, and rough on the surface. They were formed into single-shell microcapsules by calcium carbonate deposition on the surface of the eutectic solvent droplets (see Figure 3 Part A and Part B), CaCO 3 The average particle size of the linolenic acid eutectic solvent is 0.901±0.145μm (see Figure 3 Part C). The hydrated particle size of the nanomaterial was determined to be 1504±4.242nm, the zeta potential was 41.0±2.313mV, and the PDI was 0.236±0.016.

[0064] Effect example

[0065] The control effect of the indoxacarb nano red fire ant bait prepared in the example on red fire ants was investigated as follows:

[0066] Select the occurrence areas of red fire ants with a severity of level 4 or above to divide into small areas, with an area of ​​100m 2 There are at least 10 active ant nests in each community. The base number of worker ants in the ant nests was investigated using the ham sausage trapping method. The trapping method refers to the "GB / T 23626-2009 Red Fire Ant Epidemic Monitoring Procedure". The specific method is as follows:

[0067] Place the ham bait in a 50mL centrifuge tube and mark the treatment to make a monitoring bottle. At around 9:00 am, insert the experimental treatment sign next to the determined test ant nest and place the monitoring bottle 50cm away from the ant nest. Set up 3 monitoring points for each treatment, and place 4 monitoring bottles at each monitoring point in the 4 directions of east, south, west and north. After 30 minutes, cover, seal and retract the monitoring bottle, add 5mL of 75% ethanol, and record the number of traps in each bottle after the red fire ants die. If other types of ants are trapped, they should be properly preserved and identified. In subsequent investigations and traps, the monitoring bottles will be placed at the location of the first investigation.

[0068] The baits prepared in Examples 1 to 3 were respectively recorded as treatment A, treatment B, and treatment C. 20 g of each was taken and spread around the ant nests, and a commercial agent (0.05% indoxacarb ant killing bait) was used as the agent control group, and an ant nest without any treatment was used as a blank control. Each treatment was repeated 3 times. The control effect of worker ants was counted by trapping method on the 7th, 14th, and 21st day after the application of the agent, and the ant nests were excavated after the last trapping to count the live ant nests, ant colonies, and comprehensive control effects.

[0069] (1) Effectiveness of controlling live ant nests

[0070] According to the survey results, the control effect of live ant nests is calculated according to the following formula:

[0071]

[0072] Where: PN is the control effect of live ant nests; N 0 N is the number of live ant nests in the control area before treatment; Ti N is the number of live ant nests in the treated area after drug administration; 0i N is the number of live ant nests in the control area after drug administration; T0 It is the number of live ant nests in the treatment area before drug treatment.

[0073] (2) Effect of worker ant control

[0074] According to the survey results of the trapping method, the control effect of worker ants is calculated according to the following formula:

[0075]

[0076] Where: PW is the control effect of worker ants; W 0 W is the average number of worker ants in the monitoring bottle of the control area before drug administration; Ti W is the average number of worker ants in the monitoring bottles in the post-drug treatment area; 0i W is the average number of worker ants in the monitoring bottle of the control area after drug administration; T0 The average number of worker ants in the monitoring bottles for the pre-drug treatment area.

[0077] (3) Ant colony control effect

[0078] Based on the results of the nest excavation inspection, the survival of the red fire ant colonies in the ant nests was graded according to the ant colony classification standards, and the average level of all ant colonies in each community was calculated. Based on the results of the ant colony level survey, the ant colony level reduction rate was calculated.

[0079] Ant colony classification standards:

[0080] Level 0: No insect stage survives;

[0081] Level 1: Only worker ants or pupae and larvae survive;

[0082] Level 2: There are winged reproductive ants and other insect-like individuals except the queen ant alive;

[0083] Level 3: The queen ant and other insect-like individuals survive.

[0084] Ant colony control effect calculation formula:

[0085]

[0086] Where: PC is the ant colony control effect; C 0 is the average level of ant colonies in the control area before drug administration; C Ti is the average level of ant colonies in the post-drug treatment area; C 0i is the average level of ant colonies in the control area after drug administration; C T0 It is the average level of ant colonies in the pre-drug treatment area.

[0087] (4) Comprehensive control effect

[0088] The weight coefficients of the control effect of live ant nests, worker ants and ant colonies are assigned as 0.3, 0.2 and 0.5 respectively, and the calculation of the comprehensive control effect is as follows:

[0089] P(%)=0.3PN+0.2PW+0.5PC

[0090] Where: P is the comprehensive control effect; PN is the control effect of live ant nests; PW is the control effect of worker ants; PC is the control effect of ant colonies.

[0091] Prevention and treatment results

[0092] 1. The control results of worker ants are shown in Table 1. After 7 days of application, the control effect of the indoxacarb nano preparations prepared in Examples 1 to 3 exceeded 50%, but was lower than the control group; after 14 days of application, the control effect of treatment C was significantly higher than the control group, and the control effect of treatment A was equivalent to that of the commercial agent; after 21 days of application, the control effect of treatment C could reach 100%, followed by the control effect of treatment A, which could also reach more than 80%, and the control effect of treatment B was higher than the control group, but there was no significant difference. Compared with the three nano baits, treatment C had the most ideal effect and good lasting effect.

[0093] Table 1 Control effect of indoxacarb nano bait on worker ants (%)

[0094]

[0095] 2. The results of the control effect on live ant nests are shown in Table 2. Among the control effects on live ant nests, all the agents had no control effect on the ant nests after 7d and 14d of application. After 21d of application, the control rate of the ant nests by the C treatment can reach 100%, which is significantly different from other treatments. Secondly, the control rate of the ant nests by the A treatment is close to 80%, and the effect of the B treatment is equivalent to that of the commercial agent, with no significant difference. Among the three nano baits prepared in Examples 1 to 3, the nano bait prepared in Example 3 (C treatment) has the most ideal effect.

[0096] Table 2 The control effect of indoxacarb nano bait on ant nests (%)

[0097]

[0098] 3. Effect on ant colony control

[0099] The results are shown in Table 3. Among the control effects on ant colonies, only treatment C had a control effect of 100% 21 days after application, which was significantly different from the other treatments; treatment A also had a control effect of more than 60% on ant colonies, which was significantly different from the control; treatment B had an effect comparable to the commercial agent, with no significant difference. Among the three nano baits, treatment C had the most ideal effect.

[0100] Table 3 Control effect of indoxacarb nano bait on ant colonies (%)

[0101] deal with Pre-drug ant colony level Ant colony level after medication Effectiveness% A 3 1 <![CDATA[66.7 b ]]> B 3 2 <![CDATA[33.3 c ]]> C 3 0 <![CDATA[100 a ]]> Drug control 3 2 33.3c Blank control 3 3 -

[0102] 4. Comprehensive prevention and control effect

[0103] The results are shown in Table 4. After 21 days of application, the comprehensive control effect of treatment C on red fire ants can reach 100%, and the comprehensive control effect of treatment A can reach more than 70%. Treatment B is equivalent to the commercial agent, with no significant difference. Among the three nano baits, treatment C is still the most ideal.

[0104] Table 4 Comprehensive control effect of indoxacarb nano bait on red fire ants (%)

[0105]

[0106]

[0107] Therefore, the indoxacarb nano red fire ant poison bait provided by the present invention has a good control effect on red fire ants, and has the advantages of quick effect, strong lasting effect, thorough control, and small application amount, and has stable ingredients, is not easy to volatilize, is safe and non-toxic, has little harm to the surrounding environment and animals and plants when applying the pesticide, and has the characteristics of a composite green pesticide; the preparation method of the indoxacarb nano red fire ant poison bait provided is simple and easy to operate, does not require high temperature and high pressure, has low requirements on equipment, does not generate toxic and harmful substances, and is green and environmentally friendly.

[0108] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A nano red imported fire ant bait containing indoxacarb, characterized in that: The invention is composed of the following raw materials in percentage by mass: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 1.32% to 2.15% nanocarrier, 0.09% to 0.15% fatty acid, and 67.65% to 68.54% filler; The fatty acid is one of oleic acid, linoleic acid, and linolenic acid; The preparation method of the indoxacarb nano red fire ant poison bait is characterized by the following steps: S1. Accurately weighing each component in proportion, mixing indoxacarb with fatty acid to prepare a eutectic solvent; S2, coating the eutectic solvent prepared in step S1 with a nanocarrier to obtain a coated indoxacarb nanomaterial; S3, adding a sweetener, an attractant, a binder, and a filler to the coated indoxacarb nano material prepared in step S2, stirring evenly, and then crushing through a 14-mesh sieve to obtain an indoxacarb nano red fire ant bait; The preparation method of the co-melting solvent in step S1 is: indoxacarb and fatty acid are uniformly mixed in proportion, heated under vacuum at 80° C. for 2 h, then the temperature is adjusted to 100° C., and the heating is continued for 2 h; The method for obtaining the coated indoxacarb nanomaterial in step S2 is as follows: adding FMES to the indoxacarb eutectic solvent, mixing well and then adding to a calcium chloride solution containing 10% ethylene glycol, dripping a sodium carbonate solution containing 10% ethylene glycol under a stirring condition of 1000 rpm, stirring for 1 hour after the dropwise addition is completed, then standing for 12 hours, centrifuging at a speed of 10000 rpm for 10-12 minutes, washing with distilled water 3 times, and vacuum drying overnight; The volume ratio of the indoxacarb eutectic solvent to FMES is 0.1:1, and the vacuum drying temperature is 70°C.

2. The indoxacarb nano red fire ant poison bait according to claim 1, characterized in that: The invention is composed of the following raw materials in the following mass percentages: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 1.38% nanocarrier, 0.12% fatty acid and 68.45% filler.

3. The indoxacarb nano red fire ant poison bait according to claim 1, characterized in that: The invention is composed of the following raw materials in the following mass percentages: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% binder, 1.32% nanocarrier, 0.09% fatty acid and 68.54% filler.

4. The indoxacarb nano red fire ant poison bait according to claim 1, characterized in that: The invention is composed of the following raw materials in the following mass percentages: 0.05% indoxacarb, 10% sweetener, 10% attractant, 10% adhesive, 2.15% nanocarrier, 0.15% fatty acid and 67.65% filler.

5. The indoxacarb nano red fire ant poison bait according to claim 1, characterized in that: The sweetener is one or more of white sugar, brown sugar, and maltose; the adhesive is one or more of peanut oil, soybean oil, rapeseed oil, sunflower oil, and sesame oil; the attractant is a mixture of chicken meal and fish meal, and the mass ratio of chicken meal to fish meal is 1:1; the nanocarrier is calcium carbonate; and the filler is one or more of corn flour, wheat flour, and potato starch.

6. Use of the indoxacarb nano red fire ant bait as claimed in any one of claims 1 to 5 in the control of red fire ants.

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