A kind of anti-hair loss composition based on plant extract and preparation method thereof

Abstract

The present invention discloses a plant extract-based anti-hair loss composition and its preparation method. The plant extract-based anti-hair loss composition comprises a plant extract and an active peptide; the plant extract is at least one of wheat germ extract, coralline algae extract, and Gynostemma pentaphyllum extract. The present invention also provides a preparation method. Compared with the prior art, the present invention utilizes a rational combination of wheat germ extract, coralline algae extract, and Gynostemma pentaphyllum extract with the active peptide, resulting in a complementary and synergistic anti-hair loss mechanism. The composition of the present invention can provide essential nutrients for all stages of hair growth, effectively repair scalp follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, and effectively improve the scalp environment. It also has a rapid and mild effect.

Description

Technical Field

[0001] The present invention relates to the technical field of daily necessities, and in particular to a plant extract-based anti-hair loss composition and a preparation method thereof. Background Art

[0002] Alopecia is the loss of hair, which can be either physiological or pathological. Alopecia is usually caused by an imbalance in the hair follicle's growth cycle. Normally, hair growth cycles through the growth phase, the catagen phase, and the resting phase. During the resting phase, hair is prone to falling out, and shedding 50 to 100 hairs per day is a normal physiological phenomenon. However, pathological alopecia involves daily hair loss in greater quantities and a lack of regeneration, resulting in thinning hair or baldness.

[0003] Chronic stress and tension are thought to be linked to hair loss. Research published in a related academic journal has found that the adrenal stress hormone corticosterone plays a key role in the quiescence and hair growth of hair follicle stem cells (HFSCs). This research reveals the underlying mechanism by which chronic stress leads to hair loss, providing new research directions for treating hair loss.

[0004] In summary, the research progress of hair loss involves many aspects, from basic physiological mechanisms to chronic stress, androgenic alopecia and the role of the immune system.

[0005] CN108310067A discloses an anti-hair loss Chinese herbal composition, a preparation method, and its use in the preparation of anti-hair loss daily chemical products. The anti-hair loss Chinese herbal composition comprises the following raw materials in parts by weight: 10-50 parts of Platycladus orientalis leaves, 5-12 parts of Scutellaria baicalensis, 1-10 parts of Morus alba bark, 1-3 parts of Eclipta prostrata, and 4-7 parts of Plantago seeds. The anti-hair loss Chinese herbal composition of the present invention uses a natural medicinal plant formula and incorporates five active ingredients from traditional Chinese medicine essences, fully exerting the efficacy of Chinese herbal medicines. It can be rapidly absorbed through the skin by hair follicles, making the shampoo non-irritating and non-damaging to the hair, and is quickly absorbed deep into the hair follicles, promoting blood circulation in the scalp and maintaining the vitality of the hair follicles. It has the advantages of growing hair and continuously improving hair loss. However, the effect needs to be further improved. Summary of the Invention

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is an anti-hair loss composition based on plant extracts, which adopts a reasonable combination of plant extracts and modified active peptides so that they complement each other in the anti-hair loss mechanism and play a synergistic role. The composition of the present invention can provide necessary nutrients for all stages of hair growth, effectively repair scalp follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, effectively improve the scalp environment, and has a fast effect and mild effect. At the same time, the active peptide maintains its original performance and improves its storage performance.

[0007] To achieve the above object, the present invention provides an anti-hair loss composition based on plant extracts, characterized in that it consists of active peptides and plant extracts; wherein the mass ratio of the active peptides to the plant extracts is (1-3): (1-3).

[0008] Wherein, the plant extract is at least one of wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract.

[0009] The preparation method of the active peptide comprises the following steps: shelling and cleaning marine shellfish, adding 2-3 times the mass of water, homogenizing for 1-10 minutes, then boiling for 1-5 minutes, cooling to room temperature, adding enzyme preparation and enzymatic hydrolysis for 30-60 minutes, finally inactivating the enzyme, centrifuging to obtain the supernatant, ultrafiltration through a 5000Da ultrafiltration membrane, and freeze-drying to obtain the active peptide.

[0010] The marine shellfish is at least one of oysters, paphia undulata, clams, scallops and pearl oysters.

[0011] The amount of the enzyme preparation is 0.01-0.5wt% of the oyster mass;

[0012] The enzyme preparation is at least one of bromelain, neutral protease and pepsin.

[0013] Wheat germ extract contains unsaturated fatty acids, vitamins, and various minerals. It not only provides rich nutrients for hair growth and effectively maintains scalp elasticity, but also has antibacterial, repairing, and anti-hair loss effects on the hair and scalp.

[0014] Coralline algae extract is rich in marine enzymes, collagen, and minerals, including trace elements such as magnesium, zinc, manganese, and calcium. It replenishes trace elements for hair and can also act as a cell activator and bioreaction catalyst to solve the problem of hair loss caused by mineral deficiency.

[0015] Gynostemma pentaphyllum (Gynostemma pentaphyllum Mark) is a perennial vine in the Cucurbitaceae family, primarily found in the Yangtze River basin and southern Shaanxi. Rich in ginsenosides, which resemble ginsenosides, and richer in amino acids than ginseng, it is known as the "Southern Ginseng." It possesses both nutritional and therapeutic properties. Gynostemma pentaphyllum saponins can penetrate hair fibers and be absorbed by the hair. This is due to the multi-hydroxyl structure of the saponins forming hydrogen bonds with the multi-fatty groups in the hair fibers, connecting them. This increases the hair's strength and elongation, thereby increasing its toughness and resilience, making it less susceptible to breakage and loss. It also leaves hair translucent and shiny, while also removing grease and cleansing hair follicles, thereby improving hair loss.

[0016] Marine shellfish are rich in physiologically active substances such as proteins, peptides, amino acids, polysaccharides and trace elements; among them, bioactive peptides refer to peptide compounds that are beneficial to the life activities of biological organisms or have physiological effects. They are small molecule substances that can drive amino acids into hair follicles, stimulate scalp blood vessel proliferation, accelerate hair metabolism, and can effectively help hair growth and reduce hair loss.

[0017] The present invention adopts a rational combination of plant extracts and active peptides, so that they complement each other in the anti-hair loss mechanism and play a synergistic role. The composition of the present invention can provide necessary nutrients for each stage of hair growth, effectively repair scalp follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, and effectively improve the scalp environment. It has a fast effect and a mild effect.

[0018] However, marine shellfish active peptides are easily inactivated by environmental factors such as temperature, oxygen, and humidity, affecting their stability and sustained activity.

[0019] Further preferably, an anti-hair loss composition based on plant extracts is composed of a modified active peptide and a plant extract; wherein the mass ratio of the modified active peptide to the plant extract is (1-3): (1-3);

[0020] The preparation method of the modified active peptide comprises the following steps:

[0021] (1) Preparation of active peptides: shell the marine shellfish, clean it, add 2-3 times the weight of water, homogenize it for 1-10 minutes, then boil it for 1-5 minutes, cool it to room temperature, add enzyme preparation and hydrolyze it for 30-60 minutes, inactivate the enzyme, centrifuge and take the supernatant, pass the supernatant through a 5000Da ultrafiltration membrane for ultrafiltration, and freeze-dry to obtain the active peptides;

[0022] (2) Add 10-20 parts by weight of porous starch to 100-120 parts by weight of water and stir evenly, heat to 30-40°C, add 1-5wt% of aqueous hydroxide solution to adjust the pH to 7.8-8.2, continue to maintain for 10-20 minutes, then add 3-8 parts by weight of succinic anhydride and stir evenly, heat to 30-40°C and react for 30-60 minutes, filter, wash and dry after the reaction to obtain modified porous starch; add 5-10 parts by weight of modified porous starch to 50-100 parts by weight of water and mix evenly, then add 1-7 parts by weight of active peptide, shake and adsorb at 30-35°C for 30-60 minutes; then add 2-7 parts by weight of carboxylated bacterial cellulose, 3-10 parts by weight of tripropylene glycol, 1-5 parts by weight of silk fibroin, and 0.1-0.6 parts by weight of dibutyltin dilaurate, heat to 50-60°C and react for 30-60 minutes, spray dry after the reaction to obtain modified active peptide.

[0023] The present invention uses succinic anhydride to modify porous starch to enhance its adsorption capacity for active peptides; the porous starch containing active groups, carboxylated bacterial cellulose, and trimeryl glycol undergo an esterification reaction under the action of a catalyst, and simultaneously interact with the active groups in silk fibroin and porous starch to form a good coating layer for the active peptides; thereby alleviating the problem that the marine shellfish active peptides are easily inactivated by environmental factors such as temperature, oxygen, and humidity, thereby maintaining their original properties and improving their storage performance.

[0024] The invention also discloses application of the anti-hair loss composition based on plant extracts in hair care products.

[0025] In certain embodiments, the hair care product comprises at least one of shampoo, essence, conditioner, hair spray, and hair mask, but is not limited to shampoo, essence, conditioner, hair spray, and hair mask. The present invention also provides a shampoo comprising an anti-hair loss composition based on a plant extract.

[0026] A shampoo comprising an anti-hair loss composition based on plant extracts, comprising:

[0027] 5-15wt% of the above-mentioned anti-hair loss composition based on plant extracts, 1-5wt% of moisturizing agent, 0.1-3wt% of skin conditioning agent, 0.01-1wt% of preservative, 0.01-1wt% of chelating agent, 0.1-4wt% of emulsifier, 0.1-10wt% of surfactant, and the balance is water.

[0028] Wherein, the moisturizing agent is any one of glycerin and propylene glycol;

[0029] Wherein, the skin conditioning agent is any one of niacinamide and panthenol;

[0030] Wherein, the preservative is any one of phenoxyethanol and potassium sorbate;

[0031] Beneficial effects of the present invention:

[0032] 1. Compared with the existing technology, the present invention adopts a rational combination of wheat germ extract, coralline algae extract, Gynostemma pentaphyllum extract and active peptides, so that they complement each other in the anti-hair loss mechanism and play a synergistic role. The composition of the present invention can provide necessary nutrients for each stage of hair growth, effectively repair scalp follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, and effectively improve the scalp environment, with fast effect and mild action.

[0033] 2. Compared with the existing technology, the present invention uses succinic anhydride to modify porous starch to enhance its adsorption capacity for active peptides; porous starch, carboxylated bacterial cellulose, and trimerol containing active groups undergo esterification reaction under the action of a catalyst, and at the same time interact with the active groups in silk fibroin and porous starch to form a good coating layer for the active peptides; thereby improving the problem that marine shellfish active peptides are easily inactivated by environmental factors such as temperature, oxygen, and humidity, so that they maintain their original performance and improve their storage performance. DETAILED DESCRIPTION

[0034] The endpoints of the ranges and any values disclosed herein are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values can be combined with each other to form one or more new numerical ranges, and these numerical ranges should be considered to be specifically disclosed in the present invention.

[0035] Introduction to some of the raw materials used in the embodiments of the present invention:

[0036] The oysters used in the examples were commercially available.

[0037] Bromelain, product number: S10009, was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0038] Neutral protease (product number: S10013) was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0039] The preparation method of the porous starch used in the embodiment is as follows: 2g of cassava starch is added to a 250ml conical flask, 40mL of 0.2mol / L disodium hydrogen phosphate and 0.1mol / L citric acid buffer with a pH of 4.6 is added, 0.1mL of toluene is added, and an enzymatic hydrolyzate is added (the mass of the enzymatic hydrolyzate is 1wt% of the mass of the cassava starch), 40°C, constant temperature oscillation for 24h, centrifugation, and separation of the supernatant. The precipitate is dried at normal pressure at 60°C and crushed. The enzyme in the enzymatic hydrolyzate is a complex enzyme composed of α-amylase (product number: S10003, purchased from Shanghai Yuanye Biotechnology Co., Ltd.) and saccharifying enzyme (product number: S10017, purchased from Shanghai Yuanye Biotechnology Co., Ltd.) in a mass ratio of 1:4.

[0040] The carboxylated bacterial cellulose used in the examples was prepared according to the preparation method in Example 1 of patent CN112587724B; the bacterial cellulose was fermented by Gluconacetobacter xylinus (CGMCC1.1812, purchased from China General Microbial Culture Collection Center) using conventional methods.

[0041] The preparation methods of the wheat germ extract, coralline algae extract, and gynostemma pentaphyllum extract in the embodiments are as follows: wheat germ, coralline algae, and gynostemma pentaphyllum are dried and crushed through a 100-mesh sieve to obtain wheat germ powder, coralline algae powder, and gynostemma pentaphyllum powder, respectively; then, 12 times the weight of a 75wt% ethanol aqueous solution is added, the mixture is soaked at 40°C for 7 hours, low-temperature ultrasonic extraction is performed for 6 hours, the supernatant is centrifuged, filtered, and refluxed with ethanol, and finally, after secondary filtration, freeze-dried to obtain wheat germ extract, coralline algae extract, and gynostemma pentaphyllum extract, respectively.

[0042] All other raw materials not mentioned are commonly used in the art. The above description is merely provided to illustrate the present invention and is not to be construed as a strict limitation of the present invention. Those skilled in the art can purchase or prepare the same or similar raw materials commercially. These details will not be further detailed in the examples.

[0043] The following will be combined with specific embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0044] Example 1

[0045] A plant extract-based anti-hair loss composition consists of active peptides and plant extracts; the mass ratio of the active peptides to the plant extracts is 2:1.

[0046] The plant extract is prepared by mixing wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract in a mass ratio of 2:1:1.

[0047] The active peptide preparation method comprises the following steps: shelling and cleaning oysters, adding 3 times the mass of water, homogenizing for 10 minutes, boiling for 5 minutes, cooling to room temperature, adding an enzyme preparation, and performing enzymatic hydrolysis for 30 minutes. Finally, inactivating the enzyme, centrifuging, obtaining a supernatant, passing the supernatant through a 5000Da ultrafiltration membrane for ultrafiltration, and freeze-drying to obtain the active peptide. The enzyme preparation is used in an amount of 0.5wt% of the mass of the oysters. The enzyme preparation is prepared by mixing bromelain and neutral protease in a mass ratio of 1:1.

[0048] Example 2

[0049] A plant extract-based anti-hair loss composition consists of an active peptide and a plant extract; the mass ratio of the modified active peptide to the plant extract is 1:1.

[0050] The plant extract is prepared by mixing wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract in a mass ratio of 2:1:1.

[0051] The preparation method of the modified active peptide comprises the following steps: (1) shelling and cleaning the oysters, adding 3 times the weight of water, homogenizing for 10 minutes, then boiling for 5 minutes, cooling to room temperature, adding an enzyme preparation and performing enzymatic hydrolysis for 30 minutes, finally inactivating the enzyme, centrifuging and obtaining a supernatant, ultrafiltering the supernatant through a 5000Da ultrafiltration membrane, and freeze-drying to obtain the active peptide; wherein the amount of the enzyme preparation is 0.5wt% of the weight of the oysters; and the enzyme preparation is prepared by mixing bromelain and neutral protease in a mass ratio of 1:1;

[0052] (2) 20 parts by weight of porous starch were added to 120 parts by weight of water and stirred evenly, heated to 35°C, 5 wt% aqueous hydroxide solution was added to adjust the pH to 8, and the pH was maintained for 20 minutes, and then 8 parts by weight of succinic anhydride were added and stirred evenly, heated to 40°C and reacted for 60 minutes. After the reaction was completed, the mixture was filtered, washed, and dried to obtain modified porous starch; 10 parts by weight of modified porous starch were added to 100 parts by weight of water and mixed evenly, and then 7 parts by weight of active peptide were added and oscillated and adsorbed at 30°C for 60 minutes; then 7 parts by weight of carboxylated bacterial cellulose, 10 parts by weight of tripropylene glycol, 5 parts by weight of silk fibroin, and 0.6 parts by weight of dibutyltin dilaurate were added, and the mixture was heated to 55°C and reacted for 30 minutes. After the reaction was completed, the mixture was spray-dried to obtain modified active peptide.

[0053] Example 3

[0054] An anti-hair loss composition based on plant extracts is basically the same as Example 2, except that the mass ratio of the modified active peptide to the plant extract is 2:1.

[0055] Example 4

[0056] An anti-hair loss composition based on plant extracts is basically the same as Example 2, except that the mass ratio of the modified active peptide to the plant extract is 3:1.

[0057] Example 5

[0058] An anti-hair loss composition based on plant extracts is basically the same as Example 2, except that the mass ratio of the modified active peptide to the plant extract is 1:2.

[0059] Example 6

[0060] An anti-hair loss composition based on plant extracts is basically the same as Example 2, except that the mass ratio of the modified active peptide to the plant extract is 1:3.

[0061] Example 7

[0062] A plant extract-based anti-hair loss composition consists of an active peptide and a plant extract; the mass ratio of the modified active peptide to the plant extract is 2:1.

[0063] The plant extract is prepared by mixing wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract in a mass ratio of 2:1:1.

[0064] The preparation method of the modified active peptide comprises the following steps: (1) shelling and cleaning the oysters, adding 3 times the weight of water, homogenizing for 10 minutes, then boiling for 5 minutes, cooling to room temperature, adding an enzyme preparation and performing enzymatic hydrolysis for 30 minutes, finally inactivating the enzyme, centrifuging and obtaining a supernatant, ultrafiltering the supernatant through a 5000Da ultrafiltration membrane, and freeze-drying to obtain the active peptide; wherein the amount of the enzyme preparation is 0.5wt% of the weight of the oysters; and the enzyme preparation is prepared by mixing bromelain and neutral protease in a mass ratio of 1:1;

[0065] (2) 20 parts by weight of porous starch were added to 120 parts by weight of water and stirred evenly, heated to 35°C, 5 wt% aqueous hydroxide solution was added to adjust the pH to 8, and the pH was maintained for 20 minutes, and then 8 parts by weight of succinic anhydride were added and stirred evenly, heated to 40°C and reacted for 60 minutes. After the reaction was completed, the mixture was filtered, washed, and dried to obtain modified porous starch; 10 parts by weight of modified porous starch were added to 100 parts by weight of water and mixed evenly, and then 7 parts by weight of active peptide were added and adsorbed at 30°C under oscillation for 60 minutes; after the reaction was completed, the mixture was spray-dried to obtain modified active peptide.

[0066] Comparative Example 1

[0067] A plant extract-based anti-hair loss composition is substantially the same as that of Example 3, except that the plant extract is wheat germ extract.

[0068] Comparative Example 2

[0069] A plant extract-based anti-hair loss composition is substantially the same as that of Example 3, except that the plant extract is coralline algae extract.

[0070] Comparative Example 3

[0071] A plant extract-based anti-hair loss composition is substantially the same as Example 3, except that the plant extract is a Gynostemma pentaphyllum extract.

[0072] Application Example 1

[0073] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 1, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0074] Application Example 2

[0075] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 2, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0076] Application Example 3

[0077] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 3, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0078] Application Example 4

[0079] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 4, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0080] Application Example 5

[0081] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 5, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0082] Application Example 6

[0083] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 6, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0084] Application Example 7

[0085] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Example 7, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0086] Application Example 8

[0087] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Comparative Example 1, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0088] Application Example 9

[0089] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Comparative Example 2, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0090] Application Example 10

[0091] A shampoo comprising an anti-hair loss composition based on a plant extract, comprising the following substances: 12 wt% of the anti-hair loss composition based on a plant extract of Comparative Example 3, 5 wt% of glycerin, 1 wt% of niacinamide, 0.05 wt% of phenoxyethanol, 0.05 wt% of disodium EDTA, 1 wt% of sucrose fatty acid ester, 3 wt% of sodium laureth sulfate, and the balance being water.

[0092] Test Example 1

[0093] Two hundred volunteers aged between 22 and 50 who suffered from daily hair loss and scalp itching were selected and randomly divided into 20 groups. Each group was treated with the shampoos described in Examples 1-10. Test Method: Each participant shampooed once daily in the morning for one week. Hair loss and scalp itching were observed the following day. The efficacy evaluation criteria are shown in Table 1. The number of participants in each group was summarized in Table 2 based on the efficacy evaluation.

[0094] Table 1 Effect evaluation criteria

[0095] Effect standard Significantly effective No symptoms such as hair loss and scalp itching efficient Symptoms such as hair loss and scalp itching have been significantly improved compared to before the trial invalid Symptoms such as hair loss and scalp itching have not changed before the test

[0096] Table 2 Test results

[0097]

[0098]

[0099] The present invention adopts wheat germ extract, coralline algae extract, and pentaphyllum extract and active peptides extracted from oysters as raw materials and modifies them to work synergistically with each other, providing nutrients to the scalp while effectively maintaining scalp elasticity, and having anti-hair loss and repairing effects on the scalp; on the other hand, it provides the scalp with rich nutrients, removes oil, and cleans hair follicles, thereby improving the problem of hair loss; the composition of the present invention can provide necessary nutrients for all stages of hair growth, effectively repair scalp hair follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, and effectively improve the scalp environment, with fast effect and mild action.

[0100] Test Example 2

[0101] A total of 40 C57BL / 6 female mice (weighing 18-22g) were raised normally for one week, and then hair removal was performed on a 2cm×2cm area on the back of the mice (using a mixture of rosin and paraffin in a weight ratio of 1:1, heated and melted, and then applied to the designated area on the back of the mice). The mice were then randomly divided into groups of 10, one of which served as a blank control group and was only applied with normal saline. The remaining groups were applied with 20mg / kg of the shampoo in each application example, once in the morning and once in the evening. After 4 weeks, the hair length and weight of the mice in the test area were measured.

[0102] Table 3 Test results of mouse hair length and hair weight

[0103] Hair length (mm) <![CDATA[Gross weight (mg / cm 2 ) <!-- 7 -->]]> Application Example 1 10.05 19.98 Application Example 3 12.41 22.59 Application Example 7 11.26 21.17 Blank group 9.09 17.6

[0104] Wheat germ extract contains unsaturated fatty acids, vitamins, and various minerals. It not only provides rich nutrients for hair growth and effectively maintains scalp elasticity, but also has antibacterial, repairing, and anti-hair loss effects on the hair and scalp.

[0105] Coralline algae extract is rich in marine enzymes, collagen, and minerals, including trace elements such as magnesium, zinc, manganese, and calcium. It replenishes trace elements for hair and can also act as a cell activator and bioreaction catalyst to solve the problem of hair loss caused by mineral deficiency.

[0106] Gynostemma pentaphyllum (Gynostemma pentaphyllum Mark) is a perennial vine in the Cucurbitaceae family, primarily found in the Yangtze River basin and southern Shaanxi. Rich in ginsenosides, which resemble ginsenosides, and richer in amino acids than ginseng, it is known as the "Southern Ginseng." It possesses both nutritional and therapeutic properties. Gynostemma pentaphyllum saponins can penetrate hair fibers and be absorbed by the hair. This is due to the multi-hydroxyl structure of the saponins forming hydrogen bonds with the multi-fatty groups in the hair fibers, connecting them. This increases the hair's strength and elongation, thereby increasing its toughness and resilience, making it less susceptible to breakage and loss. It also leaves hair translucent and shiny, while also removing grease and cleansing hair follicles, thereby improving hair loss.

[0107] Marine shellfish are rich in physiologically active substances such as proteins, peptides, amino acids, polysaccharides and trace elements; among them, bioactive peptides refer to peptide compounds that are beneficial to the life activities of biological organisms or have physiological effects. They are small molecule substances that can drive amino acids into hair follicles, stimulate scalp blood vessel proliferation, accelerate hair metabolism, and can effectively help hair growth and reduce hair loss.

[0108] The present invention adopts a rational combination of plant extracts and active peptides, so that they complement each other in the anti-hair loss mechanism and play a synergistic role. The composition of the present invention can provide necessary nutrients for each stage of hair growth, effectively repair scalp follicle damage, promote hair growth, nourish and strengthen hair, prevent hair loss, and effectively improve the scalp environment. It has a fast effect and a mild effect.

[0109] The present invention uses succinic anhydride to modify porous starch to enhance its adsorption capacity for active peptides; the porous starch containing active groups, carboxylated bacterial cellulose, and trimeryl glycol undergo an esterification reaction under the action of a catalyst, and simultaneously interact with the active groups in silk fibroin and porous starch to form a good coating layer for the active peptides; thereby alleviating the problem that the marine shellfish active peptides are easily inactivated by environmental factors such as temperature, oxygen, and humidity, thereby maintaining their original properties and improving their storage performance.

[0110] Test Example 3

[0111] The shampoo prepared in Application Example 3 was poured into a test tube, sealed, and placed in -5°C, 25°C, and 40°C environments for one month and 10 cycles, and the appearance was observed. One cycle was placed in -5°C and 40°C environments for 24 hours each, and the product appearance was observed.

[0112] The experimental results show that the shampoo does not change at -5℃, 25℃, and 40℃, nor does it change under a cyclic environment, and has good storage stability.

[0113] The present invention also conducted safety tests on the shampoos in each application example according to the acute skin irritation test method in the "Cosmetic Hygiene Standard" (2015 edition). The test results showed that there was no erythema or swelling on the surface of the test animals and mice after 24 hours. Therefore, it can be seen that the shampoo in the present invention is non-irritating to the skin and has high safety.

[0114] The above describes in detail the preferred embodiments of the present invention. It should be understood that those skilled in the art can make numerous modifications and variations based on the concepts of the present invention without inventive effort. Therefore, any technical solutions that can be derived by those skilled in the art through logical analysis, reasoning, or limited experimentation based on the concepts of the present invention and the prior art should be within the scope of protection defined by the claims.

Claims

1. An anti-hair loss composition based on plant extracts, characterized in that: Including modified active peptides and plant extracts; The mass ratio of the modified active peptide to the plant extract is (1-3): (1-3); The preparation method of the modified active peptide comprises the following steps: (1) Preparation of active peptides: Shell the marine shellfish and clean them, add 2-3 times the weight of water, homogenize for 1-10 minutes, then boil for 1-5 minutes, cool to room temperature, add enzyme preparation and hydrolyze for 30-60 minutes, finally inactivate the enzyme, centrifuge and take the supernatant, pass the supernatant through a 5000Da ultrafiltration membrane for ultrafiltration, and freeze-dry to obtain active peptides; (2) Add 10-20 parts by weight of porous starch to 100-120 parts by weight of water and stir evenly, heat to 30-40°C, add 1-5wt% of aqueous hydroxide solution to adjust the pH to 7.8-8.2, continue to maintain for 10-20 minutes, then add 3-8 parts by weight of succinic anhydride and stir evenly, heat to 30-40°C and react for 30-60 minutes, filter, wash and dry after the reaction to obtain modified porous starch; add 5-10 parts by weight of modified porous starch to 50-100 parts by weight of water and mix evenly, then add 1-7 parts by weight of active peptide, shake and adsorb at 30-35°C for 30-60 minutes; then add 2-7 parts by weight of carboxylated bacterial cellulose, 3-10 parts by weight of tripropylene glycol, 1-5 parts by weight of silk fibroin, 0.1-0.6 parts by weight of dibutyltin dilaurate, heat to 50-60°C and react for 30-60 minutes, spray dry after the reaction to obtain modified active peptide; The plant extract is at least one of wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract.

2. The anti-hair loss composition based on plant extracts according to claim 1, characterized in that: The plant extract is prepared by mixing wheat germ extract, coralline algae extract and Gynostemma pentaphyllum extract in a mass ratio of 2:1:

1.

3. The anti-hair loss composition based on plant extracts according to claim 1, characterized in that: The marine shellfish is at least one of oysters, paphia undulata, clams, scallops and pearl oysters.

4. The anti-hair loss composition based on plant extracts according to claim 1, characterized in that: The enzyme preparation is at least one of bromelain, neutral protease and pepsin.

5. Use of the anti-hair loss composition based on plant extracts according to any one of claims 1 to 4 in the preparation of scalp care products.

6. The use according to claim 5, characterized in that: The scalp care product is any one of shampoo, essence, conditioner, hair spray, and hair mask.

7. A shampoo, characterized in that: An anti-hair loss composition comprising the plant extract according to any one of claims 1 to 4.

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