Mixed feed additive containing bacillus subtilis and preparation method thereof

Abstract

The invention belongs to the technical field of livestock breeding additive products, and particularly relates to a mixed feed additive containing bacillus subtilis, which is prepared from the following ingredients in parts by weight: 5 to 10 parts of alpha-galactosidase, 1 to 5 parts of lysozyme, 1 to 5 parts of xylanase and 6 to 10 parts of glucose oxidase. Wherein the total amount of the bacillus subtilis, the active dry yeast and the bacillus licheniformis is 30-44 parts by weight; the mixed content of the vitamin A and the vitamin C is 2-10 parts by weight, the content of the calcium pantothenate is 2-6 parts by weight, and the content of the milk powder is 2-10 parts by weight. Compared with the prior art, the mixed feed additive is prepared by adding the bacillus subtilis into the mixed feed additive, and has the advantages of inhibiting the breeding of germs, improving the growth of beneficial bacteria, regulating the intestinal function, improving digestion and absorption and improving the utilization of intestinal tracts on nutrient substances. The invention also provides a preparation method of the mixed feed additive containing bacillus subtilis.

Description

Technical Field

[0001] The invention belongs to the technical field of livestock breeding additive products, and in particular relates to a mixed feed additive containing Bacillus subtilis and a preparation method thereof. Background Art

[0002] At present, livestock and poultry are mainly raised through feed. In the large-scale breeding cost, feed cost accounts for a very large proportion. If the cost of feed can be reduced, the cost of the entire breeding can be greatly reduced, so the digestion and absorption capacity of livestock and poultry is very important; Bacillus subtilis is a kind of Bacillus, widely distributed in soil and corrupt organic matter, and is named for its easy reproduction in the juice of dead grass. The single cell of this bacterium is (0.7-0.8) μm × (2-3) μm, and the color is uniform. There is no capsule, flagella, and it can move. Gram staining is positive, and the spores are (0.6-0.9) μm × (1.0-1.5) μm, located in the center of the bacterial body or slightly off, and the bacterial body does not swell after spore formation. The ingredients currently used in feed are relatively simple, and do not contain ingredients that promote the digestion and absorption capacity of the intestines of livestock and poultry, and do not have the function of inhibiting the growth of pathogens or regulating the intestines.

[0003] To this end, the present invention provides a mixed feed additive containing Bacillus subtilis and a preparation method thereof. Summary of the invention

[0004] In view of the above problems existing in the prior art, the object of the present invention is to provide a mixed feed additive containing Bacillus subtilis, which has the advantages of inhibiting the growth of pathogens, increasing the growth of beneficial bacteria, regulating intestinal function, improving digestion and absorption, and enhancing the utilization of nutrients by the intestine.

[0005] The purpose of the present invention can be achieved by the following technical solutions:

[0006] A mixed feed additive containing Bacillus subtilis, the additive is composed of the following parts by weight:

[0007] 5-10 parts by weight of α-galactosidase, 1-5 parts by weight of lysozyme, 1-5 parts by weight of xylanase, and 6-10 parts by weight of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast, and Bacillus licheniformis is 30-44 parts by weight; the mixed content of vitamin A and vitamin C is 2-10 parts by weight, the content of calcium pantothenate is 2-6 parts by weight, and the content of milk powder is 2-10 parts by weight.

[0008] As a preferred technical solution of the present invention, the mass ratio of the Bacillus subtilis, active dry yeast and Bacillus licheniformis is (0.5-1.4):(0.6-1.3):(1.1-3):(0.005-0.08).

[0009] As a preferred technical solution of the present invention, the mass ratio of the α-galactosidase, lysozyme, xylanase and glucose oxidase is 1:(0.8-1.2):(0.8-1.2):(0.1-0.5):(0.1-0.5).

[0010] As a preferred technical solution of the present invention, it also includes an antioxidant ethoxyquinoline, and the weight range of the antioxidant ethoxyquinoline is 0.5kg-2.0kg.

[0011] The present invention also provides a method for preparing a mixed feed additive containing Bacillus subtilis as described above, wherein the mixed feed additive is obtained by the following steps:

[0012] Step S1: Select rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation, and crushing, and crush them into 60 meshes for use as carriers;

[0013] Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis in a V-type mixer for 20 minutes;

[0014] Step S3: the rice husk powder and sepiolite crushed in step S1 are put into a double-blade mixer, and then the pre-mixed α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are put into the double-blade mixer from the small material feeding port and mixed thoroughly;

[0015] Step S4: adding 0.5kg-2.0kg of the antioxidant ethoxyquinoline into the mixer of step S3 and mixing for 80s to obtain the feed additive; the semi-finished product mixed evenly is directly placed into the silo of the packaging machine with the hatch opened for packaging.

[0016] As described above, the present invention relates to a mixed feed additive containing Bacillus subtilis and a preparation method thereof, which has the following beneficial effects:

[0017] After the present invention utilizes the above-mentioned mixed feed additive containing Bacillus subtilis, compared with the prior art, due to the use of such a structure, the mixed feed additive is prepared by adding Bacillus subtilis, and has the following advantages:

[0018] (1) It can inhibit the growth of pathogens and increase the growth of beneficial bacteria;

[0019] (2) It has the function of regulating the intestine, improving digestion and absorption, and enhancing the intestinal utilization of nutrients.

[0020] The present invention will be further described below in conjunction with specific implementation modes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The present invention is a flow chart of a method for preparing a mixed feed additive containing Bacillus subtilis;

[0022] Figure 2 This is a product diagram of a mixed feed additive containing Bacillus subtilis used for implementing the present invention;

[0023] Figure 3 This is a schematic diagram of the distribution of intestinal microbial flora of growing and fattening pigs as an example of feeding in Examples 1-3 of the present invention. DETAILED DESCRIPTION

[0024] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0025] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions that the present invention can be implemented, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the effects that the present invention can produce and the purposes that can be achieved. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. The change or adjustment of the relative relationship should also be regarded as the scope of the implementation of the present invention without substantial changes in the technical content. The specific structure can be described with reference to the drawings of the patent application.

[0026] The mixed feed additive containing Bacillus subtilis and the preparation method thereof applied by the present invention are taken as examples of livestock such as pigs in the following specific embodiments.

[0027] Comparative Example

[0028] 1. Ratio of raw materials

[0029] A mixed feed additive comprises the following raw materials by weight:

[0030] 5 parts of α-galactosidase, 5 parts of lysozyme, 3 parts of xylanase, and 5 parts of glucose oxidase; the total amount of active dry yeast and Bacillus licheniformis is 30 parts; the mixed content of vitamin A and vitamin C is 2 parts, the content of calcium pantothenate is 2 parts, and the content of milk powder is 2 parts.

[0031] 2. Preparation process

[0032] The preparation method of a mixed feed additive containing Bacillus subtilis comprises the following steps

[0033] Step S1: Select appropriate amount of rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation and crushing, and crush them into 60 meshes as carriers for standby use;

[0034] Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis prepared in Example 1 uniformly with a V-type mixer for 20 minutes;

[0035] Step S3: The rice husk powder and sepiolite crushed in the above step S1 are put into a double-blade mixer, and then α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are pre-mixed uniformly in Example 1 and put into the double-blade mixer from the small material feeding port, and fully mixed;

[0036] Step S4: Add 0.5 kg of antioxidant ethoxyquinoline into the mixer of step S3 and mix for 80 seconds to obtain the feed additive; directly open the hatch of the semi-finished product mixed evenly and put it into the silo of the packaging machine for packaging.

[0037] Example 1

[0038] 1. Ratio of raw materials

[0039] A mixed feed additive containing Bacillus subtilis comprises the following raw materials by weight:

[0040] 5 parts of α-galactosidase, 5 parts of lysozyme, 3 parts of xylanase, and 5 parts of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast, and Bacillus licheniformis is 30 parts; the mixed content of vitamin A and vitamin C is 2 parts, the content of calcium pantothenate is 2 parts, and the content of milk powder is 2 parts.

[0041] 2. Preparation process

[0042] Preparation method of mixed feed additive containing Bacillus subtilis, combined with Figure 1 As shown, the following steps are included

[0043] Step S1: Select appropriate amount of rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation and crushing, and crush them into 60 meshes as carriers for standby use;

[0044] Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis prepared in Example 1 uniformly with a V-type mixer for 20 minutes;

[0045] Step S3: The rice husk powder and sepiolite crushed in the above step S1 are put into a double-blade mixer, and then α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are pre-mixed uniformly in Example 1 and put into the double-blade mixer from the small material feeding port, and fully mixed;

[0046] Step S4: Add 0.5 kg of antioxidant ethoxyquinoline into the mixer of step S3 and mix for 80 seconds to obtain the feed additive; directly open the hatch of the semi-finished product mixed evenly and put it into the silo of the packaging machine for packaging.

[0047] 3. Usage:

[0048] The mixed feed additive of the present invention is mixed with feed, stirred evenly and then used. The feed additive (unit: g / ton) is formulated according to the following formulas for different pig growth types, and can be fed for a long time according to the effect of use.

[0049] Pregnant sow Lactating sows Piglets Growth and development of fat pigs 1000-1500 500-1000 1000 500-1000

[0050] Example 2

[0051] 1. Ratio of raw materials

[0052] A mixed feed additive containing Bacillus subtilis comprises the following raw materials by weight:

[0053] 8 parts of α-galactosidase, 2 parts of lysozyme, 5 parts of xylanase, and 10 parts of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast and Bacillus licheniformis is 40 parts; the mixed content of vitamin A and vitamin C is 5 parts, the content of calcium pantothenate is 5 parts, and the content of milk powder is 10 parts.

[0054] 2. Preparation process

[0055] Preparation method of mixed feed additive containing Bacillus subtilis, combined with Figure 1 As shown, the following steps are included

[0056] Step S1: Select appropriate amount of rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation and crushing, and crush them into 60 meshes as carriers for standby use;

[0057] Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis prepared in Example 1 uniformly with a V-type mixer for 20 minutes;

[0058] Step S3: The rice husk powder and sepiolite crushed in the above step S1 are put into a double-blade mixer, and then α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are pre-mixed uniformly in Example 1 and put into the double-blade mixer from the small material feeding port, and fully mixed;

[0059] Step S4: Add 1.0 kg of antioxidant ethoxyquinoline into the mixer of step S3 and mix for 80 seconds to obtain the feed additive; directly open the hatch of the semi-finished product mixed evenly and put it into the silo of the packaging machine for packaging.

[0060] 3. Usage:

[0061] The mixed feed additive of the present invention is mixed with feed, stirred evenly and then used. The feed additive (unit: g / ton) is formulated according to the following formulas for different pig growth types, and can be fed for a long time according to the effect of use.

[0062] Pregnant sow Lactating sows Piglets Growth and development of fat pigs 1000-1500 500-1000 1000 500-1000

[0063] Example 3

[0064] 1. Ratio of raw materials

[0065] A mixed feed additive containing Bacillus subtilis comprises the following raw materials by weight:

[0066] 10 parts of α-galactosidase, 5 parts of lysozyme, 5 parts of xylanase, and 10 parts of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast, and Bacillus licheniformis is 44 parts; the mixed content of vitamin A and vitamin C is 10 parts, the content of calcium pantothenate is 6 parts, and the content of milk powder is 8 parts.

[0067] 2. Preparation process

[0068] Preparation method of mixed feed additive containing Bacillus subtilis, combined with Figure 1 As shown, the following steps are included

[0069] Step S1: Select appropriate amount of rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation and crushing, and crush them into 60 meshes as carriers for standby use;

[0070] Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis prepared in Example 1 uniformly with a V-type mixer for 20 minutes;

[0071] Step S3: The rice husk powder and sepiolite crushed in the above step S1 are put into a double-blade mixer, and then α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are pre-mixed uniformly in Example 1 and put into the double-blade mixer from the small material feeding port, and fully mixed;

[0072] Step S4: Add 0.5 kg of antioxidant ethoxyquinoline into the mixer of step S3 and mix for 80 seconds to obtain the feed additive; directly open the hatch of the semi-finished product mixed evenly and put it into the silo of the packaging machine for packaging.

[0073] 3. Usage:

[0074] The mixed feed additive of the present invention is mixed with feed, stirred evenly and then used. The feed additive (unit: g / ton) is formulated according to the following formulas for different pig growth types, and can be fed for a long time according to the effect of use.

[0075] Pregnant sow Lactating sows Piglets Growth and development of fat pigs 1000-1500 500-1000 1000 500-1000

[0076] Combination Figure 2 As shown, the mixed feed additives produced in the comparative example, Example 1, Example 2 and Example 3 are mixed with the feed, stirred evenly and then used, and the growing fattening pigs are fed as an example, the feed mixed with the product has a specification of 800 g / ton, and the feed with the corresponding mixed feed additive is added according to the amount of 800 g / ton three times a day to feed the growing fattening pigs; Figure 3 is a schematic diagram of the distribution of intestinal microbial flora of growing and fattening pigs in the above three embodiments, see Figure 3 shown.

[0077] go through Figure 3 There was no significant difference in the sample coverage, number of observed species, Chao1 diversity index, and diversity index of intestinal absorption in the above intestinal microbial flora distribution map (p>0.05); the raw material ratio of Example 3, Figure 3The figure located in the lower right corner (i.e., the fourth figure) has an obvious distribution rate of beneficial bacteria (the distribution area of ​​bacteria is larger than that of other embodiments), that is, according to 10 parts of α-galactosidase, 5 parts of lysozyme, 5 parts of xylanase, and 10 parts of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast and Bacillus licheniformis is 44 parts; the mixed content of vitamin A and vitamin C is 10 parts, the content of calcium pantothenate is 6 parts, and the content of milk powder is 8 parts. The growth effect of pigs fed with mixed feed additives is the best; compared with the growing and fattening pigs fed with feed without adding mixed feed additives, the effect is obvious, which shows that the mixed feed additive produced by the present invention has the advantages of inhibiting the growth of pathogens, improving the growth of beneficial bacteria, regulating intestinal function, improving digestion and absorption, and enhancing the utilization of nutrients by the intestine.

[0078] In summary, the present invention effectively overcomes various shortcomings of the prior art and has high industrial utilization value.

[0079] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A mixed feed additive containing Bacillus subtilis, It is characterized in that The additive is composed of the following parts by weight: 5-10 parts by weight of α-galactosidase, 1-5 parts by weight of lysozyme, 1-5 parts by weight of xylanase, and 6-10 parts by weight of glucose oxidase; the total amount of Bacillus subtilis, active dry yeast, and Bacillus licheniformis is 30-44 parts by weight; the mixed content of vitamin A and vitamin C is 2-10 parts by weight, the content of calcium pantothenate is 2-6 parts by weight, and the content of milk powder is 2-10 parts by weight.

2. A mixed feed additive containing Bacillus subtilis as claimed in claim 1, Features: The mass ratio of the Bacillus subtilis, active dry yeast and Bacillus licheniformis is (0.5-1.4):(0.6-1.3):(1.1-3):(0.005-0.08).

3. A mixed feed additive containing Bacillus subtilis as claimed in claim 1, Features: The mass ratio of the α-galactosidase, lysozyme, xylanase and glucose oxidase is 1:(0.8-1.2):(0.8-1.2):(0.1-0.5):(0.1-0.5).

4. A mixed feed additive containing Bacillus subtilis as claimed in claim 1, Features: The antioxidant ethoxyquinoline is also included, and the weight range of the antioxidant ethoxyquinoline is 0.5kg-2.0kg.

5. A method for preparing a mixed feed additive containing Bacillus subtilis as claimed in any one of claims 1 to 4, wherein the mixed feed additive is obtained by the following steps: Step S1: Select rice husk powder and sepiolite as carriers, and process the carriers through three steps of primary selection, magnetic separation, and crushing, and crush them into 60 meshes for use as carriers; Step S2: premixing the α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis and Bacillus licheniformis in a V-type mixer for 20 minutes; Step S3: the rice husk powder and sepiolite crushed in step S1 are put into a double-blade mixer, and then the pre-mixed α-galactosidase, lysozyme, xylanase, glucose oxidase, Bacillus subtilis, Bacillus licheniformis, a mixture of vitamin A and vitamin C, calcium pantothenate, and milk powder are put into the double-blade mixer from the small material feeding port and mixed thoroughly; Step S4: adding 0.5kg-2.0kg of the antioxidant ethoxyquinoline into the mixer of step S3 and mixing for 80s to obtain the feed additive; the semi-finished product mixed evenly is directly placed into the silo of the packaging machine with the hatch opened for packaging.

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