Shenmai small-molecular oligopeptide with immunity improving effect and preparation method of Shenmai small-molecular oligopeptide

Abstract

The preparation method comprises the following steps: taking wheat oligopeptide as a main component, and adding gastrodia elata, eucommia ulmoides, poria cocos, peach kernels, uncaria, a ginseng extract, a cordyceps militaris extract, a dried ginger extract, a herba cepbalanoplosis segeti extract, maltodextrin, a sea-buckthorn extract, radix stephaniae tetrandrae and an apocynum venetum extract at the same time. The Shenmai micromolecular oligopeptide with the effect of improving immunity is prepared by specific preparation processes of extraction, dissolution, mixing, enzymolysis and the like.

Description

Technical Field

[0001] The invention relates to the field of health food, and in particular to a ginseng and wheat small molecule oligopeptide with the effect of improving immunity and a preparation method thereof. Background Art

[0002] As the pace of modern life accelerates, people are facing more and more health challenges, among which the maintenance of immunity has become the focus of public attention. Immunity refers to the body's ability to defend against external pathogens and is one of the key factors in maintaining health. In the field of health foods, products that enhance immunity have become a hot topic in research and development.

[0003] Existing health foods that improve immunity mainly achieve their effects by supplementing specific nutrients or bioactive ingredients. However, many existing products are deficient in the selection of ingredients, the scientificity of the formula, and the verification of efficacy. Especially in the field of traditional Chinese medicine, although there are many widely recognized herbs in traditional medicine that enhance immunity, the application of these herbs in modern health foods often lacks systematic scientific research and standardized production processes.

[0004] Health foods with Chinese herbal ingredients, with their unique pharmacological effects and long history of clinical application, provide new possibilities for improving immunity. For example, traditional herbs such as ginseng, cordyceps militaris, gastrodia elata, eucommia bark and peach kernel have been proven to have multiple physiological activities such as immune regulation, anti-fatigue and anti-oxidation. However, the application of these herbs in health foods is often limited by the dosage relationship, extraction process, stability, dosage control and the depth of integration with modern nutrition.

[0005] Wheat peptides (WPs) are a series of small molecule peptides obtained from wheat protein by enzymatic or acid hydrolysis. These oligopeptides have attracted extensive attention in the fields of food science and nutrition and health in recent years due to their unique biological activities and nutritional properties. Existing studies have shown that wheat oligopeptides have the following physiological effects: 1. Antioxidant effect; 2. Immunomodulatory function; 3. Anti-fatigue effect; 4. Promoting mineral absorption; 5. Lowering blood pressure; 6. Improving intestinal health, etc. Although wheat oligopeptides show potential health benefits in the above aspects, their application in the food industry still faces some challenges. For example, the dosage ratio control and preparation process of wheat oligopeptides in combination with other traditional Chinese medicine ingredients still need further research and optimization.

[0006] The present invention aims to solve the deficiencies in the prior art by conducting an in-depth study of the dosage ratio relationship when wheat oligopeptides are combined with other traditional Chinese medicine components and using a new preparation process to develop a new, scientifically proportioned health food with a clear effect of enhancing immunity. Summary of the invention

[0007] The technical problem to be solved by the present invention is to overcome the deficiencies of health foods in the prior art, especially the deficiencies of health foods for improving immunity in the prior art, and to provide a health food for improving immunity with a specific dosage ratio and a new preparation process. The health food is obtained through screening of a specific dosage ratio and a specific preparation process, and the effect of improving immunity is significantly enhanced and the bioavailability is significantly improved.

[0008] The combination provided by the present invention is as follows: The ginseng and wheat small molecule oligopeptide contains the following ingredients: wheat oligopeptide, Gastrodia elata, Eucommia ulmoides, Poria cocos, peach kernel, Uncaria rhynchophylla, ginseng extract, Cordyceps militaris extract, dried ginger extract, thistle extract, maltodextrin, sea buckthorn extract, Stephania tetrandra, and Apocynum venetum extract.

[0009] Among them, the main functions or effects of the above ingredients are as follows: Wheat oligopeptides: Wheat oligopeptides are small molecule proteins extracted from wheat, which are easy to digest and absorb. They can provide essential amino acids, help enhance immunity and promote muscle recovery.

[0010] Gastrodia elata: Gastrodia elata is a traditional Chinese medicinal material, mainly used to treat symptoms such as dizziness, headache, insomnia, etc. It has the effects of calming the liver and calming the wind, and tranquilizing the mind.

[0011] Eucommia: Eucommia has the effect of nourishing the liver and kidneys, strengthening the tendons and bones. It is often used to treat symptoms such as soreness of the waist and knees, weak tendons and bones, and it also has a certain effect in lowering blood pressure.

[0012] Poria cocos: Poria cocos is a medicinal material that can promote diuresis and eliminate dampness. It has the effects of strengthening the spleen and calming the nerves, promoting diuresis and reducing swelling. It is often used to treat symptoms such as edema, spleen deficiency and loss of appetite.

[0013] Peach kernel: Peach kernel has the effects of promoting blood circulation, removing blood stasis, moistening the intestines and promoting bowel movements. It is often used to treat symptoms such as pain and constipation caused by blood stasis.

[0014] Uncaria rhynchophylla: Uncaria rhynchophylla has the effects of calming the liver and calming wind, clearing away heat and calming the liver. It is often used to treat symptoms such as hypertension, headache, and dizziness.

[0015] Ginseng extract: Ginseng extract contains a variety of active ingredients and has many functions such as enhancing immunity, anti-fatigue, and anti-aging.

[0016] Cordyceps militaris extract: Cordyceps militaris is a precious Chinese herbal medicine that has the effects of nourishing the lungs and kidneys, relieving cough and asthma, and is also used to improve the body's immunity.

[0017] Dried ginger extract: Dried ginger has the effects of warming the middle and dispelling cold, restoring yang and rescuing adverse conditions. It is often used to treat symptoms such as spleen and stomach deficiency, abdominal pain and diarrhea.

[0018] Baby thistle extract: Baby thistle has the effects of clearing away heat and detoxifying, cooling blood and stopping bleeding, and is often used to treat symptoms such as fever and bleeding.

[0019] Maltodextrin: Maltodextrin is a carbohydrate that has the functions of providing energy and improving the taste of food. It is a common ingredient in health foods.

[0020] Sea buckthorn extract: Sea buckthorn is rich in vitamins and antioxidants, which have the effects of enhancing immunity and promoting healthy skin.

[0021] Stephania tetrandra: Also known as Hanfangji, it is the dried root of Stephania tetrandra S. Moore, a plant of the Menispermaceae family. It is a traditional Chinese medicinal material, mainly used to dispel wind and dampness, promote diuresis and reduce swelling. It has multiple pharmacological effects such as analgesia, anti-inflammatory, and blood pressure reduction. It is suitable for the treatment of rheumatic pain, edema, and difficulty urinating.

[0022] Luobu Ma Extract: Luobu Ma has the effects of clearing away heat and detoxifying, calming the liver and relieving wind, and is often used to treat symptoms such as hypertension, headache, and dizziness.

[0023] The composition and dosage ratio of ginseng and wheat small molecule oligopeptides are as follows: 30-70% wheat oligopeptides, 1-10% gastrodia elata, 1-10% eucommia ulmoides, 2-10% tuckahoe, 2-10% peach kernel, 2-10% Uncaria rhynchophylla, 2-10% ginseng extract, 1-10% cordyceps militaris extract, 2-10% dried ginger extract, 1-10% thistle extract, 1-10% maltodextrin, 5-20% seabuckthorn extract, 1-10% stephania tetrandra, and 1-10% apocynum venetum extract.

[0024] Furthermore, the composition and dosage ratio of the ginseng and wheat small molecule oligopeptides are: 50% wheat oligopeptides, 2% gastrodia elata, 3% eucommia ulmoides, 5% poria, 3% peach kernel, 3% Uncaria rhynchophylla, 5% ginseng extract, 2% cordyceps militaris extract, 5% dried ginger extract, 2% thistle extract, 2% maltodextrin, 10% sea buckthorn extract, 3% stephania tetrandra, and 5% dogbane extract.

[0025] Further, the preparation method and steps of ginseng and wheat small molecule oligopeptide are as follows: S1. Raw material preparation Wheat oligopeptide: prepare 50% wheat oligopeptide powder. Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, maltodextrin, Stephania tetrandra: prepare 2%, 3%, 3%, 3%, 5%, 2%, 3% of the corresponding raw materials respectively, and make sure they are dry and pure.

[0026] S2. Extraction process Ginseng extract, dried ginger extract, Apocynum venetum extract, Cordyceps militaris extract, Thistle extract, Sea buckthorn extract: extract the active ingredients in the ratio of 5%, 5%, 5%, 2%, 2%, and 10% using appropriate solvents and methods.

[0027] S3. Raw material crushing Solid raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra are crushed to the required fineness for easy mixing and dissolution.

[0028] S4. Mixed raw materials The crushed raw materials are mixed evenly with the wheat oligopeptide powder.

[0029] S5. Dissolving and mixing The extracted active ingredients are dissolved in a suitable amount of solvent, such as purified water, to form a solution.

[0030] S6. Mixing temperature control The above mixed powder was mixed with the extraction solution at a temperature of 40°C to ensure full dissolution and dispersion.

[0031] S7. Enzymatic hydrolysis Protease is added for enzymolysis, the temperature is controlled at 50°C, the pH value is controlled at 7.0-8.0, and the enzymolysis time is determined according to the characteristics of the enzyme used.

[0032] S8. Isolation and purification Insoluble impurities are removed using a centrifuge (e.g., a decanter centrifuge) and further purified by filtration (e.g., using a 200 nm pore size filter membrane).

[0033] S9. Concentration The mixture was concentrated using a vacuum concentrator to a Baume value of 20%.

[0034] S10. Drying A spray drying tower is used for drying, with the inlet temperature controlled at 160-180°C and the outlet temperature controlled at 60-80°C.

[0035] S11. Quality Inspection The dried powder is subjected to quality inspection, including ingredient ratio, active ingredient content, microbial indicators, etc.

[0036] S12. Packaging The qualified powder is packaged into sterile packaging.

[0037] S13. Storage and transportation Store the product in appropriate conditions and transport it as required.

[0038] Further, the above preparation process is refined as follows: Raw material pretreatment Wheat oligopeptides: Select high-purity wheat oligopeptide powder and sieve it through a sieving machine to ensure uniform particle size for subsequent mixing.

[0039] Chinese medicine raw materials: After cleaning and drying, the herbal raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra are crushed to less than 200 mesh using an ultra-fine grinder to increase the extraction rate of the active ingredients.

[0040] Extraction process Selection of extraction solvent: Choose a suitable extraction solvent such as ethanol or water according to the characteristics of the herb.

[0041] Extraction temperature: Set the extraction temperature to 60-80°C to ensure the dissolution of the active ingredients.

[0042] Extraction time: The extraction process lasts 2-4 hours to ensure that the active ingredients are fully extracted.

[0043] Mixing and dissolving Preliminary mixing: Mix the crushed herbal powder and wheat oligopeptide powder in a blender, with the mixing ratio being 2%-5% of the total mass of herbal powder and 50% of wheat oligopeptide.

[0044] Dissolution: Add the extracted herbal extract to the mixed powder in proportion and heat the mixture to 40°C using a constant temperature water bath or a heating and stirring device to facilitate dissolution.

[0045] Enzymatic hydrolysis process Enzyme selection: Choose a suitable protease, such as neutral protease or alkaline protease.

[0046] Enzymatic hydrolysis conditions: The enzymatic hydrolysis temperature is controlled at 45-55°C, the pH value is controlled at 7.0-8.5, and the enzymatic hydrolysis time is determined according to the characteristics of the enzyme used, generally 1-3 hours.

[0047] Separation and purification Centrifugal separation: Use a high-speed centrifuge to remove insoluble solid particles. The centrifugal speed and time are set according to the equipment performance.

[0048] Filtration: Use a ceramic membrane with a pore size of 200nm for microfiltration to remove large molecular impurities.

[0049] Concentration and drying Concentration: The mixture was concentrated using a triple-effect vacuum concentrator until the Baume value reached 20%.

[0050] Spray drying: Set the inlet temperature to 160-180°C and the outlet temperature to 60-80°C for spray drying to obtain a powdered product.

[0051] Quality Control Component analysis: Use high performance liquid chromatography (HPLC) and other techniques to quantitatively analyze the components in the product.

[0052] Microbiological testing: Ensures that products meet microbiological safety standards.

[0053] Packaging and storage Aseptic packaging: The product is packaged under aseptic conditions, using moisture-proof and light-proof packaging materials. The above can be packaged in 100g, 1000g, etc.

[0054] Storage conditions: The product should be stored in a cool, dry and dark environment. DETAILED DESCRIPTION

[0055] The present invention is further described below by specific examples and comparative examples. However, it should be understood that these examples and comparative examples are only used for more detailed and specific description and should not be construed as limiting the present invention in any form.

[0056] Example 1

[0057] Raw material composition: wheat oligopeptide 50%, Gastrodia elata 2%, Eucommia ulmoides 3%, Poria cocos 5%, Peach kernel 3%, Uncaria rhynchophylla 3%, Ginseng extract 5%, Cordyceps militaris extract 2%, Dry ginger extract 5%, Thistle extract 2%, Maltodextrin 2%, Seabuckthorn extract 10%, Stephania tetrandra 3%, Apocynum venetum extract 5%.

[0058] Preparation process: S1. Raw material preparation Wheat oligopeptide: prepare 50% wheat oligopeptide powder. Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, maltodextrin, Stephania tetrandra: prepare 2%, 3%, 3%, 3%, 5%, 2%, 3% of the corresponding raw materials respectively, and make sure they are dry and pure.

[0059] S2. Extraction process Ginseng extract, dried ginger extract, Apocynum venetum extract, Cordyceps militaris extract, Thistle extract, Sea buckthorn extract: in the ratio of 5%, 5%, 5%, 2%, 2%, 10%, using ethanol concentration of about 60%, extraction temperature of 60°C, and extraction time of 3 hours.

[0060] S3. Raw material crushing Solid raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra are crushed using an ultrafine grinder to below 200 meshes to achieve the required fineness for easy mixing and dissolution.

[0061] S4. Mixed raw materials The crushed raw materials are mixed evenly with the wheat oligopeptide powder.

[0062] S5. Dissolving and mixing The extracted herbal extract is added to the mixed powder in proportion.

[0063] S6. Mixing temperature control The above mixed powder was mixed with the extraction solution at a temperature of 40°C to ensure full dissolution and dispersion.

[0064] S7. Enzymatic hydrolysis Protease is added for enzymolysis, neutral protease or alkaline protease can be used. In this embodiment, neutral protease is used, the temperature is controlled at 50°C, the pH value is controlled at 7.0-8.0, and the enzymolysis time is 1.5 hours.

[0065] S8. Isolation and purification A centrifuge (in this example, a horizontal screw centrifuge) is used to remove insoluble impurities, and then a ceramic membrane with a pore size of 200 nm is used for microfiltration to remove macromolecular impurities.

[0066] S9. Concentration The mixture was concentrated using a triple-effect vacuum concentrator until the Baume value reached 20%.

[0067] S10. Drying A spray drying tower is used for drying, with the inlet temperature controlled at 160-180°C and the outlet temperature controlled at 60-80°C to obtain a powdered product.

[0068] Example 2

[0069] Experimental purpose: Mainly to investigate and screen the effects of different dosage ratios on enhancing immunity.

[0070] Experimental drug 1: A ginseng and wheat small molecule oligopeptide prepared using the raw materials and proportions of the above-mentioned Example 1, wherein the preparation process is exactly the same as that of Example 1.

[0071] Experimental drug 2: Raw material composition: wheat oligopeptide 10%, Gastrodia elata 3.6%, Eucommia ulmoides 5.4%, Poria 9%, Peach kernel 5.4%, Uncaria rhynchophylla 5.4%, Ginseng extract 9%, Cordyceps militaris extract 3.6%, Dry ginger extract 9%, Thistle extract 3.6%, Maltodextrin 3.6%, Hippophae rhamnoides extract 18%, Stephania tetrandra 5.4%, Apocynum venetum extract 9%. Its preparation process is exactly the same as that of Example 1.

[0072] Experimental drug 3: wheat oligopeptide 80%, Gastrodia elata 0.8%, Eucommia ulmoides 1.2%, Poria cocos 2%, Peach kernel 1.2%, Uncaria rhynchophylla 1.2%, Ginseng extract 2%, Cordyceps militaris extract 0.8%, Dry ginger extract 2%, Thistle extract 0.8%, Maltodextrin 0.8%, Seabuckthorn extract 4%, Stephania tetrandra 1.2%, Apocynum venetum extract 2%.

[0073] Experimental requirements: The following requirements and steps for the immunity enhancement experiment are all in accordance with the requirements of the Health Food Function Test and Evaluation Method (2023 Edition) to conduct the product health function evaluation experiment.

[0074] Effect experiment 1: Delayed-type hypersensitivity (DTH) Experimental animals: 40 male SPF KM mice of the same sex, weighing between 18-22 g, were selected and randomly divided into 4 groups, with 10 mice in each group.

[0075] Preparation of drugs: Take 0.3g of the powder prepared according to the above experimental drugs 1-3, dissolve it in 10ml of pure water, and set aside; at the same time, use 10ml of normal saline for the negative control group. Administer by gavage once a day at noon for one month.

[0076] Sensitization: Mice were immunized with 2% (v / v) SRBC by intraperitoneal or intravenous injection, with 0.2 mL injected per mouse.

[0077] Production and determination of DTH: 4 days after immunization, the thickness of the left hind foot was measured, and then 20% (v / v) SRBC was injected subcutaneously at the measurement site, 20 μL (about 1×10 8 SRBC) was injected and the thickness of the left hind foot was measured 24 hours after injection. The same part was measured three times and the average value was taken. The difference in the thickness of the foot before and after the attack represented the degree of delayed-type hypersensitivity (DTH). The experimental results are shown in Table 1.

[0078] Table 1 Delayed-type hypersensitivity (DTH) of drugs in each group (x̄±SD, n=10)

[0079] As shown in Table 1, after intragastric administration of different dosage ratios of experimental drugs 1-3 and normal saline, the swelling degree of experimental drugs 2-3 was higher than that of the normal saline group (P<0.05), the swelling degree of experimental drug 1 was significantly higher than that of the normal saline group (P<0.01), and the swelling degree of experimental drug 1 was higher than that of experimental drugs 2-3 (P<0.05).

[0080] It can be seen that the combination of the present invention has an enhancing effect on the delayed-type allergic reaction ability of mice, and the combination of the present invention with a specific dosage ratio has a significant enhancing effect on the delayed-type allergic reaction ability of mice.

[0081] Effect experiment 2: ConA-induced mouse spleen lymphocyte transformation experiment Experimental animals: 40 male SPF KM mice of the same sex, weighing between 18-22 g, were selected and randomly divided into 4 groups, with 10 mice in each group.

[0082] Preparation of drugs: Take 0.3g of the powder prepared according to the above experimental drugs 1-3, dissolve it in 10ml of pure water, and set aside; at the same time, use 10ml of normal saline for the negative control group. Administer by gavage once a day at noon for one month.

[0083] Preparation of spleen cell suspension: Aseptically remove the spleen and place it in a plate containing an appropriate amount of sterile Hank's solution. Use forceps to gently grind the spleen to make a single cell suspension. Filter through a 200-mesh sieve or grind the spleen with 4 layers of gauze, wash twice with Hank's solution, and centrifuge for 10 minutes each time (1000r / min). Then suspend the cells in 1mL of complete culture medium, count the number of live cells with trypan blue staining (should be above 95%), and adjust the cell concentration to 3×10 6 Pieces / m.

[0084] Lymphocyte proliferation reaction: Each spleen cell suspension was added to two wells of a 24-well culture plate, 1 mL per well, 75 μL ConA solution (equivalent to 7.5 μg / mL) was added to one well, and the other well was used as a control. The cells were cultured in a 5% CO2, 37°C CO2 incubator for 72 hours. 4 hours before the end of the culture, 0.7 mL of supernatant was gently aspirated from each well, 0.7 mL of RPMI1640 culture solution without calf serum was added, and 50 μL of MTT (5 mg / mL) was added per well, and the culture was continued for 4 hours. After the culture was completed, 1 mL of acidic isopropanol was added to each well, and the mixture was mixed by blowing to completely dissolve the purple crystals. The cells were then dispensed into a 96-well culture plate, and 3 parallel wells were made for each well. The optical density value was measured at a wavelength of 570 nm using an enzyme-labeled instrument. The dissolved solution can also be directly transferred into a 2 mL cuvette, and the OD value can be measured at a wavelength of 570 nm on a 721 spectrophotometer.

[0085] The optical density value of the well with ConA added minus the optical density value of the well without ConA added represents the proliferation capacity of lymphocytes. The experimental results are shown in Table 2.

[0086] Table 2: ConA-induced mouse spleen lymphocyte transformation experiment of each group of drugs (x̄±SD, n=10)

[0087] It can be seen that the combination of the present invention can improve the proliferation ability of lymphocytes, wherein the lymphocyte proliferation ability of experimental drug 1 is significantly stronger than that of the normal saline group, and is also stronger than that of experimental drug 2 and experimental drug 3.

[0088] In summary, the ginseng and wheat small molecule oligopeptide with a specific composition and dosage ratio prepared by a specific process of the present invention has the effect of significantly improving immunity.

[0089] Without departing from the scope of the present invention, those of ordinary skill in the art may implement modifications and variations to the present invention. In addition, it should be understood that the aspects of the various embodiments may be interchangeable in whole or in part. The foregoing description is exemplary only and is not intended to limit the present invention further described in the appended claims.

Claims

1. A ginseng and wheat small molecule oligopeptide with the effect of improving immunity, the composition and dosage ratio are: 30-70% wheat oligopeptide, 1-10% gastrodia elata, 1-10% eucommia ulmoides, 2-10% tuckahoe, 2-10% peach kernel, 2-10% Uncaria rhynchophylla, 2-10% ginseng extract, 1-10% cordyceps militaris extract, 2-10% dried ginger extract, 1-10% thistle extract, 1-10% maltodextrin, 5-20% seabuckthorn extract, 1-10% stephania tetrandra, and 1-10% apocynum venetum extract.

2. The ginseng and wheat small molecule oligopeptide as claimed in claim 1, wherein the composition and dosage ratio are: 50% wheat oligopeptide, 2% gastrodia elata, 3% eucommia bark, 5% poria, 3% peach kernel, 3% Uncaria rhynchophylla, 5% ginseng extract, 2% cordyceps militaris extract, 5% dried ginger extract, 2% thistle extract, 2% maltodextrin, 10% seabuckthorn extract, 3% stephania tetrandra, and 5% apocynum venetum extract.

3. The ginseng and wheat small molecule oligopeptide as claimed in claim 1 or 2, wherein the preparation steps are: S1. Raw material preparation Wheat oligopeptide: prepare 50% wheat oligopeptide powder, Gastrodia elata, Eucommia ulmoides, Persica chinensis, Uncaria rhynchophylla, Poria cocos, maltodextrin, Stephania tetrandra: prepare 2%, 3%, 3%, 3%, 5%, 2%, 3% of the corresponding raw materials respectively, and make sure they are dry and pure; S2. Extraction process Ginseng extract, dried ginger extract, Apocynum venetum extract, Cordyceps militaris extract, Thistle extract, Sea buckthorn extract: extract the active ingredients in the proportions of 5%, 5%, 5%, 2%, 2%, and 10% using appropriate solvents and methods; S3. Raw material crushing Grinding solid raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra to a desired fineness for easy mixing and dissolution; S4. Mixed raw materials Mix the crushed raw materials and wheat oligopeptide powder evenly; S5. Dissolving and mixing Dissolving the extracted active ingredients in a suitable amount of solvent, such as purified water, to form a solution; S6. Mixing temperature control The mixed powder was mixed with the extraction solution at 40°C to ensure full dissolution and dispersion; S7. Enzymatic hydrolysis Add protease for enzymolysis, control the temperature at 50°C, the pH value at 7.0-8.0, and the enzymolysis time is determined according to the characteristics of the enzyme used; S8. Separation and purification Use a centrifuge (e.g., a decanter centrifuge) to remove insoluble impurities, and then further purify by filtration (e.g., using a filter membrane with a pore size of 200 nm); S9. Concentration The mixed solution was concentrated using a vacuum concentrator to a Baume value of 20%; S10. Drying Use a spray drying tower for drying, with the inlet temperature controlled at 160-180°C and the outlet temperature controlled at 60-80°C; S11.Quality Inspection Conduct quality inspection on the dried powder, including ingredient ratio, active ingredient content, microbial indicators, etc.; S12. Packaging Pack qualified powder into sterile packaging; S13. Storage and transportation Store the product in appropriate conditions and transport it as required.

4. The ginseng and wheat small molecule oligopeptide as claimed in claim 3, wherein the preparation steps are: raw material pretreatment Wheat oligopeptide: Select high-purity wheat oligopeptide powder and sieve it through a sieving machine to ensure uniform particle size for subsequent mixing; Chinese medicine raw materials: After cleaning and drying, the herbal raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra are crushed to less than 200 meshes using an ultra-fine grinder to increase the extraction rate of effective ingredients; Extraction process Selection of extraction solvent: Choose a suitable extraction solvent, such as ethanol or water, according to the characteristics of the herb; Extraction temperature: Set the extraction temperature to 60-80°C to ensure the dissolution of the active ingredients; Extraction time: The extraction process lasts 2-4 hours to ensure that the active ingredients are fully extracted; Mixing and dissolving Preliminary mixing: Mix the crushed herbal powder and wheat oligopeptide powder in a blender, with the mixing ratio of herbal powder accounting for 2%-5% of the total mass and wheat oligopeptide accounting for 50%; Dissolution: Add the extracted herbal extract to the mixed powder in proportion, and heat the mixture to 40°C using a constant temperature water bath or a heating and stirring device to promote dissolution; Enzymatic hydrolysis process Enzyme selection: Choose a suitable protease, such as neutral protease or alkaline protease.

5. Enzymolysis conditions: The enzymolysis temperature is controlled at 45-55°C, the pH value is controlled at 7.0-8.5, and the enzymolysis time is determined according to the characteristics of the enzyme used, generally 1-3 hours; Separation and purification Centrifugal separation: Use a high-speed centrifuge to remove insoluble solid particles. The centrifugal speed and time are set according to the equipment performance; Filtration: Use a ceramic membrane with a pore size of 200nm for microfiltration to remove large molecular impurities.

6. Concentration and drying Concentration: Use a triple-effect vacuum concentrator to concentrate the mixture until the Baume value reaches 20%; Spray drying: Set the inlet temperature to 160-180°C and the outlet temperature to 60-80°C for spray drying to obtain a powdered product; Quality Control Component analysis: Use high performance liquid chromatography (HPLC) and other techniques to quantitatively analyze the components in the product; Microbiological testing: ensuring that products meet microbiological safety standards; Packaging and storage Aseptic packaging: product packaging is carried out under aseptic conditions, using moisture-proof and light-proof packaging materials. The above can be made into packaging of 100g, 1000g, etc.; Storage conditions: The product should be stored in a cool, dry and dark environment.

7. The ginseng and wheat small molecule oligopeptide as claimed in claim 3 or 4, wherein the preparation steps are: S1. Raw material preparation Wheat oligopeptide: prepare 50% wheat oligopeptide powder, Gastrodia elata, Eucommia ulmoides, Persica chinensis, Uncaria rhynchophylla, Poria cocos, maltodextrin, Stephania tetrandra: prepare 2%, 3%, 3%, 3%, 5%, 2%, 3% of the corresponding raw materials respectively, and make sure they are dry and pure; S2. Extraction process Ginseng extract, dried ginger extract, Apocynum venetum extract, Cordyceps militaris extract, Thistle extract, Seabuckthorn extract: in the proportion of 5%, 5%, 5%, 2%, 2%, 10%, use 60% ethanol, extraction temperature 60℃, extraction time 3 hours; S3. Raw material crushing Grind the solid raw materials such as Gastrodia elata, Eucommia ulmoides, peach kernel, Uncaria rhynchophylla, Poria cocos, and Stephania tetrandra using an ultrafine grinder to a fineness below 200 meshes to achieve the required fineness for easy mixing and dissolution; S4. Mixed raw materials Mix the crushed raw materials and wheat oligopeptide powder evenly; S5. Dissolving and mixing Adding the extracted herbal extract into the mixed powder in proportion; S6. Mixing temperature control The mixed powder was mixed with the extraction solution at 40°C to ensure full dissolution and dispersion; S7. Enzymatic hydrolysis Add protease for enzymolysis, neutral protease or alkaline protease can be used. In this embodiment, neutral protease is used, the temperature is controlled at 50° C., the pH value is controlled at 7.0-8.0, and the enzymolysis time is 1.5 hours; S8. Separation and purification A decanter centrifuge was used to remove insoluble impurities, and then a ceramic membrane with a pore size of 200 nm was used for microfiltration to remove macromolecular impurities; S9. Concentration The mixture was concentrated using a triple-effect vacuum concentrator until the Baume value reached 20%; S10. Drying Use a spray drying tower for drying, control the inlet temperature at 160-180°C, and the outlet temperature at 60-80°C to obtain a powdered product; S11.Quality Inspection Conduct quality inspection on the dried powder, including ingredient ratio, active ingredient content, microbial indicators, etc.; S12. Packaging Pack qualified powder into sterile packaging; S13. Storage and transportation Store the product in appropriate conditions and transport it as required.

8. A method for preparing a ginseng and wheat small molecule oligopeptide having the effect of improving immunity, wherein the specific preparation process and parameters are as described in any one of claims 3-5.

9. Use of the ginseng and wheat small molecule oligopeptide according to any one of claims 1 to 5 in the preparation of health food for enhancing immunity.

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