Application of poria cocos and peach composition in preparation of anti-fatigue, anti-hypoxia and exercise ability improving products

Abstract

The invention discloses application of a rhizoma smilacis glabrae and radix fici simplicissimae composition in preparation of anti-fatigue, anti-hypoxia and exercise ability improving products, and the rhizoma smilacis glabrae and radix fici simplicissimae composition is prepared by the following steps: 1) taking rhizoma smilacis glabrae and radix fici simplicissimae as raw materials, adding purified water which is 7-12 times of the mass of the raw materials, soaking for 2-3 hours, heating and boiling, condensing and refluxing for 1.5-2 hours, and filtering to obtain an extracting solution a and filter residues a; adding purified water of which the mass is 4-6 times that of the filter residues a into the filter residues a, heating and boiling, condensing and refluxing for 1.5-2 hours, and filtering to obtain an extracting solution b and filter residues b; combining the extracting solution a and the extracting solution b, concentrating and freeze-drying to obtain the poria cocos and peach composition. Experiments prove that the poria cocos and peach composition has the effects of resisting fatigue and oxygen deficit, enhancing anaerobic exercise ability and improving aerobic endurance quality. The poria cocos and peach composition disclosed by the invention can be applied to products such as medicines or foods for resisting fatigue, resisting hypoxia and improving exercise ability.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to the application of a Lingtao composition in preparing products for resisting fatigue, resisting hypoxia and improving exercise ability. Background Art

[0002] With the accelerated pace of life and increased work pressure in modern society, people are paying more and more attention to their own health and physical functions. Fatigue and hypoxia have become important factors affecting people's quality of life and work efficiency. Long-term fatigue will not only lead to a decline in physical function, but may also cause a series of chronic diseases. In some special environments, such as plateau areas or high-intensity work, hypoxia can also have a serious impact on the human body, limiting people's athletic ability and normal physiological functions. Traditional anti-fatigue and anti-hypoxia methods include rest, nutritional supplements and drug treatment. However, although rest and nutritional supplements are important, they are not ideal for some people who are under high pressure and high-intensity work for a long time. Drug treatment may bring some side effects, and the long-term effect cannot be expected. At the same time, in the field of sports, athletes and fitness enthusiasts are increasingly in urgent need of improving athletic ability and anti-fatigue and anti-hypoxia. At present, the types of sports nutrition supplements on the market are also relatively complicated, and the effects and safety of most of them still need to be clarified. Therefore, it is of great practical significance to develop safe, effective and side-free anti-fatigue, anti-hypoxia and athletic ability-enhancing products.

[0003] In summary, the research on products that fight fatigue, fight hypoxia and improve athletic ability has broad market prospects and social needs. Further in-depth research and exploration will help develop more efficient, safe and convenient products to meet people's needs for improved health and athletic ability. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art and provide an application of a Lingtao composition in the preparation of products for resisting fatigue, resisting hypoxia and improving athletic ability.

[0005] The technical solution of the present invention is summarized as follows

[0006] Application of a Lingtao composition in the preparation of products for resisting fatigue, resisting hypoxia and improving athletic ability, wherein the Lingtao composition is prepared by the following method:

[0007] 1) taking Smilax glabra and Prunus mume as raw materials in a mass ratio of 1:(0.8-1), adding purified water equivalent to 7-12 times the mass of the raw materials, soaking for 2-3 hours, heating to boil, condensing and refluxing for 1.5-2 hours, filtering, and obtaining an extract a and a residue a;

[0008] 2) adding purified water equivalent to 4-6 times the mass of the residue a to the residue a, heating and boiling, condensing and refluxing for 1.5-2h, filtering to obtain an extract b and a residue b; combining the extract a and the extract b, concentrating, and freeze-drying to obtain a Lingtao composition.

[0009] Advantages of the present invention:

[0010] Experiments have shown that the Lingtao combination has the effects of increasing the body's resistance to fatigue and hypoxia, strengthening muscle strength, enhancing anaerobic exercise capacity and improving aerobic endurance, thereby enhancing the user's athletic ability. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is the effect of the Lingtao composition prepared in Example 1 on the exhausted swimming time of mice. In the figure: *p<0.05, **p<0.01, **p<0.001 vs. solvent group (normal saline group).

[0012] Figure 2 This is the effect of the Lingtao composition prepared in Example 1 on the exhaustive running time of mice. In the figure: *p<0.05 vs. solvent group.

[0013] Figure 3 This is the effect of the Lingtao composition prepared in Example 1 on the physiological adaptability of mice induced by hypoxia. In the figure: *p<0.05, **p<0.001, vs. solvent group.

[0014] Figure 4 The effect of the Lingtao composition prepared in Example 1 on the gripping force of the forelimb muscles of mice, in the figure: *p<0.05,

[0015] **p<0.01, **p<0.001 vs. vehicle group.

[0016] Figure 5 This is the effect of the Lingtao composition prepared in Example 1 on the exercise metabolism level of mice. In the figure: *p<0.05, **p<0.001, vs. solvent group. DETAILED DESCRIPTION

[0017] The Lingtao composition of the present invention is made of Smilax glabra and Prunus mume as raw materials. Among them, the medicinal material Smilax glabra is the dried tuber of Smilax glabra of the genus Smilax of the Liliaceae family, which is sweet and light in taste, neutral in nature, and enters the liver and stomach meridians; it can detoxify, remove dampness, and open up joints.

[0018] The medicinal herb Prunus mume is the root of the Ficus microcarpa plant of the Moraceae family. It tastes sweet, is neutral in nature, and enters the spleen, lung, and liver meridians. It has the effects of invigorating qi and replenishing deficiency, promoting qi and relieving depression, strengthening tendons and activating collaterals, strengthening the spleen and eliminating dampness, and relieving cough and reducing phlegm.

[0019] The present invention will be further described below by means of specific examples.

[0020] Example 1

[0021] The Lingtao composite is prepared by the following method:

[0022] 1) Taking Smilax glabra and Prunus mume as raw materials in a mass ratio of 1:1, adding purified water equivalent to 10 times the mass of the raw materials, soaking for 2 hours, heating and boiling, condensing and refluxing for 2 hours, filtering, and obtaining an extract a and a residue a;

[0023] 2) adding pure water equivalent to 5 times the mass of the residue a to the residue a obtained in step 1), heating and boiling, condensing and refluxing for 2 hours, filtering to obtain an extract b and a residue b; combining the extract a and the extract b, concentrating, and freeze-drying to obtain a Lingtao composition.

[0024] Example 2

[0025] The Lingtao composite is prepared by the following method:

[0026] 1) Taking Smilax glabra and Prunus mume as raw materials in a mass ratio of 1:0.8, adding purified water equivalent to 12 times the mass of the raw materials, soaking for 2.5 hours, heating to boil, condensing and refluxing for 1.5 hours, filtering, and obtaining an extract a and a residue a;

[0027] 2) adding purified water equivalent to 4 times the mass of the residue a to the residue a obtained in step 1), heating and boiling, condensing and refluxing for 2 hours, filtering to obtain an extract b and a residue b; combining the extract a and the extract b, concentrating, and freeze-drying to obtain a Lingtao composition.

[0028] Example 3

[0029] The Lingtao composite is prepared by the following method:

[0030] 1) Taking Smilax glabra and Prunus mume as raw materials in a mass ratio of 1:0.9, adding purified water equivalent to 7 times the mass of the raw materials, soaking for 3 hours, heating to boil, condensing and refluxing for 1.8 hours, filtering, and obtaining an extract a and a residue a;

[0031] 2) adding purified water equivalent to 6 times the mass of the residue a to the residue a obtained in step 1), heating and boiling, condensing and refluxing for 1.5 hours, filtering to obtain an extract b and a residue b; combining the extract a and the extract b, concentrating, and freeze-drying to obtain a Lingtao composition.

[0032] Example 4

[0033] Anti-fatigue experiments (exhaustive swimming, exhaustive running and ten-minute swimming tests) of the Lingtao composition.

[0034] 1. Experimental materials:

[0035] 1.1 Lingtao composition: The Lingtao composition prepared in Example 1 was dissolved in physiological saline into low, medium and high doses (120 mg / kg body weight, 240 mg / kg body weight, 360 mg / kg body weight), stored at 4°C after preparation, and preheated to 37°C before each gavage.

[0036] 1.2 Experimental animals: Male SPF-grade C57BL / 6J mice, 8 weeks old, weighing 22 g to 24 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd., with certificate number: 210726240101079667. The breeding conditions were a temperature of 23 ± 2 °C and a relative humidity of 50% ± 10%. During the experiment, the mice had free access to food and water.

[0037] 1.3 Other experimental materials: Tribromoethanol, analytical grade, Shanghai BiDe Pharmaceutical Technology Co., Ltd.

[0038] 2. Experimental methods:

[0039] 28 male C57BL / 6J mice were randomly divided into 4 groups, 7 mice in each group, including solvent group (normal saline group), low-dose group (120 mg / kg body weight), medium-dose group (240 mg / kg body weight), and high-dose group (360 mg / kg body weight). Different drugs were continuously gavaged for 28 days, once a day.

[0040] 2.1 Exhaustive swimming test: Each mouse was loaded with a lead wire of 5% of its body weight on its tail and placed in a 40cm deep swimming pool (65cm high × 20cm diameter) at a water temperature of 34℃+1℃. The swimming assessment value was the time from when the mouse was placed in the water to exhaustion. The exhaustion time point was determined by observing that the mouse showed irregular movements and could not keep its body on the water for a time equal to or greater than 7s.

[0041] 2.2 Exhaustive running experiment: Before the experiment, all mice underwent 3 consecutive days of adaptive training. The treadmill conditions were set at 6m / min or 10m / min, 0° inclination, and the mice ran continuously for 10 minutes. On the day of the experiment, the mice began to fast 4 hours before getting on the treadmill. During the experiment, the treadmill inclination angle was 10°, and the treadmill speed was set to gradient acceleration. The initial speed was 6m / min, and the speed increased by 1m / min every 2 minutes until the speed reached 12m / min. Exhaustion of mice was manifested by their inability to resume movement by taking a short rest or gently tapping with a tongue depressor.

[0042] 2.3 Ten-minute swimming test: Before the experiment, mice were fasted for 4 hours (under fasting conditions, fatigue-related indicators during acute exercise can reflect the true physiological regulation ability). The tails of mice were loaded with a lead wire that was 5% of their body weight and placed in a 40cm deep swimming pool (65cm high × 20cm diameter) at a water temperature of 34℃±1℃ for 10 minutes of forced swimming. Real-time blood lactate concentration was measured before, after, and 30 minutes after the end of swimming.

[0043] 3. Experimental results:

[0044] 3.1 Effect of Lingtao Composition on Exhaustive Swimming Time in Anti-fatigue Experiment of Mice

[0045] Compared with the saline group, each dose group could significantly prolong the exhaustive swimming time of weight-bearing mice. Figure 1 This indicates that the Lingtao combination has a strong effect in resisting fatigue and improving exercise ability. Figure 1 .

[0046] 3.2 Effect of Lingtao Composition on Exhaustive Running Time in Anti-fatigue Experiment of Mice

[0047] Compared with the saline group, the high-dose group could significantly prolong the exhaustive running time of mice. Figure 2 , indicating that the Lingtao composition has the effect of resisting fatigue and improving exercise ability ( Figure 2 ).

[0048] 3.3 Effect of Lingtao Composition on Lactic Acid Levels in Mice after Ten Minutes of Forced Swimming in Anti-fatigue Experiment

[0049] Compared with the saline group, there was no difference in blood lactate of mice in each dose group before forced swimming; after forced swimming, the lactate of each dose group was lower than that of the solvent group; after resting for 30 minutes after swimming, the lactate of each dose group was significantly lower than that of the solvent group; after resting for 30 minutes after swimming, the blood lactate clearance rate of each dose group was significantly increased, as shown in Table 1.

[0050] Table 1. Effect of Lingtao composition on blood lactate in mice after 10 minutes of forced swimming

[0051]

[0052] The data are shown as mean ± SD. *p < 0.05, **p < 0.01, ****p < 0.001 vs. the vehicle group.

[0053] The above results show that the Lingtao combination has a strong anti-fatigue ability.

[0054] Example 5

[0055] Experiment on the Hypoxia Chamber of Lingtao Combination

[0056] 1. Experimental materials:

[0057] 1.1 Lingtao composition: The Lingtao composition prepared in Example 1 was dissolved with physiological saline to form a high-dose Lingtao composition (360 mg / kg body weight), which was stored at 4°C after preparation and preheated to 37°C before each intragastric administration.

[0058] 1.2 Experimental animals: Male SPF-grade C57BL / 6J mice, 8 weeks old, weighing 22 g to 24 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd., with certificate number: 210726240101079667. The breeding conditions were a temperature of 23 ± 2 °C and a relative humidity of 50% ± 10%. During the experiment, the mice had free access to food and water.

[0059] 1.3 Other experimental materials: Tribromoethanol, analytical grade, Shanghai BiDe Pharmaceutical Technology Co., Ltd.

[0060] 2. Experimental methods:

[0061] Fourteen male C57BL / 6J mice were randomly divided into two groups, 7 mice in each group, namely solvent group (normal saline group) and high-dose group (360 mg / kg body weight). They were gavaged once a day for 28 consecutive days.

[0062] A hypoxic chamber (CJ-D02245, Changsha Changjin Technology Co., Ltd.) was used to simulate hypoxia in mice. The oxygen concentration in the hypoxic chamber was set to 8%. In the hypoxic mode, mice entered the hypoxic chamber at 17:00 and left the hypoxic chamber at 17:00 the next day, staying in the hypoxic state for 24 hours. The real-time blood lactate concentration of mice was measured before entering the hypoxic chamber, when leaving the hypoxic chamber, and 30 minutes after leaving the hypoxic chamber ( Figure 3 ).

[0063] 3. Experimental results:

[0064] Figure 3 A. Blood lactate production rate of mice treated with high-dose Lingtao combination 24 hours after entering the hypoxia chamber; B. Blood lactate clearance rate of mice treated with high-dose Lingtao combination 30 minutes after leaving the hypoxia chamber. Data are mean ± standard deviation. *p<0.05, **p<0.001, vs. solvent group.

[0065] Compared with the solvent group, the elevated blood lactate concentration in the high-dose Lingtao combination group of mice after hypoxia treatment was significantly reduced ( Figure 3 A), while lactate clearance increased significantly ( Figure 3 B). This indicates that under hypoxic conditions, the Lingtao composition can significantly improve the hypoxic physiological adaptability of mice.

[0066] Example 6

[0067] Experiment on the gripping strength of forelimb muscles of Lingtao composite

[0068] 1. Experimental materials:

[0069] 1.1 Lingtao composition: The Lingtao composition prepared in Example 1 was dissolved with physiological saline into low-, medium- and high-dose Lingtao compositions (120 mg / kg body weight, 240 mg / kg body weight, and 360 mg / kg body weight), stored at 4°C after preparation, and preheated to 37°C before each gavage.

[0070] 1.2 Experimental animals: Male SPF-grade C57BL / 6J mice, 8 weeks old, weighing 22 g to 24 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd., with certificate number: 210726240101079667. The breeding conditions were a temperature of 23 ± 2 °C and a relative humidity of 50% ± 10%. During the experiment, the mice had free access to food and water.

[0071] 1.3 Other experimental materials: Tribromoethanol, analytical grade, Shanghai BiDe Pharmaceutical Technology Co., Ltd.

[0072] 2. Experimental methods:

[0073] 28 male C57BL / 6J mice were randomly divided into 4 groups, 7 mice in each group. They were solvent group (normal saline group), low-dose group (120 mg / kg body weight), medium-dose group (240 mg / kg body weight), and high-dose group (360 mg / kg body weight). Different drug treatments were continuously gavaged for 28 days, once a day. For each mouse, the tensile force value was measured by a force sensor equipped with a metal rod (2 mm in diameter and 7.5 cm in length). The maximum force (grams) was used as an indicator of absolute grip strength and was calibrated with the mouse weight.

[0074] 3. Experimental results:

[0075] Compared with the saline group, each dose group could significantly increase the forelimb grasping force of mice. Figure 4 , indicating that the Lingtao combination has a strong effect in increasing muscle strength.

[0076] Example 7

[0077] Experiment on exercise metabolism of Lingtao combination

[0078] 1. Experimental materials:

[0079] 1.1 Lingtao composition: The Lingtao composition prepared in Example 1 was dissolved in physiological saline into low, medium and high doses (120 mg / kg body weight, 240 mg / kg body weight, 360 mg / kg body weight), stored at 4°C after preparation, and preheated to 37°C before each gavage.

[0080] 1.2 Experimental animals: Male SPF-grade C57BL / 6J mice, 8 weeks old, weighing 22 g to 24 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd., with certificate number: 210726240101079667. The breeding conditions were a temperature of 23 ± 2 °C and a relative humidity of 50% ± 10%. During the experiment, the mice had free access to food and water.

[0081] 1.3 Other experimental materials: Tribromoethanol, analytical grade, Shanghai BiDe Pharmaceutical Technology Co., Ltd.

[0082] 2. Experimental methods:

[0083] Twenty-eight male C57BL / 6J mice were randomly divided into four groups, with seven mice in each group. They were respectively a normal saline group, a low-dose Lingtao composition group (120 mg / kg body weight), a medium-dose Lingtao composition group (240 mg / kg body weight), and a high-dose Lingtao composition group (360 mg / kg body weight). Different drug treatments were continuously gavaged for 28 days, once a day. The Oxymax-CLAMS experimental animal detection system (Columbus Instrument, Columbus, USA) was used to detect the oxygen consumption (VO2), carbon dioxide emission (VCO2), heat production (Heat), and respiratory entropy (RER) of mice during autonomous running wheel aerobic endurance exercise.

[0084] 3. Experimental results:

[0085] Figure 5 A: oxygen consumption curve of mice during 30-minute treadmill exercise; B: mean oxygen consumption of mice during 30-minute treadmill exercise; C: carbon dioxide emission curve of mice during 30-minute treadmill exercise; D: mean carbon dioxide emission of mice during 30-minute treadmill exercise; E: respiratory entropy curve of mice during 30-minute treadmill exercise; F: mean respiratory entropy of mice during 30-minute treadmill exercise. Data are mean ± standard deviation. n = 6. *p < 0.05, **p < 0.001, vs. solvent group.

[0086] Four groups of mice were subjected to a treadmill test for exercise metabolism. The mice underwent a 1-hour 10 m / min aerobic running exercise, and the data from the middle 30 minutes were analyzed. The data showed that after the Lingtao composition was treated, the aerobic oxygen consumption of the mice increased ( Figure 5 A and B), CO2 emissions decreased ( Figure 5 C and D), and respiratory entropy decreased significantly ( Figure 5 E and F). The increase in oxygen consumption indicates that the cardiopulmonary function of mice has improved, the decrease in carbon dioxide emission indicates that the oxygen utilization efficiency has improved, and the decrease in respiratory entropy can be regarded as a positive indicator of aerobic exercise training. The above results show that the Lingtao composition can increase the metabolic level and exercise adaptability of mice, reduce fatigue and improve exercise endurance and exercise capacity at the same time.

[0087] Experiments have shown that the experimental results of the anti-fatigue experiment (exhaustive swimming, exhaustive running and ten-minute swimming experiment); anti-hypoxia experiment (hypoxia chamber experiment); forelimb muscle grip experiment; and exercise metabolism experiment of the Lingtao composition prepared in Examples 2 and 3 of the present invention are similar to those of the Lingtao composition prepared in Example 1.

[0088] In summary, the Lingtao composition of the present invention is prepared into corresponding medicines or health foods according to conventional technical means and with pharmaceutically or health food acceptable auxiliary materials, which have the effects of anti-fatigue, prolonging exhaustive exercise time, improving anti-hypoxia physiological adaptability, strengthening muscle strength and enhancing exercise metabolic capacity, and can play a good role in anti-fatigue, anti-hypoxia and improving exercise ability.

[0089] The above is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent change made to the above embodiment according to the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. The use of a Lingtao composition in the preparation of a product for resisting fatigue, resisting hypoxia and improving exercise performance, characterized in that The Lingtao composition is prepared by the following method: 1) taking Smilax glabra and Prunus mume as raw materials in a mass ratio of 1:(0.8-1), adding purified water equivalent to 7-12 times the mass of the raw materials, soaking for 2-3 hours, heating to boil, condensing and refluxing for 1.5-2 hours, filtering, and obtaining an extract a and a residue a; 2) adding purified water equivalent to 4-6 times the mass of the residue a to the residue a, heating and boiling, condensing and refluxing for 1.5-2h, filtering to obtain an extract b and a residue b; combining the extract a and the extract b, concentrating, and freeze-drying to obtain a Lingtao composition.

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