A human decidual natural killer cell separation and sampling device

Abstract

The invention discloses a human decidual natural killer cell separation and sampling device, which relates to the technical field of immune cell sampling; the device comprises a sampling part, a synchronous traction part is installed on the sampling part, a thin film anti-fouling part is installed on the sampling part, and a synchronous traction part is installed inside the thin film anti-fouling part; the synchronous traction part is used to pull the thin film anti-fouling part; a disinfection scraping part is fixedly installed on the sampling part; the disinfection scraping part is used to disinfect the thin film anti-fouling part; the disinfection scraping part is used to disinfect the thin film anti-fouling part; the structure can clean the outer surface of the thin film tube by using the disinfection scraping part in combination with a movable thin film tube, and can play a role of disinfection and sterilization; the problem that the current human decidual natural killer cell separation and sampling device is not convenient for positioning the clear liquid layering position and is prone to misabsorption is solved.

Description

Technical Field

[0001] The invention relates to the technical field of immune cell sampling, in particular to a human decidual natural killer cell separation and sampling device. Background Art

[0002] Decidual natural killer cells are important decidual immune cells. In order to obtain highly pure human decidual natural killer cells (dNK cells), magnetic bead sorting technology can be used. Generally, mononuclear cells are separated from decidual tissue, and then non-CD56+CD16- cells are labeled with a biotin-labeled antibody mixture. The above cells are then labeled with microbeads, washed again, and unlabeled target cells are sorted out by magnetic columns. The cells obtained from the first sorting are labeled with CD56 microbeads, incubated at 4°C, and then washed. Cells labeled with CD56+CD16- are positively selected, and these target cells are collected. The sorted cells are tested by flow cytometry, and then cultured and observed. If the cells are in good condition and aggregated into clusters, they are centrifuged. Centrifugation is performed to collect the clear liquid on the top and the red blood cell sediment at the bottom for sampling. The supernatant is used for subsequent cytokine concentration determination. At present, a suction sampler is usually used for sampling, and the clear liquid needs to be sampled during the process. The human decidual natural killer cell separation sampling device is not convenient for automatic sampling and disinfection. There is a risk of contamination when sampling multiple times. In the later stage of clear liquid extraction, the clear liquid gradually decreases, and manual operation is unstable, which can easily aspirate the deposited red blood cells at the bottom, affecting the quality of clear liquid sampling. It is not convenient to locate the clear liquid stratification point, which is prone to misaspiration. It is also not convenient to quickly switch between clear liquid sampling and deposited red blood cell sampling, which can easily cause the clear liquid sampling and the deposited red blood cells to mix, affecting the accuracy of subsequent suspension preparation.

[0003] To this end, we propose a human decidual natural killer cell isolation and sampling device. Summary of the invention

[0004] The object of the present invention is to provide a human decidual natural killer cell separation and sampling device to solve the problem that the current human decidual natural killer cell separation and sampling device mentioned in the above background technology is not convenient for positioning the clear liquid layer and is prone to misaspiration.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a human decidual natural killer cell separation and sampling device, comprising a sampling part, a synchronous traction part is installed on the sampling part, a thin film anti-fouling part is installed on the sampling part, and a synchronous traction part is installed on the inner side of the thin film anti-fouling part; the synchronous traction part is used to pull the thin film anti-fouling part; a disinfection scraping part is fixedly installed on the sampling part; the disinfection scraping part is used to disinfect the thin film anti-fouling part; a layered positioning part is sleeved on the sampling part, and the layered positioning part is used to isolate the clear liquid after the decidual immune cells are separated; an expansion control part is installed on the layered positioning part, and the expansion control part is used to control the expansion of the layered positioning part; a red blood cell sedimentation sampling part is installed on the layered positioning part; the red blood cell sedimentation sampling part is used to close the layered positioning part; the sampling part comprises: a sampling syringe and a sampling tube, the sampling tube is fixedly installed on the end of the sampling syringe, and the end of the sampling tube is a smooth structure.

[0006] Preferably, the red blood cell sedimentation sampling piece includes: a threaded connection column and a handle, the threaded connection column is threadedly connected in a threaded hole provided on the layered positioning column; the bottom of the threaded connection column is closed and fits the layered positioning column; and a handle is fixedly mounted on the threaded connection column.

[0007] Preferably, the sampling part also includes: a piston, a piston rod and a sampling tension spring, the piston is slidably mounted inside the sampling syringe; the piston is fixedly mounted with a piston rod, and the piston rod is slidably mounted on the sampling syringe; a rubber ring is provided on the outside of the piston; the sampling tension spring is mounted inside the sampling syringe; one end of the sampling tension spring is fixedly mounted on the piston; the other end of the sampling tension spring is fixedly mounted on the inside of the sampling syringe; the sampling tension spring is used to pull the piston to draw in samples.

[0008] Preferably, the film anti-fouling component includes: a film tube, a traction adapter ring and a traction spring, the end of the film tube is fixedly installed on the outside of the connecting block; the film tube is sleeved on the inside and outside of the sampling tube; the film tube fits the inside and outside of the sampling tube; a traction adapter ring is fixedly installed on the other end of the film tube, and the traction adapter ring is sleeved on the outside of the sampling tube; a traction spring is sleeved on the outside of the sampling tube, and the end of the traction spring is connected to the bottom of the sampling tube; the other end of the traction spring is fixedly installed on the traction adapter ring; the connecting block is used to pull the film tube to move.

[0009] Preferably, the red blood cell sedimentation sampling piece also includes: a sponge compression cylinder, which is fixedly sleeved on the outside of the threaded connecting column; the sponge compression cylinder elastically fits in the threaded hole provided on the layered positioning column; the sponge compression cylinder is used to wipe the immune cell clear liquid attached to the clear liquid retaining ring.

[0010] Preferably, the synchronous traction member comprises: a synchronous connecting rod and a connecting block, the synchronous connecting rod is fixedly mounted with the connecting block; the connecting block is provided with a circle of through holes; the end of the synchronous connecting rod is fixedly mounted on the bottom of the piston.

[0011] Preferably, the expansion control component includes: an expansion connecting tube and a control airbag, the expansion connecting tube is fixedly installed on the top of the expansion airbag; a valve is provided on the expansion connecting tube; a control airbag is fixedly installed on the end of the expansion connecting tube; and the control airbag is used to inflate the expansion airbag.

[0012] Preferably, the layered positioning member also includes: a clear liquid retaining ring, an expansion airbag and a support ring, the clear liquid retaining ring is fixedly mounted on the layered positioning column; the layered positioning column is fixedly mounted with an expansion airbag; the expansion airbag is used to be inflated and fit the inner wall of the decidual immune cell separation test tube; a support ring is fixedly mounted on the bottom of the expansion airbag; the support ring is provided with a circle of through holes; the support ring is used to limit the distance between the layered positioning column and the inner wall of the decidual immune cell separation test tube; the layered positioning column is provided with a threaded hole, and the threaded hole on the layered positioning column is concentric with the clear liquid retaining ring.

[0013] Preferably, the disinfection scraping member includes: a disinfection scraping shell, a disinfection sponge ring, a cleaning sponge ring and a rubber scraping ring; the outer side of the sampling tube is fixedly sleeved with a disinfection scraping shell; the inner side of the disinfection scraping shell is fixedly sleeved with a disinfection sponge ring; the inner side of the disinfection sponge ring is attached to the film tube; the side of the disinfection scraping shell is provided with a through hole; the through hole on the side of the disinfection scraping shell is used for injecting disinfectant; the inner side of the disinfection scraping shell is fixedly installed with a cleaning sponge ring, and the cleaning sponge ring is used to fit and wipe the film tube; the inner side of the disinfection scraping shell is fixedly installed with a rubber scraping ring; the inner side of the rubber scraping ring is used to fit and scrape the film tube; the cleaning sponge ring is used to disinfect the film tube.

[0014] Preferably, the layered positioning component includes: a layered positioning column and a docking suction tube, the layered positioning column is used to insert the decidual immune cell separation test tube; a docking suction tube is fixedly installed on the top of the layered positioning column, and a circle of through holes is provided at the bottom of the docking suction tube; the docking suction tube is used to dock a sampling tube; the outer diameter of the layered positioning column is smaller than the inner diameter of the decidual immune cell separation test tube.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The present invention adopts a disinfecting scraper and cooperates with a movable film tube to clean the outer surface of the film tube, which can play a role in disinfection and sterilization. The disinfection is more comprehensive and avoids the problem that the end of the sampling tube of the traditional sampling structure is easily contaminated. Disinfection can be automatically performed each time sampling is taken to prevent the end of the sampling tube from being contaminated with bacteria when the staff performs sampling work; it has no sense of operation and a better user experience.

[0017] The expansion control part can be used to control the expansion of the inflation balloon to fit the inner wall of the decidual immune cell separation tube for positioning, which is stable and convenient. The layered positioning part can be used multiple times after disinfection and cleaning. The layered positioning part can facilitate the staff to locate and separate the deposited red blood cells and the clear liquid in the decidual immune cell separation tube, avoiding the problem of accidental inhalation of deposited red blood cells when the clear liquid level is not high in the later stage of clear liquid extraction. If the clear liquid is retained, it is easy to cause the problem of clear liquid waste. The use of stably expanding layered positioning parts can also prevent the doctor's hand shaking when extracting the clear liquid, which may cause the deposited red blood cells at the bottom to mix with the clear liquid and become turbid, which can reduce the difficulty of sampling operation for doctors, with high sampling accuracy and purer clear liquid.

[0018] The use of the red blood cell sedimentation sampling piece can make it easier and faster to sample the deposited red blood cells, can prevent the entry of clear liquid when sampling the deposited red blood cells, can ensure the purity of the deposited red blood cells, can be quickly switched, is more convenient in the subsequent preparation of the red blood cell suspension, and reduces the interference of the clear liquid on the sampling of the deposited red blood cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the overall structure of a human decidual natural killer cell separation and sampling device of the present invention;

[0020] Figure 2 This is a cross-sectional view of the internal structure of a human decidual natural killer cell separation and sampling device of the present invention;

[0021] Figure 3 This is a schematic diagram of the bottom structure of a human decidual natural killer cell separation and sampling device of the present invention;

[0022] Figure 4 It is a schematic diagram of the structure of the sampling part of the present invention;

[0023] Figure 5 For the present invention Figure 4 A magnified view of the structure of the middle B area;

[0024] Figure 6 This is a schematic diagram of the structure of the thin film antifouling member of the present invention;

[0025] Figure 7 This is a schematic diagram of the installation position of the film cylinder of the present invention;

[0026] Figure 8 For the present invention Figure 7 A magnified view of the structure of the middle D region;

[0027] Fig. 9 This is a schematic diagram of the structure of the layered positioning column of the present invention;

[0028] Fig.10This is a schematic diagram of the installation position of the layered positioning column of the present invention in the decidual immune cell test tube;

[0029] Fig.11 For the present invention Figure 2 A magnified view of the structure of the middle F region;

[0030] Fig.12 This is a schematic diagram of the installation position of the disinfection scraper of the present invention.

[0031] In the figure: 1. sampling part; 101. sampling syringe; 102. sampling tube; 103. piston; 1031. piston rod; 104. sampling tension spring; 2. synchronous traction member; 201. synchronous connecting rod; 202. connecting block; 3. film antifouling member; 301. film tube; 302. traction adapter ring; 303. traction tension spring; 4. disinfection scraper; 401. disinfection scraper shell; 402. disinfection sponge ring; 403. cleaning sponge ring; 404. rubber scraper ring; 5. layered positioning member; 501. layered positioning column; 5011. docking suction tube; 5012. clear liquid retaining ring; 502. expansion airbag; 503. support ring; 6. expansion control member; 601. expansion connecting tube; 602. control airbag; 7. red blood cell sedimentation sampling member; 701. threaded connecting column; 7011. handle; 702. sponge compression cylinder. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Embodiment 1

[0034] See also Figure 1-Figure 12 As shown:

[0035] The present invention provides a technical solution: a human decidual natural killer cell separation sampling device, comprising a sampling part 1, a synchronous traction part 2 is installed on the sampling part 1, a thin film anti-fouling part 3 is installed on the sampling part 1, and the synchronous traction part 2 is installed on the inner side of the thin film anti-fouling part 3; the synchronous traction part 2 is used to pull the thin film anti-fouling part 3; a disinfection scraping part 4 is fixedly installed on the sampling part 1; the disinfection scraping part 4 is used to disinfect the thin film anti-fouling part 3; a layered positioning part 5 is sleeved on the sampling part 1, and the layered positioning part 5 is used to isolate the clear liquid after the decidual immune cells are separated; an expansion control part 6 is installed on the layered positioning part 5, and the expansion control part 6 is used to control the expansion of the layered positioning part 5; a red blood cell sediment sampling part 7 is installed on the layered positioning part 5; the red blood cell sediment sampling part 7 is used to close the layered positioning part 5; the sampling part 1 comprises: a sampling syringe 101 and a sampling tube 102, the sampling syringe 101 end is fixedly installed with the sampling tube 102, and the end of the sampling tube 102 is a smooth structure.

[0036] The sampling part 1 also includes: a piston 103, a piston rod 1031 and a sampling tension spring 104, the piston 103 is slidably sleeved inside the sampling syringe 101; a piston rod 1031 is fixedly installed on the piston 103, and the piston rod 1031 is slidably installed on the sampling syringe 101; a rubber ring is provided on the outside of the piston 103; a sampling tension spring 104 is sleeved inside the sampling syringe 101; the end of the sampling tension spring 104 is fixedly installed on the piston 103; the other end of the sampling tension spring 104 is fixedly installed on the inner side of the sampling syringe 101; the sampling tension spring 104 is used to pull the piston 103 to extract the sample; the synchronous traction part 2 includes: a synchronous connecting rod 201 and a connecting block 202, the synchronous connecting rod 201 is fixedly installed with a connecting block 202; a circle of through holes is provided on the connecting block 202; the end of the synchronous connecting rod 201 is fixedly installed at the bottom of the piston 103; the film anti-fouling part 3 includes: a film cylinder 3 01. A traction adapter ring 302 and a traction spring 303. The end of the film tube 301 is fixedly mounted on the outside of the connecting block 202; the film tube 301 is sleeved on the inner and outer sides of the sampling tube 102; the film tube 301 fits the inner and outer sides of the sampling tube 102; a traction adapter ring 302 is fixedly mounted on the other end of the film tube 301, and the traction adapter ring 302 is sleeved on the outside of the sampling tube 102; a traction spring 303 is sleeved on the outside of the sampling tube 102, and the end of the traction spring 303 is connected to the bottom of the sampling tube 102; the other end of the traction spring 303 is fixedly mounted on the traction adapter ring 302; the connecting block 202 is used to traction the film tube 301 to move, and the sampling part 1 can be used to facilitate quick sampling. At the same time, the movable film anti-fouling part 3 is used to facilitate subsequent disinfection and cleaning work. The sampling spring 104 is used to pull the piston 103 to draw the sample, which is simple for doctors to operate and easy to use.

[0037] The disinfection scraper 4 includes: a disinfection scraper shell 401, a disinfection sponge ring 402, a cleaning sponge ring 403 and a rubber scraper ring 404. The outer side of the sampling tube 102 is fixedly sleeved with the disinfection scraper shell 401; the inner side of the disinfection scraper shell 401 is fixedly sleeved with the disinfection sponge ring 402; the inner side of the disinfection sponge ring 402 is attached to the film tube 301; a through hole is provided on the side of the disinfection scraper shell 401; the through hole on the side of the disinfection scraper shell 401 is used to put in disinfectant; the disinfection scraper shell A cleaning sponge ring 403 is fixedly installed on the inner side of 401, and the cleaning sponge ring 403 is used to fit and wipe the film tube 301; a rubber scraping ring 404 is fixedly installed on the inner side of the disinfection scraping shell 401; the inner side of the rubber scraping ring 404 is used to fit and scrape the film tube 301; the cleaning sponge ring 403 is used to disinfect the film tube 301, and the disinfection scraping member 4 is used to cooperate with the movable film tube 301. This structure can clean the outer surface of the film tube 301, which can The structure can be disinfected and sterilized each time, and the end of the sampling tube 102 of the traditional sampling structure can be prevented from being contaminated by bacteria when the staff performs sampling work. The movable film tube 301 can be used for self-cleaning. At the same time, the cleaning sponge ring 403 and the rubber scraper ring 404 can also keep the surface of the film tube 301 wiping to prevent residual disinfectant on the surface, and the cleaning effect is better. The structure can achieve cleaning when the piston rod 1031 is pressed, and the operation is seamless, and the user experience is better. When the film tube 301 moves, the disinfection sponge ring 402 can be used for wiping. The film tube 301 attached to the inner side of the disinfection sponge ring 402 will move up when the piston rod 1031 is pressed. At this time, the film tube 301 located at the end of the sampling tube 102 will gradually be driven to the inner side of the disinfection sponge ring 402 for disinfection and wiping.

[0038] The layered positioning member 5 includes: a layered positioning column 501 and a docking suction tube 5011, the layered positioning column 501 is used to insert the decidual immune cell separation test tube; the layered positioning column 501 is fixedly installed with a docking suction tube 5011 on the top, and a circle of through holes is provided at the bottom of the docking suction tube 5011; the docking suction tube 5011 is used to dock the sampling tube 102; the outer diameter of the layered positioning column 501 is smaller than the inner diameter of the decidual immune cell separation test tube; the layered positioning member 5 also includes: a clear liquid retaining ring 5012, an expansion airbag 502 and a support ring 503, the clear liquid retaining ring 5012 is fixedly installed on the layered positioning column 501; the layered positioning column 501 is fixedly installed with an expansion airbag 502; the expansion airbag 502 is fixedly installed on the layered positioning column 501 ... The inflation bag 502 is used to inflate and fit the inner wall of the decidual immune cell separation test tube; a support ring 503 is fixedly installed at the bottom of the inflation bag 502; a circle of through holes is provided on the support ring 503; the support ring 503 is used to limit the distance between the layered positioning column 501 and the inner wall of the decidual immune cell separation test tube; a threaded hole is provided on the layered positioning column 501, and the threaded hole on the layered positioning column 501 is concentric with the clear liquid retaining ring 5012; the inflation control member 6 includes: an inflation connecting tube 601 and a control bag 602, the inflation connecting tube 601 is fixedly installed on the top of the inflation bag 502; a valve is provided on the inflation connecting tube 601; a control bag 60 is fixedly installed at the end of the inflation connecting tube 601 2; the control airbag 602 is used to inflate the expansion airbag 502; the expansion control member 6 can be used to control the expansion airbag 502 to expand and fit the inner wall of the decidual immune cell separation test tube for positioning, which is stable and convenient. The layered positioning member 5 can be used to facilitate the staff to locate and separate the deposited red blood cells and the clear liquid in the decidual immune cell separation test tube, so as to avoid the problem that the clear liquid level is not high in the late stage of clear liquid extraction, and the deposited red blood cells are easily inhaled by mistake. If the clear liquid is retained, it is easy to cause waste of the clear liquid and increase the clear liquid separation step. The stable expansion layered positioning member 5 can also prevent the doctor's hand shaking when extracting the clear liquid, which causes the red blood cells deposited at the bottom to mix with the clear liquid and become turbid. The layered positioning member 5 used The positioning column 501 is easy to use. The layered positioning column 501 is inserted into the decidual immune cell separation test tube, and the handle 7011 is slowly moved downward. At this time, the upper clear liquid will not be stirred. When the layered positioning column 501 moves down to the boundary between the deposited red blood cells and the clear liquid, the color difference between the deposited red blood cells and the clear liquid can be used to quickly perform the isolation operation; the outer side of the clear liquid retaining ring 5012 is used to stop the clear liquid remaining on the top of the layered positioning column 501, and the support ring 503 is used to play a supporting role when the layered positioning column 501 is inserted into the decidual immune cell separation test tube, thereby improving the stability of the layered positioning column 501 after the expansion balloon 502 is expanded, and the through hole is set on the support ring 503 so as not to affect the liquid circulation.

[0039] Embodiment 2

[0040] On the basis of the first embodiment, the red blood cell sedimentation sampling piece 7 comprises: a threaded connection column 701 and a handle 7011, the threaded connection column 701 is threadedly connected in the threaded hole provided on the layered positioning column 501; the bottom of the threaded connection column 701 is closed and fits the layered positioning column 501; the handle 7011 is fixedly installed on the threaded connection column 701; the red blood cell sedimentation sampling piece 7 also comprises: a sponge compression cylinder 702, the sponge compression cylinder 702 is fixedly sleeved on the outside of the threaded connection column 701; the sponge compression cylinder 702 is elastically fitted in the threaded hole provided on the layered positioning column 501; the sponge compression cylinder 702 is used to wipe the free attached to the clear liquid retaining ring 5012 The immune cell clear liquid adopts the red blood cell sedimentation sampling piece 7, which can be more convenient for quickly sampling the deposited red blood cells, can prevent the clear liquid from entering when sampling the deposited red blood cells, can ensure the purity of the deposited red blood cells, can be quickly switched, and is more convenient for the subsequent red blood cell suspension preparation work, and reduces the interference of the clear liquid on the sampling of the deposited red blood cells. The handheld handle 7011 is rotated to rotate the threaded connecting column 701. At this time, the layered positioning column 501 can drive the sponge compression cylinder 702 to be taken out together. When the sponge compression cylinder 702 is taken out, it will elastically adhere to the outside of the clear liquid retaining ring 5012 for wiping, and the clear liquid remaining on the clear liquid retaining ring 5012 is wiped off.

[0041] The working principle of this embodiment is as follows: first, the hand-held handle 7011 is slowly moved downward, at which time the upper clear liquid will not be stirred up. When the stratified positioning column 501 moves down to the boundary between the deposited red blood cells and the clear liquid, the doctor observes that the deposited red blood cells are about to approach the lower edge of the expansion balloon 502 by using the color difference between the deposited red blood cells and the clear liquid. The doctor can press the control balloon 602 to control the expansion balloon 502 to expand and position, and close the valve on the expansion connecting tube 601. At this time, the standard clear liquid is located above the stratified positioning column 501, and sampling and suction can be performed quickly; by holding the sampling syringe 101 and pressing down the piston 103, the piston rod 1031 is released at this time, and the sampling tension spring 104 will pull the piston 103 to draw the sample. When the piston 103 moves downward, it drives the connecting block 202 to move downward together, and cooperates with the pulling of the traction tension spring 303 to drive the traction adapter ring 302 to move upward, and control the film cylinder 301 to move in close contact with the inside and outside of the sampling tube 102. Similarly, when the piston is released, When the piston rod 103 is pressed, the sampling tension spring 104 will pull the piston 103 upward, and also drive the film tube 301 to move and reset, and the clear liquid will be discharged through the piston 103; when the film tube 301 moves, the disinfection sponge ring 402 can be used for wiping, and the film tube 301 attached to the inner side of the disinfection sponge ring 402 will move up when the piston rod 1031 is pressed. At this time, the film tube 301 at the end of the sampling tube 102 will be gradually driven to the inner side of the disinfection sponge ring 402 After disinfection and wiping, when the piston rod 1031 is released, the film tube 301 is reset. At this time, the outer surface of the film tube 301 is wiped by the cleaning sponge ring 403 and is fitted with the rubber scraper ring 404 to remove water, so as to keep the surface clean. When sampling, the front end of the sampling tube 102 can be used to contact the clear liquid for sampling. When there is less residual clear liquid, the sampling tube 102 can be docked with the docking suction tube 5011, and the uniform through holes on the outside of the docking suction tube 5011 can be used to assist in absorbing the residual clear liquid.

[0042] By holding the handle 7011 and rotating the threaded connecting column 701, the handle 7011 can be driven to rotate and separate from the stratified positioning column 501. At this time, the stratified positioning column 501 can drive the sponge compression cylinder 702 to be taken out together. When the sponge compression cylinder 702 is taken out, it will elastically adhere to the outside of the clear liquid retaining ring 5012 for wiping, and the clear liquid remaining on the clear liquid retaining ring 5012 can be wiped off. At this time, sampling can be carried out by using a new sampling part 1. When sampling the deposited red blood cells, if it is necessary to sample the deposited red blood cells multiple times, after each quantitative sampling, the stratified positioning column 501 can be appropriately lowered for a certain distance to keep the small amount of clear liquid between the outside of the stratified positioning column 501 and the inner wall of the decidual immune cell separation test tube always isolated to ensure the sampling quality.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0044] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A human decidual natural killer cell separation and sampling device, comprising a sampling portion (1), wherein a synchronous traction member (2) is installed on the sampling portion (1), characterized in that: The sampling portion (1) is provided with a thin film anti-fouling component (3), and a synchronous traction component (2) is provided inside the thin film anti-fouling component (3); the synchronous traction component (2) is used to traction the thin film anti-fouling component (3); The sampling portion (1) is provided with a disinfecting scraping member (4); the disinfecting scraping member (4) is used to disinfect the thin film anti-fouling member (3); The sampling portion (1) is sleeved with a layered positioning member (5), and the layered positioning member (5) is used to isolate the clear fluid after the decidual immune cells are separated; An expansion control member (6) is installed on the layered positioning member (5), and the expansion control member (6) is used to control the expansion of the layered positioning member (5); A red blood cell sediment sampling piece (7) is installed on the layered positioning piece (5); the red blood cell sediment sampling piece (7) is used to seal the layered positioning piece (5); The sampling part (1) comprises: a sampling syringe (101) and a sampling tube (102); the sampling tube (102) is fixedly mounted on the end of the sampling syringe (101), and the end of the sampling tube (102) is a smooth structure; The sampling part (1) further comprises: a piston (103), a piston rod (1031) and a sampling tension spring (104); the piston (103) is slidably sleeved inside the sampling syringe (101); the piston (103) is fixedly mounted with the piston rod (1031), and the piston rod (1031) is slidably mounted on the sampling syringe (101); a rubber ring is arranged on the outside of the piston (103); a sampling tension spring (104) is sleeved inside the sampling syringe (101); the end of the sampling tension spring (104) is fixedly mounted on the piston (103); the other end of the sampling tension spring (104) is fixedly mounted on the inside of the sampling syringe (101); the sampling tension spring (104) is used to pull the piston (103) to draw out the sample; The synchronous traction member (2) comprises: a synchronous connecting rod (201) and a connecting block (202); the connecting block (202) is fixedly mounted on the synchronous connecting rod (201); a circle of through holes is provided on the connecting block (202); the end of the synchronous connecting rod (201) is fixedly mounted on the bottom of the piston (103); The thin film anti-fouling component (3) comprises: a thin film tube (301), a traction adapter ring (302) and a traction tension spring (303); the end of the thin film tube (301) is fixedly mounted on the outside of the connecting block (202); the thin film tube (301) is sleeved on both sides of the inside and outside of the sampling tube (102); the thin film tube (301) is fitted with both sides of the inside and outside of the sampling tube (102); the other end of the thin film tube (301) is fixedly mounted with a traction adapter ring (302), and the traction adapter ring (302) is sleeved on the outside of the sampling tube (102); the outside of the sampling tube (102) is sleeved with a traction tension spring (303), and the end of the traction tension spring (303) is connected to the bottom of the sampling tube (102); the other end of the traction tension spring (303) is fixedly mounted on the traction adapter ring (302); the connecting block (202) is used to traction the thin film tube (301) to move; The disinfection scraping member (4) comprises: a disinfection scraping shell (401), a disinfection sponge ring (402), a cleaning sponge ring (403) and a rubber scraping ring (404); the outer side of the sampling tube (102) is fixedly sleeved with the disinfection scraping shell (401); the inner side of the disinfection scraping shell (401) is fixedly sleeved with the disinfection sponge ring (402); the inner side of the disinfection sponge ring (402) is attached to the film tube (301); the side of the disinfection scraping shell (401) is provided with a through hole; the disinfection The through hole on the side of the disinfectant scraping shell (401) is used for injecting disinfectant; a cleaning sponge ring (403) is fixedly installed on the inner side of the disinfectant scraping shell (401), and the cleaning sponge ring (403) is used to fit the wiping film tube (301); a rubber scraping ring (404) is fixedly installed on the inner side of the disinfectant scraping shell (401); the inner side of the rubber scraping ring (404) is used to fit the scraping film tube (301); the cleaning sponge ring (403) is used to disinfect the film tube (301); The layered positioning member (5) comprises: a layered positioning column (501) and a docking suction tube (5011), wherein the layered positioning column (501) is used to be inserted into a decidual immune cell separation test tube; a docking suction tube (5011) is fixedly mounted on the top of the layered positioning column (501), and a circle of through holes is provided at the bottom of the docking suction tube (5011); the docking suction tube (5011) is used to dock with a sampling tube (102); and the outer diameter of the layered positioning column (501) is smaller than the inner diameter of the decidual immune cell separation test tube.

2. The human decidual natural killer cell separation and sampling device according to claim 1, characterized in that: The layered positioning member (5) further comprises: a clear liquid retaining ring (5012), an expansion airbag (502) and a support ring (503); the clear liquid retaining ring (5012) is fixedly mounted on the layered positioning column (501); the expansion airbag (502) is fixedly mounted on the layered positioning column (501); the expansion airbag (502) is used to be inflated and fit the inner wall of the decidual immune cell separation test tube; a support ring (503) is fixedly mounted on the bottom of the expansion airbag (502); a circle of through holes is provided on the support ring (503); the support ring (503) is used to limit the distance between the layered positioning column (501) and the inner wall of the decidual immune cell separation test tube; the layered positioning column (501) is provided with a threaded hole, and the threaded hole on the layered positioning column (501) is concentric with the clear liquid retaining ring (5012).

3. A human decidual natural killer cell separation and sampling device according to claim 2, characterized in that: The expansion control component (6) comprises: an expansion connecting tube (601) and a control airbag (602); the expansion connecting tube (601) is fixedly mounted on the top of the expansion airbag (502); a valve is provided on the expansion connecting tube (601); a control airbag (602) is fixedly mounted on the end of the expansion connecting tube (601); and the control airbag (602) is used to inflate the expansion airbag (502).

4. The human decidual natural killer cell separation and sampling device according to claim 2, characterized in that: The red blood cell sedimentation sampling piece (7) comprises: a threaded connection column (701) and a handle (7011); the threaded connection column (701) is threadedly connected in a threaded hole provided on the layered positioning column (501); the bottom of the threaded connection column (701) is closed and fits the layered positioning column (501); and the handle (7011) is fixedly mounted on the threaded connection column (701).

5. The human decidual natural killer cell separation and sampling device according to claim 4, characterized in that: The red blood cell sedimentation sampling piece (7) further comprises: a sponge compression cylinder (702), wherein the sponge compression cylinder (702) is fixedly sleeved on the outside of the threaded connection column (701); the sponge compression cylinder (702) is elastically fitted into a threaded hole provided on the layered positioning column (501); and the sponge compression cylinder (702) is used to wipe the immune cell clear liquid attached to the clear liquid retaining ring (5012).

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