Cell traction force loading device
By designing a dual-slide structure driven by laser ranging sensor and servo motor, the problem that the cell tension loading device in the prior art cannot achieve high resolution observation, and high resolution observation of internal changes of cells under high-power microscopes is achieved and stable stretching effect.
Patent Information
- Application Number
- CN202411261264.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-10
- Publication Date
- 2024-12-13
AI Technical Summary
The existing cell tension loading device cannot achieve high-resolution dynamic monitoring of cells in the stressed state and real-time observation of internal organelles changes, especially under high-power microscopes.
A cell tension loading device including a frame part, a sliding part and a clamping part is designed, and a laser ranging sensor and a servo motor are used for precise stretching. Combined with a tension sensor and a dual-slide structure, it ensures stable stretching of the biofilm. and high-precision measurement, while using anti-rust materials and comb baffles to prevent leakage of culture fluid.
High resolution observation of cells under a high-power microscope under a stressed state is achieved, ensuring the stability and accuracy of the cell stretching process. The device is small and flat, and does not require a whole soak in the cell fluid.
Smart Images
Figure CN119120185A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of cell tension loading devices, in particular to a tension loading device which can cooperate with a microscope to perform high-resolution observation of the change process of cells and their internal organelles. Background Art
[0002] Distraction osteogenesis is based on the biological characteristics of bone tissue - bone tissue can stimulate new bone formation under slow and continuous distraction. However, the changes in tissue cells under distraction are still not very clear. Limited by the inability to directly observe and study in vivo, in vitro devices are currently mainly used to apply force to cells to observe cell changes. The specific cell force devices are mainly of the following types:
[0003] (1) Mainly by controlling the vacuum pump (vacuum pump and storage-variable gas pressure system) connected to the vacuum chamber, the negative pressure in the vacuum chamber changes periodically, so that the elastic basement membrane of the culture plate sealed on it is deformed, so that the cells growing on the wall are mechanically stretched. However, this force-adding method causes the cells to be subjected to forces in multiple directions, which is different from the horizontal force generated by stretching. At the same time, because it is connected to the vacuum chamber, it is impossible to achieve real-time high-resolution observation of the cell changes during the force loading process;
[0004] (2) Using polydimethylsiloxane membrane (PDMS) and high-precision stepper motors to achieve cell tensile loading. This force loading mode is similar to tensile force, but the stretching device and PDMS membrane are integrated into the host, and cells can only be observed at low magnification (~10×) before and after stretching, and high-resolution dynamic monitoring of cell changes under force cannot be achieved;
[0005] (3) Uniaxial forces similar to stretching can be applied, but a sterile disposable silicone plate composed of a thin transparent film with a thickness greater than 0.17 mm is required, which makes it impossible to achieve high-resolution observation with an oil immersion lens of more than 60 times;
[0006] (4) It integrates a cell mechanical loading instrument test system and an environmental control system, and can culture bones, cartilage, or any other disc-shaped samples under force, but cannot achieve force-added culture of cells;
[0007] (5) Cell stretch culture system, which can provide periodic stretching force to cells. However, since the cell culture chamber and the force-adding device are integrated in a closed space, real-time observation of the cell force-adding process cannot be achieved.
[0008] It can be seen that the existing devices have obvious deficiencies in the force application mode and real-time high-resolution observation of cell changes under the force application state. There is an urgent need for a distraction force loading device that is consistent with the force application mode of distraction osteogenesis and can cooperate with a high-power microscope to perform high-resolution observation of the change process of cells and their internal organelles. Summary of the invention
[0009] The main purpose of the present invention is to provide a cell tensile force loading device, so as to realize the high-resolution observation of the change process of the internal organelles of the cell under the force-applied state by using a high-power microscope.
[0010] To achieve the above-mentioned purpose, the present invention provides a cell tension loading device, which mainly includes a frame part, a sliding part and a clamping part; the frame part is a hollow frame structure formed by fixing a bottom plate, a cover plate, a front guide rod fixing block and a rear guide rod fixing block by bolts, the front guide rod fixing block has a hole in the middle, and a laser ranging sensor is fixedly arranged on the front side thereof, the rear guide rod fixing block has a hole in the middle, and a motor is fixedly arranged on the rear side thereof; the sliding part includes two guide rods and a slider, the two ends of the guide rods are respectively fixed on the front guide rod fixing block and the rear guide rod fixing block, the slider can slide back and forth on the two guide rods, a tension sensor is fixedly arranged on the rear side of the slider, and the rear end of the tension sensor is connected to the motor telescopic shaft of the motor; the clamping part is used to clamp the biofilm, and its front end is fixed on the lower side of the front part of the bottom plate, and the rear end is fixed on the lower side of the slider.
[0011] Optionally, the front and rear ends of the clamping part are composed of a membrane limiting block and a ball head positioning pin, wherein the front end membrane limiting block is fixed on the bottom plate, and the rear end membrane limiting block is fixed on the slider. The biofilm adopts market standard parts and can be fixed directly using the ball head positioning pin.
[0012] Optionally, comb-shaped baffles are provided on both sides of the middle of the clamping portion to prevent leakage of the culture solution.
[0013] Optionally, the surface of the slider is sandblasted to increase its surface roughness, thereby ensuring that the laser emitted by the laser ranging sensor generates diffuse reflection after irradiating the slider surface, and the reflected light can enter the receiving window of the laser ranging sensor to ensure accurate distance measurement.
[0014] Optionally, the slider and the guide rod are connected to the linear bearing via a retaining spring so as to firmly connect the slider and the linear bearing.
[0015] Optionally, the motor is a servo motor, which can control the stretching distance more accurately.
[0016] Optionally, the motor and the rear guide rod fixing block are connected via a motor fixing block, and the tension sensor and the motor telescopic shaft are connected via a motor transition piece.
[0017] Optionally, the frame portion, the sliding portion and the clamping portion are all made of anti-corrosion materials.
[0018] The cell tensile force loading device proposed in the embodiment of the present invention can be used in conjunction with a high-power microscope to perform high-resolution observation of the changes in the internal organelles of cells under force application. At the same time, the device can be flattened and small in size, and the cell stretching process is more stable, and the entire device does not need to be immersed in cell fluid. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 This is a structural diagram of the cell tension loading device of the present invention;
[0020] Figure 2 This is a structural diagram of the cell tension loading device (back side) of the present invention.
[0021] Labeling: 1-base plate, 2-cover plate, 3-laser distance sensor, 4-front guide rod fixing block, 5-rear guide rod fixing block, 6-motor fixing block, 7-motor telescopic shaft, 8-motor, 9-guide rod, 10-slider, 11-linear bearing, 12-tension sensor, 13-motor transition piece, 14-biofilm, 15-front end limit film block, 16-rear end limit film block, 17-ball head positioning pin, 18-hole, 19-comb baffle, 20-comb baffle, 21-hole DETAILED DESCRIPTION
[0022] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.
[0023] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.
[0024] The cell tension loading device of the embodiment of the present invention mainly includes a frame part, a sliding part and a clamping part. Figure 1 , Figure 2As shown, the frame portion is a hollow frame structure formed by fixing a bottom plate 1, a cover plate 2, a front guide rod fixing block 4 and a rear guide rod fixing block 5 by bolts; a hole 18 is provided in the middle of the front guide rod fixing block 4, a laser ranging sensor 3 is fixedly arranged on the front side of the front guide rod fixing block 4, the laser ranging sensor 3 transmits and receives lasers through the hole 18, and the hole 18 is preferably square; a hole 21 is provided in the middle of the rear guide rod fixing block 5, a motor 8 is fixedly arranged on the rear side, the hole 21 is preferably circular, the motor telescopic shaft 7 of the motor 8 can pass through the hole 21, the motor 8 is preferably a servo motor, so as to more accurately control the stretching distance, and the motor 8 and the rear guide rod fixing block 5 are preferably connected via the motor fixing block 6.
[0025] The sliding part includes two guide rods 9 and a slider 10, the two ends of the two guide rods 9 are respectively fixed on the front guide rod fixing block 4 and the rear guide rod fixing block 5, and the slider 10 can slide back and forth on the two guide rods; optimally, the slider 10 and the guide rod 9 are connected to the linear bearing 11 through a retaining spring so as to firmly connect the slider 10 and the linear bearing 11; at the same time, the surface of the slider 10 is optimally sandblasted to increase its surface roughness, thereby ensuring that the laser emitted by the laser ranging sensor 3 is diffusely reflected after being irradiated to the surface of the slider 10, and the reflected light can enter the receiving window of the laser ranging sensor, so as to measure the distance more accurately; a tension sensor 12 is fixedly arranged on the rear side of the slider 10, and the rear end of the tension sensor 12 is connected to the motor telescopic shaft 7 of the motor 8, and optimally, the tension sensor 12 is connected to the motor telescopic shaft through a motor transition piece 13.
[0026] The clamping part is used to clamp the biofilm 14, the front end of the clamping part is fixed to the lower side of the front part of the base plate 1, and the rear end is fixed to the lower side of the slider 10; optimally, the front and rear ends of the clamping part are composed of membrane limiting blocks 15, 16 and ball head positioning pins 17, wherein the front end membrane limiting block 15 is fixed on the base plate 1, and the rear end membrane limiting block 16 is fixed on the slider 10, and the biofilm 14 adopts market standard parts, and the biofilm 14 can be fixed directly using the ball head positioning pins 17; in addition, comb-shaped baffles 19, 20 are preferably provided on both sides of the middle of the clamping part, wherein the comb-shaped baffle 20 is connected to the front end membrane limiting block 15, and the comb-shaped baffle 19 is connected to the rear end membrane limiting block 16, and the comb-shaped baffle 19 can slide back and forth with the slider 10, and when the slider 10 slides to the maximum position, the comb-shaped baffle 19 and the comb-shaped baffle 20 can also jointly prevent the leakage of the culture solution.
[0027] The frame portion, the sliding portion and the clamping portion are preferably made of anti-corrosion materials.
[0028] When the device of the present invention is used, it can be placed on a customized table to ensure that the slider 10 can slide freely. When the motor 8 rotates, the slider 10 can be driven to move axially with the guide rod 9 as the axis. The double guide rod structure can ensure that the biofilm 14 will not deform. At the same time, the stretching amount and stretching force of the biofilm 14 can be detected at any time through the laser distance sensor 3 and the tension sensor 12. In addition, a high-power microscope used in conjunction can be placed under the biofilm 14 to clearly observe the entire change process of the cells under the stretching state.
[0029] The cell tensile force loading device proposed in the embodiment of the present invention can be used in conjunction with a high-power microscope to perform high-resolution observation of the changes in the internal organelles of cells under a force-applied state. At the same time, the device can be flattened and small in size. Due to the use of a double slide rail structure, the cell stretching process is more stable, and the entire device does not need to be immersed in the cell fluid when observing with a matching high-power microscope.
Claims
1. A cell tension loading device, characterized in that: The device mainly comprises a frame part, a sliding part and a clamping part; the frame part is a hollow frame structure formed by fixing a bottom plate (1), a cover plate (2), a front guide rod fixing block (4) and a rear guide rod fixing block (5) by bolts; the front guide rod fixing block (4) has a hole (18) in the middle, and a laser distance measuring sensor (3) is fixedly arranged on the front side; the rear guide rod fixing block (5) has a hole (21) in the middle, and a motor (8) is fixedly arranged on the rear side; the sliding part comprises two guide rods (9) and a sliding part. The guide rod (9) is fixed to a front guide rod fixing block (4) and a rear guide rod fixing block (5) respectively. The slider (10) can slide forward and backward on the two guide rods (9). A tension sensor (12) is fixedly arranged on the rear side of the slider (10). The rear end of the tension sensor (12) is connected to the motor telescopic shaft (7) of the motor (8). The clamping part is used to clamp the biofilm (14). The front end of the clamping part is fixed to the lower side of the front part of the base plate (1), and the rear end is fixed to the lower side of the slider (10).
2. The cell tension loading device according to claim 1, characterized in that: The front and rear ends of the clamping part are both composed of a film limiting block and a ball head positioning pin (17), wherein the front film limiting block (15) is fixed on the bottom plate (1), and the rear film limiting block (16) is fixed on the slider (10).
3. The cell tension loading device according to claim 2, characterized in that: Comb-shaped baffles are arranged on both sides of the middle of the clamping part, wherein the comb-shaped baffle (20) is connected to the front end film limiting block (15), and the comb-shaped baffle (19) is connected to the rear end film limiting block (16).
4. The cell tension loading device according to claim 1, characterized in that: The surface of the sliding block (10) is sandblasted.
5. The cell tension loading device according to claim 1, characterized in that: The slide block (10) and the guide rod (9) are connected to the linear bearing (11) via a retaining spring.
6. The cell tension loading device according to claim 1, characterized in that: The motor (8) is a servo motor.
7. The cell tension loading device according to claim 1, characterized in that: The motor (8) and the rear guide rod fixing block (5) are connected via a motor fixing block (6).
8. The cell tension loading device according to claim 1, characterized in that: The tension sensor (12) is connected to the motor telescopic shaft (7) via a motor transition piece (13).
9. The cell tension loading device according to claim 3, characterized in that: The frame part, the sliding part and the clamping part are all made of anti-corrosion materials.