Method for determining weak welding sealing performance between cavities of multi-cavity product

Abstract

The invention discloses a method for determining weak welding sealing performance between cavities of a multi-cavity product, and the method for determining the weak welding sealing performance between the cavities of the multi-cavity product comprises the steps that the multi-cavity product is provided, the multi-cavity product at least comprises a first cavity and a second cavity, the first chamber and the second chamber are separated by a weak welding rod, the first chamber is filled with a bacterial suspension, and the second chamber is filled with a microbial culture medium; culturing the multi-chamber product at a preset temperature for a preset time; and observing whether the cultured culture medium in the second chamber is turbid or not so as to determine the sealing property of the multi-chamber product. The method provided by the invention has higher sensitivity, can detect leak holes with smaller apertures in the sealing strip, and is more suitable for weak welding sealing performance detection among cavities of a multi-cavity product. In addition, the method can be used for guiding the parameter development of the weak welding sealing process and verifying the weak welding sealing process.

Description

Technical Field

[0001] The present invention belongs to the field of packaging products, and more particularly, the present invention generally relates to a method for determining the sealing performance of weak welds between chambers of a multi-chamber product, and a method for producing a multi-chamber product by means of the same. Background Art

[0002] In the pharmaceutical industry, in order to achieve combined medication, synergistic medication, or solve product stability issues, some products gradually adopt multi-chamber designs. In addition, in the food and cosmetics industries, with the development of new packaging, multi-chamber design products have also begun to enter the public eye.

[0003] The chambers of multi-chamber products are generally sealed by weak welding to ensure that the contents in the multiple chambers are independent and not mixed during storage. When in use, they can be opened and mixed by applying pressure. In this case, the weak welding effect between chambers has a significant impact on product quality. The tiny leaks on the weak welding rods (usually called "cold welding") will directly lead to unexpected solution penetration between chambers during storage. However, there are currently no guidelines for testing the sealing of weak welding between chambers of multi-chamber products, and there are few related research reports. The sealing of the overall packaging system of the product is often tested by methods such as microbial invasion, colored water immersion, vacuum decay, and high-voltage discharge. However, such methods can only detect the sealing of the overall product and cannot detect leakage at the location of the weak welding rods between chambers.

[0004] At present, the evaluation of weak welding effect is mostly based on opening force test or peeling strength test. The opening force test is used to show that the product can open multiple chambers when using considerable force to meet the application requirements of the product. Although the peeling strength test of sampling detection can evaluate the overall sealing strength of the weak welding sealing strip, the test results cannot provide an effective basis for judging whether there are tiny leaks in the weak welding sealing strip that are invisible to the naked eye. Such leaks may cause the product to mix between multiple chambers during storage, resulting in quality failure. In addition, non-100% full detection has the defect of insufficient representativeness, especially for the case where multiple chambers are all colorless liquids. The insufficient sealing of the weak welding will cause unexpected premature mixing of the liquids between the chambers, affecting the product quality, thereby leading to possible safety problems. Summary of the invention

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a method for detecting the sealing of weak welds between chambers of a multi-chamber product. The inventors have found that by filling a commonly used microbial culture medium in one chamber of a multi-chamber packaging material and a bacterial suspension in another adjacent chamber, and then culturing the filled samples at an appropriate temperature, it is possible to determine whether the weak welds between chambers are completely sealed by observing whether the chamber culture medium filled with the microbial culture medium is turbid and bacteria grow. This method can detect tiny leaks on weak welding rods that are invisible to the naked eye, solving the dilemma of the current multi-chamber product having no method for detecting the sealing of weak welds between chambers, and is of great significance for the development of multi-chamber products.

[0006] In a first aspect, the present invention provides a method for determining the sealing performance of a weak weld between chambers of a multi-chamber product, comprising the following steps:

[0007] (1) providing the multi-chamber product, wherein the multi-chamber product comprises at least a first chamber and a second chamber, the first chamber and the second chamber are separated by a weak welding rod, the first chamber is filled with a bacterial suspension, and the second chamber is filled with a microbial culture medium;

[0008] (2) incubating the multi-chamber product at a predetermined temperature for a predetermined time; and

[0009] (3) Observe whether the culture medium in the second chamber after culture is turbid to determine the sealing performance of the multi-chamber product.

[0010] First, for step (1), as used herein, the term "weak welding" refers to a fine welding process, which refers to the use of finely controlled welding parameters to achieve high-precision welding, and is generally used for small parts or high-precision applications. For example, in a preferred embodiment of the present invention, the weak welding rod can be formed by ultrasonic welding, laser welding, resistance welding, high-frequency induction welding or electron beam welding, but is not limited thereto. In addition, the term "weak welding rod" refers to the welding rod formed at the connection by the weak welding process.

[0011] In another preferred embodiment of the present invention, the bacterial species in the bacterial suspension can be selected from one or more of Pseudomonas diminuta (diameter of about 0.5-0.8 μm), Serratia marcescens (diameter of about 0.5-1 μm) and Pseudomonas aeruginosa (diameter of about 0.5-1 μm). Furthermore, in order to ensure the sensitivity of the above method of the present invention, the bacterial concentration in the bacterial suspension can be not less than 1 x 10 5 cfu / mL (e.g. 2x 10 5 cfu / mL, 5x 10 5 cfu / mL or 1x 10 6 cfu / mL, etc.).

[0012] According to the present invention, the culture medium can be any culture medium suitable for the growth of the strains in the bacterial suspension, and the components and contents in the culture medium can be adjusted according to the strains selected as above, which is entirely within the capabilities of those skilled in the art. In a preferred embodiment of the present invention, the microbial culture medium can be trypticase soy broth (TSB), but is not limited thereto.

[0013] Secondly, for step (2), the multi-chamber product can be placed at a predetermined temperature suitable for the growth of the bacterial species and cultured for a predetermined time, so as to subsequently observe and determine whether the bacterial suspension enters the culture medium for growth, thereby determining the sealing of the weak weld. It should also be understood that the selection of the predetermined temperature and the predetermined time can be adjusted according to parameters such as the selected different bacterial species and different culture media, and in order to improve the accuracy of the test results, it can be considered to appropriately increase the predetermined culture time.

[0014] Therefore, in a preferred embodiment of the present invention, the predetermined temperature may be a temperature suitable for the growth of the bacterial species in the bacterial suspension, preferably in the range of 28-37°C, more preferably in the range of 30-35°C, such as 31°C, 32°C, 33°C or 34°C, etc. In another preferred embodiment of the present invention, the predetermined time may be 2-14 days, preferably 7-10 days (e.g. 7 days, 8 days, 9 days or 10 days, etc.).

[0015] Finally, for step (3), as a result of the method for determining the sealing performance of the weak weld between chambers of a multi-chamber product of the present invention, it is possible to determine whether the weak weld between chambers is completely sealed, for example, by observing whether the chamber culture medium filled with the microbial culture medium is turbid and bacteria grow, and according to actual needs, it is possible to further consider qualitatively or quantitatively judging the sealing performance of the weak weld between chambers of the multi-chamber product. For example, the qualitative judgment can be to qualitatively judge the sealing performance of the weak weld between chambers of the multi-chamber product by observing whether the culture medium in the second chamber is turbid or the degree of turbidity, and the quantitative judgment can be to quantitatively judge the sealing performance of the weak weld between chambers of the multi-chamber product by measuring the bacterial concentration in the culture medium in the second chamber.

[0016] Therefore, in a preferred embodiment of the present invention, when the culture medium in the second chamber after culture is observed to be turbid, it is determined that the sealing performance of the multi-chamber product is poor or does not meet the requirements. In another preferred embodiment of the present invention, when the culture medium in the second chamber after culture is observed to be not turbid, it is determined that the sealing performance of the multi-chamber product is good or meets the requirements.

[0017] In addition, although the method mentioned above in this article can effectively determine the weak weld sealing between chambers of multi-chamber products, considering that multi-chamber products may be stored in different environments due to different contents (such as medicines), especially under some harsh conditions, this will put higher requirements on the sealing of multi-chamber products.

[0018] Therefore, in the inventor's further research, in order to ensure that the multi-chamber product can meet the stability requirements under harsh conditions (such as high pressure conditions), harsh conditions can be optionally added to the multi-chamber product in the process of testing the weak welding sealing between chambers of the multi-chamber product. For example, in a preferred embodiment of the present invention, the content in at least one of the first chamber and the second chamber can be exerted with a force toward the weak welding rod, and more specifically, at least one of the first chamber and the second chamber can be set to a negative pressure state, such as by vacuuming.

[0019] However, considering that the chamber itself is sealed, the operation of vacuuming the chamber is difficult and complicated, and compared with the single-chamber product, and because the weak welding rod of the multi-chamber product is inside the multi-chamber product, the test of the multi-chamber product cannot test the sealing of the weak welding rod by vacuuming the entire external environment of the chamber product. As a solution, in a preferred embodiment of the present invention, at least one of the first chamber and the second chamber is clamped by a clamp. Since the clamp can compress the volume of the chamber, this will cause the contents in the chamber to be exerted with a force toward the weak welding rod.

[0020] Based on the above scheme of the present invention, the inventors were surprised to find that compared with the color water method that is easy to think of in the sealing detection, the method of the present invention has higher sensitivity, can detect leaks with smaller apertures in the sealing strip, and is more suitable for weak welding sealing detection between chambers of multi-chamber products. In addition, the method can be used to guide the development of weak welding sealing process parameters and verify the weak welding sealing process. Samples prepared with different process parameters may have different leakage situations with different sealing strengths. The weak welding sealing evaluation method of multi-chamber products is used for detection, and the weak welding process parameters with ideal sealing strength and no leaks can be determined. The method can also be used for weak welding process verification, which is used to verify whether the weak welding process can continuously and stably produce multi-chamber products with weak welding rods and no leaks.

[0021] Therefore, in a second aspect, the present invention also provides a method for producing a multi-chamber product, which comprises the following steps: providing a first chamber and a second chamber; performing weak welding on the first chamber and the second chamber using different weak welding parameters to form a multi-chamber product including the first chamber and the second chamber; determining the weak welding sealing between chambers of the multi-chamber product using the method of the first aspect above; when the weak welding sealing between chambers meets the requirements, producing the multi-chamber product using weak welding parameters that achieve the weak welding sealing between chambers.

[0022] The inventors further optimized the weak welding parameters by ultrasonic welding as a representative weak welding process in the embodiments described in detail below. As a result, in a preferred embodiment of the present invention, the conditions of the ultrasonic welding may include an amplitude of more than 85% and an energy of more than 70J. The inventors found that when the multi-chamber product is weakly welded by ultrasonic welding with such conditions, the requirements for weak welding sealing can be met. DETAILED DESCRIPTION

[0023] The specific embodiments of the present invention are described in detail below. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0024] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0025] Example

[0026] Example 1

[0027] Since the weak welding sealing strip cannot be artificially welded to create a tiny leak, the leak of different sizes is created in the sealed container. Specifically, the internal chamber of the container simulates the chamber 1 of the multi-chamber product filled with the reference liquid, and the sealing tester container simulates the chamber 2 of the multi-chamber product filled with the indicator liquid, so as to study the detection sensitivity of different methods. The results show that the method of filling one end with culture medium and the other end with bacterial suspension has the highest detection sensitivity. The specific experiment is as follows.

[0028] The method of the present invention is as follows: TSB culture medium is filled in a plastic container, and a leak test is performed to determine that it is completely sealed. Samples with different pore sizes of 10 μm, 20 μm, and 30 μm are prepared by punching, with 10 samples of each pore size. The sample number is immersed in a sealing tester container filled with a suspension of Pseudomonas aeruginosa, and the parameters are set to a negative pressure of -20 kPa for 10 minutes, and then maintained at normal pressure for 1 minute. The sample is taken out and placed at 30-35°C for 7 days to observe the color change of the reference solution. The pore size corresponding to the turbidity and bacterial growth of the 10 samples corresponds to the sensitivity of the method, see Table 1 for details.

[0029] Comparative method 1: Fill a plastic container with purified water and check for leaks to ensure complete sealing. Punch holes to prepare samples with different pore sizes of 10μm, 20μm, and 30μm, with 10 samples of each pore size. Immerse the sample number in a sealing tester container filled with methylene blue solution, set the parameters to negative pressure of -20kPa for 10 minutes, then maintain normal pressure for 1 minute, take out the sample and observe the color change. The pore size corresponding to the blue color of all 10 samples corresponds to the sensitivity of the method, see Table 1 for details.

[0030] Comparative method 2: Fill a plastic container with water of pH 9 and check for leaks to ensure complete sealing. Prepare samples of different pore sizes of 10μm, 20μm, and 30μm by punching, with 10 samples of each pore size. Immerse the sample number in a sealing tester container filled with phenolphthalein solution, set the parameters to negative pressure of -20kPa for 10 minutes, and then maintain at normal pressure for 1 minute. Take out the sample and observe the color change. The pore size corresponding to which all 10 samples turn red corresponds to the sensitivity of the method, see Table 1 for details.

[0031] Table 1

[0032] Different detection methods TSB+bacterial suspension Water + Methylene Blue pH 9 water + phenolphthalein 10μm leak 10 long mushrooms 6 turns blue 7 turns red 20μm leak 10 long mushrooms 9 turns blue 10 turns red 30μm leak 10 long mushrooms 10 turns blue 10 turns red Sensitivity <10μm 30μm 20μm

[0033] As can be seen from Table 1, when the leak hole size is 30 μm, the method of the present invention and comparative methods 1-2 all meet the sensitivity requirements; when the leak hole size is 20 μm, comparative method 1 no longer meets the sensitivity requirements; and when the leak hole size is 10 μm, comparative method 2 no longer meets the sensitivity requirements, while the method of the present invention still shows 10 long bacteria, that is, it meets the sensitivity requirements. Since no rigorous experiments with smaller sizes were conducted, it can be judged that the sensitivity of the method of the present invention is at least 10 μm or even lower.

[0034] Example 2

[0035] Since the same sealing detection method may have different sensitivities under different detection conditions, different detection conditions are used in this embodiment to further verify the sensitivity of the sealing detection method. In the microbial invasion method, vacuuming or pressurizing during the immersion of the bacterial suspension can increase the probability of microorganisms passing through the leak hole, thereby achieving the effect of improving the sensitivity of the method.

[0036] Method 1: Fill a plastic container with TSB culture medium and check for leaks to ensure it is completely sealed. Punch holes to prepare samples with different pore sizes of 10 μm, 15 μm, and 20 μm, with 10 tubes for each pore size. Immerse the sample number in a container filled with Pseudomonas aeruginosa suspension (the concentration of the suspension should be no less than 10 5 cfu / ml), set the parameters to negative pressure of -20kPa for 10 minutes, then maintain normal pressure for 1 minute, take out the sample and place it at 30-35℃ for 7 days to observe the color change of the reference solution. The aperture corresponding to the growth of bacteria in all 10 samples is the sensitivity of the method.

[0037] Method 2: Fill a plastic container with TSB culture medium and check for leaks to ensure it is completely sealed. Punch holes to prepare samples with different pore sizes of 10μm, 15μm, and 20μm, with 10 samples for each pore size. Immerse the sample number in a seal tester container filled with a suspension of Pseudomonas aeruginosa (the concentration of the suspension should be no less than 105cfu / ml), set the parameters to a positive pressure of 20kPa for 10 minutes, and then maintain normal pressure for 1 minute. Take out the sample and place it at 30-35℃ for 7 days to observe the color change of the reference solution. The pore size corresponding to the growth of bacteria in all 10 samples is the sensitivity of the method.

[0038] Method 3: Fill a plastic container with TSB culture medium and check for leaks to ensure it is completely sealed. Punch holes to prepare samples with different pore sizes of 10μm, 15μm, and 20μm, with 10 samples for each pore size. Immerse the sample number in a seal tester container filled with a suspension of Pseudomonas aeruginosa (the concentration of the suspension should be no less than 105cfu / ml), set the parameters to maintain normal pressure for 20 minutes, take out the sample and place it at 30-35℃ for 7 days to observe the color change of the reference solution. The pore size corresponding to the growth of bacteria in all 10 samples is the sensitivity of the method.

[0039] Refer to Table 2 below. By comparing the sensitivity of the microbial invasion method under different detection conditions, it can be seen that when negative or positive pressure is applied during the immersion process, the sensitivity of the method can reach 10μm. If it is only immersed at normal pressure, the sensitivity is poor, only 15μm.

[0040] Table 2

[0041] Different testing conditions Negative pressure immersion (method 1) Positive pressure immersion (method 2) Normal pressure immersion (method 3) 10μm leak 10 long mushrooms 10 long mushrooms 7 long mushrooms 15μm leak 10 long mushrooms 10 long mushrooms 10 long mushrooms 20μm leak 10 long mushrooms 10 long mushrooms 10 long mushrooms Sensitivity 10μm 10μm 15μm

[0042] Example 3

[0043] Comparison of qualified rates of different testing methods:

[0044] Method 1 of the present invention: chamber 1 is filled with 0.5 ml of the reference solution tryptic soy bean broth (TSB), and chamber 2 is filled with 0.5 ml of the indicator solution Pseudomonas aeruginosa suspension (the concentration of the suspension should be no less than 10 5cfu / ml), and samples were prepared using different ultrasonic weak welding parameters, with 50 samples for each parameter. Chamber 2 was clamped with an appropriate fixture for 30 minutes, and then the fixture was removed (the fixture should be able to provide appropriate pressure but not damage the weak welding rod). It was placed at 30-35°C for 7 days to observe whether the culture medium was turbid and bacteria grew. Samples with bacteria were unqualified.

[0045] Comparative method 2: chamber 1 is filled with 0.5 ml of purified water, chamber 2 is filled with 0.5 ml of methylene blue solution, and the rest is the same as the method of the present invention. Finally, visually observe whether chamber 1 turns blue. If it turns blue, it is a defective product.

[0046] Comparative method 3: Chamber 1 is filled with 0.5 ml of the reference solution, tryptic soy broth (TSB), and chamber 2 is filled with 0.5 ml of the indicator solution, Pseudomonas aeruginosa suspension (the concentration of the suspension should be no less than 10 5 cfu / ml), and different ultrasonic weak welding parameters were used to prepare samples, with 50 samples for each parameter. The samples were placed at 30-35℃ for 7 days to observe whether the culture medium was turbid and bacteria grew. Samples with bacteria were unqualified.

[0047] Table 3

[0048]

[0049] From the results in Table 3, it can be seen that when the ultrasonic weak welding parameters are small (amplitude 70% / energy 60J, or amplitude 85% / energy 60J), the welding effect does not reach the ideal state, and there are some tiny leaks. The comparative methods (color water method and microbiological method without fixture) cannot detect all defective products due to sensitivity deviation. In contrast, the high-sensitivity implementation method can better identify possible defective products and is more instructive for the development of welding and sealing process parameters.

[0050] Example 4

[0051] Weak welding parameter investigation:

[0052] Group 1: Chamber 1 is filled with 0.5 ml of the reference solution, tryptic soy broth (TSB), and chamber 2 is filled with 0.5 ml of the indicator solution, Pseudomonas aeruginosa suspension (the concentration of the suspension should be no less than 10 5 cfu / ml), and different ultrasonic weak welding parameters were used to prepare samples, with 50 samples for each parameter. Group 2: Using the same ultrasonic weak welding parameters, chamber 2 was filled with 0.5ml of the reference solution, tryptic soy bean broth (TSB), and chamber 1 was filled with 0.5ml of the indicator solution, Pseudomonas aeruginosa suspension (the concentration of the suspension should not be less than 10 5 cfu / ml), and 50 samples were prepared for each parameter.

[0053] The samples were placed at 30-35°C for 7 days to observe whether the culture medium was turbid and bacteria grew. The number of unqualified samples was used to determine whether the weak weld parameters met the sealing requirements. The results are shown in Table 4 below.

[0054] Table 4

[0055]

[0056] From the results in Table 4, it can be seen that when the ultrasonic weak welding parameters are amplitude 85%, energy 70J and 90J, the sealing performance of the welding strip meets the requirements without leakage.

[0057] In addition, the samples of each group of weak welding rods with qualified sealing properties were placed in the sealing instrument cavity, and Pseudomonas aeruginosa suspension was added (the concentration of the suspension should be no less than 10 5 cfu / ml) until it covers all samples. Under negative pressure of -20kPa, for 10min, then maintain at normal pressure for 1min. Take out the sample, clean its outer surface with 75% ethanol, place it at 30-35℃ and culture for 7 days to observe whether the culture medium is turbid and bacteria grow. The amount of unqualified samples is used to determine whether the sample has leaks around the welding rod due to excessive welding. The results are shown in Table 5 below.

[0058] Table 5

[0059]

[0060]

[0061] From the results in Table 5, it can be seen that when the ultrasonic weak welding parameters are amplitude 85% and energy 60J and 70J, there is no leakage around the welding strip caused by excessive welding, and the sealing meets the requirements.

[0062] Based on the data of the above two experiments, when the ultrasonic weak welding parameters are 85% amplitude and 70J energy, there is no leakage in the welding strip and there is no leakage around the welding strip. This parameter can ensure the sealing of the two chambers.

[0063] Example 5

[0064] In order to verify that the optimized cold welding process can meet the production requirements, the optimized ultrasonic weak welding parameters with an amplitude of 85% and an energy of 70J were used to carry out three batches (5000 tubes in a batch). One chamber was filled with 0.5ml of the reference liquid tryptic soy bean broth (TSB), and the other chamber was filled with 0.5ml of the indicator liquid Pseudomonas aeruginosa suspension (the concentration of the bacterial suspension was not less than 10 5 cfu / ml), all samples of the three batches were placed at 30-35℃ for 7 days to observe whether the culture medium was turbid and bacteria grew. The number of unqualified samples was used to determine whether the cold soldering process verification was passed (the proposed unqualified rate should not be higher than 0.1%), and the results are shown in Table 6 below.

[0065] Table 6

[0066] batch Batch 1 Batch 2 Batch 3 Batch (pieces) 5000 5000 5000 Number of unqualified items 2 0 4 Failure rate (%) 0.04％ 0 0.08％

[0067] It can be seen from Table 6 that the unqualified rates of the three batches of samples are all less than 0.1%, thus confirming that the optimized weak welding process can continuously and stably produce multi-chamber products with good weak welding rod sealing.

[0068] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0069] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

[0070] In addition, various embodiments of the present invention may be arbitrarily combined, and as long as they do not violate the concept of the present invention, they should also be regarded as the contents disclosed by the present invention.

Claims

1. A method for determining the sealing performance of a weak weld between chambers of a multi-chamber product, comprising the following steps: (1) providing the multi-chamber product, wherein the multi-chamber product comprises at least a first chamber and a second chamber, the first chamber and the second chamber are separated by a weak welding rod, the first chamber is filled with a bacterial suspension, and the second chamber is filled with a microbial culture medium; (2) incubating the multi-chamber product at a predetermined temperature for a predetermined time; as well as (3) Observe whether the culture medium in the second chamber after culture is turbid to determine the sealing performance of the multi-chamber product.

2. The method according to claim 1, wherein: The weak welding rod is formed by ultrasonic welding, laser welding, resistance welding, high frequency induction welding or electron beam welding.

3. The method according to claim 1, wherein: The bacterial species in the bacterial suspension are selected from one or more of Pseudomonas diminuta, Serratia marcescens and Pseudomonas aeruginosa.

4. The method according to claim 1, wherein: The bacterial concentration in the bacterial suspension is not less than 1×10 5 cfu / mL.

5. The method according to claim 1, wherein: The culture medium was Trypticase Soy Broth (TSB).

6. The method according to claim 1, wherein: The predetermined temperature is a temperature suitable for the growth of the bacteria in the bacterial suspension, preferably in the range of 28-37°C, more preferably in the range of 30-35°C.

7. The method according to claim 1, wherein: The predetermined time is 2-14 days, preferably 7-10 days.

8. The method according to claim 1, wherein: When the culture medium in the second chamber after culture is observed to be turbid, it is determined that the sealing property of the multi-chamber product is poor.

9. The method according to claim 1, wherein: When it is observed that the culture medium in the second chamber after culture is not turbid, it is determined that the sealing property of the multi-chamber product is good.

10. The method according to any one of claims 1 to 9, wherein: The contents of at least one of the first chamber and the second chamber are forced toward the weak electrode.

11. The method according to claim 10, wherein: A pressure differential is created between the first chamber and the second chamber to apply a force toward the weak electrode to the contents of at least one of the first chamber and the second chamber.

12. The method according to claim 11, wherein: At least one of the first chamber and the second chamber is in a negative pressure state.

13. The method according to claim 10, wherein: At least one of the first chamber and the second chamber is clamped by a clamp.

14. A method for producing a multi-chamber product, comprising the steps of: providing a first chamber and a second chamber; Weak welding the first cavity and the second cavity using different weak welding parameters to form a multi-cavity product including the first cavity and the second cavity; Determining the inter-chamber weak weld sealing of the multi-chamber product using the method according to any one of claims 1-13; When the weak weld sealing performance between the chambers meets the requirements, the multi-chamber product is produced using weak weld parameters that achieve the weak weld sealing performance between the chambers.

15. The method according to claim 14, wherein: The weak weld is ultrasonic weld.

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