Automatic high-precision confocal laser scanning microscope

Abstract

The invention relates to an automatic high-precision confocal laser scanning microscope, which belongs to the technical field of medical instruments and comprises an operation bottom plate, a finger clamping fixer, a visual angle compensation camera, a path control rod, a confocal laser scanning microscope, an auxiliary positioning laser and a central controller. According to the invention, the automatic high-precision confocal laser scanning microscope with a finger fixing structure, a circuit control structure and an auxiliary scanning structure is arranged, and the fixed base is combined with a surrounding type inflatable fixing belt, so that a finger can be stably and firmly clamped and limited, and the finger is prevented from shaking in the whole detection process; the multi-axis route control rod with the displacement sensor is combined with the central controller, accurate route measurement recording can be achieved, set route advancing can be repeatedly and stably achieved, the camera is combined with the auxiliary laser irradiator, the accuracy of auxiliary determination of route irradiation points can be guaranteed, and the work efficiency is improved. And finally, accurate, efficient and stable confocal laser scanning microscope examination is realized.

Description

Technical Field

[0001] The invention relates to the technical field of medical instruments, in particular to an automated high-precision confocal laser scanning microscope. Background Art

[0002] Confocal laser scanning microscopy is a method of scanning the skin with a confocal laser scanning microscope to obtain images of the skin's hierarchical structure, which can be used to diagnose skin tumors, skin scars, skin cysts, and other diseases. Generally, a special instrument is used during the examination, and after several scans, the skin's hierarchical structure can be clearly observed, thereby diagnosing the disease.

[0003] However, for the strip-like melanin plaques on the nails, it is very easy to fail to achieve stable and complete scanning back and forth several times due to the difficulty in aligning the irradiation device and the unstable holding of the medical staff during detection, resulting in inaccurate detection. Although the image observed by microscopic observation is very large, the image is an enlarged image. In fact, the point where the laser irradiates the plaque may be very small. Therefore, any slight shake will cause a slight shift in the laser scanning area. However, the slight shift in the area where the laser is aimed will result in a huge movement or even jump to another unobserved area in the actual image finally generated by the microscope, resulting in a discontinuous observation image. As a result, the irradiation path for the reciprocating detection needs to be re-determined, which greatly increases the difficulty of the detection operation. If a continuous and clear irradiation image cannot be obtained, the final test result will result in a diagnostic error. Summary of the invention

[0004] In view of this, the purpose of the present invention is to propose an automated high-precision confocal laser scanning microscope. By setting up an automated high-precision confocal laser scanning microscope with a finger fixing structure, a circuit control structure and an auxiliary scanning structure, the problem in the prior art that the patient's fingers are prone to shaking during detection, resulting in inaccurate detection results, and the manual assisted scanning by medical staff is difficult to operate and the scanning irradiation is unstable, resulting in detection errors affecting treatment.

[0005] The present invention is achieved through the following technical solutions:

[0006] An automated high-precision confocal laser scanning microscope comprises an operating base plate, a finger clamping fixture is fixedly arranged on the top of the operating base plate, a viewing angle compensation camera is fixedly arranged on the end of the finger clamping fixture, a path control rod is fixedly arranged on the rear side of the viewing angle compensation camera, a confocal laser scanning microscope is fixedly arranged on the lower end of the path control rod, an auxiliary positioning laser is fixedly arranged on the side of the confocal laser scanning microscope, a central controller is fixedly arranged on the right side of the finger clamping fixture, and the central controller is respectively connected to the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture through lines.

[0007] Furthermore, the path control rod includes an electric sleeve rotating base, an electric lifting Z-axis rod is fixedly arranged on the top of the electric sleeve rotating base, a longitudinal electric sliding sleeve is fixedly arranged on the top of the electric lifting Z-axis rod, an electric sliding Y-axis rod is slidably arranged inside the longitudinal electric sliding sleeve, a transverse electric sliding sleeve is fixedly arranged on the top of the electric sliding Y-axis rod, and an electric sliding X-axis rod is slidably arranged inside the transverse electric sliding sleeve.

[0008] Furthermore, the confocal laser scanning microscope is fixedly arranged at the bottom of one end of the electric sliding X-axis rod, the axis line between the auxiliary positioning laser and the confocal laser scanning microscope is parallel to the electric sliding Y-axis rod, and the distance between the auxiliary positioning laser and the axis of the confocal laser scanning microscope is fixed.

[0009] Furthermore, the finger gripper comprises a finger storage slot, and an inflatable fixing belt is fixedly provided on both sides of the finger storage slot. An inflatable motor is fixedly provided on the outer side of the finger storage slot, and the inflatable fixing belt is connected to the inflatable motor through a pipeline.

[0010] Furthermore, the inflatable fixing belt is made of skin-like material, and the surface of the inflatable fixing belt is provided with anti-slip wave dots.

[0011] Furthermore, the longitudinal electric sliding sleeve and the transverse electric sliding sleeve are both fixedly provided with displacement distance sensors, and the displacement distance sensors are connected to the central controller via wireless signals.

[0012] Furthermore, an anti-glare glass cover is fixedly provided on the upper end of the operating base plate, and the anti-glare glass cover covers the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture. The central controller is fixedly provided on the outside of the anti-glare glass cover, and an arm entrance and exit are opened on the front side of the anti-glare glass cover.

[0013] Furthermore, the path control rod, the auxiliary positioning laser and the finger clamping fixture are all sprayed with a radiation interference protection coating.

[0014] The beneficial effects of the present invention are:

[0015] The present invention provides an automated high-precision confocal laser scanning microscope that is simultaneously equipped with a finger fixing structure, a line control structure, and an auxiliary scanning structure. The fixed base is combined with a surrounding inflatable fixing belt to help steadily and firmly clamp and limit the fingers to ensure that the fingers do not shake during the entire detection process. The multi-axis route control lever with a displacement sensor is combined with a central controller to help achieve accurate path measurement and recording and repeatedly and stably achieve set path travel. The camera is combined with an auxiliary laser illuminator to help ensure the accuracy of the auxiliary determination of the route irradiation point, and ultimately achieve accurate, efficient and stable confocal laser scanning microscope inspection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is the overall structure diagram of the assembly;

[0017] Figure 2 This is the front view of the overall structure;

[0018] Figure 3 It is the side view of the overall structure;

[0019] Figure 4 It is a top view of the overall assembly;

[0020] Figure 5 To control the relationship diagram.

[0021] Description of reference numerals:

[0022] 1. Operating base plate; 2. Finger gripping fixture; 3. View compensation camera; 4. Path control lever; 5. Confocal laser scanning microscope; 6. Auxiliary positioning laser; 7. Center controller; 8. Electric sleeve rotating base; 9. Electric lifting Z-axis rod; 10. Longitudinal electric sliding sleeve; 11. Electric sliding Y-axis rod; 12. Horizontal electric sliding sleeve; 13. Electric sliding X-axis rod; 14. Finger storage slot; 15. Inflatable fixing belt; 16. Inflatable motor; 17. Displacement distance sensor. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0024] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0026] In the above description of the present invention, it should be noted that the terms "one side", "the other side", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the invention is usually placed when in use, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0027] In addition, the term "same" does not mean that the parts must be absolutely the same, but slight differences are allowed. The term "vertical" only means that the positional relationship between the parts is more vertical than "parallel", and does not mean that the structure must be completely vertical, but can be slightly tilted.

[0028] like Figure 1-5 As shown, an embodiment of the present invention is: an automated high-precision confocal laser scanning microscope, comprising an operating base plate 1, a finger clamping fixture 2 is fixedly arranged on the top of the operating base plate, a viewing angle compensation camera 3 is fixedly arranged at the end of the finger clamping fixture, a path control rod 4 is fixedly arranged on the rear side of the viewing angle compensation camera, a confocal laser scanning microscope 5 is fixedly arranged at the lower end of the path control rod, an auxiliary positioning laser 6 is fixedly arranged on the side of the confocal laser scanning microscope, and a central controller 7 is fixedly arranged on the right side of the finger clamp, and the central controller is respectively connected to the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture through lines.

[0029] Among them, the path control rod includes an electric sleeve rotating base 8, an electric lifting Z-axis rod 9 is fixedly arranged on the top of the electric sleeve rotating base, a longitudinal electric sliding sleeve 10 is fixedly arranged on the top of the electric lifting Z-axis rod, an electric sliding Y-axis rod 11 is slidably arranged inside the longitudinal electric sliding sleeve, a transverse electric sliding sleeve 12 is fixedly arranged on the top of the electric sliding Y-axis rod, and an electric sliding X-axis rod 13 is slidably arranged inside the transverse electric sliding sleeve.

[0030] The confocal laser scanning microscope is fixedly set at the bottom of one end of the electric sliding X-axis rod, the axis connecting the auxiliary positioning laser and the confocal laser scanning microscope is parallel to the electric sliding Y-axis rod, and the axis distance between the auxiliary positioning laser and the confocal laser scanning microscope is fixed. The purpose of this setting is that the light emitted by the confocal laser scanning microscope is not obvious and is used for alignment to confirm that the path is inaccurate, but the auxiliary positioning laser can emit obvious light to form a light spot that falls on the nail, so that the path can be clearly obtained. The purpose of ensuring that the axis distance between the auxiliary positioning laser and the confocal laser scanning microscope is fixed and the axis connecting the auxiliary positioning laser and the confocal laser scanning microscope is parallel to the electric sliding Y-axis rod is to use the auxiliary positioning laser to indicate and record a detection path first, and then subtract the fixed distance value between the two in the Y-axis direction from the coordinate value, so as to obtain the accurate path coordinate value that the confocal laser scanning microscope needs to travel.

[0031] The finger clamp includes a finger storage slot 14, and an inflatable fixing belt 15 is fixedly installed on both sides of the finger storage slot. An inflatable motor 16 is fixedly installed on the outside of the finger storage slot. The inflatable fixing belt is connected to the inflatable motor through a pipeline. The inflatable fixing belt is made of skin-like material, and the surface of the inflatable fixing belt is provided with anti-slip wave dots. The longitudinal electric sliding sleeve and the transverse electric sliding sleeve are fixedly provided with a displacement distance sensor 17, and the displacement distance sensor is connected to the central controller through a wireless signal. The path control rod, the auxiliary positioning laser and the finger clamp fixture are all sprayed with an anti-radiation interference coating. This operation is used to ensure the normal use of the above instrument.

[0032] When implementing this embodiment, first let the patient place the finger on the finger clamping fixture, and then the central controller controls the inflation motor to inflate the inflatable fixing belt to fix the finger firmly to prevent shaking. After fixing the finger, the central controller controls the path control lever to drive the activated auxiliary positioning laser to irradiate the black striped plaque on the finger. First, locate a point on the edge of the plaque and set its position coordinate data as the zero point. Then, according to the movement of the picture sent back by the viewing angle compensation camera, the light spot irradiated by the auxiliary positioning laser moves accurately along the position of the plaque. The final route will completely cover the black plaque. After that, the coordinate data of the entire walking path is recorded in the center of the central controller. The irradiation path is recorded through the preliminary irradiation of the auxiliary positioning laser because the irradiation path can be more quickly and conveniently passed through the plaque covering the skin and nails through the guidance of the visual clear light spot. If the confocal laser scanning microscope is directly used for irradiation to determine the path, the image transmitted to the display screen of the central controller is a microscopic magnified image, and any slight shaking will cause microscopic The dramatic shift of the magnified image is therefore not suitable for the preliminary determination of the searchlight detection path. Therefore, here we first use the auxiliary positioning laser to perform external macroscopic observation of the plaque to determine the preliminary searchlight path. Then, the central controller records the coordinate data and subtracts the fixed value of the axial distance between the auxiliary positioning laser and the confocal laser scanning microscope on the basis of each data, so that the coordinate data of the auxiliary positioning laser's travel path is changed to the data of the confocal laser scanning microscope. Then, the central controller demonstrates the movement once according to the changed coordinate data. If shadow images are taken during the process, it proves that the path is available. The central controller then uses the data to scan the patient's plaque back and forth for multiple times to finally obtain accurate detection results. If the shadow images taken during the process are incoherent or jump changes occur in the middle, a confocal laser scanning microscope microscopic searchlight observation is performed at the incoherence or image jump change to correct the preliminary searchlight path and obtain a more accurate microscopic corrected observation path. Then, the microscopic corrected observation path is used for repeated automated scanning and observation inspection under the control of the central controller to finally complete the detection of the plaque.

[0033] This embodiment provides an automated high-precision confocal laser scanning microscope that simultaneously has a finger fixing structure, a line control structure, and an auxiliary scanning structure. The fixed base is combined with a surrounding inflatable fixing belt to help clamp and limit the fingers steadily and firmly to ensure that the fingers do not shake during the entire detection process. The multi-axis route control lever with a displacement sensor is combined with a central controller to facilitate accurate path measurement and recording and repeated and stable realization of the set path. The camera is combined with an auxiliary laser illuminator to ensure the accuracy of the auxiliary determination of the route irradiation point, thereby ultimately achieving accurate and efficient confocal laser scanning microscope inspection.

[0034] In this embodiment, an anti-glare glass cover is fixedly arranged at the upper end of the operating base plate, and the anti-glare glass cover covers the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture, and the central controller is fixedly arranged outside the anti-glare glass cover, and an arm entrance and exit are opened on the front side of the anti-glare glass cover. After all, the confocal laser scanning microscope examination also generates strong energy through laser irradiation, and long-term proximity will also cause a certain degree of damage to the eyes. Therefore, the anti-glare glass cover is set up without affecting the observation and inspection, and can also ensure the safety of medical personnel.

[0035] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. An automated high-precision confocal laser scanning microscope, characterized in that: It includes an operating base plate, a finger clamping fixture is fixedly arranged on the top of the operating base plate, a viewing angle compensation camera is fixedly arranged on the end of the finger clamping fixture, a path control rod is fixedly arranged on the rear side of the viewing angle compensation camera, a confocal laser scanning microscope is fixedly arranged on the lower end of the path control rod, an auxiliary positioning laser is fixedly arranged on the side of the confocal laser scanning microscope, a central controller is fixedly arranged on the right side of the finger clamping fixture, and the central controller is respectively connected to the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture through lines.

2. The automated high-precision confocal laser scanning microscope according to claim 1, characterized in that: The path control rod includes an electric sleeve rotating base, an electric lifting Z-axis rod is fixedly arranged on the top of the electric sleeve rotating base, a longitudinal electric sliding sleeve is fixedly arranged on the top of the electric lifting Z-axis rod, an electric sliding Y-axis rod is slidably arranged inside the longitudinal electric sliding sleeve, a transverse electric sliding sleeve is fixedly arranged on the top of the electric sliding Y-axis rod, and an electric sliding X-axis rod is slidably arranged inside the transverse electric sliding sleeve.

3. The automated high-precision confocal laser scanning microscope according to claim 2, characterized in that: The confocal laser scanning microscope is fixedly arranged at the bottom of one end of the electric sliding X-axis rod, the axis connecting the auxiliary positioning laser and the confocal laser scanning microscope is parallel to the electric sliding Y-axis rod, and the distance between the auxiliary positioning laser and the axis of the confocal laser scanning microscope is fixed.

4. The automated high-precision confocal laser scanning microscope according to claim 1, characterized in that: The finger gripper comprises a finger storage slot, and both left and right sides of the finger storage slot are fixedly provided with inflatable fixing belts, an inflatable motor is fixedly provided outside the finger storage slot, and the inflatable fixing belt is connected with the inflatable motor through a pipeline.

5. The automated high-precision confocal laser scanning microscope according to claim 4, characterized in that: The inflatable fixing belt is made of skin-like material, and the surface of the inflatable fixing belt is provided with anti-slip wave dots.

6. The automated high-precision confocal laser scanning microscope according to claim 2, characterized in that: The longitudinal electric sliding sleeve and the transverse electric sliding sleeve are both fixedly provided with displacement distance sensors, and the displacement distance sensors are connected to the central controller via wireless signals.

7. The automated high-precision confocal laser scanning microscope according to claim 1, characterized in that An anti-glare glass cover is fixedly arranged on the upper end of the operating base plate, and the anti-glare glass cover covers the path control rod, the confocal laser scanning microscope, the auxiliary positioning laser and the finger clamping fixture. The central controller is fixedly arranged outside the anti-glare glass cover, and an arm entrance and exit are opened on the front side of the anti-glare glass cover.

8. The automated high-precision confocal laser scanning microscope according to claim 1, characterized in that The path control rod, auxiliary positioning laser and finger clamping fixture are all sprayed with anti-radiation interference coating.

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