Pest control composition

Abstract

The present invention provides a pest control composition, the pest control composition comprising: humic acid; a polymer having a weight average molecular weight of from 10,000 Daltons to 30,000 Daltons and containing from 20% by weight to 100% by weight of a monomer structural unit derived from a monomer having a log P of from 2.0 to 6.0; bacillus thuringiensis; and water.

Description

Technical Field

[0001] The present disclosure relates generally to pest control compositions, and more particularly to pest control compositions comprising humic acid. Background Art

[0002] Pest control agents are used to control pests, such as insects. The effectiveness of a pest control agent can be affected by a variety of factors. Industry is continually striving to develop new and improved pest control compositions. Summary of the invention

[0003] According to the first feature of the present disclosure, a pest control composition comprises: humic acid; a polymer, wherein the polymer has a weight average molecular weight of 10,000 to 30,000 Daltons and contains 20% to 100% by weight of monomer structural units derived from monomers having a log P of 2.0 to 6.0; Bacillus thuringiensis; and water. According to the second feature of the present disclosure, based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water, humic acid accounts for 0.010% to 5.000% by weight of the composition. According to the third feature of the present disclosure, based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water, the polymer accounts for 0.10% to 20.00% by weight of the composition. According to the fourth feature of the present disclosure, the polymer is a copolymer of diisobutylene and maleic anhydride. According to the fifth feature of the present disclosure, humic acid has a phenolic group concentration of 0.35 mmol / g humic acid to 0.45 mmol / g humic acid. DETAILED DESCRIPTION

[0004] As used herein, the term "and / or," when used in a list of two or more items, means that any one of the listed items may be used alone, or any combination of two or more of the listed items may be used. For example, if a composition is described as containing components A, B, and / or C, the composition may contain A alone; B alone; C alone; A and B in combination; A and C in combination; B and C in combination; or A, B, and C in combination.

[0005] Unless otherwise indicated, all ranges are inclusive. Subscript values ​​in polymer formulas refer to the molar average of the designated components of the polymer.

[0006] Test methods refer to the most recent test methods as of the priority date of this document, unless the date is expressed as a two-digit number with a hyphen in the test method number. Reference to a test method includes reference to both the testing association and the test method number. Test method organizations are referenced by one of the following abbreviations: ASTM refers to ASTM International (formerly the American Society for Testing and Materials); EN refers to European Standards; DIN refers to the German Institute for Standardization; and ISO refers to the International Organization for Standardization.

[0007] As used herein, unless specifically stated to the contrary, "wt %" or "weight percent" or "weight %" of a component is based on the total weight of the composition or article including the component. Unless otherwise indicated, all percentages are by weight.

[0008] Disclosed herein are pest control compositions. Embodiments of the present disclosure provide pest control compositions comprising a polymer, humic acid, water, and Bacillus thuringiensis.

[0009] The pest control composition disclosed herein can be applied to plants, such as plant surfaces, to control pests. Advantageously, the pest control composition disclosed herein can provide improved (i.e., higher) Bacillus thuringiensis activity after exposure to light (e.g., sunlight) compared to other formulations. The Bacillus thuringiensis activity shows that the pest control composition disclosed herein can provide improved pest control compared to other formulations. Surprisingly, the components of the pest control composition disclosed herein, i.e., humic acid, polymer and Bacillus thuringiensis, show unexpected synergistic effects on improving Bacillus thuringiensis activity after exposure to light.

[0010] The pest control composition disclosed herein comprises humic acid. Humic acid is an acidic organic polymer that can be extracted from humus in soil, sediment or aquatic environment. Humic acid is identified by Chemical Abstract Service (CAS) number 1415-93-6 and has an average chemical formula C 187 H 186 O 89 N9S1. Humic acid is commercially available. Examples of commercially available humic acid include, but are not limited to, products available from Sigma-Aldrich or Fisher Scientific.

[0011] The humic acid provided by one or more embodiments of the present disclosure can be a solid, for example, it is incorporated into the pest control composition disclosed herein. The humic acid provided by one or more embodiments of the present disclosure can be a mixture, for example, a mixture of humic acid and a liquid (such as water), which is incorporated into the pest control composition disclosed herein. The humic acid provided by one or more embodiments of the present disclosure can be a solution, for example, a solution of humic acid in a liquid (such as water), which is incorporated into the pest control composition disclosed herein. A combination of solids, mixtures and solutions can be used. Humic acid can have various pH values ​​for various applications.

[0012] Humic acid comprises phenolic groups. Humic acid can have a phenolic group concentration of 0.30 mmol / g humic acid to 0.50 mmol / g humic acid, as determined according to the procedure listed in the Examples section below. For example, humic acid can have a phenolic group concentration of 0.30 or greater, or 0.31 or greater, or 0.32 or greater, or 0.33 or greater, or 0.34 or greater, or 0.35 or greater, or 0.36 or greater, or 0.37 or greater, or 0.375 or greater, or 0.38 or greater, or 0.39 or greater, or 0.40 or greater, or 0.41 or greater, or 0.42 or greater, or 0.43 or greater, or 0.44 or greater, or 0.45 or greater, or 0.46 or greater, or 0.47 or greater, or 0.48 or greater, or 0.49 or greater, or 10.10 or greater. 9 or more, while at the same time, a phenolic concentration of 0.50 or less, or 0.49 or less, or 0.48 or less, or 0.47 or less, or 0.46 or less, or 0.45 or less, or 0.44 or less, or 0.43 or less, or 0.42 or less, or 0.41 or less, or 0.40 or less, or 0.39 or less, or 0.38 or less, or 0.375 or less, or 0.37 or less, or 0.36 or less, or 0.35 or less, or 0.34 or less, or 0.33 or less, or 0.32 or less, or 0.31 or less.

[0013] The pest control composition disclosed herein may include a polymer. As used herein, unless otherwise specified, "one" refers to one or more. As used herein, a "polymer" has two or more identical or different monomer structural units derived from two or more different monomers, such as copolymers, terpolymers, etc. As used herein, "monomer structural unit" with respect to a polymer represents a part of a polymer structure produced by one or more monomer reactions to form a polymer. "Different" with respect to a monomer structural unit indicates that the monomer structural units differ from each other by at least one atom or isomer. The embodiments of the present disclosure provide that the monomer structural units of a polymer are produced by the polymerization reaction of a monomer, i.e., formed. The monomer structural units provided by one or more embodiments may undergo one or more reactions, such as a hydrolysis reaction, after the polymerization reaction.

[0014] The polymers provided by the embodiments of the present disclosure contain 20 wt% to 100 wt% of monomer structural units derived from monomers (i.e., one or more monomers) having a log P of 2.0 to 6.0, based on the total weight of the polymer. The polymer may contain more than 90 wt% of monomer structural units derived from monomers having a log P greater than 1.0.

[0015] One or more of the monomer structural units may have 1.0 or more, or 1.2 or more, or 1.4 or more, or 1.6 or more, or 1.8 or more, or 2.0 or more, or 2.2 or more, or 2.4 or more, or 2.6 or more, or 2.8 or more, or 3.0 or more, or 3.2 or more, or 3.4 or more, or 3.6 or more, or 3.8 or more, or 4.0 or more, or 4.2 or more, or 4.4 or more, or 4.6 or more, or 4.8 or greater, while at the same time, 5.0 or less, or 4.8 or less, or 4.6 or less, or 4.4 or less, or 4.2 or less, or 4.0 or less, or 3.8 or less, or 3.6 or less, or 3.4 or less, or 3.2 or less, or 3.0 or less, or 2.8 or less, or 2.6 or less, or 2.4 or less, or 2.2 or less, or 2.0 or less, or 1.8 or less, or 1.6 or less, or 1.4 or less, or 1.2 or less of log P. Log P values ​​were obtained by utilizing the Evaluation Program Interface (EPI) Suite available at https: / / www.epa.gov / tsca-screening-tools / epi-suitetm-estimation-program-interface TM (KOWWIN version 1.68) to determine.

[0016] Exemplary monomers with log P for the polymer include diisobutylene (log P of 4.08), butyl methacrylate (log P of 2.75), butyl acrylate (log P of 2.20), methyl methacrylate (log P of 1.28), ethyl acrylate (log P of 1.22), 2-ethylhexyl acrylate (log P of 4.09), styrene (log P of 2.89), maleic anhydride (log P of 1.62), behenyl methacrylate (log P of 11.59), and combinations thereof.

[0017] As described above, the polymer may contain from 20% to 100% by weight of monomer structural units derived from monomers having a log P of 2.0 to 6.0. For example, the polymer may contain 20% by weight or more, or 25% by weight or more, or 30% by weight or more, or 35% by weight or more, or 40% by weight or more, or 45% by weight or more, or 50% by weight or more, or 55% by weight or more, or 60% by weight or more, or 65% by weight or more, or 70% by weight or more, or 75% by weight or more, or 80% by weight or more, or 85% by weight or more, or 90% by weight or more, or 95% by weight or more, While at the same time, 100 wt % or less, or 95 wt % or less, or 90 wt % or less, or 85 wt % or less, or 80 wt % or less, or 75 wt % or less, or 70 wt % or less, or 65 wt % or less, or 60 wt % or less, or 55 wt % or less, or 50 wt % or less, or 45 wt % or less, or 40 wt % or less, or 35 wt % or less, or 30 wt % or less, or 25 wt % or less of monomer structural units derived from monomers having a log P of 2.0 to 6.0.

[0018] The polymer may contain 90% by weight of monomer structural units derived from monomers having a log P greater than or equal to 1.0. For example, the polymer may contain 91% by weight or more, or 92% by weight or more, or 93% by weight or more, or 94% by weight or more, or 95% by weight or more, or 96% by weight or more, or 97% by weight or more, or 98% by weight or more, or 99% by weight or more, while 100% by weight or less, or 99% by weight or less, or 98% by weight or less, or 97% by weight or less, or 96% by weight or less, or 95% by weight or less, or 94% by weight or less, or 93% by weight or less, or 92% by weight or less, or 91% by weight or less of monomers having a log P of 1.0 or greater.

[0019] The polymer may include structural units from one or more of itaconic acid, fumaric acid, crotonic acid, acrylic acid, methacrylic acid, maleic acid, acryloxypropionic acid, citraconic acid, methyl acrylate, vinyl acetate, and combinations thereof.

[0020] Embodiments of the present disclosure provide polymers having a weight average molecular weight of 10,000 Daltons to 30,000 Daltons. For example, the polymer can have a molecular weight of 10,000 Daltons or greater, or 11,000 Daltons or greater, or 12,000 Daltons or greater, or 13,000 Daltons or greater, or 14,000 Daltons or greater, or 15,000 Daltons or greater, or 16,000 Daltons or greater, or 17,000 Daltons or greater, or 18,000 Daltons or greater, or 19,000 Daltons or greater, or 20,000 Daltons or greater, or 21,000 Daltons or greater, or 22,000 Daltons or greater, or 23,000 Daltons or greater, or 24,000 Daltons or greater, or 25,000 Daltons or greater, or 26,000 Daltons or greater, or 27,000 Daltons or greater, or 28,000 Daltons or greater, or 29,000 Daltons or greater, At the same time, 30,000 Daltons or less, or 29,000 Daltons or less, or 28,000 Daltons or less, or 27,000 Daltons or less, or 26,000 Daltons or less, or 25,000 Daltons or less, or 24,000 Daltons or less, or 23,000 Daltons or less, or 22,000 Daltons or less, or 21,000 Daltons or less, or 20,000 Daltons or less, or 19,000 Daltons or less, or 18,000 Daltons or less, or 17,000 Daltons or less, or 16,000 Daltons or less, or 15,000 Daltons or less, or 14,000 Daltons or less, or 13,000 Daltons or less, or 12,000 Daltons or less, or 11,000 Daltons or less. The weight average molecular weight of the polymer is determined using gel permeation chromatography.

[0021] The polymer can be prepared using known equipment, reaction components and reaction conditions. For example, the polymer can be prepared by known polymerization (e.g., solution polymerization). The solution polymerization of the monomer (i.e., the monomer discussed herein) can be carried out in, for example, a non-aqueous solvent. Suitable solvents include, but are not limited to, toluene, xylene, propylene glycol, methyl ethyl ketone, and combinations thereof. The solution polymerization may include a solvent-soluble initiator. Examples of initiators include, but are not limited to, t-butylperoctoate, t-butylperoctoate, AIBN, 2,2-azobis(2,4-dimethyl-valeronitrile), t-butyl peroxybenzoate, and combinations thereof. For example, based on the total weight of the monomers utilized in the solution polymerization, 0.01 wt % to 1.00 wt % of the initiator can be used. The solution polymerization may utilize a chain transfer agent. Examples of chain transfer agents include, but are not limited to, 2-mercaptoethanol, 3-methylmercaptopropionic acid, n-dodecyl mercaptan, t-dodecyl mercaptan, and combinations thereof. For example, 0.01 wt % to 5.00 wt % of a chain transfer agent can be used, based on the total weight of monomers utilized in the solution polymerization. The use of a mercaptan modifier can reduce the molecular weight of the polymer. Other known components can be used in solution polymerization; varying amounts of these other known components can be used for various applications.

[0022] The polymer can be prepared by known polymerization (e.g., emulsion polymerization). Emulsion polymerization can utilize a surfactant. Examples of surfactants include, but are not limited to, anionic surfactants such as sodium lauryl sulfate, sodium dodecylbenzene sulfonate, and ethoxylated sulfosuccinic acid. 10 ] alcohol half ester sodium and their combinations. For example, based on the total weight of the monomers used in the emulsion polymerization, 0.5 wt % to 6.0 wt % of the surfactant can be used. For example, the emulsion polymerization can utilize an initiator, such as a water-soluble initiator. Examples of initiators include, but are not limited to, alkali metal persulfates, ammonium persulfate, and combinations thereof. Based on the total weight of the monomers used in the emulsion polymerization, 0.01 wt % to 1.00 wt % of the initiator can be utilized. The emulsion polymerization can utilize chain transfer mercaptan. Examples of chain transfer mercaptan include, but are not limited to, 2-mercaptopropionic acid, 3-methylmercaptopropionic acid, alkyl mercaptan containing 4 to 20 carbon atoms, and combinations thereof. Based on the total weight of the monomers used in the emulsion polymerization, 0.01 wt % to 5.00 wt % of the chain transfer mercaptan can be utilized. The use of a mercaptan modifier can reduce the molecular weight of the polymer. Other known components can be used for emulsion polymerization; different amounts of these other known components can be used for various applications.

[0023] The polymers are commercially available under various trade names.

[0024] As described above, the monomer building blocks of the polymers described herein may undergo one or more reactions after the polymerization reaction (eg, a hydrolysis reaction). The hydrolysis reaction may include, for example, the hydrolysis of an ester to an acid or the ring opening of an anhydride to an acid.

[0025] The pest control composition disclosed herein comprises Bacillus thuringiensis. As defined herein, "Bacillus thuringiensis" is the spores and / or crystalline proteins of the Bacillus thuringiensis species, and includes all Bacillus thuringiensis subspecies that exhibit insecticidal properties. Examples of such subspecies include Kurstaki, Israelensis, and Aizawa. Bacillus thuringiensis can be added to the pest control composition as a solid or as part of a liquid formulation. The presence and subspecies of Bacillus thuringiensis are determined by random amplification polymorphic DNA analysis. A commercially available liquid formulation of Bacillus thuringiensis is THURICIDE available from Certis USA in Columbia, Maryland. TM Insecticide.

[0026] The pest control composition disclosed herein may include water. The pest control composition provided by one or more embodiments of the present disclosure is a solution, that is, the polymer and Bacillus thuringiensis are both water-soluble. Advantageously, the pest control composition disclosed herein can overcome many problems, such as using less components used together with emulsions and / or dispersions and / or redispersible polymers, such as surfactants. Different applications can use different amounts of water.

[0027] The pest control composition disclosed herein provided by one or more embodiments of the present disclosure may include additives. Examples of additives include viscosity modifiers, pH modifiers, herbicides, fungicides, and combinations thereof, etc. Different amounts of additives can be used for various applications.

[0028] The pest control composition disclosed herein may include 0.010 wt % to 5.000 wt % of humic acid based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water. All individual values ​​and subranges of 0.010 wt % to 5.000 wt % are included; for example, the pest control composition may include 0.010 wt %, 0.050 wt %, 0.100 wt % or 0.150 wt % to an upper limit of 5.000 wt %, 4.000 wt % or 3.500 wt % or 3.000 wt % of humic acid based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water.

[0029] The pest control composition disclosed herein may include 0.10 wt % to 20.00 wt % of the polymer based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water. All individual values ​​and subranges from 0.10 wt % to 20.00 wt % are included; for example, the pest control composition may include a lower limit of 0.10 wt %, 0.15 wt %, 0.20 wt %, 0.25 wt % or 0.30 wt % to an upper limit of 20.00 wt %, 15.00 wt %, 10.00 wt %, 9.00 wt % or 8.00 wt % of the polymer based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water.

[0030] The pest control composition disclosed herein may include 0.01% to 20.00% by weight of Bacillus thuringiensis based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water. All individual values ​​and subranges from 0.01% to 20.00% by weight are included; for example, the pest control composition may include a lower limit of 0.01%, 0.02%, 0.03%, 0.04% or 0.05% by weight to an upper limit of 20.00%, 15.00%, 10.00%, 7.50%, 5.00%, 4.75% or 4.50% by weight of Bacillus thuringiensis based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water.

[0031] The pest control composition disclosed herein may include 55.00 wt % to 99.79 wt % of water based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water. All individual values ​​and subranges from 55.00 wt % to 99.79 wt % are included; for example, the pest control composition may include a lower limit of 55.00 wt %, 60.00 wt %, 70.00 wt % or 80.00 wt % to an upper limit of 99.79 wt %, 99.00 wt % or 98.00 wt % or 95.00 wt % of water based on the total weight of the combination of humic acid, polymer, Bacillus thuringiensis and water.

[0032] The pest control composition disclosed herein can be formed using known equipment and methods. The components of the pest control composition can be combined (e.g., mixed) to form the pest control composition. For example, the components of the pest control composition can be added to a container and stirred therein. The components of the pest control composition can be combined in any order.

[0033] The pest control composition disclosed herein can be applied to plants, such as plant surfaces, to control pests. The pest control composition can be applied to plants using known equipment and methods. For example, the pest control composition can be sprayed, sprinkled and / or poured onto plants, among other applications. Different amounts of the pest control composition can be applied to plants for various applications.

[0034] Example

[0035] In the Examples, various terms and names of materials are used, and these terms and names include, for example, the following:

[0036] THURICIDE TM (liquid preparation, Bacillus thuringiensis, manufactured by Certis); Humic acid-1 (humic acid, solid, pH 9.3, measured with a 2.5 wt% Humic acid-1 solution having a phenolic group concentration of 0.375 mmol / g humic acid, sold under the trade name ACTIVEHUME TM Humic acid-1 (humic acid, obtained from ORGANOCAT, Louisville, Kentucky); Humic acid-2 (solid humic acid, obtained from SIGMA-ALDRICH); polyethylene oxide functionalized lignin. The phenolic concentration of Humic acid-1 was determined by preparing a humic acid solution (0.1 wt % humic acid and water). An aliquot of the humic acid solution was combined with the assay solution; the assay solution contained 4 wt % copper sulfate solution (excess Cu 2+ Salt; 7758-99-8; purchased from Thermo Fisher; product number 23224) and bicinchoninic acid (CAS 1245-13-2; obtained from Millipore Sigma; product number B9643). 2+ Reduced to Cu+ and forms a purple complex with bicinchoninic acid; by Varian Cary TM The concentration of the formed complex was determined colorimetrically by measuring the absorbance at 562 nm using a 50UV-Vis spectrophotometer. The concentration of the phenolic groups in humic acid-1 was determined from the concentration of Cu+-BCA using hydroquinone as a calibration standard; a linear calibration curve was plotted with the absorbance of hydroquinone on the y-axis against the number of nanomoles of phenolic groups on the x-axis, where y = 0.0104x, and R 2 = 0.9989. The phenolic concentration was determined as the number of nanomoles of phenol in the aliquot, which was then converted to millimoles per gram of humic acid using the dilution factor and the mass of humic acid.

[0037] Polymer-1 is formed as follows. A copolymer derived from diisobutylene and maleic anhydride is formed by solution polymerization. The weight % of polymer-1 formed from monomer structural units of diisobutylene is 45 weight % to 55 weight %, and the remainder is maleic anhydride. The polymer is hydrolyzed with aqueous ammonia to obtain polymer-1. Polymer-1 has a weight average molecular weight of about 16,500 Daltons.

[0038] Example 1, a pest control composition, was formed as follows. Polymer-1 was diluted with deionized water to obtain a solution (5 wt % aqueous solution of Polymer-1). The solution (1 mL), THURICIDE TM (7.38 wt % aqueous solution; 9.38 mL) and humic acid-1 (2.5 wt % aqueous solution; 0.26 mL) were added to a container and mixed with a magnetic stirring bar to obtain Example 1. The phenolic group concentration of the humic acid in Example 1 was 0.423077 mmol / g humic acid.

[0039] Example 2, a pest control composition, was formed as in Example 1, except that Humic Acid-2 (0.26 mL) was used instead of Humic Acid-1. The phenolic group concentration of the humic acid in Example 2 was 0.3942 mmol / g humic acid.

[0040] Comparative Example A was formed as Example 1, except that Polymer-1 was not utilized.

[0041] Comparative Example B was formed as in Example 1, except that Humic Acid-2 (0.26 mL) was utilized instead of Humic Acid-1, and Polymer-1 was not utilized.

[0042] Comparative Example C was formed as Example 1, except that polyethylene oxide functionalized lignin was utilized instead of Humic Acid-1, and Polymer-1 was not utilized.

[0043] Comparative Example D was formed as in Example 1, except that polyethylene oxide functionalized lignin was utilized instead of Humic Acid-1.

[0044] Bacillus thuringiensis activity was determined before and after exposure to light in Examples 1-2 and Comparative Examples AD as follows.

[0045] Using an automated pipette, 30 μL drops of each of Examples 1-2 and Comparative Examples AD were placed in a plastic petri dish; the drops were allowed to dry for approximately 1 hour.

[0046] For samples exposed to light, the dried droplets were exposed to 35 mW / cm 2 light for 2 hours.

[0047] The samples not exposed to light and the samples exposed to light were removed and plated. For extraction, each sample was placed 20 solution and incubate for about 12 hours. For plating, 0.1 wt % of The sample was diluted to the desired starting concentration with 20% solution, and then serially diluted at the appropriate concentration and evenly plated with 10 μL drops. The plate was then kept in an incubator at 30°C for about 12 hours. The number of colonies expressed as log colony forming units / mL was then counted, taking into account the dilution factor. The results are reported in Tables 1 to 3.

[0048] Table 1

[0049]

[0050] The data in Table 1 show that Example 1 has improved pest control agent activity after light exposure, i.e., 3.5 times, compared to Comparative Example A.

[0051] Table 2

[0052]

[0053] The data in Table 2 show that Example 2 has improved pest control agent activity after light exposure, i.e., 5.2 times, compared to Comparative Example B.

[0054] Table 3

[0055]

[0056] The data in Table 3 show that Comparative Example D has reduced pest control activity after light exposure, i.e., 1 / 10, compared to Comparative Example C. This reduced pest control activity after light exposure further illustrates the surprising synergistic effect between the components of the pest control composition disclosed herein.

Claims

1. A pest control composition comprising: Humic acid; A polymer, wherein the polymer has a weight average molecular weight of 10,000 to 30,000 Daltons and contains 20 to 100 weight percent of monomer structural units derived from monomers having a log P of 2.0 to 6.0; Bacillus thuringiensis; as well as water.

2. The pest control composition according to claim 1, wherein: The humic acid comprises 0.010 wt % to 5.000 wt % of the composition, based on the total weight of the combination of the humic acid, the polymer, the Bacillus thuringiensis, and the water.

3. The pest control composition according to any one of claims 1-2, wherein the polymer accounts for 0.10 wt% to 20.00 wt% of the composition, based on the total weight of the combination of the humic acid, the polymer, the Bacillus thuringiensis and the water.

4. The pest control composition according to any one of claims 1 to 3, wherein the polymer is a copolymer of diisobutylene and maleic anhydride. 5 . The pest control composition according to claim 1 , wherein the humic acid has a phenol group concentration of 0.35 mmol / g humic acid to 0.45 mmol / g humic acid.

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