Propagation substrate and preparation method thereof

Abstract

The invention discloses a propagation substrate and a preparation method thereof, and relates to the technical field of planting substrates, the propagation substrate comprises, by weight, 500-800 parts of engineering spoil, 100-300 parts of akadama soil, 100-200 parts of garden waste fermentation particles, 0.5-1 part of biochemical fulvic acid, 1-5 parts of compound amino acid, and 1-10 parts of an EM microbial agent. According to the technical scheme provided by the invention, the biochemical fulvic acid, the compound amino acid and the EM microbial agent are added, so that the group growth speed of the moss is accelerated, and the resistance to pathogenic microorganisms is improved, thereby greatly accelerating the propagation speed of the moss; meanwhile, waste engineering spoil and garden waste fermentation particles are utilized, and recycling of waste resources is achieved. By applying the substrate to the moss, the survival rate and the growth speed of the moss in the process are improved, so that the requirement for landscaping can be quickly met.

Description

Technical Field

[0001] The invention relates to the technical field of planting substrates, and in particular to a propagation substrate and a preparation method thereof. Background Art

[0002] Bryophytes are the second largest group of higher plants, with about 24,000 species worldwide. In my country, there are about 3,450 species in 125 families, 620 genera, etc., which are widely distributed in forests, deserts, polar regions and other ecosystems. Although bryophytes are tiny, they are of great significance to slope vegetation restoration. The settlement and expansion of bryophytes create conditions for the settlement and growth of vascular plants, and they are important primary producers and pioneer species in nature.

[0003] The cultivation medium plays an important role in the growth of moss plants. At present, there is little research on the moss propagation medium. The effects of both substrate culture and tissue culture are not ideal. The moss survival rate is low and the growth is slow, making it difficult to achieve production applications. Summary of the invention

[0004] The main purpose of the invention is to provide a propagation substrate and a preparation method thereof, aiming to improve the propagation speed and stress resistance of moss.

[0005] To achieve the above purpose, the propagation matrix proposed by the present invention includes the following components by weight: 500-800 parts of engineering waste soil, 100-300 parts of Akadama soil, 100-200 parts of garden waste fermentation particles, 0.5-1 part of biochemical fulvic acid, 1-5 parts of complex amino acids, and 1-10 parts of EM bacterial agent.

[0006] In one embodiment, the number of viable beneficial bacteria in each milliliter of the EM bacterial agent is ≥ 200 million CFU.

[0007] In one embodiment, the complex amino acids include glycine and methionine.

[0008] In one embodiment, the mass ratio of glycine to methionine in the composite amino acid is (1-2):1.

[0009] The present invention also provides a method for preparing the above-mentioned multiplication matrix, comprising the following steps:

[0010] S10, mixing engineering waste soil, Akadama soil and fermented garden waste particles to obtain a base;

[0011] S20, mixing the biochemical fulvic acid, the compound amino acid, the EM bacterial agent and water to obtain a mixed solution;

[0012] S30, spraying the mixed solution onto the substrate to obtain a propagation matrix.

[0013] In one embodiment, before step S10, the method further includes:

[0014] Step S101, crushing and sieving engineering waste soil, Akadama soil, garden waste fermentation particles, biochemical fulvic acid, compound amino acids and EM bacterial agent respectively.

[0015] In one embodiment, the mesh diameter of the sieve is 1.00-2.00 mm.

[0016] In one embodiment, the method for preparing biochemical fulvic acid comprises the following steps:

[0017] Step S1, drying and crushing rice straw to obtain straw powder;

[0018] Step S2, inoculating the bacterial liquid into the straw powder, adding water, and fermenting to obtain a fermentation liquid;

[0019] Step S3, filtering the fermentation liquid to obtain biochemical fulvic acid.

[0020] In one embodiment, the bacterial solution comprises Bacillus, Trichoderma and yeast; the mass ratio of Bacillus, Trichoderma and yeast is (2-2.5:2:1); and / or,

[0021] The inoculation amount of the bacterial solution is 10-15%.

[0022] In one embodiment, the fermentation temperature is 33-37°C; and / or,

[0023] The fermentation duration is 11-16 days.

[0024] In the propagation matrix provided in the technical solution of the present invention, the engineering waste provides basic minerals and soil structure, and the high clay content slows down water migration, improves water retention, provides necessary physical support for the growth of moss and creates a moist microenvironment. The garden waste fermentation particles are formed by crushing and screening urban garden waste after aerobic fermentation. The biochemical fulvic acid and composite amino acids are both biostimulants. The biochemical fulvic acid contains phenolic hydroxyl, carboxyl, alcohol hydroxyl and a small amount of enol, amino, quinone, methoxy and other functional groups, and has the function of controlling the opening and closing of plant stomata and enhancing the drought resistance of plants. At the same time, the biochemical fulvic acid is also a good propagation matrix for microorganisms. After it is applied to the soil, the beneficial bacteria can reproduce rapidly, and regulate the soil microbial community structure, improve the soil microbial diversity, and reduce the reproduction of miscellaneous bacteria and the occurrence of soil-borne diseases. In the technical solution provided by the present invention, engineering waste soil, Akadama soil and fermented particles of garden waste are mixed to obtain a substrate; the added biochemical fulvic acid, compound amino acid and EM bacterial agent promote the group growth rate of moss, improve the resistance to pathogenic microorganisms, thereby greatly accelerating the propagation speed of moss; at the same time, waste engineering waste soil and fermented particles of garden waste are utilized to achieve the recycling of waste resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0026] Figure 1 This is a physical diagram of the application of the multiplication matrix provided by the present invention.

[0027] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] The cultivation medium plays an important role in the growth of moss plants. At present, there is little research on the propagation medium. The effects of both matrix culture and tissue culture are not ideal. The survival rate of moss is low and the growth is slow, making it difficult to achieve production applications.

[0030] The present application proposes a propagation matrix, which includes the following components by weight: 500-800 parts of engineering waste soil, 100-300 parts of Akadama soil, 100-200 parts of garden waste fermentation particles, 0.5-1 part of biochemical fulvic acid, 1-5 parts of complex amino acids, and 1-10 parts of EM bacterial agent.

[0031] In the technical scheme of the present invention, the engineering waste soil provides basic minerals and soil structure, and the high clay content slows down water migration, improves water retention, provides necessary physical support for the growth of moss and creates a moist microenvironment. The red jade soil is formed by the accumulation of volcanic ash, is a highly permeable volcanic mud, dark red round particles, has no harmful bacteria, and has a slightly acidic pH value. Its shape is conducive to water storage and drainage, and has good air permeability, ensuring that the roots of moss can breathe smoothly. The garden waste fermented particles are conducive to the growth and reproduction of microorganisms. The biochemical fulvic acid and composite amino acids are both biostimulants. The biochemical fulvic acid contains phenolic hydroxyl, carboxyl, alcohol hydroxyl and a small amount of enol group, amino, quinone group, methoxyl and other functional groups, and has the function of controlling the opening and closing degree of plant stomata and enhancing the drought resistance of plants. At the same time, the biochemical fulvic acid is also a good breeding matrix for microorganisms. After it is applied to the soil, the beneficial bacteria can reproduce rapidly, and regulate the soil microbial community structure, improve the diversity of soil microorganisms, and reduce the reproduction of miscellaneous bacteria and the occurrence of soil-borne diseases. The technical solution provided by the present invention adds biochemical fulvic acid, compound amino acid and EM bacteria agent to promote the population growth rate of moss, improve the resistance to pathogenic microorganisms, thereby greatly accelerating the propagation speed of moss; at the same time, waste engineering waste soil and garden waste fermentation particles are utilized to achieve the recycling of waste resources. Under this component ratio, the moss has a high survival rate and grows fast.

[0032] It can be understood that in the embodiments of the present invention, the garden waste fermentation particles are made by crushing and screening urban garden waste after aerobic fermentation; preferably, the organic matter mass fraction (dry basis) of the garden waste fermentation particles is ≥40%; when the organic matter mass fraction (dry basis) of the garden waste fermentation particles reaches or exceeds 40%, it contains rich carbon sources, which is beneficial to the growth and reproduction of beneficial microorganisms and the improvement of soil nutrients.

[0033] In an embodiment of the present invention, the mass fraction of the functional active ingredients of the biochemical fulvic acid accounts for more than 30% of the whole. A high content of functional active ingredients means that it has stronger biological activity in promoting plant growth, increasing crop yields, and enhancing plant disease resistance. Functional active ingredients can promote the growth of beneficial microorganisms in the soil, improve soil aeration and water retention, and thus optimize the soil environment.

[0034] In an embodiment of the present invention, the total number of beneficial bacteria in each milliliter of the EM bacterial agent is ≥ 200 million CFU, including high concentrations of beneficial bacteria such as Bacillus, photosynthetic bacteria, and yeast. The high concentration of beneficial bacteria can improve the microbial environment of the soil, increase soil fertility, and improve the disease resistance of moss. The beneficial bacteria in the EM bacterial agent can decompose organic matter in the soil, fix nitrogen in the air, produce plant growth hormones, improve soil fertility, increase soil permeability and water retention, and thus improve soil structure.

[0035] In an embodiment of the present invention, the composite amino acid includes glycine and methionine. The glycine can be directly absorbed by the moss stem cuts to stimulate the synthesis of photosynthetic pigments; the methionine plays a core role in plant cell metabolism, and under appropriate conditions, it helps plant protein synthesis, demethylation reaction, polyamine and ethylene biosynthesis, and inhibits plant aging. After compounding, the composite amino acid serves as the main signal transduction substance for the physiological activities of the moss. Exogenous amino acid supplementation enables the moss to respond quickly to the environment and restore various life activities.

[0036] In an embodiment of the present invention, the mass ratio of glycine to methionine in the composite amino acid is (1-2): 1. Under this ratio, the composite amino acid can more effectively support the nutritional needs of moss.

[0037] The present invention also proposes a method for preparing the above-mentioned enrichment matrix. The enrichment matrix refers to the above-mentioned embodiment. Since the present application adopts all the technical solutions of all the above-mentioned embodiments, it at least has all the beneficial effects brought by the technical solutions of the above-mentioned embodiments, which will not be described one by one here. The preparation method of the enrichment matrix comprises the following steps:

[0038] S10, mixing engineering waste soil, Akadama soil and fermented garden waste particles to obtain a base;

[0039] S20, mixing the biochemical fulvic acid, the compound amino acid, the EM bacterial agent and water to obtain a mixed solution;

[0040] S30, spraying the mixed solution onto the substrate to obtain a propagation matrix.

[0041] In the technical solution of the present invention, in the mixed solution, the ratio of biochemical fulvic acid, compound amino acid and EM bacterial agent as a whole to water is 1: (100-500), that is, 250-8000 parts of water are added. The biochemical fulvic acid, compound amino acid and EM bacterial agent are diluted 100-500 times with water and evenly sprayed onto the substrate. The dilution ratio is based on the ability to evenly wet the moss propagation matrix. By utilizing engineering waste soil, red jade soil and garden waste fermentation particles, the waste treatment pressure is reduced and the recycling of resources is achieved. Since moss has no root system, the surface of the moss has a large number of pores and cells, which can absorb gases, dust and organic matter attached to its surface in the air. Spraying can better enable the moss to absorb nutrients.

[0042] In an embodiment of the present invention, before step S10, the method further includes:

[0043] Step S101, crushing and sieving the fermented particles of engineering waste soil, red jade soil, and garden waste. Crushing can significantly increase the surface area of ​​the material, increase the contact area between the plant stem and the biochemical fulvic acid, compound amino acid and EM bacterial agent, and is beneficial to the plant nutrient absorption. In addition, the gaps between the crushed matrix particles are increased, which is beneficial to the retention of air and water, and provides a good environment for plant growth.

[0044] In an embodiment of the present invention, the diameter of the sieve hole is 1.00-2.00 mm. Within this range, it is beneficial to increase the water-holding porosity and water-holding capacity of the substrate. Under semi-closed culture conditions, the air humidity in the culture environment can be ensured to reach 95% or above through evaporation, creating a microenvironment suitable for the growth of moss.

[0045] In the embodiment of the present invention, the engineering waste soil is clay obtained by sieving the original soil of the engineering project to remove stones, stone powder and sand, washing and sieving with reclaimed water, collecting slurry, naturally settling, and removing the supernatant. The physical properties of the clay after washing and precipitation are improved, and it is more suitable as a substrate for moss growth.

[0046] In an embodiment of the present invention, the mesh number of the sieve is 10-18. The engineering waste soil with this mesh number is more suitable as a substrate for moss growth.

[0047] In an embodiment of the present invention, the preparation process of the biochemical fulvic acid comprises the following steps: crushing and drying rice straw to obtain straw powder; inoculating bacterial liquid into the straw powder, diluting with a solvent and fermenting to obtain a fermentation liquid; filtering the fermentation liquid to remove residues to obtain the biochemical fulvic acid. Not only does it effectively utilize agricultural waste, but it also prepares biochemical fulvic acid with multiple application values, which is of great significance to promoting environmental protection and resource recycling.

[0048] In an embodiment of the present invention, the bacterial liquid includes Bacillus, Trichoderma and yeast; the mass ratio of the Bacillus, Trichoderma and yeast is (2-2.5):2:1. This mixing ratio is conducive to the synergistic effect between microorganisms and promotes the fermentation process. Bacillus, Trichoderma and yeast are rich in active functional groups and extracellular polysaccharides, which can enhance the mutual adhesion between cells and stimulate the division and growth of tissue cells. The prepared biochemical fulvic acid can directly pass through the cell membrane and act in the protoplast, promote the growth of the broken stem and the absorption of nutrients, and improve the survival rate of moss broken stem reproduction.

[0049] In the embodiment of the present invention, the inoculation amount of the bacterial liquid is 10-15%. The inoculation amount refers to the percentage of the bacterial liquid in the entire fermentation matrix. The inoculation amount of 10-15% can ensure that there are enough microbial populations to reproduce rapidly in the fermentation medium, thereby effectively starting the fermentation process.

[0050] In an embodiment of the present invention, the fermentation temperature is 33-37° C. At this temperature, the microorganisms in the fermentation process can be ensured to maintain a high activity, and it is also helpful to maintain the stability of the fermentation process and the quality of the product.

[0051] The fermentation time is 11-16 days. During the fermentation period of 11 to 16 days, the microorganisms have enough time to grow, reproduce and complete the metabolic process, the fermentation effect is the best, and the fulvic acid content is the highest.

[0052] Preferably, the preparation process of biochemical fulvic acid includes the following steps: rice straw is crushed into 5-6 cm small segments and dried at 85°C. After drying, it is crushed to 0.4 mm with a grinder to obtain straw powder. Bacillus, Trichoderma and yeast are prepared into a mixed bacterial solution in a ratio of 2:2:1, and inoculated into the straw powder at an inoculation amount of 10% (the inoculation amount refers to the percentage of bacterial solution in the entire fermentation matrix, inoculation amount = bacterial solution (mL) ÷ straw powder mass (g)), and the moisture content of the straw powder is adjusted to 65%, and fermented at 35°C for 15 days. The fermentation liquid is filtered to remove the residue, and the resulting product is biochemical fulvic acid.

[0053] Example 1

[0054] The present embodiment provides a propagation matrix, which includes the following components by weight: 800 parts of engineering waste soil (collected from excavated earth at a construction site on Guiwan 1st Road, Nanshan District, Shenzhen, with SiO2 50.48%, Al2O3 32.50%, Fe2O3 4.79%, pH 5.45, clay content 53.1%, and organic matter content 0.68 g / kg), 100 parts of red jade soil, 200 parts of garden waste fermentation particles, 1 part of biochemical fulvic acid, 5 parts of composite amino acids (glycine: methionine = 1:1), and EM bacterial agent (Foshan Anxiaoran Biotechnology Co., Ltd., effective viable bacteria count ≥ 2.4×108 CFU / mL, Bacillus ≥3.4×10 5 CFU / mL, photosynthetic bacteria ≥4.8×10 6 CFU / mL, yeast ≥1.4×10 7 CFU / mL) 5 copies.

[0055] Example 2

[0056] The present embodiment provides a propagation matrix, which includes the following components by weight: 500 parts of engineering waste soil (collected from excavated earth at a construction site on Guiwan Road, Nanshan District, Shenzhen, with SiO2 50.48%, Al2O3 32.50%, Fe2O3 4.79%, pH 5.45, clay content 53.1%, and organic matter content 0.68 g / kg), 300 parts of red jade soil, 150 parts of garden waste fermentation particles, 0.5 parts of biochemical fulvic acid, 1 part of composite amino acids (glycine: methionine = 1:1), and EM bacterial agent (Foshan Anxiaoran Biotechnology Co., Ltd., effective viable count ≥ 2.4×10 8 CFU / mL, Bacillus ≥3.4×10 5 100 million CFU / mL, photosynthetic bacteria ≥4.8×10 6 CFU / mL, yeast ≥1.4×10 7 CFU / mL) 10 copies.

[0057] Example 3

[0058] The present embodiment provides a propagation matrix, which includes the following components by weight: 600 parts of engineering waste soil (collected from excavated earth at a construction site on Guiwan 1st Road, Nanshan District, Shenzhen, with SiO2 50.48%, Al2O3 32.50%, Fe2O3 4.79% pH 5.45, clay content 53.1%, and organic matter content 0.68 g / kg), 200 parts of red jade soil, 100 parts of garden waste fermentation particles, 1 part of biochemical fulvic acid, 1 part of composite amino acids (glycine: methionine = 1:1), and EM bacterial agent (Foshan Anxiaoran Biotechnology Co., Ltd., effective viable bacteria count ≥ 2.4×10 8 CFU / mL, Bacillus ≥3.4×10 5 100 million CFU / mL, photosynthetic bacteria ≥4.8×10 6 CFU / mL, yeast ≥1.4×10 7 CFU / mL) 10 copies.

[0059] Comparative Example 1

[0060] This comparative example provides a propagation matrix, which only includes 800 parts of engineering waste soil.

[0061] Comparative Example 2

[0062] This comparative example provides a propagation matrix, which is different from Example 3 in that it does not include biochemical fulvic acid and complex amino acids.

[0063] Comparative Example 3

[0064] This comparative example provides a propagation matrix, which is different from Example 3 in that it does not include EM bacterial agent.

[0065] Comparative Example 4

[0066] This comparative example provides a propagation substrate, which is different from Example 3 in that it does not include biochemical fulvic acid.

[0067] Comparative Example 5

[0068] This comparative example provides a multiplication matrix, which is different from Example 3 in that it does not include complex amino acids.

[0069] Comparative Example 6

[0070] The comparative example provides a propagation matrix, which includes the following components by weight: 400 parts of engineering waste soil (collected from excavated earth at a construction site on Guiwan 1st Road, Nanshan District, Shenzhen, with SiO2 50.48%, Al2O3 32.50%, Fe2O3 4.79%, pH 5.45, clay content 53.1%, and organic matter content 0.68 g / kg), 200 parts of red jade soil, 100 parts of garden waste fermentation particles, 1 part of biochemical fulvic acid, 3 parts of composite amino acids (glycine: methionine = 1:1), and 5 parts of EM bacterial agent (Foshan Anxiaoran Biotechnology Co., Ltd., with an effective viable count of ≥2.4×10 8 CFU / mL, Bacillus ≥3.4×10 5 100 million CFU / mL, photosynthetic bacteria ≥4.8×10 6 CFU / mL, yeast ≥1.4×10 7 CFU / mL).

[0071] Comparative Example 7

[0072] The comparative example provides a propagation matrix, which includes the following components by weight: 900 parts of engineering waste soil (collected from excavated earth at a construction site on Guiwan 1st Road, Nanshan District, Shenzhen, with SiO2 50.48%, Al2O3 32.50%, Fe2O3 4.79%, pH 5.45, clay content 53.1%, and organic matter content 0.68 g / kg), 200 parts of red jade soil, 100 parts of garden waste fermentation particles, 1 part of biochemical fulvic acid, 3 parts of composite amino acids (glycine: methionine = 1:1), and EM bacterial agent (Foshan Anxiaoran Biotechnology Co., Ltd., effective viable bacteria ≥ 2.4×10 8CFU / mL, Bacillus ≥3.4×10 5 100 million CFU / mL, photosynthetic bacteria ≥4.8×10 6 CFU / mL, yeast ≥1.4×10 7 CFU / mL) 5 copies.

[0073] Performance Testing

[0074] Light incubator test

[0075] Taking the large gray moss as an example, the stem cutting propagation method was adopted, and the natural growth environment of the large gray moss was simulated and cultured in an artificial climate incubator. The conditions of the artificial climate incubator were set as follows: humidity 85%, temperature 25°C, light 4800Lx, and light-dark cycle 14h / 10h (14 hours of light and 10 hours of darkness). The culture device was a 9cm culture dish, and the sowing amount was 20g / m 2 (dry weight), stem length is 0.5-0.7cm. Sow evenly on the prepared substrate, cover with a transparent lid with holes after moistening, and place in an artificial climate incubator. Keep moist but not dripping during the incubation period. Harvest after 60 days, and measure the growth and physiological indicators of moss. Please refer to Figure 1 From Example 1 (i.e., "Example 1" in the figure) - Example 3 (i.e., "Example 3" in the figure) and Comparative Examples 1 to 7, it can be seen that the large gray moss in the three examples grows vigorously, and the antibacterial and stress resistance capabilities are greatly improved compared with Comparative Examples 1 to 7.

[0076] Number of branches: 5 points were evenly selected in the diameter direction of the culture dish, and a plastic sample frame divided into 9×1cm×1cm by 4 cross lines was placed. Five squares were randomly selected from it. The number of branches was determined by the plant body protruding ≥2mm out of the pseudo-semen as the standard for new branch germination. The number of new branches for each treatment was measured at 60 days.

[0077] Coverage value: A 2 mm × 2 mm grid was spread flat in the culture container, and the grayscale values ​​of the moss-covered grid and the uncovered and partially covered grid in the image were calculated using the vertical photography method combined with Image J software and binarized. The ratio of the covered area to the container area was taken as the coverage value (the calculation formula is as follows).

[0078] C(%)=(a / s)×100%;

[0079] Among them, C is the coverage value, a is the coverage area, and s is the container area.

[0080] The chlorophyll content was determined by acetone extraction method, the soluble sugar content was determined by anthrone colorimetry, and MDA was determined by TBA colorimetry.

[0081] Table 1 Moss growth and physiological indicators in different embodiments and comparative examples

[0082]

[0083] As shown in Table 1, the number of moss branches, coverage, chlorophyll content, and soluble sugar content in Example 3 increased by 15.61%, 19.76%, 17.11%, and 10.53%, respectively, compared with Comparative Example 2, and the MDA content decreased by 22.35%, indicating that biochemical fulvic acid and composite amino acids have a growth-promoting and stress-resistant effect on moss. The number of moss branches, coverage, chlorophyll content, and soluble sugar content in Example 3 increased by 35.34%, 36.79%, 8.54%, and 16.67%, respectively, compared with Comparative Example 3, and the MDA content decreased by 24.35%, among which the number of branches increased most significantly, indicating that EM bacteria have a promoting effect on the germination of new branches of moss. Compared with Comparative Example 4, the number of moss branches, coverage, chlorophyll content and soluble sugar content in Example 3 increased by 28.25%, 25.73%, 4.71% and 9.09%, respectively, and the MDA content decreased by 18.65%. The large increase in the number of branches and coverage indicates that biochemical fulvic acid has a stimulating effect on the growth and propagation of moss. Compared with Comparative Example 5, the number of moss branches, coverage, chlorophyll content and soluble sugar content in Example 3 increased significantly by 21.86%, 20.48%, 9.88% and 12.00%, respectively, and the MDA content decreased by 17.34%, indicating that the composite amino acid also has a stimulating effect on the growth and propagation of moss. Compared with Example 3, the number of moss branches, coverage and soluble sugar content in Comparative Example 6 were reduced by 6.39%, 3.47% and 4.76%, respectively, and the MDA content increased by 17.07%, indicating that too little engineering spoil is not conducive to the germination and expansion of new branches of mosses. Compared with Example 3, the number of moss branches, coverage, chlorophyll content and soluble sugar content in Comparative Example 7 were reduced by 13.68%, 11.06%, 5.62% and 8.33%, respectively, and the MDA content increased by 13.66%, indicating that too much engineering spoil is not conducive to the germination and expansion of new branches of mosses.

[0084] The above description is only an exemplary embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the technical concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A propagation matrix, characterized in that The invention comprises the following components by weight: 500-800 parts of engineering waste soil, 100-300 parts of Akadama soil, 100-200 parts of fermented garden waste particles, 0.5-1 parts of biochemical fulvic acid, 1-5 parts of complex amino acids and 1-10 parts of EM bacterial agent.

2. The multiplication matrix according to claim 1, characterized in that The number of viable beneficial bacteria in each milliliter of the EM bacterial agent is greater than or equal to 200 million CFU.

3. The multiplication matrix according to claim 1, characterized in that The complex amino acids include glycine and methionine.

4. The multiplication matrix according to claim 3, characterized in that The mass ratio of glycine to methionine in the composite amino acid is (1-2):

1.

5. A method for preparing a multiplication matrix according to any one of claims 1 to 4, characterized in that: The following steps are involved: S10, mixing engineering waste soil, Akadama soil and fermented garden waste particles to obtain a base; S20, mixing the biochemical fulvic acid, the compound amino acid, the EM bacterial agent and water to obtain a mixed solution; S30, spraying the mixed solution onto the substrate to obtain a propagation matrix.

6. The method for preparing the multiplication matrix according to claim 5, characterized in that: Before step S10, the method further includes: Step S101, crushing and sieving engineering waste soil, Akadama soil, garden waste fermentation particles, biochemical fulvic acid, compound amino acids and EM bacterial agent respectively.

7. The method for preparing the multiplication matrix according to claim 6, characterized in that: The diameter of the sieve holes is 1.00-2.00 mm.

8. The method for preparing the multiplication matrix according to claim 5, characterized in that: The preparation method of the biochemical fulvic acid comprises the following steps: Step S1, drying and crushing rice straw to obtain straw powder; Step S2, inoculating the bacterial liquid into the straw powder, adding water, and fermenting to obtain a fermentation liquid; Step S3, filtering the fermentation liquid to obtain biochemical fulvic acid.

9. The method for preparing a multiplication matrix according to claim 8, characterized in that: In step S2: The bacterial liquid comprises Bacillus, Trichoderma and yeast; wherein the mass ratio of the Bacillus, Trichoderma and yeast is (2-2.5):2:1; and / or, The inoculation amount of the bacterial solution is 10-15%.

10. The method for preparing a multiplication matrix according to claim 8, characterized in that: In step S3: The fermentation temperature is 33-37°C; and / or, The fermentation time is 11-16 days.

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