A surgical knife for removing bumblebee spermatheca and its preparation process

Abstract

The present invention relates to the technical field of insect surgical instruments, and provides a scalpel for removing the spermatheca of bumblebees and a preparation process thereof. The scalpel comprises a knife body of a columnar structure; a bevel is provided at the tip of the knife body to form a beveled tip structure, and a capsule-taking groove is provided at the beveled section of the knife body; an auxiliary groove is provided at the tail of the knife body to cooperate with the beveled tip to remove the spermatheca. The design of the scalpel of the present invention fully considers the deep hiding position of the spermatheca of female bumblebees and the complex structure around it; the bevel of the knife tip and the capsule-taking groove are designed according to the characteristics of the spermatheca, so that the scalpel can more accurately locate and remove the spermatheca, greatly improving the accuracy of the operation and reducing the damage caused by misoperation.

Description

Technical Field

[0001] The invention relates to the technical field of insect surgical instruments, and in particular to a scalpel for removing bumblebee spermatheca and a preparation process thereof. Background Art

[0002] In entomological research, removing specific tissues through delicate surgical operations to explore their functions is a classic and effective method commonly used to solve scientific problems. For example, in verifying the function of the pharyngeal body of honey bees, scientists successfully verified that this tissue plays a key role in regulating the secretion of juvenile hormone by removing its pharyngeal body. The success of these methods provides an important reference for studying the functions of other insect tissues, and there are also successful cases in social insects.

[0003] However, it is difficult to effectively remove the spermatocysts of some insects with large structural differences and relatively complex structures using existing methods. For example, due to the special location of the spermatocysts of female social bumblebees, which are deeply hidden in the body and have complex surrounding structures, it is difficult to accurately remove them using existing insect needles. Therefore, it is urgent to develop a scalpel that can be used to remove the spermatocysts of female bumblebees, so as to effectively remove the spermatocysts and ensure the survival of the bumblebees. Summary of the invention

[0004] The invention provides a scalpel for removing the spermatocyst of bumblebees and a preparation process thereof, so as to solve the defect that the scalpel in the prior art is difficult to effectively remove the spermatocyst of bumblebees.

[0005] The first aspect of the present invention provides a scalpel for removing the spermatheca of bumblebees, comprising: a knife body with a columnar structure; a knife tip portion of the knife body is provided with an inclined surface to form a bevel tip structure, and the knife body located at the inclined surface section is provided with a spermatheca removal groove; the knife tail portion of the knife body is provided with an auxiliary groove for cooperating with the bevel tip to remove the spermatheca.

[0006] According to the surgical knife for removing bumblebee spermatheca provided by the present invention, the end of the tail of the knife has a hemispherical structure.

[0007] According to the surgical knife for removing bumblebee spermatheca provided by the present invention, a cross-cutting plane is further provided on the knife body, and the cross-cutting plane is located at one end of the knife body where the auxiliary groove is located.

[0008] According to the surgical knife for removing the spermatheca of bumblebees provided by the present invention, the spermatheca removal groove extends along the axial direction of the knife body.

[0009] According to the surgical knife for removing the bumblebee spermatheca provided by the present invention, the diameter of the spermatheca removal groove is 0.22-0.5 mm, and the depth of the spermatheca removal groove is 2 mm.

[0010] According to the surgical knife for removing bumblebee spermatheca provided by the present invention, the center line of the auxiliary groove is perpendicular to the axial direction of the knife body, and the depth of the auxiliary groove is smaller than the diameter of the knife body.

[0011] According to the surgical knife for removing the bumblebee spermatheca provided by the present invention, the diameter of the auxiliary groove is 0.22-0.5 mm, and the depth of the auxiliary groove is 0.2 mm.

[0012] According to the scalpel for removing the bumblebee spermatheca provided by the present invention, the inclination angle of the inclined surface is 30-35°.

[0013] According to the surgical knife for removing the bumblebee spermatheca provided by the present invention, the knife body is a cylindrical structure.

[0014] The second aspect of the present invention provides a process for preparing a surgical knife for removing a bumblebee spermatheca as described in any one of the above items, comprising the following steps:

[0015] S10, selecting a columnar material of 304 stainless steel as the raw material of the knife body and cutting it into shape, retaining a processing allowance to form a knife blank, and performing preliminary grinding and decontamination on the surface of the knife blank to form a knife body;

[0016] S20, using a processing device to process a tip hole at the tip of the knife body, and using wire cutting to perform bevel cutting at the tip position to form a beveled tip structure and a capsule removal groove at the tip position;

[0017] S30, using a processing device to process a hemispherical structure at the tail of the cutter body, and to process an auxiliary groove at the tail of the cutter body;

[0018] S40, finely polishing the entire blade body, and inspecting it after polishing to obtain a qualified surgical knife.

[0019] The present invention provides a scalpel for removing the spermatheca of bumblebees and a preparation process thereof. By arranging a spermatheca removal groove at the tip of a knife body and an auxiliary groove at the tail of a knife body, when removing the spermatheca, two knife bodies are used, one knife body uses the knife tip to remove the spermatheca, and the other knife body uses the knife tail to assist in removing and disconnecting the spermatheca tube, thereby achieving effective removal of the spermatheca and ensuring the survival of the bumblebee. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the overall structure of the scalpel provided by the present invention.

[0022] Figure 2 The figure is a schematic structural diagram of the tail portion of the surgical knife provided by the present invention.

[0023] Figure 3 The present invention is a schematic flow chart of the process for preparing the surgical knife.

[0024] Figure 4 It is a schematic diagram of the reproductive cavity without removing the spermatheca cavity in an embodiment of the minimally invasive surgical method provided by the present invention.

[0025] Figure 5 It is a schematic diagram of the reproductive cavity in which the spermatheca cavity is successfully removed by using a scalpel in the minimally invasive surgical method provided by the present invention.

[0026] Reference numerals:

[0027] 1. knife body; 11. knife tip; 111. capsule removal groove; 112. bevel tip structure; 12. knife tail; 121. auxiliary groove; 122. platform. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of explaining the embodiments of the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limitations on the embodiments of the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0030] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0031] In the embodiments of the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0032] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms are not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0033] In some related technologies, insect pins can be used to remove insect tissues, so as to facilitate functional verification and experimental exploration. For example, in 1975, Dr. ERIC J. VILLAVASO of the U.S. Department of Agriculture Research Bureau published an important study. He cleverly used insect pins as tools to remove the spermathecae of cotton boll weevils, thereby deeply exploring the specific functions of spermathecae in the reproductive process. However, due to the limitations of its structure, the existing insect pins are difficult to effectively remove the spermathecae of bumblebees. Therefore, the present invention provides a scalpel that can remove the spermathecae of bumblebees.

[0034] Regarding the problems in related technologies, such as Figure 1 , Figure 2As shown, the present invention provides a surgical knife for removing the spermatheca of bumblebees, comprising a blade body 1 of a columnar structure; a blade tip 11 of the blade body 1 is provided with an inclined surface to form an oblique cutting tip structure 112, and a capsule taking groove 111 is provided in the blade body 1 at the inclined surface section; an auxiliary groove 121 is provided in the blade tail 12 of the blade body 1 to cooperate with the oblique cutting tip to remove the spermatheca. In the process of removing the spermatheca, it is necessary to puncture the bumblebee and find the position of the spermatheca to remove it. In this embodiment, the oblique cutting tip structure enables this end of the blade body 1 to have a puncture function, so that the bumblebee can be punctured and the spermatheca can be removed at the designated position.

[0035] The specific minimally invasive surgical procedure for removing the spermatheca is as follows: two scalpels are selected, and the tip 11 (i.e., the end with the beveled tip) of one of the scalpels is used to pierce the body surface of the bumblebee and catch the spermatheca, at which point the spermatheca is partially located in the spermatheca removal groove 111 of the tip of the scalpel, and then the auxiliary groove 121 of the tail 12 (the end with the auxiliary groove 121) of the other scalpel is used to perform auxiliary cutting near the tip 11, thereby removing the spermatheca. Specifically, the following steps are included:

[0036] Step S1, selecting a newly emerged female bumblebee whose spermatheca needs to be removed.

[0037] Step S2: using an insect artificial insemination instrument to anesthetize the bumblebee with carbon dioxide, opening the queen bee's reproductive organs under the insect artificial insemination instrument to expose the reproductive cavity of the female bee, and a transparent spermatheca can be seen on the right side of the reproductive opening.

[0038] Step S3, at a position of about 1-1.5mm on the left side of the spermathecae (near the base of the stinger), use the right hand to operate the bumblebee scalpel, and make the angle between the longitudinal axis of the scalpel and the vertical line about 35 degrees, and use the tip 11 to gently insert it to a depth of about 1-2mm (avoid directly piercing the spermathecae), so as to cause the reproductive cavity membrane to be cut open, clearly see and make the spermathecae fall smoothly into the spermathecae-taking groove 111 of the bumblebee scalpel, and then gently withdraw the scalpel (roughly withdraw about 1mm, and ensure that the spermathecae is also smoothly taken out of the bumblebee body). Adjust the angle and clearly see the spermathecae tube, then use the left hand to hold another scalpel to gently press the spermathecae tube, and move the left hand scalpel left and right to disconnect the spermathecae tube, and after disconnection, the two scalpels can be completely withdrawn, and the spermathecae are taken out with the bumblebee scalpel, and the female spermathecae of the bumblebee can be removed.

[0039] The above-mentioned minimally invasive surgery process is described in detail below through specific embodiments.

[0040] Examples of minimally invasive surgical methods:

[0041] Select the newly emerged queen bee of ground bumblebee. Use the insect artificial insemination instrument to anesthetize the queen bee with carbon dioxide. Open the queen bee's reproductive organs under the insect artificial insemination instrument to expose the queen bee's reproductive cavity. You can see the following on the right side of the reproductive opening: Figure 4 As shown (circled part in the figure), the transparent spermatocyst of the reproductive cavity of the bumblebee queen spermatocyst has not been removed. At a position of about 1.5mm on the left side of the spermatocyst (near the base of the stinger), use the bumblebee scalpel held in the right hand, with the angle between the longitudinal axis of the bumblebee scalpel and the vertical line about 35 degrees, and gently insert the tip 11 of the knife to a depth of about 2mm (avoid directly piercing the spermatocyst), so as to cause the reproductive cavity membrane to be cut open, so that the spermatocyst can smoothly fall into the spermatocyst removal groove 111 of the bumblebee scalpel, and gently withdraw the scalpel (to ensure that the spermatocyst is also smoothly taken out). At this time, the spermatocyst tube can be clearly seen and the withdrawal can be stopped at the same time. Then use the auxiliary groove 121 of another scalpel held in the left hand to gently press the spermatocyst, and move the scalpel left and right to cut the spermatocyst tube. After cutting the spermatocyst tube, the spermatocyst can be separated from the female reproductive cavity, and the operation is completed as shown below Figure 5 The reproductive cavity of the bumblebee queen's spermatheca was successfully removed (circled part in the picture).

[0042] Of course, in other embodiments, the position and depth of the cutting of the knife tip can be adjusted for different species of bumblebees according to the specific structure. For example, for the queen bee of Lanzhou bumblebee, the cutting depth can be 2 mm at a position about 1.5 mm to the left of the spermathecae (near the base of the stinger). For the queen bee of dense forest bumblebee, the cutting depth can be 1.5 mm at a position about 1.5 mm to the left of the spermathecae (near the base of the stinger). That is, those skilled in the art can make adjustments according to the specific mechanism to achieve accurate removal of the spermathecae using a scalpel.

[0043] Specifically, the above-mentioned minimally invasive surgery combines the tip and tail of the scalpel in this embodiment, so that it can remove the spermatocyst of the female bumblebee in a simple, accurate, effective and less harmful way to the health of the bumblebee. It provides researchers with a new experimental method, which helps to further explore the reproductive mechanism and physiological characteristics of bumblebees. Further research on the function of the spermatocyst of the female bumblebee is of great significance for understanding its reproductive mechanism. This technology also provides a reference for the study of the reproductive system of other insects, and provides a solution that can effectively achieve tissue extraction, which has broad development prospects.

[0044] It is understandable that the design of the scalpel fully considers the deep location of the female bumblebee spermatheca and the complex surrounding structure; the inclined surface of the blade tip and the spermatheca removal groove 111 are designed according to the characteristics of the spermatheca, so that the scalpel can more accurately locate and remove the spermatheca, greatly improving the accuracy of the operation and reducing the damage caused by misoperation. And by setting the blade tip and blade tail structures at both ends of the same column, it is convenient for operators to replace and facilitate production.

[0045] In the specific configuration, the blade body 1 is a cylindrical column structure as a whole. The cylindrical structure is conducive to overall molding, which can reduce the overall preparation difficulty and preparation process, thereby reducing the preparation cost and improving the manufacturing efficiency of mass production.

[0046] In some embodiments, the end of the blade tail 12 has a hemispherical structure. That is, the end of the blade tail 12 is processed to form a hemispherical structure, which can effectively prevent the cutting of other parts of the bumblebee during the auxiliary cutting process, and effectively improve the survival rate of the bumblebee after removal.

[0047] It is understandable that during the process of removing the spermatheca, the auxiliary groove 121 of the knife tail 12 is needed to assist the knife tip to disconnect the spermatheca tube and remove the spermatheca. In this embodiment, the end of the knife tail is processed into a hemispherical structure to avoid damage to irrelevant tissues of the bumblebee during the auxiliary process.

[0048] According to an embodiment provided by the present invention, a platform portion 122 is provided on a section of the blade body 1 near the auxiliary groove 121, and the platform portion 122 extends to the end of the blade tail portion 12. The arrangement of the platform portion 122 can facilitate the auxiliary fit between the tail portion and the blade tip, so as to facilitate the auxiliary cutting operation.

[0049] Specifically, the platform portion 122 is a horizontal platform, and the platform portion 122 makes a section of the top surface of the tail of the blade body 1 a horizontal plane structure, which is conducive to the inclined surface fitting with the blade tip position during the auxiliary process, thereby facilitating the blade tip to disconnect and cut, thereby achieving rapid removal of the spermatheca.

[0050] In some embodiments, the sac-taking groove 111 extends along the axial direction of the blade body 1. The sac-taking groove 111 is located on the blade body 1 where the bevel section is located. By extending the sac-taking groove 111 along the axial direction of the blade body 1, it is convenient for processing, and the sperm sac can be temporarily stored in the sac-taking groove 111. Since the entire length of the sac-taking groove 111 is arranged in the blade body 1 section corresponding to the blade tip bevel structure, no other tissue will remain in the sac-taking groove 111 after the sperm sac is subsequently taken out.

[0051] In the specific setting, the diameter of the sac-taking groove 111 is 0.22-0.5 mm, and the depth of the sac-taking groove 111 is 2 mm. By limiting the size of the sac-taking groove 111, it can meet the needs of extracting the spermatophore of most bumblebees, and by limiting the depth of the sac-taking groove, no residual tissue will remain in the sac-taking groove 111 during the process of extracting the spermatophore, which is convenient for cleaning the sac-taking groove 111. The depth of the sac-taking groove 111 is the depth of the axial opening of the blade body 1.

[0052] It is understandable that the sac removal groove 111 is shallow and is only located in the inclined surface area, while the entire part of the blade body 1 is a solid structure, which ensures that no excess tissue remains after the sperm sac is removed, making it easy to clean.

[0053] In some embodiments, the center line of the auxiliary groove 121 is perpendicular to the axis direction of the blade body 1, and the depth of the auxiliary groove 121 is less than the diameter of the blade body 1. The auxiliary groove 121 is used to cooperate with the blade tip to quickly cut the spermatheca tissue when removing the spermatheca. The vertical setting of the auxiliary groove 121 is conducive to the processing of the auxiliary groove 121, reducing the processing difficulty.

[0054] For example, a high-speed steel twist drill with a diameter of 0.21-0.49 mm can be used for drilling, and the hole of the drilled hole is perpendicular to the blade body 1. When the platform portion 122 is cut, the top of the auxiliary groove 121 is an open structure, which can be matched with the sac-taking groove 111 at the tip of the blade, so that the tip of the blade can be used for cutting when removing the sperm sac. That is, when performing the sac-taking operation, the auxiliary groove 121 can prevent the sperm sac located inside the sac-taking groove 111 from falling, thereby assisting the tip of the blade in the cutting operation.

[0055] In specific configuration, the diameter of the auxiliary groove 121 is 0.22-0.5 mm, and the depth of the auxiliary groove 121 is 0.2 mm. The auxiliary groove 121 has only one opening in the direction perpendicular to the blade body 1, that is, the auxiliary groove 121 does not radially penetrate the radial direction of the blade body 1, so that the auxiliary blade tip can perform cutting operations during operation.

[0056] According to some embodiments provided by the present invention, the inclined surface has an angle of 30-35°. The angle between the inclined surface and the axis of the blade body 1 is the inclined angle, and by limiting the inclined angle, the blade tip can have a sharper end, which can achieve rapid puncture and cutting.

[0057] like Figure 3 As shown, the second aspect of the present invention provides a process for preparing a scalpel for removing bumblebee spermatheca provided in any one of the above items, comprising the following steps:

[0058] Step S10, selecting a columnar material of 304 stainless steel as the raw material of the knife body 1 and cutting and shaping it, retaining a processing allowance to form a knife blank, and preliminarily grinding and cleaning the surface of the knife blank to form the knife body 1.

[0059] Specifically, a round bar made of 304 stainless steel is preliminarily cut and formed, and a processing allowance is retained. The surface of the cut knife blank is preliminarily polished, and surface cleaning treatments such as degreasing and decontamination are performed.

[0060] Step S20, using a processing device to process a tip hole on the tip portion 11 of the blade body 1, and using wire cutting to perform bevel cutting at the tip position to form a beveled tip structure 112 and a capsule removal groove 111 at the tip position.

[0061] Specifically, the control system of the CNC machine tool is used to accurately set the processing path, depth, speed and other parameters to ensure that the diameter of the capsule removal groove 111 at the tip of the knife is between 0.25 and 0.5 mm, the depth is 2 mm, and it is parallel to the axis of the knife body 1, and the center of the capsule removal groove 111 coincides with the center of the knife body 1. After the setting is completed, the capsule removal groove 111 is processed by the CNC machine tool to obtain the capsule removal groove 111.

[0062] Step S30: using a processing device to process a hemispherical structure on the blade tail portion 12 of the blade body 1, and to process an auxiliary groove 121 on the blade tail portion 12 of the blade blank.

[0063] Specifically, the control system of the CNC machine tool is used to accurately set parameters such as the processing path, depth and speed, and a high-speed steel twist drill with a diameter of 0.21~0.49mm is used for drilling, and the drilled hole is made perpendicular to the tool body 1, so that the diameter of the auxiliary groove 121 at the tool tail is 0.25~0.5mm, and the drilling depth is 0.2mm.

[0064] Step S40: finely polish the entire blade body 1, and inspect it after polishing to obtain a qualified surgical knife.

[0065] Specifically, after polishing, the scalpel is thoroughly cleaned and inspected to see if all indicators of the scalpel meet the design requirements. If they meet the design requirements, they are qualified scalpels.

[0066] The above-mentioned preparation process is described below through specific examples.

[0067] Embodiment 1:

[0068] According to the spermathecae data of the queen bumblebee, a scalpel for removing the spermathecae of female bumblebees is made:

[0069] Step 1: Cutting and forming: Use 304 stainless steel round bars with a diameter of 0.32mm as raw materials, cut the round bars into a length of 5cm, and retain a processing allowance of 0.5mm. Grind the surface of the cut knife blank, and perform surface cleaning treatments such as degreasing and decontamination.

[0070] Step 2, groove processing: use the control system of the CNC machine tool to accurately set the processing path, depth, speed and other parameters, and process the tip of the tool to form a groove with a diameter of 0.26mm and a depth of 2mm as the capsule removal groove 111, which is parallel to the axis of the tool body 1, and the center of the capsule removal groove 111 coincides with the center of the tool body 1; the diameter of the hemispherical auxiliary groove 121 at the tail of the tool is 0.26mm, and the drilling depth is 0.2mm.

[0071] Step 3, tool tip bevel processing: high-precision wire cutting technology is used to accurately process the bevel tool tip on the tool tip portion 11 of the tool blank, and the feed speed and cutting depth of the wire cutting are controlled to process the bevel tool tip with an inclination angle of 30° and a length of 2 mm.

[0072] Step 4: Processing of the hemisphere at the end of the cutter: The control system of the CNC machine tool accurately sets the processing path, depth, speed and other parameters to process a hemisphere that is consistent with the diameter of the round rod.

[0073] Step 5, overall surface processing of the blade body 1: Polishing preparation: prepare polishing wheels and polishing pastes of different grits, as well as necessary polishing equipment and fixtures; Rough polishing: Use a coarser-grained polishing wheel to roughly polish the entire scalpel to remove larger surface roughness; Medium polishing: Use a medium-grained polishing wheel and polishing paste to further refine the roughness of the scalpel surface; Fine polishing: Use a fine-grained polishing wheel and polishing paste for fine polishing until the surface of the scalpel reaches the required smoothness.

[0074] Step 6: Cleaning and inspection: After polishing, clean thoroughly and inspect whether the various indicators of the scalpel meet the design requirements.

[0075] Embodiment 2:

[0076] According to the spermathecae data of Lanzhou bumblebee queen, a scalpel for removing spermathecae of female bumblebees is made:

[0077] Step 1: Cutting and forming: Use 304 stainless steel round bars with a diameter of 0.3mm as raw materials, cut the round bars into 5cm length, and retain 0.5mm processing allowance, and grind the surface of the cut knife blank, and perform surface cleaning treatments such as degreasing and decontamination.

[0078] Step 2, groove processing: use the control system of the CNC machine tool to accurately set the processing path, depth, speed and other parameters, and process the tip of the tool to form a groove with a diameter of 0.23mm and a depth of 2mm as the capsule removal groove 111, which is parallel to the axis of the tool body 1, and the center of the capsule removal groove 111 coincides with the center of the tool body 1; the diameter of the hemispherical auxiliary groove 121 at the tail of the tool is 0.26mm, and the drilling depth is 0.2mm.

[0079] Step 3, tool tip bevel processing: high-precision wire cutting technology is used to accurately process the bevel tool tip on the tool tip portion 11 of the tool blank, and the feed speed and cutting depth of the wire cutting are controlled to process the bevel tool tip with an inclination angle of 30° and a length of 2 mm.

[0080] Step 4: Processing of the hemisphere at the end of the cutter: The control system of the CNC machine tool accurately sets the processing path, depth, speed and other parameters to process a hemisphere that is consistent with the diameter of the round rod.

[0081] Step 5, overall surface processing of the blade body 1: Polishing preparation: prepare polishing wheels and polishing pastes of different grits, as well as necessary polishing equipment and fixtures; Rough polishing: use a coarser-grained polishing wheel to roughly polish the entire scalpel to remove larger surface roughness; Medium polishing: use a medium-grained polishing wheel and polishing paste to further refine the roughness of the scalpel surface; Fine polishing: use a fine-grained polishing wheel and polishing paste for fine polishing until the surface of the scalpel reaches the required smoothness.

[0082] Step 6: Cleaning and inspection: After polishing, clean thoroughly and inspect whether the various indicators of the scalpel meet the design requirements.

[0083] Embodiment 3:

[0084] According to the spermathecae data of the queen of the red-light bumblebee, a scalpel for removing the spermathecae of female bumblebees is made:

[0085] Step 1, cutting and forming: select 304 stainless steel round bars with a diameter of 0.31mm as raw materials, cut the round bars into a length of 5cm, and retain a processing allowance of 0.5mm, and grind the surface of the cut knife blank, and perform surface cleaning treatments such as degreasing and decontamination;

[0086] Step 2, groove processing: use the control system of the CNC machine tool to accurately set the processing path, depth, speed and other parameters, and the tool tip processes a capsule removal groove 111 with a diameter of 0.24mm and a depth of 2mm, which is parallel to the axis of the tool body 1, and the center of the capsule removal groove 111 coincides with the center of the tool body 1; the hemispherical auxiliary groove 121 at the tool tail has a diameter of 0.26mm and a drilling depth of 0.2mm.

[0087] Step 3, tool tip bevel processing: high-precision wire cutting technology is used to accurately process the bevel tool tip on the tool tip portion 11 of the tool blank, and the feed speed and cutting depth of the wire cutting are controlled to process the bevel tool tip with an inclination angle of 30° and a length of 2 mm.

[0088] Step 4: Processing of the hemisphere at the end of the cutter: The control system of the CNC machine tool accurately sets the processing path, depth, speed and other parameters to process a hemisphere that is consistent with the diameter of the round rod.

[0089] Step 5, overall surface processing of the blade body 1: Polishing preparation: prepare polishing wheels and polishing pastes of different grits, as well as necessary polishing equipment and fixtures; Rough polishing: use a coarser-grained polishing wheel to roughly polish the entire scalpel to remove larger surface roughness; Medium polishing: use a medium-grained polishing wheel and polishing paste to further refine the roughness of the scalpel surface; Fine polishing: use a fine-grained polishing wheel and polishing paste for fine polishing until the surface of the scalpel reaches the required smoothness.

[0090] Step 6: Cleaning and inspection: After polishing, clean thoroughly and inspect whether the various indicators of the scalpel meet the design requirements.

[0091] Embodiment 4:

[0092] According to the spermathecae data of the queen of the green bumblebee, a scalpel for removing the spermathecae of female bumblebees is made:

[0093] Step 1, cutting and forming: select 304 stainless steel round bars with a diameter of 0.5mm as raw materials, cut the round bars into 5cm length, and keep 0.5mm processing allowance, and grind the surface of the cut knife blank, and perform surface cleaning treatment such as degreasing and decontamination;

[0094] Step 2, groove processing: use the control system of the CNC machine tool to accurately set the processing path, depth, speed and other parameters, and the tool tip processes a capsule removal groove 111 with a diameter of 0.4mm and a depth of 2mm, which is parallel to the axis of the tool body 1, and the center of the capsule removal groove 111 coincides with the center of the tool body 1; the hemispherical auxiliary groove 121 at the tool tail has a diameter of 0.26mm and a drilling depth of 0.2mm.

[0095] Step 3, tool tip bevel processing: high-precision wire cutting technology is used to accurately process the bevel tool tip on the tool tip portion 11 of the tool blank, and the feed speed and cutting depth of the wire cutting are controlled to process the bevel tool tip with an inclination angle of 30° and a length of 2 mm.

[0096] Step 4: Processing of the hemisphere at the end of the cutter: The control system of the CNC machine tool accurately sets the processing path, depth, speed and other parameters to process a hemisphere that is consistent with the diameter of the round rod.

[0097] Step 5, overall surface processing of the blade body 1: Polishing preparation: prepare polishing wheels and polishing pastes of different grits, as well as necessary polishing equipment and fixtures; Rough polishing: use a coarser-grained polishing wheel to roughly polish the entire scalpel to remove larger surface roughness; Medium polishing: use a medium-grained polishing wheel and polishing paste to further refine the roughness of the scalpel surface; Fine polishing: use a fine-grained polishing wheel and polishing paste for fine polishing until the surface of the scalpel reaches the required smoothness.

[0098] Step 6: Cleaning and inspection: After polishing, clean thoroughly and inspect whether the various indicators of the scalpel meet the design requirements.

[0099] Through the description of the above embodiments, those skilled in the art can clearly understand that each embodiment has at least the following beneficial effects:

[0100] 1. Improve surgical accuracy: The design of the scalpel fully considers the deep location of the female bumblebee spermatheca and the surrounding complex structure; the bevel of the blade tip and the spermatheca removal groove 111 are designed according to the characteristics of the spermatheca, so that the scalpel can more accurately locate and remove the spermatheca, greatly improving the accuracy of the operation and reducing the damage caused by misoperation.

[0101] 2. Reduce the damage to bumblebees: Through sophisticated craftsmanship, the tip of the scalpel and the cyst removal groove 111 are precisely sized and smooth, reducing the friction and damage to the bumblebee's internal tissues during surgery; at the same time, the appropriate tilt angle and the design of the cyst removal groove 111 help reduce resistance during surgery, further reducing trauma to the bumblebees.

[0102] 3. Improve the success rate of surgery: The design and ease of operation of the scalpel make the surgical process smoother and improve the success rate of the surgery; in addition, the high stability and precision of the scalpel also ensure the reliability of the surgical results and provide more accurate experimental data for subsequent entomological research.

[0103] 4. Ensure the survival of bumblebees: Minimizing damage to bumblebees during surgery means a higher survival rate for bumblebees after surgery; this is crucial for entomological research that requires long-term observation or follow-up experiments.

[0104] 5. Process standardization and repeatability: The manufacturing process proposed in the present invention is detailed and standardized, including the steps of cutting and forming, groove processing, blade tip bevel processing and overall surface processing of the blade body 1, which ensures the consistency of quality and performance of each scalpel; this repeatability not only improves production efficiency, but also ensures the reliability of the comparability of experimental results.

[0105] 6. Reasonable material selection: Using 304 stainless steel as the material for the scalpel not only ensures the strength and durability of the scalpel, but also has good corrosion resistance and high temperature resistance, which is conducive to disinfection.

[0106] 7. Promote bumblebee research: The scalpel and its manufacturing process of the present invention provide an effective tool for studying bumblebee reproduction, which can more deeply explore the structure and function of bumblebee reproductive tissues, and also provide valuable reference for studying other insects.

[0107] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A surgical knife for removing the spermatheca of a bumblebee, characterized in that: include: The blade body has a cylindrical structure; The blade tip of the knife body is provided with an inclined surface to form an inclined cutting tip structure, and the knife body located at the inclined surface section is provided with a sac-taking groove, the sac-taking groove extends along the axis direction of the knife body, and the sac-taking groove is used to temporarily store the sperm sac; The tail of the knife body is provided with an auxiliary groove for cooperating with the bevel cutting tip to take out the spermatheca.

2. The surgical knife for removing the bumblebee spermatheca according to claim 1, characterized in that: The end of the knife tail has a hemispherical structure.

3. The surgical knife for removing the bumblebee spermatheca according to claim 1, characterized in that: The knife body is also provided with a cross-cutting plane, and the cross-cutting plane is located at one end of the knife body where the auxiliary groove is located.

4. The surgical knife for removing the bumblebee spermatheca according to claim 1, characterized in that: The diameter of the capsule removal groove is 0.22-0.5 mm, and the depth of the capsule removal groove is 2 mm.

5. The surgical knife for removing the bumblebee spermatheca according to claim 1, characterized in that: The center line of the auxiliary groove is perpendicular to the axial direction of the knife body, and the depth of the auxiliary groove is smaller than the diameter of the knife body.

6. The surgical knife for removing the bumblebee spermatheca according to claim 5, characterized in that: The diameter of the auxiliary groove is 0.22-0.5 mm, and the depth of the auxiliary groove is 0.2 mm.

7. The surgical knife for removing the spermatheca of bumblebees according to claim 1, characterized in that: The inclination angle of the inclined surface is 30-35°.

8. The surgical knife for removing the bumblebee spermatheca according to claim 1, characterized in that: The knife body is a cylindrical structure.

9. A process for preparing a surgical knife for removing bumblebee spermatheca as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: S10, selecting a columnar material of 304 stainless steel as the raw material of the knife body and cutting it into shape, retaining a processing allowance to form a knife blank, and performing preliminary grinding and decontamination on the surface of the knife blank to form a knife body; S20, machining a tip hole at the tip of the knife body using a machining device, and performing a bevel cutting at the tip position using a wire cutting machine to form a beveled tip structure and a capsule removal groove at the tip position; S30, using a processing device to process a hemispherical structure at the tail of the cutter body, and to process an auxiliary groove at the tail of the cutter body; S40, finely polishing the entire blade body, and inspecting it after polishing to obtain a qualified surgical knife.

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