Immunomagnetic bead preparation system and method

Abstract

The invention discloses an immunomagnetic bead preparation system and method. The immunomagnetic bead preparation system comprises a closed pipeline; the magnetic bead sample containing piece is communicated with the closed pipeline; the oxidation liquid containing piece is communicated with the closed pipeline; the coating liquid containing part is communicated with the closed pipeline; the non-specific interface confining liquid containing piece is communicated with the closed pipeline; the uniform mixing piece is communicated with the closed pipeline; and the pipeline control assembly is arranged on the closed pipeline. When the immunomagnetic beads are prepared, the magnetic bead sample containing part, the oxidation liquid containing part, the coating liquid containing part, the non-specific interface confining liquid containing part and the uniform mixing part are sequentially conducted through the pipeline control assembly, so that oxidation and coating of the magnetic beads are achieved, non-specific interfaces in the surfaces of the coated magnetic beads are sealed, and finally the immunomagnetic beads are obtained. The whole process of preparing the immune cells is carried out in a closed space, so that a sterile environment is ensured, and batch quality and mass production application are ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of immunomagnetic bead preparation, and in particular to an immunomagnetic bead preparation system and method. Background Art

[0002] Immunomagnetic beads are a new immunological technology developed in recent years. It combines the unique advantages of solidifying reagents with the high specificity of immunological reactions. Based on immunology, it penetrates into various fields such as pathology, physiology, pharmacology, microbiology, biochemistry and molecular genetics. It has been more and more widely used in immunoassays, cell separation, biomacromolecule purification and molecular biology. The core-shell synthesis method is used to synthesize composite materials covered with polymer surfaces containing superparamagnetic substances, substances with good stability and capable of later labeling. The functional groups on the surfaces of these substances, such as amino, carboxyl, thiol, etc., are used for covalent or non-covalent coupling of antibodies, which can be used to bind to the corresponding antigens or antibodies. In this way, they can be directed to move under the attraction of an external magnetic field, thereby achieving the purpose of separation, detection and purification of genes, proteins, cells, microorganisms, etc.

[0003] Currently, the preparation of immunomagnetic beads is usually done manually, which makes it difficult to ensure a sterile environment, batch quality, and mass production applications. Summary of the invention

[0004] In view of this, the first object of the present invention is to provide an immunomagnetic bead preparation system, aiming to ensure a sterile environment, batch quality and mass production application.

[0005] The second object of the present invention is to provide a method for preparing immunomagnetic beads.

[0006] In order to achieve the above first object, the present invention provides the following scheme:

[0007] An immunomagnetic bead preparation system, comprising:

[0008] Closed pipeline;

[0009] A magnetic bead sample holding component connected to the closed pipeline;

[0010] an oxidizing liquid containing member communicated with the sealed pipeline;

[0011] a coating liquid containing member communicated with the sealed pipeline;

[0012] A non-specific interface sealing liquid containing component communicated with the closed pipeline;

[0013] a mixing element connected to the closed pipeline;

[0014] A pipeline control component is arranged on the closed pipeline, and the pipeline control component is used to control the magnetic bead sample holding part, the oxidizing liquid holding part, the coating liquid holding part and the non-specific interface sealing liquid holding part to be connected with the mixing part respectively in sequence, so as to input the magnetic bead sample into the mixing part, input the oxidizing liquid into the mixing part to oxidize the magnetic beads, input the coating liquid into the mixing part to coat the oxidized magnetic beads, and input the non-specific interface sealing liquid into the mixing part to seal the non-specific interface on the surface of the coated magnetic beads to obtain immunomagnetic beads.

[0015] In a specific embodiment, the immunomagnetic bead preparation system further comprises a power drive;

[0016] The power driving member is installed on the closed pipeline and is used to drive the liquid in the closed pipeline to move along a first preset path of the closed pipeline.

[0017] In another specific embodiment, the immunomagnetic bead preparation system further comprises a waste liquid collection component and a magnetic field loading and unloading mechanism;

[0018] The closed pipeline is provided with a separation column, and the magnetic field loading and unloading mechanism is used to load or unload the magnetic field to the separation column;

[0019] The waste liquid collecting component is in communication with the closed pipeline, and the pipeline control component is also used to control the mixing component and the waste liquid collecting component to be in communication with the separation column respectively;

[0020] When the waste liquid discharged from the mixing element needs to be discharged, the mixing element and the waste liquid collecting element are respectively connected to the separation column, and the magnetic field loading and unloading mechanism loads the magnetic field to the separation column.

[0021] In another specific embodiment, the immunomagnetic bead preparation system further comprises a target collection piece;

[0022] The target collecting piece is in communication with the closed pipeline, and the pipeline control assembly is also used to control the separation column to be in communication with the target collecting piece.

[0023] In another specific embodiment, the immunomagnetic bead preparation system further comprises a cleaning solution container;

[0024] The cleaning liquid holding component is connected to the closed pipeline, and the pipeline control component is also used to control the cleaning liquid in the cleaning liquid holding component to flow along a second preset path of the closed pipeline to clean the closed pipeline and / or various components connected to the closed pipeline.

[0025] In another specific embodiment, the cleaning solution holding member includes a buffer solution holding member and a saline solution holding member respectively connected to the closed pipeline;

[0026] The salt solution in the salt solution container can clean the oxidized magnetic beads.

[0027] In another specific embodiment, the immunomagnetic bead preparation system further comprises an airtightness detection component;

[0028] The air tightness detection component is installed on the closed pipeline and is used to detect the air tightness of the closed pipeline.

[0029] In another specific embodiment, the pipeline control assembly includes a plurality of switch valves clamped outside the closed pipeline;

[0030] and / or,

[0031] The power driving member is a peristaltic pump clamped outside the closed pipeline;

[0032] and / or,

[0033] The mixing unit comprises a centrifuge and a centrifuge bag arranged in the centrifuge, and the centrifuge bag is rotatable and connected to the closed pipeline.

[0034] The various embodiments according to the present invention can be arbitrarily combined as needed, and the embodiments obtained after these combinations are also within the scope of the present invention and are part of the specific implementation methods of the present invention.

[0035] In order to achieve the above second object, the present invention provides the following solution:

[0036] A method for preparing immunomagnetic beads, comprising:

[0037] Providing an immunomagnetic bead preparation system as described in any one of the above;

[0038] Connecting the magnetic bead sample holding part and the mixing part in the immunomagnetic bead preparation system, and inputting the magnetic bead sample into the mixing part;

[0039] connecting the oxidizing liquid containing part and the mixing part in the immunomagnetic bead preparation system, and inputting the oxidizing liquid into the mixing part to oxidize the magnetic beads;

[0040] Connecting the coating solution containing component and the mixing component in the immunomagnetic bead preparation system, and inputting the coating solution into the mixing component to coat the oxidized magnetic beads;

[0041] The nonspecific interface blocking liquid containing part and the mixing part in the immunomagnetic bead preparation system are connected, and the nonspecific interface blocking liquid is input into the mixing part to block the nonspecific interface on the surface of the coated magnetic beads to obtain the immunomagnetic beads.

[0042] In another specific embodiment, after inputting each component into the mixing element, the step includes: washing the magnetic beads.

[0043] In another specific embodiment, washing the magnetic beads comprises:

[0044] Starting the magnetic field loading and unloading mechanism in the immunomagnetic bead preparation system to load the magnetic field to the sorting column, connecting the mixing element and the sorting column in the immunomagnetic bead preparation system, connecting the sorting column and the waste liquid collecting element in the immunomagnetic bead preparation system, adsorbing the magnetic beads into the sorting column, and allowing the waste liquid to enter the waste liquid collecting element;

[0045] Connecting the cleaning liquid holding part and the mixing part in the immunomagnetic bead preparation system, flushing the mixing part and the separation column with the cleaning liquid in the cleaning liquid holding part, and discharging the waste liquid into the waste liquid collecting part;

[0046] The separation column is connected to the mixing element or the target collection element in the immunomagnetic bead preparation system, the magnetic field loaded to the separation column by the magnetic field loading and unloading mechanism is unloaded, and the magnetic beads in the separation column are transported to the mixing element or the target collection element.

[0047] In another specific embodiment, when cleaning the oxidized magnetic beads, the cleaning liquid holding part includes a buffer solution holding part and a salt solution holding part, and connecting the cleaning liquid holding part and the mixing part includes: connecting the salt solution holding part and the mixing part, and flushing the mixing part and the sorting column with the salt solution; connecting the buffer solution holding part and the mixing part, and flushing the mixing part and the sorting column with the buffer solution.

[0048] In another specific embodiment, the magnetic bead sample holding part and the mixing part in the immunomagnetic bead preparation system are turned on, and the magnetic bead sample is input into the mixing part, which specifically includes:

[0049] The magnetic field loading and unloading mechanism is started to load the magnetic field to the sorting column, the magnetic bead sample holding part and the sorting column are connected, and the sorting column and the waste liquid collecting part are connected, so that the magnetic beads output by the magnetic bead sample holding part are adsorbed in the sorting column, and the waste liquid is discharged into the waste liquid collecting part;

[0050] Disconnecting the magnetic bead sample holding part from the separation column, and connecting the buffer solution holding part to the separation column, so that the buffer solution in the buffer solution holding part flows through the separation column to wash the magnetic beads, and is discharged into the waste liquid collection part;

[0051] The separation column and the waste liquid collection part are disconnected, and the separation column and the mixing part are connected, and the magnetic field loading and unloading mechanism is closed to close the magnetic field of the separation column, so that the buffer solution flows through the separation column and transports the magnetic beads into the mixing part.

[0052] In another specific embodiment, before inputting the magnetic bead sample into the magnetic bead sample holding member, the method further comprises:

[0053] Detecting the air tightness of the immunomagnetic bead preparation system by using the air tightness detection component of the immunomagnetic bead preparation system;

[0054] The immunomagnetic bead preparation system is rinsed.

[0055] The various embodiments according to the present invention can be arbitrarily combined as needed, and the embodiments obtained after these combinations are also within the scope of the present invention and are part of the specific implementation methods of the present invention.

[0056] The immunomagnetic bead preparation system provided by the present invention, when preparing immunomagnetic beads, the magnetic bead sample holding part and the mixing part are connected through the pipeline control component, and the sample of the magnetic beads is input into the mixing part; then, the oxidation liquid holding part and the mixing part are connected through the pipeline control component, and the oxidation liquid is input into the mixing part to oxidize the magnetic beads; then, the coating liquid holding part and the mixing part are connected through the pipeline control component, and the coating liquid is input into the mixing part to coat the oxidized magnetic beads; then, the non-specific interface sealing liquid holding part and the mixing part are connected through the pipeline control component, and the non-specific interface sealing liquid is input into the mixing part to seal the non-specific interface on the surface of the coated magnetic beads, and finally the immunomagnetic beads are obtained. Since the entire process of preparing immune cells is carried out in a closed space, a sterile environment is guaranteed, and batch quality and mass production applications are guaranteed. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without expending any novel work.

[0058] Figure 1 A schematic diagram of the main structure of an immunomagnetic bead preparation system provided by an embodiment of the present invention;

[0059] Figure 2 A schematic diagram of the main structure of the immunomagnetic bead preparation system provided by one embodiment of the present invention when the pipeline control component is not installed;

[0060] Figure 3 A schematic diagram of the main structure of an immunomagnetic bead preparation system provided by another embodiment of the present invention;

[0061] Figure 4 A schematic diagram of the main structure of an immunomagnetic bead preparation system provided by another embodiment of the present invention when a pipeline control component is not installed;

[0062] Figure 5 The present invention is a schematic diagram of the top view of the centrifuge bag.

[0063] in, Figure 1-Figure 5 middle:

[0064] Immunomagnetic bead preparation system 100, closed pipeline 101, sorting column 101-1, mixing element 102, centrifuge bag 102-1, pipeline control component 103, first switch valve 103-1, second switch valve 103-2, third switch valve 103-3, fourth switch valve 103-4, fifth switch valve 103-5, sixth switch valve 103-6, seventh switch valve 103-7, eighth switch valve 103-8, ninth switch valve 103-9, tenth switch valve 103-10, eleventh switch valve 103-11, twelfth switch valve 103-12, thirteenth switch valve 103-13, fourteenth switch valve 103-14, fifteenth switch valve 103-15, sixteenth switch valve 103-16 03-16, the seventeenth switch valve 103-17, the eighteenth switch valve 103-18, the nineteenth switch valve 103-19, the twentieth switch valve 103-20, the twenty-first switch valve 103-21, the twenty-second switch valve 103-22, the twenty-third switch valve 103-23, the power driving part 104, the waste liquid collecting part 105, the magnetic field loading and unloading mechanism 106, the destination collecting part 107, the cleaning liquid holding part 108, the first buffer solution holding part 108-1, the second buffer solution holding part 108-2, the air tightness detection component 109, the first sensor 109-1, the second sensor 109-2, the pre-sorting column 110, the backwash waste liquid holding part 111, and the rotary joint 112. DETAILED DESCRIPTION

[0065] The following will be combined with the attached embodiment of the present invention Figure 1-Figure 5 , the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0066] In the description of the present invention, it should be understood that the terms "upper", "lower", "top surface", "bottom surface" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the positions or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limitations of the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0067] Combination Figure 1-Figure 5 As shown, the first aspect of the present invention provides an immunomagnetic bead preparation system 100, which can provide a sterile environment for the preparation of immunomagnetic beads, thereby ensuring the batch quality and mass production application of the immunomagnetic beads.

[0068] The immunomagnetic bead preparation system 100 includes a closed pipeline 101 , a magnetic bead sample container, an oxidizing liquid container, a coating liquid container, a non-specific interface sealing liquid container, a mixing component 102 and a pipeline control component 103 .

[0069] Specifically, the sealed pipeline 101 can be a disposable pipeline consumable, and of course, it can also be other types of pipelines.

[0070] The closed pipeline 101 has a first interface, a second interface, a third interface, a fourth interface and a fifth interface, wherein the magnetic bead sample holder is connected to the first interface, the oxidizing liquid holder is connected to the second interface, the coating liquid holder is connected to the third interface, the non-specific interface sealing liquid holder is connected to the fourth interface, and the mixing unit 102 is connected to the fifth interface. It should be noted that the communication mentioned herein is all communication under closed conditions.

[0071] The pipeline control component 103 is arranged on the closed pipeline 101. Specifically, the pipeline control component 103 can be a switch valve group composed of multiple switch valves. More specifically, the switch valves are arranged at corresponding positions of the closed pipeline 101. In order to facilitate the control of the opening and closing of each switch valve, the switch valve can be set as a solenoid valve.

[0072] The pipeline control component 103 is used to control the magnetic bead sample holding part, the oxidizing liquid holding part, the coating liquid holding part and the non-specific interface sealing liquid holding part to be connected with the mixing part 102 in sequence, so as to input the magnetic bead sample into the mixing part 102, input the oxidizing liquid into the mixing part 102 to oxidize the magnetic beads, input the coating liquid into the mixing part 102 to coat the oxidized magnetic beads, and input the non-specific interface sealing liquid into the mixing part 102 to seal the non-specific interface on the surface of the coated magnetic beads to obtain immunomagnetic beads.

[0073] Since the entire process of preparing immune cells is carried out in a closed space, a sterile environment is guaranteed, ensuring batch quality and mass production applications.

[0074] In some embodiments, it is specifically disclosed that the mixing element 102 includes a centrifuge and a centrifuge bag 102-1. The centrifuge bag 102-1 is arranged in the centrifuge and can perform centrifugal motion with the centrifuge. A rotary joint 112 is installed on the centrifuge. The centrifuge bag 102-1 is connected to the fifth interface through the rotary joint 112 to achieve communication between the rotating part (centrifuge bag 102-1) and the stationary part (closed pipeline 101).

[0075] In some embodiments, the immunomagnetic bead preparation system 100 further includes a power driver 104 , which is installed on the closed pipeline 101 and is used to drive the liquid in the closed pipeline 101 to move along a first preset path of the closed pipeline 101 .

[0076] Specifically, the power driving component 104 is a peristaltic pump or the like. To facilitate the disassembly and assembly of the power driving component 104 , the power driving component 104 can be clamped on the closed pipeline 101 .

[0077] It can be understood that the power driving component 104 being a peristaltic pump is only a specific embodiment of the present invention. In practical applications, the power driving component 104 can also be set as other driving components that can provide power to the liquid in the closed pipeline 101.

[0078] Specifically, the first preset path is any path in any closed pipeline 101 passing through the power drive component 104 .

[0079] In some embodiments, the immunomagnetic bead preparation system 100 further includes a waste liquid collection component 105 and a magnetic field loading and unloading mechanism 106. The closed pipeline 101 is also provided with a sixth interface, and the waste liquid collection component 105 is connected to the sixth interface for collecting waste liquid.

[0080] A separation column 101 - 1 is provided on the closed pipeline 101 , and the pipeline control component 103 is also used to control the mixing element 102 and the waste liquid collecting element 105 to be connected to the separation column 101 - 1 respectively.

[0081] The magnetic field loading and unloading mechanism 106 is used to load or unload the magnetic field to the sorting column 101-1. Specifically, the magnetic field loading and unloading mechanism 106 includes a permanent magnet and a power part. The permanent magnet is provided with a receiving slot for accommodating the sorting column 101-1. The driving end of the power part is connected to the permanent magnet, and is used to drive the permanent magnet close to or away from the sorting column 101-1 to apply a magnetic field to the sorting column 101-1 or disconnect the magnetic field. The power part can be a gear rack structure, an electric push rod structure, a hydraulic cylinder or a cylinder structure. It should be noted that the structure of the magnetic field loading and unloading mechanism 106 disclosed above is only a specific embodiment of the present application. In practical applications, the magnetic field loading and unloading mechanism 106 can also be set to other structures. For example, the magnetic field loading and unloading mechanism 106 includes an electromagnet and a power supply part. The electromagnet is provided with a receiving slot for accommodating the sorting column 101-1. The power supply part is electrically connected to the electromagnet, and the power supply part energizes or deenergizes the electromagnet to apply a magnetic field to the sorting column 101-1 or disconnect the magnetic field.

[0082] When it is necessary to discharge the waste liquid discharged from the mixing element 102, the mixing element 102 and the waste liquid collecting element 105 are respectively connected to the sorting column 101-1, and the magnetic field loading and unloading mechanism 106 loads the magnetic field to the sorting column 101-1. At this time, the magnetic beads coming out of the mixing element 102 can be adsorbed in the sorting column 101-1, and the waste liquid flows through the sorting column 101-1 and enters the waste liquid collecting element 105.

[0083] Furthermore, the immunomagnetic bead preparation system 100 disclosed in the present invention also includes a pre-sorting column 110. When each component is input into the sorting column 101-1, it first flows through the pre-sorting column 110 and then enters the sorting column 101-1.

[0084] In order to facilitate the collection of immunomagnetic beads, the immunomagnetic bead preparation system 100 disclosed in the present invention also includes a destination collecting piece 107, and the closed pipeline 101 is also provided with a seventh interface, the destination collecting piece 107 is connected to the seventh interface, and the pipeline control component 103 is also used to control the connection between the sorting column 101-1 and the destination collecting piece 107.

[0085] In order to facilitate the transfer of the magnetic beads in the sorting column 101-1 to the mixing piece 102 or the target collection piece 107, the present invention discloses that the immunomagnetic bead preparation system 100 also includes a cleaning liquid holding piece 108, wherein the closed pipeline 101 is also provided with an eighth interface, and the cleaning liquid holding piece 108 is connected to the eighth interface.

[0086] The pipeline control component 103 is also used to control the cleaning liquid in the cleaning liquid holding part 108 to flow along the second preset path of the closed pipeline 101 to clean the closed pipeline 101 and / or various components connected to the closed pipeline 101.

[0087] Specifically, the second preset path includes: any path connecting the cleaning liquid containing part 108 and the waste liquid collecting part 105; any path connecting the components of the closed pipeline 101 and the waste liquid collecting part 105, etc.

[0088] Of course, the cleaning liquid in the cleaning liquid holding component 108 can enter the closed pipeline 101 for cleaning under the action of gravity, and finally enter the waste liquid collecting component 105. The cleaning liquid in the cleaning liquid holding component 108 can also be driven by the power driving component 104 to enter the closed pipeline 101 for cleaning, and finally enter the waste liquid collecting component 105. At this time, the path is a path connecting the cleaning liquid holding component 108 and the waste liquid collecting component 105, and passing through the power driving component 104.

[0089] When it is necessary to collect the magnetic beads in the sorting column 101-1 into the mixing element 102, it is only necessary to connect the sorting column 101-1 and the mixing element 102, and the cleaning liquid holding element 108 and the sorting column 101-1, and disconnect the magnetic field of the sorting column 101-1. The power driving element 104 is activated to transport the cleaning liquid in the cleaning liquid holding element 108 into the sorting column 101-1, driving the magnetic beads in the sorting column 101-1 into the mixing element 102 or the target collection element 107 together with the cleaning liquid.

[0090] It should be noted that the cleaning liquid container 108 can also be used for rinsing the sealed pipeline 101 before use.

[0091] Further, the present invention discloses that the cleaning liquid holding part 108 includes a buffer solution holding part and a salt solution holding part. Specifically, the buffer solution holding part includes a first buffer solution holding part 108-1 and a second buffer solution holding part 108-2. The first buffer solution holding part 108-1 is connected to the closed pipeline 101, and the connection point is located upstream of the magnetic bead sample holding part, the oxidation liquid holding part, the coating liquid holding part, and the non-specific interface sealing liquid holding part. It should be noted that the upstream here refers to the upstream along the flow direction of loading each component into the mixing part 102.

[0092] The second buffer storage part 108 - 2 is connected to the sealed pipeline 101 , and the connecting point is located between the pre-sorting column 110 and the sorting column 101 - 1 , and can store the buffer.

[0093] Specifically, the first buffer container 108-1 can transport a certain amount of buffer to the second buffer container 108-2 after rinsing the closed pipeline 101. Of course, the second buffer container 108-2 can also be directly loaded with buffer.

[0094] In order to collect the waste liquid generated after the backwashing separation column 101 - 1 , a closed pipeline 101 may be provided to be connected to a backwashing waste liquid receiving member 111 .

[0095] It should be noted that when a backwash waste liquid holding part 111 is provided, and the second preset path also includes components such as a backwash sorting column 101-1, the backwash waste liquid holding part 111 and the cleaning liquid holding part 108 are connected, and the path flowing through components such as the sorting column 101-1, a power driving part 104 can be provided to drive the cleaning liquid in the cleaning liquid holding part 108 to flow through components such as the sorting column 101-1 and into the backwash waste liquid holding part 111.

[0096] It should also be noted that the reverse flushing here refers to the direction opposite to the direction of the components through which the cleaning liquid flows when the cleaning liquid holding part 108 and the waste liquid collecting part 105 are connected. Figure 1 Taking the direction in as an example, when the sorting column 101-1 is flushed forward, it means that the cleaning liquid flows through the sorting column 101-1 from top to bottom, and when the sorting column 101-1 is flushed reversely, it means that the cleaning liquid flows through the sorting column 101-1 from bottom to top.

[0097] In some embodiments, the immunomagnetic bead preparation system 100 further includes an airtightness detection component 109 , which is installed on the closed pipeline 101 and is used to detect the airtightness of the closed pipeline 101 .

[0098] Specifically, the airtightness detection component 109 may be a sensor group, which includes a plurality of sensors distributed at various positions of the closed pipeline 101. Figure 1 As shown, the airtightness detection component 109 includes a first sensor 109-1 and a second sensor 109-2, wherein the first sensor 109-1 is located at one end of the power driving member 104, and the second sensor 109-2 is located at the other end of the power driving member 104. The power driving member 104 cooperates with the pipeline control component 103 to detect whether the seal is good according to the pressure values ​​of the first sensor 109-1 and the second sensor 109-2.

[0099] In order to facilitate the detection of whether the mixing part 102 is completely output, a bubble sensor can be installed at a position close to the fifth interface of the closed pipeline 101. Similarly, bubble sensors can also be installed at positions close to the first interface, the second outlet, the third outlet and the fourth outlet of the closed pipeline 101, respectively, to respectively detect whether the magnetic bead sample output by the magnetic bead sample holding part is completely output, whether the oxidizing liquid in the oxidizing liquid holding part is completely output, whether the coating liquid in the coating liquid holding part is completely output, and whether the non-specific interface sealing liquid in the non-specific interface sealing liquid holding part is completely output. Of course, the magnetic bead sample holding part, the oxidizing liquid holding part, the coating liquid holding part and the non-specific interface sealing liquid holding part can also be arranged on the same pipeline of the closed pipeline 101, and a bubble sensor can be installed at the end of the pipeline.

[0100] The second aspect of the present invention provides a method for preparing immunomagnetic beads, comprising:

[0101] Step S1: providing an immunomagnetic bead preparation system 100;

[0102] Specifically, the immunomagnetic bead preparation system 100 is the immunomagnetic bead preparation system 100 in any one of the above embodiments.

[0103] Step S2: inputting the magnetic bead sample into the mixing element 102 .

[0104] Specifically, the magnetic bead sample holding part and the mixing part 102 in the immunomagnetic bead preparation system 100 are connected to input the magnetic bead sample into the mixing part 102. The amount of the magnetic bead sample input into the mixing part 102 can be measured by a flow meter disposed at the outlet of the magnetic bead sample holding part.

[0105] More specifically, the sample of magnetic beads is magnetic beads that have been coated with a hydroxypolysaccharide.

[0106] Step S3: injecting an oxidizing solution into the mixing unit 102 to oxidize the magnetic beads.

[0107] Specifically, the oxidizing liquid containing part and the mixing part 102 in the immunomagnetic bead preparation system 100 are turned on to input the oxidizing liquid into the mixing part 102 .

[0108] It should be noted that the oxidizing solution may be a sodium salt solution, and the oxidized magnetic beads refer to the hydroxy polysaccharide coated with the oxidized magnetic beads being an aldehyde polysaccharide.

[0109] Step S4: injecting coating solution into the mixing unit 102 to coat the oxidized magnetic beads.

[0110] Specifically, the coating solution containing part and the mixing part 102 in the immunomagnetic bead preparation system 100 are connected to input the coating solution into the mixing part 102 .

[0111] More specifically, the coating solution is an antibody or the like.

[0112] Step S5: non-specific interface blocking solution is input into the mixing unit 102 to block the non-specific interfaces on the surface of the coated magnetic beads to obtain immunomagnetic beads.

[0113] Specifically, the non-specific interface blocking liquid containing part and the mixing part 102 in the immunomagnetic bead preparation system 100 are turned on to input the non-specific interface blocking liquid into the mixing part 102 .

[0114] More specifically, the non-specific interface blocking solution is a protein solution or the like.

[0115] In order to ensure that the entire system is well sealed, step S6 is further included between step S1 and step S2: detecting the airtightness of the immunomagnetic bead preparation system 100 by using the airtightness detection component 109 of the immunomagnetic bead preparation system 100 .

[0116] In order to prevent impurities in the closed pipeline 101 from affecting the purity of the immunomagnetic beads, step S7 is also included between step S6 and step S2: rinsing the immunomagnetic bead preparation system 100.

[0117] In order to avoid mutual influence between components in adjacent steps, the present invention discloses that step S2, step S3, step S4 and step S5 all include step S8: washing magnetic beads.

[0118] Specifically, step S8 includes:

[0119] Step S81: Load the magnetic field to the separation column 101 - 1 to adsorb the magnetic beads into the separation column 101 - 1 , and the waste liquid enters the waste liquid collection part 105 .

[0120] Specifically, the magnetic field loading and unloading mechanism 106 in the immunomagnetic bead preparation system 100 is started to load the magnetic field to the sorting column 101-1, the mixing element 102 and the sorting column 101-1 in the immunomagnetic bead preparation system 100 are turned on, the sorting column 101-1 and the waste liquid collecting element 105 in the immunomagnetic bead preparation system 100 are turned on, the power driving element 104 is started, the magnetic beads are adsorbed into the sorting column 101-1, and the waste liquid enters the waste liquid collecting element 105.

[0121] Step S82: The cleaning liquid in the cleaning liquid holding part 108 rinses the mixing part 102 and the separation column 101 - 1, and discharges the waste liquid into the waste liquid collecting part 105.

[0122] The cleaning solution holding part 108 and the mixing part 102 in the immunomagnetic bead preparation system 100 are turned on to flush the mixing part 102 and the separation column 101 - 1 with the cleaning solution in the cleaning solution holding part 108 .

[0123] Specifically, the first buffer solution holding part 108 - 1 of the cleaning solution holding part 108 is electrically connected to the mixing part 102 .

[0124] Step S83: Unload the magnetic field loading and unloading mechanism 106 loaded into the magnetic field of the sorting column 101-1, and transport the magnetic beads in the sorting column 101-1 to the mixing part 102 or the target collection part 107.

[0125] The separation column 101 - 1 is connected to the mixing element 102 or the target collecting element 107 in the immunomagnetic bead preparation system 100 , so that the magnetic beads in the separation column 101 - 1 are transported to the mixing element 102 or the target collecting element 107 .

[0126] It should be noted that when the magnetic beads in the sorting column 101-1 are transported to the mixing element 102, the second buffer solution holding element 108-2 is connected to the sorting column 101-1, that is, the buffer solution is transported to the sorting column 101-1 through the second buffer solution holding element 108-2, so as to transport the magnetic beads in the sorting column 101-1 to the mixing element 102; when the magnetic beads in the sorting column 101-1 are transported to the destination collecting element 107, the first buffer solution holding element 108-1 is connected to the sorting column 101-1, that is, the buffer solution is transported to the sorting column 101-1 through the first buffer solution holding element 108-1, so as to transport the magnetic beads in the sorting column 101-1 to the destination collecting element 107.

[0127] In some embodiments, the present invention discloses that step S2 specifically includes:

[0128] Step S11: Start the magnetic field loading and unloading mechanism 106 to load the magnetic field to the sorting column 101-1, connect the magnetic bead sample holding part and the sorting column 101-1, and connect the sorting column 101-1 and the waste liquid collecting part 105, so that the magnetic beads output by the magnetic bead sample holding part are adsorbed in the sorting column 101-1, and the waste liquid is discharged into the waste liquid collecting part 105.

[0129] Step S12: disconnect the magnetic bead sample holder and the sorting column 101 - 1 , and connect the buffer solution holder and the sorting column 101 - 1 , so that the buffer solution in the buffer solution holder flows through the sorting column 101 - 1 to wash the magnetic beads and is discharged into the waste liquid collection part 105 .

[0130] Step S13: disconnect the sorting column 101-1 and the waste liquid collection component 105, and connect the sorting column 101-1 and the mixing component 102, close the magnetic field loading and unloading mechanism 106 to close the magnetic field of the sorting column 101-1, so that the buffer solution flows through the sorting column 101-1 and transports the magnetic beads to the mixing component 102.

[0131] That is to say, the magnetic bead sample input into the mixing element 102 is already a cleaned sample, and impurities in the original magnetic bead sample have been removed, thereby further improving the efficiency of preparing immunomagnetic beads.

[0132] In some embodiments, when washing the oxidized magnetic beads, the washing liquid holding part 108 includes a buffer solution holding part and a salt solution holding part, and connecting the washing liquid holding part 108 and the mixing part 102 includes: connecting the salt solution holding part and the mixing part 102, and washing the mixing part 102 and the sorting column 101-1 with the salt solution; connecting the buffer solution holding part and the mixing part 102, and washing the mixing part 102 and the sorting column 101-1 with the buffer solution. In other words, the remaining oxidation liquid and other solution impurities after the oxidation of the magnetic beads are first discharged by the salt solution, and then the oxidized magnetic beads are washed by the buffer solution in the first buffer solution holding part 108-1.

[0133] by Figure 1 Taking the example, the pipeline control component 103 includes a first switch valve 103-1, a second switch valve 103-2, a third switch valve 103-3, a fourth switch valve 103-4, a fifth switch valve 103-5, a sixth switch valve 103-6, a seventh switch valve 103-7, an eighth switch valve 103-8, a ninth switch valve 103-9, a tenth switch valve 103-10, an eleventh switch valve 103-11, a twelfth switch valve 103-12, a thirteenth switch valve 103-13, a fourteenth switch valve 103-14, a fifteenth switch valve 103-15, a sixteenth switch valve 103-16, a seventeenth switch valve 103-17, an eighteenth switch valve 103-18, and a nineteenth switch valve 103-19, and the power drive component 104 is a peristaltic pump.

[0134] The immunomagnetic bead preparation method includes:

[0135] Step A1: Air tightness test.

[0136] The air tightness of the entire immunomagnetic bead preparation system 100 is tested.

[0137] Step A2: Rinse the closed pipeline 101.

[0138] During the process of rinsing the closed pipeline 101, a certain amount of buffer solution can be transported into the second buffer solution container 108-2.

[0139] Step A3: Load the magnetic bead sample.

[0140] A magnetic field is loaded on the sorting column 101-1, and the second switch valve 103-2, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened, and the other switch valves remain closed. The peristaltic pump is started, and the peristaltic pump operates to make the magnetic bead sample (a hydroxy polysaccharide-coated nanoimmunomagnetic bead dispersion, with a magnetic bead particle size of 10 nm) flow through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the waste liquid is discharged into the waste liquid collection part 105. When the peristaltic pump is loaded to a certain amount (or after the first bubble sensor detects that the loading of the magnetic bead sample is completed), the peristaltic pump stops running and all the switch valves are closed.

[0141] It should be noted that if the sample in the magnetic bead sample holder is completely output, it is necessary to input buffer solution into the magnetic bead sample holder for multiple rinses and load it into the sorting column 101 - 1 to fully utilize the sample.

[0142] Step A4: Wash the magnetic bead sample.

[0143] Open the first switch valve 103-1, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19, and keep the other switch valves closed. Start the peristaltic pump, and operate the peristaltic pump at a certain flow rate to allow the buffer solution to flow through the pre-separation column 110 and the separation column 101-1 to rinse the other magnetic beads, and discharge the waste liquid into the waste liquid collection part 105. After the rinsing is completed, the peristaltic pump stops running and all the switch valves are closed.

[0144] Step A5: Elute the magnetic beads.

[0145] Turn off the magnetic field applied to the sorting column 101-1, open the ninth switch valve 103-9, the seventeenth switch valve 103-17, the eleventh switch valve 103-11, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and use the peristaltic pump to flush the sorting column 101-1 with a certain amount of buffer at a certain flow rate and then transport it to the centrifuge bag 102-1 of the mixing part 102. The concentration of magnetic beads in the centrifuge bag 102-1 is adjusted to 5 mg / ml. After this step is completed, the peristaltic pump stops and the switch valves are closed.

[0146] Step A6: Oxidize the magnetic beads.

[0147] Open the fourth switch valve 103-4, the tenth switch valve 103-10, the thirteenth switch valve 103-13, and the fifteenth switch valve 103-15, and the peristaltic pump rotates to add the oxidizing solution into the centrifugal bag 102-1 in a quantitative manner. The mass ratio of magnetic beads and oxidizing solution is controlled at 1:1. After completion, the peristaltic pump stops and the switch valves are closed; thereafter, the centrifuge drives the centrifugal bag 102-1 to rotate clockwise and counterclockwise according to the established procedure, and drives the centrifugal bag 102-1 to rotate and mix the magnetic beads. The mixing time is 1 hour (adjustable). After this step is completed, the peristaltic pump stops.

[0148] Step A7: Loading the oxidized magnetic beads.

[0149] A magnetic field is loaded on the sorting column 101-1, and the fifteenth switch valve 103-15, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened. The peristaltic pump allows the sample to flow through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the waste liquid is discharged into the waste liquid collection part 105. When the peristaltic pump is loaded to a certain amount or the sensor detects that the loading of the magnetic bead sample is completed, the peristaltic pump stops running and the switch valves are closed.

[0150] It should be noted that when all the magnetic bead samples in the centrifugal bag 102-1 are completely output, the centrifugal bag 102-1 needs to be rinsed multiple times and loaded into the sorting column 101-1. After completion, the peristaltic pump stops and the switch valves are closed.

[0151] Step A8: Wash the oxidized magnetic beads.

[0152] Open the sixth switch valve 103-6, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19, and operate the peristaltic pump to make the sodium salt solution flow through the pre-separation column 110 and the separation column 101-1 at a certain flow rate, and rinse the adsorbed magnetic beads therein for a certain period of time, and discharge the waste liquid into the waste liquid collection part 105. After this step is completed, the peristaltic pump stops and the switch valves are closed.

[0153] The first switch valve 103-1, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened, and the other switch valves remain closed. The peristaltic pump is started, and the peristaltic pump runs to flow the buffer solution through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the magnetic beads adsorbed therein are rinsed for a certain period of time, and the waste liquid is discharged to the waste liquid Collecting piece 105; then, turn off the magnetic field applied to the sorting column 101-1, open the ninth switch valve 103-9, the seventeenth switch valve 103-17, the eleventh switch valve 103-11, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and the peristaltic pump rotates to flush a certain amount of buffer solution with a certain flow rate to the sorting column 101-1 and then transport it to the centrifuge bag 102-1, and the concentration of magnetic beads is adjusted to 5 mg / ml. After this step is completed, the peristaltic pump stops and all the switch valves are closed.

[0154] Step A9: Coating the oxidized magnetic beads.

[0155] Open the fifth switch valve 103-5, the tenth switch valve 103-10, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and the peristaltic pump rotates to add the coating solution to the centrifugal bag 102-1 in a quantitative manner. The mass ratio of magnetic beads and antibodies is controlled at 10:1 (adjustable). After completion, the peristaltic pump stops and all the switch valves are closed; then, the centrifuge drives the centrifugal bag 102-1 to rotate clockwise and counterclockwise according to the established procedure, and drives the centrifugal bag 102-1 to rotate and mix the magnetic beads and the coating solution. The mixing time is 6 hours (adjustable). After this step is completed, the peristaltic pump stops and all the switch valves are closed.

[0156] Step A10: Load the coated magnetic beads.

[0157] A magnetic field is loaded on the sorting column 101-1, and the fifteenth switch valve 103-15, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened. The peristaltic pump allows the sample to flow through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the waste liquid is discharged into the waste liquid collection part 105. When the peristaltic pump is loaded to a certain amount or the sensor detects that the loading of the magnetic bead sample is completed, the peristaltic pump stops running and the switch valves are closed.

[0158] It should be noted that when all the magnetic bead samples in the centrifugal bag 102-1 are completely output, the centrifugal bag 102-1 needs to be rinsed multiple times and loaded into the sorting column 101-1. After completion, the peristaltic pump stops and the switch valves are closed.

[0159] Step A11: Wash the coated magnetic beads.

[0160] The first switch valve 103-1, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened, and the other switch valves remain closed. The peristaltic pump is started, and the peristaltic pump runs to flow the buffer solution through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the magnetic beads adsorbed therein are rinsed for a certain period of time, and the waste liquid is discharged to the waste liquid Collecting piece 105; then, turn off the magnetic field applied to the sorting column 101-1, open the ninth switch valve 103-9, the seventeenth switch valve 103-17, the eleventh switch valve 103-11, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and the peristaltic pump rotates to flush a certain amount of buffer solution with a certain flow rate to the sorting column 101-1 and then transport it to the centrifuge bag 102-1, and the concentration of magnetic beads is adjusted to 5 mg / ml. After this step is completed, the peristaltic pump stops and all the switch valves are closed.

[0161] Step A12: Blocking of non-specific interfaces on the surface of magnetic beads.

[0162] Open the third switch valve 103-3, the tenth switch valve 103-10, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and the peristaltic pump rotates to quantitatively add the non-specific interface blocking liquid into the centrifuge bag 102-1, and the mass ratio of magnetic beads to non-specific interface blocking liquid is controlled at 10:1 (adjustable). After completion, the peristaltic pump stops and all switch valves are closed; then the centrifuge rotates clockwise and counterclockwise according to the established program, driving the centrifuge bag 102-1 to mix the magnetic beads and the non-specific interface blocking liquid, and the mixing time is 6h (adjustable). After this step is completed, the peristaltic pump stops.

[0163] Step A13: Loading magnetic beads with blocked non-specific interfaces.

[0164] A magnetic field is loaded on the sorting column 101-1, and the fifteenth switch valve 103-15, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened. The peristaltic pump allows the sample to flow through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the waste liquid is discharged into the waste liquid collection part 105. When the peristaltic pump is loaded to a certain amount or the sensor detects that the loading of the magnetic bead sample is completed, the peristaltic pump stops running and the switch valves are closed.

[0165] It should be noted that when all the magnetic bead samples in the centrifugal bag 102-1 are completely output, the centrifugal bag 102-1 needs to be rinsed multiple times and loaded into the sorting column 101-1. After completion, the peristaltic pump stops and the switch valves are closed.

[0166] Step A14: Wash the magnetic beads that have blocked non-specific interfaces.

[0167] The first switch valve 103-1, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the thirteenth switch valve 103-13, the eighth switch valve 103-8, the seventeenth switch valve 103-17 and the nineteenth switch valve 103-19 are opened, and the other switch valves remain closed. The peristaltic pump is started, and the peristaltic pump runs to flow the buffer solution through the pre-sorting column 110 and the sorting column 101-1 at a certain flow rate, and the magnetic beads adsorbed therein are rinsed for a certain period of time, and the waste liquid is discharged to the waste liquid Collecting piece 105; then, turn off the magnetic field applied to the sorting column 101-1, open the ninth switch valve 103-9, the seventeenth switch valve 103-17, the eleventh switch valve 103-11, the thirteenth switch valve 103-13 and the fifteenth switch valve 103-15, and the peristaltic pump rotates to flush a certain amount of buffer solution with a certain flow rate to the sorting column 101-1 and then transport it to the centrifuge bag 102-1, and the concentration of magnetic beads is adjusted to 5 mg / ml. After this step is completed, the peristaltic pump stops and all the switch valves are closed.

[0168] Step A15: Elute the magnetic beads.

[0169] Turn off the magnetic field applied to the sorting column 101-1, open the first switch valve 103-1, the sixteenth switch valve 103-16, the fourteenth switch valve 103-14, the eighth switch valve 103-8 and the eighteenth switch valve 103-18, and operate the peristaltic pump to flush the immunomagnetic beads in the sorting column 101-1 into the destination bag at a certain flow rate to complete the preparation of the immunomagnetic beads.

[0170] In this embodiment, when the centrifuge bag 102 - 1 is about to overflow, the fifteenth switch valve 103 - 15 , the thirteenth switch valve 103 - 13 , the twelfth switch valve 103 - 12 and the nineteenth switch valve 103 - 19 can be opened to discharge directly into the waste liquid collection part 105 .

[0171] It should be noted that the structure of the immunomagnetic bead preparation system 100 is not limited to the following: Figure 1-Figure 2 The structure in can also be set to other structures, for example, Figure 3 and Figure 4 The structure shown, and Figure 1 and Figure 2 The structure is similar to that of the embodiment of the present invention, except that a twentieth switch valve 103-20, a twenty-first switch valve 103-21, a twenty-second switch valve 103-22 and a twenty-third switch valve 103-23 ​​and corresponding pipelines are added.

[0172] It should also be noted that, in this article, each containing member and collecting member can be a bag-shaped plastic product or other products.

[0173] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0174] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

[0175] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0176] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An immunomagnetic bead preparation system (100), characterized in that: include: Closed pipeline (101); A magnetic bead sample holding component connected to the sealed pipeline (101); an oxidizing liquid containing member connected to the sealed pipeline (101); a coating liquid containing member communicated with the sealed pipeline (101); A non-specific interface sealing liquid containing component connected to the sealed pipeline (101); A mixing element (102) connected to the sealed pipeline (101); A pipeline control component (103) is arranged on the closed pipeline (101), and the pipeline control component (103) is used to control the magnetic bead sample holding part, the oxidizing liquid holding part, the coating liquid holding part and the non-specific interface sealing liquid holding part to be connected with the mixing part (102) in sequence, so as to input the magnetic bead sample into the mixing part (102), input the oxidizing liquid into the mixing part (102) to oxidize the magnetic beads, input the coating liquid into the mixing part (102) to coat the oxidized magnetic beads, and input the non-specific interface sealing liquid into the mixing part (102) to seal the non-specific interface on the surface of the coated magnetic beads, so as to obtain immunomagnetic beads.

2. The immunomagnetic bead preparation system (100) according to claim 1, characterized in that: Also includes a power drive member (104); The power driving member (104) is installed on the sealed pipeline (101) and is used to drive the liquid in the sealed pipeline (101) to move along a first preset path of the sealed pipeline (101).

3. The immunomagnetic bead preparation system (100) according to claim 1, characterized in that: It also includes a waste liquid collection component (105) and a magnetic field loading and unloading mechanism (106); A separation column (101-1) is arranged on the closed pipeline (101), and the magnetic field loading and unloading mechanism (106) is used to load or unload the magnetic field on the separation column (101-1); The waste liquid collecting component (105) is in communication with the closed pipeline (101), and the pipeline control component (103) is also used to control the mixing component (102) and the waste liquid collecting component (105) to be in communication with the separation column (101-1) respectively; When the waste liquid discharged from the mixing element (102) needs to be discharged, the mixing element (102) and the waste liquid collecting element (105) are respectively connected to the separation column (101-1), and the magnetic field loading and unloading mechanism (106) loads the magnetic field to the separation column (101-1).

4. The immunomagnetic bead preparation system (100) according to claim 3, characterized in that: Also included is a destination collection piece (107); The target collecting piece (107) is in communication with the closed pipeline (101), and the pipeline control component (103) is also used to control the communication between the separation column (101-1) and the target collecting piece (107).

5. The immunomagnetic bead preparation system (100) according to claim 1, characterized in that: Also included is a cleaning liquid containing member (108); The cleaning liquid holding component (108) is connected to the closed pipeline (101), and the pipeline control component (103) is also used to control the cleaning liquid in the cleaning liquid holding component (108) to flow along a second preset path of the closed pipeline (101) to clean the closed pipeline (101) and / or various components connected to the closed pipeline (101).

6. The immunomagnetic bead preparation system (100) according to claim 5, characterized in that: The cleaning liquid containing part (108) comprises a buffer solution containing part and a saline solution containing part respectively connected to the sealed pipeline (101); The salt solution in the salt solution container can clean the oxidized magnetic beads.

7. The immunomagnetic bead preparation system (100) according to any one of claims 1 to 6, characterized in that: Also includes an airtightness detection component (109); The airtightness detection component (109) is installed on the sealed pipeline (101) and is used to detect the airtightness of the sealed pipeline (101).

8. The immunomagnetic bead preparation system (100) according to claim 2, characterized in that: The pipeline control component (103) comprises a plurality of switch valves clamped outside the closed pipeline (101); and / or, The power driving member (104) is a peristaltic pump clamped outside the closed pipeline (101); and / or, The mixing element (102) comprises a centrifuge and a centrifuge bag (102-1) arranged in the centrifuge, and the centrifuge bag is rotatable and connected to the closed pipeline (101).

9. A method for preparing immunomagnetic beads, characterized in that: include: Providing an immunomagnetic bead preparation system (100) as described in any one of claims 1 to 8; Connecting the magnetic bead sample holding part and the mixing part (102) in the immunomagnetic bead preparation system (100) to input the magnetic bead sample into the mixing part (102); connecting the oxidizing liquid containing part and the mixing part (102) in the immunomagnetic bead preparation system (100) and inputting the oxidizing liquid into the mixing part (102) to oxidize the magnetic beads; Connecting the coating liquid containing part and the mixing part (102) in the immunomagnetic bead preparation system (100), and inputting the coating liquid into the mixing part (102) to coat the oxidized magnetic beads; The non-specific interface blocking liquid storage part and the mixing part (102) in the immunomagnetic bead preparation system (100) are connected, and the non-specific interface blocking liquid is input into the mixing part (102) to block the non-specific interface on the surface of the coated magnetic beads, thereby obtaining the immunomagnetic beads.

10. The method for preparing immunomagnetic beads according to claim 9, characterized in that: After each component is input into the mixing element (102), the following steps are performed: washing the magnetic beads.

11. The method for preparing immunomagnetic beads according to claim 10, characterized in that: Washing the magnetic beads comprises: The magnetic field loading and unloading mechanism (106) in the immunomagnetic bead preparation system (100) is started to load the magnetic field to the sorting column (101-1) of the immunomagnetic bead preparation system (100), the mixing element (102) and the sorting column (101-1) are connected, the sorting column (101-1) and the waste liquid collection element (105) in the immunomagnetic bead preparation system (100) are connected, the magnetic beads are adsorbed into the sorting column (101-1), and the waste liquid enters the waste liquid collection element (105); The cleaning liquid holding part (108) and the mixing part (102) in the immunomagnetic bead preparation system (100) are connected, the mixing part (102) and the separation column (101-1) are rinsed with the cleaning liquid in the cleaning liquid holding part (108), and the waste liquid is discharged into the waste liquid collecting part (105); The separation column (101-1) is connected to the mixing element (102) or the target collecting element (107) in the immunomagnetic bead preparation system (100), and the magnetic field of the separation column (101-1) is unloaded by the magnetic field loading and unloading mechanism (106), so that the magnetic beads in the separation column (101-1) are transported to the mixing element (102) or the target collecting element (107).

12. The method for preparing immunomagnetic beads according to claim 10, characterized in that: When the oxidized magnetic beads are cleaned, the cleaning liquid holding part (108) in the immunomagnetic bead preparation system (100) includes a buffer solution holding part and a salt solution holding part, and connecting the cleaning liquid holding part (108) and the mixing part (102) includes: connecting the salt solution containing part and the mixing part (102) to flush the mixing part (102) and the separation column (101-1) with the salt solution; The buffer solution containing part and the mixing part (102) are connected, and the mixing part (102) and the separation column (101-1) are flushed with the buffer solution.

13. The method for preparing immunomagnetic beads according to claim 9, characterized in that: The magnetic bead sample holding part and the mixing part (102) in the immunomagnetic bead preparation system (100) are turned on, and the magnetic bead sample is input into the mixing part (102) specifically including: The magnetic field loading and unloading mechanism (106) in the immunomagnetic bead preparation system (100) is started to load a magnetic field on the sorting column (101-1) of the immunomagnetic bead preparation system (100), the magnetic bead sample holding part and the sorting column (101-1) are connected, and the sorting column (101-1) and the waste liquid collecting part (105) in the immunomagnetic bead preparation system (100) are connected, so that the magnetic beads output by the magnetic bead sample holding part are adsorbed in the sorting column (101-1), and the waste liquid is discharged into the waste liquid collecting part (105); Disconnecting the magnetic bead sample holding part from the separation column (101-1), connecting the buffer holding part in the immunomagnetic bead preparation system (100) and the separation column (101-1), so that the buffer in the buffer holding part flows through the separation column (101-1) to wash the magnetic beads, and is discharged into the waste liquid collection part (105); The separation column (101-1) and the waste liquid collecting part (105) are disconnected, and the separation column (101-1) and the mixing part (102) are connected, and the magnetic field of the separation column (101-1) is unloaded through the magnetic field loading and unloading mechanism (106), so that the buffer solution flows through the separation column (101-1) and transports the magnetic beads to the mixing part (102).

14. The method for preparing immunomagnetic beads according to any one of claims 9 to 13, characterized in that: Before inputting the magnetic bead sample into the magnetic bead sample holding member, the method further comprises: Detecting the air tightness of the immunomagnetic bead preparation system (100) by using the air tightness detection component (109) of the immunomagnetic bead preparation system (100); The immunomagnetic bead preparation system (100) is rinsed.

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