Antibacterial cleansing compositions, uses and methods

Abstract

An antibacterial cleansing composition comprising: from about 0.01% to about 1%, by weight of the composition, of chloroxylenol; from about 5% to about 15% sodium lauryl sulfate by weight of the composition; from about 1% to about 5% by weight of the composition of a co-surfactant, wherein the co-surfactant is selected from zwitterionic surfactants, amphoteric surfactants, nonionic surfactants, or combinations thereof; wherein the weight ratio of sodium lauryl sulfate to the cosurfactant is in the range of about 3: 1 to about 5: 1; and wherein the composition has a pH of from about 2 to about 5.

Description

Technical Field

[0001] The present application is generally directed to an antibacterial cleaning composition, uses and methods related thereto. The antibacterial cleaning composition comprises chloroxylenol; sodium lauryl sulfate and a co-surfactant; wherein the weight ratio of sodium lauryl sulfate to the co-surfactant is in the range of about 3:1 to about 5:1; and wherein the composition has a pH of about 2 to about 5. Background Art

[0002] Human health is affected by many microbial entities or microorganisms, such as germs, bacteria, fungi, yeasts, molds, viruses, etc. For example, infection with microbial entities or microorganisms including various viruses and bacteria leads to various diseases and ailments. In order to reduce such infections, people usually wash their skin with cleaning compositions. Cleaning compositions are traditionally sold in a variety of forms, such as bar soaps, creams, lotions, and gels.

[0003] Washing with a cleansing composition, such as an antimicrobial soap, removes dirt and germs from the skin. The removal of viruses and bacteria is due to the surfactant properties of the soap and the mechanical action of the washing process. Antimicrobial soaps typically include soap combined with one or more active substances, such as antimicrobial agents; which may be in the form of a soap bar. When washing the skin with an antimicrobial soap, such as a bar soap, the surfactant of the soap typically removes most of the microbial entities or microorganisms on the skin, while the antimicrobial agent is at least partially deposited on the skin to provide residual protection against subsequent invasion.

[0004] Bacteria found on the skin can be divided into two groups: resident bacteria and transient bacteria. Resident bacteria are Gram-positive bacteria that establish as permanent microcolonies on the surface and outermost layers of the skin and play a relevant, helpful role in preventing colonization by other, more harmful bacteria and fungi.

[0005] Transient bacteria are bacteria that are not part of the normal resident flora of the skin, but can be deposited when airborne contaminated material lands on the skin or when contaminated material comes into physical contact with the skin. Transient bacteria are generally divided into two subtypes: Gram-positive bacteria and Gram-negative bacteria. Gram-positive bacteria include pathogens such as Staphylococcus aureus. Gram-negative bacteria include pathogens such as Escherichia coli. Gram-negative bacteria are generally distinguished from Gram-positive bacteria by an additional protective cell membrane, which generally renders Gram-negative bacteria less susceptible to topical antimicrobial active substances.

[0006] Given the health implications of bacteria such as S. aureus, there remains a need to formulate antimicrobial cleansing compositions that provide improved germ reduction on the skin.

[0007] Cleaning compositions are traditionally sold in a variety of forms, such as bar soaps, creams, lotions, and gels. Typically, these products need to meet a number of criteria acceptable to consumers, such as acceptable viscosity to deliver the active substance of the composition. These criteria include cleaning effect, skin feel, mildness to the skin, hair, and ocular mucosa, and foam volume. An ideal cleanser should gently clean the skin or hair, cause little or no irritation, and should not overdry the skin or hair after frequent use.

[0008] Therefore, there remains a need to formulate antimicrobial cleansing compositions that have adequate rheology that is acceptable to consumers and that delivers the actives of the composition. Summary of the invention

[0009] An antibacterial cleaning composition is provided, comprising:

[0010] a) from about 0.01% to about 1%, by weight of the composition, of chloroxylenol;

[0011] b) from about 5% to about 15%, by weight of the composition, of sodium lauryl sulfate;

[0012] c) from about 1% to about 5% by weight of the composition of a co-surfactant, wherein the co-surfactant is selected from a zwitterionic surfactant, an amphoteric surfactant, a nonionic surfactant, or a combination thereof; d) wherein the weight ratio of sodium lauryl sulfate to the co-surfactant is in the range of from about 3:1 to about 5:1, preferably from about 7.5:2.5 to about 8:2; and wherein the composition has a pH of from about 2 to about 5.

[0013] A method for washing hands and removing about 99% of germs is provided, the method comprising the following steps in order:

[0014] a) providing a container suitable for containing the antimicrobial cleaning composition described herein;

[0015] b) dispensing the antibacterial cleansing composition into the palm of a user;

[0016] c) optionally, lather by rubbing your palms together;

[0017] d) spreading the antibacterial cleansing composition on the palms, backs of hands, between fingers and / or under nails;

[0018] e) Wash your hands;

[0019] f) optionally drying;

[0020] g) wherein the antibacterial cleaning composition removes about 99% of bacteria.

[0021] Provided is a liquid hand washing composition, comprising:

[0022] a) from about 7% to about 9%, preferably from about 7.5% to about 8%, by weight of the composition, of sodium lauryl sulfate;

[0023] b) from about 1.75% to about 3.0%, preferably from about 2.00% to about 2.5%, by weight of the composition, of cocamidopropyl betaine;

[0024] c) wherein the weight ratio of sodium lauryl sulfate to cocamidopropyl betaine is in the range of about 7.5:2.5 to about 8:2;

[0025] d) from about 0.40% to about 0.60%, by weight of the composition, of chloroxylenol; and

[0026] wherein the composition has a pH of about 4 to about 5 and a viscosity of about 3,000 cps to about 25,000 cps. DETAILED DESCRIPTION

[0027] Definition of terms

[0028] In this document, unless specifically stated otherwise, the following definitions apply in all embodiments, including in all aspects of the invention.

[0029] Unless otherwise specified, all percentages are by weight (w / w) of the composition. "wt. %" means weight percentage. References to 'parts' (e.g., a mixture of 1 part X and 3 parts Y) are ratios by weight. Unless otherwise specifically stated, all ratios or percentages are weight ratios or weight percentages.

[0030] An "active composition" is a composition that does not contain water, and an "active ingredient" is an ingredient that does not contain water.

[0031] "QS" or "QSP" means sufficient for 100% or for 100 g. + / - indicates standard deviation. All ranges are inclusive and combinable. The number of significant figures expresses neither a limitation on the amount shown nor a limitation on the accuracy of the measurement. All numerical values ​​are understood to be modified by the word "about".

[0032] Unless otherwise indicated, all measurements are understood to be made at 25°C and ambient conditions, where "ambient conditions" means at a pressure of 1 atmosphere (atm) and at 65% relative humidity. "Relative humidity" means the ratio of the moisture content of air compared to the saturation moisture content at the same temperature and pressure (expressed as a percentage). Relative humidity can be measured using a hygrometer, specifically using International probe moisture meter.

[0033] As used herein, "min" means "one minute" or "several minutes". As used herein, "mol" means "mole". As used herein, "g" after a number means "gram". "Ex." means "Example". All amounts referring to listed ingredients are based on the active content and do not include carriers or by-products that may be included in commercially available materials.

[0034] As used herein, "comprising" means that other steps and other ingredients may be added. "Comprising" encompasses the terms "consisting of" and "consisting essentially of". The compositions, methods, uses and processes of the present invention may include, consist of and consist essentially of the elements and limitations of the present invention described herein, as well as any additional or optional ingredients, components, steps or limitations described herein. Unless incompatibility is indicated, the embodiments and aspects described herein may include elements, features or components of other embodiments and / or aspects or may be combined with elements, features or components of other embodiments and / or aspects, although not explicitly exemplified in the combination.

[0035] As used herein, articles including "a" and "an" when used in a claim should be understood to mean one or more of what is claimed or described.

[0036] As used herein, the terms "including," "comprising," and "containing" are intended to be non-limiting.

[0037] Where content ranges are given, these should be understood to be the total amount of the ingredient in the composition, or where more than one material falls within an ingredient definition, the total amount of all ingredients in the composition that meet the definition.

[0038] For example, if a composition comprises 1% to 5% fatty alcohol, a composition comprising 2% stearyl alcohol and 1% cetyl alcohol and no other fatty alcohols would fall within the range.

[0039] The amount of each specific ingredient or mixtures thereof described below may comprise up to 100% (or 100%) of the total amount of ingredients in the composition.

[0040] As used herein, the term "free of" means that the composition comprises 0% of an ingredient by weight of the composition and thus has no detectable amount of the ingredient.

[0041] As used herein, the term "substantially free" means less than about 1%, less than about 0.8%, less than about 0.5%, less than about 0.3%, or less than an insubstantial amount by weight of the composition.

[0042] Herein, "Comp. Ex." or "C. Ex." means "Comparative Example"; and "Ex." means "Example".

[0043] Unless otherwise indicated, as used herein, the term "molecular weight" or "M.Wt." refers to weight average molecular weight. Weight average molecular weight can be measured by gel permeation chromatography ("GPC").

[0044] As used herein, the term "cleansing composition" refers to compositions intended for topical application to the skin for cleansing.

[0045] As used herein, the term "fragrance" refers to a mixture of volatile organic oils having a pleasing aroma, wherein the fragrance components have individual molecular weights between about 75 and 400 Daltons.

[0046] As used herein, the term "mixture" is meant to include a simple combination of substances, as well as any compounds that may result from their combination.

[0047] The term "room temperature" refers to a temperature of 25°C.

[0048] As used herein, the term "rinse-off" means that the intended use of the product includes application to the skin and / or hair, and then the product is washed and / or wiped off the skin and / or hair within seconds to minutes of the application step. The product is usually applied and rinsed in the same use event, such as showering or washing hands.

[0049] As used herein, the term "derivative" refers to a structure not shown but understood by one skilled in the art to be a variation of the basic compound.

[0050]

[0043] The terms "apply" or "applying" as used with reference to a composition means applying or spreading the composition of the present invention onto the skin.

[0051] The term "dermatologically acceptable" means that the compositions or components described are suitable for use in contact with human skin tissue without undue toxicity, incompatibility, instability, allergic response, and the like.

[0052] As used herein, the term "cosmetically acceptable" means that the composition, formulation or component described is suitable for use in contact with human keratinous tissue without undue toxicity, incompatibility, instability, allergic response, etc. All compositions described herein intended for direct application to keratinous tissue are limited to those that are cosmetically acceptable.

[0053] Unless specifically stated otherwise, the methods as disclosed herein are cosmetic methods or non-therapeutic methods.

[0054] The object of the present invention is to provide an antibacterial cleaning composition, method and use of the product, structure and corresponding composition as described in the summary of the invention or as described below for achieving the technical effects or goals as set forth herein. These objectives and other advantages described in the above summary of the invention and the detailed description and defined in the subsequent claims that can be obtained by the present invention may be obvious to those skilled in the art.

[0055] Beneficial Effects

[0056] Human skin is the largest organ of the body and is part of the body's natural defense against microbial attack. Natural skin defense consists of multiple components. For example, the skin's natural defense includes the skin's ability to act as a physical barrier, the skin's pH, the skin's microecology, the lipids on the skin, the skin's chemical composition, etc.

[0057] The ability of human skin to provide a natural defense against microbial attack can be affected by what it comes into contact with throughout the day, such as skin care products. Bacteria not only stay on the surface of the skin, but also penetrate deep into the skin. It damages the skin barrier, causing skin sensitivity and irritation.

[0058] It was found that antimicrobial cleaning compositions containing chloroxylenol, or a mixture of chloroxylenol and pyrrolidone carboxylic acid, can provide consistent antimicrobial efficacy as shown in the standard time kill study described herein. Using the antimicrobial cleaning compositions as disclosed herein, immediate germ kill efficacy has been found at 30 and 60 second contact times.

[0059] In addition, in order to achieve antibacterial properties, the antibacterial cleaning composition needs to meet optimized rheological properties. Surprisingly, the specific weight ratio of sodium lauryl sulfate to co-surfactant can directly affect the viscosity of the antibacterial cleaning composition and help deliver the antibacterial active substance to the skin. When the weight ratio of sodium lauryl sulfate to co-surfactant is in the range of about 3:1 to about 5:1, preferably about 7.5:2.5 to about 8:2, the rheological properties are sufficiently acceptable so that the antibacterial active substance can be delivered to the skin. Compositions that are relatively too thin can be prevented, which is also preferred by consumers.

[0060] Antibacterial cleaning composition

[0061] Antimicrobial active substances

[0062] The antimicrobial cleaning composition comprises from about 0.01% to about 1%, preferably from about 0.10% to about 0.75%, more preferably from about 0.40% to about 0.60%, by weight of the composition, of chloroxylenol (also known as 4-chloro-3,5-dimethylphenol or para-chloro-meta-xylenol or PCMX).

[0063] Additionally, the antibacterial cleaning composition may comprise from about 0.005% to about 5%, preferably from about 0.02% to about 1%, more preferably from about 0.025% to about 0.15%, even more preferably from about 0.025% to about 0.05%, most preferably from about 0.025% to about 0.035%, by weight of the composition, of pyrrolidonecarboxylic acid (also known as 2-pyrrolidone-5-carboxylic acid or pyridinic acid).

[0064] Without wishing to be bound by theory, chloroxylenol alone, or in combination with pyrrolidone carboxylic acid, may provide consistent antimicrobial efficacy, particularly against S. aureus and / or any of the microorganisms listed in Table 1 as listed below in the Test Methods section.

[0065] Pyrrolidone carboxylic acid can also protonate the carboxylate functional groups on the phospholipid membrane of bacteria and reduce the tendency of the membrane to electronically repel anionic surfactants, thereby promoting the proper interaction between the anionic surfactants of the present invention and the membrane.

[0066] Furthermore, pyrrolidone carboxylic acid can help promote the creation of a low pH buffer on the surface of the substrate, thereby helping human skin maintain its natural barrier to bacteria.

[0067] Surfactants

[0068] The antibacterial cleaning composition comprises: about 5% to about 15% of sodium lauryl sulfate by weight of the composition; about 1% to about 5% of an auxiliary surfactant by weight of the composition, wherein the auxiliary surfactant is selected from a zwitterionic surfactant, an amphoteric surfactant, a nonionic surfactant, or a combination thereof; wherein the weight ratio of sodium lauryl sulfate to the auxiliary surfactant is in the range of about 3:1 to about 5:1, preferably about 7.5:2.5 to about 8:2.

[0069] The antimicrobial cleaning composition comprises from about 5% to about 15%, preferably from about 7% to about 9%, more preferably from about 7.5% to about 8%, by weight of the composition, of sodium lauryl sulfate.

[0070] In a specific aspect, the antibacterial cleaning composition can comprise from about 7% to about 9%, preferably from about 7.5% to about 8%, by weight of the composition, of sodium lauryl sulfate. In this specific aspect, the antibacterial cleaning composition is a liquid hand detergent composition.

[0071] The antibacterial cleaning composition comprises from about 1% to about 5%, preferably from about 1.4% to about 3.0%, more preferably from about 2% to about 2.5% of a co-surfactant by weight of the composition. The co-surfactant is selected from the group consisting of zwitterionic surfactants, amphoteric surfactants, nonionic surfactants, and mixtures thereof.

[0072] Sodium lauryl sulfate and co-surfactants as described herein can provide cleaning benefits, foaming properties, and rheological properties to the composition.

[0073] The antimicrobial cleaning composition may include at least one of an amphoteric surfactant and a zwitterionic surfactant. Suitable amphoteric surfactants or zwitterionic surfactants may include those described in U.S. Pat. No. 5,104,646 and U.S. Pat. No. 5,106,609.

[0074] Amphoteric surfactants may include those broadly described as derivatives of aliphatic secondary and tertiary amines, wherein the aliphatic radical may be straight or branched chain, and wherein the aliphatic substituent may contain from 8 to 18 carbon atoms, such that one carbon atom may contain a water-solubilizing anionic group, such as a carboxyl, sulfonate, sulfate, phosphate, or phosphonate. Examples of compounds falling within this definition may be sodium 3-dodecyl-aminopropionate, sodium 3-dodecylaminopropane sulfonate, sodium lauryl sarcosinate, N-alkyltaurines (such as those prepared by reacting dodecylamine with sodium isethionate according to the teachings of U.S. Pat. No. 2,658,072), N-higher alkyl aspartic acids (such as those prepared according to the teachings of U.S. Pat. No. 2,438,091), and the products described in U.S. Pat. No. 2,528,378.

[0075] Alternatively or in addition, the auxiliary surfactant included in the antibacterial cleaning compositions described herein may include an amphoteric surfactant selected from the group consisting of sodium lauroamphoacetate, sodium cocoamphoacetate, disodium lauroamphoacetate, disodium cocodiamphoacetate, and mixtures thereof.

[0076] Zwitterionic surfactants suitable for use in the auxiliary surfactants of the antimicrobial cleaning compositions described herein may include those broadly described as derivatives of aliphatic quaternary ammonium, phosphonium and sulfonium compounds, wherein the aliphatic radical may be linear or branched, and wherein one of the aliphatic substituents may contain 8 to 18 carbon atoms, and one of the aliphatic substituents may contain an anionic group such as carboxyl, sulfonate, sulfate, phosphate or phosphonate. Zwitterionic surfactants included in the antimicrobial cleaning compositions described herein may include one or more betaines, including cocamidopropyl betaine.

[0077] Alternatively, the amphoteric or zwitterionic surfactant may be selected from cocamidopropyl betaine, lauramidopropyl betaine, cocobetaine, lauryl betaine, cocoamphoacetate, cocoamphodiacetate, lauroamphoacetate, lauroamphodiacetate, lauramine oxide, and mixtures thereof.

[0078] Examples of betaine zwitterionic surfactants may include cocodimethyl carboxymethyl betaine, cocoamidopropyl betaine (CAPB), cocobetaine, lauroamidopropyl betaine (LAPB), oleyl betaine, lauryl dimethyl carboxymethyl betaine, lauryl dimethyl α-carboxyethyl betaine, cetyl dimethyl carboxymethyl betaine, lauryl di-(2-hydroxyethyl) carboxymethyl betaine, stearyl di-(2-hydroxypropyl) carboxymethyl betaine, oleyl dimethyl γ-carboxypropyl betaine, lauryl di-(2-hydroxypropyl) α-carboxyethyl betaine, and mixtures thereof. Examples of sulfobetaines may include cocodimethyl sulfopropyl betaine, stearyl dimethyl sulfopropyl betaine, lauryl dimethyl sulfoethyl betaine, lauryl di-(2-hydroxyethyl) sulfopropyl betaine, and mixtures thereof.

[0079] The auxiliary surfactant may comprise a zwitterionic surfactant, preferably a betaine and / or a sultaine, more preferably a zwitterionic surfactant selected from the group consisting of cocamidopropyl betaine, lauroamidopropyl betaine, cocamidopropyl hydroxysultaine and mixtures thereof, even more preferably cocamidopropyl hydroxysultaine or cocamidopropyl betaine.

[0080] Preferably, the co-surfactant comprises cocamidopropyl betaine.

[0081] Suitable nonionic surfactants may include those selected from the group consisting of alkyl ethoxylates, alkyl glucosides, polyglucosides (e.g., alkyl polyglucosides, decyl polyglucosides), polyhydroxy fatty acid amides, alkoxylated fatty acid esters, sucrose esters, amine oxides, or mixtures thereof. Some exemplary nonionic surfactants may include cocamide monoethanolamine, decyl glucoside, or combinations thereof.

[0082] Additional surfactants and other ingredients suitable for use in antibacterial cleaning compositions are found in U.S. Publication No. 2020 / 0046623, which is incorporated herein by reference.

[0083] The antimicrobial cleaning composition has a weight ratio of sodium lauryl sulfate to co-surfactant in the range of about 3:1 to about 5:1, preferably about 7.5:2.5 to about 8:2, more preferably about 8:2.

[0084] Preferably, the auxiliary surfactant may be cocamidopropyl betaine, and the antibacterial cleaning composition has a weight ratio of sodium lauryl sulfate to cocamidopropyl betaine in the range of about 3:1 to about 5:1, preferably about 7.5:2.5 to about 8:2, more preferably about 8:2.

[0085] The antibacterial cleansing composition needs to achieve an optimized rheological property in order to deliver the antibacterial property of the composition to the user's skin. Surprisingly, a specific weight ratio of sodium lauryl sulfate to co-surfactant can directly affect the viscosity of the antibacterial cleansing composition. When the weight ratio of sodium lauryl sulfate to co-surfactant is in the range of about 3:1 to about 5:1, preferably about 7.5:2.5 to about 8:2, the rheological property is sufficiently acceptable to consumers so that the antibacterial active can be delivered to the skin.

[0086] The viscosity of the antibacterial cleaning composition may be from about 2.7 Pa.s (2,750 cps) to about 30 Pa.s (30,000 cps); preferably from about 3.0 Pa.s (3,000 cps) to about 25 Pa.s (25,000 cps); more preferably from about 3.4 Pa.s (3,400 cps) to about 24.25 Pa.s (24,250 cps).

[0087] pH

[0088] The pH of the antimicrobial cleaning composition is preferably from about 2.0 to about 5.0, more preferably from about 3.0 to about 3.5, even more preferably from about 3.1 to about 3.3.

[0089] The pH of the skin is usually naturally acidic, i.e., between about 4.5 and about 6.5. Acidic skin pH can help activate specific enzymes to regulate skin activity and balance the acidic mantle of the skin. This balanced acidic mantle of the skin, together with antimicrobial peptides (AMPs) present in the skin layers, maintains the skin's natural barrier against bacteria.

[0090] The antibacterial cleansing composition may have an acidic pH as defined above to help the skin strengthen its natural barrier against bacteria. In addition, the acidic pH may help promote the antibacterial properties of chloroxylenol; and, if added, may help promote the antibacterial properties of pyrrolidone carboxylic acid.

[0091] A variety of compounds can be used to adjust the pH of the composition. Such suitable compounds may include, but are not limited to, acetic acid, hydrochloric acid, sodium hydroxide, magnesium hydroxide, triethylamine, diethylamine, ethylamine, monoethanolamine, and any combination thereof. The antimicrobial cleaning composition may comprise greater than about 0% to about 2% of a pH adjuster by weight of the composition, preferably wherein the pH adjuster comprises citric acid.

[0092] Solubilizer

[0093] The antimicrobial composition may include one or more solubilizing agents, such as sugar alcohols or glycols. Sugar alcohols may include sorbitol. Glycols may include propylene glycol, dipropylene glycol, polyethylene glycol, their derivatives, and mixtures thereof. In one example, the antimicrobial cleaning composition may minimize the amount of solubilizing agent.

[0094] The antibacterial cleaning composition may contain no more than about 10%, alternatively no more than about 5%, alternatively no more than about 3%, alternatively no more than about 1%, preferably greater than 0% but less than about 3%, more preferably greater than 0% but less than about 1% of one or more solubilizing agents by weight of the composition. The antibacterial cleaning composition may be substantially free of solubilizing agents, alternatively, the composition may be free of solubilizing agents.

[0095] Structuring agent

[0096] The antibacterial cleaning composition may include one or more hydrophilic structurants, such as carbohydrate structurants and gums. Some suitable carbohydrate structurants include raw starch (corn, rice, potato, wheat, etc.) and pregelatinized starch. Some suitable gums include carrageenan, guar gum and xanthan gum. The antibacterial cleaning composition may include one or more carbohydrate structurants in an amount of about 0.1% to about 30%, about 2% to about 25%, or about 4% to about 20% by weight of the composition.

[0097] Wetting agent

[0098] The antibacterial cleaning composition may include one or more wetting agents. Examples of wetting agents may include polyols. In addition, wetting agents such as glycerol may be included in the antibacterial cleaning composition due to preparation or as an additional ingredient. For example, glycerol may be a byproduct of saponification of the cleaning composition. Including additional wetting agents may result in many beneficial effects, such as improving the hardness of the antibacterial cleaning composition, reducing the water activity of the antibacterial cleaning composition, and reducing the weight loss rate of the cleaning composition over time due to water evaporation. The antibacterial cleaning composition may include one or more wetting agents greater than about 0% to about 10% by weight of the composition.

[0099] Inorganic salt

[0100] The antibacterial cleaning composition may include one or more inorganic salts. The inorganic salts may help maintain a specific water content or level of the composition and improve the hardness of the composition. The inorganic salts may also help bind the water in the composition to prevent water loss due to evaporation or other means. The antibacterial cleaning composition may optionally include 0.1% to about 15%, preferably about 1% to about 12%, and more preferably about 2.5% to about 10.5% of one or more inorganic salts by weight of the composition. Examples of suitable inorganic salts may include magnesium nitrate, trimagnesium phosphate, calcium chloride, sodium carbonate, sodium aluminum sulfate, disodium phosphate, sodium polymetaphosphate, sodium magnesium succinate, sodium tripolyphosphate, aluminum sulfate, aluminum chloride, aluminum chlorohydrate, hydrated aluminum zirconium trichloride, hydrated aluminum zirconium trichloride glycine complex, zinc sulfate, ammonium chloride, ammonium phosphate, calcium acetate, calcium nitrate, calcium phosphate, calcium sulfate, iron sulfate, magnesium chloride, magnesium sulfate, and tetrasodium pyrophosphate.

[0101] Alternatively, the antimicrobial cleaning composition may not contain any inorganic salts.

[0102] Skin Benefit Agents

[0103] The antimicrobial cleansing composition may comprise from about 0.5% to about 20%, by weight of the composition, of one or more skin benefit agents.

[0104] Examples of suitable skin benefit agents may include petrolatum, glyceryl oleate, mineral oil, natural oils, and mixtures thereof.Additional examples of skin benefit agents may include water-insoluble or hydrophobic benefit agents.

[0105] Non-limiting examples of glycerides suitable for use as hydrophobic skin benefit agents herein can include castor oil, safflower oil, corn oil, walnut oil, peanut oil, olive oil, cod liver oil, almond oil, avocado oil, palm oil, sesame oil, vegetable oils, sunflower oil, soybean oil, vegetable oil derivatives, coconut oil and derivatized coconut oils, cottonseed oil and derivatized cottonseed oils, jojoba oil, cocoa butter, and mixtures thereof.

[0106] Non-limiting examples of alkyl esters suitable for use as hydrophobic skin benefit agents herein may include isopropyl fatty acid esters and long chain esters of long chain (e.g., C10-C24) fatty acids (e.g., cetyl ricinoleate), non-limiting examples of which may include isopropyl palmitate, isopropyl myristate, cetyl ricinoleate, and octadecyl ricinoleate. Other examples may include hexyl laurate, isohexyl laurate, myristyl myristate, isohexyl palmitate, decyl oleate, isodecyl oleate, hexadecyl stearate, decyl stearate, isopropyl isostearate, diisopropyl adipate, diisohexyl adipate, dihexyldecyl adipate, diisopropyl sebacate, acyl isononanoate lauryl lactate, myristyl lactate, cetyl lactate, and mixtures thereof.

[0107] Non-limiting examples of polyglyceryl fatty acid esters suitable for use as hydrophobic skin benefit agents herein can include decaglyceryl distearate, decaglyceryl diisostearate, decaglyceryl monomyristate, decaglyceryl monolaurate, hexaglyceryl monooleate, and mixtures thereof.

[0108] preservative

[0109] The antibacterial cleaning composition may include one or more preservatives, which are generally less than about 2% by weight of the composition, preferably 0.1% to 0.5% by weight of the composition. Such suitable preservatives may include, but are not limited to, benzyl alcohol, kathon, propylene glycol, hydroxyacetophenone, sodium benzoate, disodium ethylenediaminetetraacetic acid (EDTA), parabens, phenoxyethanol, imidazolidinyl urea, and any mixture thereof.

[0110] The one or more preservatives may preferably include sodium benzoate, or a mixture of sodium benzoate and kasone. The kasone is composed of methylchloroisothiazolinone and methylisothiazolinone.

[0111] Alternatively, the one or more preservatives may preferably include salicylic acid, sodium benzoate, benzyl alcohol and kasone. Kasone consists of methylchloroisothiazolinone and methylisothiazolinone. In this aspect or in any aspect, the one or more preservatives may include a mixture of salicylic acid and sodium benzoate, wherein the total amount of salicylic acid and sodium benzoate is from about 0.2% to about 0.9%, preferably from about 0.5% to about 0.85%, more preferably from about 0.75% to about 0.85% by weight of the composition.

[0112] The weight ratio of salicylic acid to sodium benzoate may be about 1:1.25 to about 1:1.10, preferably about 1:1.175 to about 1:1.125.

[0113] Optional Ingredients

[0114] As can be appreciated, cleaning compositions as described herein can include a variety of optional components to adjust the properties and characteristics of the composition. As can be appreciated, suitable optional components are well known and can generally include any component that is physically and chemically compatible with the essential components of the composition as described herein. The optional components should not otherwise unduly impair the stability, aesthetics or performance of the product. The independent concentration of the optional components can be generally in the range of about 0.001% to about 10% by weight of the composition. The optional components can be further limited to components that will not impair the transparency of the translucent composition.

[0115] Optional components may include, but are not limited to, conditioning agents (including hydrocarbon oils, fatty acid esters, silicones), cationic polymers, anti-dandruff actives, and chelating agents. Additional suitable optional ingredients include, but are not limited to, colorants, particles, antimicrobial agents, foam boosters, antistatic agents, humectants, propellants, self-foaming agents, pH adjusters and buffers, preservatives, pearlescent agents, opacifiers, sensates, suspending agents, solvents, diluents, antioxidants, vitamins, and mixtures thereof.

[0116] Such optional ingredients should be physically and chemically compatible with the components of the composition and should not otherwise unduly impair product stability, aesthetics or performance. The CTFA Cosmetic Ingredient Handbook, Tenth Edition (published by the Cosmetic, Toiletry, and Fragrance Association, Inc. (Washington, DC)) (2004) (hereinafter "CTFA") describes a wide variety of non-limiting materials that can be added to the compositions herein.

[0117] Fragrance ingredients

[0118] The antibacterial cleaning composition may comprise from about 0.1 wt % to about 10 wt % of a fragrance component, preferably from about 0.2 wt % to about 0.7 wt % of a fragrance component, more preferably from 0.2 wt % to 0.6 wt % of a fragrance component, based on the weight of the composition.

[0119] Alternatively, the antimicrobial cleaning composition may not include any fragrance component.

[0120] Typically, fragrance components can be a blend of perfumes and aroma chemicals.As used herein, "fragrance" is used to refer to any odor-emitting material.

[0121] A variety of chemicals are known as fragrances, including alcohols, aldehydes, ketones and esters. Non-limiting examples of fragrances that can be used herein include fragrance precursors, such as acetal fragrance precursors, ketal fragrance precursors, ester fragrance precursors, hydrolyzable inorganic-organic fragrance precursors and mixtures thereof. Fragrances can be released from fragrance precursors in a variety of ways. For example, fragrances can be released as a result of simple hydrolysis, or by moving in an equilibrium reaction, or by pH-changes, or by enzyme release. Fragrances herein can be relatively simple in their chemical composition, comprising a single chemical, or can comprise a highly complex composite mixture of natural and synthetic chemical components, all of which are selected to provide any desired odor.

[0122] The fragrance may have a boiling point (BP) of 500° C. or less, 400° C. or less, or 350° C. or less. The BP of many fragrances is disclosed in Perfume and Flavor Chemicals, Steffen Arctander (1969). The ClogP value of a fragrance may be 0.1 or greater, 0.5 or greater, 1.0 or greater, and 1.2 or greater. As used herein, "ClogP" refers to the logarithm of the octanol / water partition coefficient to the base 10. ClogP can be easily calculated by a program called "CLOGP", which can be obtained from Daylight Chemical Information Systems Inc., Irvine Calif., USA. The octanol / water partition coefficient is described in more detail in U.S. Patent No. 5,578,563.

[0123] Suitable fragrances are also disclosed in US Pat. No. 4,145,184, US Pat. No. 4,209,417, US Pat. No. 4,515,705, and US Pat. No. 4,152,272. Non-limiting examples of fragrances include animal fragrances such as musk oil, civet, castoreum, ambergris, plant fragrances such as nutmeg extract, cardamom extract, ginger extract, cinnamon extract, patchouli oil, geranium oil, orange oil, tangerine oil, orange Hower extract, cedar, vetiver, lavender, ylang-ylang extract, tuberose extract, sandalwood oil, bergamot oil, rosemary oil, spearmint oil, peppermint oil, lemon oil, lavender oil, citronella oil, chamomile oil, clove oil, sage oil, neroli oil, labdanum oil, eucalyptus oil, verbena oil, mimosa extract, narcissus extract, carrot seed extract, jasmine extract, frankincense extract, rose extract, and mixtures thereof.

[0124] Other examples of suitable fragrances include, but are not limited to, chemicals such as acetophenone, adoxaldehyde, aldehyde C-12, aldehyde C-14, aldehyde C-18, allyl octanoate, ambroxol, amyl acetate, dimethyl indane derivatives, alpha-amyl cinnamaldehyde, anethole, anisaldehyde, benzaldehyde, borneol, butyl acetate, camphor, carbitol, cinnamaldehyde, cinnamyl acetate, cinnamyl alcohol, cis-3-hexanol and ester derivatives, cis-3-bexenyl methyl carbonate, citral, citronellol and ester derivatives, cuminaldehyde, cyclamen aldehyde, cyclopentyl, damascenone, decalactone, decanol, grass Artemisia annua, dihydromyrcenol, dimethylbenzyl alcohol, 6,8-dimethyl-2-nonanol, dimethylbenzyl butyrate, ethyl acetate, ethyl isobutyrate, ethyl butyrate, ethyl propionate, ethyl octanoate, ethyl cinnamate, ethyl caproate, ethyl valerate, ethyl vanillin, eugenol, cyclopentadecalactone, fenchone, fruity esters such as ethyl 2-methylbutyrate, galaxol, geraniol and ester derivatives, neoheterol, 2-heptanone, hexenol, α-hexylcinnamaldehyde, hydroxycitronellal, indole, isoamyl acetate, isoeugenyl acetate, ionone, isoeugenol, isoamyl isovalerate, isoE super, limonene, linalool, lily of the valley aldehyde, linalool acetate, lyral, maltol, mayol, melon aldehyde, menthol, p-methylacetophenone, methyl anthranilate, methyl cedryl ketone, methyl dihydrojasmonate, methyl eugenol, methyl ionone, methyl-α-naphthyl ketone, methyl phenyl acetate, mugetanol, γ-nonalactone, octanal, phenylethyl acetate, phenylacetaldehyde dimethyl acetate, phenoxyethyl isobutyrate, phenylethyl alcohol, pinene, sandalwood, santaiol, oleagin, thymol, terpenes, privet aldehyde, triethyl citrate, 3,3,5-trimethylcyclohexanol, γ-undecalactone, undecenal, vanillin, vanolone, verdox, and mixtures thereof.

[0125] In the antibacterial cleaning composition, the fragrance component may include ketone and / or aldehyde fragrance components. In addition, the fragrance component may also include any fragrance described above.

[0126] The ketone fragrance component may be selected from alicyclic ketones such as β-ionone, terpene ketones such as 1-carvone, and macrocyclic ketones such as cyclopentadecanone.

[0127] The aldehyde fragrance component may be selected from aliphatic aldehydes such as 2,6-nonadienal, terpene aldehydes such as citral, and aromatic aldehydes such as α-hexylcinnamaldehyde, cinnamaldehyde.

[0128] Therefore, preferably, the fragrance component may include a ketone and / or aldehyde fragrance component, wherein the ketone and / or aldehyde fragrance component may be selected from the group consisting of acetophenone, adoc aldehyde, aldehyde C-12, aldehyde C-14, aldehyde C-18, α-amyl cinnamaldehyde, anise aldehyde, benzaldehyde, camphor, cinnamaldehyde, citral, cuminaldehyde, cyclamen aldehyde, damascenone, fenchone, heliotropin, 2-heptanone, α-hexyl cinnamaldehyde, hydroxycitronellal, ionone, lilyral, lyral, melonal, p-methylacetophenone, methyl cedryl ketone, methyl ionone, methyl-α-naphthyl ketone, γ-nonalactone, octanal, phenylacetaldehyde, dimethyl acetate, privet aldehyde, γ-undecalactone, undecenal, vanillin, vanillone, and mixtures thereof.

[0129] Rinse-off cleaning composition

[0130] The antimicrobial cleaning composition may be a rinse-off cleaning composition. The rinse-off cleaning composition may have a variety of forms. For example, the rinse-off cleaning composition may be in liquid form and may be a body wash, shampoo, conditioning shampoo, moisturizing body wash, shower gel, skin cleanser, cleansing milk, bath body cream, pet shampoo, shaving preparation, etc. The rinse-off cleaning composition may be a hand wash or bath composition, such as a liquid hand wash or a foaming hand wash composition.

[0131] The rinse-off cleaning composition can be water-based. It should be understood that during the process of preparing the rinse-off cleaning composition, the amount of water may be lost, i.e. evaporated, or subsequently the water is absorbed by surrounding packaging materials (e.g., cardboard boxes), etc. Therefore, the rinse-off cleaning composition can also include materials that tend to bind water so that water is maintained in the cleaning composition at a desired level. The example of such material can include carbohydrate structurants and wetting agents such as glycerol. The rinse-off cleaning composition can include water in an amount exceeding about 80%, alternatively exceeding about 85%, alternatively exceeding about 88% by the gross weight of the composition. However, it should be understood that the rinse-off cleaning composition can be anhydrous.

[0132] Liquid hand detergent

[0133] Provided is a liquid hand washing composition, comprising:

[0134] a) from about 7% to about 9%, preferably from about 7.5% to about 8%, by weight of the composition, of sodium lauryl sulfate;

[0135] b) from about 1.75% to about 3%, from about 2% to about 2.5%, by weight of the composition, of cocamidopropyl betaine, wherein the weight ratio of sodium lauryl sulfate to cocamidopropyl betaine ranges from about 7.5:2.5 to about 8:2;

[0136] c) from about 0.40% to about 0.60%, by weight of the composition, of chloroxylenol; wherein the composition has a pH of from about 4 to about 5 and a viscosity of from about 3 Pa.s (3,000 cps) to about 25 Pa.s (25,000 cps).

[0137] Liquid hand washes may contain from about 0.025% to about 0.035%, by weight of the composition, of pyrrolidone carboxylic acid.

[0138] Preferably, the liquid hand detergent composition may comprise about 8% sodium lauryl sulfate, about 2% cocamidopropyl betaine, and 0.05% chloroxylenol.

[0139] More preferably, the liquid hand detergent composition may comprise about 8% sodium lauryl sulfate, about 2% cocamidopropyl betaine, about 0.03% pyrrolidone carboxylic acid, and 0.05% chloroxylenol.

[0140] method

[0141] The antibacterial cleaning compositions described herein may be intended for use as hand washes.

[0142] A method for washing hands and removing about 99% of germs is provided, the method comprising the following steps in order:

[0143] a) providing a container suitable for containing the antimicrobial cleaning composition described herein;

[0144] b) dispensing the antibacterial cleansing composition into the palm of a user;

[0145] c) optionally, lather by rubbing your palms together;

[0146] d) spreading the antibacterial cleansing composition on the palms, backs of hands, between fingers and / or under nails;

[0147] e) Wash your hands;

[0148] f) optionally drying; and

[0149] The antibacterial cleaning composition removes about 99% of bacteria.

[0150] The container may be a pump dispenser or a pump foam dispenser.

[0151] Users can wash their hands with hand sanitizer as follows:

[0152] 1) If using a liquid hand wash, wet your hands with clean running water (warm or cold), optionally turn off the tap, and apply soap. If using a foaming hand wash, rinsing your hands before applying soap is optional.

[0153] 2) If using a liquid hand soap, work up a lather by rubbing the palms and soap together. Work up a lather on the backs of hands, between fingers, and under nails. If using a foaming hand soap, spread the lather on the palms, backs of hands, between fingers, and under nails.

[0154] 3) It is recommended to scrub your hands for at least 20 seconds.

[0155] 4) Rinse hands well under clean running water.

[0156] 5) Dry your hands with a clean towel or let them air dry.

[0157] Washing your hands with the antimicrobial cleansing compositions described herein can help the skin maintain its natural barrier against bacteria.

[0158] Additionally, the antimicrobial cleaning compositions described herein can remove about 99% of bacteria.

[0159] The antibacterial cleaning composition described herein can be a hydrating hand wash. It can have a fresh clean scent, a nourishing aloe scent, an ocean breeze scent, a citrus scent, a lavender scent, and / or a coconut scent.

[0160] The antimicrobial cleansing compositions described herein can help the skin maintain its natural barrier against bacteria.

[0161] The antimicrobial cleansing compositions described herein can help maintain the skin's protective barrier.

[0162] The antimicrobial cleansing compositions described herein provide the skin with a first line of defense against bacteria.

[0163] The antibacterial cleaning composition can be easily dispersed by a pump and can be freely poured from any suitable container. In some examples, the antibacterial cleaning composition can be dispensed from a suitable container as a liquid or foam by squeezing the container.

[0164] Alternatively, the antimicrobial cleaning composition may be dispensed when the user actuates (eg, pushes down) the pump.

[0165] Alternatively, the antibacterial cleansing composition may be adapted for dispensing via a pump foamer, which is a non-aerosol means of dispensing the antibacterial cleansing composition as a foam that is typically mixed with air in a foaming chamber prior to expelling the composition.

[0166] use

[0167] All limitations and aspects as disclosed above with respect to the antibacterial cleaning composition may apply hereinafter.

[0168] An antimicrobial cleansing composition as described herein is provided for use as an antimicrobial medicament in the form of a product to be applied to human skin.

[0169] The antibacterial cleaning composition can be applied to the area in need of treatment. The area in need of treatment can be a keratinous tissue surface. Keratinous tissue refers to the keratin-containing layer that is the outermost protective covering of mammals, including but not limited to skin, hair, nails or epidermis.

[0170] The area to be treated may preferably be a human skin surface or a scalp surface.

[0171] Preferably, the antimicrobial cleansing compositions described herein may be provided for use in a method of inhibiting or killing the growth of bacteria on human skin.

[0172] More preferably, the antibacterial cleansing compositions described herein may be provided for use in a method of inhibiting or killing the growth of bacteria on human skin, wherein the bacteria is Staphylococcus aureus.

[0173] Alternatively, the antibacterial cleansing compositions described herein can be provided for use in a method of inhibiting or killing the growth of bacteria on human skin, wherein the bacteria are those listed in Table 1 as described in more detail below.

[0174] In fact, as shown in the Examples section, when the antimicrobial cleaning composition was evaluated under the Time Kill Test Method as disclosed herein, at least a 5-log or 99.999% reduction was observed for both the 30 and 60 second contact time intervals for all challenge microorganisms tested. The start and end counts for test culture viability were within ±0.5 Log 10 Inside.

[0175] The antimicrobial cleaning compositions described herein are used for non-therapeutic uses to provide a cleaning benefit.

[0176] The antimicrobial cleaning compositions described herein are useful for non-therapeutic use to improve hand hygiene.

[0177] Test Method

[0178] It should be understood that the test methods disclosed in the Test Methods section of this application should be used to determine the corresponding parameter values ​​of this invention as described and protected by applicant's invention.

[0179] Cone / Plate Viscosity Measurement Test Method

[0180] The viscosity of the examples was measured by a cone / plate controlled stress Brookfield rheometer R / S Plus from Brookfield Engineering Laboratories, Stoughton, MA. The cone (spindle C-75-1) used had a diameter of 75 mm and an angle of 1°. The viscosity was measured using a steady-state flow test at a temperature of 26.5° C. for 2 s -1The sample size was 2.5 ml and the total measurement reading time was 3 minutes.

[0181] Evaluation of antimicrobial activity using the time kill test method

[0182] scope

[0183] The Time Kill test method measures the in vitro reduction of the microbial population of a test organism after a specified contact time with a product formulation. Thus, when testing in vitro for antimicrobial test materials, this test method measures the change in aerobic and / or anaerobic microbial populations over a specific sampling time. The organisms used are standardized in terms of growth requirements and inoculum preparation and must be grown under the test conditions. The main purpose of this test method is to provide a set of standardized conditions and test organisms to facilitate comparative evaluation of antimicrobial substances that are miscible in aqueous systems.

[0184] Time-kill evaluations of product formulations (see Example 9 below) were conducted using a revised version of the following documents: ASTM Committee E35.15 Standard Test Method E2783-11 (2016) "Standard Test Method for Evaluating Antimicrobial Activity Against Water-Miscible Compounds Using a Time-Kill Procedure" and Food and Drug Administration Consumer Preservative Safety and Effectiveness; Topical Antimicrobial Drug Products for Over-the-Counter Human Use; Proposed Amendments to Tentative Final Monographs; Reopening of Administrative Records. June 2016.

[0185] The antimicrobial properties of the tested products were limited to the challenge microorganisms listed in Table 1 below after exposure within the specified sampling times of 30 seconds and 60 seconds after inoculation.

[0186] Table 1 Study parameters

[0187]

[0188]

[0189] equipment

[0190] Incubator capable of maintaining 35°C ± 1°C and 42°C ± 1°C

[0191] Incubator Thermometer, NIST Traceable

[0192] Vortex mixer

[0193] Calibrated, traceable minute / second timer

[0194] Refrigerator 2℃ to 8℃

[0195] Refrigerator Thermometer, NIST Traceable

[0196] Adjustable pipettes with capacities from 10μL to 100μL, 20μL to 200μL, and 100μL to 1000μL

[0197] Quebec Colony Counter

[0198] Traceable Thermometer / Clock / Humidity Monitor

[0199] Hand Crank Calculator

[0200] Electronic balance, sensitivity 0.1g

[0201] Anaerobic bacteria tank

[0202] Autoclave

[0203] Ultra-low temperature freezer capable of maintaining -70°C

[0204] Centrifuge

[0205] DensiCHEK Plus Device

[0206] Pipette

[0207] Material

[0208] Micropipette tips, 10μL to 100μL, 20μL to 200μL, and 100μL to 1000μL

[0209] Serological pipettes, 1mL, 2mL, 5mL, 10mL and 25mL

[0210] test tube

[0211] Disposable culture dish, 100mm×15mm

[0212] Flask or container

[0213] Appropriate size with closure for preparation of culture media and sterile deionized water

[0214] Fixed volume, 100mL and 1000mL

[0215] BD GasPak EZ Campy Container System for Microaerophilic Atmosphere Conditions (BD PN 260680)

[0216] Reagents

[0217] Neutralizer: Dilute to sub-inhibitory levels in Letheen Blank (LB) (MP088*)

[0218] Microbiological Content Test (MCT) Agar (MP107)

[0219] Tryptic Soy Agar (TSA) (MP064)

[0220] Phosphate buffered saline (PBS) (MP416)

[0221] Tryptic soy agar (SBA) containing 5% sheep blood is commercially available from BD, PN 221261

[0222] Campy Cefex Agar (CCA) (MP329)

[0223] Campylobacter CFP supplement - commercially available from Acumedia, PN 7981 - used as a supplement in CCA

[0224] Sterile lysed horse blood - commercially available from Hemostat, PN LHB 250 - used as a supplement in CCA

[0225] Sterile deionized water (DI water) (MP046)

[0226] Where appropriate, media / chemicals / reagents were purchased sterile or sterilized via autoclaving. *MP numbers refer to Q Laboratories Media Preparation numbers.

[0227] Method Description

[0228] Test microorganism preparation :

[0229] Seed batch culture maintenance techniques were used so that the viable microorganisms used for inoculation were no more than five generations removed from the original master seed batch.

[0230] For bacteria:

[0231] Cultures were propagated from Q Laboratories frozen stocks stored at -70°C on SBA at 35°C ± 1°C for 18 to 24 hours.

[0232] The culture was transferred to a TSA slants.

[0233] Each daily transfer was incubated at 35°C ± 1°C for 24 ± 2 hours.

[0234] Daily transfers of each culture were performed by aseptically transferring the growth to fresh agar.

[0235] For Campylobacter jejuni:

[0236] Cultures were propagated from Q Laboratories frozen stocks stored at -70°C on CCA at 42°C ± 1°C for 48 hours ± 2 hours under microaerobic atmosphere.

[0237] The culture was transferred onto CCA.

[0238] Each transfer was incubated at 42°C ± 1°C for 48 ± 2 hours.

[0239] Transfer of each culture was performed by aseptically transferring the growth to fresh agar.

[0240] Wash each agar slants with 3 mL of PBS to harvest the cells.

[0241] Use 1.0×10 9 CFU / mL. To verify the inoculum concentration, the DensiCHEK Plus instrument was used to measure the cell density to ensure that it was within

[0242] In the range of 3.5McFarland to 4.0McFarland.

[0243] After harvesting the cells, centrifuge any culture suspension <3.5 McFarland at 4,500 rpm for 15 min. Discard the supernatant, resuspend the pellet with 3 mL PBS, and verify the concentration.

[0244] Test procedures :

[0245] Use 0.1 mL aliquots of 10 9 A 10.0 mL portion of the product formulation was inoculated with a CFU / mL standardized challenge organism suspension.

[0246] The suspension was vortexed.

[0247] Maintain contact times of 30 sec ± 2 sec and 60 sec ± 3 sec.

[0248] At each contact time, 1.0 mL of the inoculum product was used to make ten-fold serial dilutions in LB neutralizer.

[0249] Dilutions were plated in duplicate via the pour plate technique.

[0250] C. jejuni was plated in duplicate using CCA and incubated at 42°C ± 1°C for 48 h ± 2 h under microaerobic atmospheric conditions.

[0251] All other organisms were plated in duplicate with MCT and incubated at 35°C ± 1°C for 48 h ± 2 h under aerobic atmospheric conditions.

[0252] Colonies were counted and recorded as CFU / plate to determine viable organisms.

[0253] Each sample, contact time, and organism was plated twice more for a total of three replicates.

[0254] Study Control

[0255] Testing culture viability :

[0256] To verify the viability of the inoculum, it was enumerated at the beginning and end of the test period.Ten-fold serial dilutions of each challenge organism suspension were prepared in duplicate by standard microbiological procedures to determine the inoculum population.

[0257] Colonies were enumerated and recorded as CFU / plate. The duplicate plates were averaged and multiplied by the dilution factor to calculate the microbial population of the suspension (CFU / mL).

[0258] The counts at the beginning and end of the test phase must be within ±0.5Log 10 to make the test effective.

[0259] Group control :

[0260] The inoculum population is determined by inoculating and analyzing a sterile water aliquot of the same volume and under the same conditions as the test product.

[0261] Inoculum suspensions were plated after the maximum contact time interval specified in the protocol.

[0262] Incubate the inoculum plates at the specified temperature and for a time appropriate to the test organism.

[0263] Neutralization Assessment :

[0264] The test methods outlined in this section ensure that no component of the neutralization process produces a detectable amount of inhibition against the target microorganisms that are intended to be recovered.

[0265] The test method outlined in this section also ensures the ability of the neutralization process to inactivate the antimicrobial properties of the tested product.

[0266] The neutralization process was performed according to ASTM method E1054-08 (reapproved in 2013), Standard Test Method for Evaluation of Antimicrobial Inactivators.

[0267] Neutralizer Effectiveness Test (NET) :

[0268] 1.0 mL of product was mixed with 9.0 mL of neutralizer, followed by the addition of 0.1 mL of test culture that had been serially diluted in PBS to yield final concentrations of 30 CFU / mL to 100 CFU / mL.

[0269] The suspension of cells, product, and neutralizer were mixed thoroughly and plated immediately, maintaining the maximum contact time for the timed kill assay and plating a second time.

[0270] The above process was repeated two more times for a total of three replicates.NET assessments were performed for each type of organism represented in the study (Gram-positive, Gram-negative, microaerophilic) using media, incubation times and temperatures appropriate to the organism of choice.

[0271] To demonstrate effective neutralization of the tested product, the average difference between the initial and final plate counts of NET must not exceed 0.2Log 10 .

[0272] Neutralizer Toxicity Test (NTT) :

[0273] 1.0 mL of PBS was mixed with 9.0 mL of neutralizer, followed by the addition of 0.1 mL of test culture that had been serially diluted in PBS to yield final concentrations of 30 CFU / mL to 100 CFU / mL.

[0274] The suspension of cells, PBS and neutralizer was mixed thoroughly and plated immediately, maintaining the maximum contact time for the time-kill assay and performing a second plating.

[0275] The above process was repeated two more times for a total of three replicates.NTT evaluations were performed for each type of organism represented in the study (Gram-positive, Gram-negative, microaerophilic) using the appropriate media, incubation time and temperature for the organism of choice.

[0276] To demonstrate that no component exerts an inhibitory effect during the neutralization process, the difference between the initial and final NTT plate counts must not exceed 0.2 Log 10 .

[0277] Test of Organism Viability (TOV) :

[0278] 10.0 mL of PBS was mixed followed by the addition of 0.1 mL of test culture that had been serially diluted in PBS to yield final concentrations of 30 CFU / mL to 100 CFU / mL.

[0279] The suspension, cells and PBS were mixed and plated immediately, maintaining the longest contact time for the time-kill assay and performing a second plating.

[0280] The above process was repeated two more times for a total of three replicates.TOV assessments were performed for each type of organism represented in the study (Gram-positive, Gram-negative, microaerophilic) using the appropriate media, incubation time and temperature for the organism of choice.

[0281] Culture medium quality control :

[0282] For MCT :

[0283] Duplicate plates were inoculated with 1 to 100 CFU of Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Aspergillus brasiliensis ATCC 16404, and Candida albicans ATCC 10231 and incubated at 30°C to 35°C for 3 days or less.

[0284] Duplicate plates of equivalent medium were inoculated with 1 to 100 CFU of Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Aspergillus brasiliensis ATCC 16404 and Candida albicans ATCC 10231 and incubated at 30°C to 35°C for 3 days or less.

[0285] One plate was incubated at 30°C to 35°C for 3 days or longer as a sterility control.

[0286] Comparable growth acceptance between media ranged from 5% to 200%. Sterile acceptance was no growth.

[0287] For CCA :

[0288] Duplicate plates were inoculated with 1 CFU to 100 CFU of Campylobacter jejuni ATCC 49943 and incubated at 42°C ± 1°C for 48 hours ± 2 hours under microaerobic atmospheric conditions.

[0289] Duplicate plates of equivalent medium were inoculated with 1 CFU to 100 CFU of Campylobacter jejuni ATCC 49943 and incubated at 42°C ± 1°C for 48 hours ± 2 hours under microaerobic atmospheric conditions.

[0290] One plate was incubated at 42°C ± 1°C for 48 hours ± 2 hours under microaerobic atmosphere.

[0291] Comparable growth acceptance between media was within 50% to 200%. Sterility acceptance was no growth.

[0292] Sterility assessment for PBS, LB, TSA slants and DI water :

[0293] One tube of each medium was incubated at 30°C to 35°C for 1 day or longer as a sterility control.

[0294] After incubation, streak onto normal growth agar. Incubate at 30°C to 35°C for 1 day or less.

[0295] Acceptance criterion was the absence of growth from the sterile control.

[0296] calculate

[0297] Neutralization Assessment :

[0298] If there was no difference between the mean initial and final counts (<0.2Log), the number of viable microorganisms from the final analysis of neutralizer efficacy and neutralizer toxicity was statistically compared to the final counts obtained in the test organism viability analysis using Student's t-test (p>0.05).

[0299] Neutralization was considered adequate if the organisms recovered from the neutralizer efficacy and neutralizer toxicity tests were not significantly different (p>0.05) from those recovered from the organism viability test.

[0300] A t-test assuming equal variances was used to determine the actual p-values ​​for the neutralization assessments.

[0301]

[0302] x1 and x2 = sample means

[0303] s 2 = Pooled sample variance

[0304] n1 and n2 = sample size

[0305] t = Student's t quantile, where n1+n2 are the degrees of freedom

[0306] The viable microbial populations of the population control and test replicates were measured for each microorganism under log transformation.

[0307] The log reduction is calculated as follows:

[0308] TP = Test Product

[0309] PC = population control

[0310] LR = Logarithmic Reduction

[0311] LR=Log 10 PC-Log 10 TP

[0312] The percentage reduction is calculated as follows:

[0313] PR = Percentage reduction

[0314]

[0315] Note: <1.0×10 1 Calculate as "10" to obtain percent and log reduction.

[0316] The difference between the initial and final time points for viability of the test organism was calculated as follows:

[0317] IC = Initial Count

[0318] FC = Final Count

[0319] TOV = Test Organism Viability Difference

[0320] TOV=Log 10 IC-Log 10 FC

[0321] Note: No statistically significant differences (<0.5Log 10 ).

[0322] Performance Standards

[0323] To prove that killing is acceptable, we can calculate the log of the surviving population. 10 The percentage reduction of the initial microbial population calculated from the baseline counts must be observed within the time interval for each challenge organism tested.

[0324] Acceptable Standards

[0325] The following study controls must be conducted according to detailed criteria for the data to be considered acceptable.

[0326] The start and end counts for test culture viability must be within ±0.5 Log 10 to make the test effective.

[0327] Neutralization was considered adequate if the organisms recovered from the neutralizer efficacy test were not significantly different (p>0.05) from those recovered from the organism viability test.

[0328] The culture medium must meet the specifications listed above for culture medium quality control.

[0329] Example

[0330] The following examples further describe and demonstrate embodiments within the scope of the present invention. These examples are provided for illustrative purposes only and should not be construed as limitations of the present invention, as many variations are possible without departing from the spirit and scope of the present invention. Applicable ingredients herein are identified by chemical name or CTFA name, unless otherwise defined below.

[0331] The following examples were prepared without fragrance:

[0332] Composition (weight %)

[0333]

[0334] Comparison of Examples 1 and 2 with the Comparative Examples shows that the weight ratio of sodium lauryl sulfate to the cosurfactant comprising cocamidopropyl betaine can directly affect the viscosity and achieve a target viscosity of 6,000 cps (6 Pa.s) acceptable to consumers. If the weight ratio of sodium lauryl sulfate to cosurfactant is too high (i.e., above 5:1), the viscosity of the antibacterial cleansing composition decreases, which makes it too dilute to be acceptable to consumers and fails to deliver the antibacterial active chloroxylenol to the skin.

[0335] Furthermore, the above results show that when the weight ratio of sodium lauryl sulfate to the cosurfactant comprising cocamidopropyl betaine is too low (i.e., below 3:1), the viscosity is 400 cps to 900 cps, which is unacceptable to consumers and fails to deliver the antimicrobial active chloroxylenol to the skin.

[0336] When it is optionally desired to add fragrance to the antimicrobial cleansing composition, the same results were obtained by increasing the fragrance level to 0.02%, 0.40% and 0.60%.

[0337] The following examples were prepared using different levels of fragrance:

[0338] Composition (weight %)

[0339]

[0340]

[0341]

[0342] Antimicrobial Cleansing Composition Example 9 has been evaluated for in vitro time kill efficacy using the Time Kill Test Method.

[0343] Composition (weight %)

[0344] Example 9 <![CDATA[Sodium lauryl sulfate (SLS) 1 > 8.00 <![CDATA[Cocamidopropyl betaine (CAPB) 2 > 2.00 Sodium Benzoate 0.25 Disodium EDTA 0.10 <![CDATA[Chloroxylenol 3 > 0.50 Citric Acid 1.30 Benzyl alcohol 0.30 <![CDATA[Methylchloroisothiazolinone, Methylisothiazolinone 4 > 0.03 <![CDATA[Pyrrolidone carboxylic acid 5 > 0.03 Salicylic acid 0.20 Fragrance 0.40 pH 3.3 water Moderate SLS / CAPB Ratio 8:2 Product viscosity (cps) 6,000

[0345] 1. Purchased from Procter & Gamble

[0346] 2. Purchased from

[0347] 3. Purchased from Thermo Scientific

[0348] 4. Kathon TM CG, purchased from Rohm&Hass

[0349] 5. A100, purchased from Co., Inc.

[0350] Table 2 In vitro timed killing efficacy of antibacterial cleaning composition Example 9

[0351]

[0352]

[0353] Table 3 Timed Killing Evaluation of Example 9 at 30 Seconds Contact Time

[0354]

[0355]

[0356]

[0357] Example 9: Similar results were obtained in the timed kill evaluation at 60 seconds contact time.

[0358] Table 4 Example 9 Neutralizer Effect and Toxicity Test Results (CFU / mL)

[0359]

[0360] 1 No statistically significant differences (<0.2Log 10 ).

[0361] Table 5 Example 9 Neutralizer Effect and Toxicity Test

[0362]

[0363]

[0364] 1*T-test comparing NET and NTT to TOV showed no statistical significance (p>0.05).

[0365] The above results show consistent antimicrobial efficacy, delivering >5 log reductions against all challenged microorganisms at 30 and 60 seconds of contact with the composition of Example 9 containing 0.5 wt % chloroxylenol, which meets the minimum >3 log reduction required in the FDA monograph.

[0366] In other words, based on these results, for Example 9, at least a 5 log or 99.999% reduction was observed for both the 30 and 60 second contact time intervals for all challenge microorganisms tested. The start and end counts for test culture viability were within ±0.5 Log 10 Inside.

[0367] The organisms recovered from the neutralizer efficacy and neutralizer toxicity tests were not significantly different from those recovered from the organism viability test (p>0.05). All media controls passed specifications.

[0368] Additional embodiments / combinations

[0369] A. An antibacterial cleaning composition, comprising:

[0370] a) 0.01% to 1% by weight of the composition of chloroxylenol;

[0371] b) 5% to 15% by weight of the composition of sodium lauryl sulfate;

[0372] c) 1% to 5% by weight of the composition of a co-surfactant, wherein the co-surfactant is selected from zwitterionic surfactants, amphoteric surfactants, nonionic surfactants, or combinations thereof;

[0373] d) wherein the weight ratio of sodium lauryl sulfate to the co-surfactant is in the range of 3:1 to 5:1, preferably 7.5:2.5 to 8:2; and

[0374] e) wherein the composition has a pH of 2 to 5.

[0375] B. The antibacterial cleaning composition of paragraph A, comprising 0.10% to 0.75% chloroxylenol by weight of the composition; preferably 0.40% to 0.60% chloroxylenol by weight of the composition.

[0376] C. An antibacterial cleaning composition according to any of the preceding paragraphs, comprising from about 0.005% to about 5% pyrrolidone carboxylic acid by weight of the composition; preferably 0.02% to 1% pyrrolidone carboxylic acid by weight of the composition; more preferably 0.025% to 0.15% pyrrolidone carboxylic acid by weight of the composition; even more preferably from about 0.025% to about 0.05% pyrrolidone carboxylic acid by weight of the composition; most preferably from about 0.025% to about 0.035% pyrrolidone carboxylic acid by weight of the composition.

[0377] D. An antibacterial cleaning composition according to any of the preceding paragraphs, wherein the composition comprises 7% to 9% sodium lauryl sulfate by weight of the composition; preferably wherein the composition comprises 7.5% to 8% sodium lauryl sulfate by weight of the composition.

[0378] E. The antibacterial cleaning composition of any of the preceding paragraphs, wherein the composition comprises 8% to 10% sodium lauryl sulfate, and wherein the antibacterial cleaning composition is a liquid hand detergent composition.

[0379] F. An antibacterial cleaning composition according to any of the preceding paragraphs, wherein the composition comprises from 1.4% to 3.0% by weight of the composition of a co-surfactant; preferably from 2.0% to 2.5% by weight of the composition of a co-surfactant.

[0380] G. An antimicrobial cleaning composition according to any of the preceding paragraphs, wherein the composition comprises 7.5% to 8%, by weight of the composition, of sodium lauryl sulfate and 2.0% to 2.5%, by weight of the composition, of a co-surfactant.

[0381] H. An antibacterial cleaning composition according to any of the preceding paragraphs, wherein the co-surfactant comprises cocamidopropyl betaine.

[0382] I. The antibacterial cleaning composition according to paragraph H, wherein the antibacterial cleaning composition has a weight ratio of sodium lauryl sulfate to cocamidopropyl betaine in the range of 3:1 to 5:1, preferably 7.5:2.5 to 8:2; more preferably 8:2.

[0383] J. An antibacterial cleaning composition according to any of the preceding paragraphs, wherein the pH is from 3 to 3.5.

[0384] K. An antibacterial cleaning composition according to any of the preceding paragraphs, wherein the antibacterial cleaning composition has a viscosity of 2.75 Pa.s (2,750 cps) to 30 Pa.s (30,000 cps); preferably 3 Pa.s (3,000 cps) to 25 Pa.s (25,000 cps); more preferably 3.4 Pa.s (3,400 cps) to 24.25 Pa.s (24,250 cps), as measured according to the Cone / Plate Viscosity Measurement Test Method disclosed herein.

[0385] L. A method of washing hands and removing 99% of germs, the method comprising the following steps in order:

[0386] a) providing a container suitable for containing an antimicrobial cleaning composition according to any of the preceding paragraphs;

[0387] b) dispensing the antibacterial cleansing composition into the palm of a user;

[0388] c) optionally, lather by rubbing your palms together;

[0389] d) spreading the antibacterial cleaning composition on the palm, back of the hand, between the fingers and / or

[0390] or under the nails;

[0391] e) Wash your hands;

[0392] f) optionally drying;

[0393] g) wherein the antibacterial cleaning composition removes 99% of bacteria.

[0394] M. The method according to paragraph L, wherein the container is a pump dispenser or a pump foam dispenser.

[0395] N. A liquid hand washing composition, comprising:

[0396] a) 7% to 9%, preferably 7.5% to 8%, by weight of the composition, of sodium lauryl sulfate;

[0397] b) 1.75% to 3%, preferably 2% to 2.5% by weight of the composition of cocamidopropyl betaine;

[0398] c) wherein the weight ratio of sodium lauryl sulfate to cocamidopropyl betaine is in the range of 7.5:2.5 to 8:2;

[0399] d) 0.40% to 0.60%, by weight of the composition, of chloroxylenol; and

[0400] wherein the composition has a pH of 4 to 5 and a viscosity of 5 Pa.s (5000 cps) to 10 Pa.s (10,000 cps) as measured according to the Cone / Plate Viscosity Measurement Test Method as disclosed herein.

[0401] O. A liquid handwash composition according to any of the preceding paragraphs N, comprising 0.025% to 0.035% pyrrolidone carboxylic acid by weight of the composition.

[0402] P. A liquid hand wash composition according to any one of the preceding paragraphs N to O, wherein the liquid hand wash composition comprises 8% sodium lauryl sulfate, 2% cocamidopropyl betaine and 0.05% chloroxylenol; preferably wherein the liquid hand wash composition comprises 8% sodium lauryl sulfate, 2% cocamidopropyl betaine, 0.03% pyrrolidone carboxylic acid and 0.05% chloroxylenol.

[0403] Q. An antibacterial cleansing composition according to any one of the preceding paragraphs A to K, which is used as an antibacterial drug in the form of a product to be applied to human skin.

[0404] R. An antibacterial cleaning composition according to any one of the preceding paragraphs A to K, wherein the antibacterial cleaning composition is used in a method for inhibiting or killing the growth of bacteria on human skin; preferably used in a method for inhibiting or killing the growth of bacteria on human skin, wherein the bacteria is Staphylococcus aureus.

[0405] S. The antibacterial cleaning composition according to any of the preceding paragraphs A to K, for use in a method of inhibiting or killing the growth of bacteria on human skin, wherein the bacteria are listed in Table 1 as described in detail below.

[0406] T. Non-therapeutic use of an antimicrobial cleaning composition according to any of the preceding paragraphs A to K to provide a cleaning benefit.

[0407] U. Non-therapeutic use of the antibacterial cleaning composition according to any of the preceding paragraphs A to K for improving hand hygiene.

[0408] The dimensions and values ​​disclosed herein should not be understood as being strictly limited to the exact numerical values ​​cited. Instead, unless otherwise indicated, each such dimension is intended to represent the stated value and a functionally equivalent range around that value. For example, a dimension disclosed as "40 mm" is intended to mean "about 40 mm".

[0409] Unless expressly excluded or otherwise limited, each document cited herein, including any cross-referenced or related patent or application, is incorporated herein by reference in its entirety. Citation of any document is not an admission that it is prior art to any of the present invention disclosed or claimed herein, or that it, by itself or in combination with any one or more references, proposes, suggests, or discloses any such invention. In addition, to the extent that any meaning or definition of a term in this invention conflicts with any meaning or definition of the same term in a document incorporated by reference, the meaning or definition assigned to that term in this invention shall govern.

[0410] Although specific embodiments of the present invention have been illustrated and described, it will be apparent to those skilled in the art that various other changes and modifications may be made without departing from the spirit and scope of the present invention. Therefore, it is intended that all such changes and modifications within the scope of the present invention be covered in the appended claims.

Claims

1. An antibacterial cleaning composition, comprising: a) 0.01% to 1% by weight of the composition of chloroxylenol; b) 5% to 15% by weight of the composition of sodium lauryl sulfate; c) 1% to 5% by weight of the composition of a co-surfactant, wherein the co-surfactant is selected from zwitterionic surfactants, amphoteric surfactants, nonionic surfactants, or combinations thereof; d) wherein the weight ratio of the sodium lauryl sulfate to the co-surfactant is in the range of 3:1 to 5:1; and wherein the composition has a pH of 2 to 5.

2. An antibacterial cleaning composition according to claim 1, wherein the composition comprises 0.10% to 0.75% chloroxylenol by weight of the composition, preferably 0.40% to 0.60% chloroxylenol by weight of the composition.

3. An antibacterial cleaning composition according to any one of the preceding claims, wherein the composition comprises 0.005% to 5% pyrrolidone carboxylic acid by weight of the composition, preferably 0.02% to 1% pyrrolidone carboxylic acid by weight of the composition; more preferably 0.025% to 0.15% pyrrolidone carboxylic acid by weight of the composition; even more preferably 0.025% to 0.05% pyrrolidone carboxylic acid by weight of the composition; most preferably 0.025% to 0.035% pyrrolidone carboxylic acid by weight of the composition.

4. An antibacterial cleaning composition according to any one of the preceding claims, wherein the composition comprises 7% to 9% sodium lauryl sulfate, and wherein the antibacterial cleaning composition is a liquid hand detergent composition; preferably wherein the composition comprises 7.5% to 8% sodium lauryl sulfate by weight of the composition.

5. An antibacterial cleaning composition according to any preceding claim, wherein the composition comprises from 1.4% to 3.0% by weight of the composition of a co-surfactant; preferably from 2.0% to 2.5% by weight of the composition of a co-surfactant.

6. The antimicrobial cleaning composition of claim 5, wherein the auxiliary surfactant comprises cocamidopropyl betaine.

7. The antibacterial cleaning composition according to claim 6, wherein the antibacterial cleaning composition has a weight ratio of sodium lauryl sulfate to cocamidopropyl betaine in the range of 3:1 to 5:1, preferably 7.5:2.5 to 8:2; more preferably 8:

2.

8. An antimicrobial cleaning composition according to any one of the preceding claims, wherein the pH is from 3 to 3.

5.

9. An antibacterial cleaning composition according to any of the preceding claims, wherein the composition has a viscosity of 2.75 Pa.s (2,750 cps) to 30 Pa.s (30,000 cps); preferably 3 Pa.s (3,000 cps) to 25 Pa.s (25,000 cps); more preferably 3.4 Pa.s (3,400 cps) to 24.25 Pa.s (24,250 cps), most preferably 5.0 Pa.s (5,000 cps) to 10.0 Pa.s (10,000 cps), as measured according to the Cone / Plate Viscosity Measurement Test Method disclosed herein.

10. A method of washing hands and removing 99% of bacteria, the method being in the order The following steps are involved: a) providing a container suitable for containing an antimicrobial cleaning composition according to any one of the preceding claims; b) dispensing the antibacterial cleansing composition into the palm of a user; c) optionally, lather by rubbing your palms together; d) spreading the antibacterial cleaning composition on the palm, back of the hand, between the fingers and / or under the nails; e) Wash your hands; f) optionally drying; g) wherein the antibacterial cleaning composition removes 99% of bacteria.

11. The method of claim 10, wherein the container is a pump dispenser or a pump foam dispenser.

12. A liquid hand washing composition, comprising: a) 7% to 9%, preferably 7.5% to 8%, by weight of the composition, of sodium lauryl sulfate; b) 1.75% to 3%, preferably 2% to 2.5% by weight of the composition of cocamidopropyl betaine; c) wherein the weight ratio of sodium lauryl sulfate to cocamidopropyl betaine is in the range of 7.5:2.5 to 8:2; d) 0.40% to 0.60%, by weight of the composition, of chloroxylenol; and wherein the composition has a pH of 4 to 5 and a viscosity of 5.0 Pa.s (5000 cps) to 10 Pa.s (10,000 cps) as measured according to the Cone / Plate Viscosity Measurement Test Method as disclosed herein.

13. The liquid hand washing composition according to claim 12, wherein the liquid hand washing composition comprises 8% sodium lauryl sulfate, 2% cocamidopropyl betaine and 0.05% chloroxylenol; preferably wherein the liquid hand washing composition comprises 8% sodium lauryl sulfate, 2% cocamidopropyl betaine, 0.03% pyrrolidone carboxylic acid and 0.05% chloroxylenol.

14. An antibacterial cleansing composition according to any one of the preceding claims 1 to 9 for use as an antibacterial medicament in the form of a product to be applied to human skin.

15. An antibacterial cleaning composition according to any one of the preceding claims 1 to 9 for use in a method of inhibiting or killing the growth of bacteria on human skin; preferably for use in a method of inhibiting or killing the growth of bacteria on human skin, wherein the bacteria is Staphylococcus aureus.

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