Method for repairing citrus liberobacter asiaticum tree body

Abstract

The invention discloses a method for repairing citrus liberobacter asiaticum trees, and relates to the technical field of plant cultivation. A pesticide application technology combining trunk infusion and leaf surface spraying is adopted for a tree body suffering from the citrus liberobacter asiaticum, the first time of trunk infusion is started from the middle ten days to the last ten days of May, and then the interval time of each time of infusion does not exceed one month; leaf surface spraying is combined with weather conditions, sterilization and insecticide are sprayed on leaf surfaces about 15 days after each time of trunk infusion, and spraying is carried out four times in total. According to the method, appropriate agents are selected for mutual matching, meanwhile, the reasonable time and frequency of trunk infusion and leaf surface spraying are set, and the two agents are used in a crossed mode, so that the repairing effect is stable; the method is simple in scheme, raw materials are easy to obtain, and the method has the characteristics of high efficiency, low toxicity, safety and economy, is convenient for large-area popularization, and can better repair citrus liberobacter asiaticum trees.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, and in particular to a method for repairing citrus Huanglongbing trees. Background Art

[0002] Citrus, as one of the main cash crops in my country, plays a vital role in promoting the development of agricultural economy. However, the outbreak of citrus Huanglongbing during citrus cultivation in recent years has dealt a heavy blow to my country's citrus industry. Citrus Huanglongbing is a bacterial citrus disease caused by Bacillus asiatica. The pathogen can be transmitted through a variety of pathways, with diseased plants and vector insects as the main transmission vectors. Citrus psyllids are the main insect-source vectors of citrus Huanglongbing. After sucking the sap of diseased trees, psyllids will carry the pathogen for life and transmit the pathogen to healthy plants when sucking healthy plants, causing the spread of citrus Huanglongbing. Transmission by agricultural tools and wind and rain are also the main transmission pathways of the disease. As the disease progresses, the photosynthesis capacity of citrus trees weakens, leading to the decline of tree vigor and ultimately a sharp drop in citrus production. The fruits of diseased trees are often of poor quality, and the taste and appearance do not meet market requirements, seriously affecting the economic value of citrus.

[0003] At present, the prevention and control of Huanglongbing mainly involves killing psyllids, cutting down diseased trees, and planting healthy seedlings, which are commonly known as the "three axes" for preventing and controlling Huanglongbing. However, there is currently no dedicated treatment agent for trees that have been infected with Huanglongbing, how to use drugs to prevent and control Huanglongbing pathogens, improve tree resistance to diseases, and alleviate the impact of Huanglongbing on trees and fruits. The present invention aims to implement trunk infusion combined with foliar spraying technology on diseased trees that have been infected with Huanglongbing, forming a method for repairing citrus Huanglongbing trees. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention proposes a method for repairing citrus Huanglongbing trees to solve the problems raised by the above-mentioned background technology.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0007] (1) Trunk infusion: Drill a hole 20 to 50 cm above the ground in the trunk of the tree and inject liquid A. Use two needles each time to inject liquid A. The two needles are located on opposite sides of the trunk and are injected at the same level.

[0008] The medicinal solution A comprises the following raw materials in parts by weight: 30-45 parts of 500-fold liquid of 99.6% ampicillin, 15-25 parts of 500-fold liquid of 20% amoxicillin, 15-22 parts of 1000-fold liquid of 0.1% triacontanol, 10-18 parts of 600-fold liquid of 23% copper rosinate, 7-14 parts of 500-fold liquid of 80% mancozeb, 5-10 parts of 1000-fold liquid of 19% calcium magnesium trace water-soluble fertilizer, and 100-200 parts of water;

[0009] (2) Foliar spray: 12 to 18 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4;

[0010] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 22-35 parts of 200-fold solution of 1.26% cephalosporin acetate, 20-25 parts of 300-fold solution of 20% thiophanate-methyl, 8-20 parts of 2000-fold solution of 20% dinotefuran, and 50-150 parts of water;

[0011] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 8 to 15 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 7 to 12 parts of 500 times solution of 30% nonyl copper, 3 to 9 parts of 2000 times solution of 25% thiamethoxam, and 30 to 70 parts of water;

[0012] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 16-30 parts of 600-fold solution of 47% Spring Thunder·Royal Copper, 12-26 parts of 300-fold solution of 20% Thiazole Zinc, 5-13 parts of 2000-fold solution of 22% Sulfoxaflor, and 50-100 parts of water;

[0013] The B4 is the fourth foliar spray solution, which includes the following raw materials in parts by weight: 10 to 17 parts of 750 times liquid of 80% ethoxypropynyl, 8 to 15 parts of 800 times liquid of 3% sinomenine, 7 to 12 parts of 3000 times liquid of 70% imidacloprid, and 60 to 90 parts of water.

[0014] Preferably, the calcium and magnesium trace water-soluble fertilizer is purchased from Yantai Hongyuan Biofertilizer Co., Ltd., the medium and trace element water-soluble fertilizer-calcium and magnesium No. 1, the ratio of calcium and magnesium is Ca≥11% and Mg≥3% respectively.

[0015] Preferably, the first main infusion in step (1) starts in mid-May or late May.

[0016] Preferably, in step (1), one of the two needles reaches deep into the xylem of the tree, and the other needle penetrates deeply into the phloem.

[0017] Preferably, the dosage of the medicinal solution A in step (1) is 150-300 mL / plant, and the infusion is performed 4 times in total, with an interval of 20-30 days between each infusion.

[0018] Preferably, the medicinal solution A in step (1) comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water.

[0019] Preferably, the spraying amount of the liquid B in step (2) is 10-25 mL / plant, and the leaves are sprayed 4 times in total.

[0020] Preferably, B1 in step (2) is the first foliar spray solution, comprising the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cefotaxime acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water.

[0021] Preferably, B2 in step (2) is the second foliar spray solution, comprising the following raw materials in parts by weight: 12 parts of 1000 times liquid of 50% chlorobromoisocyanuric acid, 10 parts of 500 times liquid of 30% nonyl copper, 6 parts of 2000 times liquid of 25% thiamethoxam, and 60 parts of water.

[0022] Preferably, B3 in step (2) is the third foliar spray solution, comprising the following raw materials in parts by weight: 28 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 19 parts of 300 times liquid of 20% thiabendazole, 10 parts of 2000 times liquid of 22% sulfoxaflor, and 70 parts of water.

[0023] Preferably, B4 in step (2) is the fourth foliar spray solution, comprising the following raw materials in parts by weight: 12 parts of 750 times liquid of 80% ethionine, 13 parts of 800 times liquid of 3% sinomenine, 10 parts of 3000 times liquid of 70% imidacloprid, and 80 parts of water.

[0024] Preferably, the method for repairing citrus Huanglongbing disease comprises the following steps:

[0025] S1. Mix liquid A with purified water to prepare 200 ml of aqueous solution; inject the aqueous solution into the citrus tree through the drill hole;

[0026] S2. Mix different fungicides and insecticides with purified water to prepare 15 kg aqueous solution; spray the solution on the leaves of citrus trees using a sprayer.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] (1) Trunk injection technology can transport the liquid medicine to the entire tree through the xylem and phloem to the site where the pathogen is parasitic, greatly improving the effectiveness of the liquid medicine and reducing the amount of medicine required. The liquid medicine is sealed in the tree, so less medicine leaks into the external environment, reducing the chance of contact with the human body. It is safe and low-pollution, and can more efficiently repair Huanglongbing trees.

[0029] (2) The invention adopts a pesticide application technology that combines trunk infusion with foliar spraying. The invention selects appropriate agents to match each other, and at the same time sets a reasonable time and frequency for trunk infusion and foliar spraying, and the two are used interchangeably to stabilize the repair effect. The invention has a simple scheme, and the raw materials are easily available. It has the characteristics of high efficiency, low toxicity, safety, and economy, and is easy to promote on a large scale, and can better repair citrus Huanglongbing trees. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 Conduct testing of citrus trees infected with Huanglongbing pathogen;

[0031] Figure 2 Drilling of citrus trees affected by Huanglongbing;

[0032] Figure 3 Perform trunk infusions on citrus trees affected by Huanglongbing;

[0033] Figure 4 Apply foliar spray to citrus trees affected by Huanglongbing. DETAILED DESCRIPTION

[0034] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0035] Example 1

[0036] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0037] (1) Trunk infusion: In mid-May, a hole was drilled 20 cm above the ground in the trunk of the tree to infuse Solution A. Two needles were used for each infusion of Solution A. One needle reached deep into the xylem of the tree, and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk, and Solution A was injected at the same level. The dosage of Solution A was 150 mL / plant, and the infusion was performed 4 times in total, with an interval of 20 days between each infusion.

[0038] The drug solution A comprises the following raw materials in parts by weight: 30 parts of 500 times liquid of 99.6% ampicillin, 15 parts of 500 times liquid of 20% amoxicillin, 15 parts of 1000 times liquid of 0.1% triacontanol, 10 parts of 600 times liquid of 23% copper rosinate, 7 parts of 500 times liquid of 80% mancozeb, 5 parts of 19% calcium magnesium trace water-soluble fertilizer, 1000 times liquid, and 100 parts of water;

[0039] (2) Foliar spray: 12 days after each trunk infusion, spray the leaves with liquid B, the spraying amount of liquid B is 10 mL / plant, and the leaves are sprayed 4 times in total. The liquid B is divided into B1, B2, B3 and B4;

[0040] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 22 parts of 200 times solution of 1.26% cephalosporin acetate, 20 parts of 300 times solution of 20% thiophanate-methyl, 8 parts of 2000 times solution of 20% dinotefuran, and 50 parts of water;

[0041] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 8 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 7 parts of 500 times solution of 30% nonyl copper, 3 parts of 2000 times solution of 25% thiamethoxam, and 30 parts of water;

[0042] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 16 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 12 parts of 300 times liquid of 20% Thiazole Zinc, 5 parts of 2000 times liquid of 22% Sulfoxaflor, and 50 parts of water;

[0043] The B4 is the fourth foliar spray solution, which includes the following raw materials in parts by weight: 10 parts of 750 times liquid of 80% ethoxypropynyl, 8 parts of 800 times liquid of 3% sinomenine, 7 parts of 3000 times liquid of 70% imidacloprid, and 60 parts of water.

[0044] Example 2

[0045] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0046] (1) Trunk infusion: In late May, a hole was drilled 50 cm above the ground in the trunk of the tree and liquid A was infused. Two needles were used for each infusion of liquid A. One needle reached deep into the xylem of the tree and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk and liquid A was injected at the same level. The dosage of liquid A was 300 mL / plant, and the infusion was performed 4 times in total, with an interval of 30 days between each infusion.

[0047] The drug solution A comprises the following raw materials in parts by weight: 45 parts of 500 times liquid of 99.6% ampicillin, 25 parts of 500 times liquid of 20% amoxicillin, 22 parts of 1000 times liquid of 0.1% triacontanol, 18 parts of 600 times liquid of 23% copper rosinate, 14 parts of 500 times liquid of 80% mancozeb, 10 parts of 19% calcium magnesium trace water-soluble fertilizer, 1000 times liquid, and 200 parts of water;

[0048] (2) Foliar spray: 18 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; the spraying amount of liquid B is 25 mL / plant, and the leaves are sprayed 4 times in total;

[0049] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 35 parts of 200 times solution of 1.26% cefotaxime acetate, 25 parts of 300 times solution of 20% thiophanate-methyl, 20 parts of 2000 times solution of 20% dinotefuran, and 150 parts of water;

[0050] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 15 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 12 parts of 500 times solution of 30% nonyl copper, 9 parts of 2000 times solution of 25% thiamethoxam, and 70 parts of water;

[0051] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 26 parts of 300 times liquid of 20% Thiazole Zinc, 13 parts of 2000 times liquid of 22% Sulfoxaflor, and 100 parts of water;

[0052] The B4 is the fourth foliar spray solution, which includes the following raw materials in parts by weight: 17 parts of 750 times liquid of 80% ethoxypropynyl, 15 parts of 800 times liquid of 3% sinomenine, 12 parts of 3000 times liquid of 70% imidacloprid, and 90 parts of water.

[0053] Example 3

[0054] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0055] (1) Trunk infusion: In mid-May, a hole was drilled 30 cm above the ground in the trunk of the tree to infuse Solution A. Two needles were used each time for infusion of Solution A. One needle reached deep into the xylem of the tree, and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk, and Solution A was injected at the same level. The dosage of Solution A was 250 mL / plant, and the infusion was performed 4 times in total, with an interval of 25 days between each infusion.

[0056] The drug solution A comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water;

[0057] (2) Foliar spray: 15 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; the spraying amount of liquid B is 20 mL / plant, and the leaves are sprayed 4 times in total;

[0058] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cephalosporin acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water;

[0059] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 10 parts of 500 times solution of 30% nonyl copper, 6 parts of 2000 times solution of 25% thiamethoxam, and 60 parts of water;

[0060] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 28 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 19 parts of 300 times liquid of 20% Thiazole Zinc, 10 parts of 2000 times liquid of 22% Sulfoxaflor, and 70 parts of water;

[0061] The B4 is the fourth foliar spray solution, which includes the following raw materials in weight: 12 parts of 750 times 80% ethoxylated solution, 13 parts of 800 times 3% sinomenine solution, 10 parts of 3000 times 70% imidacloprid solution, and 80 parts of water.

[0062] Comparative Example 1

[0063] The difference between this comparative example and Example 3 is that the main infusion is injected with only one needle, and the other components and steps remain unchanged.

[0064] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0065] (1) Trunk infusion: In mid-May, drill a hole 30 cm above the ground in the trunk of the tree and infuse liquid A. Use one needle each time to infuse liquid A, and the needle should reach the xylem of the tree. The dosage of liquid A is 250 mL / tree, and the infusion is performed 4 times in total, with an interval of 25 days between each infusion.

[0066] The drug solution A comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water;

[0067] (2) Foliar spray: 15 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; the spraying amount of liquid B is 20 mL / plant, and the leaves are sprayed 4 times in total

[0068] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cephalosporin acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water;

[0069] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 10 parts of 500 times solution of 30% nonyl copper, 6 parts of 2000 times solution of 25% thiamethoxam, and 60 parts of water;

[0070] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 28 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 19 parts of 300 times liquid of 20% Thiazole Zinc, 10 parts of 2000 times liquid of 22% Sulfoxaflor, and 70 parts of water;

[0071] The B4 is the fourth foliar spray solution, which includes the following raw materials in weight: 12 parts of 750 times 80% ethoxylated solution, 13 parts of 800 times 3% sinomenine solution, 10 parts of 3000 times 70% imidacloprid solution, and 80 parts of water.

[0072] Comparative Example 2

[0073] The difference between this comparative example and Example 3 is that B1 was used for the 4 foliar sprays, and the other ingredients and steps remained unchanged.

[0074] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0075] (1) Trunk infusion: In mid-May, a hole was drilled 30 cm above the ground in the trunk of the tree to infuse Solution A. Two needles were used each time for infusion of Solution A. One needle reached deep into the xylem of the tree, and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk, and Solution A was injected at the same level. The dosage of Solution A was 250 mL / plant, and the infusion was performed 4 times in total, with an interval of 25 days between each infusion.

[0076] The drug solution A comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water;

[0077] (2) Foliar spray: 15 days after each trunk infusion, spray the leaves with liquid B1 at a spray rate of 20 mL / plant, for a total of 4 foliar sprays;

[0078] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cephalosporin acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water;

[0079] Comparative Example 3

[0080] The difference between this comparative example and Example 3 is that the method for repairing citrus Huanglongbing trees only uses the trunk infusion method, and the other components and steps remain unchanged.

[0081] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0082] (1) Trunk infusion: In mid-May, a hole was drilled 30 cm above the ground in the trunk of the tree to infuse Solution A. Two needles were used each time for infusion of Solution A. One needle reached deep into the xylem of the tree, and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk, and Solution A was injected at the same level. The dosage of Solution A was 250 mL / plant, and the infusion was performed 4 times in total, with an interval of 25 days between each infusion.

[0083] The drug solution A comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water;

[0084] Comparative Example 4

[0085] The difference between this comparative example and Example 3 is that the method for repairing citrus Huanglongbing trees only uses the foliar spray method, and the other components and steps remain unchanged.

[0086] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0087] (1) Foliar spray: In mid-May, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; the spraying amount of liquid B is 20 mL / plant, and the leaves are sprayed 4 times in total, with an interval of 25 days between each spraying;

[0088] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cephalosporin acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water;

[0089] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 10 parts of 500 times solution of 30% nonyl copper, 6 parts of 2000 times solution of 25% thiamethoxam, and 60 parts of water;

[0090] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 28 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 19 parts of 300 times liquid of 20% Thiazole Zinc, 10 parts of 2000 times liquid of 22% Sulfoxaflor, and 70 parts of water;

[0091] The B4 is the fourth foliar spray solution, which includes the following raw materials in weight: 12 parts of 750 times 80% ethoxylated solution, 13 parts of 800 times 3% sinomenine solution, 10 parts of 3000 times 70% imidacloprid solution, and 80 parts of water.

[0092] Comparative Example 5

[0093] The difference between this comparative example and Example 3 is that: the raw material components of the medicinal solution A do not contain 20% amoxicillin 500 times solution, and the other components and steps remain unchanged.

[0094] A method for repairing citrus Huanglongbing trees, comprising the following steps:

[0095] (1) Trunk infusion: In mid-May, a hole was drilled 30 cm above the ground in the trunk of the tree to infuse Solution A. Two needles were used each time for infusion of Solution A. One needle reached deep into the xylem of the tree, and the other needle penetrated deeply into the phloem. The two needles were located on opposite sides of the trunk, and Solution A was injected at the same level. The dosage of Solution A was 250 mL / plant, and the infusion was performed 4 times in total, with an interval of 25 days between each infusion.

[0096] The drug solution A comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water;

[0097] (2) Foliar spray: 15 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; the spraying amount of liquid B is 20 mL / plant, and the leaves are sprayed 4 times in total;

[0098] B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cephalosporin acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water;

[0099] B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 10 parts of 500 times solution of 30% nonyl copper, 6 parts of 2000 times solution of 25% thiamethoxam, and 60 parts of water;

[0100] B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 28 parts of 600 times liquid of 47% Spring Thunder·Royal Copper, 19 parts of 300 times liquid of 20% Thiazole Zinc, 10 parts of 2000 times liquid of 22% Sulfoxaflor, and 70 parts of water;

[0101] The B4 is the fourth foliar spray solution, which includes the following raw materials in weight: 12 parts of 750 times 80% ethoxylated solution, 13 parts of 800 times 3% sinomenine solution, 10 parts of 3000 times 70% imidacloprid solution, and 80 parts of water.

[0102] Test example

[0103] (1) Experimental location: Citrus plantation in Shaziwo Village, Yongxing County, Hunan Province; selected plots with convenient drainage and irrigation, good light conditions, and soil conditions that meet the citrus growth conditions as the experimental area;

[0104] (2) Test subjects: The citrus Huanglongbing trees treated with Examples 1 to 3 and Comparative Examples 1 to 5 were used as the experimental group, the citrus Huanglongbing trees treated with clean water were used as the blank control group, and Example 1 in Application No. CN202011309952.3, a method for inhibiting citrus Huanglongbing in citrus cultivation, was used as the positive control group to test the Huanglongbing pathogen on the leaves;

[0105] (3) Testing

[0106] (3.1) Antibiotic residues: Five trees were randomly tested in each group. After the last trunk infusion, citrus fruits were collected at different times (7d, 25d, 60d and 73d) during the fruit ripening period to detect the degradation of antibiotic residues in the fruits. The test results are shown in Table 1.

[0107] (3.2) Huanglongbing pathogen on leaves: 20 trees in each group were selected as test objects, totaling 180 trees. 20 leaf samples were collected from each plant for DNA extraction, and real-time fluorescence quantitative PCR was used to determine the Ct value of Huanglongbing pathogen on the plants. The real-time fluorescence quantitative PCR experiment was based on the literature Wenbin Li et al., 2006. The reaction system was 20 μL, the final concentration of the primer was 250 nM, the final concentration of the probe was 150 nM, and the buffer was based on the instructions of the Takara probe method real-time fluorescence quantitative PCR kit. PCR reaction procedure: 95℃ pre-denaturation for 20 s, then 95℃ for 5 s, 58℃ for 40 s, and 40 cycles. After the experiment, the Ct value of the fluorescence quantitative PCR reaction was recorded.

[0108] (3.2.1) Result determination: If the Ct value of the test sample is ≥40.0 or no Ct value is found, the sample is determined to be negative, indicating that the sample does not contain detectable Bacillus asiaticus species; if the Ct value of the sample is ≤35.0, the sample is determined to be positive, indicating that Bacillus asiaticus species is present in the sample; for samples with a Ct value greater than 35 but less than 40 in real-time fluorescence PCR detection, real-time fluorescence PCR re-testing is performed using primers for the nucleotide reductase gene that has 5 copies in the genome, and the results of the re-testing are used to determine whether the citrus Huanglongbing disease is present according to the above-mentioned determination criteria; if the Ct value is still greater than 35, it is determined to be negative.

[0109] Cure rate = (cured plants / 20) × 100%, the test results are shown in Table 2:

[0110] (3.3) Citrus fruit quality: The quality of the citrus fruits obtained by the treatment method of Example 3 and the blank control group was tested. The test results are shown in Table 3:

[0111] (4) Test results

[0112] Table 1 Degradation of antibiotic drug residues

[0113]

[0114] As can be seen from Table 1, ampicillin and amoxicillin components could not be detected in citrus fruits on the 25th and 60th days, indicating that the drug solution A used in this application has been completely degraded and absorbed and does not affect the quality and safety of the fruit.

[0115] Table 2 Leaf Huanglongbing pathogens

[0116]

[0117]

[0118]

[0119] As can be seen from Table 2, the cure rate of citrus Huanglongbing after treatment by the treatment methods of Examples 1 to 3 reached 95%, and the best effect was achieved in Example 3. In Example 3, 19 out of 20 Huanglongbing trees before treatment turned from positive to negative, and the positive Ct value became larger, and the content of Huanglongbing pathogens was less. Comparison between Example 3 and Comparative Example 1 shows that the trunk infusion using two needles can quickly deliver liquid A to the tree pathogens, ensuring that the liquid is more evenly distributed in the tree body, thereby improving the treatment effect and the growth recovery effect of the tree; comparison between Example 3 and Comparative Example 1 shows that the foliar spray uses liquids of different formulas, and the mixed use of pesticides with different killing mechanisms can complement each other, improve the prevention and control effect, and at the same time delay the development of drug resistance in pests and diseases. This is because the mixing of pesticides with different mechanisms of action can increase the diversity of prevention and control, reduce the adaptability of pathogens to a single pesticide, and further accelerate the repair effect; comparison between Example 3 and Comparative Examples 3 and 4 shows that only the application technology of trunk infusion + foliar spraying can maximize the inhibition of the growth of Asiatic bast Bacillus. The trunk infusion directly injects the liquid into the trunk of the plant to ensure that citrus can directly absorb these liquid components, thereby quickly and effectively improving the growth of citrus. Foliar spraying utilizes the stomata and epidermal cells of the leaves to absorb and quickly replenish the bactericidal solution, especially when the citrus leaves are damaged or the stomata are open, which can quickly provide necessary support for the growth of citrus. The combination of the two methods can promote the rapid recovery of citrus trees. By comparing Example 3 with Comparative Example 5, it can be seen that only by using the complete formula of the present invention can the cure rate of Huanglongbing be improved.

[0120] Table 3 Fruit quality

[0121]

[0122] As shown in Table 3, the citrus fruits obtained by the treatment method of Example 3 and the blank control have increased fruit brightness, yellowed peel color, and increased soluble solids. This indicates that the method used in the present application can not only repair citrus Huanglongbing trees well, but also improve the quality of citrus fruits and the economic value of citrus.

[0123] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for repairing citrus Huanglongbing trees, characterized in that: The following steps are involved: (1) Trunk infusion: Drill a hole 20 to 50 cm above the ground in the trunk of the tree and inject liquid A. Use two needles each time to inject liquid A. The two needles are located on opposite sides of the trunk and are injected at the same level. The medicinal solution A comprises the following raw materials in parts by weight: 30-45 parts of 500-fold liquid of 99.6% ampicillin, 15-25 parts of 500-fold liquid of 20% amoxicillin, 15-22 parts of 1000-fold liquid of 0.1% triacontanol, 10-18 parts of 600-fold liquid of 23% copper rosinate, 7-14 parts of 500-fold liquid of 80% mancozeb, 5-10 parts of 1000-fold liquid of 19% calcium magnesium trace water-soluble fertilizer, and 100-200 parts of water; (2) Foliar spray: 12 to 18 days after each trunk infusion, spray the leaves with liquid B, which is divided into B1, B2, B3 and B4; B1 is the first foliar spray solution, which includes the following raw materials in parts by weight: 22-35 parts of 200-fold solution of 1.26% cephalosporin acetate, 20-25 parts of 300-fold solution of 20% thiophanate-methyl, 8-20 parts of 2000-fold solution of 20% dinotefuran, and 50-150 parts of water; B2 is the second foliar spray solution, which includes the following raw materials in parts by weight: 8 to 15 parts of 1000 times solution of 50% chlorobromoisocyanuric acid, 7 to 12 parts of 500 times solution of 30% nonyl copper, 3 to 9 parts of 2000 times solution of 25% thiamethoxam, and 30 to 70 parts of water; B3 is the third foliar spray solution, which includes the following raw materials in parts by weight: 16-30 parts of 600-fold solution of 47% Spring Thunder·Royal Copper, 12-26 parts of 300-fold solution of 20% Thiazole Zinc, 5-13 parts of 2000-fold solution of 22% Sulfoxaflor, and 50-100 parts of water; The B4 is the fourth foliar spray solution, which includes the following raw materials in parts by weight: 10 to 17 parts of 750 times liquid of 80% ethoxypropynyl, 8 to 15 parts of 800 times liquid of 3% sinomenine, 7 to 12 parts of 3000 times liquid of 70% imidacloprid, and 60 to 90 parts of water.

2. A method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: Step (1) The first trunk infusion begins in mid-May or late May.

3. A method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: In step (1), one of the two needles reaches deep into the xylem of the tree, and the other needle penetrates deeply into the phloem.

4. A method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The dosage of the Chinese medicinal solution A in step (1) is 150-300 mL / plant, and the infusion is performed 4 times, with an interval of 20-30 days between each infusion.

5. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The medicinal solution A in step (1) comprises the following raw materials in parts by weight: 40 parts of 500 times liquid of 99.6% ampicillin, 20 parts of 500 times liquid of 20% amoxicillin, 20 parts of 1000 times liquid of 0.1% triacontanol, 15 parts of 600 times liquid of 23% copper rosinate, 10 parts of 500 times liquid of 80% mancozeb, 7 parts of 1000 times liquid of 19% calcium magnesium trace water-soluble fertilizer, and 180 parts of water.

6. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The spraying amount of the liquid B in step (2) is 10-25 mL / plant, and the leaves are sprayed 4 times in total.

7. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The B1 in step (2) is the first foliar spray solution, which includes the following raw materials in parts by weight: 30 parts of 200 times solution of 1.26% cefotaxime acetate, 22 parts of 300 times solution of 20% thiophanate-methyl, 15 parts of 2000 times solution of 20% dinotefuran, and 100 parts of water.

8. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The B2 described in step (2) is the second foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 1000 times liquid of 50% chlorobromoisocyanuric acid, 10 parts of 500 times liquid of 30% nonyl copper, 6 parts of 2000 times liquid of 25% thiamethoxam, and 60 parts of water.

9. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The B3 in step (2) is the third foliar spray solution, which includes the following raw materials in parts by weight: 28 parts of a 600-fold solution of 47% spring thunder·copper king, 19 parts of a 300-fold solution of 20% thiabendazole zinc, 10 parts of a 2000-fold solution of 22% sulfoxaflor, and 70 parts of water.

10. The method for repairing citrus Huanglongbing trees according to claim 1, characterized in that: The B4 described in step (2) is the fourth foliar spray solution, which includes the following raw materials in parts by weight: 12 parts of 750 times liquid of 80% ethionine by mass concentration, 13 parts of 800 times liquid of 3% sinomenine by mass concentration, 10 parts of 3000 times liquid of 70% imidacloprid by mass concentration, and 80 parts of water.

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