Ultra-trace solution microwire manufacturing system and method based on spiral structure guiding liquid

Abstract

The invention belongs to the technical field of ultramicro solution distribution, and particularly relates to an ultramicro solution microwire manufacturing system and method based on spiral structure guided liquid. Requirements for ultramicro solution dispensing techniques with high precision, high stability and high efficiency appear increasingly in various fields. The invention provides an ultramicro solution microwire manufacturing system and method based on spiral structure guided liquid by combining a transfer printing type solution distribution mode and a piston type solution distribution mode. Accurate pressure supply of a solution is achieved through the micro-fluidic injection pump, the solution is conveyed into the solution non-mechanism liquid storage barrel, the needle head and the spiral pipetting needle are arranged at the bottom of the liquid storage barrel, spiral grooves are evenly formed in the spiral pipetting needle, and the solution flows out through a gap between the needle head and the spiral pipetting needle; and the solution is flatly laid in the spiral groove on the spiral pipetting needle and spirally slides down at a constant speed, so that liquid drops are not easy to form, continuous supply of the solution can be realized, and manufacturing of ultramicro micron wires is realized.

Description

Technical Field

[0001] The invention belongs to the technical field of ultra-trace solution distribution, and in particular relates to an ultra-trace solution micrometer wire manufacturing system and method based on a spiral structure to guide liquid. Background Art

[0002] With the development of microsystem integrated assembly technology, the level of packaging, connection and sealing of ultra-precision parts at the micro-nano level has reached the femtoliter-picoliter level, which puts forward higher requirements for packaging accuracy, dosage and efficiency; with the development of bioanalysis technology and high-throughput screening technology, the dosage of reagents in the field of biological sciences has reached the nanoliter or even picoliter level, which puts forward higher requirements for the speed and accuracy of reagent distribution. The demand for ultra-micro solution distribution technology with high precision, high stability and high efficiency is becoming increasingly apparent in various fields. Existing technologies can no longer meet practical applications, and it is very necessary to study different types of solution distribution technologies and propose and develop new solution distribution technologies.

[0003] Existing solution dispensing technologies are mainly divided into two categories: contact type and non-contact type. Among the contact dispensing methods, the time-pressure type is the most widely used. However, in order to achieve ultra-micro solution dispensing, the hollow needle must be processed to a few microns or smaller, which makes manufacturing difficult. In addition, the flow resistance of high-viscosity solutions can easily cause needle blockage, resulting in large economic losses. The piston type has the advantages of good stability and easy control, but its maintenance and adjustment are difficult and expensive. Subsequently, a transfer dispensing method was proposed, in which the pipette needle passes through the capillary filled with solution, moves vertically downward until it contacts and stops with the base surface, and then moves upward along the original route. In this process, the solution at the tip of the needle forms droplets on the base surface under the action of the viscosity, inertia and surface tension of the solution itself. This method is applicable to a wide range of solution viscosities and has good consistency in the transferred droplets, but this method cannot achieve continuous supply of solution, that is, it cannot perform continuous solution dispensing, and it cannot perform micron-level linear dispensing, and the transfer and dispensing efficiency is low. The non-contact dispensing method is to use external force to make the solution break through the surface tension and spray it onto the base surface at a certain speed to form droplets. This method only requires horizontal movement and the nozzle does not need to contact the substrate. Therefore, the non-contact solution transfer efficiency is higher than the contact solution transfer and it is not easy to be polluted. However, the transfer amount and position accuracy of the non-contact solution transfer are not easy to control.

[0004] The existing solution distribution method is difficult to achieve a wide range of applicable viscosities, continuous supply of ultra-trace solutions, and cannot realize the manufacture of micrometer wires, which cannot meet the ultra-trace use requirements in various fields. Therefore, there is a need for an ultra-trace solution distribution and ultra-fine micrometer wire manufacturing technology that can ensure high precision, high stability and high efficiency of the transferred solution, as well as controllable continuous supply; there is a need for a new type of pipette needle that can achieve continuous and stable flow of solution on its surface. Summary of the invention

[0005] The purpose of the present invention is to provide a system and method for manufacturing ultra-trace solution micrometer wires based on a spiral structure to guide the flow of liquid, which can achieve continuous distribution of ultra-trace solutions and stable lines. The present invention is a composite ultra-trace solution micrometer wire manufacturing method that combines transfer-type and piston-type solution distribution. A pipette needle with a spiral structure is designed, and the spiral structure is used to guide the solution to flow on the surface of the pipette needle to achieve the purpose of continuous supply of solution, realize the manufacture of ultra-trace solution micrometer wires, and improve the solution transfer efficiency; the composite ultra-trace solution micrometer wire manufacturing method is intended to achieve performance quality indicators, clear contours, and the micrometer wire width can be controlled at the submicron level.

[0006] To achieve the above-mentioned purpose, the present invention adopts the following specific scheme: the ultra-trace solution micrometer wire manufacturing system proposed in the present invention specifically comprises a base and a microfluidic injection pump with a syringe, a vertical lifting mechanism is arranged on the side of the base, the lifting component of the vertical lifting mechanism is connected with a clamping mechanism, and a solution dispensing mechanism is arranged through the clamping mechanism; The solution dispensing mechanism includes a liquid storage cylinder, a needle is arranged at the bottom of the liquid storage cylinder, a spiral pipette needle is arranged on the needle, a fixing part is connected to the top of the liquid storage cylinder, a three-jaw chuck and a reducing tube are arranged through the fixing part, the three-jaw chuck clamps the upper end of the spiral pipette needle, and the upper end opening of the reducing tube is connected to the liquid outlet of the syringe provided by the microfluidic injection pump through a pipeline.

[0007] As a preferred solution of the present invention, the vertical lifting mechanism includes two parallel slide rails arranged along the vertical direction on the side of the base, a vertical through groove is arranged on the base between the two parallel slide rails, two sliders are arranged through the two slide rails, the two sliders are fixedly connected to the clamping mechanism, a cylinder is arranged on the base, and the telescopic rod of the cylinder is connected to the clamping mechanism through the through groove.

[0008] As another preferred embodiment of the present invention, the clamping mechanism includes a fixing plate, the fixing plate is connected to the telescopic rod of the cylinder through the through slot by a nut, the fixing plate 8 is fastened to the two sliders 6 by screws, a side plate is arranged in parallel at each of the two horizontal ends of the fixing plate, a parallel lead screw, a first guide shaft and a second guide shaft are arranged between the two side plates, two clamping blocks are symmetrically arranged through the first guide shaft, the lead screw and the second guide shaft, and V-shaped clamping grooves are arranged on opposite sides of the two clamping blocks; The threads at both ends of the lead screw are arranged in opposite directions, a bearing and a retaining spring are arranged between the lead screw and the side plate, and a hexagonal groove is arranged on the side of one end of the lead screw for easy insertion and rotation of a wrench.

[0009] As a third preferred solution of the present invention, a first sealing ring is provided between the fixing member and the reducer, and a second sealing ring is provided between the fixing member and the liquid storage cylinder.

[0010] The method for manufacturing ultra-trace solution micrometer wires based on the above system specifically comprises the following steps: Step 1: Select the needle and spiral pipette needle of corresponding specifications according to the required solution line size and solution viscosity, and install the spiral pipette needle in the three-jaw chuck to ensure its precise positioning in subsequent operations; Step 2: Control the telescopic rod of the cylinder to work, drive the clamping mechanism and the solution dispensing mechanism to move in the vertical direction, so that the spiral pipette needle reaches the predetermined solution line manufacturing position, so as to form and deposit droplets at an appropriate height; Step 3: Set the relevant parameters of the microfluidic syringe pump and start the microfluidic syringe pump. The solution enters the reservoir through the pipeline, and the solution flows out of the reservoir from the gap between the needle and the spiral pipette needle. The spiral groove on the spiral pipette needle guides the solution to move in a uniform spiral motion in the groove, and finally forms an initial droplet at the tip of the spiral pipette needle. Step 4: Control the substrate to move horizontally along a predetermined trajectory. A liquid bridge is formed between the tip of the spiral pipette needle and the substrate due to the surface tension and adhesion of the droplet. The continuous movement of the substrate stretches the liquid bridge to produce ultra-micro and ultra-fine solution micrometer lines. Step 5: After the substrate completes the horizontal lateral movement according to the predetermined trajectory, the vertical lifting mechanism is controlled to drive the solution dispensing mechanism to rise, and the microfluidic injection pump is controlled to perform a suction operation to effectively suck back the residual solution at the needle, thereby avoiding liquid solidification blockage and ensuring the accuracy of subsequent operations.

[0011] Beneficial effects of the present invention: (1) The present invention combines the advantages of the piston type in terms of stability, sealing and flow stability and the transfer type in terms of high precision, solving the problem of difficult control of the liquid output and inability to achieve stable and accurate continuous flow supply, and realizing the manufacture of ultra-micro and ultra-fine solution micrometer lines that can accurately control the liquid output and stabilize the liquid supply; (2) The present invention uses the spiral structure on the spiral pipette needle to play a role in fluid guidance, optimize the flow path of the fluid, and transform the linear falling motion of the solution along the pipette needle into a uniform spiral motion, thereby improving the transfer efficiency of the glue solution; the design of the spiral structure can also effectively prevent the solution from gathering into droplets at the outlet of the needle tube, thereby improving the flow efficiency of the solution. The spiral structure increases the contact area between the solution and the pipette needle, and can more stably control the flow of the solution; (3) The present invention can realize the manufacture of ultra-micro and ultra-fine solution micrometer lines, and is also suitable for solution distribution in various ways such as points, lines and surfaces; (4) The ultra-trace solution micrometer wire manufacturing system proposed in the present invention can adapt to different sizes of liquid storage cylinders by providing a clamping mechanism, and the three-jaw chuck is provided to facilitate the replacement of the pipette needle; (5) The ultra-trace solution micrometer wire manufacturing method based on the spiral structure guiding the liquid can ultimately stably manufacture solution wires with a width of about ten micrometers. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 This is a diagram of the method for manufacturing ultra-trace solution micrometer wires.

[0013] Figure 2 Schematic diagram of the fabrication principle of ultra-trace solution micrometer wires.

[0014] Figure 3 Schematic diagram of the structure of the spiral pipette needle.

[0015] Figure 4 This is a comparison chart of the actual situation between traditional tungsten needles and spiral pipette needles in achieving continuous solution supply.

[0016] Figure 5 This is a force analysis diagram of the glue flow in the spiral structure of the spiral pipette needle.

[0017] Figure 6 Schematic diagram of the overall structure of the ultra-trace solution micrometer wire manufacturing system.

[0018] Figure 7 Schematic diagram of the vertical lifting mechanism.

[0019] Figure 8 It is a schematic diagram of the clamping mechanism structure.

[0020] Fig. 9 Schematic diagram of the lead screw structure.

[0021] Fig.10 Schematic diagram of the solution distribution mechanism structure.

[0022] Fig.11 This is the fixed part axis view.

[0023] Fig.12 A top view of the fixing.

[0024] Fig.13 Schematic diagram of the fixing assembly structure.

[0025] Fig.14 Schematic diagram of solution micrometer wires obtained by the present invention.

[0026] In the accompanying drawings, 1 is a microfluidic injection pump, 2 is a clamping mechanism, 3 is a vertical lifting mechanism, 4 is a solution dispensing mechanism, 5 is a slide rail, 6 is a slider, 7 is a nut, 8 is a fixed plate, 9 is a cylinder, 10 is a base, 11 is a side plate, 12 is a bearing, 13 is a retaining spring, 14 is a first guide shaft, 15 is a lead screw, 16 is a second guide shaft, 17 is a clamping block, 18 is a reducer, 19 is a first sealing ring, 20 is a three-jaw chuck, 21 is a fixing part, 22 is a second sealing ring, 23 is a liquid storage cylinder, 24 is a needle, and 25 is a spiral pipette needle. DETAILED DESCRIPTION

[0027] The ultra-trace solution micrometer wire manufacturing system and method proposed by the present invention are described in detail below with reference to the accompanying drawings.

[0028] like Figure 1 As shown, the method for manufacturing ultra-trace solution micrometer wires proposed by the present invention is a composite method for manufacturing ultra-trace solution micrometer wires that combines transfer-type and piston-type solution distribution.

[0029] like Figure 2 As shown, the method for manufacturing ultra-trace solution micrometer wires proposed by the present invention includes four stages: solution extrusion, liquid bridge connection, translation into wires, and solution back suction.

[0030] In order to achieve the above technical effects, the present invention proposes an ultra-trace solution micron wire manufacturing system, such as Figure 6 As shown, it specifically includes a base 10 and a microfluidic injection pump 1 with a syringe. The microfluidic injection pump 1 is a precise, low-flow injection pump. The microfluidic injection pump 1 includes a syringe base, an operation screen, a syringe clamp that is tightly gripped and safer, and a syringe. The flow value is input in the operation screen, and the injection pump is started, which can generate precise pressure to push the syringe. The top of the syringe is connected to a pump tube to supply pressure to the solution. A vertical lifting mechanism 3 is disposed on the side of the base 10, and a lifting component of the vertical lifting mechanism 3 is connected to a clamping mechanism 2, and a solution dispensing mechanism 4 is disposed through the clamping mechanism 2; like Fig.10 As shown, the solution dispensing mechanism 4 includes a liquid storage cylinder 23, a needle 24 is arranged at the bottom of the liquid storage cylinder 23, a spiral pipette needle 25 is arranged on the needle 21, and a fixing member 21 with a through hole is connected to the top of the liquid storage cylinder 23, and a three-jaw chuck 20 and a reducer 18 are arranged through the fixing member 21. The three-jaw chuck 20 clamps the upper end of the spiral pipette needle 25, and the upper end opening of the reducer 18 and the liquid outlet of the built-in syringe of the microfluidic injection pump 1 are connected through a pipeline; needles 24 of different specifications can be replaced according to the viscosity of the solution, and the gap between the needle 24 and the spiral pipette needle 25 is changed to prevent the solution from being blocked or flowing independently.

[0031] The structure of the fixing member 21 is as follows: Fig.11 and Fig.12 As shown, the fixing part 21 with a through hole can simultaneously fix the three-jaw chuck 20, connect the reducer 18, connect the liquid storage cylinder 23, and seal the solution; a racetrack-shaped through hole is arranged inside the fixing part 21, which can increase the flow area of ​​the solution, and the regular shape is not easy to be blocked, and it is a key part.

[0032] Fig.13 The installation and tolerance requirements of the fixing part 21 with a through hole are as follows. As a key part, the fixing part 21 with a through hole realizes multiple functions such as fixing, connection and sealing at the same time, so the production and manufacturing of this part is required to have certain precision requirements; the precision of the fixing part 21 also directly affects the concentricity of the spiral pipette needle 25 with the needle head 24 after installation and reasonably controls the gap to avoid the occurrence of solution leakage.

[0033] The spiral pipette needle 25 has a structure as follows: Figure 3 As shown, the spiral pipette needle 25 is one of the core parts of the method for manufacturing ultra-trace solution micrometer wires; the manufacturing process of the spiral pipette needle 25 is as follows: a cylindrical substrate with suitable material and size is selected, and the surface is polished to be smooth and flat; on this substrate, a groove with a certain depth and width is cut along a spiral path, and finally a conical tip with a diameter of micrometer level is processed at the end of the substrate by a fine grinding process.

[0034] like Figure 3 As shown, the installation of the spiral pipette needle 25 must ensure parallelism with the base surface, concentricity with the needle head 24, and reasonable control of the gap to avoid glue leakage.

[0035] Figure 4 The actual situation of achieving continuous supply of solution between the traditional cylindrical pipette needle and the spiral pipette needle 25 is compared; the traditional cylindrical pipette needle cannot achieve continuous supply of solution, and the "sugar gourd phenomenon" occurs, the solution gathers into droplets, and drips under the action of gravity, and continuous flow of solution cannot be achieved; when the spiral pipette needle 25 is used to supply the solution, the spiral structure increases the contact area between the solution and the pipette needle, so that the solution flows along the spiral structure, which can effectively prevent the solution from gathering into droplets at the needle tube outlet, thereby achieving continuous supply of solution and improving the flow efficiency of the glue solution.

[0036] Figure 5The flow state of the glue solution in the spiral structure is demonstrated. The existence of the spiral groove affects the flow of the glue, making it have greater resistance in the direction perpendicular to the spiral groove, and the droplets are not easy to spread; there are adhesion and shear effects between the wall of the spiral groove and the glue. The introduction of the spiral groove increases the actual contact area between the solid and the liquid, increases the interaction force between the solid and the liquid, and increases the adhesion force, thereby reducing the contact angle and weakening the rolling property of the droplets; the spiral groove guides the glue, and the movement resistance of the droplets parallel to the spiral groove is small, and the droplets are easy to spread. At this time, gravity G is the main driving force for the glue to flow in the spiral groove, acting on each unit volume of the liquid to generate downward pressure, and the inclination angle of the spiral groove directly affects the component of gravity; the wall and inclination angle of the spiral groove will cause the glue to generate an inertial force FA along the direction of the spiral groove; the roughness of the wall of the spiral groove of the glue will generate a friction force FL that affects the flow of the liquid, thereby affecting the flow direction and speed of the liquid.

[0037] like Figure 7 As shown, the vertical lifting mechanism 3 includes two parallel slide rails 5 arranged along the vertical direction on the side of the base 10, a vertical through groove is arranged on the base 10 between the two parallel slide rails 5, two sliders 6 are arranged through the two slide rails 5, the two sliders 6 are fixedly connected to the clamping mechanism 2, and a cylinder 9 is arranged on the base 10, and the telescopic rod of the cylinder 9 is connected to the clamping mechanism 2 through the through groove.

[0038] like Figure 8 As shown, the clamping mechanism 2 includes a fixing plate 8, which is connected to the telescopic rod of the cylinder 9 through the through slot by a nut 7, and is fastened to the two sliders 6 by screws. A side plate 11 is arranged in parallel at each of the two horizontal ends of the fixing plate 8, and a parallel lead screw 15, a first guide shaft 14 and a second guide shaft 16 are arranged between the two side plates 11. Two clamping blocks 17 are symmetrically arranged through the first guide shaft 14, the lead screw 15 and the second guide shaft 16, and V-shaped clamping grooves are arranged on opposite sides of the two clamping blocks 17; like Fig. 9 As shown, the threads at both ends of the lead screw 15 are set in opposite directions, a bearing 12 and a retaining spring 13 are set between the lead screw 15 and the side plate 11, and a hexagonal groove is set on the side of one end of the lead screw 15 to facilitate the insertion and rotation of a wrench; the lead screw 15 can be rotated by inserting a wrench to adjust the distance between the two clamping blocks 17, which is used to clamp liquid storage cylinders 23 of different sizes.

[0039] As a third preferred solution of the present invention, a first sealing ring 19 is provided between the fixing member 21 and the reducer 18 , and a second sealing ring 22 is provided between the fixing member 21 and the liquid storage cylinder 23 .

[0040] The method for manufacturing ultra-trace solution micrometer wires based on the above system specifically comprises the following steps: Step 1: Select the needle 24 and spiral pipette needle 25 of corresponding specifications according to the required solution line size and solution viscosity, and install the spiral pipette needle 25 in the three-jaw chuck 20 to ensure its accurate positioning in subsequent operations; Step 2: Control the telescopic rod of the air cylinder 9 to work, drive the clamping mechanism 2 and the solution dispensing mechanism 4 to move in the vertical direction, so that the spiral pipette needle 25 reaches the predetermined solution line manufacturing position, so as to form and deposit droplets at an appropriate height; Step 3: Set the relevant parameters of the microfluidic injection pump 1 and start the microfluidic injection pump 1. The solution enters the liquid storage cylinder 23 through the pipeline, and the solution flows out of the liquid storage cylinder 23 from the gap between the needle 24 and the spiral pipette needle 25. The spiral groove on the spiral pipette needle 25 guides the solution to move in a uniform spiral motion in the groove, and finally forms an initial droplet at the tip of the spiral pipette needle 25; Step 4: Control the substrate to move along a predetermined trajectory in the horizontal direction. A liquid bridge is formed between the tip of the spiral pipette needle 25 and the substrate due to the surface tension and adhesion of the droplet. The continuous movement of the substrate stretches the liquid bridge to produce ultra-micro and ultra-fine solution micrometer lines to meet specific application requirements. Step 5: After the substrate completes the horizontal lateral movement according to the predetermined trajectory, the vertical lifting mechanism 3 is controlled to drive the solution dispensing mechanism 4 to rise, and the microfluidic injection pump 1 is controlled to perform a back suction operation to effectively suck back the residual solution at the needle tip, thereby avoiding liquid solidification blockage and ensuring the accuracy of subsequent operations.

[0041] The continuous supply of solution cooperates with the movement of the spiral pipette needle 25. Part of the solution spreads and adheres to the base surface by virtue of adhesion and surface tension. Combined with the transfer principle, the distribution of ultra-trace solution is completed. At the same time, the motion control of the solution distribution mechanism 4 is used to realize the manufacture of ultra-fine and continuous micrometer wires.

[0042] like Fig.14 As shown, Fig.14 In order to apply the ultra-trace solution micrometer wire manufacturing method based on spiral structure guiding liquid proposed in the present invention, the solution wire packaging experiment results were conducted. The experiment used methyl silicone oil with a viscosity of 1000mPa.s as the experimental solution.

[0043] It can be understood that the above specific description of the present invention is only used to illustrate the present invention and is not limited to the technical solutions described in the embodiments of the present invention. Those skilled in the art should understand that the present invention can still be modified or replaced by equivalents to achieve the same technical effects; as long as the use requirements are met, they are within the protection scope of the present invention.

Claims

1. A system for manufacturing ultra-trace solution micrometer wires based on a spiral structure to guide liquid, characterized in that: It comprises a base (10) and a microfluidic injection pump (1) with a syringe, wherein a vertical lifting mechanism (3) is arranged on the side of the base (10), a lifting component of the vertical lifting mechanism (3) is connected to a clamping mechanism (2), and a solution dispensing mechanism (4) is arranged through the clamping mechanism (2); The solution dispensing mechanism (4) comprises a liquid storage cylinder (23), a needle (24) is arranged at the bottom of the liquid storage cylinder (23), a spiral pipette needle (25) is arranged on the needle (21), and spiral grooves are evenly arranged on the spiral pipette needle (25). A fixing member (21) is connected to the top of the liquid storage cylinder (23), and a three-jaw chuck (20) and a reducer (18) are arranged through the fixing member (21). The three-jaw chuck (20) clamps the upper end of the spiral pipette needle (25), and the upper end opening of the reducer (18) is connected to the liquid outlet of the syringe provided by the microfluidic injection pump (1) through a pipeline.

2. The ultra-trace solution micrometer wire manufacturing system based on spiral structure guiding liquid as claimed in claim 1, characterized in that: The vertical lifting mechanism (3) comprises two parallel slide rails (5) arranged along the vertical direction on the side of the base (10), a vertical through slot is arranged on the base (10) between the two parallel slide rails (5), two sliders (6) are arranged through the two slide rails (5), the two sliders (6) are fixedly connected to the clamping mechanism (2), a cylinder (9) is arranged on the base (10), and a telescopic rod of the cylinder (9) is connected to the clamping mechanism (2) through the through slot.

3. The ultra-trace solution micrometer wire manufacturing system based on spiral structure guiding liquid as claimed in claim 2, characterized in that: The clamping mechanism (2) comprises a fixing plate (8), the fixing plate (8) being connected to the telescopic rod of the cylinder (9) through the through slot via a nut (7), the fixing plate 8 being fastened to the two slide blocks 6 via screws, a side plate (11) being arranged in parallel at each of the two horizontal ends of the fixing plate (8), a parallel lead screw (15), a first guide shaft (14) and a second guide shaft (16) being arranged between the two side plates (11), two clamping blocks (17) being symmetrically arranged via the first guide shaft (14), the lead screw (15) and the second guide shaft (16), and V-shaped clamping grooves being arranged on opposite sides of the two clamping blocks (17); The threads at both ends of the lead screw (15) are arranged in opposite directions, a bearing (12) and a retaining ring (13) are arranged between the lead screw (15) and the side plate (11), and a hexagonal groove is arranged on the side surface of one end of the lead screw (15) for facilitating the insertion and rotation of a wrench.

4. The ultra-trace solution micrometer wire manufacturing system based on spiral structure guiding liquid as claimed in claim 1, characterized in that: A first sealing ring (19) is provided between the fixing member (21) and the reducing tube (18), and a second sealing ring (22) is provided between the fixing member (21) and the liquid storage cylinder (23).

5. A method for manufacturing ultra-trace solution micrometer wires based on a spiral structure guiding liquid, characterized in that: The specific steps include: Step 1: Select a needle (24) and a spiral pipette needle (25) of corresponding specifications according to the required solution line size and solution viscosity, and install the spiral pipette needle (25) in the three-jaw chuck (20); Step 2: Control the telescopic rod of the air cylinder (9) to work, drive the clamping mechanism (2) and the solution dispensing mechanism (4) to move in the vertical direction, so that the spiral pipette needle (25) reaches a predetermined solution line manufacturing position; Step 3: Setting relevant parameters of the microfluidic injection pump (1) and starting the microfluidic injection pump (1), the solution enters the liquid storage cylinder (23) through the pipeline, and the solution flows out of the liquid storage cylinder (23) from the gap between the needle head (24) and the spiral pipette needle (25), and the spiral groove on the spiral pipette needle (25) guides the solution to move in a uniform spiral motion in the groove, and finally forms an initial droplet at the tip of the spiral pipette needle (25); Step 4: Control the substrate to move along a predetermined trajectory in the horizontal direction, and a liquid bridge is formed between the tip of the spiral pipette needle (25) and the substrate due to the surface tension and adhesion of the liquid droplet. The continuous movement of the substrate stretches the liquid bridge to produce ultra-micro and ultra-fine solution micrometer lines; Step 5: After the substrate has completed horizontal lateral movement according to a predetermined trajectory, the vertical lifting mechanism (3) is controlled to drive the solution dispensing mechanism (4) to rise, and the microfluidic injection pump (1) is controlled to perform a back suction operation.

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