Microorganism concentration detection device and method
By adopting the design of inclined positioning chute and light detector in the microbial concentration detection device, the inconsistency and damage of light source distances caused by different culture bottle sizes is solved, and higher detection consistency and accuracy are achieved.
Patent Information
- Application Number
- CN202411362832.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-27
- Publication Date
- 2024-12-20
AI Technical Summary
The existing microbial concentration detection devices may easily lead to inconsistent light source distances when the size of the culture bottle is different, causing detection errors, and the culture bottle may be damaged during the positioning process.
Design a microbial concentration detection device, adopting an inclined distribution positioning chute and light detector, through gravity positioning and curved surface structure, ensure that the culture bottle is stable in an inclined posture, the distance between the light source and the side wall of the culture bottle is consistent, and the extrusion and damage are avoided. And optimize the light source layout to reduce background noise.
It improves detection consistency and accuracy, reduces detection errors, and adapts to culture bottles of different sizes to avoid damage to culture bottles and meets the measurement needs of a small amount of liquids.
Smart Images
Figure CN119161972A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of microorganism detection, and in particular to a device and method for detecting microorganism concentration. Background Art
[0002] When monitoring the concentration of cultured microorganisms, the microbiology laboratory needs to open the sealed culture bottle, use a sterile pipette to carefully aspirate a certain amount of microbial culture solution, dilute it if necessary, and pour it into a cuvette, place the cuvette in the sample chamber of the spectrophotometer, measure according to the instrument operation guide, and determine the microbial concentration value based on the standard curve and the measurement results; if the concentration does not meet the standard, it needs to be re-sealed and cultured; when opening the sealed culture bottle, phage contamination is likely to be introduced, resulting in culture failure.
[0003] A Chinese patent (publication number: CN114062321A) discloses a rapid microbial concentration tester that can detect the concentration of the internal culture solution in a sealed culture bottle. The culture bottle and the internal culture solution are illuminated by detection light, and the field aperture receives the reflected light, thereby calculating the concentration value in the culture bottle based on the reflected light signal; and an arc block is configured to clamp and fix the reagent cup, and the reagent cup can be rotated to adjust its position, so that detection can be performed from multiple directions to improve detection accuracy. A rubber pad and a corresponding arc groove are provided on the inner side of the arc block, but when culture bottles of different sizes are clamped, there are still positions where the curvature of the outer wall of the culture bottle and the curvature of the arc groove are inconsistent. In the process of the arc block moving to push the culture bottle to adjust its position, it is easy for the culture bottle to contact the arc groove to form a point contact. During the positioning process, there is a situation where stress concentration causes damage; in addition, after the arc block pushes the culture bottle to move, it is in a position coaxial with the circle determined by the arc block. The distances between culture bottles of different sizes and the light detector are different, which makes it difficult for the light detector and different culture bottles to be within a suitable light source distance during the test, resulting in poor consistency in light distance during the measurement of culture bottles of different sizes, resulting in large errors introduced into the detection process. Summary of the invention
[0004] The purpose of the present invention is to provide a microorganism concentration detection device and method in view of the defects of the prior art. An inclined positioning slide groove is provided on the base. After the culture bottle to be tested is placed in the positioning slide groove, it can maintain an inclined and stable posture to achieve gravity positioning and avoid the problem of squeezing and breakage of the culture bottle. At the same time, after the culture bottle reaches the limit position, the side of the culture bottle facing the light detector is in a nearly vertical posture that is convenient for detecting the injection of light, which is convenient for the transmission detection of the detection light and also makes the distance between the side of the culture bottles of different sizes facing the light detector and the light detector close, thereby facilitating the control of the light source distance, improving the detection consistency and reducing the detection error.
[0005] The first object of the present invention is to provide a microorganism concentration detection device, which adopts the following scheme:
[0006] include:
[0007] A base, on which an inclined guide slot is provided, wherein one end of the guide slot lower than the bottom surface of the base is a blocking end, and the other end is an open end, the blocking end forms a positioning surface abutting against the side wall of the culture bottle, and the bottom surface of the guide slot serves as a bearing surface contacting the bottom wall of the culture bottle;
[0008] The light detector includes a light source and a light receiver facing the positioning slide groove. The vertical bisector of the connecting line of the guide side surfaces on both sides of the guide slide groove is used as the reference plane. The distribution positions of the light source and the light receiver are coplanar with the reference plane.
[0009] Furthermore, the positioning surface is a curved surface, and two ends of the curved surface are respectively connected to the guide side surfaces.
[0010] Furthermore, the trajectory of the positioning surface is in an arc shape, and the projection shape of the positioning surface with the bearing surface as the projection surface is in an arc shape.
[0011] Furthermore, the generatrix of the side wall of the culture bottle carried by the carrying surface is perpendicular to the reflected light, so that the light is captured vertically or obliquely relative to the side wall of the culture bottle.
[0012] Furthermore, the contact position between the culture bottle and the positioning surface is symmetrical with respect to the reference surface, or the contact position is coplanar with the reference surface.
[0013] Furthermore, the culture bottle is in surface contact with the positioning surface, and the contact surface is a symmetrical structure relative to the reference surface, or the culture bottle is in point contact with the positioning surface, and the contact point is coplanar with the reference surface.
[0014] Furthermore, the light sources of the light detector are located on the same side of the guide slot.
[0015] Furthermore, the optical detector is connected to a controller, the optical detector is used to emit detection light and receive reflected light, and the controller is used to control the optical detector to emit detection light and obtain reflected light information received by the optical detector.
[0016] The second object of the present invention is to provide a test method for the microorganism concentration detection device as described in the first object, comprising:
[0017] Take the sealed culture bottle and slide it into the guide slot along the open section until the culture bottle abuts against the sealed end and stabilizes. The culture bottle is tilted so that the culture liquid level in the culture bottle is higher than the light detector.
[0018] The light source of the light detector outputs the detection light to enter the culture bottle vertically or obliquely relative to the side wall of the culture bottle; the light receiver collects the detection light reflected from the culture liquid and converts it into an electrical signal;
[0019] The controller acquires and processes the electrical signal and calculates the microbial concentration of the culture solution in the culture bottle.
[0020] Furthermore, the culture bottle forms surface contact or point contact with the positioning surface, so that when different culture bottles are detected, the distance between the incident point of the light source and the detection light on the side wall of the culture bottle is equal.
[0021] Compared with the prior art, the present invention has the following advantages and positive effects:
[0022] (1) In order to solve the problem of large detection errors caused by the horizontal placement of culture bottles, an inclined positioning slot is provided on the base. After the culture bottle to be tested is placed in the positioning slot, it can maintain an inclined and stable posture to achieve gravity positioning and avoid the problem of squeezing and damage to the culture bottle. At the same time, after the culture bottle reaches the limit position, the side of the culture bottle facing the light detector is in a nearly vertical posture that is convenient for the detection light to enter, which is convenient for the transmission detection of the detection light and also makes the side of the culture bottles of different sizes facing the light detector close to the distance between the light detector, thereby facilitating the control of the light source distance, improving the detection consistency, and reducing the detection error.
[0023] (2) After the positioning slide is tilted, the relatively lower end is the blocking end, where the light detector can be arranged, and the relatively higher end is the entrance end, where the culture bottle can be placed. After moving along the positioning slide to the blocking end, the blocking end and the positioning slide limit the positioning to achieve positioning, and gravity and limit structures are used to ensure the stability of the detection state.
[0024] (3) The plugging end adopts a curved surface structure. When the bottom edge of the culture bottle contacts the plugging end, for culture bottles with matching diameters, the bottom of the culture bottle forms surface-to-surface contact with the plugging end, thereby ensuring the centering of the culture bottle. For culture bottles with a diameter smaller than that of the positioning slide, the bottom of the culture bottle forms point contact with the plugging end, and also forms a limiting state. The centering of the culture bottle and the positioning slide is achieved by using the guidance of the curved surface and the effect of gravity, so that culture bottles of different sizes can be adapted to improve its versatility. In addition, the culture bottle's own weight will not cause stress concentration at the point contact position, thereby avoiding damage to the culture bottle.
[0025] (4) After the culture bottle reaches a stable posture in the positioning slide, the wall of the culture bottle facing the light source approaches a vertical state, and the light emitted by the light source is transmitted into the culture bottle nearly perpendicular to the container wall. When the light is incident vertically, the light path is relatively short and direct, and the background noise (such as scattered light from the container wall) is relatively small, making the detected scattered light more likely to come from microbial particles, thereby improving the accuracy of the test.
[0026] (5) After the culture bottle is tilted and placed in the positioning slide, the liquid level inside is higher than when it is placed flat. For some small amounts of culture fluid, the height may be insufficient when placed flat, and the light source may not be directly irradiated, or it may be greatly affected by the bottom edge. However, after being tilted, the liquid level rises, allowing the light source to penetrate into the culture fluid in the culture bottle. This can not only meet the measurement needs of a small amount of liquid, solve the problem of insufficient detection position, but also ensure measurement accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] The accompanying drawings in the specification, which constitute a part of the present invention, are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.
[0028] Figure 1 Schematic diagram of the microorganism concentration detection device in Examples 1 and 2 of the present invention.
[0029] Figure 2 Schematic diagram of the flow equalizing plate in Examples 1 and 2 of the present invention.
[0030] Among them, 1. base, 2. positioning slide groove, 3. guide side, 4. blocking end, 5. light detector, 6. reference surface, 7. positioning surface. DETAILED DESCRIPTION
[0031] Example 1
[0032] In a typical embodiment of the present invention, Figure 1-Figure 2 As shown, a microorganism concentration detection device is provided.
[0033] When testing the concentration of microorganisms, it is easy for the culture bottle to contact the arc groove to form a point contact. During the positioning process, the extrusion effect of pushing the culture bottle to move will cause stress concentration in the culture bottle, causing damage; and the consistency of the light distance during the measurement of culture bottles of different sizes is poor, resulting in a large error in the detection process. Based on this, the present embodiment provides a microorganism concentration detection device, on which an inclined positioning chute 2 is provided on the base 1. After the culture bottle to be tested is placed in the positioning chute 2, it can maintain an inclined and stable posture, realize gravity positioning, and avoid the problem of extrusion and damage to the culture bottle. In addition, the positioning surface 7 formed by the blocking end 4 of the inclined guide chute can position the culture bottle, and the contact surface between the culture bottle and the positioning surface 7 is the incident position of the light emitted by the light source, so that the distance between the side of the culture bottles of different sizes facing the light detector 5 and the light detector 5 is close, thereby facilitating the control of the light source distance, improving the detection consistency, and reducing the detection error.
[0034] like Figure 1As shown, a microorganism concentration detection device mainly includes a base 1 and a light detector 5. The base 1 is provided with an inclined guide groove. After the culture bottle is placed in the guide groove, it can move along the guide groove. The lower end of the guide groove relative to the bottom surface of the base 1 is a blocking end 4, and the other end is an open end. The blocking end 4 forms a positioning surface 7 that abuts the side wall of the culture bottle to prevent the culture bottle from further moving along the guide groove. The bottom surface of the guide groove serves as a bearing surface that contacts the bottom wall of the culture bottle.
[0035] Combination Figure 2 As shown, the guide groove on the base 1 is designed to be inclined so that the culture bottle can slide to a stable and inclined angle after being placed. The gravity is used so that the culture bottle can maintain a stable posture without an additional fixing device, avoiding the problem of extrusion and breakage caused by direct placement or improper fixing method.
[0036] One end of the guide chute is designed as a blocking end 4, which is used to abut against the side wall of the culture bottle as a positioning point; the other end is an open end, which is convenient for the culture bottle to be placed in. The blocking end 4 forms a positioning surface 7 to ensure that the culture bottle can be stably stopped when sliding to the end, and its inclination angle is fixed, which is conducive to subsequent optical detection.
[0037] The bottom surface of the guide slide groove serves as a bearing surface, directly contacts the bottom wall of the culture bottle, provides necessary supporting force, ensures that the culture bottle will not tip over in a tilted state, and can guide the sliding process.
[0038] The light detector 5 includes a light source and a light receiver facing the positioning chute 2. The light source and the light receiver are arranged on the same side of the guide chute and are coplanar with the perpendicular bisector (reference plane 6) of the line connecting the guide side surfaces 3 on both sides of the guide chute. No matter how the size of the culture bottle changes, the side facing the light detector 5 (usually the side wall or transparent cover of the culture bottle) remains consistent with the relative position of the light source and the light receiver, thereby simplifying the control of the light source distance and improving the consistency of detection.
[0039] When the light from the light source penetrates the microbial sample in the culture bottle, the intensity of the transmitted light will be affected by the concentration of the microorganisms. The transmitted light intensity signal received by the light receiver will be converted into an electrical signal, and then the concentration of the microorganisms will be analyzed by the data processing system. Since the positions of the light source and the light receiver are fixed and consistent with the relative position of the culture bottle, the detection error caused by the change of the light source distance can be significantly reduced.
[0040] The inclined guide chute and gravity positioning method avoid direct squeezing of the culture bottle by the traditional fixing device, reducing the risk of breakage. By ensuring that the relative position of culture bottles of different sizes is consistent with the light detector 5 during detection, the control of the light source distance is simplified, and the consistency and accuracy of detection are improved. The layout of the light source and the light receiver is optimized, the detection error caused by the change of the light source distance is reduced, and the reliability of the detection result is improved.
[0041] The blocked end of the positioning chute 2 forms a positioning surface 7, and a hole can be provided on the positioning surface 7 for the installed light detector 5 to emit and receive light. The distance between the light detector 5 and the container bottle in the positioning chute 2 can be adjusted by adjusting the position of the light detector 5.
[0042] It should be pointed out that the culture bottle is generally a triangular flask, and the bottom cross-sectional area of the triangular flask is larger than the mouth cross-sectional area. In terms of posture, after the culture bottle is tilted and placed in place, the internal liquid level is higher than the flat state. For some small amounts of culture fluid, the liquid level height may be insufficient when it is laid flat in the culture bottle, and the light emitted from the light source position cannot directly irradiate the culture fluid. If the light source setting position is moved down, it will be easily affected by the complex curved surface of the bottom edge of the culture bottle, resulting in difficulties in data collection and processing. In the present embodiment, the inclined guide chute on the base 1 can be used to place the culture bottle in an inclined posture, so that the liquid level in the culture bottle is higher than when it is laid flat, and the light source can be transmitted into the culture fluid, which can not only meet the measurement needs of a small amount of liquid, but also ensure the measurement accuracy.
[0043] In this embodiment, the light emitted by the light source is distributed obliquely relative to the generatrix where the side wall of the culture bottle carried by the carrying surface is located, and the reflected light received by the light receiver is perpendicular to the generatrix where the side wall of the culture bottle carried by the carrying surface is located.
[0044] The plane where the bottom wall of the culture bottle accommodated by the guide chute is located and the corresponding generatrix of the side wall are distributed at an angle. If the side wall of the culture bottle is used as the incident position and forms an acute angle or an obtuse angle with the light emitted by the light source, the background noise may increase significantly due to multiple reflections and refractions of the light inside the container. In this regard, in other optional embodiments, the generatrix where the side wall of the culture bottle carried by the carrying surface is located is perpendicular to the light emitted by the light source, so that the light is perpendicular to the side wall of the culture bottle and penetrates into the culture liquid in the culture bottle.
[0045] When the incident direction of the light emitted by the light source is perpendicular to the generatrix of the side wall of the culture bottle corresponding to the incident position, the background noise (such as scattered light from the container wall) is relatively small because the light path is relatively short and direct. This makes the detected scattered light more likely to come from microbial particles, thereby improving the accuracy of the test.
[0046] To adapt to the shape of the culture flask, such as Figure 1As shown, the positioning surface 7 is a curved surface, and the two ends of the curved surface are respectively connected to the guide side surface 3; the trajectory of the positioning surface 7 is an arc shape, and the projection shape of the positioning surface 7 with the bearing surface as the projection surface is an arc shape.
[0047] The blocking end 4 adopts a curved surface structure. When the bottom edge of the culture bottle contacts the blocking end 4, for culture bottles with matching diameters, the bottom of the culture bottle forms surface-to-surface contact with the blocking end 4, thereby ensuring the centering of the culture bottle. For culture bottles with a diameter smaller than the positioning slide groove 2, the bottom of the culture bottle forms point contact with the blocking end 4, and also forms a limiting state. The centering of the culture bottle and the positioning slide groove 2 is achieved by utilizing the guidance of the curved surface and the effect of gravity, and culture bottles of different sizes can be adapted to improve its versatility. Moreover, stress concentration will not occur at the point contact position under the weight of the culture bottle, thereby avoiding damage to the culture bottle.
[0048] In addition, the contact position of the culture bottle and the positioning surface 7 is symmetrical with respect to the reference surface 6 or the contact position is coplanar with the reference surface 6. When the size of the culture bottle matches the size of the positioning surface 7, when the culture bottle and the positioning surface 7 are in surface contact, the contact surface is a symmetrical structure with respect to the reference surface 6. When the size of the culture bottle is smaller than the size of the positioning surface 7, the culture bottle and the positioning surface 7 are in point contact, and the contact point is coplanar with the reference surface 6.
[0049] After the culture bottle reaches the limit position, the side of the culture bottle facing the light detector 5 is in a nearly vertical posture that is convenient for detecting the light entering, which is convenient for the transmission detection of the detection light. The distance between the limit contact position and the light detector 5 is fixed. When different culture bottles are replaced, the contact positions of different culture bottles and the positioning surface 7 are on the same vertical plane. At the same time, the contact position corresponds to the generatrix of the side wall of the culture bottle close to the vertical, that is, the vertical line where the contact position of the culture bottle and the positioning surface 7 is located is the incident position of the light emitted by the light source, so that the side of the culture bottles of different sizes facing the light detector 5 is close to the distance between the light detector 5, so as to facilitate the control of the light source distance, improve the detection consistency, and reduce the detection error.
[0050] The light detector 5 is provided with a light source and a light receiver. The light detector 5 is connected to a controller. The light detector 5 is used to emit detection light and receive reflected light. The controller is used to control the light detector 5 to emit detection light and obtain reflected light information received by the light detector 5.
[0051] In this embodiment, the light source can be a laser source or a visible light source, and the light receiver can be a field aperture, etc. The light detector 5 uses a light scattering method for detection. The light scattering method is based on the principle of light scattering. When a light beam is incident on a particle, it will scatter around the space. The various scattering parameters of the light are closely related to the particle size, which provides a scale for the measurement of the particle.
[0052] A standard microbial suspension of known concentration is used for calibration to ensure the accuracy of the measurement results. The measured scattered light intensity is converted into microbial concentration based on the relationship between the scattered light intensity and the microbial concentration (a standard curve is established in advance through experiments).
[0053] Example 2
[0054] In another typical embodiment of the present invention, Figure 1-Figure 2 As shown, a test method for a microorganism concentration detection device is provided, using the microorganism concentration detection device as in Example 1.
[0055] A test method for a microbial concentration detection device, comprising:
[0056] Take the sealed culture bottle and slide it into the guide slot along the open section until the culture bottle abuts against the blocking end 4 and stabilizes. The culture bottle is tilted so that the culture liquid level in the culture bottle is higher than the light detector 5.
[0057] The light source of the light detector 5 outputs the detection light to enter the culture bottle vertically or obliquely relative to the side wall of the culture bottle; the light receiver collects the detection light reflected from the culture liquid and converts it into an electrical signal;
[0058] The controller acquires and processes the electrical signal and calculates the microbial concentration of the culture solution in the culture bottle.
[0059] The culture bottle forms surface contact or point contact with the positioning surface 7, so that when different culture bottles are detected, the distance between the incident point of the light source and the detection light on the side wall of the culture bottle is equal.
[0060] After the culture bottle reaches a stable posture in the positioning slide groove 2, the wall of the culture bottle facing the light source approaches a vertical state, and the light emitted by the light source is transmitted into the culture bottle nearly perpendicular to the container wall. When the incidence is vertical, the light path is relatively short and direct, and the background noise (such as scattered light from the container wall) is relatively small, making the detected scattered light more likely to come from microbial particles, thereby improving the accuracy of the test.
[0061] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A microorganism concentration detection device, characterized in that: include: A base is provided with an inclined guide slot, the lower end of the guide slot relative to the bottom surface of the base is a blocked end, and the other end is an open end, the blocked end forms a positioning surface abutting the side wall of the culture bottle, and the bottom surface of the guide slot serves as a bearing surface contacting the bottom wall of the culture bottle; a light detector includes a light source and a light receiver facing the positioning slot, with a perpendicular bisector of a line connecting the guide side surfaces on both sides of the guide slot as a reference plane, and the distribution positions of the light source and the light receiver are coplanar with the reference plane.
2. The microorganism concentration detection device according to claim 1, characterized in that: The positioning surface is a curved surface, and two ends of the curved surface are respectively connected to the guide side surfaces.
3. The microorganism concentration detection device according to claim 2, characterized in that: The trajectory of the positioning surface is in an arc shape, and the projection shape of the positioning surface with the bearing surface as the projection surface is in an arc shape.
4. The microorganism concentration detection device according to claim 1, characterized in that: The generatrix of the side wall of the culture bottle carried by the carrying surface is perpendicular to the reflected light, so that the light is vertically or obliquely captured relative to the side wall of the culture bottle.
5. The microorganism concentration detection device according to claim 4, characterized in that: The contact position between the culture bottle and the positioning surface is symmetrical with respect to the reference surface, or the contact position is coplanar with the reference surface.
6. The microorganism concentration detection device according to claim 5, characterized in that: The culture bottle is in surface contact with the positioning surface, and the contact surface is a symmetrical structure relative to the reference surface, or the culture bottle is in point contact with the positioning surface, and the contact point is coplanar with the reference surface.
7. The microorganism concentration detection device according to claim 1, characterized in that: The light sources of the light detectors are located on the same side of the guide slots.
8. The microorganism concentration detection device according to claim 7, characterized in that: The light detector is connected to the controller, the light detector is used to emit detection light and receive reflected light, and the controller is used to control the light detector to emit detection light and obtain reflected light information received by the light detector.
9. A test method for a microorganism concentration detection device, using the microorganism concentration detection device as claimed in any one of claims 1 to 8, characterized in that: include: Take the sealed culture bottle and slide it into the guide groove along the open section until the culture bottle abuts against the sealed end and stabilizes. The culture bottle is tilted so that the culture liquid level in the culture bottle is higher than the light detector. The light source of the light detector outputs the detection light to enter the culture bottle vertically or obliquely relative to the side wall of the culture bottle; the light receiver collects the detection light reflected from the culture liquid and converts it into an electrical signal; The controller acquires and processes the electrical signal and calculates the microbial concentration of the culture solution in the culture bottle.
10. The test method of the microorganism concentration detection device according to claim 9, characterized in that: The culture bottle forms surface contact or point contact with the positioning surface, so that when different culture bottles are detected, the distance between the incident point of the light source and the detection light on the side wall of the culture bottle is equal.