Liquid chromatography-tandem mass spectrometry detection system and waste gas emission method

Abstract

The embodiment of the invention provides a liquid chromatography-tandem mass spectrometry detection system and a waste gas emission method. The system comprises a mass spectrometer, a liquid chromatograph and a controller. The mass spectrometer comprises an ion source and an exhaust emission device, and the ion source is communicated with the liquid chromatograph; the waste gas emission device comprises a first waste gas channel, a heat dissipation assembly, a second waste gas channel and a turbine assembly. Waste gas discharged by the ion source enters an inlet of the first waste gas channel; an inlet of the heat dissipation assembly communicates with an outlet of the first waste gas channel and is used for cooling waste gas. An inlet of the second waste gas channel communicates with an outlet of the heat dissipation assembly. The turbine assembly is arranged at the outlet of the second waste gas channel so as to control the cooled waste gas to be discharged through the outlet of the second waste gas channel. The controller acquires a liquid phase parameter of the chromatograph and an ion source parameter of the ion source to determine an exhaust emission speed of the mass spectrometer and a target rotational speed of the turbine assembly. And the turbine assembly is controlled to be matched with different rotating speeds under different detection objects, so that the sensitivity of the mass spectrometer is greatly improved.

Description

Technical Field

[0001] The present invention relates to the technical field of mass spectrometry equipment, and in particular to a liquid chromatography tandem mass spectrometry detection system and an exhaust gas emission method. Background Art

[0002] Mass spectrometer is an instrument used to separate and detect different inorganic or organic compounds. Liquid chromatography tandem mass spectrometry (LC-MS / MS) detection system combines the high separation capability of liquid chromatography with the high sensitivity and high selectivity detection capability of tandem mass spectrometer. It has been widely used in many fields such as life sciences, pharmaceutical research and development, food safety, and environmental monitoring.

[0003] During the use of liquid chromatography tandem mass spectrometer, the ion source will produce a large amount of waste solvent and sample during the ionization process. These substances will flow back in the closed ion source cavity, causing memory effect and contamination of the ion source, thereby affecting the sensitivity of the instrument. Therefore, there is an urgent need for a device and method for flexibly discharging the waste gas of the mass spectrometer in the liquid chromatography tandem mass spectrometry detection system. Summary of the invention

[0004] The present application discloses a liquid chromatography tandem mass spectrometry detection system and an exhaust gas emission method, which are intended to solve the problem that a large amount of waste solvents and samples are generated during the ionization process of the current ion source, and these substances reflux in the closed ion source cavity, causing memory effect and contamination of the ion source, thereby affecting the sensitivity of the instrument.

[0005] First invention, the present application provides a liquid chromatography tandem mass spectrometry detection system, the liquid chromatography tandem mass spectrometry detection system comprising:

[0006] Liquid chromatograph, which separates the compounds to be detected;

[0007] A mass spectrometer, the mass spectrometer includes an ion source and an exhaust gas discharge device, the inlet of the ion source is connected to the liquid chromatograph, the separated compounds enter the ion source for ionization and generate exhaust gas, and the exhaust gas discharge device includes a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel and a turbine component; the first exhaust gas channel has an inlet and an outlet, and the exhaust gas enters the inlet of the first exhaust gas channel; the inlet of the heat dissipation component is connected to the outlet of the first exhaust gas channel, and the heat dissipation component is used to cool the exhaust gas; the inlet of the second exhaust gas channel is connected to the outlet of the heat dissipation component; the turbine component is arranged at the outlet of the second exhaust gas channel, and is used to control the cooled exhaust gas to be discharged through the outlet of the second exhaust gas channel;

[0008] The controller is electrically connected to the liquid chromatograph and the mass spectrometer respectively, and is used to obtain the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source, confirm the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, confirm the target speed corresponding to the turbine assembly according to the exhaust gas emission speed, and control the turbine assembly to operate at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel.

[0009] In some embodiments, the heat dissipation component includes: an exhaust box, wherein a plurality of heat dissipation pipes are arranged in the exhaust box, the inlet of the exhaust box is connected to the outlet of the first exhaust channel, the outlet of the exhaust box is connected to the inlet of the second exhaust channel, and the extension direction of the heat dissipation pipe is perpendicular to the flow direction of the exhaust gas; an air drive member, wherein the air drive member is arranged on one side of the exhaust box, and the air drive member is used to suck in cold air from the other side of the heat dissipation pipe, so that the exhaust gas is cooled after contacting the pipe wall of the heat dissipation pipe in the exhaust box; a mounting plate, wherein the mounting plate is used to fix the air drive member on the exhaust box, and the mounting plate includes a plurality of air holes, and the air holes are arranged corresponding to the heat dissipation pipe; wherein the controller confirms the exhaust gas cooling speed according to the exhaust gas discharge speed, and determines the air drive power of the air drive member according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes, so as to control the operation of the air drive member according to the air drive power.

[0010] Exemplarily, the flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe; and / or the inlet diameter of the second exhaust gas channel is much larger than the outlet diameter of the second exhaust gas channel.

[0011] In some embodiments, the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph.

[0012] In some embodiments, the ion source parameters of the ion source include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source.

[0013] In some embodiments, the liquid chromatography-tandem mass spectrometry detection system also includes: a mass analysis detection device, which is connected to the ion source, and the ion source inputs the ions after ionization of the compound into the mass analysis detection device for detection; the controller obtains reserpine spectrum information and / or triiodothyronine spectrum information corresponding to the ions to update the target speed of the turbine assembly according to the reserpine spectrum information and / or triiodothyronine spectrum information.

[0014] In a second aspect, the present application provides an exhaust gas emission method, which is applied to a controller of a liquid chromatography tandem mass spectrometry detection system provided in any embodiment of the present application, and the method comprises:

[0015] Acquire the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph; the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source;

[0016] Determine the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, and determine the target rotation speed corresponding to the turbine component of the exhaust gas emission device according to the exhaust gas emission speed;

[0017] The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas passage of the exhaust gas discharge device.

[0018] In some embodiments, the method also includes: obtaining reserpine spectrum information of ions detected by the mass analysis detection device; confirming a first peak area corresponding to the mass spectrometer based on the reserpine spectrum information; obtaining a first difference based on the first peak area and the first target peak area, so as to update the target speed based on the first difference; and / or, obtaining triiodothyronine spectrum information of ions detected by the mass analysis detection device; confirming a second peak area corresponding to the mass spectrometer based on the triiodothyronine spectrum information; obtaining a second difference based on the second peak area and the second target peak area, so as to update the target speed based on the second difference.

[0019] In some embodiments, before the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel of the exhaust gas emission device, it also includes: confirming the exhaust gas cooling speed according to the exhaust gas emission speed; determining the wind driving power of the wind driving part of the heat dissipation component according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes of the heat dissipation component; and controlling the operation of the wind driving part of the heat dissipation component according to the wind driving power.

[0020] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, it also includes: obtaining exhaust gas temperature information at the outlet of the second exhaust gas channel; and adjusting the power of the wind driving component of the heat dissipation component according to the exhaust gas temperature information.

[0021] In a third aspect, the present application provides a controller comprising a processor, a memory, and a computer program stored on the memory and executable by the processor, wherein the memory stores a strategy model, wherein when the computer program is executed by the processor, an exhaust gas emission method as provided in any embodiment of the present application is implemented.

[0022] In a fourth aspect, the present application provides a computer-readable storage medium having a computer program stored thereon, and when the computer program is executed by a processor, the processor implements the exhaust gas emission method provided in any embodiment of the present application.

[0023] The embodiment of the present application provides a liquid chromatography tandem mass spectrometry detection system and an exhaust gas emission method, and the provided system includes: a mass spectrometer, a liquid chromatograph and a controller. The mass spectrometer includes an ion source and an exhaust gas emission device, the liquid chromatograph separates the compounds to be detected, the ion source is connected to the liquid chromatograph, and the separated compounds enter the ion source for ionization and generate exhaust gas; the exhaust gas emission device includes a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel and a turbine component; the first exhaust gas channel has an inlet and an outlet, and the exhaust gas emitted by the ion source enters the inlet of the first exhaust gas channel; the inlet of the heat dissipation component is connected to the outlet of the first exhaust gas channel, and the heat dissipation component is used to cool the exhaust gas; the inlet of the second exhaust gas channel is connected to the outlet of the heat dissipation component; the turbine component is arranged at the outlet of the second exhaust gas channel, and is used to control the exhaust gas after cooling to be discharged through the outlet of the second exhaust gas channel.

[0024] The controller is electrically connected to the liquid chromatograph and the mass spectrometer respectively. The provided method obtains the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source through the controller, confirms the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, confirms the target speed corresponding to the turbine assembly according to the exhaust gas emission speed, and controls the turbine assembly to work at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel. Then, by introducing the exhaust gas emission speed, the target speed corresponding to the turbine assembly is optimized, so as to achieve the effect of improving the sensitivity of mass spectrometry detection, and solve the defect of the prior art that the detection sensitivity cannot be optimized by adjusting the exhaust gas emission speed. And different detection objects are matched with different turbine assembly speeds, so that the sensitivity of the mass spectrometer in the liquid chromatography tandem mass spectrometry detection system is greatly improved.

[0025] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 It is a schematic block diagram of the structure of a liquid chromatography tandem mass spectrometry detection system provided in one embodiment of the present application;

[0028] Figure 2 It is a schematic structural diagram of an exhaust gas emission device provided in one embodiment of the present application;

[0029] Figure 3 is a schematic flow chart of the steps of an exhaust gas emission method provided in one embodiment of the present application;

[0030] Figure 4 is a schematic diagram of reserpine spectrum information provided in one embodiment of the present application;

[0031] Figure 5 is a schematic diagram of triiodothyronine spectrum information provided in an embodiment of the present application;

[0032] Figure 6 It is a schematic block diagram of the structure of a controller provided in one embodiment of the present application.

[0033] It should be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present application. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0035] The flowcharts shown in the accompanying drawings are only examples and do not necessarily include all the contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may also be decomposed, combined or partially merged, so the actual execution order may change according to actual conditions.

[0036] It should be understood that the terms used in this application specification are only for the purpose of describing specific embodiments and are not intended to limit the application. As used in this application specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0037] It should be understood that, in order to clearly describe the technical solutions of the embodiments of the present application, in the embodiments of the present application, words such as "first" and "second" are used to distinguish the same items or similar items with substantially the same functions and effects. Those skilled in the art can understand that words such as "first" and "second" do not limit the quantity and execution order, and words such as "first" and "second" do not necessarily limit the difference.

[0038] It should be further understood that the term “and / or” used in the specification and appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0039] To facilitate understanding of the embodiments of the present application, some words involved in the embodiments of the present application are briefly explained below.

[0040] 1. Liquid chromatography tandem mass spectrometry detection system: Detecting various indicators of patient blood, body fluids and other samples collected by clinical laboratories can provide important basis for disease prevention, diagnosis, treatment plans and prognosis. Traditional detection methods such as immunoassay (IA) based on antigen-antibody binding are one of the routine clinical detection methods, but clinical practice has found that the IA method will have poor selectivity or false positive results. Liquid chromatography-tandem mass spectrometry (LC-MS / MS) technology, with its advantages of high sensitivity and high selectivity, can provide more accurate analysis results and plays an important role in clinical testing.

[0041] The ionization mode of mass spectrometry can generally be divided into hard ionization and soft ionization. The hard ionization mode is currently limited to the analysis of volatile and thermally stable compounds and is not suitable for the analysis of clinical samples. Soft ionization allows sample molecules to maintain their intact form during the ionization process, which is conducive to the detection of clinical small molecule analytes. Electrospray ionization is the most common ionization mode of clinical mass spectrometry. After the intact sample molecules enter the tandem mass spectrometer, the parent ions of specific compounds are selected in the primary mass spectrometer, and the parent ions are collided and fragmented in the collision cell. Subsequently, the MRM or SRM mode of the secondary mass spectrometer is used to obtain the corresponding ion pair information. Compared with other methods such as ultraviolet spectrophotometry, fluorescence spectroscopy and immunoassay, tandem mass spectrometry has the advantages of higher selectivity, accuracy, resolution, sensitivity, etc., and can achieve accurate quantification of multiple analytes simultaneously through the MRM / SRM mode.

[0042] LC-MS / MS based on liquid chromatography separation is currently the most commonly used detection method in clinical laboratories. Since clinical test samples are usually biological liquids or solids with complex matrices, they are not suitable for direct mass spectrometry analysis, and the samples must be pretreated and chromatographically separated. Among them, sample preparation during the pretreatment process will not only affect the chromatographic separation effect, but also affect the analyte signal intensity. Sometimes it is necessary to concentrate or dilute the sample according to the detection limit of the LC-MS / MS system used, and separate the target from the interfering components by optimizing the liquid chromatography conditions.

[0043] 2. Ion source: As the core of the mass spectrometer, the ion source can ionize the organic solution to form gaseous ions and output the gaseous ions to the mass analyzer so that the mass analyzer can analyze and detect the gaseous ions.

[0044] Taking the electrospray ion source as an example, after the organic solution is introduced into the electrospray ion source, it can be ionized to form an ionic liquid under the action of high voltage electricity or high voltage electric field. After the ionic liquid is atomized by high pressure, the solvent can be evaporated in the high temperature gas to form gaseous ions. However, most of the gaseous ions in the ion source are sucked away by the air pump under the guidance of gravity and airflow, and only a very small number of gaseous ions can be output to the outside of the ion source and transmitted to the mass analyzer, which also leads to a limited collision rate of ions in the mass spectrometer and poor sensitivity of the mass spectrometer.

[0045] In conjunction with the accompanying drawings, some embodiments of the present application are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0046] Liquid chromatography tandem mass spectrometry (LC-MS / MS) combines the high separation capability of liquid chromatography with the high sensitivity and high selectivity of tandem mass spectrometry. Since its introduction, this technology has been widely used in many fields such as life sciences, pharmaceutical research and development, food safety, and environmental monitoring due to its unique advantages.

[0047] During the use of LC-MS / MS, the sample solution is first separated by liquid chromatography and then enters the ion source for ionization. The charged ions enter the mass spectrometer for detection under the action of potential difference and vacuum difference. The ion source will produce a large amount of waste solvent and sample during the ionization process. These substances reflux in the closed ion source cavity, causing memory effect and contamination of the ion source, thereby affecting the sensitivity of the instrument. Therefore, the exhaust gas from the ion source needs to be discharged and treated.

[0048] Most of the existing ion source exhaust gas discharge uses a mechanical vacuum pump as the exhaust gas exhaust device. The exhaust structure has a single function and cannot adjust the speed. If the exhaust gas discharge speed is too high, some target analytes will be discharged and cannot enter the mass spectrometer, resulting in a lower detection signal; if the exhaust gas discharge speed is too low, the exhaust gas cannot be effectively discharged, resulting in instrument contamination and reduced instrument sensitivity. At present, there is no application in the mass spectrometry detection process to achieve the effect of improving sensitivity by adjusting the exhaust gas discharge speed.

[0049] Therefore, based on the above content, in order to solve the problem that the waste solvent and sample reflux in the ion source affect the sensitivity of the mass spectrometer. Figure 1 The liquid chromatography tandem mass spectrometry detection system 100 is shown. Figure 1 As shown, the liquid chromatography tandem mass spectrometry detection system 100 includes a mass spectrometer 10, a controller 20 and a liquid chromatograph 30. The liquid chromatograph 30 is used to separate compounds to be detected (such as organic compounds). The mass spectrometer 10 includes an exhaust gas discharge device 11 and an ion source 12. The ion source 12 is connected to the liquid chromatograph 30, and the separated compounds enter the ion source 12 for ionization and generate exhaust gas.

[0050] like Figure 2 As shown, the exhaust gas discharge device 11 includes a first exhaust gas channel 111, a heat dissipation component 112, a second exhaust gas channel 113 and a turbine component 114; the first exhaust gas channel 111 has an inlet and an outlet, and the exhaust gas discharged by the ion source 20 enters the inlet of the first exhaust gas channel 111; the inlet of the heat dissipation component 112 is connected to the outlet of the first exhaust gas channel 111, and the heat dissipation component 112 is used to cool the exhaust gas; the inlet of the second exhaust gas channel 113 is connected to the outlet of the heat dissipation component 112; the turbine component 114 is arranged at the outlet of the second exhaust gas channel 113, and is used to control the cooled exhaust gas (or the liquid formed after the exhaust gas is condensed) to be discharged through the outlet of the second exhaust gas channel 113.

[0051] The controller 20 is electrically connected to the liquid chromatograph 30 and the mass spectrometer 10, respectively, and is used to obtain the liquid phase parameters of the liquid chromatograph 30 and the ion source parameters of the ion source 12, confirm the exhaust gas emission speed corresponding to the mass spectrometer 10 according to the liquid phase parameters and the ion source parameters, confirm the target speed corresponding to the turbine assembly 114 according to the exhaust gas emission speed, and control the turbine assembly 114 to work at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel.

[0052] Furthermore, the provided method can adjust the rotation speed of the turbine assembly 114 so that the mass spectrometer can accurately and efficiently discharge the waste gas generated by the ion source 12. This improves the detection efficiency and sensitivity of the mass spectrometer 10. Different detection objects can be matched with different rotation speeds of the turbine assembly 114, which greatly improves the sensitivity of the mass spectrometer.

[0053] It should be noted that the present application can discharge hot exhaust gas generated by various types of mass spectrometers 10 , and the present application takes a liquid chromatography tandem mass spectrometer as an example for explanation.

[0054] In some embodiments, Figure 2 As shown, the heat dissipation assembly 112 includes: an exhaust box 1121, a heat dissipation pipe 1122, a wind-driving member 1123 and a mounting plate 1124. A plurality of heat dissipation pipes 1122 are arranged in the exhaust box 1121, the inlet of the exhaust box 1121 is connected to the outlet of the first exhaust channel 111, the outlet of the exhaust box 1121 is connected to the inlet of the second exhaust channel 113, and the extension direction of the heat dissipation pipe 1122 is perpendicular to the flow direction of the exhaust gas. The wind-driving member 1123 is arranged on one side of the exhaust box 1121, and the wind-driving member 1123 is used to inhale cold air from the other side of the heat dissipation pipe 1122, so that the exhaust gas is cooled after contacting the pipe wall of the heat dissipation pipe 1122 in the exhaust box 1121. The mounting plate 1124 is used to fix the wind-driving member 1123 on the exhaust box 1121, and the mounting plate 1124 includes a plurality of vents, which are arranged corresponding to the heat dissipation pipe 1122.

[0055] The controller 20 determines the exhaust gas cooling speed according to the exhaust gas emission speed, determines the wind driving power of the wind driving member 1123 according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes, and controls the operation of the wind driving member 1123 according to the wind driving power. The provided method can control the power of the wind driving member 1123 in coordination with the exhaust gas emission speed while controlling the turbine assembly 114, thereby improving the efficiency of exhaust gas emission.

[0056] For example, Figure 2 As shown, the wind driving member 1123 is a heat dissipation fan, which can be installed on any opening side of the heat dissipation pipe 1122 and can suck in the cold air on the other side to achieve a cooling effect.

[0057] Exemplarily, the heat dissipation pipe 1122 is made of a heat-conducting material, such as aluminum or copper, so that the exhaust gas can contact the side wall of the heat dissipation pipe 1122 to be cooled.

[0058] For example, the flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe. This can prevent the turbine assembly 114 from contacting the heat dissipation assembly 112 (e.g. Figure 2 The turbine assembly 114 and the wind driving member 12 will not be in the same direction).

[0059] For example, the inlet diameter of the second exhaust passage is much larger than the outlet diameter of the second exhaust passage, thereby improving the exhaust gas emission efficiency of the turbine assembly 114 .

[0060] In some embodiments, the turbine assembly 114 is a turbo blower, and the exhaust gas emission rate of the mass spectrometer 10 can be controlled by controlling the rotation speed of the turbo blower.

[0061] In some embodiments, the liquid phase parameters of the liquid chromatograph 30 include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph 30. The mobile phase composition is as follows: mobile phase A: 0.1% formic acid water, mobile phase B: 0.1% formic acid acetonitrile, column temperature is as follows: the temperature of the chromatographic column, such as using a C18 column (2.1*30mm, 1.3μm) to obtain its corresponding column temperature. At the same time, the liquid phase parameters may also include, for example, the injection volume. Then, the corresponding exhaust gas emission rate can be determined in combination with the current liquid phase parameters.

[0062] In some embodiments, the ion source parameters of the ion source 12 include at least one or more of the ion source voltage, the nebulizer gas pressure, the auxiliary heating gas pressure, and the heating rod temperature of the ion source 12. The ion source parameters may also be referred to as mass spectrometry conditions, and may also include parameters such as the entrance voltage, the collision voltage, and the potential difference to improve the optimization efficiency of the exhaust gas emission velocity and the target rotation speed of the turbine assembly 114 in combination with the ion source information of the mass spectrometer.

[0063] In some embodiments, the provided liquid chromatography tandem mass spectrometry detection system 100 also includes: a mass analysis detection device (such as consisting of a mass analyzer and a mass detector), the mass analysis detection device is connected to the ion source 12, and the ion source 12 inputs the ions after ionization of the compound into the mass analysis detection device for detection; the controller 20 obtains the reserpine spectrum information and / or triiodothyronine (T3) spectrum information corresponding to the ions to update the target speed of the turbine assembly according to the reserpine spectrum information and / or triiodothyronine spectrum information.

[0064] In some embodiments, the controller 10 may be a microcontroller unit (MCU), or an external terminal device such as a computer device, a server, a tablet, etc. The embodiment of the present application does not limit the type of the controller 10 .

[0065] The embodiment of the present application provides a liquid chromatography tandem mass spectrometry detection system, which optimizes the target speed corresponding to the turbine assembly by introducing the exhaust gas emission speed of the mass spectrometer in the system, thereby improving the sensitivity of mass spectrometry detection, and solving the defect of the prior art that the detection sensitivity cannot be optimized by adjusting the exhaust gas emission speed. Different detection objects are matched with different turbine assembly speeds, so that the sensitivity of the mass spectrometer in the system is greatly improved.

[0066] See also Figure 3 The embodiment of the present application provides a method for exhaust gas emission, and the provided method is applied to the controller of the liquid chromatography tandem mass spectrometry detection system provided in any embodiment of the present application. Figure 3 As shown, the provided method includes steps S201 to S203.

[0067] S201. Obtain the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph; the ion source parameters include at least one or more of the ion source voltage, nebulizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source.

[0068] S202. Confirm the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, and confirm the target rotation speed corresponding to the turbine component of the exhaust gas emission device according to the exhaust gas emission speed.

[0069] S203. Control the turbine device to operate according to the target speed, and discharge the cooled exhaust gas through the outlet of the second exhaust gas channel of the exhaust gas emission device.

[0070] Then, the controller can obtain the liquid phase parameters of the mass spectrometer and the ion source parameters of the ion source, and can confirm the corresponding exhaust gas emission speed through a preset neural network model (such as a convolutional neural network model), or confirm the corresponding exhaust gas emission speed in the mapping relationship between the preset liquid phase parameters and the ion source parameters and the exhaust gas emission speed. Then, the target speed corresponding to the turbine assembly is confirmed according to the exhaust gas emission speed to improve the sensitivity of the mass spectrometer.

[0071] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further includes: obtaining reserpine spectrum information of the ions detected by the mass analysis detection device; confirming the first peak area corresponding to the mass spectrometer according to the reserpine spectrum information; obtaining a first difference according to the first peak area and the first target peak area, so as to update the target speed according to the first difference. Then, the target speed of the turbine assembly can be optimized and updated according to the peak area corresponding to the reserpine spectrum information.

[0072] For example, without changing the liquid phase parameters and ion source parameters, when monitoring the quantitative ion of reserpine (609.3→195.1), when manually adjusting the speed of the turbine assembly, Figure 4 As shown ( Figure 4 (a) Figure 4 (b) Figure 4 (c) Figure 4 The blue curve in (d) represents the reserpine spectrum of the reserpine quantitative ion (609.3→195.1), and the remaining gray, red, and green colors represent the reserpine spectra of the remaining auxiliary ion pairs, respectively. Figure 4 (a) is the reserpine spectrum with the turbine assembly speed manually adjusted to 20460 r / min; Figure 4 (b) is the reserpine spectrum with the turbine assembly speed manually adjusted to 41400 r / min; Figure 4 (c) is the spectrum of reserpine when the turbine assembly speed is manually adjusted to 46020 r / min. Figure 4 In (a), (b), and (c), the uniform observation time is 2.35 s. Figure 4 (a) Figure 4 (b) and Figure 4 The peak areas of the blue curves in (c) are 16816.8, 22606.5, and 22318.8, respectively.

[0073] The target speed obtained by the provided method is 28860r / min after the target speed is optimized. Figure 4 (d) is the spectrum of reserpine when the turbine assembly speed is adjusted to 28860 r / min by the provided method. It can be seen that under the same conditions, the peak area of ​​the blue curve is 25214.7. Figure 4 The vertical coordinates of the reserpine spectra can be seen in Figure 4 The vertical coordinate of the blue curve in (d) is the strongest intensity value.

[0074] It can be seen that after optimization, when the target speed is 28860 r / min, the peak area of ​​the reserpine quantitative ion (609.3→195.1) is the highest, which is 25214.7, which is nearly 50% higher than the peak area (16816.8) at a speed of 20460 r / min. That is, the method provided in this application can quickly determine the target speed of the turbine assembly corresponding to the liquid phase parameters and the ion source parameters.

[0075] In some embodiments, triiodothyronine spectrum information of ions detected by a mass analysis detection device is obtained; a second peak area corresponding to the mass spectrometer is confirmed according to the triiodothyronine spectrum information; a second difference is obtained according to the second peak area and the second target peak area, so as to update the target speed according to the second difference. Then, the target speed of the turbine assembly can be optimized and updated according to the peak area corresponding to the triiodothyronine spectrum information.

[0076] For example, without changing the liquid phase parameters and the ion source parameters, when monitoring the triiodothyronine quantitative ion (652.0→605.8), when manually adjusting the speed of the turbine assembly, Figure 5 As shown ( Figure 5 (a) Figure 5 (b) Figure 5 (c) Figure 5 The blue curve in (d) represents the triiodothyronine spectrum of the triiodothyronine quantitative ion (652.0→605.8), and the remaining gray, red and green colors represent the triiodothyronine spectra of the remaining auxiliary ion pairs, respectively. Figure 5 (a) is the spectrum of triiodothyronine when the turbine assembly speed is manually adjusted to 16560 r / min; Figure 5 (b) is the triiodothyronine spectrum when the turbine assembly speed is manually adjusted to 25260 r / min; Figure 5 (c) is the spectrum of triiodothyronine when the turbine assembly speed is manually adjusted to 34860 ​​r / min. Figure 5 In (a), (b), and (c), the uniform observation time is 3.69 s. Figure 5 (a) Figure 5 (b) and Figure 5 The peak areas of the blue curve in (c) are 37464, 39282, and 46360, respectively (the blue curve represents the triiodothyronine quantitative ion (652.0→605.8), and the red curve represents the triiodothyronine spectrum of the auxiliary ion pair).

[0077] The target speed obtained by the provided method is 45660r / min after the target speed is optimized. Figure 5 (d) is the chromatographic information of triiodothyronine when the turbine assembly speed is adjusted to 45660 r / min by the provided method. It can be seen that under the same conditions, the peak area of ​​the blue curve is 51062. Figure 5 The vertical coordinates of the triiodothyronine spectra can be seen in Figure 5 The vertical coordinate of the blue curve in (d) is the strongest intensity value.

[0078] It can be seen that after optimization, under the condition of the target speed of 45660 r / min, the peak area of ​​the triiodothyronine quantitative ion (652.0→605.8) is the highest, which is 51062, compared with the peak area (37464) at the speed of 16560 r / min, which is 36.3% higher. That is, the method provided in this application can quickly determine the target speed of the turbine assembly corresponding to the liquid phase parameters and the ion source parameters to obtain the optimal results corresponding to each indicator.

[0079] In some embodiments, before the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel of the exhaust gas emission device, it also includes: confirming the exhaust gas cooling speed according to the exhaust gas emission speed; determining the wind driving power of the wind driving part of the heat dissipation component according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes of the heat dissipation component; and controlling the operation of the wind driving part of the heat dissipation component according to the wind driving power.

[0080] Furthermore, the provided method can quickly determine the wind driving power of the wind driving component (such as a cooling fan) required to achieve the expected exhaust gas cooling speed through the number and size of the heat pipes, ensuring that the wind driving component can meet the heat dissipation requirements of the mass spectrometer.

[0081] In some embodiments, after the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, it also includes: obtaining exhaust gas temperature information at the outlet of the second exhaust gas channel; and adjusting the power of the wind driving component of the heat dissipation assembly according to the exhaust gas temperature information.

[0082] When the second exhaust gas passage discharges the exhaust gas or the exhaust liquid formed by the condensation of the exhaust gas, the power of the wind driving member is adjusted if the temperature of the exhaust liquid is too high by detecting the temperature of the exhaust liquid.

[0083] The embodiment of the present application provides an exhaust gas emission method, which optimizes the target speed corresponding to the turbine assembly by introducing the exhaust gas emission speed of the mass spectrometer, thereby achieving the effect of improving the mass spectrometer detection sensitivity, solving the defect of the prior art that the detection sensitivity cannot be optimized by adjusting the exhaust gas emission speed. Different detection objects are matched with different turbine assembly speeds, so that the sensitivity of the mass spectrometer is greatly improved.

[0084] See also Figure 6 , Figure 6 is a schematic block diagram of a controller provided in an embodiment of the present application. The controller may be a server. Figure 6 The controller includes a processor, a memory, and a network interface connected via a system bus, wherein the memory may include a non-volatile storage medium and an internal memory.

[0085] The non-volatile storage medium can store an operating system and a computer program. The computer program includes program instructions, and when the program instructions are executed, the processor can execute any exhaust gas emission method.

[0086] The processor is used to provide computing and control capabilities and support the operation of the entire controller.

[0087] The internal memory provides an environment for the operation of the computer program in the non-volatile storage medium. When the computer program is executed by the processor, the processor can execute any exhaust emission method.

[0088] The network interface is used for network communication, such as sending assigned tasks, etc. Those skilled in the art will understand that Figure 6 The structure shown in the figure is merely a block diagram of a partial structure related to the scheme of the present application, and does not constitute a limitation on the controller to which the scheme of the present application is applied. The specific controller may include more or fewer components than shown in the figure, or combine certain components, or have a different arrangement of components.

[0089] It should be understood that the processor may be a central processing unit (CPU), and the processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. Among them, the general-purpose processor may be a microprocessor or the processor may also be any conventional processor, etc.

[0090] In one embodiment, the processor is used to run a computer program stored in the memory to implement the following steps:

[0091] Obtaining the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph; and the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source;

[0092] Confirm the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, and confirm the target speed corresponding to the turbine component of the exhaust gas emission equipment according to the exhaust gas emission speed;

[0093] The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas passage of the exhaust gas discharge device.

[0094] A computer-readable storage medium is also provided in an embodiment of the present application, wherein the computer-readable storage medium stores a computer program, wherein the computer program includes program instructions, and the processor executes the program instructions to implement any one of the exhaust gas emission methods provided in the embodiments of the present application.

[0095] The computer-readable storage medium may be an internal storage unit of the controller described in the foregoing embodiment, such as a hard disk or memory of the controller. The computer-readable storage medium may also be an external storage device of the controller, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (Secure Digital, SD) card, a flash card (Flash Card), etc., equipped on the controller.

[0096] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed in the present application, and these modifications or replacements should be included in the protection scope of the present application. Therefore, the protection scope of the present application shall be based on the protection scope of the claims.

Claims

1. A liquid chromatography tandem mass spectrometry detection system, It is characterized in that The liquid chromatography tandem mass spectrometry detection system comprises: A liquid chromatograph, wherein the liquid chromatograph separates the compounds to be detected; A mass spectrometer, the mass spectrometer comprising an ion source and an exhaust gas discharge device, the inlet of the ion source is communicated with a liquid chromatograph, the separated compounds enter the ion source for ionization and generate exhaust gas, the exhaust gas discharge device comprises a first exhaust gas channel, a heat dissipation component, a second exhaust gas channel and a turbine component; the first exhaust gas channel has an inlet and an outlet, the exhaust gas enters the inlet of the first exhaust gas channel; the inlet of the heat dissipation component is communicated with the outlet of the first exhaust gas channel, the heat dissipation component is used to cool the exhaust gas; the inlet of the second exhaust gas channel is communicated with the outlet of the heat dissipation component; the turbine component is arranged at the outlet of the second exhaust gas channel, and is used to control the exhaust gas after cooling to be discharged through the outlet of the second exhaust gas channel; A controller, wherein the controller is electrically connected to the liquid chromatograph and the mass spectrometer, respectively, and is used to obtain liquid phase parameters of the liquid chromatograph and ion source parameters of the ion source, confirm an exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, confirm a target speed corresponding to the turbine assembly according to the exhaust gas emission speed, and control the turbine assembly to operate at the target speed to discharge the cooled exhaust gas through the outlet of the second exhaust gas channel.

2. The liquid chromatography tandem mass spectrometry detection system according to claim 1, It is characterized in that The heat dissipation component comprises: An exhaust box, wherein a plurality of heat dissipation pipes are arranged in the exhaust box, an inlet of the exhaust box is connected to an outlet of the first exhaust passage, an outlet of the exhaust box is connected to an inlet of the second exhaust passage, and an extension direction of the heat dissipation pipe is perpendicular to a flow direction of the exhaust gas; An air-expelling member, the air-expelling member is arranged on one side of the exhaust box, and is used to draw cold air from the other side of the heat dissipation pipe, so that the exhaust gas is cooled after contacting the pipe wall of the heat dissipation pipe in the exhaust box; A mounting plate, the mounting plate is used to fix the wind drive member on the exhaust box, the mounting plate includes a plurality of vents, and the vents are arranged corresponding to the heat dissipation pipes; The controller confirms the exhaust gas cooling speed according to the exhaust gas emission speed, determines the wind driving power of the wind driving component according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes, and controls the operation of the wind driving component according to the wind driving power.

3. The liquid chromatography tandem mass spectrometry detection system according to claim 2, It is characterized in that The flow direction of the cooled exhaust gas in the second exhaust gas channel is perpendicular to the communication direction of the heat dissipation pipe; and / or, The inlet diameter of the second exhaust gas channel is much larger than the outlet diameter of the second exhaust gas channel.

4. The liquid chromatography tandem mass spectrometry detection system according to claim 1, It is characterized in that The liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph.

5. The liquid chromatography tandem mass spectrometry detection system according to claim 1, It is characterized in that The ion source parameters of the ion source include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source.

6. The liquid chromatography tandem mass spectrometry detection system according to claim 1, It is characterized in that The liquid chromatography tandem mass spectrometry detection system also includes: A mass analysis detection device, the mass analysis detection device is connected to the ion source, and the ion source inputs the ions after ionization of the compound into the mass analysis detection device for detection; The controller obtains reserpine spectrum information and / or triiodothyronine spectrum information corresponding to the ions to update the target rotation speed of the turbine assembly according to the reserpine spectrum information and / or triiodothyronine spectrum information.

7. A method for exhaust gas emission, It is characterized in that A controller for a liquid chromatography tandem mass spectrometry detection system according to any one of claims 1 to 6, the method comprising: Acquire the liquid phase parameters of the liquid chromatograph and the ion source parameters of the ion source; wherein the liquid phase parameters include at least one or more of the mobile phase composition, flow rate, column temperature and gradient of the liquid chromatograph; the ion source parameters include at least one or more of the ion source voltage, atomizing gas pressure, auxiliary heating gas pressure and heating rod temperature of the ion source; Determine the exhaust gas emission speed corresponding to the mass spectrometer according to the liquid phase parameters and the ion source parameters, and determine the target rotation speed corresponding to the turbine component of the exhaust gas emission device according to the exhaust gas emission speed; The turbine device is controlled to operate according to the target speed, and the cooled exhaust gas is discharged through the outlet of the second exhaust gas passage of the exhaust gas discharge device.

8. The method according to claim 7, It is characterized in that The method further comprises: Obtaining reserpine spectrum information of ions detected by a mass analysis detection device; confirming the first peak area corresponding to the mass spectrometer according to the reserpine spectrum information; obtaining a first difference value according to the first peak area and a first target peak area, so as to update the target rotation speed according to the first difference value; and / or, Acquiring triiodothyronine spectrum information of ions detected by a mass analysis detection device; Confirming the second peak area corresponding to the mass spectrometer according to the triiodothyronine spectrum information; A second difference is obtained according to the second peak area and the second target peak area, so as to update the target rotation speed according to the second difference.

9. The method according to claim 7, It is characterized in that Before discharging the cooled exhaust gas through the outlet of the second exhaust gas passage of the exhaust gas discharge device, the method further includes: determining the exhaust gas cooling rate according to the exhaust gas emission rate; Determine the wind driving power of the wind driving member of the heat dissipation assembly according to the exhaust gas cooling speed and the number and size of the heat dissipation pipes of the heat dissipation assembly; The operation of the wind driving component of the heat dissipation component is controlled according to the wind driving power.

10. The method according to claim 7, It is characterized in that After the cooled exhaust gas is discharged through the outlet of the second exhaust gas channel, the method further comprises: acquiring exhaust gas temperature information at an outlet of the second exhaust gas passage; The power of the wind driving member of the heat dissipation assembly is adjusted according to the exhaust gas temperature information.

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