Method for rapidly detecting six lignan components in schisandra chinensis based on LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry)

Abstract

The invention discloses a method for rapidly detecting six lignan components in schisandra chinensis based on LC-MS / MS. The method comprises the following steps: S1, preparing a standard solution containing schizandrin A, schisandrin B, schisandrin C, schizandrin A, schizandrin B and schisantherin A by adopting a methanol-formic acid aqueous solution; s2, drying the schisandra chinensis sample, crushing the schisandra chinensis sample in liquid nitrogen in a dark place, and adding a methanol-formic acid aqueous solution for ultrasonic extraction to obtain an extracting solution; the solid-liquid ratio of the schisandra chinensis powder to the methanol-formic acid aqueous solution is 1: 15; freezing the extracting solution, centrifuging, and putting supernate into a sample injection bottle to obtain a test solution; s3, detecting the standard solution by adopting LC-MS / MS to obtain a standard curve, and detecting the test solution under the same condition; and calculating the contents of the six lignan components in the test solution according to the standard curve. The method has the advantages of high sensitivity, good stability, rapidness, low cost, low detection limit and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of Schisandra chinensis detection, and more specifically, to a method for rapidly detecting six lignan components in Schisandra chinensis based on LC-MS / MS. Background Art

[0002] Schisandra chinensis is the dried mature fruit of Schisandra chinensis (Turcz.) Baill., a plant of the Magnoliaceae family. It is commonly known as "Northern Schisandra chinensis" and is mainly distributed in Jilin, Liaoning, Heilongjiang and other provinces in my country. Schisandra chinensis has the effects of astringency, invigorating qi and promoting body fluid, tonifying the kidney and calming the mind, as well as protecting the liver, sedative hypnosis, and regulating the cardiovascular system. Schisandra chinensis is rich in lignans. Among them, Schizandrin A (SA), Schizandrin B (SB), Schisandrol A (SLA), Schisandrol B (SLB), Schisantherin A (SNA) have the function of regulating the central nervous system, and Schisandrin B, Schisandrin B, Schisandrin C (SC) and Schisandrin A have the function of protecting liver cells.

[0003] At present, the standard for measuring Schisandrae Chinensis is to determine the content of schisandrin A. However, due to the diversity and complexity of the effective ingredients of traditional Chinese medicine, the determination of the content of a single ingredient is not enough to explain the quality of traditional Chinese medicine and cannot fully reflect the quality of Schisandrae Chinensis. Therefore, it is more in line with the characteristics of multi-components of traditional Chinese medicine to evaluate Schisandrae Chinensis with multiple indicators. However, there is no report on the simultaneous quantitative detection of schisandrin A, schisandrin B, schisandrin C, schisandrin A, schisandrin B, and schisandrin ester A in Schisandrae Chinensis. Summary of the invention

[0004] The purpose of the present invention is to overcome the above-mentioned defects of the prior art and provide a simple, highly sensitive and high-throughput method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS, so as to achieve simultaneous quantitative detection of six lignan components in Schisandra chinensis.

[0005] To achieve the above object, the technical solution of the present invention is as follows:

[0006] A method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS, wherein the lignan components include schisandrin A, schisandrin B, schisandrin C, schisandrin A, schisandrin B and schisandrin ester A;

[0007] The method comprises the following steps:

[0008] S1. Prepare standard solution:

[0009] A standard solution containing schisandrin A, schisandrin B, schisandrin C, schisandrin alcohol A, schisandrin alcohol B and schisandrin ester A was prepared by using methanol-formic acid aqueous solution;

[0010] S2. Prepare the test solution:

[0011] After the Schisandra chinensis sample was dried, it was crushed in liquid nitrogen in the dark to obtain Schisandra chinensis powder;

[0012] Adding methanol-formic acid aqueous solution to the Schisandra powder for ultrasonic extraction to obtain an extract; the solid-liquid ratio of the Schisandra powder to the methanol-formic acid aqueous solution is 1:15;

[0013] The extract is frozen, centrifuged, and the supernatant is placed in a sample injection bottle for analysis;

[0014] The volume percentage of formic acid in the methanol-formic acid aqueous solution in S1 and S2 is 0.1%, and the volume percentage of methanol is 80%;

[0015] S3, LC-MS / MS detection:

[0016] The standard solution is detected by LC-MS / MS to obtain a standard curve, and the test solution is detected under the same conditions;

[0017] Calculate the contents of the six lignan components in the test solution according to the standard curve;

[0018] The chromatographic detection conditions of LC-MS / MS described in S3 are as follows: chromatographic column: ZORBAX RRHD Eclipse Plus-C18 chromatographic column, specification is 2.1mmx50mm, 1.8μm; mobile phase: A: aqueous solution containing 0.1% formic acid, B: acetonitrile solution containing 0.1% formic acid; gradient elution program: 0.0min, 45%B; 0.0~4.0min: 95%B; 4.0~5.0min: 95%B; 5.0~5.5min: 45%B; flow rate: 0.3mL / min; injection volume: 1.0μL; column temperature: 35℃; injection plate temperature 10℃;

[0019] The mass spectrometry detection conditions of LC-MS / MS described in S3 are as follows: electrospray ion source; positive ion scanning mode; multiple reaction detection mode MRM; sheath gas, drying gas, and collision gas are all high-purity nitrogen; drying gas temperature: 350°C; drying gas flow rate: 7L / min; sheath gas temperature: 350°C; sheath gas flow rate: 12L / min; capillary voltage: 3500v; polarity: Positive; dwell time: 20ms; The mass spectrometry parameters of the six lignan components are as follows:

[0020]

[0021] Implementing the embodiments of the present invention will have the following beneficial effects:

[0022] (1) The present invention first optimizes the extraction method, and uses a methanol-formic acid aqueous solution to quickly and efficiently extract the target components (SA, SB, SC, SLA, SLB, SNA) in the Schisandra chinensis sample to be tested, and combines freezing and centrifugation to effectively remove the precipitated protein and chlorophyll in the sample. The extraction method is simple and reliable, with high extraction efficiency, and effectively reduces the interference of the sample matrix and impurity components on the LC-MS / MS measurement process, so that the extracted sample can meet the requirements of subsequent analysis, and the extraction agent used is cheap and low-toxic.

[0023] (2) The present invention is the first to establish a LC-MS / MS method for simultaneously determining the six lignan components (SA, SB, SC, SLA, SLB, and SNA) in Schisandra chinensis. The LC-MS / MS method embodies the complementary advantages of chromatography and mass spectrometry, and by optimizing the detection parameters of chromatography and mass spectrometry, a rapid and accurate quantitative analysis of the six main biphenylcyclooctene-type lignan components in Schisandra chinensis is achieved. The detection method of the present invention has the advantages of high sensitivity, good stability, accurate quantification, short detection time, low cost, low detection limit, and wide test range, which fills the gap in the current detection methods for the six substances SA, SB, SC, SLA, SLB, and SNA, and provides a reliable high-throughput detection method for the rapid, efficient, and accurate determination of the contents of the six components in different plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is the chemical structural formula of SA, SB, SC, SLA, SLB and SNA.

[0025] Figure 2 The chromatogram and mass spectrum of the six lignan components in Schisandra chinensis collected based on LC-MS / MS in the present invention. DETAILED DESCRIPTION

[0026] The present invention is further described below in conjunction with specific embodiments, but the present invention is not limited in any way.

[0027] The inventors have found that compared with traditional high performance liquid chromatography, liquid chromatography-tandem mass spectrometry (LC-MS / MS) has the characteristics of high selectivity and high sensitivity, and the sample does not need to be subjected to complicated pretreatment, and can perform more accurate qualitative and quantitative analysis on chemical components with low content and difficult to separate in traditional Chinese medicine compound. Based on this, the present invention provides a method for rapid detection of 6 lignan components in Schisandra chinensis based on LC-MS / MS, comprising the following steps:

[0028] S1. Preparation of standard solution: A standard solution containing schisandrin A, schisandrin B, schisandrin C, schisandrin alcohol A, schisandrin alcohol B and schisandrin ester A is prepared by using methanol-formic acid aqueous solution.

[0029] In a specific embodiment, step S1 specifically includes:

[0030] S11. Weigh the schisandrin A standard, schisandrin B standard, schisandrin C standard, schisandrin alcohol A standard, schisandrin alcohol B standard and schisandrin ester A standard respectively, and prepare them into single standard stock solutions with a concentration of 10.00 ug / ML using methanol-formic acid aqueous solution.

[0031] S12. Take appropriate amount of single standard stock solution respectively, and prepare standard solution with gradient concentration of 0.01ug / ML to 5.00ug / ML by using methanol-formic acid aqueous solution.

[0032] S2. Prepare the test solution:

[0033] Sample pretreatment is a key step in the detection process and directly affects the subsequent LC-MS / MS analysis results, including:

[0034] S21. After drying the Schisandra chinensis sample, the sample is crushed in liquid nitrogen in the dark to obtain Schisandra chinensis powder.

[0035] In a specific embodiment, S21 specifically includes: after completely drying the Schisandra sample at 50° C. to 55° C., crushing it in liquid nitrogen in the dark and passing it through a 50-mesh sieve to obtain Schisandra powder.

[0036] S22. Adding methanol-formic acid aqueous solution to the Schisandra chinensis powder for ultrasonic extraction to obtain an extract; the solid-liquid ratio of the Schisandra chinensis powder to the methanol-formic acid aqueous solution is 1:15.

[0037] In a specific embodiment, the ultrasonic extraction time is 10 min to 30 min.

[0038] S23. Freeze the extract, centrifuge, and take the supernatant into a sampling bottle for analysis.

[0039] In a specific embodiment, the freezing temperature is -80°C; and the freezing time is 1 hour to 3 hours.

[0040] In a specific embodiment, the centrifugal temperature is 4°C, the centrifugal speed is 15000 r / min, and the centrifugal time is 5 min to 20 min. Specifically, low-temperature centrifugation helps to increase the precision of the detection method.

[0041] Furthermore, the volume percentage of formic acid in the methanol-formic acid aqueous solution in S1 and S2 is both 0.1%, and the volume percentage of methanol is both 80%.

[0042] Specifically, the present invention conducts in-depth research on the extraction method, and optimizes the extractant and the specific conditions of the treatment. The target components (SA, SB, SC, SLA, SLB, SNA) in the Schisandra chinensis sample to be tested can be quickly and efficiently extracted by using a methanol-formic acid aqueous solution, and combined with freezing and centrifugation, the protein and chlorophyll precipitated in the sample can be effectively removed. The method is simple and reliable, with high extraction efficiency, and can effectively reduce the interference of the sample matrix and impurity components on the LC-MS / MS measurement process, so that the extracted sample can meet the requirements of subsequent analysis, and the used extractant is cheap and low-toxic.

[0043] S3, LC-MS / MS detection:

[0044] S31. Detect the standard solution by LC-MS / MS to obtain a standard curve, and detect the test solution under the same conditions.

[0045] S32. Calculate the contents of the six lignan components in the test solution according to the standard curve;

[0046] In a specific embodiment, S3 specifically includes: sampling the standard solution, taking the peak area as the ordinate and the concentration as the abscissa, drawing a standard curve, sampling the test solution, testing the test solution under the same conditions, substituting the test results into the above standard curve, and obtaining the content of the 6 lignan components in the test solution. It should be noted that in the determination step of the content of the 6 lignan components in Schisandra chinensis of the present invention, the acquisition of the standard curve and the subsequent calculation of the specific component content according to the standard curve are existing technologies in the technical field, and the technical personnel in the technical field are capable of obtaining the technology and applying it to the determination step of the content of the 6 lignan components in Schisandra chinensis, which is a necessary skill for the technical personnel in the technical field, so the present invention will no longer describe the injection step and the calculation step outside the specific analysis conditions in detail.

[0047] Furthermore, the present invention screens the chromatographic detection conditions and the mass spectrometry detection conditions to optimize the detection method, and finally determines the chromatographic detection conditions and the mass spectrometry detection conditions as the following scheme:

[0048] The chromatographic detection conditions of LC-MS / MS are as follows: chromatographic column: ZORBAX RRHD Eclipse Plus-C18 chromatographic column, specification is 2.1mmx50mm, 1.8μm; mobile phase: A: aqueous solution containing 0.1% formic acid, B: acetonitrile solution containing 0.1% formic acid; gradient elution program: 0.0min, 45%B; 0.0~4.0min: 95%B; 4.0~5.0min: 95%B; 5.0~5.5min: 45%B; flow rate: 0.3mL / min; injection volume: 1.0μL; column temperature: 35℃; injection plate temperature 10℃;

[0049] The mass spectrometry detection conditions of LC-MS / MS are as follows: electrospray ion source; positive ion scanning mode; multiple reaction detection mode MRM; sheath gas, drying gas, and collision gas are all high-purity nitrogen; drying gas temperature: 350°C; drying gas flow rate: 7L / min; sheath gas temperature: 350°C; sheath gas flow rate: 12L / min; capillary voltage: 3500v; polarity: Positive; dwell time: 20ms; The mass spectrometry parameters of the six lignan components are shown in Table 1 below:

[0050] Table 1 Mass spectrometry parameters

[0051]

[0052] Specifically, the present invention establishes for the first time a method for simultaneously determining six lignan components (SA, SB, SC, SLA, SLB, and SNA) in Schisandra chinensis by LC-MS / MS. By adopting the above technical scheme and selecting specific analysis conditions, a rapid and accurate quantitative analysis of six main biphenylcyclooctene-type lignan components in Schisandra chinensis is achieved. The detection method of the present invention has the advantages of high sensitivity, good stability, accurate quantification, short detection time, low cost, low detection limit, and wide test range. It solves the technical problem that the prior art cannot simultaneously measure SA, SB, SC, SLA, SLB, and SNA in Schisandra chinensis, and provides a reliable high-throughput detection method for rapidly, efficiently, and accurately determining the contents of six components in different plants.

[0053] The following are specific embodiments

[0054] Example 1

[0055] 1. Experimental instruments and reagents

[0056] The test instrument used was: Agilent 1290 Infinity II liquid chromatography system UHPLC analyzer.

[0057] Schisandrin A: Horst Biotech (batch number DSTDW230225-008, purity ≥98%);

[0058] Schisandrin B: Horst Biotech (batch number DSTDW000901, purity ≥ 98%);

[0059] Schisandrin C: Horst Biotech (batch number DSTDW001401, purity ≥ 98%);

[0060] Schisandrin A: Horst Biotech (batch number DSTDW001001, purity ≥ 98%);

[0061] Schisandrin B: Horst Biotech (batch number DSTDW001301, purity ≥ 98%);

[0062] Schisandrin ester A: Horst Biotech (batch number DST230104-012, purity ≥98%);

[0063] Other extraction reagents were chromatographic grade reagents from Merck.

[0064] 2. Experimental steps

[0065] 2.1 Solution preparation

[0066] 2.1.1 Preparation of standard solutions: Weigh the standard products of schisandrin A, schisandrin B, schisandrin C, schisandrin alcohol A, schisandrin alcohol B and schisandrin ester A respectively, and prepare them into single standard stock solutions with a concentration of 10.00ug / ML using methanol-formic acid aqueous solution. Weigh appropriate amounts of the above single standard stock solutions respectively, and prepare them into standard solutions with gradient concentrations of 0.01ug / ML to 5.00ug / ML using methanol-formic acid aqueous solution.

[0067] 2.1.2 Prepare the test solution: After the Schisandra sample is completely dried at 50℃~55℃, it is crushed in liquid nitrogen in the dark and passed through a 50-mesh sieve to obtain Schisandra powder. Take 200 mg of Schisandra powder and add 3 mL of methanol-formic acid aqueous solution for ultrasonic extraction for 20 minutes to obtain an extract. After freezing the extract at 80℃ for 2 hours, centrifuge at 4℃ and 15000r / min for 10 minutes, take the supernatant into a sample injection bottle for analysis.

[0068] 2.2 Chromatographic and mass spectrometry conditions:

[0069] The chromatographic detection conditions of LC-MS / MS are as follows: chromatographic column: ZORBAX RRHD Eclipse Plus-C18 chromatographic column, specification is 2.1mmx50mm, 1.8μm; mobile phase: A: aqueous solution containing 0.1% formic acid, B: acetonitrile solution containing 0.1% formic acid; gradient elution program: 0.0min, 45%B; 0.0~4.0min: 95%B; 4.0~5.0min: 95%B; 5.0~5.5min: 45%B; flow rate: 0.3mL / min; injection volume: 1.0μL; column temperature: 35℃; injection plate temperature 10℃;

[0070] The mass spectrometry detection conditions of LC-MS / MS are as follows: electrospray ion source; positive ion scanning mode; multiple reaction detection mode MRM; sheath gas, drying gas, and collision gas are all high-purity nitrogen; drying gas temperature: 350°C; drying gas flow rate: 7L / min; sheath gas temperature: 350°C; sheath gas flow rate: 12L / min; capillary voltage: 3500v; polarity: Positive; dwell time: 20ms; The mass spectrometry parameters of the six lignan components are as follows Table 2:

[0071] Table 2 Mass spectrometry parameters

[0072]

[0073] The chromatograms and mass spectra of the six compounds in Schisandra chinensis measured under the above chromatographic and mass spectrometric conditions are shown in Figure 2 .

[0074] 2.3 Methodological investigation

[0075] 2.3.1 Investigation of linear relationship

[0076] A series of mixed standard solutions of 6 lignans with concentration gradients (0.01ug / ML, 0.05ug / ML, 0.10ug / ML, 0.125ug / ML, 0.25ug / ML, 0.50ug / ML, 1.00ug / ML, 1.25ug / ML, 2.50ug / ML, 5.00ug / ML) were measured under the above-mentioned chromatographic and mass spectrometric conditions, and the peak area-concentration standard curve was drawn and linear regression was performed to obtain the regression equation of the standard curve of the 6 lignan components. The signal-to-noise ratio method (S / N) was used to determine the quantitative limit of the instrument, and the quantitative limit (LOQ) was estimated with a 10-fold signal-to-noise ratio. The linear results are shown in Table 3. Table 3 is the results of the linear relationship investigation of the 6 lignan components.

[0077] Table 3 Results of linear relationship investigation of 6 lignan components

[0078] Analyte LOQ(ug / ML) Linear Rang(ug / ML) standard curv <![CDATA[R 2 ]]> SA 0.01 0.01-1.25 y = 13773x + 759.32 0.9970 SB 0.01 0.01-5 y = 93715x + 12251 0.9954 SC 0.01 0.01-5 y = 37659x + 1948.2 0.9979 SLA 0.01 0.01-5 y = 568147x + 269414 0.9984 SLB 0.01 0.01-5 y = 165019x + 15280 0.9992 SNA 0.01 0.01-0.25 y = 873712x + 2974.3 0.9988

[0079] As shown in Table 3, the six lignan components have a good linear relationship, and the correlation coefficient R 2 All of them were greater than 0.99, and the linear range of each lignan component was wide, which could meet the detection requirements.

[0080] 2.3.2 Stability study

[0081] The same sample solution was taken and measured according to the LC-MS / MS conditions under "2.2" at 0, 2, 4, 6, 8, 10, 12, and 24 hours. The relative standard deviations (RSD%, n = 3) of SA, SB, SC, SLA, SLB, and SNA were 0.06%, 2.10%, 0.30%, 0.16%, 0.10%, and 0.12%, respectively. The results showed that the sample solution was basically stable within 24 hours.

[0082] 2.4 Sample content determination

[0083] Accurately measure 5 Schisandra samples, prepare the test solution according to item "2.2.2", and determine it according to the LC-MS / MS conditions under item "2.2". The results of the contents of the 6 lignans in each Schisandra sample are shown in Table 4.

[0084] Table 4 Results of detection of 6 lignan components

[0085] Sample SA SB SC SLA SLB SNA 1 1.417 3.258 0.191 3.834 1.050 0.538 2 2.817 2.208 0.030 4.799 0.310 0.795 3 2.094 3.918 0.225 4.293 0.734 0.693 4 1.508 2.320 0.123 2.727 0.634 0.564 5 4.935 3.657 0.088 5.652 0.568 1.036

[0086] 3. Discussion on detection methods

[0087] 3.1 Optimization of chromatographic conditions

[0088] First, three chromatographic columns were investigated: ZORBAX RRHD Eclipse Plus-C 18 Chromatographic column, specification: 2.1mmx50mm, 1.8μm; Agilent Poroshell 120-SB C 18 Chromatographic column, size: 100 mm × 2.1 mm, 2.7 μm; and Shim-pack VP-ODS C 18 The chromatographic column with specifications of 250mm×4.6mm, 5μm was used to compare the peak shape and separation of the chromatographic peaks, and it was determined that the ZORBAX RRHD Eclipse Plus-C18 chromatographic column with specifications of 2.1mmx50mm, 1.8μm could achieve good separation and peak shape of the analytes; at the same time, the flow rate, gradient elution program, column temperature, injection plate temperature and different injection volumes were also investigated. The results showed that when the flow rate was 0.3mL / min, the column temperature was 35℃, the injection volume was 1.0μL, and the injection plate temperature was 10℃, the chromatographic peak shape of the six lignan components in Schisandra chinensis was better, and a good baseline separation could be achieved with the surrounding interfering substances.

[0089] 4. Optimization of mass spectrometry conditions

[0090] First, the six lignan components were prepared into 5.00ug / ML solutions for continuous injection by syringe pump to optimize mass spectrometry parameters. Quantitative analysis was performed using electrospray ionization source (ESI) and multiple reaction ion monitoring mode (MRM), and the ion peak intensity under positive and negative ion modes was compared. The results showed that the ion peak was more stable and had the best intensity under positive ion conditions. In positive ion mode, ion pairs were formed according to the selected parent ions and daughter ions, and MRM scans were performed to optimize each detection parameter.

[0091] 5. Extraction method optimization

[0092] A variety of solvents were screened for extracting 6 lignan components. The results showed that methanol-formic acid aqueous solution was the best extraction solvent in terms of extraction rate, solvent toxicity and economy. When determining the ultrasonic time, the lignan extraction effects at ultrasonic extraction times of 5, 10, 20, 30, 40, 50 and 60 minutes were compared, and the optimal ultrasonic extraction time was determined to be 20 minutes.

[0093] In summary, the present invention establishes for the first time a method for simultaneously determining six lignan components (SA, SB, SC, SLA, SLB, SNA) in Schisandra chinensis by LC-MS / MS, and realizes rapid and accurate quantitative analysis of six main biphenylcyclooctene-type lignan components in Schisandra chinensis by optimizing the detection parameters of extraction method, chromatography and mass spectrometry. The detection method of the present invention has the advantages of high sensitivity, good stability, accurate quantification, rapidity, low cost, low detection limit, etc.

[0094] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS, characterized in that: The lignan components include schisandrin A, schisandrin B, schisandrin C, schisandrin A, schisandrin B and schisandrin ester A; The method comprises the following steps: S1. Prepare standard solution: A standard solution containing schisandrin A, schisandrin B, schisandrin C, schisandrin alcohol A, schisandrin alcohol B and schisandrin ester A was prepared by using methanol-formic acid aqueous solution; S2. Prepare the test solution: After the Schisandra chinensis sample was dried, it was crushed in liquid nitrogen in the dark to obtain Schisandra chinensis powder; Adding methanol-formic acid aqueous solution to the Schisandra powder for ultrasonic extraction to obtain an extract; the material-liquid ratio of the Schisandra powder to the methanol-formic acid aqueous solution is 1:15; The extract is frozen, centrifuged, and the supernatant is placed in a sample injection bottle for analysis; The volume percentage of formic acid in the methanol-formic acid aqueous solution in S1 and S2 is 0.1%, and the volume percentage of methanol is 80%; S3, LC-MS / MS detection: The standard solution is detected by LC-MS / MS to obtain a standard curve, and the test solution is detected under the same conditions; Calculate the contents of the six lignan components in the test solution according to the standard curve; The chromatographic detection conditions of LC-MS / MS described in S3 are as follows: chromatographic column: ZORBAX RRHD Eclipse Plus-C18 chromatographic column, specification is 2.1mmx50mm, 1.8μm; mobile phase: A: aqueous solution containing 0.1% formic acid, B: acetonitrile solution containing 0.1% formic acid; gradient elution program: 0.0min, 45%B; 0.0~4.0min: 95%B; 4.0~5.0min: 95%B; 5.0~5.5min: 45%B; flow rate: 0.3mL / min; injection volume: 1.0μL; column temperature: 35℃; injection plate temperature 10℃; The mass spectrometry detection conditions of LC-MS / MS described in S3 are as follows: electrospray ion source; positive ion scanning mode; multiple reaction detection mode MRM; sheath gas, drying gas, and collision gas are all high-purity nitrogen; drying gas temperature: 350°C; drying gas flow rate: 7L / min; sheath gas temperature: 350°C; sheath gas flow rate: 12L / min; capillary voltage: 3500v; polarity: Positive; dwell time: 20ms; The mass spectrometry parameters of the six lignan components are as follows:

2. The method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The S1 specifically includes the following steps: Weigh the schisandrin A standard, schisandrin B standard, schisandrin C standard, schisandrin A standard, schisandrin B standard and schisandrin ester A standard respectively, and prepare a single standard stock solution with a concentration of 10.00 ug / ML using the methanol-formic acid aqueous solution; An appropriate amount of the single standard stock solution was measured respectively, and the methanol-formic acid aqueous solution was used to prepare a standard solution with a gradient concentration of 0.01 ug / ML to 5.00 ug / ML.

3. The method for rapid detection of 6 lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The drying temperature is 50°C to 55°C.

4. The method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The ultrasonic extraction time is 10 min to 30 min.

5. The method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The freezing temperature is -80°C; the freezing time is 1h to 3h.

6. The method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The centrifugal temperature is 4°C; The centrifugal speed is 15000 rpm; The centrifugation time is 5 min to 20 min.

7. The method for rapid detection of six lignan components in Schisandra chinensis based on LC-MS / MS according to claim 1, characterized in that: The dried Schisandra chinensis sample was ground in liquid nitrogen in the dark and passed through a 50-mesh sieve to obtain the Schisandra chinensis powder.

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