Method for efficiently obtaining wild cotesia brevifilis resources, collecting device and application

Abstract

The invention provides a method for efficiently obtaining wild cotesia brevifilis resources, a collecting device and application, and belongs to the technical field of biological control of agricultural pests, the method comprises the following steps that S1, the collecting device and host larvae are prepared, and the collecting device comprises a baffle plate and a net bag; s2, the host larvae are placed in a net bag, and the net bag is hung below the baffle plate; s3, hanging the cotesia testis collecting device in a wild cotesia testis activity area; s4, after it is observed that white cocoons grow in the net bag, the net bag is taken back; and S5, putting the mesh bag and the cocoons in the mesh bag into a culture room for culture to obtain the wild cotesia brevifilis adult bees. By the adoption of the collecting device, rapid and accurate collection of the cotesia giganteae can be achieved, specific host larvae are selected as baits according to a collecting place, eggs of the cotesia giganteae are parasitized on the host larvae, wild cotesia giganteae can be specifically collected, and other types of insects are prevented from being captured.

Description

Technical Field

[0001] The invention relates to the technical field of biological control of agricultural pests, and in particular to a method for efficiently obtaining wild Bombyx mori resources, a collecting device and an application thereof. Background Art

[0002] Parasitic wasps are an important biological resource in nature and play an important role in pest control. The gramineous wasp is a dominant external parasitic wasp of the tobacco meal moth and is widely distributed. In scientific research and artificial breeding of gramineous wasps, it is often necessary to collect a large number of wild germplasm resources for experimental research or population rejuvenation. The current method is mainly to artificially find wild queen bees and directly capture them using test tubes or worm suckers. The collection efficiency is low and the number is small, which is extremely inconvenient for subsequent development and utilization.

[0003] The Chinese patent application number 202123278731.7 discloses a parasitic bee collection and feeding device. The utility model consists of a feeding box located on the upper layer and an incubation box located on the lower layer. The food is placed between the feeding box and the incubation box partition to attract the parasitic bees in the lower incubation box through the bag opening to enter the upper feeding box. The device uses the parasitic bees' upward tendency and food tendency to achieve the aggregation and feeding of the existing bright-bellied enameled bee population, and the premise for its realization is to have bee species resources.

[0004] The Chinese patent application number 201510978715.9 discloses a device for collecting and preserving the wasp, including a collection chamber, a collection bottle, a breeding bottle and a bracket. A light source is provided on the upper part of the collection chamber. The invention uses the positive phototaxis of the wasp to collect bee species, and guides the wasp to enter and leave the breeding bottle by controlling the direction of the light source. Since many insects and parasitic wasps have phototaxis, the collection efficiency of this method is average, and the insects in the collection bottle need to be further screened and separated. Summary of the invention

[0005] In view of the technical problems existing in the background technology, the present application provides a method, a collection device and an application for efficiently obtaining wild B. pygma resources, which can realize the efficient and large-scale collection and application of wild B. pygma resources, reduce labor, and will not cause damage to the bee species themselves, nor cause negative impacts on other insects in the environment.

[0006] In a first aspect, the present invention provides a method for efficiently obtaining wild B. elegans resources, comprising the following steps: S1. prepare multiple collecting devices and a number of host larvae, wherein the collecting devices include shielding plates and net bags; S2, placing the host larvae in step S1 in a mesh bag, sealing the opening of the mesh bag, and hanging the mesh bag under the shield to obtain a collection device for the lycopodidae; S3, hanging the lycaenid collecting device in step S2 in the activity area of ​​wild lycaenids; S4, after observing that the host larvae in the collection device of the lycosa in step S3 have been parasitized by wild lycosa in the environment to lay eggs and develop into white cocoons, retrieve the net bag; S5. Place the net bag and the cocoons in the net bag in step S4 in a culture room for culture to obtain adult wild schizontidae.

[0007] In the technical solution of the embodiment of the present application, specific host larvae are preferentially selected according to the different areas where the collection device is suspended. The preferred principle is to select the main target population of wild lycopodiella in the area, for example, in the tobacco storage area, tobacco powder borer larvae are preferred, which will obtain more lycopodiella eggs than other hosts such as Indian meal borer, thereby obtaining more offspring lycopodiella.

[0008] Preferably, the host larvae in step S1 include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5th instar.

[0009] Preferably, the hanging height of the B. elegans collecting device in step 3 is 1.2 to 1.8 m, and the hanging time is 5 to 7 d.

[0010] Preferably, in step S5, the temperature in the culture chamber is 28-30° C., the relative humidity is 70%-80%, and the culture time is 3-5 days.

[0011] On the second aspect, an embodiment of the present application provides a collection device for efficiently obtaining wild wheat moth cocoon wasp resources. The net bag is made of a gauze with a mesh diameter of 0.12 to 0.20 mm. The net bag is flat and is provided with a bag opening, a sealing piece and a hanging rope. The bag opening is located above the net bag, and the sealing piece is used to seal the bag opening. The hanging rope is fixedly connected to both sides of the net bag. A lower hook is provided under the baffle, and the hanging rope of the net bag is fixedly connected to the lower hook.

[0012] In the technical solution of the embodiment of the present application, the mesh diameter of the net bag of the collecting device is specifically 0.12-0.20 mm, which can effectively ensure that the tobacco powder borer larvae placed therein will not escape from the net bag; and after the wild wheat moth bracona search for the tobacco powder borer larvae, they can use their tail needles to pass through the mesh holes of the net bag to paralyze the larvae and lay eggs, but the wheat moth bracona itself cannot penetrate the net bag, and other insects cannot enter the net bag, that is, the offspring wheat moth bracona formed after parasitization in the net bag cannot escape from the net bag during the entire collection and retrieval process; the flat shape of the net bag can ensure that the tobacco powder borer larvae do not overlap in the net bag, and the wheat moth bracona in the wild can paralyze and parasitize from the mesh holes on both sides of the net bag, thereby improving the parasitism efficiency.

[0013] In the third aspect, an embodiment of the present application provides an application of a wild meal moth wasp in propagation, wherein the meal moth wasp obtained in step S5 is parasitically cultured with a host, and the culture conditions are a temperature of 28°C~30°C, a relative humidity of 70%~80%, and a photoperiod of L:D=16:8, and the offspring meal moth wasp is obtained by culturing for 12~15 days.

[0014] Preferably, the ratio of the wasp to the host is 1:10~20.

[0015] Preferably, the host larvae include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5 to 6 instar.

[0016] In a third aspect, the present application embodiment provides an application of a wild lycopodiella in the rejuvenation of an artificially reared lycopodiella population, comprising the following steps: P1. The cocoons obtained in step S4 are individually packaged and placed in an environment with a temperature of 28° C. to 30° C. and a relative humidity of 70% to 80% to be cultured until they emerge, thereby obtaining adult wild larvae; P2, remove the weak wild female bees of Gnaphalidae in step P1, obtain strong wild female bees of Gnaphalidae, select strong male bees that have just emerged and have not mated in the artificially reared population, and mate with the strong wild female bees of Gnaphalidae one by one; P3. After 24 hours, the mated female bees in step P2 are parasitized with robust 5-6-year-old host larvae. After 12-15 days, a population of Lysimachia spp. rejuvenated by hybridization with wild Lysimachia spp. is obtained.

[0017] Preferably, the parasitic culture conditions in step P3 are a temperature of 28° C. to 30° C., a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8.

[0018] Different from the existing technical solutions, the beneficial effects of this application include: The collection device in the present application can realize the rapid and accurate collection of the wasp. According to the collection site, specific host larvae are selected as bait, so that the wasp parasitizes the eggs on the host larvae. Wild wasps can be specifically collected to avoid capturing other types of insects, and there is no need to further screen and separate the insects in the collection device. The host larvae are placed in the collection device, and the wasps in the warehouse or the wild will leave freely after parasitizing the eggs in the host larvae. The offspring bees collected are wild wasp. Compared with directly capturing and collecting wild wasps, the amount of wild bee resources obtained is increased by more than 10 times, the labor is greatly reduced, and the wild wasps will not be damaged during the collection process, nor will the wild wasp population in the environment be greatly reduced.

[0019] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solution of the present application, the following is a brief introduction to the drawings used in the present application. Obviously, the drawings described below are only some embodiments of the present application, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 This is a schematic diagram of the structure of the collection device in the embodiment of the present application.

[0022] Description of reference numerals: In the figure, 1. shielding plate; 11. upper hook; 12. lower hook; 2. net bag; 21. bag opening; 22. sealing piece; 23. hanging rope; 3. host larvae. DETAILED DESCRIPTION

[0023] The following embodiments of the technical solution of the present application are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" in the specification and claims of this application and the above-mentioned figure descriptions and any variations thereof are intended to cover non-exclusive inclusions.

[0025] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0026] In scientific research and artificial breeding of the lycopodidae, it is often necessary to collect a large number of wild germplasm resources for experimental research or population rejuvenation. The current method is mainly to manually find wild queen bees and directly capture them using test tubes or flukes. The collection efficiency is low and the number is small, which is extremely inconvenient for subsequent development and utilization.

[0027] In order to solve the technical problem that wild B. pygmaea is difficult to capture, the present application provides a method and application for efficiently obtaining wild B. pygmaea resources, which can realize the efficient and large-scale collection and application of wild B. pygmaea resources, reduce labor, and will not cause damage to the bee species itself, nor cause negative impacts on other insects in the environment.

[0028] Please refer to Figure 1 In the first aspect, the present invention provides a method for efficiently obtaining wild B. elegans resources, comprising the following steps: S1, preparing a plurality of collecting devices and a plurality of host larvae 3, wherein the collecting devices include a shielding plate 1 and a mesh bag 2; S2, placing the host larvae 3 in step S1 in the mesh bag 2, sealing the bag opening 21 of the mesh bag 2, and hanging the mesh bag 2 below the shielding plate 1 to obtain a collection device for the lycopodiella; S3, hanging the lycopodidae collecting device in step S2 in the tobacco storage warehouse area; S4, after observing that the host larvae 3 in the collection device of the lycosa in step S3 have been parasitized and laid eggs by wild lycosa in the environment and developed into white cocoons, the net bag 2 is retrieved; S5, placing the net bag 2 and the cocoons in the net bag 2 in step S4 in a culture room for culture, and obtaining adult wild schizontidae.

[0029] In the technical solution of the embodiment of the present application, specific host larvae are preferentially selected according to the different areas where the collection device is suspended. The preferred principle is to select the main target population of wild lycopodiella in the area, for example, in the tobacco storage area, tobacco powder borer larvae are preferred, which will obtain more lycopodiella eggs than other hosts such as Indian meal borer, thereby obtaining more offspring lycopodiella.

[0030] Preferably, the host larvae in step S1 include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5th instar.

[0031] In the technical solution of the embodiment of the present application, the host larvae used are 5th instar, which can effectively ensure that the host larvae will not pupate before being parasitized within 5-7 days, and can maximize the number and quality of offspring braconids obtained after parasitism. After each host larva is parasitized, 4-7 strong adult bees can be obtained.

[0032] Preferably, the hanging height of the B. elegans collecting device in step 3 is 1.2 to 1.8 m, and the hanging time is 5 to 7 d.

[0033] In the technical solution of the embodiment of the present application, the net bag remains stationary and suspended in the air before cocooning. After the host larvae in the net bag are parasitized, the eggs and larvae of the meal moth cocoon will not fall off the host larvae due to external vibration and be unable to obtain nutrition sources. At the same time, they are protected from attacks by ants and the like, effectively ensuring the survival rate of the meal moth cocoon.

[0034] Preferably, in step S5, the temperature in the culture chamber is 28-30° C., the relative humidity is 70%-80%, and the culture time is 3-5 days.

[0035] Secondly, an embodiment of the present application provides a collection device for efficiently obtaining wild Bombyx moth resources, including a baffle 1 and a net bag 2. The net bag 2 is made of a gauze with a mesh diameter of 0.12 to 0.20 mm. The net bag 2 is flat, and is provided with a bag opening 21, a sealing piece 22 and a hanging rope 23. The bag opening 21 is located above the net bag 2, and the sealing piece 22 is used to seal the bag opening 21. The hanging rope 23 is fixedly connected to both sides of the net bag 2. A lower hook 12 is provided under the baffle, and the hanging rope 23 of the net bag 2 is fixedly connected to the lower hook 12.

[0036] In the technical solution of the embodiment of the present application, the collecting device includes a baffle plate 1 and a mesh bag 2. The baffle plate 1 is used to block rain, sunlight, dust, etc. A bag opening 21 is provided above the mesh bag 2, and a sealing member 22 is provided at the bag opening 21. The sealing member 22 can be a heat-sealing member, and is sealed using a heat-sealing machine to prevent tobacco powder borer larvae in the mesh bag 2 from crawling out, or other insects from entering the mesh bag 2. The heat-sealing can keep the mesh bag 2 flat after sealing, and the bag opening 21 can be conveniently torn open later.

[0037] The mesh diameter of the net bag 2 of the collecting device is specifically 0.12-0.20 mm, which can effectively ensure that the placed host larvae 3 will not escape from the net bag 2; and after the wild meal moth cocoon wasps search for the host larvae 3, they can use their tail needles to pass through the mesh of the net bag 2 to paralyze the larvae and lay eggs, but the meal moth cocoon wasps themselves cannot penetrate the net bag 2, that is, the offspring meal moth cocoon wasps formed after being parasitized in the net bag 2 cannot escape from the net bag 2 during the entire collection and retrieval process.

[0038] A lower hook 12 is provided under the shielding plate 1, and the net bag 2 is hung on the lower hook 12 after being sealed. An upper hook 11 is provided above the shielding plate 1, which is used to hang the shielding plate 1 and the net bag 2 in the activity area of ​​wild wheat moth wasps, such as a tobacco warehouse or in the wild. The net bag 2 is exposed to the air, which is convenient for wild wheat moth wasps to search for host larvae 3. The flat shape of the net bag 2 can ensure that the tobacco powder borer larvae do not overlap in the net bag 2. The wild wheat moth wasps can paralyze and parasitize from the mesh holes on both sides of the net bag 2, thereby improving the parasitism efficiency.

[0039] In the third aspect, the present application embodiment provides an application of a wild lycopodiella in propagation, wherein the lycopodiella obtained in step S5 is transferred from the mesh bag to a breeding vessel containing a host for cultivation, and the culture conditions are a temperature of 28°C to 30°C, a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8, and the offspring lycopodiella is obtained by culturing for 12 to 15 days. The target pests of the lycopodiella propagated in this embodiment are mainly tobacco powder borers in tobacco storage warehouses.

[0040] Preferably, the ratio of the wasp to the host is 1:10-20.

[0041] Preferably, the host larvae include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5 to 6 instar.

[0042] In the technical solution of the embodiment of the present application, the emergence rate of host larvae below 5 years old is not high, and the adult bees are not strong. By using host larvae of 5 to 6 years old to cultivate the meal moth braconid wasp, strong adult bees can be obtained.

[0043] In a third aspect, the present application embodiment provides an application of a wild lycopodiella in the rejuvenation of an artificially reared lycopodiella population, comprising the following steps: P1. The cocoons obtained in step S4 are individually packed into finger-shaped tubes, and the finger-shaped tubes are placed in an environment with a temperature of 28° C. to 30° C. and a relative humidity of 70% to 80% and cultured until they emerge, thereby obtaining wild larvae; P2. Remove the finger-shaped tubes containing weak wild female bees of the meal moth braconid in step P1, obtain the finger-shaped tubes containing strong wild female bees of the meal moth braconid, select the newly emerged strong male bees that have not been mated in the artificial breeding population, and put them into the finger-shaped tubes with strong female bees one by one for mating; P3. After 24 hours, the healthy host larvae are placed in the finger-shaped tube containing the mated female bees in step P2 for parasitic culture. After 12 to 15 days, the population of Lysimachia spp. rejuvenated by hybridization with wild Lysimachia spp. is obtained.

[0044] Preferably, the parasitic culture conditions in step P3 are a temperature of 28° C. to 30° C., a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8.

[0045] In the technical scheme of the embodiments of the present application, the intermediate host selected for propagation and rejuvenation is mainly determined according to the biological control target and the effect of large-scale breeding. The target pests in tobacco fields are mainly tobacco powder borers, and the effect of breeding bees with tobacco powder borers is good. Therefore, the biological control effect of bees obtained directly using tobacco powder borers as intermediate hosts is better than that of bees obtained with other intermediate hosts.

[0046] Some specific embodiments are listed below. It should be noted that the embodiments described below are exemplary and are only used to explain the present application, and should not be construed as limiting the present application. If specific techniques or conditions are not specified in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used without specifying the manufacturer are all conventional products that can be obtained commercially.

[0047] 1. Preparation method Example 1 A method for efficiently obtaining wild Bryophora sphaerocephala resources, characterized in that it comprises the following steps: S1. Prepare a collecting device and five fifth-instar tobacco moth larvae. The collecting device includes a baffle and a mesh bag. The mesh bag is made of a gauze with a mesh diameter of 0.12 mm. The mesh bag is flat and has a sealing piece on the top. A lower hook is provided under the baffle, and the mesh bag is hung on the lower hook. S2, placing the tobacco powder borer larvae in step S1 in a mesh bag, sealing the mesh bag opening with a heat sealer, and hanging the mesh bag below the shielding plate to obtain a collection device for the lycopodidae; S3, the lycopodiella collecting device in step S2 is hung in the tobacco storage area of ​​the tobacco sales center in Enshi City, Hubei Province, with a hanging height of 1.8m, and each mesh bag is filled with 5 5th instar larvae of tobacco powder borer, and the tobacco powder borer larvae in the mesh bag can be accurately located by the lycopodiella in the environment, and the hanging time is 7d; S4. After 7 days, it was observed that the net bag was infested with tobacco powder borer, and there were 27 white cocoons in the net bag, so the net bag was retrieved; S5. The mesh bag and the cocoons in the mesh bag in step S4 are placed in a culture room for cultivation. The temperature in the culture room is 28-30° C., the relative humidity is 70%-80%, and the cultivation time is 3-5 days. The cocoons in the mesh bag gradually emerge into adult bees, and 26 adult wild Lymea fasciata bees are obtained.

[0048] Example 2 A method for efficiently obtaining wild Bryophora sphaerocephala resources, characterized in that it comprises the following steps: S1. Prepare a collecting device and five fifth-instar tobacco moth larvae. The collecting device includes a baffle and a mesh bag. The mesh bag is made of a gauze with a mesh diameter of 0.12 mm. The mesh bag is flat and has a sealing piece on the top. A lower hook is provided under the baffle, and the mesh bag is hung on the lower hook. S2, placing the tobacco powder borer larvae in step S1 in a mesh bag, sealing the mesh bag opening with a heat sealer, and hanging the mesh bag below the shielding plate to obtain a collection device for the lycopodidae; S3, the wheat moth braconid wasp collecting device in step S2 is respectively hung in the tobacco leaf purchasing group warehouse of Fenshui Village, Sancha Town, with a hanging height of 1.8m, and each mesh bag is provided with 5 5th instar larvae of tobacco powder borer, and the tobacco powder borer larvae in the mesh bag can be accurately located by the wheat moth braconid wasp in the environment, and the hanging time is 7d; S4. After 7 days, it was observed that the net bag was parasitized by tobacco powder borer, and there were 21 white cocoons in the net bag, so the net bag was retrieved; S5. The mesh bag and the cocoons in the mesh bag in step S4 are placed in a culture room for cultivation. The temperature in the culture room is 28-30° C., the relative humidity is 70%-80%, and the cultivation time is 3-5 days. The cocoons in the mesh bag gradually emerge into adult bees, and 21 adult wild Lygus elegans bees are obtained.

[0049] Example 3 An application of a wild lycopodidae wasp in propagation, comprising the following steps: The wild adult bees of the lycopodiella obtained in Example 1 were taken out from the net bag and transferred to a breeding vessel containing 5-instar larvae of tobacco powder borer. The ratio of the lycopodiella to the tobacco powder borer larvae was 1:10. The breeding environment was 28°C to 30°C, the relative humidity was 70% to 80%, and the photoperiod L:D=16:8. After 12 to 15 days, the offspring lycopodiella was obtained. Every 48 hours, the lycopodiella was transferred to a new breeding vessel containing 5-instar larvae of tobacco powder borer to continue parasitizing. A large number of offspring lycopodiella were obtained 12 to 15 days after the tobacco powder borer larvae were parasitized.

[0050] Example 4 The invention discloses an application of a wild ophidioptera in the rejuvenation of an artificially reared ophidioptera population, comprising the following steps: P1. After cutting the cocoons of the wild lycopodiella in step S4 of Example 1 together with the gauze, they are individually packaged into finger-shaped tubes, and the finger-shaped tubes are placed in an environment with a temperature of 28° C. to 30° C. and a relative humidity of 70% to 80% and cultured until they emerge, to obtain wild lycopodiella; P2. Remove the finger-shaped tubes containing weak wild female bees of the genus Mylotrichum spp., select strong male bees that have just emerged and have not mated from the artificially reared population, and place them one by one into the finger-shaped tubes with strong female bees for mating; P3. After 24 hours, the robust 5th-instar tobacco powder borer larvae were placed in the finger-shaped tube containing the mated female bees in step P2 for parasitic culture. The parasitic culture conditions were a temperature of 28°C to 30°C, a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8. After 12 to 15 days, a population of P. pygmaea rejuvenated by hybridization with wild P. pygmaea was obtained.

[0051] From 2021 to the present, the natural enemy insect breeding center and expansion base of Enshi State Company of Hubei Tobacco Company have been using the technology in Example 4 to carry out artificial propagation and population rejuvenation of the wheat moth braconid wasp with tobacco powder borer as the host, and the bee production was nearly 20 million in 2024. The results of the test on the artificially reared population showed that the parasitism rate of the existing population to tobacco powder borer was basically the same as the parasitism rate at the beginning of the rearing in 2019, indicating that the wheat moth braconid wasps obtained from the artificially reared population were as strong as the wild wheat moth braconid wasps captured in 2019.

[0052] Comparative Example 1 The experimental scheme of Comparative Example 1 and Example 1 was carried out at the same place and at the same time. In Comparative Example 1, a wild lycopodiella was manually searched in the tobacco storage area of ​​the tobacco sales center in Enshi City, Hubei Province using a flashlight and an insect sucker, and 1 was collected. The lycopodiella wasp is tiny and difficult to search with the naked eye. Manual search is inefficient, time-consuming and labor-intensive.

[0053] It should be noted that the present application is not limited to the above-mentioned embodiments. The above-mentioned embodiments are only examples, and the embodiments having the same structure as the technical idea and exerting the same effect within the scope of the technical solution of the present application are all included in the technical scope of the present application. In addition, without departing from the scope of the main purpose of the present application, various modifications that can be thought of by those skilled in the art to the embodiments and other methods of combining some of the constituent elements in the embodiments are also included in the scope of the present application.

Claims

1. A method for efficiently obtaining wild Bryophora spp. resources, characterized in that: The steps include: S1, preparing a plurality of collecting devices and a plurality of host larvae (3), wherein the collecting devices include a shielding plate (1) and a mesh bag (2); S2, placing the host larvae (3) in step S1 in a mesh bag (2), sealing the bag opening (21) of the mesh bag (2), and hanging the mesh bag (2) below the shielding plate (1), thereby obtaining a device for collecting the larvae of the meal moth; S3, hanging the lycaenid collecting device in step S2 in the activity area of ​​wild lycaenids; S4, after observing that the host larvae (3) in the collection device of the lycosa in step S3 have been parasitized by wild lycosa in the environment and laid eggs, and developed into white cocoons, the net bag (2) is retrieved; S5, placing the net bag (2) and the cocoons in the net bag (2) in step S4 in a culture room for culture, and obtaining adult wild larvae.

2. The method for efficiently obtaining wild Bryophora spp. resources according to claim 1, characterized in that: The host larvae in step S1 include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5th instar.

3. The method for efficiently obtaining wild Bryophora spp. resources according to claim 1, characterized in that: The hanging height of the collection device of the larvae moth in the step 3 is 1.2 to 1.8 m, and the hanging time is 5 to 7 days.

4. The method for efficiently obtaining wild Bryophora spp. resources according to claim 1, characterized in that: In step S5, the temperature in the culture chamber is 28-30° C., the relative humidity is 70%-80%, and the culture time is 3-5 days.

5. A collection device for efficiently obtaining wild B. elegans resources according to any one of claims 1 to 4, characterized in that: The invention comprises a baffle plate (1) and a mesh bag (2), wherein the mesh bag (2) is made of a gauze with a mesh diameter of 0.12 to 0.20 mm, the mesh bag (2) is flat, and is provided with a bag opening (21), a sealing member (22) and a hanging rope (23), the bag opening (21) is located above the mesh bag (2), the sealing member (22) is used to seal the bag opening (21), the hanging rope (23) is fixedly connected to both sides of the mesh bag (2), a lower hook (12) is provided below the baffle plate, and the mesh bag (2) is fixedly connected to the lower hook (12) via the hanging rope (23).

6. The use of a wild Lycopodiella spp. in propagation according to any one of claims 1 to 4, characterized in that: The offspring of the lycaenid wasp obtained in step S5 are parasitically cultured with a host under the culture conditions of a temperature of 28°C to 30°C, a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8, and the offspring is obtained by culturing for 12 to 15 days.

7. The use of wild Lycopodiella spp. in propagation according to claim 6, characterized in that: The ratio of the wasp to the host is 1:10-20.

8. The use of wild Lycopodiella spp. in propagation according to claim 6, characterized in that: The host larvae include one or more of tobacco powder borer larvae, powdery mildew borer larvae, and Indian meal borer larvae, and the host larvae are 5-6 years old.

9. The use of a wild lycosa spp. according to any one of claims 1 to 4 in the rejuvenation of an artificially reared lycosa spp. population, characterized in that: The steps include: P1. The cocoons obtained in step S4 are individually packaged and placed in an environment with a temperature of 28° C. to 30° C. and a relative humidity of 70% to 80% to be cultured until they emerge, thereby obtaining adult wild larvae; P2, remove the weak wild female bees of Gnaphalidae in step P1, obtain strong wild female bees of Gnaphalidae, select strong male bees that have just emerged and have not mated in the artificially reared population, and mate with the strong wild female bees of Gnaphalidae one by one; P3. After 24 hours, the mated female bees in step P2 are parasitized with robust 5-6-year-old host larvae. After 12-15 days, a population of Lysimachia spp. rejuvenated by hybridization with wild Lysimachia spp. is obtained.

10. The use of wild Lycopodiella spp. in the rejuvenation of artificially reared Lycopodiella spp. population according to claim 9, characterized in that: The parasitic culture conditions in step P3 are a temperature of 28° C. to 30° C., a relative humidity of 70% to 80%, and a photoperiod of L:D=16:8.

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