Screening device for microorganisms in yeast for making hard liquor

By designing a microbial screening device containing a support frame and limit wheel, adjusting the oscillation frequency and inclination angle of the triangle flask, the problem that existing oscillators cannot adjust the inclination angle is solved, and the growth and metabolic activities of Daqu microorganisms are improved, thus achieving the realization of the growth and metabolic activities of Daqu microorganisms. Improves oxygen transfer and stability.

CN119193278APending Publication Date: 2024-12-27MOUTAI INST
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Patent Information

Application Number
CN202411453552.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-17
Publication Date
2024-12-27

AI Technical Summary

Technical Problem

The oscillator in existing laboratories cannot adjust the inclination angle when the triangular flask is oscillated, affecting the growth and metabolic activities of Daqu microorganisms.

Method used

A microbial screening device including a support frame, a rotary rod, a motor, a transmission wheel and a limit wheel is designed. By adjusting the position of the transmission wheel, the inclination angle of the limit wheel is controlled, and the inclination angle of the triangular flask is adjusted. The range is controlled from 0° to 30°.

Benefits of technology

The oscillation frequency and inclination angle of the triangle flask are effectively adjusted, oxygen transfer is improved, excessive foam formation is avoided, and microbial growth and metabolic activities are promoted.

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Abstract

The scheme discloses a screening device for microorganisms in yeast for making hard liquor in the field of white liquor brewing, the screening device comprises a supporting frame, a rotating rod is rotationally connected to the supporting frame, the rotating rod is connected with a motor for driving the rotating rod to rotate, the rotating rod is sleeved with a transmission wheel and a limiting wheel, and the rotating rod is provided with a positioning strip for limiting transverse rotation of the transmission wheel; the transmission wheel is connected with a driver which vertically moves on the positioning strip, the limiting wheel is rotationally connected with the rotating rod, a connecting rod is hinged between the transmission wheel and the limiting wheel, and the limiting wheel is connected with a fixing base. According to the scheme, the inclination angle of the conical flask during oscillation can be adjusted.
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Description

Technical Field

[0001] The invention relates to the field of liquor brewing, and in particular to a device for screening microorganisms in Daqu. Background Art

[0002] Daqu is one of the core raw materials for brewing traditional Chinese liquor. It cultivates a rich microbial community through natural fermentation, including bacteria, yeasts, molds and other microorganisms. These microorganisms play a vital role in the fermentation process of Daqu. They not only start the fermentation reaction, but also directly affect the flavor, quality and nutritional value of the liquor. The microorganisms in Daqu can decompose complex organic matter and produce various metabolites, such as organic acids such as lactic acid and acetic acid, as well as flavor substances such as alcohols and esters, giving the liquor a unique aroma and taste.

[0003] In order to optimize the quality of liquor and improve production efficiency, microbial screening has become a key link in research. By screening out microorganisms with specific functions, such as efficient acid-producing bacteria, aroma-producing bacteria, or yeasts with excellent fermentation performance, the flavor and stability of liquor can be significantly improved.

[0004] The applicant screened microorganisms when conducting the screening, identification and enzyme production condition optimization research of high temperature resistant cellulose degrading bacteria. In the microbial screening experiment, the use of Erlenmeyer flasks for oscillation culture is a common method. Oscillation culture helps to improve the growth rate and metabolic activity of microorganisms because it can promote the uniform distribution of nutrients and oxygen in the culture medium. The applicant found that in the process of screening high temperature resistant cellulose degrading bacteria, the tilt angle during oscillation has an important influence on the screening of such bacteria. The appropriate tilt angle can increase the contact area between the liquid surface and the air, thereby increasing the dissolution rate of oxygen. This is particularly important for aerobic microorganisms, especially those cellulose degrading bacteria that are active under high temperature conditions. A larger tilt angle may cause the cellulose particles in the culture medium to settle, which is not conducive to the contact and utilization of the substrate by the microorganism. However, the oscillators in existing laboratories do not have the function of adjusting the oscillation frequency, which will have an adverse effect on the growth and metabolic activity of Daqu microorganisms. Summary of the invention

[0005] The present invention is intended to provide a screening device for microorganisms in Daqu, so as to solve the problem that the existing oscillator cannot adjust the tilt angle of the conical flask during oscillation.

[0006] A screening device for microorganisms in Daqu in the present scheme includes a support frame, a rotating rod is rotatably connected to the support frame, the rotating rod is connected to a motor for driving rotation, a transmission wheel and a limiting wheel are sleeved on the rotating rod, a positioning bar for limiting the lateral rotation of the transmission wheel is provided on the rotating rod, the transmission wheel is connected to a driver for vertical movement on the positioning bar, the limiting wheel is rotatably connected to the rotating rod, a connecting rod is hinged between the transmission wheel and the limiting wheel, and a fixed seat is connected to the limiting wheel.

[0007] The working principle and beneficial effects of this solution are as follows: when in use, the crushed Daqu sample is added to a conical flask filled with sterile saline, and glass beads are added to help mix. The conical flask is then fixed to a fixed seat, and the motor is turned on. The rotation of the motor drives the rotating rod to rotate, and the rotation of the rotating rod drives the transmission wheel and the limiting wheel to rotate together. The rotation of the limiting wheel can drive the fixed seat to rotate, so that the conical flask fixed on the fixed seat can be oscillated and shaken. When the oscillation frequency needs to be adjusted, the driver is started, and the driver drives the transmission wheel to move vertically on the rotating rod under the restriction of the positioning bar. While moving vertically, the transmission wheel still rotates with the rotating rod. The transmission wheel moves vertically and pulls the connecting rod, thereby driving the limiting wheel to tilt the position connected to the connecting rod upward or downward, so that the inclination of the fixed seat can be adjusted, and the inclination angle of the conical flask during oscillation can be controlled, and the inclination angle is controlled between 0° and 30°. Such an inclination angle can ensure sufficient oxygen transfer without causing excessive foam formation. The device in this scheme controls the inclination angle of the limiting wheel by adjusting the position of the transmission wheel, and the rotation of the transmission wheel and the limiting wheel with the rotating rod is not affected when the angle is adjusted.

[0008] Furthermore, the support frame includes a base, to which a side plate is fixedly connected, and the side plate is fixedly and vertically connected to a support plate.

[0009] Furthermore, the driver includes a threaded rod, which passes through the support plate and is threadedly connected to the support plate, the threaded rod is fixedly connected to an adjusting wheel, the outer wall of the transmission wheel is ringed with a groove, the adjusting wheel is located in the groove, and the bottom end of the threaded rod is fixedly connected to an adjusting handle. When in use, the adjusting handle is manually rotated to drive the threaded rod fixed thereto to rotate, the rotation of the threaded rod drives the adjusting wheel fixed thereto to rotate, and the adjusting wheel drives the transmission wheel to move up and down.

[0010] Furthermore, the ends of the transmission wheel and the limiting wheel close to each other are both provided with connecting blocks, and the two ends of the connecting rod are respectively hinged to the connecting blocks. When the end of the connecting rod connected to the transmission wheel moves, the limiting wheel will be pulled to tilt.

[0011] Furthermore, a positioning rod is passed through the rotating rod, and the two ends of the positioning rod are passed through the limiting wheels. This arrangement enables the limiting wheels to be rotatably connected to the rotating rod and is easy to disassemble.

[0012] Furthermore, a plurality of fixing rods are fixedly connected between the limiting wheel and the fixing seat, and the fixing seat is fixed by the fixing rods.

[0013] Furthermore, the fixing base is provided with a positioning groove, and a plurality of elastic ropes are staggeredly connected to the fixing base. When in use, the elastic ropes are pushed aside, the conical flask is placed in the positioning groove, and the elastic ropes are released, and the staggered elastic ropes can fix the conical flask.

[0014] Furthermore, the side plate is provided with a slide groove, a slider is slidably connected in the slide groove, a connecting rod is fixedly connected to the slider, a sliding ball is fixedly connected to the connecting rod, a limiting groove is provided on the outer wall ring of the limiting wheel, and the sliding ball is slidably connected in the limiting groove. The sliding ball can limit the limiting wheel, and if one end of the limiting wheel moves beyond the range, the sliding ball moves up and down driven by the limiting wheel, which will drive the slider to move up and down in the opposite direction of the sliding ball. The sliding block is slidably connected in the slide groove to limit the movement of the sliding ball, thereby making the deflection of the limiting wheel more stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic structural diagram of a device for screening microorganisms in Daqu according to the present invention;

[0016] Figure 2 for Figure 1 A top view of the middle limit wheel;

[0017] Figure 3 This is a schematic diagram of the structure of Example 2. DETAILED DESCRIPTION

[0018] The following is a further detailed description through specific implementation methods:

[0019] The figure marks in the drawings of the specification include: base 1, side plate 2, support plate 3, motor 4, adjustment handle 5, threaded rod 6, adjustment wheel 7, rotating rod 8, transmission wheel 9, groove 10, positioning bar 11, connecting block 12, connecting rod 13, limiting wheel 14, positioning rod 15, fixing rod 16, fixing seat 17, slide groove 18, slider 19, connecting rod 20, sliding ball 21.

[0020] Example 1

[0021] Basically as attached Figure 1 and 2As shown: a screening device for microorganisms in Daqu, including a support frame, the support frame includes a base 1, the base 1 is fixedly connected with a side plate 2, the side plate 2 is fixedly and vertically connected with a support plate 3, the support plate 3 is rotatably connected with a rotating rod 8, the rotating rod 8 is connected with a motor 4 for driving rotation, the motor 4 is fixedly connected to the base 1, a transmission wheel 9 and a limiting wheel 14 are sleeved on the rotating rod 8, a positioning bar 11 for limiting the lateral rotation of the transmission wheel 9 is provided on the rotating rod 8, the transmission wheel 9 is connected with a driver for vertical movement on the positioning bar 11, the driver includes a threaded rod 6, the threaded rod 6 passes through the support plate 3 and is threadedly connected to the support plate 3, and the upper end of the threaded rod 6 is fixedly connected with an adjusting wheel 7, The outer wall ring of the transmission wheel 9 is provided with a groove 10, and the adjusting wheel 7 is slidably connected in the groove 10. The lower end of the threaded rod 6 is fixedly connected with the adjusting handle 5. A positioning rod 15 passes through and is slidably connected to the rotating rod 8. Both ends of the positioning rod 15 pass through the limiting wheel 14, and the limiting wheel 14 is rotatably connected with the rotating rod 8 through the positioning rod 15. The ends of the transmission wheel 9 and the limiting wheel 14 that are close to each other are both provided with a connecting block 12, and a connecting rod 13 is hinged between the two connecting blocks 12; a fixing seat 17 is connected to the limiting wheel 14, and a plurality of fixing rods 16 are fixedly connected between the limiting wheel 14 and the fixing seat 17, and a positioning groove is provided on the fixing seat 17, and a plurality of elastic ropes are staggeredly connected to the fixing seat 17.

[0022] When in use, add the crushed Daqu sample into a conical flask filled with sterile physiological saline, and add glass beads to help mix, then push aside the elastic rope, place the conical flask into the positioning groove, release the elastic rope, and the staggered elastic ropes can fix the conical flask, turn on the motor 4, the motor 4 rotates to drive the rotating rod 8 to rotate, the rotating rod 8 rotates to drive the transmission wheel 9 and the limiting wheel 14 to rotate together, the limiting wheel 14 rotates to drive the fixing seat 17 to rotate, so that the conical flask fixed on the fixing seat 17 can be oscillated and shaken, when the oscillation frequency needs to be adjusted, the adjusting handle 5 is turned by hand, thereby driving the threaded rod 6 fixed thereto to rotate, and the threaded rod 6 The rotation drives the adjusting wheel 7 fixedly connected thereto to rotate, and the adjusting wheel 7 drives the transmission wheel 9 to move up and down, and the driver drives the transmission wheel 9 to move vertically on the rotating rod 8 under the restriction of the positioning bar 11. While moving vertically, the transmission wheel 9 still rotates with the rotating rod 8, and the transmission wheel 9 moves vertically to pull the connecting rod 13, thereby driving the limiting wheel 14 to tilt upward or downward at the position connected to the connecting rod 13, so that the inclination of the fixed seat 17 can be adjusted, and the inclination angle of the Erlenmeyer flask during oscillation can be controlled, and the inclination angle can be controlled between 0° and 30°. Such an inclination angle can ensure sufficient oxygen transfer without causing excessive foam formation. The device in this scheme controls the inclination angle of the limiting wheel 14 by adjusting the position of the transmission wheel 9, and the rotation of the transmission wheel 9 and the limiting wheel 14 with the rotating rod 8 is not affected when the angle is adjusted.

[0023] Example 2

[0024] Basically as attached Figure 3 The difference between the embodiment shown and the embodiment 1 is that: a slide groove 18 is provided on the side plate 2, a slider 19 is slidably connected in the slide groove 18, a connecting rod 20 is fixedly connected to the slider 19, a sliding ball 21 is fixedly connected to the connecting rod 20, a limiting groove is provided on the outer wall ring of the limiting wheel 14, and the sliding ball 21 is slidably connected in the limiting groove. The sliding ball 21 can limit the limiting wheel 14, and if one end of the limiting wheel 14 moves beyond the range, the sliding ball 21 moves up and down under the drive of the limiting wheel 14, which will drive the slider 19 to move up and down in the opposite direction of the sliding ball 21. The sliding block 19 is slidably connected in the slide groove 18 to limit the movement of the sliding ball 21, so that the deflection of the limiting wheel 14 is more stable.

[0025] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A device for screening microorganisms in Daqu, characterized in that: It includes a support frame, which is rotatably connected to a rotating rod, the rotating rod is connected to a motor for driving rotation, a transmission wheel and a limiting wheel are sleeved on the rotating rod, a positioning bar for limiting the lateral rotation of the transmission wheel is provided on the rotating rod, the transmission wheel is connected to a driver for vertical movement on the positioning bar, the limiting wheel is rotatably connected to the rotating rod, a connecting rod is hinged between the transmission wheel and the limiting wheel, and a fixing seat is connected to the limiting wheel.

2. The device for screening microorganisms in Daqu according to claim 1, characterized in that: The support frame comprises a base, a side plate is fixedly connected to the base, and a support plate is fixedly and vertically connected to the side plate.

3. The device for screening microorganisms in Daqu according to claim 2, characterized in that: The driver includes a threaded rod, which passes through the support plate and is threadedly connected to the support plate. The threaded rod is fixedly connected to an adjusting wheel. The outer wall ring of the transmission wheel is provided with a groove, the adjusting wheel is located in the groove, and the bottom end of the threaded rod is fixedly connected to an adjusting handle.

4. The device for screening microorganisms in Daqu according to claim 3, characterized in that: The ends of the transmission wheel and the limiting wheel that are close to each other are both provided with connecting blocks, and the two ends of the connecting rod are respectively hinged to the connecting blocks.

5. The device for screening microorganisms in Daqu according to claim 4, characterized in that: A positioning rod is passed through the rotating rod, and two ends of the positioning rod pass through the limiting wheels.

6. The device for screening microorganisms in Daqu according to claim 5, characterized in that: A plurality of fixing rods are fixedly connected between the limiting wheel and the fixing seat.

7. The device for screening microorganisms in Daqu according to claim 6, characterized in that: The fixing seat is provided with a positioning groove, and a plurality of elastic ropes are staggeredly connected to the fixing seat.

8. The device for screening microorganisms in Daqu according to claim 7, characterized in that: The side plate is provided with a slide groove, a slider is slidably connected in the slide groove, a connecting rod is fixedly connected to the slider, a sliding ball is fixedly connected to the connecting rod, a limiting groove is provided on the outer wall ring of the limiting wheel, and the sliding ball is slidably connected in the limiting groove.