A fermentation and cultivation device for nematode-resistant microbial inoculum
By designing a fermentation and culture equipment for microbial fermentation and cultivation of nematode anti-nematode microbial agents for microbial fermentation, and using intermittent release, agitation and oxygen enhancement components, the problems of difficult carbon source delivery, easy airtightness and insufficient oxygen contact in existing equipment are solved, and an automated and quantitative fermentation process and efficient product quality are achieved.
Patent Information
- Application Number
- CN202411475091.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-22
- Publication Date
- 2025-06-17
- Estimated Expiration
- 2044-10-22
AI Technical Summary
When existing fermentation equipment performs microbial fermentation, it is difficult to release carbon sources, and the airtightness is easily destroyed, resulting in a decrease in fermentation rate and product quality, and insufficient oxygen contact, so that microbial cells are easily damaged by stirred leaves.
A fermentation and culture equipment for anti-nematode microbial agents is designed, and the intermittent delivery component is used to realize automatic quantitative carbon source delivery. The agitating component improves fluidity and mixing uniformity through the reciprocating swing of multiple rotating blocks, and the oxygen-enhancing component directly provides oxygen through the air outlet pipe.
It realizes automated and quantitative carbon source delivery, improves the fluidity of microbial agents and the contact of nutrients, provides sufficient oxygen supply, and avoids equipment airtight damage and microbial cell damage.
Smart Images

Figure CN119193279B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of microbial inoculum fermentation culture, and specifically to a fermentation culture device for nematode-resistant microbial inoculum. Background Art
[0002] Microbial fermentation refers to the process of using microorganisms to convert raw materials into products required by humans through specific metabolic pathways under suitable conditions. The production level of microbial fermentation mainly depends on the genetic characteristics of the strains themselves and the culture conditions, and is widely used in industries such as the pharmaceutical industry, food industry, energy industry, chemical industry, and agriculture. Nematode-resistant microbial inoculum is a biological agent that uses beneficial microorganisms to control plant parasitic nematodes such as root-knot nematodes.
[0003] A fermentation device refers to a device used for microbial fermentation that can withstand steam sterilization.
[0004] When the existing fermentation devices carry out microbial fermentation, it is necessary to batch-feed carbon sources according to different situations. Currently, when feeding carbon sources, it is carried out by manually opening the device multiple times, which increases the feeding difficulty. Moreover, opening the device multiple times will damage the airtightness of the fermentation system, causing gas leakage, thereby affecting the fermentation rate and the quality of fermentation products. During the fermentation process, it is necessary to timely introduce oxygen into the device. Currently, oxygen is directly injected into the device, and it is impossible to inject oxygen during the stirring process to enable better contact between the microbial fermentation liquid and oxygen, resulting in the nematode-resistant microorganisms at the bottom of the nematode-resistant microbial inoculum being unable to contact oxygen. At the same time, when the existing device stirs, it is carried out by a stirring rod with stirring blades. Continuous stirring with a relatively large stirring rod will cause the contact area between the microorganisms and the stirring blades to be too large, resulting in mutual collisions, thereby damaging the integrity of microbial cells.
[0005] Therefore, a fermentation culture device for nematode-resistant microbial inoculum is proposed. Summary of the Invention
[0006] The purpose of the present invention is to provide a fermentation culture device for nematode-resistant microbial inoculum to solve the problems raised in the above background art.
[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a nematode-proof microbial agent fermentation and cultivation equipment, comprising a fermentation shell, the top of the fermentation shell is fixedly connected with a delivery pipe 1, the top of the fermentation shell is fixedly connected with a delivery pipe 2, the top of the fermentation shell is provided with an intermittent delivery component, the intermittent delivery component comprises a fixed frame fixedly connected to the top of the fermentation shell, the top lower surface of the fixed frame is rotatably connected with a rotating shell, the bottom of the rotating shell is symmetrically fixedly connected with a discharge pipe, and at least two discharge pipes are provided, a cylinder 1 is fixedly connected to the delivery pipe 2, the cylinder 1 is fixedly connected with a connecting pipe, a first motor is fixedly connected in the inner cavity of the cylinder 1, a rotating column 1 is fixedly connected to the output end of the first motor, a residual disc is fixedly connected to the top of the rotating column 1, a convex block is fixedly connected to the top of the rotating column 1, an arc gear is fixedly connected to the lower surface of the rotating shell, a shielding component is provided on the cylinder 1, the shielding component comprises an electric telescopic rod fixedly connected to the upper surface of the cylinder 1, and two shielding plates are fixedly connected to the telescopic shaft end of the electric telescopic rod.
[0008] Furthermore, a stirring assembly is provided on the fermentation shell, and the stirring assembly includes a rotating drum rotatably connected to the fermentation shell, a synchronous belt is transmission-connected between the outer side of the rotating drum and the outer side of the rotating column one, a second motor is fixedly connected to the top of the fixed frame, a rotating shaft is fixedly connected to the output end of the second motor, a first bevel gear is fixedly connected to the bottom of the rotating shaft, a plurality of cylinders two are fixedly connected to the outer annular array of the rotating shaft, and no less than four cylinders two are provided, an inner cavity of each of the cylinders two is fixedly connected to a fixing column, a threaded rod is rotatably connected to the middle part of each of the fixing columns, and a second bevel gear is fixedly connected to the side of each threaded rod close to the first bevel gear, and no less than two limit rods are fixedly connected to the inner cavity of each of the cylinders two, and a sliding block is slidably connected to the outer side of each two limit rods.
[0009] Furthermore, the stirring assembly also includes a connecting rod 1 fixedly connected to the side of each sliding block away from the second bevel gear, each connecting rod 1 is rotatably connected to a connecting rod 2 at one end away from the sliding block, each connecting rod 2 is rotatably connected to an end away from the connecting rod 1 at one end, each cylinder 2 is fixedly connected to a fixed plate in the inner cavity, each connecting rod 3 is rotatably connected to a plurality of connecting rods 4 at equal intervals on the lower surface, a plurality of connecting rods 4 are fixedly connected to a rotating column 2 at one end away from the connecting rod 3, and a plurality of rotating columns 2 are fixedly connected to a rotating block at the bottom.
[0010] Furthermore, an oxygenation component is provided on the cylinder two, and the oxygenation component includes a plurality of cylinders three fixedly connected to the outer side of the cylinder two, and the outer sides of the plurality of cylinders three are fixedly connected to no less than two air outlet pipes, and a suction pump is fixedly connected to the bottom upper surface of the fermentation shell.
[0011] Further, the connecting pipe is in a Y shape, the top of the connecting pipe is located on the movement path of the discharging pipe, the shape of the arc-shaped gear is set as a ring-shaped gear, the arc-shaped gear includes arc-shaped notches evenly and equidistantly arranged on the outer arc surface, straight grooves are evenly and equidistantly opened on the arc-shaped gear, the bottom of the connecting pipe is located on the movement paths of the two baffle plates, and the size of the baffle plate is adapted to the size of the connecting pipe.
[0012] Further, the residual disc is in a crescent shape and is composed of an outer arc surface and an inner concave arc surface. The outer arc surface of the residual disc is adapted to the arc-shaped notches of the arc-shaped gear, and the straight grooves of the arc-shaped gear are meshed with the convex blocks.
[0013] Further, the outer side of the rotating shaft is rotatably connected to the rotating cylinder, and the first bevel gear is meshed with the second cylinder.
[0014] Further, the sliding block is threadedly connected to the threaded rod, and the tops of the plurality of second rotating columns are rotatably connected to the fixing plate.
[0015] Further, the material of the rotating block is made of rubber material, and the second rotating column extends to the outside of the second cylinder.
[0016] Further, the two air outlet pipes are symmetrically arranged with reference to the center of the third cylinder, and the air outlet end of the suction pump is fixedly connected to the bottom of the rotating cylinder.
[0017] Compared with the prior art, the beneficial effects of the present invention are:
[0018] Through the setting of the intermittent component, it can supplement the carbon source in the nematode-proof microbial agent, and at the same time achieve the purpose of quantitative feeding, avoid the problem of opening the lid for feeding, and realize the purpose of automatic batch feeding. After the feeding, the staff has enough time to observe the concentration after feeding. At the same time, the intermittent feeding of the carbon source avoids the waste caused by one-time input, and avoids the inhibition of the growth of the nematode-proof microbial agent due to excessive carbon source.
[0019] Through the setting of the stirring component, multiple rotating blocks can swing reciprocally, so that the swinging of the multiple rotating blocks generates turbulence in the nematode-proof microbial agent, thereby improving the fluidity of the nematode-proof microbial agent, further increasing the full contact between the nematode-proof microbial agent and the nutrients. At the same time, when the multiple rotating blocks rotate 90 degrees to form a closed state, the contact area with the nematode-proof microbial agent increases, making the mixing more uniform. When the multiple rotating blocks are not closed, there are gaps between the rotating blocks, reducing the contact area, and effectively reducing the damage to the nematode-proof microorganisms during the stirring process.
[0020] The setting of the oxygenation component can provide sufficient oxygen supply for the nematode-proof microbial agent during the stirring process. At the same time, the air passage pipe directly leads oxygen into the nematode-proof microbial agent and nutrients, avoiding the situation of introducing oxygen into the existing reaction container, thereby reducing the occurrence that the nematode-proof microbial agent at the bottom of the nematode-proof microbial agent cannot contact oxygen, and avoiding the attachment of the nematode-proof microbial agent when the rotating block contacts the nematode-proof microbial agent. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 Stereoscopic schematic diagram of the overall structure of the present invention;
[0022] Figure 2 Cross-sectional schematic diagram of the fermentation housing structure of the present invention;
[0023] Figure 3 For the present invention Figure 2 Enlarged view of the structure at A in the present invention;
[0024] Figure 4 Stereoscopic schematic diagram of the intermittent feeding component structure of the present invention;
[0025] Figure 5 For the present invention Figure 4 Enlarged view of the structure at B in the present invention;
[0026] Figure 6 Stereoscopic schematic diagram of the structure of the second motor and the rotating shaft of the present invention;
[0027] Figure 7 Stereoscopic schematic diagram of the shielding component structure of the present invention;
[0028] Figure 8 Stereoscopic schematic diagram of the structure of the rotating cylinder and the second cylinder of the present invention;
[0029] Figure 9 Cross-sectional schematic diagram of the rotating cylinder structure of the present invention;
[0030] Figure 10 Stereoscopic schematic diagram of the structure of the second bevel gear, the threaded rod, the limiting rod and the sliding block of the present invention;
[0031] Figure 11 Stereoscopic schematic diagram of the structure of the third connecting rod and the fixing plate of the present invention;
[0032] Figure 12 For the present invention Figure 10 Enlarged view of the structure at C in the present invention;
[0033] Figure 13 Stereoscopic schematic diagram of the structure of the second rotating column and the rotating block of the present invention;
[0034] Figure 14 For the present invention Figure 12Enlarged view of the structure at position D in the [device];
[0035] Figure 15 For the present invention Figure 12 Enlarged view of the structure at position E in the [device].
[0036] In the figure:
[0037] 1. Fermentation shell; 2. Feeding pipe 1; 3. Feeding pipe 2;
[0038] The intermittent feeding assembly includes: 4. Fixed frame; 5. Rotating shell; 6. Discharge pipe; 7. Cylinder 1; 8. Connecting pipe; 9. First motor; 10. Rotating column 1; 11. Residual disc; 12. Convex block; 13. Arc gear;
[0039] The stirring assembly includes: 14. Rotating cylinder; 15. Synchronous belt; 16. Second motor; 17. Rotating shaft; 18. First bevel gear; 19. Cylinder 2; 20. Fixed column; 21. Second bevel gear; 22. Threaded rod; 23. Limiting rod; 24. Sliding block; 25. Connecting rod 1; 26. Connecting rod 2; 27. Connecting rod 3; 28. Fixed plate; 29. Connecting rod 4; 30. Rotating column 2; 31. Rotating block;
[0040] The oxygenation assembly includes: 32. Cylinder 3; 33. Air outlet pipe; 34. Suction pump;
[0041] The shielding assembly includes: 35. Electric telescopic rod; 36. Shielding plate. Detailed implementation manners
[0042] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0043] Please refer to Figures 1 to 14, an embodiment provided by the present invention: a fermentation and culture device for nematode-resistant microbial inoculum, comprising a fermentation housing 1. A first feeding pipe 2 is fixedly connected to the top of the fermentation housing 1. A rotatable and openable cover plate is provided at the top of the first feeding pipe 2. A second feeding pipe 3 is fixedly connected to the top of the fermentation housing 1. An intermittent feeding assembly is provided at the top of the fermentation housing 1. The intermittent feeding assembly includes a fixed frame 4 fixedly connected to the top of the fermentation housing 1. A rotating shell 5 is rotatably connected to the lower surface of the top of the fixed frame 4. The inside of the rotating shell 5 is filled with sucrose powder, which is manually put into the inside by the staff by opening the lid on the rotating shell 5. Symmetrically, a discharge pipe 6 is fixedly connected to the bottom of the rotating shell 5. There are no less than two discharge pipes 6. A first cylinder 7 is fixedly connected to the second feeding pipe 3, and the first cylinder 7 extends into the second feeding pipe 3. A communicating pipe 8 is fixedly connected to the first cylinder 7. The communicating pipe 8 is in a Y shape. The top of the communicating pipe 8 is located on the movement path of the discharge pipe 6, and the upper opening of the discharge pipe 6 is flush with the upper surface of the top of the first cylinder 7. A photoelectric sensor and a solenoid valve are fixedly installed on the outside of the discharge pipe 6, and the photoelectric sensor can control the opening and closing of the solenoid valve, so that when the discharge pipe 6 and the communicating pipe 8 are aligned with each other, the sucrose powder in the rotating shell 5 can be discharged into the communicating pipe 8 through the discharge pipe 6. A first motor 9 is fixedly connected to the inside of the first cylinder 7. The output end of the first motor 9 is fixedly connected to a first rotating column 10. A residual disk 11 is fixedly connected to the top of the first rotating column 10. The residual disk 11 is in a crescent shape and is composed of an outer arc surface and an inner concave arc surface. A convex block 12 is fixedly connected to the top of the first rotating column 10, and the convex block 12 is located at the eccentric position of the first rotating column 10. An arc-shaped gear 13 is fixedly connected to the lower surface of the rotating shell 5. The arc-shaped gear 13 is in the shape of an annular gear. A plurality of arc-shaped notches are evenly arranged on the outer arc surface of the arc-shaped gear 13. The arc-shaped notch of the arc-shaped gear 13 is adapted to the outer arc surface of the residual disk 11. A plurality of straight grooves are equidistantly arranged on the arc-shaped gear 13, and the arc-shaped notch of the arc-shaped gear 13 and the straight groove of the arc-shaped gear 13 are staggeredly distributed. The straight groove of the arc-shaped gear 13 cooperates with the convex block 12. After the outer arc surface of the residual disk 11 contacts the arc-shaped notch of the arc-shaped gear 13, at this time, the U-shaped groove of the arc-shaped gear 13 starts to mesh with the convex block 12. A shielding assembly is provided on the first cylinder 7. The shielding assembly includes an electric telescopic rod 35 fixedly connected to the upper surface of the first cylinder 7. The telescopic shaft end of the electric telescopic rod 35 is fixedly connected to two shielding plates 36. The bottom of the communicating pipe 8 is located on the movement path of the two shielding plates 36, and the size of the shielding plate 36 is adapted to the size of the communicating pipe 8.
[0044] A stirring assembly is provided on the fermentation housing 1. The stirring assembly includes a rotating cylinder 14 rotatably connected inside the fermentation housing 1. Synchronous pulleys are provided between the outer sides of the rotating cylinder 14 and the first rotating column 10, and a synchronous belt 15 is drivingly connected between the synchronous pulleys of the rotating cylinder 14 and the first rotating column 10. The synchronous belt 15 on one side of the first rotating column 10 is slidably connected in the hole of the first cylinder 7. The top of the fixed frame 4 is fixedly connected with a second motor 16. The output end of the second motor 16 is fixedly connected with a rotating shaft 17. The outer side of the rotating shaft 17 is rotatably connected to the rotating cylinder 14. The bottom of the rotating shaft 17 is fixedly connected with a first bevel gear 18. A plurality of second cylinders 19 are fixedly communicated in an annular array on the outer side of the rotating shaft 17. The first bevel gear 18 meshes with the second bevel gear 21. The number of the second cylinders 19 is not less than four. A fixed column 20 is fixedly connected to the inner cavity of each second cylinder 19. A threaded rod 22 is rotatably connected to the middle of each fixed column 20. A second bevel gear 21 is fixedly connected to one side of each threaded rod 22 close to the first bevel gear 18, and each second bevel gear 21 meshes with the first bevel gear 18. Not less than two limiting rods 23 are fixedly connected to the inner part of the inner cavity of each second cylinder 19. A sliding block 24 is slidably connected to the outer sides of every two limiting rods 23. The sliding block 24 is threadedly connected to the threaded rod 22.
[0045] The stirring assembly further includes a first connecting rod 25 fixedly connected to one side of each sliding block 24 away from the second bevel gear 21. One end of each first connecting rod 25 away from the sliding block 24 is rotatably connected to a second connecting rod 26. One end of each second connecting rod 26 away from the first connecting rod 25 is rotatably connected to a third connecting rod 27. A fixing plate 28 is fixedly connected to the inner cavity of each second cylinder 19. A plurality of fourth connecting rods 29 are rotatably connected to the lower surface of each third connecting rod 27 at equal intervals. One ends of the plurality of fourth connecting rods 29 away from the third connecting rod 27 are fixedly connected to a second rotating column 30. The tops of the plurality of second rotating columns 30 are rotatably connected to the fixing plate 28. The bottoms of the plurality of second rotating columns 30 are fixedly connected to a rotating block 31. The material of the rotating block 31 is made of rubber material. The second rotating column 30 extends to the outside of the second cylinder 19.
[0046] An oxygen increasing assembly is provided on the second cylinder 19. The oxygen increasing assembly includes a plurality of third cylinders 32 fixedly communicated with the outside of the second cylinder 19. Not less than two air outlet pipes 33 are fixedly communicated with the outside of the plurality of third cylinders 32. The two air outlet pipes 33 are symmetrically arranged with the center of the third cylinder 32 as a reference. The upper surface of the bottom of the fermentation housing 1 is fixedly connected with a suction pump 34. The suction pump 34 is externally connected with an oxygen cylinder. The air outlet end of the suction pump 34 is fixedly communicated with the bottom of the rotating cylinder 14.
[0047] The working principle of the above implementation is as follows:
[0048] The initialization steps are as follows:
[0049] The experimenter opens the cover plate on the first dosing pipe 2 and doses the nematode-resistant microbial agent into the inner cavity of the fermentation housing 1 through the inner cavity of the first dosing pipe 2. After the experimenter finishes dosing the nematode-resistant microbial agent, the cover plate on the first dosing pipe 2 is closed.
[0050] The working operation steps are as follows:
[0051] The working steps of the intermittent dosing assembly are as follows:
[0052] After the first motor 9 is powered on and starts, the output shaft end of the first motor 9 drives the first rotating column 10 to start rotating. The first rotating column 10 drives the residual disc 11 and the convex block 12 to rotate with the center of the first rotating column 10 as the reference. When the arc surface of the residual disc 11 passes through the arc notch of the arc-shaped gear 13, since the arc notch of the arc-shaped gear 13 and the outer arc surface of the residual disc 11 are mutually adapted, the rotation of the residual disc 11 will not drive the arc-shaped gear 13 to rotate. When the first rotating column 10 drives the convex block 12 to rotate to the arc-shaped gear 13, because the convex block 12 is located at the eccentric position of the first rotating column 10, when the convex block 12 approaches the arc-shaped gear 13, it will gradually enter the straight groove and mesh with each other. Therefore, the rotation of the convex block 12 drives the arc-shaped gear 13 to rotate with the connection point of the rotating housing 5 as the axis. At this time, the convex block 12 continues to rotate, so that the next straight groove of the arc-shaped gear 13 moves to the position of the previous straight groove. At the same time, the convex block 12 continues to rotate and slides out of the straight groove, so that the arc-shaped gear 13 rotates intermittently. At this time, the arc-shaped gear 13 drives the rotating housing 5 to rotate synchronously by sixty degrees. When the arc-shaped gear 13 rotates to drive the top nozzle of the discharge pipe 6 and the connecting pipe 8, because a photoelectric sensor and a solenoid valve are arranged on the outer side of the discharge pipe 6, and the photoelectric sensor can control the opening and closing of the solenoid valve, it plays a role that when the discharge pipe 6 rotates above the connecting pipe 8, the sucrose powder in the rotating housing 5 can be discharged from the discharge pipe 6 into the connecting pipe 8. Furthermore, the sucrose powder discharged from the rotating housing 5 enters the connecting pipe 8 through the discharge pipe 6. When the sucrose powder enters the connecting pipe 8, due to the influence of its own gravity, the sucrose powder enters the inner cavity of the fermentation housing 1 through the connecting pipe 8 and scatters in the nematode-resistant microbial agent. At the same time, when the sucrose powder dosing is completed and the staff observes that the appropriate concentration is reached after dosing, at this time, the electric telescopic rod 35 is powered on and its telescopic shaft end pushes the two shutter plates 36 towards the direction close to the top of the connecting pipe 8. When the two shutter plates 36 completely cover the top of the connecting pipe 8, at this time, the electric telescopic rod 35 stops extending. Therefore, the photoelectric sensor of the discharge pipe 6 cannot recognize that the discharge pipe 6 coincides with the connecting pipe 8, so the discharge pipe 6 stops feeding.
[0053] The setting of the intermittent component can supplement the carbon source in the nematode-proof microbial inoculum, achieve the purpose of quantitative dosing, avoid the problem of dosing with the lid open, and achieve the purpose of automatic batch dosing. It enables the staff to have enough time to observe the concentration after dosing. At the same time, the intermittent dosing of the carbon source avoids the waste caused by a one-time input and prevents the growth of the nematode-proof microbial inoculum from being inhibited due to excessive carbon source.
[0054] The working steps of the stirring component are as follows:
[0055] As described above, before the rotation column 1 starts to rotate, the rotating cylinder 14 remains stationary at this time. The experimenter turns on the second motor 16. After the second motor 16 is powered on, the output shaft end drives the rotating shaft 17 to start rotating in the inner cavity of the rotating cylinder 14, and the rotating shaft 17 rotates in the same direction as the rotating cylinder 14. The rotation of the rotating shaft 17 drives the first bevel gear 18 to rotate synchronously. Therefore, the first bevel gear 18 drives the second bevel gear 21 meshing with it to start rotating. The second bevel gear 21 drives the threaded rod 22 to rotate synchronously on the fixed column 20. The rotation of the threaded rod 22 drives the sliding block 24 to slide away from the second bevel gear 21 on the limiting rod 23. The sliding of the sliding block 24 drives the connecting rod 25 to move synchronously. The connecting rod 25 pushes the connecting rod 26 to move synchronously. The connecting rod 26 pushes the connecting rod 27 to move away from the threaded rod 22. The connecting rod 27 drives the connecting rod 29 to swing around the center of the rotation column 2 30 as the axis. As a result, the connecting rod 27 undergoes displacement. The function of the connecting rod 26 is to prevent the connecting rod 27 from getting stuck during displacement. At the same time, the connecting rod 29 drives the rotation column 2 30 to start rotating around its own center as the axis at the bottom of the fixing plate 28. Therefore, the rotation column 2 30 drives the rotating block 31 to start rotating by 90 degrees. Thus, multiple rotating blocks 31 rotate by 90 degrees synchronously to form a closure, thereby forming a complete plate shape. At the same time, when the staff drives the second motor 16 to reverse, the above opposite steps can be repeated at this time. Thus, the rotating block 31 can achieve reciprocating swinging, and at the same time, multiple rotating blocks 31 can also achieve synchronous rotation by 90 degrees to form a closure. When the rotation column 1 starts to rotate, at this time, the rotation column 1 drives the rotating cylinder 14 to rotate synchronously through the synchronous belt 15. Therefore, the rotating cylinder 14 drives the four cylinders 19 to rotate around the center of the rotating cylinder 14. Thus, the four cylinders 19 and the rotating block 31 that can achieve reciprocating swinging together play a role in stirring.
[0056] The setting of the stirring component enables the multiple rotating blocks 31 to swing reciprocally. Thus, the swinging of the multiple rotating blocks 31 creates turbulence in the nematode-proof microbial agent, thereby improving the fluidity of the nematode-proof microbial agent, further increasing the sufficient contact between the nematode-proof microbial agent and nutrients. At the same time, when the multiple rotating blocks 31 rotate by 90 degrees to form a closed state, a complete board is formed, and the contact area with the nematode-proof microbial agent increases, making the mixing better and more uniform. When the multiple rotating blocks 31 are not closed, there are gaps between the rotating blocks 31, reducing the contact area and effectively minimizing the damage to the nematode-proof microorganism during the stirring process.
[0057] The working steps of the oxygenation component are as follows:
[0058] The experimenter turns on the switch of the suction pump 34, so that the suction pump 34 is powered on and starts to work. The suction pump 34 sucks the oxygen in the external oxygen cylinder into the inner cavity of the rotary drum 14. Since oxygen is a gas with fluidity, the oxygen enters the inner cavity of the second cylinder 19 through the rotary drum 14, and then enters the inner cavity of the third cylinder 32 through the second cylinder 19. Finally, the oxygen enters the inner cavity of the fermentation shell 1 through the air outlet pipe 33 and comes into contact with the nematode-proof microbial agent. At the same time, the air outlet pipe 33 blows the oxygen to the side of the rotating block 31, and after the fermentation of the nematode-proof microorganism is completed.
[0059] The setting of the oxygenation component can provide sufficient oxygen supply for the nematode-proof microbial agent during the stirring process. At the same time, the air outlet pipe 33 directly passes oxygen into the nematode-proof microbial agent and nutrients, avoiding the situation of passing oxygen into the reaction container used currently, thereby reducing the occurrence that the nematode-proof microorganisms at the bottom of the nematode-proof microbial agent cannot contact oxygen, and avoiding the attachment of nematode-proof microorganisms on the rotating block 31 when it contacts the nematode-proof microbial agent.
[0060] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. Without more limitations, an element defined by the statement "including a..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.
[0061] Although embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A nematode-proof microbial agent fermentation and cultivation device, comprising a fermentation shell (1), characterized in that: The top of the fermentation shell (1) is fixedly connected to a delivery pipe 1 (2), the top of the fermentation shell (1) is fixedly connected to a delivery pipe 2 (3), the top of the fermentation shell (1) is provided with an intermittent delivery component, the intermittent delivery component comprises a fixed frame (4) fixedly connected to the top of the fermentation shell (1), the top lower surface of the fixed frame (4) is rotatably connected to a rotating shell (5), the bottom of the rotating shell (5) is symmetrically fixedly connected to a discharge pipe (6), and at least two of the discharge pipes (6) are provided, the delivery pipe 2 (3) is fixedly connected to a cylinder 1 (7), and the cylinder 1 (7) is fixedly connected to a connecting pipe The first motor (9) is fixedly connected to the inner cavity of the cylinder (7), the output end of the first motor (9) is fixedly connected to a rotating column (10), the top of the rotating column (10) is fixedly connected to a residual disc (11), the top of the rotating column (10) is fixedly connected to a convex block (12), the lower surface of the rotating shell (5) is fixedly connected to an arc gear (13), and a shielding assembly is provided on the cylinder (7), the shielding assembly includes an electric telescopic rod (35) fixedly connected to the upper surface of the cylinder (7), and the telescopic shaft end of the electric telescopic rod (35) is fixedly connected to two shielding plates (36); The communicating pipe (8) is Y-shaped, the top of the communicating pipe (8) is located on the movement path of the discharge pipe (6), the shape of the arc gear (13) is set to be a ring-shaped gear, the arc gear (13) includes arc notches evenly and equidistantly opened on the outer arc surface, straight grooves evenly and equidistantly opened on the arc gear (13), the bottom of the communicating pipe (8) is located on the movement path of the two shielding plates (36), and the size of the shielding plates (36) is adapted to the size of the communicating pipe (8); The residual circular disc (11) is in a crescent shape and is composed of an outer arc surface and an inner concave arc surface. The outer arc surface of the residual circular disc (11) is adapted to the arc-shaped notch of the arc-shaped gear (13), and the straight groove of the arc-shaped gear (13) is meshed with the protrusion (12). A photoelectric sensor and a solenoid valve are arranged on the outside of the discharge pipe (6), and the photoelectric sensor can control the opening and closing of the solenoid valve, so that when the discharge pipe (6) rotates to the top of the connecting pipe (8), the sucrose powder in the rotating shell (5) can be discharged from the discharge pipe (6) into the connecting pipe (8), and then the sucrose powder discharged from the rotating shell (5) enters the connecting pipe (8) through the discharge pipe (6). When the sucrose powder enters the connecting pipe (8), due to the influence of its own gravity, the sucrose powder enters the discharge pipe (6) through the connecting pipe (8). The inner cavity of the fermentation shell (1) is filled with sucrose powder and scattered in the anti-nematode microbial agent. At the same time, the sucrose powder is added. After the staff observes that the addition reaches an appropriate concentration, the electric telescopic rod (35) is energized and its telescopic shaft end pushes the two shielding plates (36) to move toward the top of the connecting pipe (8). When the two shielding plates (36) completely cover the top of the connecting pipe (8), the electric telescopic rod (35) stops extending. Therefore, the photoelectric sensor of the discharge pipe (6) cannot recognize that the discharge pipe (6) overlaps with the connecting pipe (8), so that the discharge pipe (6) stops unloading.
2. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 1, characterized in that: The fermentation shell (1) is provided with a stirring assembly, the stirring assembly comprising a rotating drum (14) rotatably connected to the fermentation shell (1), a synchronous belt (15) is transmission-connected between the outer side of the rotating drum (14) and the outer side of the rotating column (10), a second motor (16) is fixedly connected to the top of the fixed frame (4), a rotating shaft (17) is fixedly connected to the output end of the second motor (16), a first bevel gear (18) is fixedly connected to the bottom of the rotating shaft (17), and the outer annular array of the rotating shaft (17) is fixedly connected to the outer ring of the rotating shaft (17). There are a plurality of cylinders (19), and at least four of the cylinders (19) are provided. The inner cavity of each cylinder (19) is fixedly connected to a fixing column (20), and the middle of each fixing column (20) is rotatably connected to a threaded rod (22). The side of each threaded rod (22) close to the first bevel gear (18) is fixedly connected to a second bevel gear (21). The inner cavity of each cylinder (19) is fixedly connected to at least two limiting rods (23), and the outer sides of each of the two limiting rods (23) are slidably connected to a sliding block (24).
3. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 2, characterized in that: The stirring assembly also includes a connecting rod one (25) fixedly connected to a side of each sliding block (24) away from the second bevel gear (21); the end of each connecting rod one (25) away from the sliding block (24) is rotatably connected to a connecting rod two (26); the end of each connecting rod two (26) away from the connecting rod one (25) is rotatably connected to a connecting rod three (27); the inner cavity of each cylinder two (19) is fixedly connected to a fixed plate (28); the lower surface of each connecting rod three (27) is rotatably connected to a plurality of connecting rods four (29) at equal intervals; the ends of the plurality of connecting rods four (29) away from the connecting rod three (27) are fixedly connected to a rotating column two (30); the bottoms of the plurality of rotating columns two (30) are fixedly connected to a rotating block (31).
4. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 2, characterized in that: The cylinder two (19) is provided with an oxygenation assembly, which includes a plurality of cylinder threes (32) fixedly connected to the outside of the cylinder two (19), and the outsides of the plurality of cylinder threes (32) are fixedly connected to at least two air outlet pipes (33), and a suction pump (34) is fixedly connected to the bottom upper surface of the fermentation shell (1).
5. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 2, characterized in that: The outer side of the rotating shaft (17) is rotatably connected to the rotating drum (14), and the first bevel gear (18) and the second drum (19) are meshed with each other.
6. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 3, characterized in that: The sliding block (24) is threadedly connected to the threaded rod (22), and the tops of the plurality of rotating columns (30) are rotatably connected to the fixed plate (28).
7. The anti-nematode microbial agent fermentation and cultivation equipment according to claim 3, characterized in that: The rotating block (31) is made of rubber material, and the second rotating column (30) extends to the outside of the second cylinder (19).
8. The fermentation and cultivation equipment for anti-nematode microbial agent according to claim 4, characterized in that: The two air outlet pipes (33) are symmetrically arranged with the center of the cylinder (32) as a reference, and the air outlet end of the suction pump (34) is fixedly connected to the bottom of the rotating drum (14).
Citation Information
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