Cantilever shaft stirring type disposable bioreactor

Abstract

According to the cantilever shaft stirring type disposable bioreactor, a stirring device is composed of a motor, a planetary reducer, a mounting base, a magnetic driving assembly, a fixing base, an inner magnetic assembly, a stirring shaft and at least one paddle which are arranged from top to bottom, and the motor, the planetary reducer, the mounting base and the magnetic driving assembly are arranged on the outer side of a flexible container; the inner magnetic assembly, the stirring shaft and the at least one paddle are arranged in the flexible container, the inner magnetic assembly and the stirring shaft form a cantilever shaft structure, the fixed seat is connected with the flexible container in a welding manner, the fixed seat is connected with the magnetic driving assembly through a hoop, and the inner magnetic assembly is fixed on a protruding shaft part of the fixed seat through a first bearing and a second bearing. The stirring device is simple in structure and easy to machine and weld, all assemblies of the stirring device and the flexible container can be centered conveniently, the rotation concentricity of the stirring device is improved, and the jumping problem of rotation of the cantilever shaft is effectively reduced.

Description

Technical Field

[0001] The present application relates to the technical field of bioreactor equipment, and in particular to a cantilever shaft stirring type disposable bioreactor. Background Art

[0002] A disposable bioreactor is a bioreactor that uses a disposable container instead of a culture container made of stainless steel or glass, and usually includes a disposable stirring device and a disposable flexible container. In the prior art, the stirring device in the disposable bioreactor usually adopts a single-span shaft structure supported by upper and lower bearings. This single-span shaft structure is fixed to the top and bottom of the disposable flexible container by upper and lower bearings, respectively. The structure is complex and difficult to process and weld. It is difficult to align the upper and lower bearing fixing seats and the drive assembly, and it is difficult for the stirring device to obtain satisfactory rotation concentricity. In addition, the stirring device in this disposable bioreactor can also use a cantilever shaft structure, but the bearings in this cantilever shaft structure are fixedly connected by threads, and it is very easy for the threads to loosen when the stirring shaft rotates and stops, resulting in problems such as eccentric rotation and excessive jitter of the magnetically driven internal magnetic assembly. Therefore, an improved technical solution is urgently needed to solve the above problems. Summary of the invention

[0003] In view of this, the present application proposes a cantilever shaft stirring type disposable bioreactor, comprising a flexible container, a rigid container supporting the flexible container, and a stirring device; the stirring device comprises a motor, a planetary reducer, a mounting seat, a magnetic drive component, a fixing seat, an internal magnetic component, a stirring shaft and at least one paddle arranged from top to bottom, and the stirring device is fixed to the rigid container through a cantilever;

[0004] The motor, the planetary reducer, the mounting seat, and the magnetic drive assembly are arranged outside the flexible container, the inner magnetic assembly, the stirring shaft, and at least one blade are arranged inside the flexible container, and the inner magnetic assembly and the stirring shaft form a cantilever shaft structure;

[0005] The fixing seat is connected to the flexible container by welding, and the fixing seat is connected to the magnetic drive component by a clamp; the internal magnetic component includes a first bearing and a second bearing, and the internal magnetic component is fixed to the protruding shaft part of the fixing seat by the first bearing and the second bearing.

[0006] In an optional embodiment, the protruding shaft portion of the fixing seat is formed in an integral manner at the lower end portion of the fixing seat.

[0007] In an optional embodiment, the upper end portion of the fixing seat has a concave structure, and the concave structure is used to accommodate the magnetic drive component.

[0008] In an optional embodiment, the first bearing is fixed to the first end of the protruding shaft portion of the fixing seat connected to the upper end portion of the fixing seat, and the second bearing is fixed to the second end of the protruding shaft portion of the fixing seat located at the top, and the outer diameter of the first end is larger than the outer diameter of the second end.

[0009] In an optional embodiment, the top of the second end has a cross-cut structure, the upper diameter of the cross-cut structure is larger than the lower diameter, and is used to prevent the second bearing from falling off when the second bearing is fixed to the second end.

[0010] In an optional embodiment, a tension pin is provided on the inner side of the cross-cut structure to prevent the cross-cut structure from shrinking when the second bearing is fixed to the second end.

[0011] In an optional embodiment, the internal magnetic assembly includes a bearing seat for accommodating the first bearing and the second bearing, and the lower end of the bearing seat is provided with a groove for accommodating the shaft head portion of the stirring shaft, and the shaft head portion and the groove have a mating square-circle structure.

[0012] In an optional embodiment, the stirring shaft has a stirring shaft head boss below the shaft head portion, and the lower end of the bearing seat is provided with at least a first snap-fit ​​component and a second snap-fit ​​component for fixing and positioning the stirring shaft head boss.

[0013] In an optional embodiment, the stirring shaft has a positioning hole for fixing the at least one paddle.

[0014] In an optional embodiment, the fixing seat, the magnetic drive assembly and the flexible container are centrally arranged, and the fixing seat is welded to the top axial center position of the flexible container.

[0015] The embodiments of the present application can at least achieve the following beneficial effects:

[0016] The cantilever shaft stirring type disposable bioreactor of the embodiment of the present application is a stirring device composed of a motor, a planetary reducer, a mounting seat, a magnetic drive assembly, a fixed seat, an internal magnetic assembly, a stirring shaft and at least one blade arranged from top to bottom, wherein the motor, the planetary reducer, the mounting seat, and the magnetic drive assembly are arranged on the outside of the flexible container, the internal magnetic assembly, the stirring shaft, and at least one blade are arranged inside the flexible container, the internal magnetic assembly and the stirring shaft constitute a cantilever shaft structure, and the fixed seat is connected to the flexible container by welding, the fixed seat is connected to the magnetic drive assembly by a clamp, and the internal magnetic assembly is fixed to the protruding shaft portion of the fixed seat by a first bearing and a second bearing. Therefore, the embodiment of the present application has a simple structure and is easy to process and weld, which is convenient for the centering arrangement between the components of the stirring device and the flexible container, improves the rotation concentricity of the stirring device, and effectively reduces the beating problem of the rotation of the cantilever shaft. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The present application will be more fully understood through the following detailed description in conjunction with the accompanying drawings, in which:

[0018] Figure 1 is a schematic structural diagram of a cantilever shaft stirring disposable bioreactor according to an embodiment of the present application;

[0019] Figure 2 is a schematic structural diagram of an internal magnetic assembly according to an embodiment of the present application;

[0020] Figure 3 is a schematic structural diagram of a fixing base according to an embodiment of the present application;

[0021] Figure 4 is a structural schematic diagram of a bearing seat according to an embodiment of the present application;

[0022] Figure 5 is a schematic structural diagram of a stirring shaft according to an embodiment of the present application;

[0023] Figure 6 is a schematic structural diagram of a blade according to an embodiment of the present application;

[0024] Figure 7 It is a structural schematic diagram of a magnetic drive assembly according to an embodiment of the present application.

[0025] Reference numerals:

[0026] a-cantilever shaft stirring disposable bioreactor; b-stirring device; c-medium; 1-motor; 2-planetary reducer; 3-mounting seat; 4-clamp; 5-fixed seat; 6-internal magnetic assembly; 7-stirring shaft; 8-paddle; 9-medium discharge port; 10-ventilation assembly; 11-cantilever; 12-magnetic drive assembly; 13-medium inlet; 14-flexible container; 15-rigid container; 16-first bearing; 17-internal magnet; 18-bearing seat; 19-second bearing; 20-tightening pin; 5a- Upper opening portion; 5b-concave bottom plate; 5c-fixed seat edge; 5d-protruding shaft portion; 5e-second end; 5f-top of the second end; 5g-cross cut structure; 5h-first end; 18b-first snap-fit ​​component; 18c-groove; 18d-second snap-fit ​​component; 7a-shaft head portion; 7b-positioning hole; 7c-stirring shaft head boss; 8a-blade; 8b-hub; 8c-second screw; 8d-first screw; 8e-mortise and tenon structure; 12a-driving magnet; 12b-driving magnet seat. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings of the embodiments of the present application. It should be understood that the described embodiments are only some exemplary embodiments of the present application, rather than all embodiments, and therefore the following detailed description of the embodiments of the present application is not intended to limit the scope of protection claimed in the present application. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

[0028] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood by specific circumstances.

[0029] In this application, the terms "front", "back", "left", "right", "up", "down", "center", "vertical", "horizontal", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, or the positions or positional relationships in which the devices or components are usually placed when in use. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting this application. In addition, the terms "first", "second", "third", "fourth", etc. are only used to distinguish descriptions, and cannot be understood as indicating or implying relative importance.

[0030] Figure 1 Schematic diagram of the structure of a cantilever shaft stirring disposable bioreactor according to an embodiment of the present application. Figure 1 As shown, the cantilever shaft stirring type disposable bioreactor a comprises a flexible container 14, a rigid container 15 supporting the flexible container 14, and a stirring device b. The stirring device b comprises a motor 1, a planetary reducer 2, a mounting seat 3, a magnetic drive assembly 12, a fixing seat 5, an internal magnetic assembly 6, a stirring shaft 7, and at least one paddle 8 arranged from top to bottom, and the stirring device b is fixed to the rigid container 15 through a cantilever 11.

[0031] In this embodiment, the motor 1, the planetary reducer 2, the mounting seat 3, and the magnetic drive assembly 12 are provided as the outer part of the stirring device b on the outer side of the flexible container 14; the inner magnetic assembly 6, the stirring shaft 7, and at least one blade 8 are provided as the inner part of the stirring device b on the inside of the flexible container 14. The inner magnetic assembly 6 and the stirring shaft 7 form a cantilever shaft structure, which can solve the problem of assembling and centering two bearings caused by the traditional single-span shaft structure supported by upper and lower bearings.

[0032] The fixing seat 5 is connected to the flexible container 14 by welding, and the fixing seat 5 is connected to the magnetic drive assembly 12 by a clamp 4 .

[0033] In this embodiment, the flexible container 14 is used to hold the medium c, which can be used for stem cell culture, and the rigid container 15 is used to support the flexible container 14 and provide it with heating and cooling control required for the process. In one embodiment, the flexible container 14 is made of a flexible plastic material, which can be spliced ​​from one or more pieces of flexible plastic, produced in a controlled environment, and sterilized by irradiation to ensure that the interior is sterile.

[0034] In one embodiment, if Figure 1As shown. A medium inlet 13 may be provided at the top of the flexible container 14, through which a medium c, such as gas or liquid, may be introduced into the flexible container 14. A medium outlet 9 may be provided at the bottom of the flexible container 14, for discharging all or part of the medium c after the process is completed. An independent ventilation component 10 may also be provided at the bottom of the flexible container 14, and the ventilation component 10 may be directly welded to the flexible container 14, and is not directly or indirectly connected to the stirring shaft 7.

[0035] In one embodiment, the fixing seat 5, the magnetic drive assembly 12 and the flexible container 14 are arranged in a central position, thereby avoiding abnormal stirring caused by the bending of the shaft of the stirring device b, reducing bearing wear and increasing service life.

[0036] In one embodiment, the stirring device b is fixed to the upper side of the rigid container 15 via a cantilever 11, and the cantilever 11 may be provided with at least one fixed support point. In a preferred embodiment, the at least one fixed support point may include 1 to 6 fixed support points.

[0037] In this embodiment, Figure 2 As shown, the inner magnetic assembly 6 may include a first bearing 16 and a second bearing 19, and the inner magnetic assembly 6 is fixed to the protruding shaft portion 5d of the fixing seat 5 through the first bearing 16 and the second bearing 19. In this structure, the first bearing 16 and the second bearing 19 are both located at the upper part of the inner part of the stirring device b, which is different from the single-span shaft structure supported by upper and lower bearings in the prior art. The structure is simpler and the difficulty of processing and welding is small. The first bearing 16 and the second bearing 19, the fixing seat 5, the magnetic drive assembly 12 and the flexible container 14 are easily aligned, so that the stirring device b can obtain satisfactory rotation concentricity.

[0038] In one embodiment, the first bearing 16 and the second bearing 19 may be stainless steel or ceramic deep groove ball bearings, the two bearings are of different sizes, and are coaxially arranged, and the distance between the two bearings can be adjusted according to the volume size of the flexible container 14. In other embodiments, the first bearing 16 and the second bearing 19 may also be plastic bearings or other types of bearings.

[0039] In one embodiment, the fixing seat 5 is welded to the top axial center of the flexible container 14 to isolate the magnetic drive assembly 12 from the internal magnetic assembly 6. In one embodiment, the fixing seat 5 can be fixed to the top axial center of the flexible container 14 by hot-melting or other hot-welding methods the edge 5c of the fixing seat.

[0040] In one embodiment, if Figure 2As shown, the protruding shaft portion 5d of the fixing seat 5 is formed in an integral manner at the lower end portion of the fixing seat 5. The protruding shaft portion 5d of the fixing seat 5 is integrally formed with the fixing seat 5, and is used to fix the first bearing 16 and the second bearing 19 of the internal magnetic component 6. The integral molding can be injection molding or machining to ensure the overall coaxiality of the fixing seat 5. This structure is different from other traditional fixing methods such as threads, and can solve the problem of non-concentric rotation of the internal magnetic component 6 caused by loosening of other fixing methods such as threads, reduce the rotation jump of the internal magnetic component 6, ensure the strength and normal operation of the stirring device b, and also realize the clockwise or counterclockwise rotation of the stirring shaft 7.

[0041] In one embodiment, if Figure 2 As shown, the upper end portion of the fixing seat 5 has a concave structure, and the concave structure is used to accommodate the magnetic drive assembly 12. The upper opening portion 5a of the concave structure is connected to the magnetic drive assembly 12 through a clamp 4, and the clamp 4 can be a two-stage or three-stage type. After the connection, the lower end of the driving magnet assembly 12 is kept at a gap of 1 to 3 mm from the concave bottom plate 5b of the concave structure. Preferably, the gap is kept at 1 mm. Since the cantilever shaft structure of the stirring device b does not need to set a bearing support between the stirring shaft 7 and the bottom of the flexible container 14, it is easier to dock the upper opening portion 5a of the concave structure with the magnetic drive assembly 12.

[0042] In one embodiment, if Figure 2 and 3 As shown, the first bearing 16 is fixed to the first end 5h of the protruding shaft portion 5d of the fixing seat 5 connected to the upper end portion of the fixing seat 5, and the second bearing 19 is fixed to the second end 5e of the protruding shaft portion 5d of the fixing seat 5 located at the top, and the outer diameter of the first end 5h is greater than the outer diameter of the second end 5e.

[0043] In one embodiment, the top 5f of the second end 5e has a cross-cut structure 5g, the upper diameter of the cross-cut structure 5g is larger than the lower diameter, and is used to prevent the second bearing 19 from falling off when the second bearing 19 is fixed to the second end 5e. The inner hole of the second bearing 19 is smaller than the outer diameter of the top 5f of the second end 5e. When the first bearing 16 and the second bearing 19 are installed and fixed to the protruding shaft portion 5d of the fixing seat 5, under the action of external force, the cross-cut structure 5g of the top 5f of the second end 5e shrinks inward, the first bearing 16 reaches the top step of the first end 5h of the protruding shaft portion 5d of the fixing seat, and the second bearing 19 reaches the second end 5e of the protruding shaft portion of the fixing seat 5. The external force acting on the top 5f of the second end 5e is released, and the cross-cut structure 5g rebounds, which can clamp the second bearing 19 and prevent the second bearing 19 from falling off.

[0044] In one embodiment, a tension pin 20 is disposed inside the cross-cut structure 5g to prevent the cross-cut structure 5g from shrinking under an abnormal external force when the second bearing 19 is fixed to the second end 5e.

[0045] In one embodiment, if Figure 2 As shown, the internal magnetic assembly 6 includes a bearing seat 18 for accommodating the first bearing 16 and the second bearing 19, and the outer rings of the first bearing 16 and the second bearing 19 are interference fit with the bearing seat 18. The internal magnetic assembly 6 also includes an internal magnet 17 disposed in the bearing seat 18, and the internal magnet 17 and the first bearing 16 are located in the same cross section, wherein the first bearing 16 is located at the center, and the internal magnet 17 is evenly arranged in a fixed center circle in the outer disk of the bearing seat 18.

[0046] In one embodiment, if Figure 7 As shown, the magnetic drive assembly 12 includes a driving magnet 12a and a driving magnet seat 12b. The driving magnet 12a and the inner magnet 17 can adopt six groups of cylindrical magnets to axially conduct the magnetic torque in a contactless manner. The stirring device b drives the magnetic drive assembly 12 to rotate through the output shaft of the motor 1 and the output shaft of the planetary reducer 2. The magnetic drive assembly 12 drives the inner magnet 17 through the driving magnet 12a to drive the inner magnetic assembly 6 to move synchronously, so that the inner magnetic assembly can rotate clockwise or counterclockwise.

[0047] In one embodiment, if Figure 4 As shown, the lower end of the bearing seat 18 is provided with a groove 18c which can accommodate the shaft head portion 7a of the stirring shaft 7, and the shaft head portion 7a and the groove have a square-sectioned circular structure for docking. This square-sectioned circular structure facilitates the docking of the shaft head portion 7a and the groove, and can also prevent the stirring shaft 7 from rotating itself, and plays a role in transmitting torque.

[0048] In one embodiment, if Figure 4 and 5 As shown, the stirring shaft 7 has a stirring shaft head boss 7c below the shaft head portion 7a, and the lower end of the bearing seat is provided with at least one first snap-fit ​​component 18b and a second snap-fit ​​component 18d for fixing and positioning the stirring shaft head boss 7c. The first snap-fit ​​component 18b is used to ensure the axial positioning and locking of the stirring shaft head boss 7c, and the second snap-fit ​​component 18d is used to ensure the radial positioning of the stirring shaft head boss 7c. The first snap-fit ​​component 18b and the second snap-fit ​​component 18d are located in the same cross section, and are arranged in 4 to 6 sections at alternating intervals. Preferably, 4 sections are arranged at alternating intervals. The inner dimension of the second snap-fit ​​component 18d is smaller than the outer dimension of the boss 7c, and the second snap-fit ​​component 18d is installed in cooperation with the stirring shaft head boss 7c by means of plastic elastic deformation.

[0049] In one embodiment, if Figure 5 and 6 As shown, the stirring shaft 7 has at least one positioning hole 7b for fixing the at least one paddle 8. The at least one paddle 8 can be fixed to the at least one positioning hole 7b of the stirring shaft 7 by a first screw 8d, and the paddle 8 can be adjusted up and down. The paddle 8 can include a hub 8b and a group of blades 8a, and the blade 8s is installed on the hub 8b, and the hub 8b is locked by a mortise and tenon structure 8e and a second screw 8c. The first screw 8d and the second screw 8c can be stainless steel or plastic screws.

[0050] The cantilever shaft stirring type disposable bioreactor of the embodiment of the present application is a stirring device composed of a motor, a planetary reducer, a mounting seat, a magnetic drive assembly, a fixed seat, an internal magnetic assembly, a stirring shaft and at least one blade arranged from top to bottom, wherein the motor, the planetary reducer, the mounting seat, and the magnetic drive assembly are arranged on the outside of the flexible container, the internal magnetic assembly, the stirring shaft, and at least one blade are arranged inside the flexible container, the internal magnetic assembly and the stirring shaft constitute a cantilever shaft structure, and the fixed seat is connected to the flexible container by welding, the fixed seat is connected to the magnetic drive assembly by a clamp, and the internal magnetic assembly is fixed to the protruding shaft portion of the fixed seat by a first bearing and a second bearing. Therefore, the embodiment of the present application has a simple structure and is easy to process and weld, which is convenient for the centering arrangement between the components of the stirring device and the flexible container, improves the rotation concentricity of the stirring device, and effectively reduces the beating problem of the rotation of the cantilever shaft.

[0051] The implementation methods of the present application are not limited to the above-mentioned embodiments. Without departing from the spirit and scope of the present application, ordinary technicians in this field can make various changes and improvements to the present application in form and details, and these are considered to fall within the scope of protection of the present application.

Claims

1. A cantilever shaft stirred disposable bioreactor, characterized in that: The invention comprises a flexible container, a rigid container supporting the flexible container, and a stirring device; the stirring device comprises a motor, a planetary reducer, a mounting seat, a magnetic drive assembly, a fixing seat, an internal magnetic assembly, a stirring shaft and at least one paddle arranged from top to bottom, and the stirring device is fixed to the rigid container through a cantilever; The motor, the planetary reducer, the mounting seat, and the magnetic drive assembly are arranged outside the flexible container, the inner magnetic assembly, the stirring shaft, and at least one blade are arranged inside the flexible container, and the inner magnetic assembly and the stirring shaft form a cantilever shaft structure; The fixing seat is connected to the flexible container by welding, and the fixing seat is connected to the magnetic drive component by a clamp; the internal magnetic component includes a first bearing and a second bearing, and the internal magnetic component is fixed to the protruding shaft part of the fixing seat by the first bearing and the second bearing.

2. The cantilever shaft stirred disposable bioreactor according to claim 1, characterized in that: The protruding shaft portion of the fixing seat is formed at the lower end portion of the fixing seat in an integrally formed manner.

3. The cantilever shaft stirred disposable bioreactor according to claim 2, characterized in that: The upper end portion of the fixing seat has a concave structure, and the concave structure is used to accommodate the magnetic drive component.

4. The cantilever shaft stirred disposable bioreactor according to claim 3, characterized in that: The first bearing is fixed to the first end of the protruding shaft portion of the fixing seat connected to the upper end portion of the fixing seat, and the second bearing is fixed to the second end of the protruding shaft portion of the fixing seat located at the top, and the outer diameter of the first end is greater than the outer diameter of the second end.

5. The cantilever shaft stirred disposable bioreactor according to claim 4, characterized in that: The top of the second end has a cross cut structure, the upper diameter of the cross cut structure is larger than the lower diameter, and is used to prevent the second bearing from falling off when the second bearing is fixed to the second end.

6. The cantilever shaft stirred disposable bioreactor according to claim 5, characterized in that: An expansion pin is provided on the inner side of the cross-cut structure, which is used to prevent the cross-cut structure from shrinking when the second bearing is fixed to the second end.

7. The cantilever shaft stirred disposable bioreactor according to any one of claims 1 to 6, characterized in that: The inner magnetic assembly includes a bearing seat for accommodating the first bearing and the second bearing. The lower end of the bearing seat is provided with a groove for accommodating the shaft head portion of the stirring shaft. The shaft head portion and the groove have a square-circle structure that fits in a butt joint.

8. The cantilever shaft stirred disposable bioreactor according to claim 7, characterized in that: The stirring shaft has a stirring shaft head boss below the shaft head portion, and the lower end of the bearing seat is provided with at least a first snap-fit ​​component and a second snap-fit ​​component for fixing and positioning the stirring shaft head boss.

9. The cantilever shaft stirred disposable bioreactor according to claim 8, characterized in that: The stirring shaft has a positioning hole for fixing the at least one paddle.

10. The cantilever shaft stirred disposable bioreactor according to claim 9, characterized in that: The fixing seat, the magnetic drive assembly and the flexible container are centrally arranged, and the fixing seat is welded to the top axial center position of the flexible container.

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