Cell culture fluid oscillating mechanism for gene detection

Abstract

The invention discloses a cell culture fluid oscillation mechanism for gene detection, and relates to the technical field of cell culture.The cell culture fluid oscillation mechanism comprises a bottom shell, a through hole is formed in the middle of a top plate, an annular groove coaxial with the through hole is formed in the inner side wall of the through hole, and an oscillation table is slidably arranged on the inner side of the annular groove; wherein a clamping mechanism for clamping a culture dish is arranged at the top of the oscillation table; wherein a cross-shaped pushing mechanism is arranged in the bottom shell, and the oscillation table achieves cross-shaped oscillation through the cross-shaped pushing mechanism. The whole device can perform two different oscillation modes through the cross-shaped pushing mechanism and the eccentric shaking mechanism to adapt to different culture conditions, if a culture solution is thin, cross-shaped oscillation is used, namely, the cross-shaped pushing mechanism performs longitudinal sliding and transverse sliding of the oscillation table separately, so that cross-shaped oscillation is realized; when the culture solution is thick, the whole oscillation table rotates eccentrically by using eccentric oscillation, namely an eccentric shaking mechanism, so that the application range of the whole device is widened.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a cell culture fluid oscillating mechanism for gene detection. Background Art

[0002] Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, we can not only obtain a large number of cells, but also study cell signal transduction, cell anabolism, cell growth and proliferation, etc.

[0003] After searching, a Chinese patent with announcement number CN220665346U discloses a multi-track oscillation device for cell culture, including a main body of the oscillation device, a shaking component for lateral oscillation of the cell culture carrier, and a pushing component for longitudinal oscillation of the cell culture carrier, wherein the shaking component includes a fixed frame arranged on the top of the main body for placement, a sliding frame arranged on the inner side of the fixed frame for oscillation, and three pushing frames arranged evenly on both sides of the sliding frame for squeezing. The patent uses a main body, a clamping plate, a pull rod, a cam and a push plate. After the end of the cam is separated from the sliding frame, the first spring extends to squeeze the push plate, thereby solving the problem that the existing equipment cannot oscillate the culture dish in multiple directions, which easily leads to uneven distribution of nutrients in the culture solution, reduces the growth rate of cell culture, and cannot conveniently clamp and fix the cell culture carrier stably, affecting the effect and practicality of the equipment in oscillating the cell culture solution.

[0004] However, the above invention has the following shortcomings: cell culture requires the addition of culture drugs for culture. If the drugs are relatively diluted, the vibration method generally used is the cross-stroke method (similar to the above device). In this way, after ensuring that the cells can be fully mixed with the culture drugs, most of the cells are distributed in the middle area, which is convenient for subsequent sampling and observation; if the culture drugs are relatively viscous, the cells are not easy to flow in the culture drugs, and the cross-stroke method cannot be used. Generally, the spiral method (eccentric oscillation) is used to make the cell fluid inside the culture dish flow in a vortex, which is also to ensure that the cells can be fully mixed with the culture drugs. After that, most of the cells are distributed in the middle area, and the above device has a single oscillation method and cannot meet flexible requirements. Summary of the invention

[0005] The object of the present invention is to provide a cell culture fluid oscillating mechanism for gene detection to solve the problems raised in the above-mentioned background technology.

[0006] The technical solution of the present invention is: a cell culture fluid oscillating mechanism for gene detection, comprising a bottom shell, a top plate slidably arranged on the top of the bottom shell, a through hole is opened in the middle of the top plate of the bottom shell, an annular groove coaxially arranged is opened on the inner side wall of the through hole, and an oscillating table is slidably arranged on the inner side of the annular groove;

[0007] Wherein, a clamping mechanism for clamping a culture dish is provided on the top of the oscillating table;

[0008] Wherein, a cross-pushing mechanism is arranged inside the bottom shell, and the oscillation platform realizes cross-oscillation through the cross-pushing mechanism;

[0009] Wherein, an eccentric shaking mechanism is arranged inside the bottom shell, and the oscillation table performs eccentric shaking through the eccentric shaking mechanism.

[0010] Preferably, vibration isolation pads are fixed at the four corners of the bottom of the bottom shell.

[0011] Preferably, the clamping mechanism comprises a centering structure and a plurality of sliders, a pressure plate is fixed on the top of each slider, and the plurality of sliders perform centering movement through the centering structure.

[0012] Preferably, the centering structure includes a fixed ring, an intermediate ring and a rotating ring, one end of the intermediate ring is fixed on an oscillating table, the other end of the intermediate ring is rotatably installed in the rotating ring, the fixed ring is coaxially arranged with the rotating ring, the fixed ring is located above the rotating ring, and both sides of the fixed ring extend downward until it is fixed on the oscillating table, a plurality of recesses with the central axis as the center and distributed in an equidistant ring are provided in the fixed ring, a plurality of sliders are slidably inserted in the recesses, a plurality of inclined grooves with the central axis as the center and distributed in an equidistant ring are provided on the annular surface of the rotating ring, a cylindrical block is rotatably installed at the bottom of the slider, and a plurality of the cylindrical blocks are respectively arranged in the inclined grooves.

[0013] Preferably, the centering structure also includes an extension plate, a first rotating block, a second rotating block, a double-section telescopic cylinder and a tension spring, one end of the extension plate is fixed on the rotating ring, the other end of the extension plate forms a rotational fit with the first rotating block, one end of the second rotating block is rotatably mounted on the oscillation table, the two ends of the double-section telescopic cylinder are respectively fixed on the first rotating block and the second rotating block, the two ends of the tension spring are respectively fixed on the first rotating block and the second rotating block, and the tension spring is sleeved on the double-section telescopic cylinder.

[0014] The cam is an angular channel that is fixedly provided with a second end of a toothed plate and a toothed plate, and the cam is provided with a pair of teeth to form a rotation axis, and the rotation axis is connected with the toothed plate at two ends thereof.

[0015] Preferably, a servo motor is fixed to the outside of the intermediate cylinder, a second bevel gear is fixed to the outside of the intermediate cylinder, the output shaft of the servo motor extends into the intermediate cylinder and is fixed with a first bevel gear coaxially arranged therewith, and the first bevel gear is meshed with the second bevel gear.

[0016] Preferably, a plurality of electric push rods are fixed at the inner bottom position of the bottom shell, and a lifting plate is commonly fixed to the telescopic ends of the plurality of electric push rods, a first circular hole is opened at the four corners of the bottom of the lifting plate, and a first Z-axis is rotatably installed in the first circular hole, a first synchronous wheel is fixed at the bottom end of each of the first Z-axis, and a first synchronous belt is commonly sleeved on the plurality of first synchronous wheels, a second circular hole is opened at the middle position of the bottom of the lifting plate, and a second Z-axis is rotatably installed in the second circular hole, a second synchronous wheel is fixed at the bottom end of the second Z-axis and the bottom end of one of the first Z-axis, and a second synchronous belt is commonly sleeved on the two second synchronous wheels, a spline sleeve is embedded and fixed at the center of the circular plate, a plurality of spline blocks are fixed on the outer side of the second Z-axis, and the second Z-axis is slidably inserted in the spline sleeve through the spline block, four first jacks adapted to the first Z-axis are opened at the bottom of the oscillation table, and a second jack adapted to the second Z-axis is opened at the middle position of the bottom of the oscillation table.

[0017] The present invention provides a cell culture fluid oscillating mechanism for gene detection by improvement, which has the following improvements and advantages compared with the prior art:

[0018] First, the present invention enables the entire device to perform two different oscillation modes through a cross-pushing mechanism and an eccentric shaking mechanism, which are used to adapt to different culture conditions. If the culture solution is relatively thin, cross oscillation is used, that is, the cross-pushing mechanism is to separate the longitudinal sliding and the transverse sliding of the oscillation table to achieve cross oscillation. If the culture solution is relatively thick, eccentric oscillation is used, that is, the eccentric shaking mechanism makes the entire oscillation table rotate eccentrically, thereby improving the application range of the entire device;

[0019] Second, in the present invention, the experimenter manually turns the rotating ring to rotate it, and the inclined groove on the rotating ring pushes the slider into the recess with the help of the cylindrical block. At this time, the culture dish is placed between the sliders, and then the rotating ring is reset, and the slider contacts the culture dish. At this time, the pressure plate on the slider presses the lid of the culture dish. The clamping mechanism is simple and the operation is convenient and quick. During the oscillation process, the cell fluid cannot flow out. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the internal first-view structure of the present invention;

[0023] Figure 3 It is a schematic diagram of the internal second viewing angle structure of the present invention;

[0024] Figure 4 It is a schematic diagram of the three-dimensional structure of the oscillation table of the present invention;

[0025] Figure 5 It is a schematic diagram of a first three-dimensional structure of the clamping mechanism of the present invention;

[0026] Figure 6 It is a schematic diagram of a second three-dimensional structure of the clamping mechanism of the present invention;

[0027] Figure 7 It is a schematic diagram of the three-dimensional structure of the cross-pushing mechanism of the present invention from a first-view perspective;

[0028] Figure 8 It is a schematic diagram of the three-dimensional structure of the cross-pushing mechanism of the present invention from a second viewing angle;

[0029] Fig. 9 It is a schematic diagram of the three-dimensional structure of the cross-pushing mechanism of the present invention from a third viewing angle;

[0030] Fig.10 This is a schematic diagram of the three-dimensional structure of the circular plate of the present invention from a first viewing angle;

[0031] Fig.11 It is a schematic diagram of the three-dimensional structure of the circular plate of the present invention from a second viewing angle.

[0032] Reference numerals:

[0033] 1. Bottom shell; 2. Vibration isolation pad; 3. Oscillating table; 4. Clamping mechanism; 5. Lifting plate; 6. Electric push rod; 7. First synchronous belt; 8. First synchronous wheel; 9. Second synchronous wheel; 10. Second synchronous belt; 11. Fixed frame; 12. First Z-axis; 13. Second Z-axis; 14. Ring groove; 15. Slide groove; 16. Fixed ring; 17. Sliding block; 18. Pressing plate; 19. Intermediate ring; 20. Rotating ring; 21. First rotating block; 22. Notch; 23 , tension spring; 24, double-section telescopic cylinder; 25, second rotating block; 26, inclined groove; 27, cylindrical block; 28, extension plate; 29, cross push mechanism; 30, intermediate cylinder; 31, first slide bar; 32, first contact block; 33, second contact block; 34, second slide bar; 35, circular plate; 36, convex groove; 37, servo motor; 38, second bevel gear; 39, first bevel gear; 40, rotating cylinder; 41, spline block; 42, slide column; 43, spline sleeve. DETAILED DESCRIPTION

[0034] The present invention is described in detail below, and the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] The present invention provides a cell culture fluid oscillating mechanism for gene detection through improvement. The technical solution of the present invention is:

[0036] like Figures 1 to 11 As shown, an embodiment of the present invention provides a cell culture fluid oscillating mechanism for gene detection, comprising a bottom shell 1, the bottom shell 1 is made of plastic material, a top plate is slidably arranged on the top of the bottom shell 1, a through hole is provided in the middle of the top plate, an inner wall of the through hole is provided with a coaxially arranged annular groove 14, and an oscillating table 3 is slidably arranged on the inner side of the annular groove 14, and a plurality of rolling balls can be embedded in the top and bottom of the annular groove 14, so that the contact mode between the oscillating table 3 and the annular groove 14 is changed to rolling contact, thereby reducing friction damage;

[0037] Among them, a clamping mechanism 4 for clamping a culture dish is arranged on the top of the oscillating table 3, and multiple clamping mechanisms 4 can be arranged on the oscillating table 3 to shake multiple culture dishes at one time;

[0038] A cross-pushing mechanism 29 is disposed inside the bottom shell 1, and the oscillation table 3 realizes cross-oscillation through the cross-pushing mechanism 29;

[0039] The bottom shell 1 is provided with an eccentric shaking mechanism inside, and the oscillation table 3 is eccentrically shaken by the eccentric shaking mechanism.

[0040] Furthermore, combined with Figure 1 Vibration isolation pads 2 are fixed at the four corners of the bottom of the bottom shell 1. The vibration isolation pads 2 are prior art. The setting of the vibration isolation pads 2 isolates the bottom shell 1 from the desktop and prevents the bottom shell 1 from sliding relative to the desktop.

[0041] Combined with Figure 5 and Figure 6 The clamping mechanism 4 described includes a centering structure and a plurality of sliders 17. A pressure plate 18 is fixed to the top of each slider 17. The plurality of sliders 17 perform centering movement through the centering structure.

[0042] Furthermore, combined with Figure 5 The centering structure includes a fixed ring 16, an intermediate ring 19 and a rotating ring 20, one end of the intermediate ring 19 is fixed on the oscillating table 3, and the other end of the intermediate ring 19 is rotatably installed in the rotating ring 20. The fixed ring 16 is coaxially arranged with the rotating ring 20, the fixed ring 16 is located above the rotating ring 20, and both sides of the fixed ring 16 extend downward until they are fixed on the oscillating table 3. A plurality of notches 22 with the central axis as the center and distributed in an equidistant annular shape are provided in the fixed ring 16, and a plurality of sliders 17 are slidably inserted in the notches 22, and a plurality of inclined grooves 26 with the central axis as the center and distributed in an equidistant annular shape are provided on the annular surface of the rotating ring 20, and a cylindrical block 27 is rotatably installed at the bottom of the slider 17, and a plurality of cylindrical blocks 27 are respectively arranged in the inclined grooves 26;

[0043] From the above connection relationship, it can be known that the experimenter manually turns the rotating ring 20 to rotate it, and the inclined groove 26 on the rotating ring 20 pushes the slider 17 into the recess 22 with the help of the cylindrical block 27. At this time, the culture dish is placed between the sliders 17, and then the rotating ring 20 is reset, and the slider 17 contacts the culture dish. At this time, the pressure plate 18 on the slider 17 presses the lid of the culture dish.

[0044] Furthermore, combined with Figure 6The centering structure also includes an extension plate 28, a first rotating block 21, a second rotating block 25, a double-section telescopic cylinder 24 and a tension spring 23. One end of the extension plate 28 is fixed to the rotating ring 20, and the other end of the extension plate 28 forms a rotational fit with the first rotating block 21. One end of the second rotating block 25 is rotatably mounted on the oscillation table 3. Both ends of the double-section telescopic cylinder 24 are respectively fixed to the first rotating block 21 and the second rotating block 25. Both ends of the tension spring 23 are respectively fixed to the first rotating block 21 and the second rotating block 25, and the tension spring 23 is sleeved on the double-section telescopic cylinder 24.

[0045] It can be known from the above connection relationship that when the experimenter manually turns the rotating ring 20 to rotate, the extension plate 28 on the rotating ring 20 stretches the double-section telescopic cylinder 24, the tension spring 23 is stretched, the experimenter releases the rotating ring 20, the tension spring 23 is reset, and the rotating ring 20 is reset.

[0046] Combined with Figure 9-11 The cross-pushing mechanism 29 described includes an intermediate cylinder 30, a rotating cylinder 40, a circular plate 35, a first slide bar 31, a second slide bar 34, a first contact block 32 and a second contact block 33. A plurality of fixing frames 11 are fixed between the outer side of the intermediate cylinder 30 and the inner wall of the bottom shell 1. A rotating hole coaxially arranged therewith is provided at the bottom of the intermediate cylinder 30. One end of the rotating cylinder 40 is rotatably installed in the rotating hole. The other end of the rotating cylinder 40 is coaxially fixed to the circular plate 35. A convex groove 36 is provided on the top and bottom circular surfaces of the circular plate 35. The convex groove 36 consists of two quarter arc grooves of different diameters and two straight grooves. The two quarter arcs are connected by the straight groove. The arc groove is coaxially arranged with the circular plate 35. The two convex grooves 36 are distributed at ninety degrees. The first slide bar 31 and The second slide bar 34 is respectively slidably inserted on the intermediate cylinder 30, and the first slide bar 31 and the second slide bar 34 are skewed and perpendicular, one end of the first slide bar 31 and the second slide bar 34 are rotatably mounted with a slide column 42, the two slide columns 42 are respectively slidably set in two convex grooves 36, the other ends of the first slide bar 31 and the second slide bar 34 are respectively fixed with a first contact block 32 and a second contact block 33, two slide grooves 15 are provided at the bottom of the oscillation table 3, and the two slide grooves 15 are vertically distributed, a servo motor 37 is fixed on the outside of the intermediate cylinder 30, a second bevel gear 38 is fixed on the outside of the intermediate cylinder 30, the output shaft of the servo motor 37 extends into the intermediate cylinder 30 and is fixed with a first bevel gear 39 coaxially arranged therewith, and the first bevel gear 39 is meshed with the second bevel gear 38;

[0047] It can be seen from the above connection relationship that the servo motor 37 rotates the first bevel gear 39 through the output shaft, and the first bevel gear 39 rotates the circular plate 35 through the second bevel gear 38 meshing with it. Fig.11, the circular plate 35 rotates clockwise, at this time, the second slide bar 34 is in the large-diameter arc groove and does not slide, the slide column 42 on the first slide bar 31 is in the straight groove, the first slide bar 31 is pushed out, the first contact block 32 on the first slide bar 31 pushes the oscillation table 3, and the second contact block 33 slides with the oscillation table 3 until entering the large-diameter arc groove and does not slide, and the slide column 42 on the second slide bar 34 enters the straight groove, the second slide bar 34 slides, the second contact block 33 on the second slide bar 34 pushes the oscillation table 3, the first contact block 32 slides with the oscillation table 3, and pushes the oscillation table 3 to realize cross oscillation.

[0048] Further, a plurality of electric push rods 6 are fixed at the bottom position of the bottom shell 1, and a lifting plate 5 is fixed to the telescopic ends of the plurality of electric push rods 6. A first circular hole is opened at the four corners of the bottom of the lifting plate 5, and a first Z-shaped shaft 12 is rotatably installed in the first circular hole. A first synchronous wheel 8 is fixed to the bottom end of each first Z-shaped shaft 12, and a first synchronous belt 7 is commonly sleeved on the plurality of first synchronous wheels 8. A second circular hole is opened at the middle position of the bottom of the lifting plate 5, and a second Z-shaped shaft 13 is rotatably installed in the second circular hole. The second Z-shaped shaft 13 The bottom end of the oscillating table 3 and the bottom end of one of the first Z-shaped shafts 12 are both fixed with a second synchronous wheel 9, and the two second synchronous wheels 9 are jointly sleeved with a second synchronous belt 10. A spline sleeve 43 is embedded and fixed at the center of the circular plate 35, and a plurality of spline blocks 41 are fixed on the outside of the second Z-shaped shaft 13. The second Z-shaped shaft 13 is slidably plugged into the spline sleeve 43 through the spline blocks 41. Four first jacks adapted to the first Z-shaped shaft 12 are provided at the bottom of the oscillating table 3, and a second jack adapted to the second Z-shaped shaft 13 is provided at the middle position of the bottom of the oscillating table 3;

[0049] Supplementary explanation is given to the first Z-axis 12 and the second Z-axis 13. The first Z-axis 12 and the second Z-axis 13 are parallel to each other.

[0050] It can be seen from the above connection relationship that after the clamping mechanism 4 is completely fixed to the culture dish, Figure 1 It is in the initial state. When the culture fluid becomes viscous, the servo motor 37 is started. The servo motor 37 rotates the first bevel gear 39 through the output shaft. The first bevel gear 39 rotates the circular plate 35 through the second bevel gear 38 meshing therewith. The spline sleeve 43 on the circular plate 35 rotates the second Z-axis 13, and the second synchronous wheel 9 on the second Z-axis 13 rotates. The two second synchronous wheels 9 rotate synchronously through the second synchronous belt 10, one of the first Z-axis 12 rotates synchronously with the second Z-axis 13, and the four first synchronous wheels 8 rotate synchronously through the first synchronous belt 7, that is, the four first Z-axis 12 rotate synchronously, thereby the oscillation table 3 rotates eccentrically, the entire culture dish rotates eccentrically, and the cell fluid rotates in a vortex in the culture dish.

[0051] Working principle: The experimenter manually turns the rotating ring 20 to rotate, and the extension plate 28 on the rotating ring 20 stretches the double-section telescopic cylinder 24, the tension spring 23 is stretched, and the inclined groove 26 on the rotating ring 20 pushes the slider 17 into the notch 22 with the help of the cylindrical block 27. At this time, the culture dish is placed between the sliders 17. The experimenter loosens the rotating ring 20, and the tension spring 23 is reset to reset the rotating ring 20. All sliders 17 are in contact with the culture dish. At this time, the pressure plate 18 on the slider 17 presses the lid of the culture dish.

[0052] After the culture dish is completely fixed by the clamping mechanism 4, Figure 1 It is in the initial state. When the culture fluid becomes viscous, the servo motor 37 is started. The servo motor 37 rotates the first bevel gear 39 through the output shaft. The first bevel gear 39 rotates the circular plate 35 through the second bevel gear 38 meshing therewith. The spline sleeve 43 on the circular plate 35 rotates the second Z-shaped shaft 13. The second synchronous wheel 9 on the second Z-shaped shaft 13 rotates. The two second synchronous wheels 9 rotate synchronously through the second synchronous belt 10. One of the first Z-shaped shafts 12 rotates synchronously with the second Z-shaped shaft 13. The four first synchronous wheels 8 rotate synchronously through the first synchronous belt 7, that is, the four first Z-shaped shafts 12 rotate synchronously, thereby the oscillating table 3 rotates eccentrically, the entire culture dish rotates eccentrically, and the cell fluid rotates in a vortex in the culture dish;

[0053] If the added culture medium is relatively thin, the electric push rod 6 is shortened, the first Z-axis 12 and the second Z-axis 13 move downward, and the top of the bottom shell 1 moves downward. At this time, the two slide grooves 15 on the oscillating table 3 are respectively inserted into the first contact block 32 and the second contact block 33, and the electric push rod continues to contract, so that the first Z-axis 12 and the second Z-axis 13 are respectively pulled out of the first jack and the second jack, and the servo motor 37 rotates the first bevel gear 39 through the output shaft, and the first bevel gear 39 rotates the circular plate 35 through the second bevel gear 38 meshing therewith, and the attached Fig.11 , the circular plate 35 rotates clockwise, at this time, the second slide bar 34 is in the large-diameter arc groove and does not slide, the slide column 42 on the first slide bar 31 is in the straight groove, the first slide bar 31 is pushed out, the first contact block 32 on the first slide bar 31 pushes the oscillation table 3, and the second contact block 33 slides with the oscillation table 3 until entering the large-diameter arc groove and does not slide, and the slide column 42 on the second slide bar 34 enters the straight groove, the second slide bar 34 slides, the second contact block 33 on the second slide bar 34 pushes the oscillation table 3, the first contact block 32 slides with the oscillation table 3, and pushes the oscillation table 3 to realize cross oscillation.

[0054] The above description enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell culture fluid oscillating mechanism for gene detection, comprising a bottom shell (1), characterized in that: A top plate is slidably disposed on the top of the bottom shell (1), a through hole is provided in the middle of the top plate, an annular groove (14) coaxially disposed therewith is provided on the inner side wall of the through hole, and an oscillating table (3) is slidably disposed inside the annular groove (14); Wherein, a clamping mechanism (4) for clamping a culture dish is provided on the top of the oscillating table (3); Wherein, a cross-pushing mechanism (29) is arranged inside the bottom shell (1), and the oscillation platform (3) realizes cross-oscillation through the cross-pushing mechanism (29); Wherein, an eccentric shaking mechanism is arranged inside the bottom shell (1), and the oscillation table (3) performs eccentric shaking through the eccentric shaking mechanism.

2. A cell culture fluid oscillating mechanism for gene detection according to claim 1, characterized in that: Vibration isolation pads (2) are fixed at the four corners of the bottom of the bottom shell (1).

3. The cell culture fluid oscillating mechanism for gene detection according to claim 1, characterized in that: The clamping mechanism (4) comprises a centering structure and a plurality of sliders (17), a pressing plate (18) is fixed on the top of each slider (17), and the plurality of sliders (17) perform centering movement through the centering structure.

4. The cell culture fluid oscillating mechanism for gene detection according to claim 3, characterized in that: The centering structure comprises a fixed ring (16), an intermediate ring (19) and a rotating ring (20), one end of the intermediate ring (19) is fixed on the oscillating table (3), and the other end of the intermediate ring (19) is rotatably mounted in the rotating ring (20), the fixed ring (16) and the rotating ring (20) are coaxially arranged, the fixed ring (16) is located above the rotating ring (20), and both sides of the fixed ring (16) extend downward until they are fixed on the oscillating table (3), a plurality of notches (22) with the central axis as the center and distributed in an equidistant annular pattern are provided in the ring of the fixed ring (16), a plurality of sliders (17) are respectively slidably inserted in the notches (22), a plurality of inclined grooves (26) with the central axis as the center and distributed in an equidistant annular pattern are provided on the annular surface of the rotating ring (20), a cylindrical block (27) is rotatably mounted at the bottom of the slider (17), and a plurality of the cylindrical blocks (27) are respectively arranged in the inclined grooves (26).

5. The cell culture fluid oscillating mechanism for gene detection according to claim 4, characterized in that: The centering structure further comprises an extension plate (28), a first rotating block (21), a second rotating block (25), a double-section telescopic cylinder (24) and a tension spring (23); one end of the extension plate (28) is fixed on the rotating ring (20); the other end of the extension plate (28) is rotatably matched with the first rotating block (21); one end of the second rotating block (25) is rotatably mounted on the oscillation table (3); two ends of the double-section telescopic cylinder (24) are respectively fixed on the first rotating block (21) and the second rotating block (25); two ends of the tension spring (23) are respectively fixed on the first rotating block (21) and the second rotating block (25); and the tension spring (23) is sleeved on the double-section telescopic cylinder (24).

6. The cell culture fluid oscillating mechanism for gene detection according to claim 1, characterized in that: The cross-pushing mechanism (29) comprises an intermediate cylinder (30), a rotating cylinder (40), a circular plate (35), a first sliding rod (31), a second sliding rod (34), a first contact block (32) and a second contact block (33); a plurality of fixing frames (11) are fixed between the outer side of the intermediate cylinder (30) and the inner wall of the bottom shell (1); a rotating hole coaxially arranged with the intermediate cylinder (30) is provided at the bottom; one end of the rotating cylinder (40) is rotatably mounted in the rotating hole; the other end of the rotating cylinder (40) is coaxially fixed with the circular plate (35); a convex groove (36) is provided on the top and bottom circular surfaces of the circular plate (35); the convex groove (36) comprises two quarter arc grooves of different diameters and two straight grooves; the two The quarter arcs are connected through a straight groove, the arc groove is coaxially arranged with the circular plate (35), and the two convex grooves (36) are distributed at ninety degrees. The first slide bar (31) and the second slide bar (34) are respectively slidably inserted on the intermediate cylinder (30), and the first slide bar (31) and the second slide bar (34) are skewed and perpendicular. One end of the first slide bar (31) and the second slide bar (34) are both rotatably mounted with a slide column (42), and the two slide columns (42) are respectively slidably arranged in the two convex grooves (36). The other ends of the first slide bar (31) and the second slide bar (34) are respectively fixed with a first contact block (32) and a second contact block (33). The bottom of the oscillation table (3) is provided with two slide grooves (15), and the two slide grooves (15) are distributed vertically.

7. The cell culture fluid oscillating mechanism for gene detection according to claim 6, characterized in that: A servo motor (37) is fixed on the outside of the intermediate cylinder (30), a second bevel gear (38) is fixed on the outside of the intermediate cylinder (30), an output shaft of the servo motor (37) extends into the intermediate cylinder (30) and is fixed with a first bevel gear (39) coaxially arranged therewith, and the first bevel gear (39) is meshed with the second bevel gear (38).

8. The cell culture fluid oscillating mechanism for gene detection according to claim 6, characterized in that: A plurality of electric push rods (6) are fixed at the bottom of the bottom shell (1), and a lifting plate (5) is fixed to the telescopic ends of the plurality of electric push rods (6). A first circular hole is provided at the four corners of the bottom of the lifting plate (5), and a first Z-shaped shaft (12) is rotatably mounted in the first circular hole. A first synchronous wheel (8) is fixed at the bottom end of each of the first Z-shaped shafts (12), and a first synchronous belt (7) is commonly sleeved on the plurality of first synchronous wheels (8). A second circular hole is provided at the middle of the bottom of the lifting plate (5), and a second Z-shaped shaft (13) is rotatably mounted in the second circular hole. The bottom end of the second Z-shaped shaft (13) is provided with a first synchronous wheel (8). A second synchronous wheel (9) is fixed to the bottom end of each of the first Z-shaped shafts (12), and a second synchronous belt (10) is sleeved on the two second synchronous wheels (9). A spline sleeve (43) is embedded and fixed at the center of the circular plate (35), and a plurality of spline blocks (41) are fixed to the outer side of the second Z-shaped shaft (13). The second Z-shaped shaft (13) is slidably inserted into the spline sleeve (43) through the spline blocks (41). Four first plug holes matching the first Z-shaped shaft (12) are provided at the bottom of the oscillating table (3), and a second plug hole matching the second Z-shaped shaft (13) is provided at the middle position of the bottom of the oscillating table (3).

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