Saccharomyces cerevisiae fermentation product as well as preparation method and application thereof

Abstract

The invention relates to cosmetics and raw materials thereof, in particular to a preparation method of a saccharomyces cerevisiae fermentation product, which comprises the following steps: (i) fermentation: fermenting saccharomyces cerevisiae with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.19732, filtering or centrifuging to remove thalli, and taking fermentation filtrate or supernate; (ii) post-treatment: adding activated carbon into the fermentation filtrate or supernate, and stirring for 0.5-24 hours at the temperature of 4-40 DEG C and the speed of 0-500 rpm; and removing the activated carbon, and taking filtrate. The Saccharomyces cerevisiae fermentation product obtained by the method still has a good sunscreen effect, the influence of the Saccharomyces cerevisiae fermentation product on the viscosity of a cosmetic formula is reduced, the smell is improved, the Saccharomyces cerevisiae fermentation product can be better applied to sunscreen products, and the product stability and the use experience are improved. The method is convenient to operate, can be used for large-scale rapid treatment, and has a good application prospect.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to a brewer's yeast fermentation product with sunscreen function, and a preparation method and application thereof. Background Art

[0002] Yeast has a wide range of uses. Traditionally, it is used in fermented foods. Its extracts have moisturizing and revitalizing effects, and its cell wall polysaccharides have antioxidant properties. Wild yeast from the polar-like environment of the Himalayas has adapted to low temperatures, hypoxia, strong radiation, and other environments. Yeast has broad application prospects in many fields such as clean energy, food industry, bio-enzyme industry, and synthetic biology.

[0003] Sunlight is the source of ultraviolet radiation in daily life. The proportions of UVB and UVA in the solar radiation reaching the earth's surface are 5% and 95% respectively. Long-term and large amounts of ultraviolet radiation can cause serious damage to the human body. It can not only increase the skin temperature through its thermal radiation effect, dilate and congest capillaries, increase epidermal water evaporation, etc., directly causing adverse effects on the skin and accelerating skin aging, but also lead to skin pigmentation, the formation of freckles, chloasma and other spots, as well as the occurrence of various solar skin diseases.

[0004] CN114081862A discloses a kind of highland barley wine yeast, with the deposit number of CGMCC No.19732. The fermentation product obtained by the brewer's yeast has the effects of removing free radicals, inhibiting tyrosinase, preventing oxidative damage, preventing ultraviolet UVA / UVB from damaging the skin, and promoting collagen secretion, and promoting the proliferation and vitality of dermal fibroblasts and epidermal cells, and can be used as an active ingredient for preparing cosmetics. After removing the bacteria and impurities from the fermentation liquid, 0.1-5.0% powdered or granular activated carbon is added to the filtrate, and the filtrate is left to stand or stirred at 4-30°C for 0.5-20 hours, and the filtrate with a final solid content of about 1-2wt% is adjusted as the fermentation product for the experiment; its pH is 6.11 and the conductivity is 2070μS / cm.

[0005] In subsequent product development, it was found that this type of fermentation product had poor viscosity and was difficult to compound with other cosmetic ingredients, which greatly reduced the viscosity of the resulting formula product and made it impossible to form a sunscreen product that could be used for application, such as sunscreen lotion, sunscreen cream, etc.

[0006] Sunscreen cosmetics, such as sunscreen lotion, sunscreen cream, sunscreen spray, sunscreen stick, etc., are difficult to form into dosage forms if their raw materials have low viscosity, or the product stability is poor, or it is too thin, which makes it difficult to apply, affecting the user experience. Yeast fermentation filtrate often has low viscosity, and even after concentration or drying, it still has such problems when used in products. Therefore, although the yeast fermentation product has the effects of sun protection, whitening, and preventing photoaging, its application in sunscreen products is still limited. Moreover, even if the yeast fermentation filtrate is treated with conventional activated carbon, it cannot completely remove bad odors such as sour smell and sweat odor.

[0007] Therefore, it is necessary to improve the existing technology to increase the viscosity of yeast fermentation filtrate, improve the stability of sunscreen products, and improve their bad smell so that they can be used in cosmetics, especially cosmetics with sunscreen function. Summary of the invention

[0008] The invention aims to provide a brewer's yeast fermentation product with increased viscosity, removed bad smell and enhanced fragrance, and use the fermentation product in cosmetics, especially sunscreen products.

[0009] The scheme of the present invention is:

[0010] A brewer's yeast fermentation product, the preparation method of which comprises the following steps:

[0011] (i) Fermentation: After fermentation of Saccharomyces cerevisiae with the accession number CGMCC No. 19732, the cells are removed by filtration or centrifugation to obtain the fermentation filtrate or supernatant;

[0012] (ii) Post-treatment: adding activated carbon to the fermentation filtrate or supernatant, stirring at 4-40° C. and 0-500 rpm for 0.5-24 h; removing the activated carbon.

[0013] The yeast fermentation product is the filtrate obtained after the treatment in step (ii), or a diluted solution thereof, or a concentrated solution or dry powder obtained after further concentration or drying.

[0014] The fermentation filtrate or supernatant obtained in step (i) has a solid content of 0.5-3wt% and a pH value of 6-7.

[0015] The activated carbon described in step (ii) is at least one of wood activated carbon, coconut shell activated carbon or coal activated carbon.

[0016] The amount of activated carbon used is 1-3 wt % of the fermentation filtrate or supernatant, preferably 1-2 wt %.

[0017] The activated carbon can be in the form of granules, powders, honeycombs, or columns, preferably granules or powders, with a particle size of 10-500 meshes.

[0018] A preferred embodiment of the present invention is powdered activated carbon with a particle size of 200-300 meshes; another preferred embodiment is granular activated carbon with a particle size of 10-30 meshes.

[0019] Preferably, the activated carbon is a mixture of wood activated carbon and coal activated carbon, or a mixture of coconut shell activated carbon and coal activated carbon.

[0020] Among them, the mass ratio of wood activated carbon or coconut shell activated carbon to coal activated carbon is 1-10:1, preferably 5-10:1.

[0021] The wood activated carbon is made from tree materials (such as sawdust, firewood or wood blocks), such as pine, fir, willow and the like.

[0022] Preferably, the wood activated carbon is one of pine activated carbon, fir activated carbon, or any mixture thereof.

[0023] More preferably, the activated carbon is obtained by chemical phosphoric acid method, and the pH value is 2-5; preferably, the pH value is 2-4.

[0024] Preferably, in step (ii), the stirring treatment time is 1-6 hours.

[0025] The solid content of the filtrate obtained in step (ii) is 0.2-2.5%, and the pH value is 4-6.

[0026] The method for removing the activated carbon in step (ii) is membrane filtration or centrifugal filtration.

[0027] The membrane filtration equipment is a positive pressure filter, a bag filter or a plate and frame filter; the centrifugal filtration equipment is a plate centrifuge. Preferably, the filter membrane is made of nylon, polypropylene or polyethersulfone, the pore size of the filter membrane is 0.1 to 1 μm, and the filtration pressure is 2 to 6 bar.

[0028] The filtration may be performed with or without a filter aid; the filter aid is perlite or diatomaceous earth; the particle size of the filter aid is 200-300 meshes, and the amount added is 1-3% of the mass of the liquid to be treated.

[0029] The yeast fermentation product, whether used in the form of filtrate, diluent, concentrate, dry powder or reconstituted dry powder for sunscreen products, has a significantly lower effect on the viscosity of the product than the fermentation product before treatment.

[0030] Preferably, the fermentation method of step (i) is:

[0031] (1) First fermentation stage: the seed bacterial liquid is added to the fermentation medium and fermented at 15-30°C for 12-24 hours;

[0032] Preferably, the fermentation medium is YPD medium or modified YPD medium; the fermentation conditions are: stirring at 200-400 rpm, ventilation 0-4.0 vvm;

[0033] (2) Second fermentation stage: Supplementing sugars and proteins into the fermentation medium to make the sugar concentration in the fermentation medium 0.1wt%-5.0wt% and the protein concentration 0.1wt%-2.0wt%, and continuing fermentation at 15-30°C for 12-72 hours;

[0034] Preferably, the concentration of carbohydrates is 1wt%-2wt%; the concentration of protein is 0.1wt%-2.0wt%, preferably 0.5wt%-1.5wt%; the preferred fermentation time is 48-72 hours; the fermentation conditions are: stirring at 0-400rpm, 0.2-2.0vvm ventilation;

[0035] In step (2), the protein substance is at least one of yeast extract (yeast extract or yeast extract powder), peptone or yeast peptone; the sugar substance is at least one of corn syrup, molasses or glucose and xylose. In the second fermentation stage, the supplementary substance is fed.

[0036] The fermentation product of Saccharomyces cerevisiae obtained by the above method still has good sunscreen effect and can be used to prepare sunscreen cosmetics, including but not limited to: sunscreen lotion, sunscreen cream, sunscreen spray, sunscreen stick and other products claiming sunscreen. The fermentation product, calculated on a dry weight basis, accounts for 0.0001-10% of the total weight of the sunscreen cosmetics, preferably 0.001-5% of the total weight of the cosmetics. The total weight of the fermentation filtrate after treatment in the cosmetics is 0.5%-10%.

[0037] The beneficial effect of the present invention is that the performance of the fermentation product of brewer's yeast of GMCC No. 19732 is improved, while maintaining its efficacy, its influence on the viscosity of the cosmetic formula is reduced, and the odor is improved, so that it can be better applied to sunscreen products, and the product stability and use experience are improved. The method is easy to operate, can be processed quickly on a large scale, can process kilograms to tons of fermentation products, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 This is the histochemical image of the UV-irradiated 3D full-thickness skin model stained with hematoxylin and eosin (HE) (10 times)

[0039] Figure 2 Relative thickness of the epidermis in the UV-irradiated 3D full-thickness skin model

[0040] Figure 3Photos of CPDs immunohistochemistry and immunofluorescence staining of UV-irradiated 3D full-thickness skin model

[0041] Figure 4 Relative number of CPDs in UV-irradiated 3D full-thickness skin model

[0042] Figure 5 Photo of LC3B immunofluorescence staining of UV-irradiated 3D full-thickness skin model

[0043] Figure 6 is the relative unit fluorescence intensity of LC3B in the UV-irradiated 3D full-thickness skin model DETAILED DESCRIPTION

[0044] The present invention is described below by specific examples, but the protection scope of the present invention is not limited thereby. Experimental methods without specifying specific operating methods and conditions are selected according to conventional operating methods and experimental conditions, or product instructions.

[0045] Example 1 Preparation of fermentation products

[0046] Saccharomyces cerevisiae is numbered JBA-MBY-JT017 and was patented by the China General Microbiological Culture Collection Center (CGMCC) on April 26, 2020, with the deposit number: CGMCC No.19732.

[0047] The above-mentioned Saccharomyces cerevisiae was plated and a single colony was inoculated into liquid PDB or YPD liquid medium. The culture was shaken at 20-30°C and 0-400 rpm for 16-20 hours until OD 600 The fermentation temperature is preferably 28°C for 16-20 hours.

[0048] Inoculate the seed liquid bacteria into the fermentation medium at a rate of 1%-3% (v / v), and ferment according to the following method:

[0049] (1) First fermentation stage: After the seed bacterial liquid is inoculated into the fermentation medium (YPD medium or modified YPD medium), the medium is stirred at a speed of 200-400 rpm, the ventilation volume is 0-4.0 vvm, and the fermentation is carried out at 15-30° C. for 12-24 hours.

[0050] (2) Second fermentation stage: Supplementing sugars and proteins into the fermentation medium, so that the sugar concentration in the fermentation medium is 0.1wt%-5.0wt%, preferably 1wt%-2wt%; the protein concentration is 0.1wt%-2.0wt%, preferably 0.5wt%-1.5wt%; continuing fermentation at 15-30°C for 12-72 hours, preferably 48-72 hours; stirring speed of 0-400rpm, ventilation volume of 0.2-2.0vvm;

[0051] In step (2), the protein substance is at least one of yeast extract (yeast extract or yeast extract powder), peptone or yeast peptone; and the carbohydrate substance is at least one of corn syrup, molasses or glucose and xylose.

[0052] In the second fermentation stage, the method of supplementing substances is feeding.

[0053] After fermentation, the bacteria are removed by filtration or centrifugation, and the fermentation filtrate or supernatant is taken, the solid content of which is 2-3wt%.

[0054] The fermentation broth is filtered through a column filter, disc centrifuge, tubular centrifuge or membrane filtration to remove bacteria. Membrane technology filtration and sterilization includes plate and frame membrane filtration, ceramic membrane filtration, hollow fiber membrane, etc., and the fermentation sample is filtered with a positive pressure filter, plate and frame filter or ceramic membrane, hollow fiber membrane equivalent to 0.1μm-0.8μm membrane filtration, suitable for plate and frame filter membranes such as polyethersulfone resin (PES), polypropylene (PP), nylon membrane, and column membrane columns such as hollow fiber membrane columns, ceramic membrane columns, etc.

[0055] The collected fermentation filtrate was post-treated by adding 1.6wt% of a mixture of wood (pine) powdered activated carbon with a particle size of 200-300 mesh and coal powdered activated carbon, the mass ratio of wood powdered activated carbon to coal powdered activated carbon was 7:1, and the temperature was controlled at 20-30°C and 50-200rpm for 2h. The above activated carbon was prepared by phosphoric acid activation method, and the pH was 3-4.

[0056] Use membrane filtration or centrifugal filtration to remove activated carbon. The membrane filtration equipment is a positive pressure filter, bag filter or plate and frame filter. The centrifugal filtration equipment is a plate centrifuge with a filtration area of ​​0.03 to 0.3 m 2 The filter membrane is made of nylon, polypropylene or polyethersulfone, the pore size is 0.1-1 μm, and the filtration pressure is 2-6 bar. Filter aids may be used or not. The filter aid is perlite or diatomaceous earth. The particle size of the filter aid is 200-300 mesh. The amount of filter aid added is 1-3% of the mass of the liquid to be treated.

[0057] The sterilized fermentation filtrate treated by the method can be in the scale of kilograms to tons; the filtrate decolorized and deodorized after being treated with activated carbon has a solid content of 1.5-2.5wt%.

[0058] The obtained filtrate can be further concentrated or dried to obtain a concentrated solution or dry powder. The fermentation filtrate, supernatant, concentrated solution or dry powder is the fermentation product of Saccharomyces cerevisiae.

[0059] Through the above-mentioned activated carbon treatment, unpleasant odors such as sour smell and sweat smell in the fermentation filtrate can also be removed, thereby improving the user experience.

[0060] Comparative Example

[0061] The same method as in Example 1 was used to obtain a fermentation broth, to which 1.6 wt% of phosphoric acid-activated wood granular activated carbon with a particle size of 10-30 meshes was added; or 1.6 wt% of phosphoric acid-activated coal-based activated carbon with a particle size of 200-300 meshes; or 2 wt% of a mixture of non-phosphoric acid-activated wood powder activated carbon and coal-based powder activated carbon (the mass ratio was 7:1), with an activated carbon particle size of 10-30 meshes. The same conditions as in Example 1 were used for stirring treatment and removal of activated carbon. The temperature was controlled at 20-30° C. and stirred at 50-200 rpm for 2 h.

[0062] The effects of the filtrates obtained by the treatment processes of Example 1 and the comparative example on the viscosity of the formulation system and the component analysis are shown in Table 1.

[0063] Table 1: Effect of filtrate from different treatment processes on the viscosity of the formulation system

[0064]

[0065] Table 2: GC-MS component analysis before and after post-treatment

[0066]

[0067]

[0068] Taking sunscreen lotion as an example, prepare a sunscreen cosmetic containing the following components and mass percentages:

[0069] The balance is deionized water, steareth-21 2.0, bis-ethylhexyloxyphenol methoxyphenyl triazine 3.0, ethylhexyl methoxycinnamate 9.0, ethylhexyl triazone 2.5, acrylates / C10-30 alkyl acrylate crosspolymer 0.2, C14-22 alcohol / C12-20 alkyl glucoside 2.0, caprylic / capric triglyceride 4.0, C12-15 alcohol benzoate 4.0, disodium EDTA 0.05, styrene / acrylates copolymer 2.0, triacontyl PVP 1.0 and the fermentation product (filtrate) of Example 1.

[0070] The effect on the viscosity of the formula was determined by the following method: After 5% of the yeast fermentation liquid was added to the sunscreen formula in Example 2, the change in the viscosity of the sunscreen liquid before and after the addition of the fermentation liquid was measured by a Brookfield viscometer at room temperature, and the change value was calculated. It was found that the treated fermentation liquid was used in the sunscreen system, and the effect on the sunscreen system was small, as shown in Table 1. The product stability test was determined by the following method: the sunscreen formula was placed in light, low temperature (-20°C), high temperature (45°C), and room temperature conditions to observe the changes in the formula color, odor, and properties, and the stability was indicated by "+". At the same time, it can be seen from Table 2 that the short-chain fatty acids such as acetic acid, butyric acid, 3-methylbutyric acid, and 2-methylpropionic acid in the fermentation product after treatment were significantly reduced. Such substances usually show sour odors, sweat odors, etc., and the special odors are removed by post-treatment processes, the odor of the fermentation filtrate is improved, and it is more convenient to use in cosmetic formulas. In summary, the compatibility and compatibility of the sunscreen formula with the addition of fermentation filtrate are well improved.

[0071] The activated carbon in Example 1 was replaced with granular activated carbon with a particle size of 10-30 mesh, and the other conditions remained unchanged. The obtained filtrate was used in the above formulation system, and the viscosity was reduced by about 14%.

[0072] When the wood activated carbon in Example 1 is fir activated carbon, or coconut shell activated carbon is used instead of wood activated carbon, the effect remains unchanged.

[0073] Example 2 Application in sunscreen cosmetics

[0074] The fermentation filtrate treated with activated carbon obtained in Example 1 is added to sunscreen cosmetics, including but not limited to: sunscreen lotions, sunscreen creams, sunscreen sprays, sunscreen sticks and other products claiming to be sunscreen. The fermentation filtrate in the sunscreen cosmetics accounts for 0.0001-10% of the total weight, preferably 0.1-5% of the total weight of the cosmetics.

[0075] Taking sunscreen lotion as an example, prepare a sunscreen cosmetic containing the following mass percentage components:

[0076] The balance is deionized water, steareth-21 2.0, bis-ethylhexyloxyphenol methoxyphenyl triazine 3.0, ethylhexyl methoxycinnamate 9.0, ethylhexyl triazone 2.5, acrylates / C10-30 alkyl acrylate crosspolymer 0.2, C14-22 alcohol / C12-20 alkyl glucoside 2.0, caprylic / capric triglyceride 4.0, C12-15 alcohol benzoate 4.0, disodium EDTA 0.05, styrene / acrylates copolymer 2.0, triacontyl PVP 1.0 and the fermentation product (filtrate) of Example 1.

[0077] The content of the fermentation filtrate is preferably 0.1-10%, more preferably 1-5%. The sunscreen lotion samples prepared with fermentation filtrate of different concentrations were taken with a pipette, (0.0276±0.0003) g of the sample was evenly applied on a PMMA plate (5 cm×5 cm), the sample was evenly applied with an index finger wearing a disposable finger cot within a specified time, and the sample was dried in the dark for 30 minutes under constant temperature and humidity conditions, and the SPF and PA values ​​of the sample were tested with UV-2000s, and 10 different points were tested on each plate.

[0078] The added amounts are all weight percentages (w / w). After being placed under light conditions and high temperature conditions of 45°C for 3 months, the sunscreen cosmetics prepared from the fermentation product filtrate of Example 1 have good product stability, not only improving the SPF and PA values, but also having a good skin feel, being easy to apply, non-sticky, non-greasy, and relatively mild.

[0079] Table 3: List of sun protection factors of sunscreen lotions with added fermentation filtrate

[0080]

[0081] As shown in Table 3, the SPF and PA values ​​of the sunscreen lotion added with the fermentation filtrate are significantly improved, indicating that the fermentation filtrate has the function of a sunscreen agent and can play a certain sunscreen effect.

[0082] Example 3 UV irradiation 3D full-thickness skin model

[0083] The 3D full-thickness skin model constructed using keratinocytes and fibroblasts has similar functions to normal human skin and is an important tool for evaluating the skin's ability to protect against ultraviolet radiation. The 3D full-thickness skin model reconstructed in vitro was used to evaluate the sunscreen function of the fermentation filtrate. The culture cycle was 46 days in total, with 28 days of dermal culture and 5 days of immersion culture. 5% fermentation filtrate was added from the 21st day, and UVA5J & UVB0.05J irradiation was performed from the 43rd day for 3 consecutive days. A control group and a model group were established, with 3 samples in each group. The full-thickness skin model was analyzed by immunohistochemistry and immunofluorescence staining.

[0084] Ultraviolet B (UVB) radiation can cause DNA damage in skin cells, mainly producing cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4PPs, 6-4 pyrimidine-pyrimidone or 6-4 pyrimidine-pyrimidone). Both photoproducts can cause mutations in DNA characterized by C→T or CC→TT base substitutions. Of the two photoproducts, CPDs account for about 80%, and their repair efficiency is low. They can also induce the skin to produce a variety of inflammatory factors such as tumor necrosis factor α (TNF-α), and are considered to be important mediators of UVB damage to the skin. Under normal circumstances, these two photoproducts are mainly cleared and repaired by the nucleotide excision repair (NER) pathway. Xeroderma pigmentosum gene C (XPC) is the earliest damage recognition protein in the NER pathway, and plays a signaling role in the DNA damage response. Studies have shown that the loss of its expression often leads to the inability to repair damaged DNA normally. When DNA damage occurs in cells, the DNA repair system is activated. These processes involve the activation of a series of proteins (such as AMPK, ULK1, etc.). These proteins are closely related to the activation of cellular autophagy while participating in key pathways of DNA repair. AMPK is a highly conserved serine / threonine protein kinase that regulates energy metabolism. Activated AMPK activates ULK1 by directly phosphorylating its Ser317 site, thereby initiating autophagy. UVB rapidly induces autophagy by activating AMPK, which can upregulate XPC protein and promote the NER pathway. NER further regulates DNA damage repair by clearing and repairing CPD. In addition, studies have shown that knocking down AMPK can reduce CPD repair after UVB irradiation, so autophagy can be linked to the positive regulation of UVB-induced DNA damage repair.

[0085] Autophagy is a highly conserved lysosomal degradation pathway in eukaryotic cells, which can degrade intracellular components into small molecules and release energy to maintain the dynamic cycle of intracellular substances and energy. The earliest widely recognized role of autophagy is to help cells resist harsh environments. For example, when the environment is nutrient-deficient, cells upregulate autophagy levels to degrade some unnecessary macromolecules in the cell into amino acids, glucose, fatty acids, etc. for cell use. In addition, if abnormal conditions occur in the cell, such as endoplasmic reticulum stress (ER-stress), mitochondrial damage, and aggregate accumulation, the autophagosome will encapsulate these damaged organelles or aggregates, isolating them from the cytoplasm to avoid damage to the cell, and promoting their degradation to maintain cell homeostasis. LC3 (microtubule associated protein 1light chain 3, LC3) is a key molecule for the fusion of autophagosomes and lysosomes. During the autophagy process, LC3 is cleaved by the protease ATG4 to produce LC3-Ⅰ, which combines with phosphatidylethanolamine to form LC3-Ⅱ and is anchored on the autophagosome membrane. The function of LC3-Ⅱ is to act as an adaptor protein, interacting with the p62 protein to allow the autophagosome to engulf the substrate. LC3B, as a type of LC3 protein, can also be used as a marker for autophagy.

[0086] The tissue structure of the cultured 3D full-thickness skin model was analyzed by hematoxylin-eosin (HE) staining. UBA&UVB irradiation will cause the epidermis to become thinner. Immunofluorescence staining was used to analyze the expression of CPDs and LC3B in the 3D full-thickness skin model. The immunofluorescence analysis method was to take pictures of the control group, model group and sample group at the same exposure and background, and then use Image-Pro Premier 9.2 to perform optical density analysis on the pictures. The statistical method t-test was used for analysis. p < 0.05 indicated statistical differences, marked with "*". The results are shown in the figure. Figure 1-6 .

[0087] Depend on Figure 1 and 2 It can be seen that after UV irradiation, the stratum corneum of the skin model becomes thinner, and after adding 5% fermentation filtrate, the stratum corneum becomes significantly thicker, indicating that the fermentation filtrate can prevent UV from damaging the skin, that is, it has a sunscreen effect.

[0088] Figure 3 and Figure 4 The results showed that after UV irradiation, CPDs were produced in the stratum corneum of the skin model, and the addition of 5% fermentation filtrate significantly reduced the number of CPDs, indicating that the fermentation filtrate can reduce the photodamage caused by UV irradiation, that is, it plays a certain sunscreen effect.

[0089] Figure 5 and Figure 6The results showed that after UV irradiation, the LC3B content in the skin model was significantly reduced, and the addition of 5% fermentation filtrate significantly increased the LC3B content, indicating that the fermentation filtrate increased the level of cellular autophagy after UV irradiation, resisted UV radiation, and exerted a sun protection effect.

[0090] The above are preferred embodiments of the present invention, which are used to illustrate the technical concept and features of the present invention. The purpose is to enable people familiar with the technology of this project to understand the content of the present invention and implement it accordingly, and it cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a brewer's yeast fermentation product, characterized in that: The following steps are involved: (i) Fermentation: After fermentation of Saccharomyces cerevisiae with the accession number CGMCC No. 19732, the cells are removed by filtration or centrifugation to obtain the fermentation filtrate or supernatant; (ii) Post-treatment: adding activated carbon to the fermentation filtrate or supernatant, stirring at 4-40° C. and 0-500 rpm for 0.5-24 h; removing the activated carbon and taking the filtrate.

2. The preparation method according to claim 1, characterized in that: The activated carbon described in step (ii) is at least one of wood activated carbon, coconut shell activated carbon or coal-based activated carbon, and its usage is 1-3wt% of the fermentation filtrate or supernatant.

3. The preparation method according to claim 1, characterized in that: The activated carbon in step (ii) is in the form of granules, powders, honeycombs or columns.

4. The preparation method according to claim 1, characterized in that: The activated carbon in step (ii) is in granular or powder form, and its particle size is 10-500 meshes.

5. The preparation method according to claim 1, characterized in that: The activated carbon described in step (ii) is in powder form with a particle size of 200-300 meshes.

6. The preparation method according to claim 2, characterized in that: The activated carbon is a mixture of wood activated carbon and coal activated carbon, or a mixture of coconut shell activated carbon and coal activated carbon.

7. The preparation method according to claim 6, characterized in that: The mass ratio of the wood activated carbon or coconut shell activated carbon to the coal activated carbon is 1-10:

1.

8. The preparation method according to claim 6, characterized in that: The mass ratio of the wood activated carbon or coconut shell activated carbon to the coal activated carbon is 5-10:

1.

9. The preparation method according to claim 6, characterized in that: The wood activated carbon is pine activated carbon, fir activated carbon or a mixture thereof.

10. The preparation method according to any one of claims 1 to 9, characterized in that: The activated carbon is obtained by chemical phosphoric acid method, and the pH value is 2-5.

11. The preparation method according to claim 1, characterized in that: The filtrate obtained in step (ii) is diluted to obtain a diluted solution, or is further concentrated or dried to obtain a concentrated solution or dry powder.

12. A brewer's yeast fermentation product, characterized in that: The method is obtained by the preparation method according to any one of claims 1 to 11.

13. Use of the brewer's yeast fermentation product according to claim 12 in the preparation of sunscreen cosmetics.

14. A sunscreen cosmetic, characterized in that: Contains the brewer's yeast fermentation product according to claim 12.

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