Whitening composition containing achillea millefolium and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a whitening composition containing achillea millefolium and application of the whitening composition. The composition comprises a achillea millefolium extract and a barley malt extract. The composition provided by the invention is stable in performance and can be stored for a long time; the whitening mask has excellent whitening, spot-fading and moisturizing effects.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, in particular to a whitening composition containing yarrow and application thereof. Background Art

[0002] Yarrow (Achillea millefolium), also known as "melaleuca" or "yarrow", is a common medicinal plant belonging to the Asteraceae family. Its name comes from the legendary Greek hero Achilles, who is said to have used the plant to heal wounds from battle. Yarrow has been traditionally used to stop bleeding, to help heal wounds and bleeding; it has anti-inflammatory properties, helping to relieve symptoms associated with inflammation; and its essential oil is used in aromatherapy for its relaxing and calming effects.

[0003] Achillea millefolium is a plant with a variety of active ingredients that give it benefits in traditional herbal medicine and modern skin care products. The main components of yarrow include: essential oils, flavonoids, monoterpenes, bitter principles, tannins, polyphenols, amino acids, vitamins, terpenes and plant sterols.

[0004] Barley malt extract is a natural food derived from grains. It is rich in maltose, fructose, glucose, protein, small molecule peptides, essential amino acids for the human body, and multiple vitamins and minerals. In addition, barley malt extract also contains β-glucan and tocopherol with health functions. Its chemical components include α- and β-amylase, catalytic enzyme, malt alkaloids, adenine, choline, vitamins B, D, E, cytochrome C, etc.

[0005] The invention provides a whitening composition containing yarrow and application thereof. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a whitening composition containing yarrow, wherein the composition comprises: yarrow extract and barley malt extract.

[0007] As an embodiment of the present invention, the mass ratio of the yarrow extract to the barley malt extract is 10:(0.01-1).

[0008] As an embodiment of the present invention, the mass ratio of the yarrow extract to the barley malt extract is 10:0.02.

[0009] As an embodiment of the present invention, the preparation method of the barley malt extract is to obtain the barley malt extract by fermenting and extracting barley malt.

[0010] As an embodiment of the present invention, the whitening composition containing yarrow further comprises roselle fermentation liquid.

[0011] As an embodiment of the present invention, the whitening composition containing yarrow further comprises a Japanese callicarpa extract.

[0012] The second aspect of the present invention provides a whitening and spot-lightening skin care composition, the preparation raw materials of which include the whitening composition containing yarrow.

[0013] As an embodiment of the present invention, the composition is in the form of an aqueous solution, an emulsion, a cream, an ointment, or a gel.

[0014] As an embodiment of the present invention, the whitening and spot-lightening skin care composition further comprises 1-methylhydantoin-2-imide and diglucosyl gallic acid.

[0015] As an embodiment of the present invention, the mass ratio of the 1-methylhydantoin-2-imide to diglucosyl gallic acid is (7-10):1.

[0016] By adopting the above technical solution, the present invention has the following beneficial effects:

[0017] The composition provided by the invention has stable performance and can be stored for a long time; it has excellent whitening, spot-lightening and moisturizing effects. DETAILED DESCRIPTION

[0018] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] The present invention provides a whitening composition containing yarrow, the composition comprising: yarrow extract and barley malt extract; as an embodiment of the present invention, the mass ratio of the yarrow extract to the barley malt extract is 10:(0.01-1); as an embodiment of the present invention, the mass ratio of the yarrow extract to the barley malt extract is 10:0.02.

[0020] Yarrow Extract

[0021] The preparation method of the Yarrow extract of the present invention is as follows:

[0022] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0023] Barley malt extract

[0024] In the present invention, the preparation method of the barley malt extract is as follows:

[0025] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0026] (2) After soaking, draining the water, germinating the barley in a germination device for 4-6 days, taking it out, chopping it, and fermenting it; after fermentation, filtering it, taking the filtrate and evaporating it at a constant temperature to remove part of the solvent, and thus obtaining the barley malt extract.

[0027] The components of the fermentation broth are:

[0028] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0029] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0030] The inoculation amount is: 6 vol%.

[0031] Fermentation conditions were: 30°C, 24h.

[0032] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0033] The whitening composition containing yarrow also contains roselle fermentation liquid. In the present invention, the preparation method of the roselle fermentation liquid is to ferment roselle and barley malt together.

[0034] The yarrow-containing whitening composition further comprises a Japanese callicarpa extract.

[0035] The second aspect of the present invention provides a whitening and spot-lightening skin care composition, the preparation raw materials of which include the whitening composition containing yarrow.

[0036] The whitening and spot-lightening skin care composition further comprises 1-methylhydantoin-2-imide and diglucosyl gallic acid. The mass ratio of the 1-methylhydantoin-2-imide to the diglucosyl gallic acid is (7-10):1; more preferably, the mass ratio of the 1-methylhydantoin-2-imide to the diglucosyl gallic acid is 8:1.

[0037] As one embodiment of the present invention, the present invention provides a whitening and spot-lightening skin care lotion, the lotion comprising: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water added to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0038] Although yarrow has these beneficial skin care properties, its effect as a whitening ingredient may be relatively mild and not as significant as some ingredients specifically for whitening (such as vitamin C, arbutin, etc.). At the same time, the inventors found that yarrow has poor stability, especially when combined with other whitening ingredients, it is easy to precipitate. The inventors found that adding roselle fermentation liquid to the formula and adding EDTA-2Na when preparing yarrow extract can obtain a composition with stable performance and good effect.

[0039] The present invention is further explained below in conjunction with specific implementation modes.

[0040] Example 1

[0041] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0042] The mass ratio is: 10:0.02:2::0.5:2.5:0.31.

[0043] The preparation method of the yarrow extract is as follows:

[0044] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0045] The preparation method of the barley malt extract and roselle fermentation filtrate is as follows:

[0046] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0047] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0048] The components of the fermentation broth are:

[0049] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0050] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0051] The inoculation amount is: 6 vol%.

[0052] Fermentation conditions were: 30°C, 24h.

[0053] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0054] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0055] Example 2

[0056] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0057] The mass ratio is: 10:0.02:2::0.5:2.5:0.31.

[0058] The preparation method of the yarrow extract is as follows:

[0059] Freshly picked yarrow (without roots) is washed with running water, chopped, and extracted with water for 3 hours at a solid-liquid ratio of 1:9, and then allowed to stand; the filtrate is filtered; and then rotary evaporated until the solvent evaporates, and freeze-dried to obtain the yarrow extract.

[0060] The preparation method of the barley malt extract and roselle fermentation filtrate is as follows:

[0061] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0062] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0063] The components of the fermentation broth are:

[0064] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0065] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0066] The inoculation amount is: 6 vol%.

[0067] Fermentation conditions were: 30°C, 24h.

[0068] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0069] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0070] Example 3

[0071] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0072] The mass ratio is: 10:0.02:2::0.5:2.5:0.31.

[0073] The preparation method of the yarrow extract is as follows:

[0074] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution in a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continued to be added until the solid-liquid ratio is 1:9, and then poured into a container. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand; the filtrate is filtered; the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0075] The preparation method of the barley malt extract and roselle fermentation filtrate is as follows:

[0076] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0077] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0078] The components of the fermentation broth are:

[0079] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0080] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0081] The inoculation amount is: 6 vol%.

[0082] Fermentation conditions were: 30°C, 24h.

[0083] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0084] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0085] Example 4

[0086] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0087] The mass ratio is: 10:0.02:0.5:2.5:0.31.

[0088] The preparation method of the yarrow extract is as follows:

[0089] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0090] The preparation method of the barley malt extract is as follows:

[0091] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0092] (2) After soaking, draining the water, germinating the barley in a germination device for 4-6 days, taking it out, chopping it, and fermenting it; after fermentation, filtering it, taking the filtrate and evaporating it at a constant temperature to remove part of the solvent, and thus obtaining the barley malt extract.

[0093] The components of the fermentation broth are:

[0094] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0095] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0096] The inoculation amount is: 6 vol%.

[0097] Fermentation conditions were: 30°C, 24h.

[0098] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0099] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0100] Example 5

[0101] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0102] The mass ratio is: 10:0.02:2::0.5:2.5:0.31.

[0103] The preparation method of the yarrow extract is as follows:

[0104] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0105] The preparation method of the barley malt extract is as follows:

[0106] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0107] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0108] The components of the fermentation broth are:

[0109] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0110] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0111] The inoculation amount is: 6 vol%.

[0112] Fermentation conditions were: 30°C, 24h.

[0113] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0114] The preparation method of the Roselle fermentation filtrate is as follows:

[0115] Wash and chop the roselle and then ferment it; filter it after fermentation, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the roselle fermentation filtrate.

[0116] The components of the fermentation broth are:

[0117] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0118] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae 31936; the mass ratio of inoculum volume is 2:1.

[0119] The inoculation amount is: 3 vol%.

[0120] Fermentation conditions were: 30°C, 24h.

[0121] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0122] Example 6

[0123] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract, 1-methylhydantoin-2-imide and diglucosyl gallic acid;

[0124] The mass ratio is: 10:0.02:2::0.5:2.5:0.31.

[0125] The preparation method of the yarrow extract is as follows:

[0126] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0127] The preparation method of the barley malt extract and roselle fermentation filtrate is as follows:

[0128] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0129] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0130] The components of the fermentation broth are:

[0131] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0132] The fermentation strain is: Saccharomyces cerevisiae 31936.

[0133] The inoculation amount is: 6 vol%.

[0134] Fermentation conditions were: 30°C, 24h.

[0135] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0136] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0137] Example 7

[0138] This embodiment provides a whitening composition containing yarrow, the composition comprising: yarrow extract, barley malt extract, roselle fermentation liquid, Japanese callicarpa extract;

[0139] The mass ratio is: 10:0.02:2::0.5.

[0140] The preparation method of the yarrow extract is as follows:

[0141] Freshly picked yarrow (with roots) is washed with running water, immersed in a methanol solution at a solid-liquid ratio of 1:1, and irradiated with light for 24 hours. The roots are removed and methanol is continuously added until the solid-liquid ratio is 1:9. The solution is poured into a container, and 1 wt% disodium EDTA of yarrow is added. The yarrow in the methanol is then chopped with a knife for 3 hours and allowed to stand. The filtrate is filtered and the filtrate is then rotary evaporated until the methanol evaporates, and freeze-dried to obtain the yarrow extract.

[0142] The preparation method of the barley malt extract and roselle fermentation filtrate is as follows:

[0143] (1) Soak the barley in water for 24-36 hours, control the temperature at 20±3℃, and keep the air circulating to keep it moist;

[0144] (2) After soaking, drain the water and germinate the barley in a germination device for 4-6 days, then take it out, chop it and mix it with the chopped roselle to ferment it; after fermentation, filter it, take the filtrate and evaporate it at a constant temperature to remove part of the solvent, so as to obtain the barley malt extract.

[0145] The components of the fermentation broth are:

[0146] Casein peptone (trypsin digestion) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, distilled water 1000.0 mL.

[0147] Fermentation strain: Bacillus licheniformis 24407 and Saccharomyces cerevisiae The mass ratio of inoculum was 2:1.

[0148] The inoculation amount is: 6 vol%.

[0149] Fermentation conditions were: 30°C, 24h.

[0150] The mass ratio of barley malt to culture solution during fermentation is 1:10.

[0151] This embodiment provides a whitening and spot-lightening skin care lotion, which comprises: 10 parts of a whitening and spot-lightening skin care composition, 0.16 parts of carbomer, 0.5 parts of glyceryl stearate, 1.3 parts of cetearyl alcohol, 0.2 parts of glycerol, 2.6 parts of glycerol polyether, and water to 100 parts. The specific preparation method is to mix the whitening and spot-lightening skin care composition, water, carbomer, and glycerol evenly, and then add the remaining raw materials and stir evenly.

[0152] Performance Testing

[0153] Test 1: The emulsions prepared in Examples 1 to 7 were placed at 5°C, 25°C, and 40°C for 90 days to check their properties. The test results are shown in Table 1.

[0154] Table 1 Test results

[0155]

[0156] Test 2: Whitening efficacy test

[0157] Test method: 70 volunteers with darker facial complexion were screened and randomly divided into 7 groups, 10 in each group. Before trying the sample, the melanin MI data of the face was collected using a skin red-melanin tester and a test probe (Mexameter MX18, CK, Germany), and the data was used as the skin background value. The trial subjects used the composition once in the morning and evening, and the data was collected again after 4 consecutive weeks of use. The changes in the melanin content of the skin of the subjects before and after trying the composition were evaluated to determine the whitening effect. The corresponding test results are shown in Table 2 below.

[0158] Among them, the melanin content change rate △MI% = (Tn-T0) / T0, and the final result is the average value; the higher the △MI% reduction value, the better the whitening effect.

[0159] Wherein, Tn is the melanin content value of the test area after 4 weeks of continuous use; T0 is the initial value of the melanin content of the test area.

[0160] Table 2 Test results

[0161] Example △MI% Example 1 -18.46 Example 2 -14.21 Example 3 -17.35 Example 4 -13.85 Example 5 -15.37 Example 6 -16.52 Example 7 -10.32

[0162] Test 3: Effects of the compositions of Examples 1-7 and arbutin on tyrosinase activity in B16-F10 cells

[0163] The effects of the compositions of Examples 1-7 and arbutin at the same concentration on the tyrosinase activity of B16-F10 cells were studied by cell tyrosinase activity experiments.

[0164] Experimental method: B16-F10 cells in the logarithmic growth phase were digested and counted, and the cells were diluted with DMEM complete medium and inoculated into well plates at a density of 8×10 3 / well. Culture in an incubator at 37°C, 5% CO2 for 24 hours. Discard the original culture medium and wash twice with PBS. The blank control group (control) only added DMEM, and the sample group (sample) added an equal volume of DMEM containing the composition of Example 1-7 or arbutin, and the final concentration of the sample was 100μM. After culturing in a cell culture incubator for 48 hours, discard the culture medium, wash twice with PBS and discard the culture medium, add 90μL of 1% Triton X100 solution, and place at -80°C for 30 minutes. Then take it out and dissolve it at 37°C. After dissolution, add 10μL of L-Dopa with a final concentration of 0.25mg / mL, and continue to incubate at 37°C for 30 minutes. Finally, use an enzyme reader to measure the absorbance at 475nm.

[0165] Tyrosinase inhibition rate (%) = (1-sample absorbance / control absorbance) × 100%

[0166] SPSS.25 was used for statistical analysis, and the results are shown in Table 3.

[0167] Table 3 Test results

[0168] Example Inhibition rate % Example 1 29.83±1.34 Example 2 24.12±2.31 Example 3 28.31±1.76 Example 4 21.12±1.31 Example 5 26.21±2.53 Example 6 27.46±3.53 Example 7 19.64±3.34 Arbutin 15.53±1.32

[0169] Test 4: Human safety test items

[0170] Materials and methods

[0171] 1. Test substance: emulsion of Example 1.

[0172] 2. Negative control: blank control.

[0173] 3. Subjects: A total of 30 people, including 12 males and 18 females, aged 19 to 33 years old, with an average age of 22.1±2.6 years old, who met the voluntary selection criteria for subjects.

[0174] 4. Patch test method: Select qualified patch test equipment and use the closed patch test method. Place about 0.020 of the test substance in the patch tester and apply it to the back of the subject with a low-allergenic tape. Remove the test substance after 24 hours and observe the skin reaction at 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0175] Table 4 Test results

[0176]

[0177]

[0178] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A whitening composition containing yarrow, characterized in that: The composition comprises: yarrow extract and barley malt extract.

2. The whitening composition containing yarrow according to claim 1, characterized in that The mass ratio of the yarrow extract to the barley malt extract is 10:(0.01-1).

3. The whitening composition containing yarrow according to claim 1, characterized in that The mass ratio of the yarrow extract to the barley malt extract is 10:0.

02.

4. The whitening composition containing yarrow according to claim 1, characterized in that The preparation method of the barley malt extract is to ferment and extract barley malt to obtain the barley malt extract.

5. The whitening composition containing yarrow according to claim 1, characterized in that: The yarrow-containing whitening composition further comprises roselle fermentation liquid.

6. The whitening composition containing yarrow according to claim 1, characterized in that: The yarrow-containing whitening composition further comprises a Japanese callicarpa extract.

7. A whitening and spot-lightening skin care composition, characterized in that: The preparation raw materials include the whitening composition containing yarrow as claimed in any one of claims 1 to 6.

8. The whitening and spot-lightening skin care composition according to claim 7, characterized in that: The composition is in the form of water, lotion, cream, paste or gel.

9. The whitening and spot-lightening skin care composition according to claim 7, characterized in that: The whitening and spot-lightening skin care composition further comprises 1-methylhydantoin-2-imide and diglucosyl gallic acid.

10. The whitening and spot-lightening skin care composition according to claim 9, characterized in that: The mass ratio of the 1-methylhydantoin-2-imide to diglucosyl gallic acid is (7-10):1.

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