A device and process for producing elastin peptide using membrane filtration separation technology

Abstract

The invention discloses an elastin peptide production device using a membrane filtration separation process, belonging to the technical field of protein peptide production, and comprising: a casing, a separation chamber is arranged at the center of the casing, and the separation chamber is used for membrane filtration of emulsion protein peptide; a plurality of membrane filtration units are arranged in sequence up and down; a central column is vertically inserted in the center of the separation chamber, and a cavity structure is arranged in the central column; a heater is installed in the central column; a feed seat is fixed on the upper side of the separation chamber, and a feed pipe is obliquely connected above the feed seat; a material guide mechanism is arranged one by one corresponding to each of the membrane filtration units, and the material guide mechanism is located above the membrane filtration unit; a discharge port is distributed on the casing and is located below the separation chamber; the invention can evenly spread the emulsion protein peptide along the radial direction of the membrane filtration unit, thereby improving the membrane separation efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of protein peptide production, and in particular relates to an elastin peptide production device and process using a membrane filtration separation process. Background Art

[0002] Elastin peptides are a type of polypeptide extracted from elastin, which are mainly found in animal tissues, such as skin, blood vessel walls, lungs, etc. Common sources include pig skin, cow skin, fish skin, etc.; the production process of elastin peptides in the existing technology mainly includes slurry adjustment, enzymatic hydrolysis, enzyme inactivation, membrane separation, ceramic membrane filtration, vacuum concentration, etc. Among them, the main purpose of membrane separation is to effectively remove large molecular impurities in the solution, such as incompletely enzymatically hydrolyzed proteins, cell fragments, etc. However, most membrane separation equipment simply pours the protein peptide solution into the machine. The protein peptide in the emulsion state is very easy to deposit on the membrane surface and has weak fluidity, which directly leads to low filtration efficiency; although most equipment is equipped with an extrusion unit to assist in the extrusion filtration of the protein peptide on the membrane surface, this can easily cause the pore size of the filter membrane to become larger or even damaged.

[0003] Therefore, it is necessary to provide an elastin peptide production device and process using a membrane filtration separation process to solve the problems raised in the above background technology. Summary of the invention

[0004] To achieve the above object, the present invention provides the following technical solution: an elastin peptide production device using a membrane filtration separation process, comprising:

[0005] The casing has a separation chamber disposed in the center thereof, and the separation chamber is used for membrane filtration of the emulsion protein peptide;

[0006] The membrane filtration units are arranged in sequence up and down, and each of the membrane filtration units is horizontally arranged in the separation chamber;

[0007] A central column, vertically inserted in the center of the separation chamber, wherein the interior of the central column is a hollow structure;

[0008] A heater, installed inside the central column, for thermostatically regulating the internal temperature of the separation chamber;

[0009] A feed seat is fixed on the upper side of the separation chamber, a feed pipe is obliquely connected to the upper side of the feed seat, and a hopper is arranged on the feed pipe;

[0010] A material guiding mechanism is provided corresponding to each of the membrane filtration units, and each of the material guiding mechanisms is located above the membrane filtration units. The material guiding mechanism evenly spreads the emulsion protein peptide along the radial direction of the membrane filtration units so that the emulsion protein peptide quickly passes through the membrane filtration units for separation and filtration;

[0011] A discharge port, distributed on the housing and located below the separation chamber;

[0012] On the outer wall of the separation chamber, a plurality of discharge channels are arranged at the horizontal end surface of each membrane filtration unit and extend tangentially along its circumference, one of the discharge channels is connected to a main pipe, and the remaining discharge channels are connected to branch pipes, and the other end of each branch pipe is connected to the main pipe; the other end of the main pipe is connected to the membrane filtration unit adjacent to it, and the uppermost main pipe is connected to the feed seat.

[0013] Further, as a preference, the material guiding mechanism comprises:

[0014] A shaft tube, sleeved and fixed on the central column, and the central column is rotatably connected in the casing;

[0015] A ring frame is coaxially arranged outside the shaft tube, and a plurality of connecting rods are arranged on the inner circumference of the ring frame, and the other ends of the connecting rods are connected to the shaft tube;

[0016] A vortex disk is coaxially fixed on the shaft tube, and a plurality of flow holes are distributed circumferentially on the vortex disk;

[0017] A material guide plate is arranged corresponding to each of the connecting rods and is fixed on the connecting rods in parallel;

[0018] The gear plate is coaxially fixed to the upper end of the central column. A driving part is installed outside the housing. The output end of the driving part is connected to the gear plate through a transmission chain for transmission.

[0019] Further, as a preference, a plurality of bucket plates are arranged in the casing, the bucket plates are fixed in the casing and located below the membrane filtration unit, and the bucket plates are rotatably connected to the center column; a plurality of through holes are opened on the inner circumference of the bucket plates, and each of the through holes is vertically distributed above the vortex disk.

[0020] Further, as a preference, the connecting rod is rotatably connected between the shaft tube and the ring frame, the ends of the connecting rod are located in the center column and are fixed with bevel gears, an inner rod is rotatably connected in the center column, a bevel gear is sleeved on the inner rod, the bevel gears are meshed with the bevel gears for transmission, a motor is installed on the center column, and the output end of the motor is connected to the inner rod;

[0021] The guide plate can contact the surface of the membrane filtration unit horizontally or obliquely during the rotation adjustment along with the connecting rod.

[0022] Further, preferably, the inclination angle of the guide plate is not greater than 70°.

[0023] Further, as a preference, the membrane filtration unit comprises a microfiltration membrane, which is horizontally arranged in the separation chamber, and the microfiltration membranes in each of the membrane filtration units are arranged in order from large to small meshes from top to bottom;

[0024] A plurality of fixed lining plates are distributed circumferentially on the microfiltration membrane, and a plurality of ultrasonic vibrators corresponding to the fixed lining plates are arranged in the separation chamber, and the output end of each ultrasonic vibrator is connected to the fixed lining plate.

[0025] Further, as a preference, the two ultrasonic vibrators in opposite directions work synchronously, and the working states of the two ultrasonic vibrators located at adjacent positions are set oppositely.

[0026] Further, as a preferred method, a process for producing elastin peptide using a membrane filtration separation process comprises the following steps:

[0027] S1. Raw material extraction, crushing and dissolving the selected protein raw material, and adding a solvent to promote the dissolution effect; preliminarily filtering the dissolved solution and removing large particle impurities;

[0028] S2. Enzymatic hydrolysis of proteins: selecting appropriate proteases and performing enzymatic hydrolysis under suitable temperature and acid-base conditions;

[0029] S3. Membrane filtration separation, the enzymatically hydrolyzed emulsion protein peptide is fed into the feed seat through the hopper, and the emulsion protein peptide flows through each membrane filtration unit in turn, wherein the protein peptide can flow vertically to the vortex disk through the through hole in the hopper plate, so that the vortex disk cooperates with the guide plate to transfer the material under continuous rotation, so that the protein peptide flows and spreads evenly on the surface of the microfiltration membrane, and the ultrasonic vibrator provides vibration to the microfiltration membrane to shake the material to maintain the filtration efficiency;

[0030] S4. Reflux cycle, the protein peptides at the edge of the microfiltration membrane can enter the discharge channel and flow into the main pipe through the branch pipes of each discharge channel. After entering the main pipe, the protein peptides recirculate and flow into the bucket plate located above, thereby achieving continuous circulation flow;

[0031] S5. Concentration treatment: The filtered and separated protein peptides are evaporated and concentrated, and then dried by a spray dryer to obtain powdered elastin peptides.

[0032] Compared with the prior art, the present invention has the following beneficial effects:

[0033] In the present invention, the emulsion protein peptides after enzymatic hydrolysis can flow downward through the through holes in the bucket plate in sequence and fall onto the surface of the vortex disk in the material guide mechanism. At this time, the vortex disk can cooperate with the material guide plate to evenly spread the emulsion protein peptides along the radial direction of the membrane filtration unit during continuous rotation, thereby improving the membrane separation efficiency; and some unfiltered protein peptides can also enter the main pipe from the discharge channel and recirculate into the bucket plate located above, thereby maintaining the fluidity of the protein peptides and avoiding excessive accumulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 It is a structural schematic diagram of the discharge channel in the present invention;

[0036] Figure 3 It is a structural schematic diagram of the material guiding mechanism in the present invention;

[0037] Figure 4 It is a structural schematic diagram of the bucket plate and the driving part in the present invention;

[0038] Figure 5 is a cross-sectional view of the central column in the present invention;

[0039] Figure 6 for Figure 1 A schematic diagram of the structure enlargement in the middle;

[0040] In the figure: 1. casing; 11. separation chamber; 12. center column; 13. heater; 14. feed seat; 15. hopper; 16. discharge port; 2. membrane filtration unit; 21. microfiltration membrane; 22. fixed lining plate; 23. ultrasonic vibrator; 3. material guide mechanism; 31. shaft tube; 32. ring frame; 33. connecting rod; 34. eddy current disk; 35. flow hole; 36. material guide plate; 37. gear plate; 38. driving part; 4. discharge channel; 41. main pipe; 42. branch pipe; 5. bucket plate; 51. through hole; 6. inner rod; 61. bevel gear; 62. motor. DETAILED DESCRIPTION

[0041] See also Figure 1-Figure 6 In an embodiment of the present invention, a device for producing elastin peptides using a membrane filtration separation process comprises:

[0042] The casing 1 has a separation chamber 11 disposed in the center thereof, and the separation chamber 11 is used for membrane filtration of the emulsion protein peptide;

[0043] The membrane filtration units 2 are arranged in sequence up and down, and each of the membrane filtration units 2 is horizontally arranged in the separation chamber 11;

[0044] A central column 12 is vertically inserted in the center of the separation chamber 11, and the interior of the central column 12 is a hollow structure;

[0045] The heater 13 is installed inside the central column 12 and is used to thermostatically regulate the internal temperature of the separation chamber 11. By precisely controlling the temperature, the protein peptide can be prevented from being denatured or degraded due to excessively high or low temperatures, thereby ensuring its biological activity.

[0046] A feed seat 14 is fixed on the upper side of the separation chamber 11, and a feed pipe is obliquely connected to the upper side of the feed seat 14, and a hopper 15 is arranged on the feed pipe;

[0047] A material guiding mechanism 3 is provided corresponding to each of the membrane filtration units 2. The material guiding mechanism 3 is located above the membrane filtration unit 2. The material guiding mechanism 3 spreads the emulsion protein peptide along the radial direction of the membrane filtration unit 2 so that it can quickly pass through the membrane filtration unit for separation and filtration;

[0048] A discharge port 16 is disposed on the housing 1 and is located below the separation chamber 11; it is used to discharge and collect the protein peptides separated by membrane filtration;

[0049] On the outer wall of the separation chamber 11, at the horizontal end surface of each membrane filtration unit 2, a plurality of discharge channels 4 are provided along the circumference and tangentially extend, one of the discharge channels 4 is connected to a main pipe 41, and the remaining discharge channels 4 are connected to branch pipes 42, and the other end of each branch pipe 42 is connected to the main pipe 41; the other end of the main pipe 41 is connected to the adjacent upper membrane filtration unit 2, and the uppermost main pipe 41 is connected to the feed seat 14; that is, the protein peptide can flow evenly along the radial direction of the membrane filtration unit 2, ensuring the filtration efficiency. , and the protein peptides at the edge of the membrane filtration unit 2 can flow into each discharge channel 4 accordingly, and then enter the main pipe 41 through the branch pipe 42 on the discharge channel 4 for collection (a pumping pump can be used to provide continuous conveying power), and the protein peptides in the main pipe 41 are recirculated and discharged, thereby realizing continuous flow filtration of the protein peptides. Compared with the membrane filtration or filter press equipment in the traditional technology, the present invention can reduce the resistance of the protein peptides during membrane filtration, increase the filtration flow rate, and avoid the clogging of the membrane surface caused by excessive accumulation of protein peptides, and the destructiveness to the membrane is almost zero, thereby extending the service life of the membrane.

[0050] In this embodiment, the material guiding mechanism 3 includes:

[0051] The shaft tube 31 is sleeved and fixed on the central column 12, and the central column 12 is rotatably connected in the housing 1;

[0052] A ring frame 32 is coaxially arranged outside the shaft tube 31, and a plurality of connecting rods 33 are arranged on the inner circumference of the ring frame 32, and the other ends of the connecting rods 33 are connected to the shaft tube 31;

[0053] The vortex disk 34 is coaxially fixed on the shaft tube 31, and a plurality of flow holes 35 are distributed circumferentially on the vortex disk 34; the vortex disk 34 can guide the protein peptide to flow away from the center of the circle during continuous rotation with the central column;

[0054] The guide plates 36 are arranged one by one corresponding to each of the connecting rods 33 and are fixed on the connecting rods 33 in parallel;

[0055] The gear plate 37 is coaxially fixed to the upper end of the central column 12. A driving unit 38 is installed outside the casing 1. The output end of the driving unit 38 is connected to the gear plate 37 through a transmission chain, thereby driving the vortex plates 34 on the central column 12 to rotate and guide the material, thereby ensuring the flow filtration strength of the protein peptide.

[0056] As a preferred embodiment, a plurality of hopper plates 5 are arranged in the casing 1, the hopper plates 5 are fixed in the casing 1 and are located below the membrane filtration unit 2, and the hopper plates 5 are rotatably connected to the center column 12; a plurality of through holes 51 are opened on the inner circumference of the hopper plates 5, and each of the through holes 51 is vertically distributed above the vortex disk 34, so that the protein peptides can be directly transported to the surface of the vortex disk 34 through the through holes 51, avoiding the flow impact of the protein peptides on the microfiltration membrane 21 and extending its service life; it should be noted that the main pipe 41 on the discharge channel 4 at the corresponding position is connected to the hopper plates 5, so that the continuous circulation of the protein peptides can be achieved to avoid excessive accumulation on the membrane surface to cause blockage.

[0057] In this embodiment, the connecting rod 33 is rotatably connected between the shaft tube 31 and the ring frame 32, and the ends of the connecting rod 33 are fixed with bevel gears in the center column 12. The center column 12 is rotatably connected with an inner rod 6, and a bevel gear 61 is sleeved on the inner rod 6. The bevel gears are meshed with the bevel gear 61 for transmission. A motor 62 is installed on the center column 12, and the output end of the motor 62 is connected to the inner rod 6.

[0058] The guide plate 36 can be in horizontal or inclined contact with the surface of the membrane filtration unit 2 during the rotation adjustment along with the connecting rod 33, so as to specifically adjust the guide conveying intensity according to the consistency of the protein peptide. For example, when the consistency of the protein peptide is high, the inner rod 6 is used to rotate and adjust each connecting rod 33 so that the guide plate 36 is at the maximum inclination angle, the protein peptide stays on the membrane surface for a short time, and the probability of clogging is small, thereby ensuring continuous flow filtration of the protein peptide.

[0059] In this embodiment, the inclination angle of the guide plate 36 is not greater than 70°.

[0060] In this embodiment, the membrane filtration unit 2 includes a microfiltration membrane 21, which is horizontally arranged in the separation chamber 11. The microfiltration membranes 21 in each of the membrane filtration units 2 are arranged in order from large to small meshes from top to bottom; wherein microfiltration membranes (pore size 0.1-1.0 μm), ultrafiltration membranes (pore size 1-100 kDa) and nanofiltration membranes (pore size 0.1-1 nm) can also be used in order from top to bottom;

[0061] A plurality of fixed lining plates 22 are distributed circumferentially on the microfiltration membrane 21 , and a plurality of ultrasonic vibrators 23 corresponding to the fixed lining plates 22 are arranged in the separation chamber 11 , and the output end of each ultrasonic vibrator 23 is connected to the fixed lining plate 22 .

[0062] As a preferred embodiment, the two ultrasonic vibrators 23 in opposite directions work synchronously, and the working states of the two ultrasonic vibrators 23 located at adjacent positions are set oppositely, which can effectively improve the ultrasonic vibration effect on the microfiltration membrane and further improve the filtration flowability of protein peptides.

[0063] In this embodiment, a process for producing elastin peptide using a membrane filtration separation process comprises the following steps:

[0064] S1. Raw material extraction, crushing and dissolving the selected protein raw material, and adding a solvent to promote the dissolution effect; preliminarily filtering the dissolved solution and removing large particle impurities;

[0065] S2. protein enzymatic hydrolysis, selecting suitable proteases, performing enzymatic hydrolysis under suitable temperature and acid-base conditions, and forming a protein peptide emulsion after protein enzymatic hydrolysis;

[0066] S3. Membrane filtration separation, the enzymatically hydrolyzed emulsion protein peptide is fed into the feed seat 14 through the hopper 15, and the emulsion protein peptide flows through each membrane filtration unit 2 in sequence, wherein the protein peptide can flow vertically to the vortex disk 34 through the through hole 51 in the hopper plate 5, so that the vortex disk 34 cooperates with the guide plate 36 to transfer the material under continuous rotation, so that the protein peptide flows and is evenly spread on the surface of the microfiltration membrane 21, and the ultrasonic vibrator 23 provides vibration shaking to the microfiltration membrane 21 to maintain the filtration efficiency;

[0067] S4. Reflux circulation, the protein peptides at the edge of the microfiltration membrane 21 can enter the discharge channel 4, and flow into the main pipe 41 through the branch pipes 42 of each discharge channel 4, and the protein peptides enter the main pipe 41 and recirculate into the bucket plate 5 located above, thereby achieving continuous circulation flow; avoiding the accumulation and blockage of protein peptides on the surface of the microfiltration membrane, and being able to improve the filtration flowability of protein peptides to a certain extent;

[0068] S5. Concentration treatment: The filtered and separated protein peptides are evaporated and concentrated, and then dried by a spray dryer to obtain powdered elastin peptides.

[0069] What has been described above are only preferred specific implementations of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, can make equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An elastin peptide production device using a membrane filtration separation process, characterized in that: It includes: The casing has a separation chamber disposed in the center thereof, and the separation chamber is used for membrane filtration of the emulsion protein peptide; The membrane filtration units are arranged in sequence up and down, and each of the membrane filtration units is horizontally arranged in the separation chamber; the central column is vertically inserted in the center of the separation chamber, and the central column is set as a cavity structure; A heater, installed inside the central column, for thermostatically regulating the internal temperature of the separation chamber; A feed seat is fixed on the upper side of the separation chamber, a feed pipe is obliquely connected to the upper side of the feed seat, and a hopper is arranged on the feed pipe; A material guiding mechanism is provided corresponding to each of the membrane filtration units, and each of the material guiding mechanisms is located above the membrane filtration units. The material guiding mechanism evenly spreads the emulsion protein peptide along the radial direction of the membrane filtration units so that the emulsion protein peptide quickly passes through the membrane filtration units for separation and filtration; A discharge port, distributed on the housing and located below the separation chamber; On the outer wall of the separation chamber, at the horizontal end surface of each membrane filtration unit, a plurality of discharge channels are provided along the circumference and tangentially extend, one of the discharge channels is connected to a main pipe, and the other discharge channels are connected to branch pipes, and the other end of each branch pipe is connected to the main pipe; the other end of the main pipe is connected to the membrane filtration unit above it, and the uppermost main pipe is connected to the feed seat; The material guiding mechanism comprises: A shaft tube, sleeved and fixed on the central column, and the central column is rotatably connected in the casing; A ring frame is coaxially arranged outside the shaft tube, and a plurality of connecting rods are circumferentially connected to the ring frame, and the other ends of the connecting rods are connected to the shaft tube; A vortex disk is coaxially fixed on the shaft tube, and a plurality of flow holes are distributed circumferentially on the vortex disk; A material guide plate is arranged corresponding to each of the connecting rods and fixed parallel to the connecting rods; the material guide plate can contact the surface of the membrane filtration unit horizontally or obliquely during the rotation adjustment of the connecting rods; The gear plate is coaxially fixed to the upper end of the central column. A driving part is installed outside the housing. The output end of the driving part is connected to the gear plate through a transmission chain for transmission.

2. The elastin peptide production device using membrane filtration separation technology according to claim 1, characterized in that: A plurality of bucket plates are arranged in the casing, the bucket plates are fixed in the casing and located below the membrane filtration unit, and the bucket plates are rotatably connected to the center column; a plurality of through holes are opened on the inner circumference of the bucket plates, and each of the through holes is vertically distributed above the vortex disk.

3. The elastin peptide production device using membrane filtration separation technology according to claim 1, characterized in that: The connecting rod is rotatably connected between the shaft tube and the ring frame, and the ends of the connecting rod are fixed with bevel gears in the center column. An inner rod is rotatably connected in the center column, and a bevel gear is sleeved on the inner rod. The bevel gears are meshed with the bevel gears for transmission. A motor is installed on the center column, and the output end of the motor is connected to the inner rod.

4. The elastin peptide production device using membrane filtration separation technology according to claim 3, characterized in that: The inclination angle of the guide plate is not greater than 70°.

5. The elastin peptide production device using membrane filtration separation technology according to claim 2, characterized in that: The membrane filtration unit comprises a microfiltration membrane, which is horizontally arranged in the separation chamber, and the microfiltration membranes in each of the membrane filtration units are arranged in order from large to small meshes from top to bottom; A plurality of fixed lining plates are distributed circumferentially on the microfiltration membrane, and a plurality of ultrasonic vibrators corresponding to the fixed lining plates are arranged in the separation chamber, and the output end of each ultrasonic vibrator is connected to the fixed lining plate.

6. The elastin peptide production device using membrane filtration separation technology according to claim 5, characterized in that: The two ultrasonic vibrators in opposite directions work synchronously, and the working states of the two ultrasonic vibrators in adjacent positions are set oppositely.

7. A process for producing elastin peptides by membrane filtration separation process, which uses an elastin peptide production device by membrane filtration separation process as claimed in any one of claims 5 to 6, characterized in that: It includes the following steps: S1. Raw material extraction, crushing and dissolving the selected protein raw material, and adding a solvent to promote the dissolution effect; preliminarily filtering the dissolved solution and removing large particle impurities; S2. Enzymatic hydrolysis of proteins: selecting appropriate proteases and performing enzymatic hydrolysis under suitable temperature and acid-base conditions; S3. Membrane filtration separation, the enzymatically hydrolyzed emulsion protein peptide is fed into the feed seat through the hopper, and the emulsion protein peptide flows through each membrane filtration unit in turn, wherein the protein peptide can flow vertically to the vortex disk through the through hole in the hopper plate, so that the vortex disk cooperates with the guide plate to transfer the material under continuous rotation, so that the protein peptide flows and spreads evenly on the surface of the microfiltration membrane, and the ultrasonic vibrator provides vibration to the microfiltration membrane to shake the material to maintain the filtration efficiency; S4. Reflux cycle, the protein peptides at the edge of the microfiltration membrane can enter the discharge channel and flow into the main pipe through the branch pipes of each discharge channel. After entering the main pipe, the protein peptides recirculate and flow into the bucket plate located above, thereby achieving continuous circulation flow; S5. Concentration treatment: The filtered and separated protein peptides are evaporated and concentrated, and then dried by a spray dryer to obtain powdered elastin peptides.

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