Cell separation and extraction device

Abstract

The invention provides a cell separation and extraction device which comprises a magnet unit, the magnet unit comprises a first magnet assembly and a second magnet assembly, the first magnet assembly and the second magnet assembly are oppositely arranged in a spaced mode in the left-right direction of the cell separation and extraction device, and the first magnet assembly at least comprises a first magnet block, a second magnet block and a third magnet block; the first magnetic block, the second magnetic block and the third magnetic block are linearly and sequentially arranged in the front-back direction of the cell separation and extraction device, the first magnetic block, the second magnetic block and the third magnetic block form a Halbach array, the magnetic induction line direction in the second magnetic block points to the second magnet assembly, and the second magnet assembly at least comprises a fourth magnetic block, a fifth magnetic block and a sixth magnetic block; the fourth magnetic block, the fifth magnetic block and the sixth magnetic block are linearly and sequentially arranged in the front-back direction of the cell separation and extraction device, the fourth magnetic block, the fifth magnetic block and the sixth magnetic block form a Halbach array, and the direction of a magnetic induction line in the fifth magnetic block is the same as that of a magnetic induction line in the second magnetic block.

Description

Technical Field

[0001] The present application belongs to the technical field of cell therapy, and in particular relates to a cell separation and extraction device. Background Art

[0002] Currently, the magnetic field strength in the working area of ​​the cell separation and extraction device is low, resulting in a low recovery rate of magnetic beads in the sorting column, causing waste. Summary of the invention

[0003] The present application aims to provide a cell separation and extraction device, which has a higher magnetic field intensity in the working area.

[0004] The embodiment of the present application provides a cell separation and extraction device for applying magnetic force to a sorting column, characterized in that it comprises a magnet unit, wherein the magnet unit comprises a first magnet assembly and a second magnet assembly,

[0005] The first magnet assembly and the second magnet assembly are arranged opposite to each other in a spaced relationship along the left-right direction of the cell separation and extraction device, and a magnetic field is formed between the first magnet assembly and the second magnet assembly.

[0006] The first magnet assembly at least includes a first magnetic block, a second magnetic block and a third magnetic block, the first magnetic block, the second magnetic block and the third magnetic block are arranged in sequence in a straight line along the front-back direction of the cell separation and extraction device, the first magnetic block, the second magnetic block and the third magnetic block constitute a Halbach array, and the direction of the magnetic flux lines inside the second magnetic block points to the second magnet assembly,

[0007] The second magnet assembly includes at least a fourth magnetic block, a fifth magnetic block and a sixth magnetic block, and the fourth magnetic block, the fifth magnetic block and the sixth magnetic block are arranged in sequence in a straight line along the front-to-back direction of the cell separation and extraction device, and the fourth magnetic block, the fifth magnetic block and the sixth magnetic block constitute a Halbach array, and the direction of the magnetic flux lines inside the fifth magnetic block is the same as the direction of the magnetic flux lines inside the second magnetic block.

[0008] In at least one possible embodiment, when viewed from the top and bottom of the cell separation and extraction device, the magnet unit is U-shaped as a whole.

[0009] The magnetic flux lines inside the first magnetic block point to the second magnetic block, the magnetic flux lines inside the third magnetic block point to the second magnetic block, the first magnetic block is close to the opening of the U shape, and the third magnetic block is close to the bottom of the U shape.

[0010] In at least one possible implementation, in the front-to-back direction, the length of the second magnetic block is greater than the length of the first magnetic block, and the length of the second magnetic block is greater than the length of the third magnetic block.

[0011] In at least one possible embodiment, when observed in the up and down direction of the cell separation and extraction device, the magnet unit is U-shaped as a whole, the direction of the magnetic flux lines inside the fourth magnetic block points away from the fifth magnetic block, the direction of the magnetic flux lines inside the sixth magnetic block points away from the fifth magnetic block, the fourth magnetic block is close to the opening of the U-shape, and the sixth magnetic block is close to the bottom of the U-shape.

[0012] In at least one possible implementation, in the front-to-back direction, the length of the fifth magnetic block is greater than the length of the fourth magnetic block, and the length of the fifth magnetic block is greater than the length of the sixth magnetic block.

[0013] In at least one possible implementation manner, the fourth magnetic block and the first magnetic block are arranged opposite to each other, the fifth magnetic block and the second magnetic block are arranged opposite to each other, and the sixth magnetic block and the third magnetic block are arranged opposite to each other.

[0014] In at least one possible embodiment, the magnet unit also includes a shielding shell. When viewed in the up and down directions of the cell separation and extraction device, the shielding shell is U-shaped as a whole. The first magnet assembly and the second magnet assembly are installed on the two arms of the U-shape of the shielding shell, and the shielding shell is made of soft magnetic material.

[0015] In at least one possible implementation manner, the shielding shell is provided with two installation grooves, the two installation grooves respectively accommodate the first magnet assembly and the second magnet assembly, and fixing plates are installed at the openings of the two installation grooves.

[0016] In at least one possible embodiment, the cell separation and extraction device further includes a housing, and the magnet unit is connected to the housing so as to be slidable along the front-rear direction relative to the housing.

[0017] In at least one possible embodiment, the cell separation and extraction device further includes a linear drive mechanism, which includes a motor, a lead screw, a guide rail, and a slider.

[0018] The lead screw is connected to the output shaft of the motor, the slider and the lead screw are threadedly matched, the guide rail extends along the front-to-back direction of the cell separation and extraction device, the slider can be slidably installed on the guide rail along the guide rail, and the magnet unit is connected to the slider, so that the motor can drive the magnet unit to move along the front-to-back direction of the cell separation and extraction device.

[0019] By adopting the above technical solution, the first magnet assembly and the second magnet assembly use magnetic blocks arranged in a Halbach array, so that fewer magnetic blocks can provide a stronger magnetic field strength, thereby increasing the recovery rate of magnetic beads in the separation column. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic structural diagram of a cell separation and extraction device according to an embodiment of the present application is shown.

[0021] Figure 2 A schematic structural diagram of a cell separation and extraction device according to an embodiment of the present application is shown from another angle.

[0022] Figure 3 A schematic structural diagram of a magnet unit of a cell separation and extraction device according to an embodiment of the present application is shown.

[0023] Figure 4 A schematic structural diagram of a magnet unit (magnet assembly and upper cover plate are not shown) of a cell separation and extraction device according to an embodiment of the present application is shown.

[0024] Figure 5 A schematic structural diagram of a magnet assembly, a shielding shell, and a fixing plate of a cell separation and extraction device according to an embodiment of the present application is shown.

[0025] Figure 6 A schematic diagram of a magnet assembly and magnetic flux lines of a cell separation and extraction device according to an embodiment of the present application is shown.

[0026] Figure 7 A schematic structural diagram of the housing of a cell separation and extraction device according to an embodiment of the present application is shown.

[0027] Figure 8 A schematic structural diagram showing another angle of the housing of the cell separation and extraction device according to an embodiment of the present application is shown.

[0028] Description of Reference Numerals

[0029] 1 housing 11 support plate mounting hole cavity 12

[0030] 2 Magnet unit

[0031] 21 Magnet assembly

[0032] 211 first magnet assembly 2111 first magnetic block 2112 second magnetic block 2113 third magnetic block

[0033] 212 second magnet assembly 2121 fourth magnet block 2122 fifth magnet block 2123 sixth magnet block

[0034] 22 shielding shell 23 upper cover plate 24 lower cover plate 25 fixing plate

[0035] 3 linear drive mechanism 31 motor 32 screw rod 33 guide rail 34 slider

[0036] X is the front-to-back direction, Y is the left-right direction, and Z is the up-down direction. DETAILED DESCRIPTION

[0037] In order to more clearly explain the above-mentioned purposes, features and advantages of the present application, the specific implementation methods of the present application are described in detail in this section in conjunction with the accompanying drawings. In addition to the various implementation methods described in this section, the present application can also be implemented in other different ways. Without violating the spirit of the present application, those skilled in the art can make corresponding improvements, deformations and substitutions, so the present application is not limited by the specific embodiments disclosed in this section. The scope of protection of the present application shall be subject to the claims.

[0038] like Figures 1 to 8 As shown, an embodiment of the present application provides a cell separation and extraction device, which includes a housing 1, a magnet unit 2 and a linear drive mechanism 3. The magnet unit 2 is slidably connected to the housing 1 along a front-rear direction X relative to the housing 1.

[0039] like Figure 1 and Figure 2 As shown, the linear drive mechanism 3 may include a motor 31, a screw rod 32, a guide rail 33 and a slider 34. The screw rod 32 is connected to the output shaft of the motor 31, the slider 34 and the screw rod 32 are threadedly matched, the guide rail 33 extends along the front-to-back direction X of the cell separation and extraction device, and the slider 34 can be slidably mounted on the guide rail 33 along the guide rail 33. The magnet unit 2 is connected to the slider 34, and the motor 31 can drive the magnet unit 2 to move along the front-to-back direction X of the cell separation and extraction device.

[0040] like Figures 6 to 8 As shown, the housing 1 may be provided with a support plate mounting hole 11, to which a sorting column support plate may be connected. The sorting column support plate is used to support and place the sorting column 100 so that the sorting column 100 is in the working area.

[0041] The housing 1 is provided with a cavity 12 which is capable of at least partially accommodating the magnet unit 2 (see Figure 2 ). When the magnet unit 2 is placed in the housing 1, the sorting column 100 can be in the magnetic field of the magnet unit 2, thereby adsorbing the magnetic beads in the sorting column 100. When the magnet unit 2 leaves the housing 1, the sorting column 100 leaves the magnetic field, and the magnetic beads in the sorting column 100 can be eluted.

[0042] like Figures 3 to 6 As shown, the magnet unit 2 includes a magnet assembly 21 , a shielding shell 22 , an upper cover plate 23 , a lower cover plate 24 and a fixing plate 25 .

[0043] like Figure 5 and Figure 6As shown, the magnet assembly 21 may include a first magnet assembly 211 and a second magnet assembly 212, both of which may be rectangular blocks, and the first magnet assembly 211 and the second magnet assembly 212 may be arranged opposite to each other at intervals along the left-right direction Y. A sorting column is placed between the first magnet assembly 211 and the second magnet assembly 212, so that the sorting column is in the magnetic field of the magnet assembly 21. Observing along the up-down direction Z, the magnet unit 2 may be U-shaped as a whole, and the first magnet assembly 211 and the second magnet assembly 212 are respectively located at the two arms of the U-shape.

[0044] Observed along the up-down direction Z, the shielding shell 22 can be U-shaped as a whole. The shielding shell 22 can have two mounting grooves 221 arranged opposite to each other, and the two mounting grooves 221 are located at two opposite arms of the U-shape. The first magnet assembly 211 and the second magnet assembly 212 are respectively arranged in the two mounting grooves 221. The upper cover plate 23 and the lower cover plate 24 are respectively connected to the two ends of the shielding shell 22 in the up-down direction Z, and the two fixing plates 25 are respectively connected to the openings of the two mounting grooves 221. The fixing plate 25 can be made of non-magnetic conductive material, and the fixing plate 25 can be made of, for example, a 1 mm thick stainless steel plate, so that it can not affect the magnetic field strength, but also has sufficient mechanical strength to fix the magnet assembly 21, and will not cause deformation of the magnet unit 2. The upper cover plate 23 and the lower cover plate 24 can be made of non-magnetic conductive materials such as stainless steel, plastic, aluminum alloy, etc. Optionally, the upper cover plate 23 and the lower cover plate 24 can be made of the same material as the fixing plate 25.

[0045] The shielding shell 22 can be made of a soft magnetic material such as iron, and the shielding shell 22 can be magnetized to form a magnetic circuit and enhance the magnetic field strength in the working area (i.e., between the first magnet assembly 211 and the second magnet assembly 212). The shielding shell 22 can also shield the magnetic field, reduce leakage magnetic flux, and make the magnetic field strength in the non-working area lower, for example, less than or equal to 50 Gauss.

[0046] like Figure 5 As shown, the first magnet assembly 211 includes at least a first magnetic block 2111, a second magnetic block 2112 and a third magnetic block 2113. The first magnetic block 2111, the second magnetic block 2112 and the third magnetic block 2113 can be arranged in sequence in a straight line along the front-back direction X of the cell separation and extraction device, and the first magnetic block 2111 and the third magnetic block 2113 are respectively arranged on both sides of the second magnetic block 2112. The first magnetic block 2111 can be close to the opening of the U shape, and the third magnetic block 2113 can be close to the bottom of the U shape. The first magnetic block 2111, the second magnetic block 2112 and the third magnetic block 2113 constitute a Halbach array.

[0047] The direction of the magnetic flux lines inside the first magnetic block 2111 may point to the second magnetic block 2112 , the direction of the magnetic flux lines inside the second magnetic block 2112 may point to the second magnet assembly 212 , and the direction of the magnetic flux lines inside the third magnetic block 2113 may point to the second magnetic block 2112 .

[0048] The second magnet assembly 212 includes at least a fourth magnetic block 2121, a fifth magnetic block 2122, and a sixth magnetic block 2123. The fourth magnetic block 2121, the fifth magnetic block 2122, and the sixth magnetic block 2123 can be arranged in sequence in a straight line along the front-back direction X of the cell separation and extraction device, and the fourth magnetic block 2121 and the sixth magnetic block 2123 are respectively arranged on both sides of the fifth magnetic block 2122, the fourth magnetic block 2121 can be close to the opening of the U-shape, and the sixth magnetic block 2123 can be close to the bottom of the U-shape. The fourth magnetic block 2121, the fifth magnetic block 2122, and the sixth magnetic block 2123 constitute a Halbach array.

[0049] The direction of the magnetic flux lines inside the fourth magnetic block 2121 can point to a direction away from the fifth magnetic block 2122, and the fourth magnetic block 2121 and the first magnetic block 2111 can be arranged relative to each other. The direction of the magnetic flux lines inside the fifth magnetic block 2122 is the same as the direction of the magnetic flux lines inside the second magnetic block 2112, and the fifth magnetic block 2122 and the second magnetic block 2112 can be arranged relative to each other. The direction of the magnetic flux lines inside the sixth magnetic block 2123 can point to a direction away from the fifth magnetic block 2122, and the sixth magnetic block 2123 and the third magnetic block 2113 can be arranged relative to each other.

[0050] The magnetic circuit formed by the first magnet assembly 211 and the second magnet assembly 212 arranged in this way can form a stronger magnetic field in the working area with fewer magnetic blocks, thereby improving the recovery rate of magnetic beads in cell sorting of the sorting column 100. For example, the magnetic field strength in the working area can be greater than 1 Tesla (i.e., 10,000 Gauss).

[0051] Furthermore, in the front-to-back direction X, the length of the second magnetic block 2112 may be greater than the length of the first magnetic block 2111 , the length of the second magnetic block 2112 may be greater than the length of the third magnetic block 2113 , and the length of the first magnetic block 2111 may be equal to the length of the third magnetic block 2113 .

[0052] Furthermore, in the front-to-back direction X, the length of the fifth magnetic block 2122 may be greater than the length of the fourth magnetic block 2121 , the length of the fifth magnetic block 2122 may be greater than the length of the sixth magnetic block 2123 , and the length of the fourth magnetic block 2121 may be equal to the length of the sixth magnetic block 2123 .

[0053] It should be understood that at least some aspects or features of the above-mentioned implementation modes, embodiments or examples may be appropriately combined.

[0054] It is understood that in the present application, when the number of parts or components is not particularly limited, the number may be one or more, and the multiple here means two or more. For the case where the number of parts or components shown in the drawings and / or described in the specification is a specific number such as two, three, four, etc., the specific number is usually exemplary and not restrictive, and it can be understood as multiple, that is, two or more, but this does not mean that the present application excludes the case of one.

[0055] In the present application, unless otherwise clearly stated or limited, the terms such as "install", "assemble", "assemble", "connect", "connect", "couple", "link", "abut", "connect", "interconnect", "intercommunication", "conduction", "fixation", "fastening" and the like should be understood in a broad sense, for example, they can be direct or indirect. For example, regarding connection, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly stated or limited. For example, regarding connectivity / conduction, it can be direct connectivity / conduction, or it can be indirect connectivity / conduction via an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0056] In the present application, unless otherwise clearly stated or limited, a component disposed on / installed on / located on / accommodated on / placed in, within, inside, etc. another component may be any of the following two situations: a part or most of the one component is located in the other component; and the one component is completely accommodated in the other component.

[0057] Although the present application is described in detail using the above-mentioned embodiments, it is obvious to those skilled in the art that the present application is not limited to the embodiments described in this specification. The present application can be modified and implemented as a modified embodiment without departing from the subject matter and scope of the present application as determined by the claims. Therefore, the description in this specification is for the purpose of illustration and does not have any restrictive meaning for the present application.

Claims

1. A cell separation and extraction device for applying magnetic force to a sorting column, characterized in that: The invention comprises a magnet unit (2), wherein the magnet unit (2) comprises a first magnet component (211) and a second magnet component (212), The first magnet assembly (211) and the second magnet assembly (212) are arranged opposite to each other at intervals along the left-right direction (Y) of the cell separation and extraction device, and a magnetic field is formed between the first magnet assembly (211) and the second magnet assembly (212). The first magnet assembly (211) comprises at least a first magnetic block (2111), a second magnetic block (2112) and a third magnetic block (2113); the first magnetic block (2111), the second magnetic block (2112) and the third magnetic block (2113) are arranged in sequence in a straight line along the front-to-back direction (X) of the cell separation and extraction device; the first magnetic block (2111), the second magnetic block (2112) and the third magnetic block (2113) form a Halbach array; the direction of the magnetic flux lines inside the second magnetic block (2112) points to the second magnet assembly (212); The second magnet assembly (212) comprises at least a fourth magnetic block (2121), a fifth magnetic block (2122) and a sixth magnetic block (2123); the fourth magnetic block (2121), the fifth magnetic block (2122) and the sixth magnetic block (2123) are arranged in sequence in a straight line along the front-to-back direction (X) of the cell separation and extraction device; the fourth magnetic block (2121), the fifth magnetic block (2122) and the sixth magnetic block (2123) form a Halbach array; the direction of the magnetic flux lines inside the fifth magnetic block (2122) is the same as the direction of the magnetic flux lines inside the second magnetic block (2112).

2. The cell separation and extraction device according to claim 1, characterized in that: When viewed along the vertical direction (Z) of the cell separation and extraction device, the magnet unit (2) is U-shaped as a whole. The direction of the magnetic flux lines inside the first magnetic block (2111) points to the second magnetic block (2112), the direction of the magnetic flux lines inside the third magnetic block (2113) points to the second magnetic block (2112), the first magnetic block (2111) is close to the opening of the U shape, and the third magnetic block (2113) is close to the bottom of the U shape.

3. The cell separation and extraction device according to claim 2, characterized in that: In the front-to-back direction (X), the length of the second magnetic block (2112) is greater than the length of the first magnetic block (2111), and the length of the second magnetic block (2112) is greater than the length of the third magnetic block (2113).

4. The cell separation and extraction device according to claim 1, characterized in that: When observed along the up-down direction (Z) of the cell separation and extraction device, the magnet unit (2) is generally U-shaped, the direction of the magnetic flux lines inside the fourth magnetic block (2121) points in the direction away from the fifth magnetic block (2122), the direction of the magnetic flux lines inside the sixth magnetic block (2123) points in the direction away from the fifth magnetic block (2122), the fourth magnetic block (2121) is close to the opening of the U-shape, and the sixth magnetic block (2123) is close to the bottom of the U-shape.

5. The cell separation and extraction device according to claim 1, characterized in that: In the front-to-back direction (X), the length of the fifth magnetic block (2122) is greater than the length of the fourth magnetic block (2121), and the length of the fifth magnetic block (2122) is greater than the length of the sixth magnetic block (2123).

6. The cell separation and extraction device according to claim 1, characterized in that: The fourth magnetic block (2121) and the first magnetic block (2111) are arranged opposite to each other, the fifth magnetic block (2122) and the second magnetic block (2112) are arranged opposite to each other, and the sixth magnetic block (2123) and the third magnetic block (2113) are arranged opposite to each other.

7. The cell separation and extraction device according to claim 1, characterized in that: The magnet unit (2) further comprises a shielding shell (22). When viewed along the up-down direction (Z) of the cell separation and extraction device, the shielding shell (22) is U-shaped as a whole. The first magnet assembly (211) and the second magnet assembly (212) are mounted on two arms of the U-shape of the shielding shell (22). The shielding shell (22) is made of a soft magnetic material.

8. The cell separation and extraction device according to claim 7, characterized in that: The shielding shell (22) is provided with two mounting grooves (221), the two mounting grooves (221) respectively accommodating the first magnet assembly (211) and the second magnet assembly (212), and a fixing plate (25) is installed at the openings of the two mounting grooves (221), and the fixing plate (25) is made of non-magnetic conductive material.

9. The cell separation and extraction device according to claim 7, characterized in that: The cell separation and extraction device further comprises a housing (1), and the magnet unit (2) is connected to the housing (1) in a manner that allows sliding along the front-rear direction (X) relative to the housing (1).

10. The cell separation and extraction device according to claim 1, characterized in that: The cell separation and extraction device further comprises a linear drive mechanism (3), wherein the linear drive mechanism (3) comprises a motor (31), a screw rod (32), a guide rail (33) and a slider (34). The screw rod (32) is connected to the output shaft of the motor (31), the slider (34) and the screw rod (32) are threadedly matched, the guide rail (33) extends along the front-to-back direction (X) of the cell separation and extraction device, the slider (34) can be slidably installed on the guide rail (33) along the guide rail (33), and the magnet unit (2) is connected to the slider (34), so that the motor (31) can drive the magnet unit (2) to move along the front-to-back direction (X) of the cell separation and extraction device.

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