Antibacterial gel for male vulva and preparation method thereof

Abstract

The invention provides antibacterial gel for male vulva, which is prepared from the following ingredients in parts by weight: 0.2 to 0.6 part of polyhexamethylene biguanide hydrochloride, 10 to 30 parts of xanthan gum, 90 to 130 parts of herba cynomorii extracts, 75 to 95 parts of lilac extracts, 100 to 150 parts of rhizoma smilacis glabrae root extracts, 50 to 90 parts of radix sophorae flavescentis root extracts, 40 to 80 parts of clausena lansium bark extracts and 180 to 250 parts of rhizoma polygoni cuspidati root extracts. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 130 to 200 parts of herba cistanche extract, 110 to 160 parts of herba epimedii extract and 90 to 130 parts of astragalus mongolicus extract. The antibacterial gel prepared by the preparation method disclosed by the invention is mild in drug property, free from toxic and side effects and very good in antibacterial and supplementing effects.

Description

Technical Field

[0001] The invention belongs to the technical field of gel preparation, and specifically relates to an antibacterial gel for male vulva and a preparation method thereof. Background Art

[0002] Gel refers to colloidal particles or polymers in a sol or solution that are interconnected under certain conditions to form a spatial network structure, in which the dispersion medium is completely contained, forming a non-flowing semi-solid substance. Gels can be divided into elastic gels and brittle gels. Elastic gels refer to gels that significantly shrink in volume after losing the dispersion medium, and expand again when the dispersion medium is reabsorbed. Brittle gels refer to gels that do not change in shape or volume when the dispersion medium is lost or reabsorbed.

[0003] Patent CN117180380A provides an antibacterial gel, including the following ingredients: H2O, propylene glycol, vaseline, glycerin, white oil, monoglyceride, fatty alcohol, A165, preservatives, 305 thickener, borneol, camphor, menthol, azone, Dictamni cortex, Sophora flavescens, Stemona tuber, Cnidium monnieri, Coptis chinensis, Phellodendron amurense, Alum, Honeysuckle, Saposhnikovia divaricata and the like. Through the precise ratio of Chinese medicine raw materials and additive ingredients, long-term use of the gel will not cause adverse reactions without hormones and other immunosuppressants, and the original disease will not relapse or worsen when the drug is suddenly stopped or the dosage is reduced too quickly, and there are no other side effects. Patent CN114344427A provides a gynecological antibacterial gel, including Sophora flavescens, Stemona tuberosa, Cnidium monnieri, Phellodendron amurense, aloe extract, glycerin, propylene glycol, sodium hyaluronate, nanosilver solution, carbomer, triethanolamine, dandelion sterol extract, honeysuckle extract, borneol, methylparaben, phenoxyethanol and water. The gel is made of Sophora flavescens and Phellodendron amurense, and directly acts on the affected area to reduce the absorption process of the drug and improve the absorption effect. Patent CN114832057B provides an antibacterial gynecological gel and its preparation method, including plant extract, aloe, astaxanthin microcapsules, gel matrix, moisturizer, purified water, etc., by reducing the possibility of antibacterial failure caused by astaxanthin inactivation, improving the stability of vitamin C ethyl ether and astaxanthin, thereby significantly improving the antibacterial effect of the gynecological gel.

[0004] In view of the fact that there are relatively few antibacterial gels for use on the male vulva in the prior art, and the fact that the existing antibacterial gels have a general effect on the male vulva, there is an urgent need to explore an antibacterial gel that can be applied to the male vulva. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing an antibacterial gel applied to male vulva, which uses Cynomorium songaricum extract, clove flower extract, Smilax glabra root extract, Sophora flavescens root extract, Phellodendron chinense bark extract, Polygonum cuspidatum root extract, Cistanche deserticola extract, Epimedium brevicornum extract and Astragalus mongoliae extract as main compound ingredients, and adds polyhexamethylene biguanide hydrochloride and xanthan gum to obtain the antibacterial gel applied to male vulva; the antibacterial gel has good antibacterial effect, high safety and no side effects.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: An antibacterial gel comprises the following components in parts by weight: 0.2-0.6 parts of polyhexamethylene biguanide hydrochloride, 10-30 parts of xanthan gum, 90-130 parts of cynomorium songaricum extract, 75-95 parts of lilac flower extract, 100-150 parts of smilax glabra root extract, 50-90 parts of sophora flavescens root extract, 40-80 parts of phellodendron chinense bark extract, 180-250 parts of polygonum cuspidatum root extract, 130-200 parts of cistanche extract, 110-160 parts of epimedium extract and 90-130 parts of astragalus membranaceus extract.

[0007] Furthermore, in parts by weight, the polyhexamethylene biguanide hydrochloride is 0.3-0.4 parts, xanthan gum is 15-25 parts, Cynomorium songaricum extract is 100-120 parts, clove flower extract is 80-90 parts, Smilax glabra root extract is 110-130 parts, Sophora flavescens root extract is 60-80 parts, Phellodendron chinense bark extract is 50-70 parts, Polygonum cuspidatum root extract is 200-230 parts, Cistanche deserticola extract is 150-180 parts, Epimedium brevicornum extract is 120-140 parts, and Astragalus membranaceus extract is 100-120 parts.

[0008] The preparation method of the antibacterial gel comprises the following steps: S1: Mix 100-120 parts of Cynomorium songaricum extract, 60-80 parts of Sophora flavescens root extract and 50-70 parts of Phellodendron chinense bark extract by weight, and stir and mix at a temperature of 40-60° C. for 30-50 minutes to obtain a mixed solution A; S2: Mix 75-95 parts of clove flower extract, 100-150 parts of Smilax glabra root extract and 130-200 parts of Cistanche deserticola extract by weight, and stir and mix at a temperature of 60-80° C. for 40-60 minutes to obtain a mixed solution B; S3: Mix 180-250 parts of Polygonum cuspidatum root extract, 110-160 parts of Epimedium extract and 90-130 parts of Astragalus membranaceus extract by weight, and stir the mixture at a temperature of 75-95°C for 20-30 minutes; then add the mixed solution A obtained in step S1, the mixed solution B obtained in step S2, 10-30 parts of xanthan gum, and 0.2-0.6 parts of polyhexamethylene biguanide hydrochloride, and continue stirring for 2-4 hours to obtain the antibacterial gel.

[0009] Furthermore, the preparation process of the Cynomorium songaricum extract in step S1 is as follows: the Cynomorium songaricum is aired, washed, dried, and crushed, and then passed through a 5-10 mesh sieve to obtain the Cynomorium songaricum powder; 10-20 parts of Cynomorium songaricum powder are taken by weight, and 80-120 parts of distilled water are added thereto, and stirred and mixed for 0.5-1h; 0.1-0.3 parts of a 0.1-0.2% potassium sulfate aqueous solution are added, stirred for 20-40min, the pH is adjusted to 7.3-8.1, and supernatant A is taken after centrifugation for 25-35min; 15-25 parts of the supernatant and 0.2-0.5 parts of neutral protease are taken by weight, and enzymolysis reaction is carried out at a temperature of 45-55°C for 1-2h; after the enzymolysis is completed, supernatant B is taken after static precipitation; supernatant B is centrifuged for 10-20min, and supernatant C is taken to obtain the Cynomorium songaricum extract.

[0010] Furthermore, the preparation process of Cynomorium songaricum extract is optimized as follows: after drying, washing, drying and crushing, Cynomorium songaricum is passed through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; 15 parts of Cynomorium songaricum powder are taken by weight, 100 parts of distilled water are added thereto, and the mixture is stirred for 0.8 hours; 0.25 parts of 0.15% potassium sulfate aqueous solution are added, and the mixture is stirred for 35 minutes, the pH is adjusted to 7.6-7.8, and supernatant A is obtained after centrifugation for 30 minutes; 20 parts of supernatant and 0.35 parts of neutral protease are taken by weight, and enzymolysis reaction is carried out at a temperature of 50°C for 1.5 hours; after the enzymolysis is completed, supernatant B is taken after standing and settling; supernatant B is centrifuged for 15 minutes, and supernatant C is taken to obtain Cynomorium songaricum extract.

[0011] Furthermore, the preparation process of the lilac extract in step S2 is as follows: by weight, 3-5 parts of nano-silicon dioxide, 4-7 parts of potassium citrate, 2-5 parts of chitosan, 0.5-1.5 parts of sodium alginate, 0.1-0.3 parts of vinyltriethoxysilane, 10-30 parts of n-butanol and 50-100 parts of distilled water are mixed, and stirred at a temperature of 50-60°C for 1-3h to obtain a modifier; the lilac flowers are frozen and ground into lilac powder; 5-10 parts of lilac powder and 30-60 parts of the modifier are stirred and mixed at a temperature of 40-50°C for 25-35min, and then 0.2-0.4 parts of cellulase are added, and enzymolysis is carried out at a temperature of 35-45°C for 1-2h; after centrifugation at 4000-5000r / min for 25-35min, the supernatant is taken to obtain the lilac extract.

[0012] Furthermore, the preparation process of lilac flower extract is optimized as follows: by weight, 4 parts of nano-silicon dioxide, 6 parts of potassium citrate, 3 parts of chitosan, 1 part of sodium alginate, 0.2 parts of vinyltriethoxysilane, 25 parts of n-butanol and 80 parts of distilled water are mixed, and stirred at a temperature of 55°C for 1.5 hours to obtain a modifier; the lilac flowers are frozen and ground into lilac flower powder; 8 parts of lilac flower powder and 45 parts of the modifier are stirred and mixed at a temperature of 45°C for 30 minutes, and then 0.35 parts of cellulase are added, and enzymolysis is carried out at a temperature of 40°C for 1.5 hours; after centrifugation at 7000r / min for 30 minutes, the supernatant is taken to obtain the lilac flower extract.

[0013] Furthermore, the preparation process of the Polygonum cuspidatum root extract in step S3 is as follows: slice, crush and pass the washed and dried Polygonum cuspidatum root through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 5-10 parts of Polygonum cuspidatum root powder, 40-60 parts of n-butyl ether and 10-20 parts of water by weight, and extract at a pressure of 5-10 MPa and a temperature of 35-45°C; then centrifuge at 4000-6000r / min for 10-20min, and filter to obtain filtrate A; add 0.2-0.4 parts of diatomaceous earth thereto, continue stirring at a temperature of 30-40°C for 2-3h, and concentrate the filtrate obtained by filtration at a temperature of 50-60°C for 5-10min to obtain Polygonum cuspidatum root extract.

[0014] Furthermore, the preparation process of the Polygonum cuspidatum root extract is optimized as follows: the washed and dried Polygonum cuspidatum root is sliced, crushed, and passed through a 10-20 mesh sieve to obtain the Polygonum cuspidatum root powder; 8 parts of Polygonum cuspidatum root powder, 55 parts of n-butyl ether and 16 parts of water are taken by weight, and extracted at a pressure of 7 MPa and a temperature of 40°C; then centrifuged at 5000 r / min for 15 minutes, and filtrated to obtain filtrate A; 0.3 parts of diatomaceous earth is added thereto, and stirring is continued at a temperature of 35°C for 2.5 hours, and the filtrate obtained by filtration is concentrated at a temperature of 55°C for 8 minutes to obtain the Polygonum cuspidatum root extract.

[0015] Furthermore, the preparation process of the Mongolian Astragalus extract described in step S3 is as follows: the washed and dried Mongolian Astragalus is crushed and passed through a 10-30 mesh sieve to obtain Mongolian Astragalus powder; 5-10 parts of Mongolian Astragalus powder and 50-100 parts of ethanol are taken by weight, extracted at a temperature of 80-100°C for 3-4h, filtered, and centrifuged at 3000-5000r / min to obtain the Mongolian Astragalus extract.

[0016] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. The present invention uses Cynomorium songaricum extract, Clove flower extract, Smilax glabra root extract, Sophora flavescens root extract, Phellodendron chinense bark extract, Polygonum cuspidatum root extract, Cistanche deserticola extract, Epimedium brevicornum extract, and Astragalus membranaceus extract as main compound ingredients; it combines the effects of Cynomorium songaricum, Clove flower, Epimedium brevicornum, Cistanche deserticola and Astragalus membranaceus on invigorating essence, strengthening yang and nourishing tendons; it combines the effects of Smilax glabra root, Sophora flavescens root, Phellodendron chinense bark and Polygonum cuspidatum root on clearing away heat and dampness, reducing inflammation and sterilizing; thereby making the obtained antibacterial gel have good antibacterial and anti-inflammatory and kidney-tonifying and yang-strengthening effects, and is more suitable for men's vulva.

[0017] 2. During the preparation of Cynomorium songaricum extract, the extraction efficiency and the content of effective ingredients are further improved through the synergistic effect of potassium sulfate aqueous solution and neutral protease.

[0018] 3. In the preparation process of lilac flower extract, through the joint action of potassium citrate, chitosan, vinyl triethoxysilane, sodium alginate, etc., the modified nano-silica can better protect the nutrients of lilac flowers, improve dispersion, and improve enzymatic efficiency, extraction efficiency and extraction effect.

[0019] 4. During the preparation of Polygonum cuspidatum root extraction, a small amount of diatomaceous earth is added to achieve the enrichment of active chemical substances in Polygonum cuspidatum root during the extraction process, thereby improving the extraction rate. Diatomaceous earth is insoluble in water or alcohol and can be removed by simple filtration. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Example 1 This embodiment provides a method for preparing an antibacterial gel, comprising the following steps: S1: Dry, clean, dry and crush Cynomorium songaricum, and then pass it through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; take 10g of Cynomorium songaricum powder, add 80g of distilled water thereto, and stir and mix at a rate of 500r / min for 0.5h; then add 0.1g of 0.1% potassium sulfate aqueous solution by mass, stir at a rate of 600r / min for 20min, adjust the pH to 7.3-7.5, centrifuge at a rate of 5000r / min for 25min, and take supernatant A; take 15g of supernatant and 0.2g of neutral protease, and perform enzymolysis reaction at a temperature of 45℃ for 1h; after the enzymolysis is completed, take supernatant B after static precipitation; centrifuge supernatant B at a rate of 5000r / min for 10min, and take supernatant C to obtain Cynomorium songaricum extract. 100 g of Cynomorium songaricum extract, 60 g of Sophora flavescens root extract and 50 g of Phellodendron chinense bark extract were mixed, and stirred at 800 r / min for 30 min at 40° C. to obtain a mixed solution A; the central protease, 50 U / mg, was from Hunan Yunbang Biotechnology Co., Ltd.

[0022] S2: 3 g of nano-silicon dioxide, 4 g of potassium citrate, 2 g of chitosan, 0.5 g of sodium alginate, 0.1 g of vinyl triethoxysilane, 10 g of n-butanol and 50 g of distilled water were mixed and stirred at 50 ° C for 1 h at a rate of 500 r / min to obtain a modifier; lilac flowers were frozen at -30 ° C and ground into lilac flower powder; 5 g of lilac flower powder and 30 g of the modifier were stirred and mixed at a temperature of 40 ° C at a rate of 500 r / min for 25 min , then add 0.2g cellulase and perform enzymolysis at 35°C for 1h; centrifuge at 4000r / min for 25min and take the supernatant to obtain lilac extract; mix 75g lilac extract, 100g Smilax glabra root extract and 130g Cistanche extract, stir and mix at 60°C at a speed of 600r / min for 40min to obtain mixed solution B; the cellulase, 50U / mg, is from Jiangsu Aofu Biotechnology Co., Ltd.

[0023] S3: Slice and crush the washed and dried Polygonum cuspidatum root, and pass it through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 5g of Polygonum cuspidatum root powder, 40g of n-butyl ether and 10g of water, extract at a pressure of 5MPa and a temperature of 35°C; then centrifuge at 4000r / min for 10min, filter to obtain filtrate A; add 0.2g of diatomaceous earth thereto, continue stirring at a temperature of 30°C for 2h, concentrate the filtrate obtained by filtration at a temperature of 50°C for 5min, and obtain Polygonum cuspidatum root extract; crush the washed and dried Mongolian Astragalus membranaceus, and pass it through a 10-30 mesh sieve to obtain Mongolian Astragalus membranaceus powder; take 5g of Mongolian Astragalus membranaceus Astragalus powder and 50g ethanol are extracted at a temperature of 80°C for 3h, filtered, and centrifuged at 3000r / min to obtain the upper layer liquid to obtain Mongolian Astragalus extract; 180g of Polygonum cuspidatum root extract, 110g of Epimedium extract and 90g of Mongolian Astragalus extract are mixed, and stirred at a speed of 500r / min for 20min at a temperature of 75°C; then, the mixed solution A obtained in step S1, the mixed solution B obtained in step S2, 10g of xanthan gum, and 0.2g of polyhexamethylene biguanide hydrochloride are added, and stirring is continued at a speed of 800r / min for 2h to obtain the antibacterial gel.

[0024] Example 2 This embodiment provides a method for preparing an antibacterial gel, comprising the following steps: S1: Dry, clean, dry and crush Cynomorium songaricum, and then pass it through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; take 20g of Cynomorium songaricum powder, add 120g of distilled water thereto, and stir and mix at a rate of 400r / min for 1h; then add 0.3g of 0.2% potassium sulfate aqueous solution, stir at a rate of 400r / min for 40min, adjust the pH to 7.9-8.1, centrifuge at a rate of 4000r / min for 35min, and take supernatant A; take 25g of supernatant and 0.5g of neutral protease, and perform enzymolysis reaction at a temperature of 55℃ for 2h; after the enzymolysis is completed, take supernatant B after static precipitation; centrifuge supernatant B at a rate of 4500 / min for 20min, and take supernatant C to obtain Cynomorium songaricum extract. 120 g of Cynomorium songaricum extract, 80 g of Sophora flavescens root extract and 70 g of Phellodendron chinense bark extract were mixed, and stirred at 60°C at a speed of 500 r / min for 50 min to obtain a mixed solution A; the central protease, 50 U / mg, was from Hunan Yunbang Biotechnology Co., Ltd.

[0025] S2: 5 g of nano-silicon dioxide, 7 g of potassium citrate, 5 g of chitosan, 1.5 g of sodium alginate, 0.3 g of vinyl triethoxysilane, 30 g of n-butanol and 100 g of distilled water were mixed, and stirred at 60 ° C at a rate of 500 r / min for 3 h to obtain a modifier; lilac flowers were frozen at -25 ° C and ground into lilac flower powder; 10 g of lilac flower powder and 60 g of the modifier were stirred and mixed at 50 ° C at a rate of 600 r / min for 35 min n, then adding 0.4g cellulase, enzymolysis at 45°C for 2h; centrifuging at 5000r / min for 35min, taking the supernatant to obtain lilac extract; mixing 95g lilac extract, 150g Smilax glabra root extract and 200g Cistanche extract, stirring and mixing at 80°C at a speed of 500r / min for 60min to obtain a mixed solution B; the cellulase, 50U / mg, Jiangsu Aofu Biotechnology Co., Ltd.

[0026] S3: Slice and crush the washed and dried Polygonum cuspidatum root, and pass it through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 10g Polygonum cuspidatum root powder, 60g n-butyl ether and 20g water, extract at a pressure of 10MPa and a temperature of 45°C; then centrifuge at 6000r / min for 20min, filter to obtain filtrate A; add 0.4g diatomaceous earth thereto, stir at a temperature of 40°C and a speed of 600r / min for 3h, concentrate the filtrate obtained by filtration at a temperature of 60°C for 10min, and obtain Polygonum cuspidatum root extract; The washed and dried Mongolian Astragalus was crushed and passed through a 10-30 mesh sieve to obtain Mongolian Astragalus powder; 10 g of Mongolian Astragalus powder and 100 g of ethanol were extracted at a temperature of 100° C. for 4 h, filtered, and centrifuged at 5000 r / min to obtain the upper layer liquid to obtain the Mongolian Astragalus extract; 250 g of Polygonum cuspidatum root extract, 160 g of Epimedium extract and 130 g of Astragalus membranaceus extract were mixed, and the mixture was stirred at 95° C. and a speed of 500 r / min for 30 min; then, the mixed solution A obtained in step S1, the mixed solution B obtained in step S2, 30 g of xanthan gum, and 0.6 g of polyhexamethylene biguanide hydrochloride were added, and the mixture was stirred at a speed of 600 r / min for 4 h to obtain the antibacterial gel.

[0027] Example 3 This embodiment provides a method for preparing an antibacterial gel, comprising the following steps: S1: Dry, clean, dry and crush Cynomorium songaricum, and then pass it through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; take 15g of Cynomorium songaricum powder, add 100g of distilled water thereto, and stir and mix at a rate of 500r / min for 0.8h; then add 0.25g of 0.15% potassium sulfate aqueous solution, stir at a rate of 400r / min for 35min, adjust the pH to 7.6-7.8, centrifuge at a rate of 5000r / min for 30min, and take supernatant A; take 20g of supernatant and 0.35g of neutral protease, and perform enzymolysis reaction at a temperature of 50℃ for 1.5h; after the enzymolysis is completed, take supernatant B after static precipitation; centrifuge supernatant B at a rate of 4500r / min for 15min, and take supernatant C to obtain Cynomorium songaricum extract. 110 g of Cynomorium songaricum extract, 70 g of Sophora flavescens root extract and 60 g of Phellodendron chinense bark extract were mixed, and stirred at 50°C and 500 r / min for 45 min to obtain a mixed solution A; the central protease, 50 U / mg, was from Hunan Yunbang Biotechnology Co., Ltd.

[0028] S2: 4 g of nano-silicon dioxide, 6 g of potassium citrate, 3 g of chitosan, 1 g of sodium alginate, 0.2 g of vinyl triethoxysilane, 25 g of n-butanol and 80 g of distilled water were mixed, and stirred at 55 ° C at a rate of 400 r / min for 1.5 h to obtain a modifier; lilac flowers were frozen at a temperature of -25 ° C and ground into lilac flower powder; 8 g of lilac flower powder and 45 g of the modifier were stirred and mixed at a temperature of 45 ° C at a rate of 400 r / min for 30 min. , then add 0.35g of cellulase and perform enzymolysis at 40°C for 1.5h; after centrifugation at 7000r / min for 30min, take the supernatant to obtain lilac flower extract; mix 80g of lilac flower extract, 120g of Smilax glabra root extract and 160g of Cistanche deserticola extract, stir and mix at 75°C at a speed of 500r / min for 45min to obtain mixed solution B; the cellulase, 50U / mg, is from Jiangsu Aofu Biotechnology Co., Ltd.

[0029] S3: Slice and crush the washed and dried Polygonum cuspidatum root, and pass it through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 8g of Polygonum cuspidatum root powder, 50g of n-butyl ether and 16g of water, extract at a pressure of 7MPa and a temperature of 40°C; then centrifuge at 5000r / min for 15min, filter to obtain filtrate A; add 0.3g of diatomaceous earth thereto, continue stirring at a temperature of 35°C and a speed of 500r / min for 2.5h, concentrate the filtrate obtained by filtration at a temperature of 55°C for 8min, and obtain Polygonum cuspidatum root extract; The washed and dried Mongolian Astragalus was crushed and passed through a 10-30 mesh sieve to obtain Mongolian Astragalus powder; 7g of Mongolian Astragalus powder and 80g of ethanol were extracted at a temperature of 95°C for 3.5h, filtered, and centrifuged at 4500r / min to obtain the upper layer liquid to obtain the Mongolian Astragalus extract; 200 g of Polygonum cuspidatum root extract, 135 g of Epimedium extract and 110 g of Astragalus membranaceus extract were mixed, and the mixture was stirred at 90°C at a rate of 500 r / min for 25 min; then, the mixed solution A obtained in step S1, the mixed solution B obtained in step S2, 16 g of xanthan gum, and 0.5 g of polyhexamethylene biguanide hydrochloride were added, and the mixture was stirred at a rate of 800 r / min for 3.5 h to obtain the antibacterial gel.

[0030] In the above examples, the Smilax glabra root extract, Sophora flavescens root extract, Phellodendron chinense bark extract, Cistanche deserticola extract, and Epimedium brevicornum extract were all purchased from Shaanxi Snort Biotechnology.

[0031] Comparative Example 1 The difference between this comparative example and Example 1 is that the Cynomorium songaricum extract in step S1 adopts the product of Snort, and the other steps are the same.

[0032] Comparative Example 2 The difference between this comparative example and Example 1 is that the clove (flower) extract in step S2 adopts the product of Snort, and the other steps are the same.

[0033] Comparative Example 3 The difference between this comparative example and Example 1 is that the root extract of Polygonum cuspidatum in step S3 adopts Shaanxi Kehong Health Products, and the other steps are the same.

[0034] Comparative Example 4 The difference between this comparative example and Example 1 is that the Mongolian Astragalus extract in step S3 adopts Xi'an Plait Biological Products, and the other steps are the same.

[0035] Comparative Example 5 The difference between this comparative example and Example 1 is that the Sophora flavescens root extract and the Phellodendron chinense bark extract are not added in step S1, and the other steps are the same.

[0036] Comparative Example 6 The difference between this comparative example and Example 1 is that no modifier is added in step S2, and the other steps are the same.

[0037] Performance Test: The antibacterial gel in the above examples and comparative examples was subjected to antibacterial experiments. The test bacteria included: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. The specific experimental method was as follows: 5 mL of the antibacterial gel in the examples and comparative examples were respectively taken and injected into sterile plates; the pre-prepared bacterial slices of each test bacterial species were immersed in the antibacterial gel for 3 minutes. The bacterial slices were respectively transferred into test tubes, sterile physiological saline was added, and fully shaken; after shaking and mixing, 1.0 ml was drawn and coated on a plate for culture. After the colonies grew out, they were counted and the sterilization rate was calculated. The results are shown in the following table.

[0038] Table 1 Performance test results

[0039] From the above performance test results, it can be seen that the antibacterial rates of Examples 1-3 are all above 98.8%, and the antibacterial effects are obvious; in particular, the comprehensive performance of Example 3 is the most outstanding. However, since Comparative Examples 1-6 do not adopt the necessary technical solutions, their corresponding performance tests are significantly worse than those of the Examples. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0040] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An antibacterial gel for male vulva, characterized in that: The invention comprises the following components in parts by weight: 0.2-0.6 parts of polyhexamethylene biguanide hydrochloride, 10-30 parts of xanthan gum, 90-130 parts of cynomorium songaricum extract, 75-95 parts of lilac flower extract, 100-150 parts of smilax glabra root extract, 50-90 parts of sophora flavescens root extract, 40-80 parts of phellodendron chinense bark extract, 180-250 parts of polygonum cuspidatum root extract, 130-200 parts of cistanche extract, 110-160 parts of epimedium extract and 90-130 parts of astragalus membranaceus extract.

2. The antibacterial gel for male vulva according to claim 1, characterized in that: In parts by weight, the polyhexamethylene biguanide hydrochloride is 0.3-0.4 parts, xanthan gum is 15-25 parts, Cynomorium songaricum extract is 100-120 parts, clove flower extract is 80-90 parts, Smilax glabra root extract is 110-130 parts, Sophora flavescens root extract is 60-80 parts, Phellodendron chinense bark extract is 50-70 parts, Polygonum cuspidatum root extract is 200-230 parts, Cistanche deserticola extract is 150-180 parts, Epimedium brevicornum extract is 120-140 parts, and Astragalus membranaceus extract is 100-120 parts.

3. A method for preparing an antibacterial gel for male vulva, characterized in that: The steps include: S1: Mix 100-120 parts of Cynomorium songaricum extract, 60-80 parts of Sophora flavescens root extract and 50-70 parts of Phellodendron chinense bark extract by weight, and stir and mix at a temperature of 40-60° C. for 30-50 minutes to obtain a mixed solution A; S2: Mix 75-95 parts of clove flower extract, 100-150 parts of Smilax glabra root extract and 130-200 parts of Cistanche deserticola extract by weight, and stir and mix at a temperature of 60-80° C. for 40-60 minutes to obtain a mixed solution B; S3: Mix 180-250 parts of Polygonum cuspidatum root extract, 110-160 parts of Epimedium extract and 90-130 parts of Astragalus membranaceus extract by weight, and stir the mixture at a temperature of 75-95°C for 20-30 minutes; then add the mixed solution A obtained in step S1, the mixed solution B obtained in step S2, 10-30 parts of xanthan gum, and 0.2-0.6 parts of polyhexamethylene biguanide hydrochloride, and continue stirring for 2-4 hours to obtain the antibacterial gel.

4. The method for preparing an antibacterial gel for male vulva according to claim 3, characterized in that: The preparation process of the Cynomorium songaricum extract in step S1 is as follows: air-dry, wash, dry, and crush the Cynomorium songaricum, and then pass it through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; take 10-20 parts of Cynomorium songaricum powder by weight, add 80-120 parts of distilled water thereto, and stir and mix for 0.5-1h; then add 0.1-0.3 parts of a 0.1-0.2% potassium sulfate aqueous solution, stir for 20-40min, adjust the pH to 7.3-8.1, and centrifuge for 25-35min to obtain supernatant A; take 15-25 parts of the supernatant and 0.2-0.5 parts of neutral protease by weight, and perform enzymolysis reaction at a temperature of 45-55°C for 1-2h; after the enzymolysis is completed, take supernatant B after static precipitation; centrifuge supernatant B for 10-20min, and then take supernatant C to obtain Cynomorium songaricum extract.

5. The method for preparing an antibacterial gel for male vulva according to claim 4, characterized in that: The preparation process of Cynomorium songaricum extract is optimized as follows: Cynomorium songaricum is air-dried, washed, dried, and crushed, and then passed through a 5-10 mesh sieve to obtain Cynomorium songaricum powder; 15 parts of Cynomorium songaricum powder are taken by weight, 100 parts of distilled water are added thereto, and stirred for 0.8 hours; 0.25 parts of a 0.15% potassium sulfate aqueous solution are added, and stirred for 35 minutes, the pH is adjusted to 7.6-7.8, and the supernatant A is obtained after centrifugation for 30 minutes; 20 parts of supernatant and 0.35 parts of neutral protease were taken by weight, and enzymatic hydrolysis was carried out at a temperature of 50°C for 1.5 hours; after the enzymatic hydrolysis was completed, supernatant B was taken after static precipitation; supernatant B was centrifuged for 15 minutes, and supernatant C was taken to obtain Cynomorium songaricum extract.

6. The method for preparing the antibacterial gel for male vulva according to claim 3, characterized in that: The preparation process of the lilac extract in step S2 is as follows: by weight, 3-5 parts of nano-silicon dioxide, 4-7 parts of potassium citrate, 2-5 parts of chitosan, 0.5-1.5 parts of sodium alginate, 0.1-0.3 parts of vinyl triethoxysilane, 10-30 parts of n-butanol and 50-100 parts of distilled water are mixed, and stirred at a temperature of 50-60° C. for 1-3 hours to obtain a modifier; the lilac flowers are frozen and ground into lilac powder; 5-10 parts of lilac powder and 30-60 parts of the modifier are stirred and mixed at a temperature of 40-50° C. for 25-35 minutes, and then 0.2-0.4 parts of cellulase are added, and enzymolysis is performed at a temperature of 35-45° C. for 1-2 hours; and the supernatant is taken after centrifugal treatment at 4000-5000 r / min for 25-35 minutes to obtain the lilac extract.

7. The method for preparing an antibacterial gel for male vulva according to claim 6, characterized in that: The preparation process of lilac flower extract is optimized as follows: by weight, 4 parts of nano-silicon dioxide, 6 parts of potassium citrate, 3 parts of chitosan, 1 part of sodium alginate, 0.2 parts of vinyltriethoxysilane, 25 parts of n-butanol and 80 parts of distilled water are mixed, and stirred at a temperature of 55°C for 1.5 hours to obtain a modifier; the lilac flowers are frozen and ground into lilac flower powder; 8 parts of lilac flower powder and 45 parts of the modifier are stirred and mixed at a temperature of 45°C for 30 minutes, and then 0.35 parts of cellulase are added, and enzymolysis is carried out at a temperature of 40°C for 1.5 hours; after centrifugation at 7000r / min for 30 minutes, the supernatant is taken to obtain the lilac flower extract.

8. The method for preparing the antibacterial gel for male vulva according to claim 3, characterized in that: The preparation process of the Polygonum cuspidatum root extract in step S3 is as follows: slice, crush and pass the washed and dried Polygonum cuspidatum root through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 5-10 parts of Polygonum cuspidatum root powder, 40-60 parts of n-butyl ether and 10-20 parts of water by weight, and extract at a pressure of 5-10 MPa and a temperature of 35-45°C; then centrifuge at 4000-6000 r / min for 10-20 minutes, and filter to obtain filtrate A; add 0.2-0.4 parts of diatomaceous earth thereto, continue stirring at a temperature of 30-40°C for 2-3 hours, and concentrate the filtrate obtained by filtration at a temperature of 50-60°C for 5-10 minutes to obtain Polygonum cuspidatum root extract.

9. The method for preparing an antibacterial gel for male vulva according to claim 8, characterized in that: The preparation process of Polygonum cuspidatum root extract is optimized as follows: slice, crush and pass the washed and dried Polygonum cuspidatum root through a 10-20 mesh sieve to obtain Polygonum cuspidatum root powder; take 8 parts of Polygonum cuspidatum root powder, 55 parts of n-butyl ether and 16 parts of water by weight, and extract at a pressure of 7 MPa and a temperature of 40°C; then centrifuge at 5000 r / min for 15 minutes, and filter to obtain filtrate A; add 0.3 parts of diatomaceous earth thereto, continue stirring at a temperature of 35°C for 2.5 hours, and concentrate the filtrate obtained by filtration at a temperature of 55°C for 8 minutes to obtain Polygonum cuspidatum root extract.

10. The method for preparing the antibacterial gel for male vulva according to claim 3, characterized in that: The preparation process of the Mongolian Astragalus extract in step S3 is as follows: the washed and dried Mongolian Astragalus is crushed and passed through a 10-30 mesh sieve to obtain Mongolian Astragalus powder; 5-10 parts of Mongolian Astragalus powder and 50-100 parts of ethanol are taken by weight, extracted at a temperature of 80-100°C for 3-4h, filtered, and centrifuged at 3000-5000r / min to obtain the Mongolian Astragalus extract.

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