3D magnetic suspension cell culture device

Abstract

The invention provides a 3D magnetic suspension cell culture device which comprises a magnetic steel assembly, the magnetic steel assembly is cylindrical and comprises two sets of first magnetic steel, two sets of second magnetic steel and three sets of third magnetic steel, the two sets of first magnetic steel are oppositely arranged, the directions of magnetic induction lines in the two sets of first magnetic steel are the same, and the magnetic induction lines in the two sets of first magnetic steel are opposite to each other. The extension lines of the magnetic induction lines in the two groups of first magnetic steel pass through the axis of the magnetic steel assembly, the two groups of third magnetic steel are oppositely arranged, the directions of the magnetic induction lines in the two groups of third magnetic steel are the same, and the directions of the magnetic induction lines in the third magnetic steel are opposite to the directions of the magnetic induction lines in the first magnetic steel; the number of the second magnetic steel is four, the second magnetic steel is arranged between the first magnetic steel and the third magnetic steel, the directions of magnetic induction lines in the two sets of second magnetic steel which are oppositely arranged are the same, and the magnetic induction lines in the two sets of second magnetic steel on the two circumferential sides of one set of first magnetic steel likewise point to the radial inner side or the radial outer side.

Description

Technical Field

[0001] The present application belongs to the field of cell culture technology, and in particular relates to a 3D magnetic suspension cell culture device. Background Art

[0002] In the body, cells are in a 3D environment and have extensive and complex interactions with the surrounding nuclei and extracellular matrix. 2D cell culture refers to the culture of cells in a 2D state at the bottom of a culture dish under the action of gravity in a culture medium. 2D cell culture lacks the interaction between cells and the complexity of the extracellular matrix. 2D culture cannot simulate the environment in the body, resulting in differences in cell behavior and function from that in the body.

[0003] The most widely used 3D culture at present is hydrogel 3D culture. The structure of hydrogel limits the effective diffusion of oxygen, nutrients and metabolic waste, resulting in an uneven living environment for cells, nutritional deficiency or accumulation of metabolic products in some cells. Summary of the invention

[0004] The present application aims to propose a 3D magnetic levitation cell culture device, so that the cell culture environment can simulate the environment in vivo as much as possible.

[0005] The embodiment of the present application provides a 3D magnetic suspension cell culture device, including a magnetic steel component, the magnetic steel component is cylindrical, and the magnetic steel component includes: a first magnetic steel, a second magnetic steel and a third magnetic steel, the first magnetic steel, the second magnetic steel and the third magnetic steel form a closed ring,

[0006] in,

[0007] At least two groups of the first magnetic steel are provided, and the two groups of the first magnetic steel are arranged opposite to each other, and the directions of the magnetic flux lines inside the two groups of the first magnetic steel are the same, and the extension lines of the magnetic flux lines inside the two groups of the first magnetic steel pass through the axis of the magnetic steel assembly.

[0008] The third magnetic steels are provided with at least two groups, the two groups of the third magnetic steels are arranged opposite to each other, the directions of the magnetic flux lines inside the two groups of the third magnetic steels are the same, and the directions of the magnetic flux lines inside the third magnetic steels are opposite to the directions of the magnetic flux lines inside the first magnetic steels.

[0009] At least four groups of the second magnetic steels are provided. The second magnetic steels are provided between the first magnetic steels and the third magnetic steels. The magnetic flux lines inside the two oppositely arranged groups of the second magnetic steels are in the same direction. The magnetic flux lines inside one group of the first magnetic steels and the two groups of the second magnetic steels on both sides of the group of the first magnetic steels in the circumferential direction are directed in the same direction radially inward or radially outward.

[0010] Thus, a magnetic field is formed in the hollow portion of the magnetic steel assembly, which is directed from one group of the first magnetic steels among the two groups of the first magnetic steels to the other group of the first magnetic steels.

[0011] In at least one possible implementation, an angle between a direction of an internal magnetic flux line of the second magnetic steel and a direction of an internal magnetic flux line of the first magnetic steel is 45 degrees to 90 degrees.

[0012] In at least one possible implementation, the direction of the magnetic flux lines inside the second magnetic steel extends along the radial direction of the magnetic steel assembly.

[0013] In at least one possible implementation, the angle between the direction of the internal magnetic flux lines of the second magnetic steel and the direction of the internal magnetic flux lines of the third magnetic steel is 90 degrees.

[0014] The directions of the internal magnetic flux lines of the two groups of the second magnetic steel on both sides of the circumference of the first magnetic steel with the directions of the internal magnetic flux lines pointing radially inward are arranged opposite to each other.

[0015] The directions of the internal magnetic flux lines of two groups of second magnetic steels on both sides of the circumferential direction of the first magnetic steel whose internal magnetic flux lines are directed radially outward are arranged in opposite directions.

[0016] In at least one possible implementation manner, a magnetic energy product of the third magnetic steel is greater than a magnetic energy product of the first magnetic steel.

[0017] In at least one possible implementation manner, a magnetic energy product of the third magnetic steel is greater than a magnetic energy product of the second magnetic steel.

[0018] In at least one possible implementation manner, the magnetic energy product of the first magnetic steel and the magnetic energy product of the second magnetic steel are both 35 mega-Gauss-Oersted, and the magnetic energy product of the third magnetic steel is 52 mega-Gauss-Oersted.

[0019] In at least one possible implementation manner, the circumferential dimensions of the first magnetic steel, the second magnetic steel, and the third magnetic steel are the same.

[0020] In at least one possible embodiment, the 3D magnetic suspension cell culture device further includes a shell, which surrounds the magnetic steel assembly.

[0021] In at least one possible implementation, the housing is made of a magnetic shielding material.

[0022] By adopting the above technical solution, a magnetic field with a large central magnetic field intensity and a small edge magnetic field intensity is generated in the working area, so that the magnetically labeled cells to be cultured can be suspended in the cell culture medium, simulating the 3D stereoscopic environment in the organism for the cells, thereby enabling high-throughput rapid culture and replication of cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A partial structural schematic diagram of a 3D magnetic suspension cell culture device according to the first embodiment of the present application is shown.

[0024] Figure 2 A coordinate diagram showing the variation of magnetic field intensity in the X direction of the working area of ​​the 3D magnetic suspension cell culture device according to the first embodiment of the present application is shown.

[0025] Figure 3 A coordinate diagram showing the change in magnetic field intensity in the Y direction of the working area of ​​the 3D magnetic suspension cell culture device according to the first embodiment of the present application is shown.

[0026] Figure 4 A coordinate diagram showing the variation of magnetic field intensity in the axial direction of the working area of ​​the 3D magnetic suspension cell culture device according to the first embodiment of the present application is shown.

[0027] Figure 5 A partial structural schematic diagram of a 3D magnetic suspension cell culture device according to a second embodiment of the present application is shown.

[0028] Figure 6 A coordinate diagram showing the variation of magnetic field intensity in the X direction of the working area of ​​the 3D magnetic suspension cell culture device according to the second embodiment of the present application is shown.

[0029] Figure 7 A coordinate diagram showing the change in magnetic field intensity in the Y direction of the working area of ​​the 3D magnetic suspension cell culture device according to the second embodiment of the present application is shown.

[0030] Figure 8 A coordinate diagram of the change in magnetic field intensity in a direction with an angle of 45 degrees with the X direction and the Y direction in a working area of ​​a 3D magnetic suspension cell culture device according to the second embodiment of the present application is shown.

[0031] Description of Reference Numerals

[0032] 1 Magnetic steel assembly 11 First magnetic steel 12 Second magnetic steel 13 Third magnetic steel

[0033] 2 Housing

[0034] C Circumferential DETAILED DESCRIPTION

[0035] In order to more clearly explain the above-mentioned purposes, features and advantages of the present application, the specific implementation methods of the present application are described in detail in this section in conjunction with the accompanying drawings. In addition to the various implementation methods described in this section, the present application can also be implemented in other different ways. Without violating the spirit of the present application, those skilled in the art can make corresponding improvements, deformations and substitutions, so the present application is not limited by the specific embodiments disclosed in this section. The scope of protection of the present application shall be subject to the claims.

[0036] (First Embodiment)

[0037] like Figures 1 to 4 As shown, the first embodiment of the present application provides a 3D magnetic suspension cell culture device, which includes a magnetic steel component 1 and a shell 2. The magnetic steel component 1 can be a hollow cylinder, and the shell 2 can surround the magnetic steel component 1.

[0038] The magnetic steel assembly 1 comprises a first magnetic steel 11 , a second magnetic steel 12 and a third magnetic steel 13 , that is, when viewed along the axial direction of the magnetic steel assembly 1 , the first magnetic steel 11 , the second magnetic steel 12 and the third magnetic steel 13 form a closed circular ring.

[0039] At least two or two groups of first magnetic steels 11 may be provided. The two or two groups of first magnetic steels 11 may be arranged opposite to each other, that is, when viewed along the axial direction of the magnetic steel assembly 1, the centers of the two or two groups of first magnetic steels 11 form an angle of 180 degrees. The directions of the magnetic flux lines inside the two or two groups of first magnetic steels 11 are the same. For example, Figure 1 In the figure, the directions of the magnetic flux lines inside the two first magnetic steels 11 are both downward. The extension lines of the magnetic flux lines inside the two or two groups of first magnetic steels 11 pass through the axis of the magnetic steel assembly 1, and the direction of the magnetic field formed in the hollow part of the magnetic steel assembly 1 (i.e., the working area W) can be roughly from one of the first magnetic steels 11 to the other first magnetic steel 11, that is, the magnetic field in the working area W has two poles.

[0040] At least two or two groups of third magnetic steels 13 may be provided. The two or two groups of third magnetic steels 13 may be arranged opposite to each other, that is, when viewed along the axial direction of the magnetic steel assembly 1, the centers of the two or two groups of third magnetic steels 13 form an angle of 180 degrees. The directions of the magnetic flux lines inside the two or two groups of third magnetic steels 13 are the same, and the directions of the magnetic flux lines inside the third magnetic steel 13 are opposite to the directions of the magnetic flux lines inside the first magnetic steel. For example, Figure 1 In the figure, the directions of the magnetic flux lines inside the two third magnetic steels 13 are both upward.

[0041] In the circumferential direction C of the magnetic steel assembly 1 , the center of the third magnetic steel 13 and the center of the first magnetic steel 11 may form an angle of 90 degrees.

[0042] At least four or four groups of second magnetic steels 12 may be provided, and the second magnetic steels 12 may be provided between the first magnetic steels 11 and the third magnetic steels 13. In this embodiment, two first magnetic steels 11, four second magnetic steels 12 and two third magnetic steels 13 form a closed ring, and there is a second magnetic steel between each first magnetic steel 11 and the third magnetic steel 13.

[0043] The magnetic flux lines inside the two oppositely arranged groups of second magnetic steels 12 have the same direction, and the magnetic flux lines inside the two groups of second magnetic steels 12 on both sides of the first magnetic steel 11 and the first magnetic steel 11 sandwiched between them also point radially inward or radially outward.

[0044] It can be understood that each group of first magnetic steels 11 here may include one or more first magnetic steels. In one example, when a group of first magnetic steels 11 includes multiple first magnetic steels, the multiple first magnetic steels in a group of first magnetic steels 11 may be arranged side by side and close to each other, and the directions of the internal magnetic flux lines of the multiple first magnetic steels in a group of first magnetic steels 11 may be the same or approximately the same. For example, referring to Figure 1 The directions of the internal magnetic flux lines of a plurality of first magnetic steels in a group of first magnetic steels 11 may all be from top to bottom.

[0045] Likewise, each group of second magnetic steels may also include one or more second magnetic steels. Each group of third magnetic steels may also include one or more third magnetic steels.

[0046] It can be understood that the direction of the magnetic flux lines pointing radially inwards or radially outwards does not mean that the magnetic flux lines extend radially, and the direction of the magnetic flux lines may form a certain angle with the radial direction.

[0047] Furthermore, the angle between the direction of the internal magnetic flux lines of the second magnetic steel 12 and the direction of the internal magnetic flux lines of the first magnetic steel 11 may be 45 degrees to 90 degrees.

[0048] Furthermore, the magnetic flux lines inside the second magnetic steel 12 may extend radially along the magnetic steel assembly 1. The two second magnetic steels 12 on both sides of the first magnetic steel 11 may point radially inward or radially outward like the magnetic flux lines inside the first magnetic steel 11 sandwiched between them.

[0049] The circumferential dimensions of the first magnetic steel 11 , the second magnetic steel 12 , and the third magnetic steel 13 may be the same.

[0050] The direction of the magnetic flux lines of the magnetic field formed by the magnetic steel assembly 1 in the working area W can be directed from one first magnetic steel 11 to another first magnetic steel 11, that is, the magnetic field in the working area W forms a pair of N and S poles.

[0051] Figure 2 The X direction of the working area ( Figure 1The magnetic field strength at different positions on the left and right directions in the working area (in the horizontal direction). The horizontal axis represents the position in the X direction of the working area, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger at the center of the working area W, and the magnetic field is weaker at the edge of the working area W.

[0052] Figure 3 It shows the Y direction of the working area ( Figure 1 The magnetic field strength at different positions in the vertical direction (in the vertical direction). The horizontal axis represents the position in the Y direction of the working area, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger at the center of the working area W and weaker at the edge of the working area W.

[0053] Figure 4 The axial direction (perpendicular to the Figure 1 The horizontal axis represents the position in the axial direction of the working area, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger in the middle position in the axial direction of the working area W, and the magnetic field is weaker at the two ends of the axial direction of the working area W.

[0054] It can be understood that the X direction and the Y direction are both radial directions of the magnetic steel assembly 1, and the X direction and the Y direction are perpendicular to each other.

[0055] The working area W can be used to place a test tube so that the magnetic steel assembly 1 surrounds the test tube, which is filled with a cell culture solution and magnetically labeled cells to be cultured. The cells to be cultured can be suspended in the cell culture solution in the magnetic field, thereby simulating an environment similar to that in a living body, which is conducive to cell culture. The magnetic steel assembly 1 formed by setting the magnetic circuit in this way can have a stronger magnetic field at the center of the working area W away from the magnetic steel assembly 1, and a weaker magnetic field at the edge of the working area W close to the magnetic steel assembly 1. Since the magnetic field is weak at the edge of the working area W, it can avoid or reduce the concentration of magnetically labeled cells to be cultured on the wall or the bottom of the test tube under the action of the magnetic field, so that the cells to be cultured are suspended and agglomerated in the cell culture solution, simulating the 3D environment in the body, and can quickly culture and replicate cells with a large flux. If the cells to be cultured are concentrated on the wall or the bottom of the test tube, it is equivalent to the culture environment being a 2D plane space, and the cell culture effect is not good.

[0056] Further, the magnetic energy product of the first magnetic steel 11 and the magnetic energy product of the second magnetic steel 12 are different from the magnetic energy product of the third magnetic steel 13, and the magnetic energy product of the third magnetic steel 13 can be greater than the magnetic energy product of the first magnetic steel 11, and the magnetic energy product of the third magnetic steel 13 can be greater than the magnetic energy product of the second magnetic steel 12. The magnetic energy product of the first magnetic steel 11 and the second magnetic steel 12 can be the same. For example, the magnetic energy product of the first magnetic steel 11 and the magnetic energy product of the second magnetic steel 12 can both be 35 mega-Gauss Oersted (MGOe), that is, the model of the first magnetic steel 11 and the second magnetic steel 12 is N35, and the magnetic energy product of the third magnetic steel 13 can be 52 mega-Gauss Oersted (MGOe), that is, the model of the third magnetic steel 13 is N52. In this way, the magnetic field strength at the middle position of the working area W can be further improved.

[0057] The housing 2 can be made of magnetic shielding materials such as aluminum alloy and stainless steel. The housing 2 can shield the magnetic field, making the magnetic field in the non-working area smaller.

[0058] (Second Embodiment)

[0059] like Figures 5 to 8 As shown, the second embodiment of the present application provides a 3D magnetic suspension cell culture device, which includes a magnetic steel component 1 and a shell 2. The magnetic steel component 1 can be a hollow cylinder, and the shell 2 can surround the magnetic steel component 1.

[0060] The difference between the 3D magnetic suspension cell culture device of the second embodiment and the 3D magnetic suspension cell culture device of the first embodiment lies in the magnetic field direction of the second magnetic steel 12 of the magnetic steel assembly 1 .

[0061] At least four or four groups of second magnetic steels 12 may be provided, and the second magnetic steels 12 may be provided between the first magnetic steels 11 and the third magnetic steels 13. In this embodiment, two first magnetic steels 11, four second magnetic steels 12 and two third magnetic steels 13 form a closed ring, and there is a second magnetic steel between each first magnetic steel 11 and the third magnetic steel 13. Adjacent magnetic steels may be tightly attached together by being wrapped by the housing 2.

[0062] The direction of the magnetic flux lines inside the second magnetic steel 12 can extend along the X direction of the magnetic steel assembly 1, and the directions of the magnetic flux lines inside the two oppositely arranged second magnetic steels 12 are the same. The directions of the magnetic flux lines inside the two second magnetic steels 12 on both sides of the circumference of the first magnetic steel 11 are opposite, and the directions of the magnetic flux lines inside the two second magnetic steels 12 on both sides of the circumference of the third magnetic steel 13 are opposite.

[0063] The magnetic flux lines inside the second magnetic steel 12 and the magnetic flux lines inside the first magnetic steel 11 may be at an angle of 90 degrees (including approximately 90 degrees). The magnetic flux lines inside the second magnetic steel 12 and the magnetic flux lines inside the third magnetic steel 13 may be at an angle of 90 degrees (including approximately 90 degrees).

[0064] The directions of the internal magnetic flux lines of the two second magnetic steels 12 on both sides of the circumferential direction of the first magnetic steel 11 with the direction of the internal magnetic flux lines pointing radially inward are arranged opposite to each other.

[0065] The internal magnetic flux lines of the two second magnetic steels 12 on both sides of the circumferential direction of the first magnetic steel 11 with the internal magnetic flux lines pointing radially outward are arranged in opposite directions.

[0066] Figure 6 The X direction of the working area ( Figure 5 The magnetic field strength at different positions on the left and right directions in the working area (in the horizontal direction). The horizontal axis represents the position in the X direction of the working area, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger at the center of the working area W, and the magnetic field is weaker at the edge of the working area W.

[0067] Figure 7 It shows the Y direction of the working area ( Figure 5 The magnetic field strength at different positions in the vertical direction (in the vertical direction). The horizontal axis represents the position in the Y direction of the working area, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger at the center of the working area W and weaker at the edge of the working area W.

[0068] Figure 8 The magnetic field strength at different positions in the axial direction of the working area is shown. The horizontal axis represents the position in the direction of the working area with an angle of 45 degrees to the X direction and the Y direction, and the vertical axis represents the magnetic field strength. It can be seen that the magnetic field is stronger at the middle position in the direction of the working area W with an angle of 45 degrees to the X direction and the Y direction, and the magnetic field is weaker at the two end positions in the direction of the working area W with an angle of 45 degrees to the X direction and the Y direction.

[0069] It should be understood that at least some aspects or features of the above-mentioned implementation modes, embodiments or examples may be appropriately combined.

[0070] It is understood that in the present application, when the number of parts or components is not particularly limited, the number may be one or more, and the multiple here means two or more. For the case where the number of parts or components shown in the drawings and / or described in the specification is a specific number such as two, three, four, etc., the specific number is usually exemplary and not restrictive, and it can be understood as multiple, that is, two or more, but this does not mean that the present application excludes the case of one.

[0071] In the present application, unless otherwise clearly stated or limited, the terms such as "install", "assemble", "assemble", "connect", "connect", "couple", "link", "abut", "connect", "interconnect", "intercommunication", "conduction", "fixation", "fastening" and the like should be understood in a broad sense, for example, they can be direct or indirect. For example, regarding connection, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly stated or limited. For example, regarding connectivity / conduction, it can be direct connectivity / conduction, or it can be indirect connectivity / conduction via an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0072] In the present application, unless otherwise clearly stated or limited, a component disposed on / installed on / located on / accommodated on / placed in, within, inside, etc. another component may be any of the following two situations: a part or most of the one component is located in the other component; and the one component is completely accommodated in the other component.

[0073] Although the present application is described in detail using the above-mentioned embodiments, it is obvious to those skilled in the art that the present application is not limited to the embodiments described in this specification. The present application can be modified and implemented as a modified embodiment without departing from the subject matter and scope of the present application as determined by the claims. Therefore, the description in this specification is for the purpose of illustration and does not have any restrictive meaning for the present application.

Claims

1. A 3D magnetic suspension cell culture device, characterized in that: The invention comprises a magnetic steel component (1), wherein the magnetic steel component (1) is cylindrical, and comprises: a first magnetic steel (11), a second magnetic steel (12) and a third magnetic steel (13), wherein the first magnetic steel (11), the second magnetic steel (12) and the third magnetic steel (13) form a closed circular ring. in, At least two groups of the first magnetic steel (11) are provided, the two groups of the first magnetic steel (11) are arranged opposite to each other, the directions of the magnetic flux lines inside the two groups of the first magnetic steel (11) are the same, and the extension lines of the magnetic flux lines inside the two groups of the first magnetic steel (11) pass through the axis of the magnetic steel assembly (1), At least two groups of the third magnetic steels (13) are provided, and the two groups of the third magnetic steels (13) are arranged opposite to each other. The directions of the magnetic flux lines inside the two groups of the third magnetic steels (13) are the same, and the directions of the magnetic flux lines inside the third magnetic steels (13) are opposite to the directions of the magnetic flux lines inside the first magnetic steel (11). At least four groups of the second magnetic steels (12) are provided. The second magnetic steels (12) are provided between the first magnetic steels (11) and the third magnetic steels (13). The directions of the magnetic flux lines inside the two groups of the second magnetic steels (12) arranged opposite to each other are the same. The magnetic flux lines inside one group of the first magnetic steels (11) and the two groups of the second magnetic steels (12) on both sides of the circumferential direction of the first magnetic steels (11) are the same as pointing radially inward or radially outward. Thus, a magnetic field is formed in the hollow portion of the magnetic steel assembly (1), with one group of the first magnetic steels (11) pointing to the other group of the first magnetic steels (11).

2. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The angle between the direction of the internal magnetic flux lines of the second magnetic steel (12) and the direction of the internal magnetic flux lines of the first magnetic steel (11) is 45 degrees to 90 degrees.

3. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The direction of the magnetic flux lines inside the second magnetic steel (12) extends along the radial direction of the magnetic steel assembly (1).

4. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The direction of the internal magnetic flux lines of the second magnetic steel (12) and the direction of the internal magnetic flux lines of the third magnetic steel (13) are at an angle of 90 degrees. The directions of the internal magnetic flux lines of two groups of the second magnetic steel (12) on both sides of the circumference of the first magnetic steel (11) with the directions of the internal magnetic flux lines pointing radially inward are arranged opposite to each other. The internal magnetic flux lines of two groups of second magnetic steels (12) on both sides of the circumferential direction of the first magnetic steel (11) whose internal magnetic flux lines are directed radially outward are arranged in opposite directions.

5. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The magnetic energy product of the third magnetic steel (13) is greater than the magnetic energy product of the first magnetic steel (11).

6. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The magnetic energy product of the third magnetic steel (13) is greater than the magnetic energy product of the second magnetic steel (12).

7. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The magnetic energy product of the first magnetic steel (11) and the magnetic energy product of the second magnetic steel (12) are both 35 mega-Gauss-Oersted, and the magnetic energy product of the third magnetic steel (13) is 52 mega-Gauss-Oersted.

8. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The circumferential dimensions of the first magnetic steel (11), the second magnetic steel (12) and the third magnetic steel (13) are the same.

9. The 3D magnetic suspension cell culture device according to claim 1, characterized in that: The 3D magnetic suspension cell culture device further comprises a shell (2), wherein the shell (2) surrounds the magnetic steel component (1).

10. The 3D magnetic suspension cell culture device according to claim 9, characterized in that: The housing (2) is made of magnetic shielding material.

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