Nanozyme-based detection method for pesticide residues in traditional Chinese medicine

Abstract

The present invention discloses a method for detecting pesticide residues in Chinese medicinal materials based on nanozymes, and relates to the technical field of pesticide detection. The method for detecting pesticide residues in Chinese medicinal materials based on nanozymes comprises the following steps: obtaining a residual interference degree value; obtaining an enzyme reaction effective value; obtaining an enzyme reaction interference value. The present invention obtains a residual interference degree value by obtaining absorbance data of a Chinese medicinal material solution and reference absorbance data, and judges whether to obtain an enzyme reaction effective value, and then judges whether to adjust the enzyme reaction based on the obtained enzyme reaction effective value, and finally obtains an enzyme reaction interference value based on the enzyme reaction effective value after the enzyme reaction adjustment and signal change data, and judges whether to obtain the pesticide residue concentration data of a preset detector, thereby achieving a more accurate effect of detecting pesticide residues in Chinese medicinal materials. The problem of obvious interference in the detection process of pesticide residues in Chinese medicinal materials in the prior art is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide detection, and in particular to a method for detecting pesticide residues in traditional Chinese medicine based on nanozymes. Background Art

[0002] Nanozymes are a new generation of enzyme mimics that have the unique properties of nanomaterials and the catalytic ability of natural enzymes. These characteristics give nanozymes significant advantages in pesticide residue detection: High catalytic activity: Nanozymes have catalytic activity similar to that of natural enzymes and can catalyze a variety of chemical reactions, thereby achieving rapid detection of pesticide residues. High stability: Compared with natural enzymes, nanozymes have higher stability and can maintain their catalytic activity under different environmental conditions. Easy to prepare and store: The preparation process of nanozymes is relatively simple, and they are easy to store and transport, which facilitates their wide application in pesticide residue detection. With the continuous development of nanotechnology and biotechnology, pesticide residue detection technology based on nanozymes will continue to improve and innovate, providing a more powerful guarantee for the quality and safety of Chinese herbal medicine solutions.

[0003] Existing pesticide residue detection methods mainly use high pressure to deliver mobile phases, and use special methods to fill the chromatographic column with small particles to improve column efficiency. After the sample is separated on the chromatographic column, it is connected to a highly sensitive detector after the column to continuously detect the effluent, thereby achieving quantitative analysis of pesticide residues.

[0004] For example, a method for detecting pesticide residues in the invention patent announcement with announcement number CN110346342B includes: S1: washing impurities on the outer surface of fruits to be tested for pesticide residues with clean water in advance, and wiping off moisture on the outer surface of the fruits; S2: after pre-cleaning the fruits in S1, placing the objects to be tested for pesticides in a tray inside a pesticide residue detector, and then closing the top cover of the pesticide residue detector; S3: after placing the fruits in the pesticide residue detector in S2, connecting the serial interface of the pesticide residue detector to a computer, and turning on the switch of the pesticide residue detector; S4: after turning on the switch of the pesticide residue detector in S3, using the detection equipment inside the pesticide residue detector to transmit data to the computer, and using the computer's spectral analysis system to identify the pesticide residues on the surface of the fruits.

[0005] For example, a pesticide residue detection system announced in the invention patent with announcement number: CN111965124B includes: a flushing unit, a reaction unit, a detection unit and a waste liquid discharge unit arranged in sequence from top to bottom; the flushing unit includes an electronic scale, a first drive motor, a cleaning bucket, a cleaning basket, a cleaning liquid tank and a water tank; the first drive motor is arranged on the electronic scale, the cleaning bucket is connected to the first drive motor body, the cleaning basket is set in the cleaning bucket and connected to the output shaft of the first drive motor, the cleaning bucket is bidirectionally connected to the cleaning liquid tank, the water tank is respectively connected to the cleaning liquid tank and the reaction unit, the reaction unit is used to perform a reagent reaction on the pesticide residue liquid in the cleaning liquid tank, and the detection unit is used to detect the reaction liquid after the reagent reaction; the waste liquid discharge unit is used for waste liquid treatment and discharge.

[0006] However, in the process of implementing the technical solution of the invention in the embodiments of the present application, the present application found that the above technology has at least the following technical problems:

[0007] In the prior art, since some Chinese medicinal materials solutions contain a large amount of polysaccharides, pigments and other ingredients (such as wolfberry, ginseng, etc.), polysaccharides, pigments and other ingredients may mask or interfere with the detection signal of pesticide residues. Polysaccharides, pigments, etc. may absorb or scatter the light used in the detection process, causing signal weakening or distortion, resulting in obvious interference in the detection process of pesticide residues in Chinese medicinal materials. Summary of the invention

[0008] The embodiments of the present application solve the problem of obvious interference in the detection process of pesticide residues in traditional Chinese medicines in the prior art by providing a method for detecting pesticide residues in traditional Chinese medicines based on nanozymes, thereby achieving more accurate detection of pesticide residues in traditional Chinese medicines.

[0009] The embodiment of the present application provides a method for detecting pesticide residues in traditional Chinese medicines based on nanozymes, comprising the following steps: S1, obtaining a residual interference degree value by obtaining absorbance data and reference absorbance data of a traditional Chinese medicine solution within a preset time period, judging whether to obtain an enzyme reaction effective value based on the residual interference degree value, the residual interference degree value being used to evaluate the interference degree of the interfering substance on the detection of pesticide residues in the traditional Chinese medicine solution, and the absorbance data being used to describe the interference degree of the interfering substance on the detection of pesticide residues in the traditional Chinese medicine solution; S2, if the enzyme reaction effective value is obtained, judging whether to perform enzyme reaction adjustment based on the obtained enzyme reaction effective value, the enzyme reaction adjustment being used to adjust the enzyme reaction effective value to not less than a preset enzyme reaction rate threshold, the enzyme reaction effective value being used to evaluate the effectiveness of the enzyme reaction on the detection of pesticide residues in the traditional Chinese medicine solution; S3, obtaining an enzyme reaction interference value based on the enzyme reaction effective value after the enzyme reaction adjustment and the signal change data, judging whether to obtain the pesticide residue concentration data of a preset detector based on the enzyme reaction interference value, the enzyme reaction interference value being used to evaluate the influence of the enzyme reaction rate on the signal change rate, and the signal change data being used to describe the influence of the enzyme reaction rate on the signal change rate.

[0010] Furthermore, the absorbance data includes absorbance and light wavelength; the preset time period represents the time period in the process of pesticide residue detection in traditional Chinese medicine solution; the reference absorbance data includes absorbance coefficient, preset average absorbance, preset average absorption coefficient threshold, first preset absorbance distribution weight and second preset absorbance distribution weight.

[0011] Furthermore, the specific process of obtaining the residual interference degree value by obtaining the absorbance data of the Chinese medicinal material solution and the reference absorbance data within the preset time period is as follows: Q1, performing a ratio operation on the absorbance and the preset average absorbance obtained from the preset database to obtain the absorbance deviation value; Q2, obtaining the absorption coefficient by combining the absorbance coefficient and the light wavelength, and the absorption coefficient is used to reflect the light absorption efficiency of the Chinese medicinal material solution within the preset time period; Q3, performing a ratio operation on the absorption coefficient and the preset average absorption coefficient threshold obtained from the preset database to obtain the absorption coefficient deviation value; Q4, obtaining the residual interference degree value by combining the absorbance deviation value, the absorption coefficient deviation value, the first preset absorbance allocation weight and the second preset absorbance allocation weight.

[0012] Furthermore, the limiting expression of the residual interference degree value is as follows:

[0013] ;

[0014] ;

[0015] ;

[0016] In the formula, It indicates the residual interference value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. , m represents the number of the preset time point in the preset time period, y represents the total number of preset time points in the preset time period, Indicates the absorbance deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. It represents the light absorption deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. Indicates the absorbance of the Chinese herbal medicine solution at the mth preset time point in the preset time period, represents the wavelength of light of the Chinese herbal medicine solution at the mth preset time point in the preset time period, k represents the absorption coefficient, represents the first preset light absorption distribution weight, represents the second preset light absorption distribution weight, Indicates the preset average absorbance, represents the preset average absorption coefficient threshold, e represents the natural constant, Indicates the preset absorbance deviation value range, Indicates the preset light absorption deviation value range.

[0017] Furthermore, the specific process of judging whether to obtain the effective value of the enzyme reaction based on the residual interference degree value is as follows: judging whether the residual interference degree value is not higher than the preset absorbance interference threshold value; when the residual interference degree value is not higher than the preset absorbance interference threshold value, the effective value of the enzyme reaction is not obtained and the pesticide residue concentration data of the preset detector is directly obtained; when the residual interference degree value is higher than the preset absorbance interference threshold value, the effective value of the enzyme reaction is obtained by combining the enzyme reaction data and the reaction rate constant; the enzyme reaction data includes the nanozyme concentration and the Chinese medicinal material solution concentration.

[0018] Furthermore, the specific process of obtaining the effective value of the enzyme reaction is as follows: W1, multiplying the enzyme reaction data and the reaction rate constant obtained from the preset database to obtain an enzyme reaction rate threshold, and the enzyme reaction rate threshold is used to reflect the enzyme reaction rate within a preset time period; W2, performing a ratio operation on the enzyme reaction rate threshold and the preset average enzyme reaction rate threshold obtained from the preset database to obtain an enzyme reaction rate deviation value, and the enzyme reaction rate deviation value is used to reflect the degree of deviation of the enzyme reaction rate within a preset time period; W3, combining the enzyme reaction rate deviation value and the residual interference degree value of the traditional Chinese medicine solution in the preset time period to obtain the effective value of the enzyme reaction.

[0019] Furthermore, the specific process of judging whether to adjust the enzyme reaction based on the obtained enzyme reaction effective value is as follows: V1, judging whether the enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is not performed and the pesticide residue concentration data of the preset detector is directly obtained. Otherwise, V2 is executed; V2, sending a prompt to the preset personnel to increase the reaction temperature to the preset temperature maximum value by a preset multiple. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is stopped. Otherwise, V3 is executed; V3, sending a prompt to the preset personnel to adjust the reaction pH value to the preset average pH threshold. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is stopped. Otherwise, an alarm prompt is sent.

[0020] Furthermore, the signal change data includes interference signal intensity and target signal intensity; the interference signal intensity represents the intensity of the quantitative signal of the interference target signal generated by the interfering substances contained in the Chinese medicinal materials themselves within a preset time period; the target signal intensity represents the intensity of the quantitative signal generated by the reaction of pesticide residues in the Chinese medicinal materials solution with enzymes within a preset time period.

[0021] Furthermore, the specific process of obtaining the enzyme reaction interference value based on the enzyme reaction effective value and signal change data after enzyme reaction adjustment is as follows: E1, performing a ratio operation on the interference signal intensity and the target signal intensity to obtain a signal interference ratio, and the signal interference ratio is used to reflect the degree of influence of the interfering substance on the detection signal within a preset time period; E2, performing a ratio operation on the signal interference ratio and a preset average signal interference ratio threshold obtained from a preset database to obtain a signal interference ratio deviation value, and the signal interference ratio deviation value is used to reflect the deviation of the influence of the interfering substance on the detection signal within a preset time period; E3, combining the signal interference ratio deviation value and the enzyme reaction effective value of the traditional Chinese medicine solution in the preset time period to obtain the enzyme reaction interference value.

[0022] One or more technical solutions provided in the embodiments of the present application have at least the following technical effects or advantages:

[0023] 1. The residual interference degree value is obtained by obtaining the absorbance data of the Chinese medicinal material solution and the reference absorbance data and judging whether to obtain the effective value of the enzyme reaction, and then judging whether to adjust the enzyme reaction based on the obtained effective value of the enzyme reaction, and finally, the enzyme reaction interference value is obtained based on the effective value of the enzyme reaction after the enzyme reaction adjustment and the signal change data and judging whether to obtain the pesticide residue concentration data of the preset detector, thereby realizing the effective detection of pesticide residues in the Chinese medicinal material solution, and then realizing more accurate detection of pesticide residues in Chinese medicinal materials, and effectively solving the problem of obvious interference in the detection process of pesticide residues in Chinese medicinal materials in the prior art.

[0024] 2. The residual interference degree value is obtained through the absorbance deviation value, the absorption coefficient deviation value, the first preset absorbance distribution weight and the second preset absorbance distribution weight, and then the enzyme reaction effective value is obtained by combining the enzyme reaction rate deviation value and the residual interference degree value of the traditional Chinese medicine solution in the preset time period. Finally, the enzyme reaction interference value is obtained by combining the signal interference ratio deviation value and the enzyme reaction effective value of the traditional Chinese medicine solution in the preset time period, thereby realizing the accurate quantification of the detection of pesticide residues in the traditional Chinese medicine solution, and then realizing the improvement of the reliability of the detection of pesticide residues in the traditional Chinese medicine solution.

[0025] 3. By judging whether the effective value of the enzyme reaction is not lower than the preset enzyme reaction rate threshold, when the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold, and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is not performed and the pesticide residue concentration data of the preset detector is directly obtained, thereby realizing the quantification of the influence of the monitored enzyme reaction on the detection of pesticide residues in Chinese medicinal materials, and then realizing the improvement of the effectiveness of the detection of pesticide residues in Chinese medicinal materials solutions. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A flow chart of a method for detecting pesticide residues in traditional Chinese medicines based on nanozymes provided in an embodiment of the present application;

[0027] Figure 2 An overall flow chart provided for the embodiments of the present application;

[0028] Figure 3 A statistical graph of changes in the residual interference level values ​​provided in an embodiment of the present application. DETAILED DESCRIPTION

[0029] The embodiment of the present application solves the problem of obvious interference in the detection process of pesticide residues in traditional Chinese medicines in the prior art by providing a method for detecting pesticide residues in traditional Chinese medicines based on nanozymes. The residual interference degree value is obtained by obtaining the absorbance data of the traditional Chinese medicine solution and the reference absorbance data. Based on the residual interference degree value, it is judged whether to obtain the effective value of the enzyme reaction. Then, based on the obtained effective value of the enzyme reaction, it is judged whether to adjust the enzyme reaction. Finally, according to the effective value of the enzyme reaction after the adjustment and the signal change data, the enzyme reaction interference value is obtained. Based on the enzyme reaction interference value, it is judged whether to obtain the pesticide residue concentration data of the preset detector, thereby achieving more accurate detection of pesticide residues in traditional Chinese medicines.

[0030] The technical solution in the embodiment of the present application is to solve the problem of obvious interference in the above-mentioned detection process of pesticide residues in Chinese herbal medicines. The overall idea is as follows:

[0031] The residual interference degree value is obtained through the absorbance data of the Chinese medicinal material solution and the reference absorbance data, and it is judged whether to obtain the effective value of the enzyme reaction, and then it is judged whether to adjust the enzyme reaction based on the obtained effective value of the enzyme reaction. Finally, the enzyme reaction interference value is obtained based on the effective value of the enzyme reaction after the enzyme reaction adjustment and the signal change data, and it is judged whether to obtain the pesticide residue concentration data of the preset detector, thereby achieving a more accurate effect of detecting pesticide residues in Chinese medicinal materials.

[0032] In order to better understand the above technical solution, the above technical solution will be described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0033] like Figure 1As shown, it is a flow chart of the method for detecting pesticide residues in Chinese medicinal materials based on nanozymes provided in an embodiment of the present application, and the method comprises the following steps: S1, obtaining a residual interference degree value: obtaining a residual interference degree value by obtaining absorbance data of a Chinese medicinal material solution and reference absorbance data within a preset time period, and judging whether to obtain an enzyme reaction effective value based on the residual interference degree value, the residual interference degree value is used to evaluate the interference degree of the interfering substance on the detection of pesticide residues in the Chinese medicinal material solution, and the absorbance data is used to describe the interference degree of the interfering substance on the detection of pesticide residues in the Chinese medicinal material solution; S2, obtaining an enzyme reaction effective value: if the enzyme reaction effective value is obtained, then based on the obtained The effective value of the enzyme reaction is used to determine whether to adjust the enzyme reaction. The enzyme reaction adjustment is used to adjust the effective value of the enzyme reaction to not less than a preset enzyme reaction rate threshold. The effective value of the enzyme reaction is used to evaluate the effectiveness of the enzyme reaction in detecting pesticide residues in traditional Chinese medicine solutions; S3, obtaining the enzyme reaction interference value: the enzyme reaction interference value is obtained based on the effective value of the enzyme reaction after the enzyme reaction adjustment and the signal change data. Based on the enzyme reaction interference value, it is determined whether to obtain the pesticide residue concentration data of the preset detector. The enzyme reaction interference value is used to evaluate the influence of the enzyme reaction rate on the signal change rate. The signal change data is used to describe the influence of the enzyme reaction rate on the signal change rate.

[0034] like Figure 2 As shown, it is the overall flow chart provided in the embodiment of the present application. The higher the residual interference degree value, the more interfering substances exist in the Chinese medicinal material solution, such as non-specific components such as polysaccharides and pigments, which may cause non-specific reactions, which may affect the specificity and efficiency of the enzyme reaction, thereby reducing the effective value of the enzyme reaction. The lower the effective value of the enzyme reaction, the more likely the enzyme reaction is to be inhibited. The higher the enzyme reaction interference value, the greater the impact of the change in the enzyme reaction rate on the signal change rate, which may be due to the interfering substances in the Chinese medicinal material solution or the instability of the enzyme reaction conditions.

[0035] The process of detecting pesticide residues in traditional Chinese medicines by absorbance measurement is as follows: injecting the traditional Chinese medicine solution into a spectrometer, measuring the absorbance (different types of pesticides have different absorbances at specific wavelengths), recording the measured absorbance of the pesticide, using a standard curve to convert the absorbance value into the concentration of the pesticide, and establishing a linear relationship between the absorbance and concentration of a pesticide solution of known concentration by the standard curve method to deduce the concentration of a pesticide solution of unknown concentration in the traditional Chinese medicine solution, thereby obtaining pesticide residue concentration data, which includes the concentrations corresponding to different types of pesticides in the traditional Chinese medicine solution; thus achieving more accurate detection of pesticide residues in traditional Chinese medicines.

[0036] It should be supplemented that the absorbance data includes absorbance and light wavelength; the preset time period represents the time period in the process of pesticide residue detection in Chinese medicinal material solution; the reference absorbance data includes absorbance coefficient, preset average absorbance, preset average absorption coefficient threshold, first preset absorbance allocation weight and second preset absorbance allocation weight; the first preset absorbance allocation weight is used to evaluate the influence of absorbance on the residual interference degree value within the preset time period; the second preset absorbance allocation weight is used to evaluate the influence of light absorption efficiency on the residual interference degree value within the preset time period.

[0037] Specifically, impurities such as polysaccharides and pigments may absorb light of specific wavelengths. The light wavelengths of interfering substances and different types of pesticides are measured by a spectrometer, and the absorbance of interfering substances at preset time points is measured by a UV-visible spectrophotometer. The absorbance coefficient is represented by the average value of the absorbance coefficients of interfering substances in the historical time period in the preset database, the preset average absorbance is represented by the average value of the absorbances of interfering substances in the historical time period in the preset database, and the preset average absorption coefficient threshold is represented by the average value of the absorption coefficients of interfering substances in the historical time period in the preset database; thereby achieving more accurate detection of pesticide residues in traditional Chinese medicines.

[0038] Specifically, the sum of the first preset absorbance distribution weight and the second preset absorbance distribution weight is 1, for example, the first preset absorbance distribution weight is 0.5, and the first preset absorbance distribution weight is 0.5; the first preset absorbance distribution weight is the weight corresponding to the absorbance value of the interfering substance preset in the preset database, which represents the value of the influence of the absorbance value of the interfering substance on the residual interference degree value. When used, the weight corresponding to the preset absorbance value of the interfering substance can be directly obtained from the preset database, and the corresponding relationship can be a pre-set mapping relationship. For example, the absorbance of the interfering substance in the training set of the pesticide residue detection of traditional Chinese medicine based on absorbance and the weight corresponding to the absorbance value of the interfering substance preset in the preset database form a mapping set, and the real-time absorbance of the interfering substance is input into the mapping set to obtain the corresponding weight, wherein the mapping relationship can be a one-to-one correspondence or a many-to-one relationship. In this example, its value range is [0, 1].

[0039] Furthermore, the specific process of obtaining the residual interference degree value by obtaining the absorbance data of the Chinese herbal medicine solution and the reference absorbance data within the preset time period is as follows: Q1, performing a ratio operation on the absorbance and the preset average absorbance obtained from the preset database to obtain the absorbance deviation value (i.e., the residual interference degree value in the restriction expression) ), the absorbance deviation value is used to reflect the absorbance deviation of the Chinese herbal medicine solution within a preset time period; Q2, combining the absorbance coefficient and the light wavelength to obtain the absorption coefficient, the absorption coefficient is used to reflect the light absorption efficiency of the Chinese herbal medicine solution within a preset time period; Q3, the absorption coefficient is compared with the preset average absorption coefficient threshold obtained from the preset database to obtain the absorption coefficient deviation value (that is, the residual interference degree value in the limiting expression ), the absorption coefficient deviation value is used to reflect the deviation of the light absorption efficiency of the Chinese medicinal material solution within a preset time period; Q4, combining the absorbance deviation value, the absorption coefficient deviation value, the first preset absorbance distribution weight and the second preset absorbance distribution weight to obtain the residual interference degree value.

[0040] Among them, the limiting expression of the residual interference degree value is as follows:

[0041] ;

[0042] ;

[0043] ;

[0044] In the formula, It indicates the residual interference value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. , m represents the number of the preset time point in the preset time period, y represents the total number of preset time points in the preset time period, Indicates the absorbance deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. It represents the light absorption deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. Indicates the absorbance of the Chinese herbal medicine solution at the mth preset time point in the preset time period, represents the wavelength of light of the Chinese herbal medicine solution at the mth preset time point in the preset time period, k represents the absorption coefficient, represents the first preset light absorption distribution weight, represents the second preset light absorption distribution weight, Indicates the preset average absorbance, represents the preset average absorption coefficient threshold, e represents the natural constant, Indicates the preset absorbance deviation value range, Indicates the preset light absorption deviation value range.

[0045] In this embodiment, the algorithm of this embodiment combines the absorbance data for comprehensive analysis to obtain the residual interference degree value. The absorbance data in the algorithm of this embodiment does not exist independently, but are interrelated. A higher absorbance does not mean a lower residual interference degree value, and the influence of the wavelength of light should also be considered comprehensively. The wavelength of light determines the energy and penetration of light. When light of different wavelengths passes through a Chinese medicinal material solution, its absorbance may be different. Interfering substances absorb light of specific wavelengths, thereby affecting the absorbance of the Chinese medicinal material solution. The absorbance of interfering substances is high, which may reduce the absorbance of pesticides in the Chinese medicinal material solution, making the detection of pesticides difficult. The parameters of the algorithm of this embodiment need to consider the impact on the results together.

[0046] It should be understood that the preset absorbance interference threshold is represented by the variance of the absorbance of the interfering substance in the historical time period in the preset database. The range is generally ,when When it is greater than 1, it means that the absorbance of the interfering substance exceeds the preset average absorbance, which will reduce the absorbance of the pesticide in the Chinese herbal medicine solution, making the measurement result inaccurate. The range is generally ,when When it is greater than 1, it means that the light absorption coefficient of the interfering substance exceeds the preset average absorption coefficient threshold, which will reduce the light absorption efficiency of the pesticide in the Chinese medicinal material solution and make the measurement result inaccurate.

[0047] Specifically, assuming the absorbance deviation value The range is 0.1-1, the light absorption deviation value The range is 0.1-1, the first preset absorption distribution weight Fixed to 0.5, the second preset absorption distribution weight Fixed to 0.5, such as Figure 3 As shown in the figure, it is a statistical diagram of the residual interference degree value provided in the embodiment of the present application. It can be seen from the figure that when the absorbance deviation value When fixed at 0.5, the light absorption deviation value The value of residual interference degree gradually increases and gradually decreases, which realizes the quantification of the interference degree of interfering substances on the detection of pesticide residues in Chinese medicinal materials solution, and thus realizes more accurate detection of pesticide residues in Chinese medicinal materials.

[0048] Furthermore, the specific process of determining whether to obtain the effective value of the enzyme reaction based on the residual interference degree value is as follows: determine whether the residual interference degree value is not higher than the preset absorbance interference threshold value. When the residual interference degree value is not higher than the preset absorbance interference threshold value, it is considered that the residual interference has a small impact on the enzyme reaction rate, and the effective value of the enzyme reaction is not obtained, and the pesticide residue concentration data of the preset detector is directly obtained through the absorbance measurement method; when the residual interference degree value is higher than the preset absorbance interference threshold value, the effective value of the enzyme reaction is obtained by combining the enzyme reaction data and the reaction rate constant; the enzyme reaction data includes the nanozyme concentration and the Chinese medicinal material solution concentration.

[0049] Specifically, the specific process of obtaining the effective value of the enzyme reaction is as follows: W1, the enzyme reaction data and the reaction rate constant obtained from the preset database are multiplied to obtain the enzyme reaction rate threshold, and the enzyme reaction rate threshold is used to reflect the enzyme reaction rate within a preset time period; W2, the enzyme reaction rate threshold and the preset average enzyme reaction rate threshold obtained from the preset database are ratioed to obtain the enzyme reaction rate deviation value (that is, the enzyme reaction rate deviation value in the restriction expression of the effective value of the enzyme reaction ), the enzyme reaction rate deviation value is used to reflect the degree of deviation of the enzyme reaction rate within a preset time period; W3, combining the enzyme reaction rate deviation value and the residual interference degree value of the traditional Chinese medicine solution in the preset time period to obtain the enzyme reaction effective value.

[0050] Among them, the limiting expression of the effective value of the enzyme reaction is as follows:

[0051] ;

[0052] ;

[0053] In the formula, It represents the effective value of the enzyme reaction of the Chinese herbal medicine solution at the mth preset time point in the preset time period. , m represents the number of the preset time point in the preset time period, y represents the total number of preset time points in the preset time period, Indicates the residual interference value of the Chinese medicinal material solution corresponding to the residual interference value at the mth preset time point in the preset time period being higher than the preset absorbance interference threshold, It represents the enzyme reaction rate deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. represents the nanozyme concentration at the mth preset time point in the preset time period, Indicates the concentration of the Chinese medicinal material solution at the mth preset time point in the preset time period, represents the reaction rate constant, e represents the natural constant, represents the preset average enzyme reaction rate threshold, Indicates the preset enzyme reaction rate deviation value range.

[0054] In this embodiment, the preset average enzyme reaction rate threshold is represented by the average value of the enzyme reaction rate in the historical time period in the preset database, and the nanozyme concentration and the concentration of the Chinese medicinal material solution are measured by colorimetry, that is, by drawing a standard curve, that is, a curve showing the relationship between color intensity and nanozyme concentration. For example, after measuring the color intensity of the nanozyme solution to be tested after reacting with the same reagent by a photometer, the nanozyme concentration corresponding to the color intensity is obtained according to the drawn standard curve.

[0055] The range is generally ,when When it is greater than 1, it means that the enzyme reaction rate of the Chinese medicinal material solution exceeds the preset average enzyme reaction rate threshold, which will inhibit the enzyme activity of the Chinese medicinal material solution and make the measurement result inaccurate.

[0056] It should be understood that the algorithm of this embodiment combines the enzyme reaction data for comprehensive analysis to obtain the effective value of the enzyme reaction. The enzyme reaction data in the algorithm of this embodiment does not exist independently, and there is a correlation between them. The higher the nanozyme concentration and the concentration of the Chinese herbal medicine solution, the higher the effective value of the enzyme reaction. The influence of the residual interference degree value should also be considered comprehensively. As a catalyst, the concentration of nanozyme directly affects the enzyme reaction rate and efficiency. When the concentration of nanozyme increases, the enzyme reaction may be more rapid and complete, which helps to more accurately detect the pesticide residues in the Chinese herbal medicine solution, but the higher the concentration of nanozyme may cause the interference substances to interfere with the pesticide detection, which in turn leads to an increase in the residual interference degree value. The higher the concentration of the Chinese herbal medicine solution, the more likely it is that the enzyme's processing capacity will be exceeded, resulting in the generation of interfering substances and an increase in the residual interference degree value. The parameters of the algorithm of this embodiment need to consider the impact on the results together; the effectiveness of the quantitative enzyme reaction on the detection of pesticide residues in the Chinese herbal medicine solution is achieved, and then more accurate detection of pesticide residues in Chinese herbal medicines is achieved.

[0057] Furthermore, the specific process of judging whether to adjust the enzyme reaction based on the obtained enzyme reaction effective value is as follows: V1, judging whether the enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is not performed and the pesticide residue concentration data of the preset detector is directly obtained. Otherwise, V2 is executed; V2, sending a prompt to the preset personnel to increase the reaction temperature to the preset temperature maximum value by a preset multiple. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is stopped. Otherwise, V3 is executed; V3, sending a prompt to the preset personnel to adjust the reaction pH value to the preset average pH threshold. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is stopped. Otherwise, an alarm prompt is sent.

[0058] In this embodiment, the preset enzyme reaction rate threshold is represented by the variance of the enzyme reaction rate in the historical time period in the preset database, the preset temperature maximum value is represented by the maximum value of the enzyme reaction temperature in the historical time period in the preset database, and the preset average pH threshold is represented by the average value of the enzyme reaction pH value in the historical time period in the preset database. The reaction pH value can be adjusted to the preset average pH threshold by increasing or decreasing the pH value until the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold, and the residual interference degree value is not higher than the preset absorbance interference threshold. The reaction temperature and pH value are adjusted by monitoring the enzyme reaction effective value and the residual interference degree value, so as to optimize the enzyme reaction conditions, thereby obtaining more accurate pesticide residue concentration data; and more accurate detection of pesticide residues in traditional Chinese medicines is achieved.

[0059] Furthermore, the signal change data includes interference signal strength and target signal strength; the interference signal strength represents the strength of the quantitative signal of the interference target signal generated by the interfering substances contained in the Chinese medicinal materials themselves within a preset time period; the target signal strength represents the strength of the quantitative signal generated by the reaction of pesticide residues in the Chinese medicinal materials solution with the enzyme within a preset time period; the interference signal strength and the target signal strength are measured by a signal strength tester.

[0060] Specifically, the specific process of obtaining the enzyme reaction interference value based on the enzyme reaction effective value and signal change data after the enzyme reaction is adjusted is as follows: E1, performing a ratio operation on the interference signal intensity and the target signal intensity to obtain a signal interference ratio, and the signal interference ratio is used to reflect the degree of influence of the interfering substance on the detection signal within a preset time period; E2, performing a ratio operation on the signal interference ratio and a preset average signal interference ratio threshold obtained from a preset database to obtain a signal interference ratio deviation value (i.e., the value in the restriction expression of the enzyme reaction interference value) ), the signal-interference ratio deviation value is used to reflect the deviation of the influence of the interfering substance on the detection signal within a preset time period; E3, combining the signal-interference ratio deviation value and the enzyme reaction effective value of the traditional Chinese medicine solution in the preset time period to obtain the enzyme reaction interference value.

[0061] Among them, the limiting expression of the enzyme reaction interference value is as follows:

[0062] ;

[0063] ;

[0064] In the formula, It represents the enzyme reaction interference value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. , m represents the number of the preset time point in the preset time period, y represents the total number of preset time points in the preset time period, Indicates the effective value of the enzyme reaction of the Chinese medicinal material solution corresponding to the enzyme reaction rate value of the mth preset time period is not higher than the preset enzyme reaction interference threshold, It represents the signal-to-interference ratio deviation value of the Chinese medicinal material solution at the mth preset time point in the preset time period. It indicates the interference signal strength of the Chinese herbal medicine solution at the mth preset time point in the preset time period. Indicates the target signal strength of the Chinese herbal medicine solution at the mth preset time point in the preset time period, represents the preset average signal-to-interference ratio threshold, e represents a natural constant, Indicates the preset signal-to-interference ratio deviation value range.

[0065] The preset average signal-to-interference ratio threshold is represented by the average value of the signal strength of the interfering substance in the historical time period in the preset database.

[0066] The range is generally ,when When it is greater than 1, it means that the signal-to-interference ratio of the Chinese medicinal material solution exceeds the preset average signal-to-interference ratio threshold, which will mask the target signal of the Chinese medicinal material solution and make the measurement result inaccurate.

[0067] It should be understood that the algorithm of this embodiment combines the signal change data to comprehensively analyze the enzyme reaction interference value. In the algorithm of this embodiment, the signal change data does not exist independently, and there is mutual correlation between them. The higher the interference signal intensity, the higher the enzyme reaction interference value. The influence of the target signal intensity and the enzyme reaction effective value should also be considered comprehensively. The interfering substance may compete with the pesticide residue for the catalytic site of the enzyme, thereby affecting the accurate detection of the target signal. When the target signal intensity is higher, more pesticide residues are catalyzed and converted by the enzyme, which may cause the enzyme reaction effective value to increase. The parameters of the algorithm of this embodiment need to be considered together and at the same time. The impact on the result.

[0068] Specifically, assuming that the signal-to-interference ratio deviation value The range is 0.1-1, which is not higher than the effective value of the enzyme reaction at the preset enzyme reaction interference threshold. The range is 0.5-1, as shown in Table 1, which is a statistical table of changes in the enzyme reaction interference value provided in the embodiment of the present application:

[0069] Table 1 Statistics of changes in enzyme reaction interference values

[0070]

[0071] From the above table, we can see that as the signal-to-interference ratio deviation value increases The gradually increasing value of the enzyme reaction and the effective value of the enzyme reaction not exceeding the preset enzyme reaction interference threshold The enzyme reaction interference value The gradual increase means that the influence of enzyme reaction rate on signal change rate gradually increases; the influence of enzyme reaction rate on signal change rate is quantified, thereby achieving more accurate detection of pesticide residues in traditional Chinese medicine.

[0072] Furthermore, the specific process of determining whether to obtain the pesticide residue concentration data of the preset detector based on the enzyme reaction interference value is as follows: U1, determining whether the enzyme reaction interference value is not higher than the preset signal interference threshold. When the enzyme reaction interference value is not higher than the preset signal interference threshold, and the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the pesticide residue concentration data of the preset detector is directly obtained, otherwise U2 is executed; U2, sending a reminder to the preset personnel to dilute the concentration of the Chinese medicinal material solution. When the monitored enzyme reaction interference value is not higher than the preset signal interference threshold, the monitored When the effective value of the enzyme reaction is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the execution is stopped; otherwise, U3 is executed to dilute the concentration of the Chinese medicinal material solution to reduce the concentration of the interfering substance, thereby reducing its interference with the enzyme reaction; U3 is used for denoising. When the monitored enzyme reaction interference value is not higher than the preset signal interference threshold, and at the same time, the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the execution is stopped; otherwise, an alarm is sent. The denoising process means removing the signal generated by the interfering substance through the denoising algorithm.

[0073] In this embodiment, the preset signal interference threshold is represented by the variance of the signal intensity of the interfering substance in the historical time period in the preset database. Through the dilution method and denoising algorithm, such as the dilution solvent can be water, alcohol, ether, etc., the denoising algorithm in this example adopts the median filtering algorithm. The median filtering algorithm can replace the value of each sampling point with the median in the signal window, which can improve the reliability of pesticide residue detection in traditional Chinese medicine, thereby achieving more accurate pesticide residue detection in traditional Chinese medicine.

[0074] In summary, the embodiment of the present application obtains the absorbance data of the Chinese medicinal material solution and the reference absorbance data to obtain the residual interference degree value and judges whether to obtain the effective value of the enzyme reaction, and then judges whether to adjust the enzyme reaction based on the obtained effective value of the enzyme reaction, and finally obtains the enzyme reaction interference value based on the effective value of the enzyme reaction after the enzyme reaction adjustment and the signal change data, and judges whether to obtain the pesticide residue concentration data of the preset detector, thereby realizing effective detection of pesticide residues in the Chinese medicinal material solution, and then realizing more accurate detection of pesticide residues in Chinese medicinal materials, and effectively solving the problem of obvious interference in the detection process of pesticide residues in Chinese medicinal materials in the prior art.

[0075] It will be appreciated by those skilled in the art that embodiments of the present invention may be provided as methods, systems, or computer program products. Therefore, the present invention may take the form of a complete hardware embodiment, a complete software embodiment, or an embodiment combining software and hardware. Furthermore, the present invention may take the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) containing computer-usable program code.

[0076] The present invention is described with reference to flowcharts and / or block diagrams of methods, devices (systems), and computer program products according to embodiments of the present invention. It should be understood that each process and / or block in the flowchart and / or block diagram, as well as the combination of processes and / or blocks in the flowchart and / or block diagram, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to produce a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the processes in the flowchart and / or block diagram. Figure 1 A process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0077] These computer program instructions may also be stored in a computer-readable memory capable of directing a computer or other programmable data processing device to operate in a specific manner, so that the instructions stored in the computer-readable memory produce an article of manufacture comprising an instruction device, which implements the process Figure 1 A process or multiple processes and / or boxes Figure 1 A function specified in one or more boxes.

[0078] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operating steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing instructions for implementing the process in the computer or other programmable device. Figure 1 A process or multiple processes and / or boxes Figure 1 A step that specifies a function in one or more boxes.

[0079] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0080] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for detecting pesticide residues in traditional Chinese medicine based on nanozymes, characterized in that: The following steps are involved: S1, obtaining a residual interference degree value by obtaining absorbance data of a Chinese medicinal material solution and a reference absorbance data within a preset time period, and judging whether to obtain an enzyme reaction effective value based on the residual interference degree value, wherein the residual interference degree value is used to evaluate the interference degree of the interfering substance on the detection of pesticide residues in the Chinese medicinal material solution, and the absorbance data is used to describe the interference degree of the interfering substance on the detection of pesticide residues in the Chinese medicinal material solution; S2, if the enzyme reaction effective value is obtained, then judging whether to perform enzyme reaction adjustment based on the obtained enzyme reaction effective value, wherein the enzyme reaction adjustment is used to adjust the enzyme reaction effective value to not less than a preset enzyme reaction rate threshold, and the enzyme reaction effective value is used to evaluate the effectiveness of the enzyme reaction for the detection of pesticide residues in traditional Chinese medicine solutions; S3, obtaining an enzyme reaction interference value based on the enzyme reaction effective value after the enzyme reaction adjustment and the signal change data, and judging whether to obtain the pesticide residue concentration data of the preset detector based on the enzyme reaction interference value, wherein the enzyme reaction interference value is used to evaluate the influence of the enzyme reaction rate on the signal change rate, and the signal change data is used to describe the influence of the enzyme reaction rate on the signal change rate; The limiting expression of the residual interference degree value is as follows: In the formula, represents the residual interference value of the Chinese herbal medicine solution at the mth preset time point in the preset time period, m = 1, 2, ..., y, m represents the number of the preset time point in the preset time period, y represents the total number of preset time points in the preset time period, XG m Indicates the absorbance deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period, XS m It represents the light absorption deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period. Indicates the absorbance of the Chinese herbal medicine solution at the mth preset time point in the preset time period, represents the wavelength of light of the Chinese herbal medicine solution at the mth preset time point in the preset time period, k represents the absorption coefficient, θ1 represents the first preset absorption distribution weight, θ2 represents the second preset absorption distribution weight, represents the preset average absorbance, XS0 represents the preset average absorption coefficient threshold, e represents the natural constant, ΔXG m Indicates the preset absorbance deviation range, ΔXS m Indicates the preset light absorption deviation value range; The limiting expression for the effective value of the enzyme reaction is as follows: In the formula, represents the effective value of the enzyme reaction of the Chinese herbal medicine solution at the mth preset time point in the preset time period, m=1,2,...,y, m represents the number of the preset time point in the preset time period, y represents the total number of the preset time points in the preset time period, Indicates the residual interference value of the Chinese herbal medicine solution corresponding to the residual interference value at the mth preset time point in the preset time period that is higher than the preset absorbance interference threshold, SL m It represents the enzyme reaction rate deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period, M m represents the nanozyme concentration at the mth preset time point in the preset time period, ZY m represents the concentration of the Chinese medicinal material solution at the mth preset time point in the preset time period, v represents the reaction rate constant, e represents the natural constant, SL0 represents the preset average enzyme reaction rate threshold, ΔSL m Indicates the preset enzyme reaction rate deviation value range; The limiting expression of the enzyme reaction interference value is as follows: In the formula, represents the enzyme reaction interference value of the Chinese herbal medicine solution at the mth preset time point in the preset time period, m=1,2,...,y, m represents the number of the preset time point in the preset time period, y represents the total number of the preset time points in the preset time period, Indicates the effective value of the enzyme reaction of the Chinese herbal medicine solution corresponding to the enzyme reaction rate value of the mth preset time period is not higher than the preset enzyme reaction interference threshold, XH m Indicates the signal-to-interference ratio deviation value of the Chinese herbal medicine solution at the mth preset time point in the preset time period, RX m Indicates the interference signal strength of the Chinese herbal medicine solution at the mth preset time point in the preset time period, MX m represents the target signal strength of the Chinese herbal medicine solution at the mth preset time point in the preset time period, XH0 represents the preset average signal-to-interference ratio threshold, e represents the natural constant, ΔXH m Indicates the preset signal-to-interference ratio deviation value range.

2. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes according to claim 1, characterized in that: The absorbance data include absorbance and light wavelength; The preset time period represents the time period during the detection of pesticide residues in the Chinese herbal medicine solution; The reference absorbance data includes an absorbance coefficient, a preset average absorbance, a preset average absorbance coefficient threshold, a first preset absorbance distribution weight, and a second preset absorbance distribution weight.

3. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 2, characterized in that: The specific process of obtaining the residual interference degree value by acquiring the absorbance data of the Chinese medicinal material solution and the reference absorbance data within the preset time period is as follows: Q1, performing a ratio operation between the absorbance and a preset average absorbance obtained from a preset database to obtain an absorbance deviation value; Q2, combining the light absorption coefficient and the light wavelength to obtain the absorption coefficient, which is used to reflect the light absorption efficiency of the Chinese herbal medicine solution within a preset time period; Q3, performing a ratio operation on the absorption coefficient and a preset average absorption coefficient threshold obtained from a preset database to obtain a light absorption deviation value; Q4, obtaining a residual interference degree value by combining the absorbance deviation value, the light absorption deviation value, the first preset light absorption distribution weight and the second preset light absorption distribution weight.

4. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 3, characterized in that: The specific process of determining whether to obtain the effective value of the enzyme reaction based on the residual interference degree value is as follows: Determine whether the residual interference degree value is not higher than the preset absorbance interference threshold value. When the residual interference degree value is not higher than the preset absorbance interference threshold value, the effective value of the enzyme reaction is not obtained and the pesticide residue concentration data of the preset detector is directly obtained; When the residual interference degree value is higher than the preset absorbance interference threshold, the enzyme reaction effective value is obtained by combining the enzyme reaction data and the reaction rate constant; The enzyme reaction data include nanozyme concentration and Chinese medicinal material solution concentration.

5. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 4, characterized in that: The specific process of obtaining the effective value of the enzyme reaction is as follows: W1, performing a product operation on the enzyme reaction data and the reaction rate constant obtained from the preset database to obtain an enzyme reaction rate threshold, wherein the enzyme reaction rate threshold is used to reflect the enzyme reaction rate within a preset time period; W2, performing a ratio operation on the enzyme reaction rate threshold and the preset average enzyme reaction rate threshold obtained from the preset database to obtain an enzyme reaction rate deviation value, wherein the enzyme reaction rate deviation value is used to reflect the degree of deviation of the enzyme reaction rate within a preset time period; W3, combining the enzyme reaction rate deviation value and the residual interference degree value of the Chinese medicinal material solution in a preset time period to obtain the enzyme reaction effective value.

6. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 5, characterized in that: The specific process of judging whether to adjust the enzyme reaction based on the obtained enzyme reaction effective value is as follows: V1, judging whether the effective value of the enzyme reaction is not lower than the preset enzyme reaction rate threshold. When the monitored effective value of the enzyme reaction is not lower than the preset enzyme reaction rate threshold and the residual interference value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is not performed and the pesticide residue concentration data of the preset detector is directly obtained. Otherwise, V2 is executed; V2, sending a prompt to the preset personnel to increase the reaction temperature to the preset maximum temperature by a preset multiple. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold, and the residual interference degree value is not higher than the preset absorbance interference threshold, the enzyme reaction adjustment is stopped, otherwise V3 is executed; V3, sends a reminder to the preset personnel to adjust the reaction pH value to the preset average pH threshold. When the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold, and the residual interference degree value is not higher than the preset absorbance interference threshold, stop the enzyme reaction adjustment, otherwise send an alarm prompt.

7. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes according to claim 1, characterized in that: The signal change data includes interference signal strength and target signal strength; The interference signal strength represents the strength of the quantified signal of the interference target signal generated by the interference substances contained in the Chinese medicinal materials themselves within a preset time period; The target signal intensity represents the intensity of the quantitative signal generated by the reaction between the pesticide residues in the Chinese medicinal material solution and the enzyme within a preset time period.

8. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 7, characterized in that: The specific process of obtaining the enzyme reaction interference value based on the enzyme reaction effective value and signal change data after the enzyme reaction adjustment is as follows: E1, performing a ratio operation on the interference signal strength and the target signal strength to obtain a signal-to-interference ratio, wherein the signal-to-interference ratio is used to reflect the degree of influence of the interference substance on the detection signal within a preset time period; E2, performing a ratio operation on the signal-to-interference ratio and a preset average signal-to-interference ratio threshold obtained from a preset database to obtain a signal-to-interference ratio deviation value, wherein the signal-to-interference ratio deviation value is used to reflect the deviation of the influence of the interfering substance on the detection signal within a preset time period; E3, combining the signal-to-interference ratio deviation value and the effective value of the enzyme reaction of the Chinese medicinal material solution in a preset time period to obtain the enzyme reaction interference value.

9. The method for detecting pesticide residues in traditional Chinese medicines based on nanozymes as claimed in claim 8, characterized in that: The specific process of determining whether to obtain the pesticide residue concentration data of the preset detector based on the enzyme reaction interference value is as follows: U1, judge whether the enzyme reaction interference value is not higher than the preset signal interference threshold. When the enzyme reaction interference value is not higher than the preset signal interference threshold, and the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, directly obtain the pesticide residue concentration data of the preset detector, otherwise execute U2; U2, sending a reminder to the preset personnel to dilute the concentration of the Chinese herbal medicine solution. When the monitored enzyme reaction interference value is not higher than the preset signal interference threshold, and the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, stop executing, otherwise execute U3; U3, performs denoising processing. When the monitored enzyme reaction interference value is not higher than the preset signal interference threshold, and the monitored enzyme reaction effective value is not lower than the preset enzyme reaction rate threshold and the residual interference degree value is not higher than the preset absorbance interference threshold, the execution is stopped, otherwise an alarm prompt is sent.

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