Experimental device for researching feeding and oviposition preference of warehouse insects to scattered storage objects

Abstract

An experimental device for studying feeding and oviposition preferences of warehouse insects to scattered storage objects comprises an experimental box and a selection platform arranged in the box, and the outer edge of the selection platform is attached to the inner wall of the experimental box so that a test space can be formed above the selection platform; the selection platform comprises a concave insect placing area and a sample distribution area surrounding the insect placing area, the sample distribution area is provided with a plurality of storage object sample grooves which are distributed at intervals in the circumferential direction of the insect placing area, the groove walls of the storage object sample grooves are inclined planes, and the groove opening area of the storage object sample grooves is larger than the groove bottom area; the upper surfaces of the insect placing area and the sample distribution area are provided with protrusions facilitating crawling of warehouse insects. An insect releasing cylinder capable of moving up and down penetrates through a box cover of the experiment box, the upper end of the insect releasing cylinder extends out of the box cover and is provided with a cylinder cover, and the lower end of the insect releasing cylinder is open and can abut against the insect containing area after moving downwards. According to the invention, the stored insect pests can be allowed to freely contact and select different foodstuffs in the experiment process, so that the feeding and oviposition preference of the stored insect pests can be more accurately researched.

Description

Technical Field

[0001] The invention relates to the field of storage pest research, in particular to an experimental device for studying the feeding and egg-laying preferences of warehouse insects on scattered storage objects. Background Art

[0002] The feeding and oviposition preferences of insects can directly reflect the suitability of hosts for the growth, development and population expansion of pests. By studying the feeding and oviposition preferences of stored product insects (i.e., warehouse insects), it is helpful to determine the degree to which different types of grains, beans and other stored products and their processed states are susceptible to pest infection, so as to formulate more scientific and effective stored product management and prevention strategies. However, the current devices targeting insect selection preferences are mainly aimed at agricultural pests. The hosts of these pests are usually growing plants, and they feed on fresh plant tissues such as leaves and rhizomes. The experimental devices are usually designed to simulate field environments and fix different plants to observe the pests' selection preferences for different plant species or parts.

[0003] The food of stored food pests is often highly scattered grains or grain powder, and most devices used to study the preferences of agricultural pests are obviously unable to fix granular foods such as grains and beans. Highly scattered foods may be unevenly distributed during the experiment due to inappropriate device structure, which in turn affects the behavior of insects. Currently, there are relatively few devices for stored food pests, and there are only some devices for studying olfactory selection behavior, that is, the test insects make choices based on the smell of the food, and do not touch the food during the selection process, which can only reflect the insects' preference for the smell of the food.

[0004] For example, the Chinese invention patent with patent number CN202111008506.3 discloses a device for observing the host selection of stored grain insects, such as Figure 7 As shown, different odor sources are introduced into the device through two arms, and the test insect is released from the main arm. When it crawls into a certain arm and crawls a certain distance, it is considered to have made a choice.

[0005] Another example is the Chinese invention patent with patent number CN202210685259.9, which discloses a test device for comparing the preference of crawling warehouse insects for multiple food selections. Figure 5 , 6 As shown, after the test insect is placed in the center of the device, it is allowed to choose food from different food cups around the circular platform. When it approaches its favorite food, it will fall into the food cup, and it is considered to have made a preferential choice.

[0006] Before feeding and laying eggs, insects need to evaluate the host through multiple senses such as touch and taste in addition to smell. The above two devices in the prior art both reflect the olfactory preferences of pests for different foods or odor sources. However, the test insects do not come into contact with the foods during the selection process, so the feeding and egg-laying preferences of the insects cannot be tested. Summary of the invention

[0007] The present invention aims to provide an experimental device for studying the feeding and oviposition preferences of warehouse pests on scattered stored products, allowing the stored product pests to freely contact and select different foods during the experiment, so as to more accurately study their feeding and oviposition preferences.

[0008] In order to solve the above technical problems, the specific scheme adopted by the present invention is: an experimental device for studying the feeding and egg-laying preferences of warehouse beetles for scattered stored objects, comprising an experimental box and a selection platform arranged in the box, the outer edge of the selection platform is fitted with the inner wall of the experimental box to form a test space above the selection platform; the selection platform comprises a concave insect placement area and a sample distribution area surrounding the insect placement area, the sample distribution area is provided with a plurality of storage sample grooves distributed circumferentially around the insect placement area, the groove walls of the storage sample grooves are inclined, and the groove area of ​​the storage sample grooves is larger than the groove bottom area; the upper surfaces of the insect placement area and the sample distribution area are both provided with protrusions for facilitating the crawling of warehouse beetles; a worm releasing cylinder that can move up and down is penetrated on the box cover of the experimental box, the upper end of the worm releasing cylinder extends out of the box cover and is provided with a cylinder cover, the lower end of the worm releasing cylinder is open, and the lower end can be moved down and abut against the insect placement area.

[0009] As a further optimization of the above technical solution, an annular raised structure is formed between the insect placement area and the sample distribution area. Slopes are provided on the side of the raised structure facing the insect placement area and on the side facing the storage sample slot. The upper surface of the raised structure is provided with raised projections for facilitating the crawling of warehouse insects.

[0010] As a further optimization of the above technical solution, the notch of the storage sample slot is fan-shaped.

[0011] As a further optimization of the above technical solution, the plane of the platform is selected to be concave to form the storage sample groove.

[0012] As a further optimization of the above technical solution, a gravity valve is provided at the bottom of the storage sample tank.

[0013] As a further optimization of the above technical solution, the outer wall of the insect releasing tube is provided with an external thread, the box cover is provided with a mounting hole, and the hole wall of the mounting hole is provided with an internal thread matching the external thread.

[0014] As a further optimization of the above technical solution, the inner wall of the experimental box is provided with a supporting portion for supporting the selection platform.

[0015] As a further optimization of the above technical solution, the support portion is a boss or a bump arranged along the circumference of the inner wall of the box.

[0016] As a further optimization of the above technical solution, an outer edge of the platform is selected to be provided with an enclosure, the outer wall of the enclosure is connected to the inner side of the side wall of the box body, and the upper edge of the enclosure is connected to the inner side of the top of the box cover.

[0017] As a further optimization of the above technical solution, a rubber ring is provided at the joint between the box body and the box cover of the experimental box, and a clamp lock for clamping the box body and the box cover is provided on the outside of the experimental box.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The surface of the selection platform of the present invention is provided with a protrusion, and the insect placement area is set to be concave. On the one hand, when the lower end of the insect releasing cylinder abuts against the insect placement area, an acute angle is formed between the side wall of the insect placement area and the insect releasing cylinder, which prevents the test insects from escaping from the insect releasing cylinder to a certain extent. On the other hand, since the insect releasing cylinder itself is relatively long, there may be position deviations during the up and down movement. The insect placement area is set to be concave, so that the lower end of the insect releasing cylinder can be accurately fixed in the insect placement area of ​​the selection platform.

[0020] The protrusions designed on the surface of the selection platform and the sufficiently high test space in the present invention are helpful for the test insects to crawl and fly. In addition, the cross-sectional area of ​​the storage sample slot gradually decreases along its depth direction, and the side wall of the storage sample slot is an inclined surface for the test insects to crawl, so that the test insects can freely enter and exit between different samples. The fan-shaped cross-sectional design makes full use of the selection platform space and increases the contact area between the storage and the test insects in each storage sample slot.

[0021] The present invention adopts a threaded insect release cylinder and a rotating hand wheel, and can control the release of the test insects by accurately controlling the lifting and lowering of the insect release cylinder, which can not only ensure that the test insects start from the center of the platform, but also control the release timing of the test insects, and release them after they adapt to the test environment. This design not only avoids the randomness of the test insects during the release process, ensuring that each experiment can be carried out from the same starting point, but also provides the test insects with time and space to adapt to the test environment, reducing the impact of environmental changes or fright on the behavior of the test insects, and significantly improving the controllability and repeatability of the experiment.

[0022] The present invention designs a storage sample slot with a cone structure on the selection platform, and is equipped with a self-flow valve at the bottom. This innovative design can not only better adapt to and utilize the characteristics of scattered storage materials such as grains, beans and powdered processed products, so that the samples are well fixed in the sample slot, but also can flow out smoothly in a controllable manner. In addition, since all sample slots have the same structure and equal distance settings, when equal volume samples are placed, the stacking height and surface area of ​​each sample remain consistent. Therefore, no matter how the nature of the sample is, when the test worm starts from the center of the platform, the distance from each sample is the same, ensuring the accuracy and repeatability of the experiment. One of the keys to this design is the combined use of the cone structure and the self-flow valve, which not only ensures that the sample is fixed and does not scatter, but also unifies the distance between the test worm and each sample, thereby eliminating the influence of distance differences on the test worm's selection behavior.

[0023] The present invention adopts a pressure-sealed anti-escape design by arranging a circular rubber sealing ring between the box cover and the box body, and combining the close connection between the stainless steel locking clip and the front hook. This design forms a firm seal between the box cover and the box body, and between the box cover and the selection platform enclosure through the pressure applied by the locking clip, which can effectively prevent warehouse bugs from escaping from the joints of different structures. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a side cross-sectional schematic diagram of the experimental device;

[0025] Figure 2 It is a side view schematic diagram of the experimental device;

[0026] Figure 3 A top view schematic diagram of the selected platform;

[0027] Figure 4 It is a top view schematic diagram of the experimental device;

[0028] Figure 5 It is a side view schematic diagram of a test device for comparing the preference for multiple food selection of crawling warehouse insects in the prior art;

[0029] Figure 6 It is a top view schematic diagram of a crawling warehouse beetle multi-food selection preference comparison test device in the prior art;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of a host selection observation device for stored-grain insects in the prior art;

[0031] Figure numerals: 1. box body, 2. self-flow valve, 3. storage sample slot, 4. rubber ring, 5. clamp lock, 501. front hook, 502. locking clamp, 6. insect release cylinder, 7. box cover, 8. mounting hole, 9. guide sleeve, 10. rotating hand wheel, 11. cylinder cover, 12. test insects, 13. storage, 14. selection platform, 1401. insect placement area, 1402. raised structure, 1403. sample distribution area, 1404. enclosure, 15. support part, 16. test space. DETAILED DESCRIPTION

[0032] The technical solution of the present invention is further elaborated in detail below in conjunction with specific embodiments. The parts of the present invention that are not described and disclosed in detail in the following embodiments should be understood as the prior art known or should be known to those skilled in the art.

[0033] like Figure 1-3 As shown, the present invention discloses an experimental device for studying the feeding and egg-laying preferences of warehouse beetles for scattered stored objects, including an experimental box, a selection platform 14 arranged in the experimental box, and an insect release tube 6 for conveying test insects 12 to the selection platform 14. The outer edge of the selection platform 14 is fitted with the inner wall of the experimental box to form a test space 16 above the selection platform 14 for the test insects 12 to fly. The upper surface of the selection platform 14 is provided with protrusions to facilitate the crawling of the test insects 12. The test insects 12 are warehouse beetles to be studied.

[0034] Specifically, the outer contour of the experimental box is cylindrical, and a cylindrical inner cavity is formed therein. The experimental box includes a box body 1 and a box cover 7, both of which are made of black non-light-transmitting materials, and the inner wall is made of a smooth material. The selection platform 14 is a circular transparent platform horizontally arranged in the experimental box, and the plane where the selection platform 14 is located is a horizontal plane.

[0035] The selection platform 14 includes a concave insect placement area 1401 and a sample distribution area 1403 surrounding the insect placement area 1401, and the insect placement area 1401 is circular and is distributed concentrically with the selection platform 14. A plurality of storage sample slots 3 are arranged around the insect placement area 1401 at intervals in the circumference. The edge of the selection platform 14 is provided with a fence 1404 with a smooth inner wall to prevent the test insect 12 from escaping. The outer wall of the fence 1404 is connected to the inner side of the side wall of the box cover 7, and the upper edge of the fence 1404 is connected to the inner side of the cover top of the box cover 7. The height of the fence 1404 is more than 10 cm, so the test space 16 has enough height for the test insect 12 to fly.

[0036] An annular raised structure 1402 is formed between the insect placing area 1401 and the sample distribution area 1403. The raised structure 1402 has slopes on the side facing the insect placing area 1401 and on the side facing the storage sample slot 3. The inclined surface of the raised structure 1402 facing the insect placing area 1401 is the side wall of the concave insect placing area 1401.

[0037] The slope of the raised structure 1402 on the side facing the insect placement area 1401 is relatively steep, and preferably, the acute angle formed between the raised surface and the horizontal plane is 45-75 degrees; the slope of the raised surface on the side facing the storage sample slot 3 is relatively gentle, and preferably, the acute angle formed between the raised surface and the horizontal plane is 15-30 degrees. In this experimental device, the insect placement area 1401 is set as a concave structure, and the angle between the side wall of the insect placement area 1401 and the side wall of the insect release tube 6 is an acute angle. Since the lower end of the insect release tube 6 can abut against the upper surface of the insect placement area 1401, and the upper surface of the insect placement area 1401 is provided with a raised projection, a gap is inevitably formed between the lower end of the insect release tube 6 and the upper surface of the insect placement area 1401. Setting the angle between the side wall of the insect placement area 1401 and the side wall of the insect release tube 6 to an acute angle can facilitate the release of the insect tube. After the lower end of the insect releasing cylinder 6 is separated from the insect placing area 1401, the test insect 12 crawls out from the insect placing area 1401. When the lower end of the insect releasing cylinder 6 abuts against the insect placing area 1401, the test insect 12 is prevented from escaping from the gap between the insect releasing cylinder 6 and the insect placing area 1401 to a certain extent. In addition, since the insect releasing cylinder 6 itself is relatively long, there may be position deviation during the up and down movement. The insect placing area 1401 is set to be concave, so that the lower end of the insect releasing cylinder 6 can be accurately fixed in the insect placing area 1401 of the selection platform 14.

[0038] In other embodiments of the present invention, the inclined surface of the protruding structure 1402 facing the insect placing area 1401 is connected to the side wall of the concave insect placing area 1401.

[0039] There are six storage sample slots 3 in this embodiment, and the slot wall is an inclined plane for the warehouse insect to crawl, and the inclined plane on the side facing the protruding structure 1402 is relatively gentle, and the acute angle formed between the inclined plane and the horizontal plane is 15-30 degrees, and the inclined plane on the side away from the protruding structure 1402 is relatively steep, and the acute angle formed between the inclined plane and the horizontal plane is 45-75 degrees, and the adjacent side walls between different storage sample slots 3 are also relatively gentle, and the acute angle between the inclined plane and the horizontal plane is also 15-60 degrees; the inclined plane of this angle can increase the resistance of the test insect 12 in the storage sample slot 3 to crawl to the outer edge of the selection platform 14, so that the test insect 12 can freely choose between different storage sample slots 3. The notch of the storage sample slot 3 is fan-shaped, the bottom of the slot is circular, and the notch area is larger than the bottom area of ​​the slot. The fan-shaped cross-section design makes full use of the selection platform space and increases the contact area between the storage and the test insect in each storage sample slot 3. It should be noted that the fan-shaped notch of the storage sample slot 3 in the present invention refers to a notch in the shape of a fan, including an inner arc close to the insect placement area 1401, an outer arc away from the insect placement area 1401, and two side edges connected to the inner arc and the outer arc. The arc length of the inner arc is smaller than that of the outer arc, and the diameter of the circular groove bottom is smaller than the arc length of the inner arc.

[0040] The storage sample slot 3 is a cone-like structure as a whole, and the cross-section gradually decreases from top to bottom. A circular self-flowing valve 2 is provided at the bottom of the slot. The self-flowing valve 2 is used to release the storage material 13 in the slot. The setting of the storage sample slot 3 can ensure that the distance from the insect placement area 1401 is the same when the volume of scattered samples is the same.

[0041] The storage sample slot 3 and the selection platform 14 are integrally formed, that is, the plane of the selection platform 14 is concave to form the storage sample slot 3 .

[0042] The present invention designs a storage sample slot 3 of a cone structure on the selection platform 14, and is equipped with a self-flowing valve 2 at the bottom, which can solve the problem that the scattered samples existing in the current insect selection behavior research device are difficult to effectively fix, and can not only better adapt to and utilize the characteristics of scattered storages such as grains, beans and powdered processed products, so that different storage samples can be well fixed in the sample slot, but also can flow out smoothly in a controllable manner. In addition, since all storage sample slots 3 have the same structure and equal distance settings, when equal volume samples are placed, the stacking height and surface area of ​​each sample remain consistent. Therefore, no matter how the nature of the sample is, when the test insect 12 starts from the center of the platform, the distance from each sample is the same, ensuring the accuracy and repeatability of the experiment. The combined use of the storage sample slot 3 of the cone structure and the self-flowing valve 2 not only ensures that the sample is fixed and not scattered, but also unifies the distance between the test insect 12 and each sample, thereby eliminating the influence of the distance difference on the selection behavior of the test insect 12, and also provides a convenient and efficient sample recovery method. Scattered food is usually difficult to remove from the groove, but the design of the gravity valve 2 can effectively solve this problem. Through the gravity valve 2, researchers can easily control the outflow of samples, so that scattered food samples can be smoothly discharged from the sample slot 3, thereby simplifying the sample recovery process after the experiment and improving the experimental operation efficiency.

[0043] The upper surface of the selection platform 14 (including the insect placement area 1401, the sample distribution area 1403 and the protrusion structure 1402) is provided with protrusions for the crawling of the warehouse insects. Since some warehouse insects live in grains or powder for a long time, their feet cannot adapt to a completely smooth surface and crawl. Therefore, granular protrusions are provided on the surface of the selection platform 14 to make the surface of the selection platform 14 a rough surface to avoid the influence of the smooth surface on the behavior of the insects.

[0044] The insect releasing cylinder 6 is passed through the box cover 7 of the experimental box and can move up and down. The upper end of the insect releasing cylinder 6 extends out of the box cover 7 and is provided with a cylinder cover 11. The lower end of the insect releasing cylinder 6 is open and can move downward and abut against the insect placing area 1401.

[0045] The box cover 7 is provided with a mounting hole 8 for mounting the insect release cylinder 6. The hole wall of the mounting hole 8 is provided with an internal thread. The box cover 7 and the insect release cylinder 6 are connected by threads to realize the up and down movement of the insect release cylinder 6.

[0046] The insect release cylinder 6 is a cylindrical non-light-transmitting structure as a whole, with the same inner diameter from top to bottom, a smooth inner wall, and an outer wall divided into three parts. The lower part is a smooth structure, the middle part is an external thread used in conjunction with the hole wall of the mounting hole 8, and the upper part has a circle of protruding rotating handwheel 10, and the surface has anti-slip lines, which is convenient for hand-held rotation operation. There is also a section of external thread matching the cylinder cover 11 above the rotating handwheel 10. The cylinder cover 11 is threadedly connected to the upper end of the insect release cylinder 6. The inner diameter of the cylinder cover 11 is consistent with the outer diameter of the insect release cylinder 6, and can be tightened with the upper end of the insect release cylinder 6 to achieve a sealed effect. The upper end of the cylinder cover 11 is embedded with a 180-mesh stainless steel mesh as a cover top, and the diameter of the mesh is about 3 / 4 of the diameter of the cylinder cover 11, which can ensure gas circulation and prevent the test insects 12 from escaping.

[0047] In order to improve the stability of the insect release cylinder 6, a guide sleeve 9 is fixedly arranged in the mounting hole 8 of the box cover 7, and an internal thread matching the external thread on the insect release cylinder 6 is provided on the inner wall of the guide sleeve 9, and the length of the guide sleeve 9 is 1 / 3-1 / 5 of the length of the insect release cylinder 6. In addition, a guide sleeve 9 having the same diameter as the mounting hole 8 can be arranged on the inner side and / or the outer side of the top of the box cover 7, and the guide sleeve 9 can be fixed on the top of the box cover 7.

[0048] The present invention adopts a threaded insect release cylinder 6 and a rotating hand wheel 10, and can accurately control the lifting and lowering of the insect release cylinder 6 to control the release of the test insect 12, which can not only ensure that the test insect 12 starts from the center of the platform, but also control the release timing of the test insect 12, and release it after it adapts to the test environment. This design not only avoids the randomness of the test insect 12 during the release process, ensuring that each experiment can be carried out from the same starting point, but also provides the test insect 12 with time and space to adapt to the test environment, reducing the impact of environmental changes or fright on the behavior of the test insect 12, and significantly improving the controllability and repeatability of the experiment.

[0049] The inner wall of the box body 1 is provided with a support portion 15 for supporting the selection platform 14, and the support portion 15 is a boss or a convex block provided along the circumference of the inner wall of the box body 1. The lower edge of the selection platform 14 is supported on the support portion 15, and the storage sample tank 3 is suspended in the box body 1, and a certain space is left between the self-flow valve 2 at the bottom of the storage sample tank 3 and the bottom of the box body 1.

[0050] The outside of the experimental box is provided with a clamp lock buckle 5 for clamping the box body 1 and the box cover 7. Four stainless steel front hooks 501 (such as Figure 3 , 4As shown), the front hook 501 and the locking clip 502 correspond to the position of the locking clip 502 on the box cover 7, and the front hook 501 and the locking clip 502 constitute the clamp lock buckle 5. The specific structure of the front hook 501 and the locking clip 502 is the prior art and will not be repeated here. The box body 1 and the box cover 7 of the experimental box are consistent in height, inner diameter and outer diameter, and a circular rubber ring 4 is provided at the joint of the box body 1 and the box cover 7 to ensure that after the locking clip 502 and the front hook 501 are buckled, the box body 1 and the box cover 7, and the box cover 7 and the upper edge of the platform enclosure 1404 are tightly connected, and the test insect 12 cannot escape, and the clamp lock buckle 5 and the rubber ring 4 cooperate to form a pressure-sealed anti-escape mechanism. At the same time, the inner walls of the box cover 7, the selection platform enclosure 1404, the insect release tube 6, etc. are all made of smooth materials, which further reduces the possibility of the test insect escaping.

[0051] Warehouse insects are usually small in size and have strong drilling ability, and are very easy to escape from the gaps of the device. In view of this characteristic, the present invention adopts a pressure-sealed anti-escape design, so that a firm seal is formed between the box cover 7 and the box body 1, and between the box cover 7 and the enclosure 1404 of the selection platform 14, which can effectively prevent warehouse insects from escaping from the connection between different boxes 1 and box covers 7. At the same time, the inner walls of the box cover 7, the enclosure 1404 of the selection platform 14, and the insect release tube 6 are all made of smooth materials, which further reduces the possibility of the test insect 12 escaping. In addition, the outer wall of the enclosure 1404 set on the selection platform 14 fits the inner wall of the box cover 7 and the box body 1, and the enclosure 1404 is located on the inner side of the connection between the box cover 7 and the box body 1, which can further seal the box cover 7 and the box body 1 through the enclosure 1404, further preventing the test insect 12 from escaping.

[0052] In the present invention, the granular protrusions designed on the surface of the selection platform 14 and the sufficiently high test space 16 facilitate the crawling and flying of the test insect 12. In addition, the longitudinal pyramidal structure between adjacent storage sample slots 13 forms a non-vertical slope, allowing the test insect 12 to freely enter and exit between different storage sample slots 13. The fan-shaped cross-section design fully utilizes the space of the selection platform 14 and increases the contact area between the sample in each storage sample slot 13 and the test insect 12. In contrast, the existing devices usually use the crawling distance of the test insect (such as Figure 6 ) or whether it falls into a vertical trap (such as Figure 4 , 5 ) to judge the selection result. These methods are based only on the sense of smell to judge the reaction of the test insect 12 to the odor emitted by the sample, and fail to achieve real contact with the sample, so they cannot effectively simulate the feeding and egg-laying behavior of the test insect 12. In addition, the existing devices cannot test flying warehouse insects. The present invention can meet the needs of crawling warehouse insects and flying warehouse insects at the same time by forming a relatively closed test space 16 above the selection platform 14, and has wide adaptability.

[0053] Insects usually need to evaluate the host through multiple senses such as touch and taste before feeding and laying eggs. In the prior art (such as patent CN202210685259.9), the test insect is judged completely by smell during the selection process. Once it falls into the food cup, it cannot climb out. It is considered that the selection is complete and the experiment ends. Therefore, it can only be judged by this device which kind of smell the insect prefers. After the test insect 12 falls, even if it is found that the hardness, shape, size, taste, etc. of the food are not suitable, it can only lay eggs and feed in the food cup, so this type of device is usually not used to measure the egg laying and feeding preferences of the test insect 12, mainly used to reflect the attraction of the smell of different food to the test insect 12. After the test insect 12 is put in, the selection can be completed in generally a few seconds or minutes, and the experimental time is short. The present invention ensures that the test insect 12 can freely and fully contact each sample by providing sufficient contact time and space. It usually needs to wait for several days to take out the test insect 12 after laying eggs. The experiment lasts for a long time, so as to more accurately reflect its natural behavior selection. This design not only improves the scientific nature of the experiment and the reliability of the results, but also fills the gap in the current lack of corresponding equipment when studying the feeding and egg-laying preference behaviors of warehouse beetles.

[0054] Next, the device of the present invention is used to carry out the following application experiments:

[0055] Experimental Example 1 (Study on the egg-laying preference of green bean weevils for 6 different beans)

[0056] Experimental device and sample preparation: This embodiment uses the experimental device of the present invention to study the oviposition preference of bean weevils for 6 different beans. The experimental device includes a box cover 7, a selection platform 14, a storage sample slot 13, an insect release tube 6 and a self-flow valve 2. The storage sample slot 13 on the selection platform 14 adopts a cone structure design, and a self-flow valve 2 is arranged at the bottom to ensure the fixation and controllable outflow of scattered bean samples. Six different varieties of beans were selected for the experiment. 50 ml of each bean sample was taken and poured into 6 storage sample slots 13 respectively. The samples in each storage sample slot 13 were leveled to make the stacking surface level and the height consistent, ensuring that the test insect 12 is at an equal distance from each sample when starting from the insect placement area 1401.

[0057] Device assembly and release of test insects 12: First, place the selection platform 14 loaded with 6 kinds of bean samples at the receiving position of the box body 1, then place the rubber ring 4 and the box cover 7 on the box body 1 in turn, align and lock the lock clip 502 and the front hook 501 of the stainless steel clip lock buckle 5 of the box cover 7 with the box body 1 to form a closed and dark test space 16. Next, insert the insect release cylinder 6 from the installation hole 8 of the box cover 7, and rotate the insect release cylinder 6 so that its bottom is in close contact with the insect placement area 1401 of the selection platform 14 to ensure that the test insects 12 can be accurately guided to the center of the platform. Subsequently, the entire device is placed in a constant temperature and humidity incubator required for the experiment for balance. After the temperature and humidity inside and outside the box are consistent, 30 mated female adults of the same period are released into the insect release cylinder 6 and the cylinder cover 11 is tightened. After they are fully adapted to the test environment, slowly rotate the hand wheel of the insect release cylinder 6 to make it rise, release the test insects 12 and start the experiment.

[0058] Experimental setup and result analysis: The timing started 12 hours after the test insects were released. After the insects had laid eggs freely in the device for 72 hours, the selection platform 14 was taken out and the gravity valve 2 was opened to discharge the sample into the culture dish. After all the adults were removed, the number of eggs on different beans was observed and recorded under a microscope. The egg-laying preference of the bean weevil for different beans was analyzed by counting the number of eggs laid by the bean weevil in the 6 sample slots.

[0059] Experimental Example 2 (Study on the feeding preference of corn weevils on three wheat varieties)

[0060] Experimental device and sample preparation: This embodiment uses the experimental device of the present invention to study the feeding preference of corn weevils for three different wheat varieties. The experimental device includes a box cover 7, a selection platform 14, a storage sample slot 13, an insect release tube 6 and a self-flowing valve 2. The storage sample slot 13 on the selection platform 14 adopts a cone structure design, and a self-flowing valve 2 is arranged at the bottom to ensure the fixation and controllable outflow of scattered wheat samples. Three different varieties of wheat were selected for the experiment. Two 40ml samples of each variety were taken and weighed, and the same variety was symmetrically poured into the storage sample slot 13. The samples in each storage sample slot 13 were leveled to make the stacking surface level and the height consistent, ensuring that the corn weevils are at an equal distance from each sample when starting from the insect placement area 1401.

[0061] Device assembly and release of test insects 12: First, place the selection platform 14 loaded with wheat samples at the receiving position of the box body 1, then place the rubber ring 4 and the box cover 7 on the box body 1 in turn, align the lock clip 502 and the front hook 501 of the stainless steel clip lock buckle 5 of the box cover 7 with the box body 1 and lock them, forming a closed and dark test space 16. Next, insert the insect release cylinder 6 from the installation hole 8 of the box cover 7, and rotate the insect release cylinder 6 so that its bottom is in close contact with the insect placement area 1401 of the selection platform 14, ensuring that the test insects 12 can be accurately guided to the center of the platform. Subsequently, the entire device is placed in a constant temperature and humidity incubator required for the experiment for balance. After the temperature and humidity inside and outside the box are consistent, 100 corn weevils are released into the insect release cylinder 6 and the cylinder cover 11 is tightened. After they are fully adapted to the test environment, the hand wheel of the insect release cylinder 6 is slowly rotated to make it rise, release the test insects 12 and start the experiment.

[0062] Experimental setup and result analysis: The timing started after the test insects were released 12 times. After the insects had been feeding freely in the device for 14 days, the selection platform 14 was taken out and the gravity valve 2 was opened to allow the sample to flow out into the culture dish. After all the adults were removed, the sample was weighed again. By statistically analyzing the weight loss and insect infestation rate of each variety of wheat, the feeding preference of corn weevils on different wheat varieties was analyzed and compared.

[0063] Experimental Example 3 (Study on the oviposition preference of rice borer for rice in different processing states)

[0064] Experimental device and sample preparation: This embodiment uses the experimental device of the present invention to study the oviposition preference of rice borer to 6 different rice processing states of hulled rice, brown rice, rice, rice flour, brown rice flour and rice bran. The experimental device includes a box cover 7, a selection platform 14, a storage sample slot 13, an insect release tube 6 and a self-flow valve 2. The storage sample slot 13 on the selection platform 14 adopts a cone structure design, and a self-flow valve 2 is set at the bottom to ensure the fixation and controllable outflow of scattered samples. 40ml of each sample is poured into the storage sample slot 13, and the samples in each storage sample slot 13 are leveled to make the stacking surface level and height consistent, ensuring that the rice borer is equal to each sample when starting from the insect placement area 1401.

[0065] Device assembly and release of test insects 12: First, place the selection platform 14 loaded with samples at the receiving position of the box body 1, then place the rubber ring 4 and the box cover 7 on the box body 1 in turn, align and lock the lock clip 502 and the front hook 501 of the stainless steel clip lock buckle 5 of the box cover 7 with the box body 1 to form a closed and dark test space 16. Next, insert the insect release cylinder 6 from the installation hole 8 of the box cover 7, and rotate the insect release cylinder 6 so that its bottom is in close contact with the insect placement area 1401 of the selection platform 14 to ensure that the test insects 12 can be accurately guided to the center of the platform. Subsequently, the entire device is placed in a constant temperature and humidity incubator required for the experiment for balance. After the temperature and humidity inside and outside the box are consistent, 50 adult rice borers are released into the insect release cylinder 6 and the cylinder cover 11 is tightened. After they are fully adapted to the test environment, slowly rotate the handwheel of the insect release cylinder 6 to make it rise, release the test insects 12 and start the experiment.

[0066] Experimental setup and result analysis: Start timing after releasing the test insect 12, and remove the selection platform 14 after it lays eggs freely in the device for one week. Since the eggs of the grain borer are small and often hidden between the gaps, it is difficult to directly observe and count the number, so the number of eggs laid is often indirectly reflected by observing the number of offspring emerging. After opening the sample tank self-flow valve 2 to allow the sample to drain into the culture dish, remove all adults and place them in different culture bottles for culture. After the offspring adults begin to emerge, record the number of offspring adults every day until no new adults emerge, count the total number of individuals emerging from different samples, and compare their egg-laying preferences on different samples.

[0067] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. An experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects, characterized in that: It comprises an experimental box and a selection platform (14) arranged in the box, wherein the outer edge of the selection platform (14) is in contact with the inner wall of the experimental box to form a test space above the selection platform (14); The selection platform (14) comprises a concave insect placement area (1401) and a sample distribution area (1403) surrounding the insect placement area (1401); the sample distribution area (1403) is provided with a plurality of storage sample grooves (3) distributed at intervals around the insect placement area (1401); the groove walls of the storage sample grooves (3) are inclined, and the groove opening area of ​​the storage sample grooves (3) is larger than the groove bottom area; the upper surfaces of the insect placement area (1401) and the sample distribution area (1403) are both provided with protrusions for facilitating crawling of the warehouse insects; An insect releasing cylinder (6) that can move up and down is provided on the box cover (7) of the experimental box. The upper end of the insect releasing cylinder (6) extends out of the box cover (7) and is provided with a cylinder cover (11). The lower end of the insect releasing cylinder (6) is open and can be moved downward to abut against the insect placing area (1401).

2. An experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: An annular raised structure (1402) is formed between the insect placement area (1401) and the sample distribution area (1403), and the raised structure (1402) is provided with slopes on the side facing the insect placement area (1401) and the side facing the storage sample slot (3), and the upper surface of the raised structure (1402) is provided with a protrusion that facilitates the crawling of warehouse insects.

3. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: The plane of the platform (14) is selected to be concave to form the storage sample slot (3).

4. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: The notch of the storage sample slot (3) is fan-shaped.

5. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: A self-flow valve (2) is arranged at the bottom of the storage sample tank (3).

6. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: The outer wall of the insect releasing cylinder (6) is provided with an external thread, the box cover (7) is provided with a mounting hole (8), and the hole wall of the mounting hole (8) is provided with an internal thread matching the external thread.

7. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: The inner wall of the box body (1) of the experimental box is provided with a supporting portion (16) for supporting the selection platform (14).

8. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 7, characterized in that: The support portion (16) is a boss or a bump arranged along the circumference of the inner wall of the box body (1).

9. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: The outer edge of the selection platform (14) is provided with a baffle (1404), the outer wall of the baffle (1404) is connected to the inner side of the side wall of the box body, and the upper edge of the baffle (1404) is connected to the inner side of the top of the box cover (7).

10. The experimental device for studying the feeding and egg-laying preferences of warehouse beetles on scattered stored objects according to claim 1, characterized in that: A rubber ring (4) is provided at the joint between the box body (1) and the box cover (7) of the experimental box, and a clamping lock (5) for clamping the box body (1) and the box cover (7) is provided outside the experimental box.

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