Anti-aging composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of anti-aging products, and particularly relates to an anti-aging composition as well as a preparation method and application thereof. The anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5%-1% of xanthophyll, 0.1%-0.3% of pyrroloquinoline quinone, 0.01%-0.02% of sodium propyl paraben, 0.5%-1% of an emulsifier and the balance of water. The emulsifier is a non-ionic emulsifier. The anti-aging composition prepared by the invention has definite components, is free of allergic reactions such as skin redness, rash and itching through tests of multiple testees, and is milder and significantly reduced in sensitization rate compared with products in the prior art.

Description

Technical Field

[0001] The present invention belongs to the technical field of anti-aging products, and specifically relates to an anti-aging composition and a preparation method and application thereof. Background Art

[0002] Pyrroloquinoline quinone (PQQ), also known as pyrroloquinoline quinone, is an aromatic tricyclic o-quinone compound. It is widely found in microorganisms, plants, animals and humans. Pyrroloquinoline quinone has strong antioxidant ability. Studies have shown that pyrroloquinoline quinone can also improve the body's resistance to disease by enhancing immune function, thereby helping to maintain the ovaries and achieve anti-aging effects. There are many studies on the anti-aging effect of pyrroloquinoline quinone in the prior art, such as the literature "Wang Jing, Shi Zhinan, Gu Li, et al. Inhibitory effect of pyrroloquinoline quinone on UVB-induced premature senescence of human skin fibroblasts [J]. Journal of Nantong University: Medical Edition, 2023, 43(3): 224-229, and Chinese patents CN114939153B, CN110025559B, etc.

[0003] Lutein is a fat-soluble vitamin with the following main functions: protecting eyesight, coloring and anti-oxidation. There are many studies on the anti-aging effect of lutein in the prior art, such as Chinese patents CN118384084A and CN112263529A.

[0004] Although there are many applications of pyrroloquinoline quinone and lutein in anti-aging in the prior art, they need to be compounded with more types of substances to form a composite in order to achieve a good anti-aging effect. For example, the formulas of Chinese patents CN114939153B, CN110025559B, CN118384084A, and CN112263529A all contain plant or animal extracts. In addition to the target active ingredients such as lutein, the extracts may also contain other unknown compounds. For people with allergies, products with unclear ingredients are prone to allergies. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides an anti-aging composition and its preparation method and application. The anti-aging composition has clear ingredients and has been tested by multiple subjects, and has no allergic reactions such as skin redness, rash, and itching. Compared with the products of the prior art, it is milder and has a significantly lower sensitization rate.

[0006] The first object of the present invention is to provide an anti-aging composition, which is mixed with the following raw materials in percentage by mass: 0.5% to 1% lutein, 0.1% to 0.3% pyrroloquinoline quinone, 0.01% to 0.02% sodium propylparaben, 0.5% to 1% emulsifier, and water to make up to 100%; the emulsifier is a non-ionic emulsifier.

[0007] Among them, lutein and pyrroloquinoline quinone play the main antioxidant role, can remove free radicals, inhibit the growth of fibroblasts, and delay skin aging. Therefore, the amount of lutein accounts for 0.5% to 1% of the total mass of the composition, and the amount of pyrroloquinoline quinone accounts for 0.1% to 0.3% of the total mass of the composition. Sodium propylparaben is used in low doses, accounting for only 0.01% to 0.02% of the amount of the composition. On the one hand, sodium propylparaben has the function of a stabilizer, which is used to stably disperse lutein and pyrroloquinoline quinone in water. On the other hand, sodium propylparaben is used to enhance the anti-aging effect of lutein and pyrroloquinoline quinone and enhance the inhibitory effect on fibroblasts. Emulsifiers provide emulsification and moisturizing effects, increase the contact between the composition and the skin, and help lutein and pyrroloquinoline quinone disperse in water. Non-ionic emulsifiers have mild chemical properties and basically do not cause allergic reactions. Water used is water that meets the requirements of cosmetics or water that meets the requirements of food.

[0008] The composition provided by the present invention involves a total of five ingredients, namely, lutein, pyrroloquinoline quinone, sodium propylparaben, an emulsifier and water. The ingredients are simple and clear. Compared with the anti-aging products in the prior art, the composition is milder and has a significantly lower sensitization rate.

[0009] Preferably, the anti-aging composition is made by mixing the following raw materials in percentage by mass: 0.7% lutein, 0.2% pyrroloquinoline quinone, 0.015% sodium propylparaben, 0.8% emulsifier, and 100% water. The composition of this formula has the most obvious effect of removing free radicals and the best effect of inhibiting the growth of fibroblasts.

[0010] Preferably, the nonionic emulsifier is polyoxyethylene-polyoxypropylene copolymer, polysorbate and its analogs, glyceryl stearate and its analogs, or sorbitan monostearate and its analogs.

[0011] More preferably, the nonionic emulsifier is glyceryl stearate or Pluronic F68. These are common emulsifiers that can be used on human skin.

[0012] It should be noted that the anti-aging composition provided by the present invention can be applied to skin anti-aging, and can also be used in cell anti-aging experiments or animal anti-aging experiments. When applied to cell anti-aging experiments or animal anti-aging experiments, without departing from the inventive concept of the present invention, the non-ionic emulsifier that can be selected by a person skilled in the art is polyoxyethylene-polyoxypropylene copolymer, polysorbate and its analogs, glycerol stearate and its analogs, or sorbitan monostearate and its analogs. More specifically, for example, the emulsifier is poloxamer, etc.

[0013] The second object of the present invention is to provide a method for preparing an anti-aging composition, comprising:

[0014] Prepare various raw materials according to mass percentage;

[0015] Firstly, sodium propylparaben is mixed with water, then an emulsifier is added and mixed, and finally lutein and pyrroloquinoline quinone are added and mixed evenly to obtain an anti-aging composition.

[0016] The best preparation method provided by the present invention is determined according to the properties of each raw material. Sodium propylparaben is easily soluble in water, so sodium propylparaben is first dispersed in water, and then an emulsifier is added to change the properties of water so that lutein and pyrroloquinoline quinone are dispersed. Mixing the raw materials in the above order has a better mixing effect and saves more mixing operation time than directly mixing all the raw materials.

[0017] Preferably, the raw materials are mixed by homogenizing means so that the dispersion of the raw materials will be more uniform.

[0018] The third object of the present invention is to provide an anti-aging composition for use in preparing anti-aging products.

[0019] Preferably, the anti-aging product has the purpose of removing free radicals and delaying skin aging.

[0020] Preferably, the anti-aging product is an anti-aging cosmetic or an anti-aging cell aging preparation.

[0021] For example, the anti-aging cosmetic is a milk, cream, gel or mask containing the anti-aging composition.

[0022] Lotion, cream, gel or mask are the corresponding auxiliary materials added to the anti-aging composition. Note that the ingredients of the auxiliary materials should be clear, and after the sensitization test, the sensitization rate of the subjects should be less than one in ten thousand.

[0023] For example, excipients suitable for milk include emulsifiers.

[0024] Suitable auxiliary materials for creams include one or more of oils, waxes and emulsifiers.

[0025] Suitable excipients for gels include gel formers, such as carbomer gel.

[0026] Suitable auxiliary materials for facial masks include one or more of non-woven fabrics, emulsifiers and gel formers.

[0027] For example, the anti-aging cell aging preparation is used to inhibit the proliferation of fibroblasts. Skin fibroblasts are sensitive to ultraviolet rays. Skin fibroblasts may overproliferate and synthesize collagen after being irradiated with ultraviolet rays, thereby causing inflammatory reactions in the skin. The anti-aging composition provided by the present invention can inhibit the proliferation of fibroblasts, and has a good alleviating effect on the excessive proliferation of fibroblasts caused by ultraviolet rays and other reasons, thereby improving problems such as skin inflammation.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention has developed a composition with lutein, pyrroloquinoline quinone, sodium propylparaben, emulsifier and water as components from multiple perspectives such as scavenging free radicals and inhibiting fibroblast proliferation. The composition is clear and basically non-allergenic. Compared with the products in the prior art, it is milder and has a significantly lower allergenic rate. The composition of the present invention also has a significant anti-aging effect.

[0030] The present invention also provides a method for preparing an anti-aging composition, so that lutein and pyrroloquinoline quinone have good dispersibility.

[0031] In addition, since lutein has the effect of protecting eyesight, after the composition of the present invention is applied to the skin at the corner of the eye, the lutein penetrates into the eye through the skin, improves the response of the visual system, and repairs damage. Therefore, the composition provided by the present invention can not only resist skin aging, reduce crow's feet, wrinkles, etc., but also has the advantage of improving eyesight. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a diagram showing the anti-aging effects of the skin at the corners of the eyes of volunteers of the present invention.

[0033] Among them, A is a picture before applying the composition of Example 1 of the present invention, and B is a picture before applying the composition of Example 1 of the present invention. DETAILED DESCRIPTION

[0034] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0035] Although there are many applications of pyrroloquinoline quinone and lutein in anti-aging in the existing technology, they need to be compounded with more types of substances to form compositions in order to achieve good anti-aging effects. For example, many existing formulas contain plant or animal extracts. In addition to the target active ingredients such as lutein, the extracts may also contain other unknown compounds. For people with allergies, products with unclear ingredients are prone to allergies.

[0036] Based on the above reasons, the present invention provides an anti-aging composition, which is mixed with the following raw materials in percentage by mass: 0.5% to 1% lutein, 0.1% to 0.3% pyrroloquinoline quinone, 0.01% to 0.02% sodium propylparaben, 0.5% to 1% emulsifier, and 100% water; the emulsifier is a non-ionic emulsifier. The composition provided by the present invention achieves both anti-aging and non-allergenic effects. There is no allergic reaction of skin redness, rash, and itching.

[0037] Based on the same inventive concept, the present invention also provides a method for preparing an anti-aging composition, comprising: preparing various raw materials according to mass percentage; first mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly, to obtain an anti-aging composition. The composition prepared by the method can directly act on objects such as skin, cells, and animals to achieve an anti-aging effect.

[0038] Based on the same inventive concept, the present invention also provides an application of an anti-aging composition in the preparation of an anti-aging product. The anti-aging product has the purpose of removing free radicals and delaying skin aging, and can also inhibit the proliferation of fibroblasts.

[0039] Some specific embodiments and corresponding experimental results are introduced below.

[0040] It should be noted that in the description of the present invention, unless otherwise specified, all reagents used are commercially available, and the methods of "mixing" and "homogenization" used are conventional techniques in the art. Pyrroloquinoline quinone CAS No. 72909-34-3, sodium propylparaben CAS No. 35285-69-9. Glyceryl stearate is specifically glyceryl monostearate, CAS No. 123-94-4, purchased from Guangzhou Huayu Biotechnology Co., Ltd., with an active ingredient content of 99%. Pluronic F68 is poloxamer 188 purchased from Shaanxi Zhengyi Pharmaceutical Excipients Co., Ltd.

[0041] In the description of the present invention, free radical scavenging experiments, fibroblast proliferation inhibition experiments, skin sensitivity experiments and allergy testing methods of subjects are all performed with reference to the prior art.

[0042] Example 1

[0043] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.1% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0044] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0045] Example 2

[0046] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.6% of lutein, 0.1% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0047] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0048] Example 3

[0049] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.7% lutein, 0.1% pyrroloquinoline quinone, 0.01% sodium propylparaben, 0.5% emulsifier, and 100% water; the emulsifier is glyceryl stearate.

[0050] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0051] Example 4

[0052] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.8% lutein, 0.1% pyrroloquinoline quinone, 0.01% sodium propylparaben, 0.5% emulsifier, and 100% water; the emulsifier is glyceryl stearate.

[0053] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0054] Example 5

[0055] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.9% lutein, 0.1% pyrroloquinoline quinone, 0.01% sodium propylparaben, 0.5% emulsifier, and 100% water; the emulsifier is glyceryl stearate.

[0056] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0057] Example 6

[0058] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 1% lutein, 0.1% pyrroloquinoline quinone, 0.01% sodium propylparaben, 0.5% emulsifier, and 100% water; the emulsifier is glyceryl stearate.

[0059] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0060] Examples 1 to 6 are several examples of single factor changes in lutein, used to explore the influence of lutein content on the anti-aging effect.

[0061] Example 7

[0062] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.2% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0063] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0064] Example 8

[0065] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.3% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0066] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0067] Example 1, Example 7 and Example 8 are several examples for single factor changes of pyrroloquinoline quinone, which are used to explore the influence of pyrroloquinoline quinone content on the anti-aging effect.

[0068] Example 9

[0069] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.1% of pyrroloquinoline quinone, 0.015% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0070] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0071] Example 10

[0072] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.1% of pyrroloquinoline quinone, 0.02% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0073] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0074] Example 1, Example 9 and Example 10 are several examples of single factor changes of sodium propylparaben, which are used to explore the influence of sodium propylparaben content on the anti-aging effect.

[0075] Embodiment 11

[0076] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.1% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is Pluronic F68.

[0077] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0078] Example 1 and Example 11 are several examples for different emulsifiers, which are used to explore the influence of different emulsifiers on the anti-aging effect.

[0079] Control group 1

[0080] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of an emulsifier and 100% of water; the emulsifier is glyceryl stearate.

[0081] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; and uniformly mixing the emulsifier and water by homogenizing means to obtain the anti-aging composition.

[0082] Control group 1 is only a combination of emulsifier and water, which is used to explore the anti-aging effect when only emulsifier is used.

[0083] Control group 2

[0084] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% lutein, 0.5% emulsifier, and 100% water; the emulsifier is glyceryl stearate.

[0085] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly adding an emulsifier into water, mixing, and finally adding lutein, mixing evenly by homogenizing means, to obtain the anti-aging composition.

[0086] The control group 2 had more lutein than the control group 1. By comparing the control group 1 and the control group 2, we can conclude that lutein has an anti-aging effect.

[0087] Control group 3

[0088] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.1% of pyrroloquinoline quinone, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0089] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly adding water to an emulsifier, mixing, and finally adding pyrroloquinoline quinone, mixing evenly by homogenizing means, and obtaining the anti-aging composition.

[0090] Compared with control group 1, control group 3 has increased pyrroloquinoline quinone. By comparing control group 1 and control group 3, the anti-aging effect of pyrroloquinoline quinone can be concluded.

[0091] Control group 4

[0092] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.01% of sodium propylparaben, 0.5% of an emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0093] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, and mixing them evenly by homogenizing means to obtain the anti-aging composition.

[0094] Compared with control group 1, control group 4 added sodium propylparaben. By comparing control group 1 and control group 4, the anti-aging effect of sodium propylparaben can be concluded.

[0095] Control group 5

[0096] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.1% of pyrroloquinoline quinone, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0097] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly adding water to an emulsifier, mixing, and finally adding lutein and pyrroloquinoline quinone, mixing evenly by homogenizing means, and obtaining the anti-aging composition.

[0098] Compared with the control group 1, the control group 5 increased the combination of lutein and pyrroloquinoline quinone. By comparing the control groups 1, 2, 3 and 5, the anti-aging effect of the combination of lutein and pyrroloquinoline quinone can be obtained.

[0099] Control group 6

[0100] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.5% of lutein, 0.01% of sodium propylparaben, 0.5% of emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0101] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding lutein, mixing evenly by homogenizing means to obtain the anti-aging composition.

[0102] Compared with the control group 1, the control group 6 added a combination of lutein and sodium propylparaben. By comparing the control groups 1, 2, 4 and 6, the anti-aging effect of the combination of lutein and sodium propylparaben can be concluded.

[0103] Control group 7

[0104] An anti-aging composition is prepared by mixing the following raw materials in percentage by mass: 0.1% of pyrroloquinoline quinone, 0.01% of sodium propylparaben, 0.5% of an emulsifier, and 100% of water; the emulsifier is glyceryl stearate.

[0105] The preparation method of the anti-aging composition comprises: preparing various raw materials according to mass percentage; firstly mixing sodium propylparaben with water, then adding an emulsifier, mixing, and finally adding pyrroloquinoline quinone, mixing evenly by homogenizing means, and obtaining the anti-aging composition.

[0106] Compared with control group 1, control group 7 added a combination of pyrroloquinoline quinone and sodium propylparaben. By comparing control group 1, control group 3, control group 4 and control group 7, it can be concluded that the anti-aging effect of the combination of pyrroloquinoline quinone and sodium propylparaben.

[0107] Experiment 1: Free radical scavenging experiment.

[0108] The specific method of the free radical scavenging experiment refers to the prior art. The present invention does not innovate the specific experimental method, and the method described in CN114939153B is referred to. The test method of each experimental group and other operating conditions such as "mixing" are kept consistent except for the above-mentioned clear formula and preparation method, so as to eliminate the interference of human factors on the results.

[0109] IC 50 is the sample concentration at which the free radical scavenging efficiency reaches 50%, IC 50 The smaller the value, the stronger the ability to scavenge free radicals. Data processing: Three parallel experiments were performed for each experimental group, and the average value was taken. The results are shown in Table 1. From the results in Table 1, it can be seen that Examples 1 to 11 have the best free radical scavenging effect, followed by Control Groups 5 to 7, and finally Control Group 4, Control Group 3 and Control Group 1. By comparing the data of each control group and each embodiment, it can be seen that lutein, pyrroloquinoline quinone and sodium propylparaben all have the effect of scavenging free radicals.

[0110] Table 1 Free radical scavenging experimental results

[0111]

[0112]

[0113] Experiment 2: Fibroblast proliferation inhibition experiment.

[0114] The experimental method includes the steps of resuscitation, culture, passage, drug treatment, etc. of human skin fibroblasts HSF, and is carried out with reference to the prior art CN115028683B.

[0115] Among them, the drug treatment settings are as follows:

[0116] Blank control group: An equal amount of sterile water was used to replace the drug. The blank control group was used to calculate IC 50 .

[0117] Examples 1 to 11 and control groups 1 to 7 were treated with corresponding compositions, respectively. Each drug was set at 1.0 μM, 5.0 μM, 10.0 μM, 25.0 μM, 50.0 μM, 100.0 μM, 250.0 μM, and 500.0 μM.

[0118] Fibroblast proliferation inhibitor IC 50 , that is, the sample concentration when the inhibition rate of human skin fibroblast HSF reaches 50%, IC 50 The smaller the value, the stronger the ability to inhibit fibroblast proliferation. The results are shown in Table 2. From Table 2, it can be seen that lutein, pyrroloquinoline quinone and sodium propylparaben all have the effect of inhibiting the growth of human skin fibroblasts HSF, among which lutein and pyrroloquinoline quinone have more significant inhibitory effects.

[0119] Table 2 Results of fibroblast proliferation inhibition experiment

[0120]

[0121]

[0122] Experimental results 3. Allergy test.

[0123] Each experimental group was tested on 50 volunteers. The composition of each experimental group was evenly applied to the corresponding skin position.

[0124] Sensitization rate = 100% × total number of allergic people / total number of subjects.

[0125] The test results are shown in Table 3. Figure 1 This is the anti-aging effect picture of the skin at the corner of one of the volunteers. There was no allergic reaction and the anti-aging effect was obvious.

[0126] Table 3 Sensitization rate

[0127]

[0128]

[0129] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes a preferred embodiment. Although the preferred embodiments of the present invention have been described, once those skilled in the art know the basic inventive concept, they can make other changes and modifications to these embodiments, and these changes and modifications all fall within the scope of the present invention.

[0130] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the equivalent technology of the present invention, the present invention is also intended to include these modifications and variations.

Claims

1. An anti-aging composition, characterized in that: The invention is prepared by mixing the following raw materials in percentage by mass: 0.5% to 1% lutein, 0.1% to 0.3% pyrroloquinoline quinone, 0.01% to 0.02% sodium propylparaben, 0.5% to 1% emulsifier, and water to make up to 100%; The emulsifier is a nonionic emulsifier.

2. The anti-aging composition according to claim 1, characterized in that The invention is prepared by mixing the following raw materials in percentage by mass: 0.7% of lutein, 0.2% of pyrroloquinoline quinone, 0.015% of sodium propylparaben, 0.8% of emulsifier and 100% of water.

3. The anti-aging composition according to claim 1, characterized in that The nonionic emulsifier is polyoxyethylene-polyoxypropylene copolymer, polysorbate and its analogs, glyceryl stearate and its analogs, or sorbitan monostearate and its analogs.

4. The anti-aging composition according to claim 3, characterized in that: The polyoxyethylene-polyoxypropylene copolymer is Pluronic F68.

5. The method for preparing the anti-aging composition according to claim 1, characterized in that: include: Prepare various raw materials according to mass percentage; Firstly, sodium propylparaben is mixed with water, then an emulsifier is added and mixed, and finally lutein and pyrroloquinoline quinone are added and mixed evenly to obtain an anti-aging composition.

6. Use of the anti-aging composition according to claim 1 in the preparation of anti-aging products.

7. Use of the anti-aging composition according to claim 6 in the preparation of anti-aging products, characterized in that: The anti-aging product has the purpose of removing free radicals and delaying skin aging.

8. Use of the anti-aging composition according to claim 6 in the preparation of anti-aging products, characterized in that: Anti-aging products are anti-aging cosmetics or anti-aging cell aging preparations.

9. Use of the anti-aging composition according to claim 8 in the preparation of anti-aging products, characterized in that: The anti-aging cosmetic is a milk, cream, gel or mask containing the anti-aging composition.

10. Use of the anti-aging composition according to claim 8 in the preparation of an anti-aging cell aging preparation, characterized in that: The anti-aging cell aging preparation is used to inhibit the proliferation of fibroblasts.

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