Traditional Chinese medicine composition, preparation method and application of traditional Chinese medicine composition in hair loss preventing, dandruff removing and itching relieving shampoo

Abstract

The invention belongs to the technical field of traditional Chinese medicines, mainly relates to a preparation method of a traditional Chinese medicine composition, and particularly relates to a traditional Chinese medicine composition, a preparation method and application of the traditional Chinese medicine composition in hair loss preventing, dandruff removing and itching relieving shampoo. The traditional Chinese medicine composition is obtained by fermenting a traditional Chinese medicine mixture. The traditional Chinese medicine mixture comprises the following components in parts by weight: 40-60 parts of polygonum multiflorum, 15-25 parts of ginseng, 25-35 parts of poria cocos, 10-20 parts of mint and 50-60 parts of egg white; in the method, a compound strain is selected for fermentation, and the compound strain comprises lactobacillus acidophilus CICC-6082 and lactobacillus crispatus CICC-25191. The traditional Chinese medicine composition has the efficacy of preventing alopecia and also has the efficacy of removing dandruff, relieving itching and resisting allergy.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and mainly relates to a preparation method of a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition, a preparation method and application thereof in anti-hair loss, anti-dandruff and anti-itching shampoo. Background Art

[0002] As people's health awareness improves, consumers are increasingly inclined to choose natural and non-irritating washing and care products. Chinese herbal shampoo meets this market demand with its unique natural ingredients and mild washing and care effects. Although traditional chemical shampoos meet the needs of cleaning and dandruff removal to a certain extent, the chemical ingredients in them may cause damage to the scalp and hair, such as silicone oil clogging hair follicles and sodium laurel sulfate inhibiting hair growth. Chinese herbal shampoo uses pure natural Chinese herbal plant ingredients, which are mild and non-irritating and can reduce damage to the scalp and hair. Compared with chemical shampoos, Chinese herbal shampoo is safer and healthier to use. Chinese herbal ingredients not only have a cleaning effect, but also have multiple functions such as nourishing, repairing, preventing hair loss, and relieving itching. Chinese herbal shampoo has broad application prospects. In the future, with the continuous advancement of science and technology and the in-depth research of Chinese medicine, Chinese herbal shampoo will pay more attention to the development of personalization, customization and efficiency to meet the needs of different consumers. Summary of the invention

[0003] In view of the above problems, the present invention provides a Chinese medicine composition for preventing hair loss, removing dandruff and relieving itching, which is applied to the field of shampoo. The Chinese medicine composition of the present invention is obtained by fermenting a Chinese medicine mixture; the Chinese medicine mixture includes 40-60 parts of Polygonum multiflorum, 15-25 parts of ginseng, 25-35 parts of Poria cocos, 10-20 parts of mint and 50-60 parts of egg white by weight; the fermentation uses a composite strain fermentation, and the composite strain includes Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191. The Chinese medicine composition has the effect of preventing hair loss, and also has the effects of removing dandruff, relieving itching and anti-allergy.

[0004] In a first aspect, the present invention provides a method for preparing a Chinese medicine composition, comprising the following steps:

[0005] S1. Raw material pretreatment: weigh 40-60g of Polygonum multiflorum, 15-25g of ginseng, 25-35g of Poria cocos, 10-20g of mint, and 50-60g of egg white; wash the Polygonum multiflorum, ginseng, Poria cocos, and mint, dry them, and crush them; the purpose of washing and drying is to remove impurities and moisture;

[0006] S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from the China Industrial Microbiological Culture Collection. Both were inoculated in MRS agar medium and cultured at 37°C for 20-24 hours to activate the strains. Subsequently, they were transferred to 500-1000mL MRS liquid medium shake flasks and fermented for another 8 hours. As a result, the concentrations of the two bacterial solutions reached 2.0-2.2×10 8 cfu / mL. Both bacterial cultures were collected and stored in bacterial culture containers;

[0007] S3, preparation of fermented product: after mixing the raw materials prepared in step S1, add 15-20mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 15-20mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and ferment anaerobicly for 22h at 37°C. After the fermentation is completed, use 3% H2O2 solution to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product to obtain the desired fermented product; the fermented product is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicine with Lactobacillus acidophilus and Lactobacillus crispatus;

[0008] S4, preparation of Chinese medicine composition: take the fermentation product prepared in step S3, add 8 times amount of water, extract with hot water in a water bath at 95°C for 1.5h, extract twice, filter, combine the filtrate and concentrate to a crude drug concentration of 0.5g / mL, so as to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0009] Preferably: the components of the MRS agar medium used in step S2 of the present invention are: 10g peptone, 10g beef extract powder, 5g yeast extract powder, 20g glucose, 1g Tween, 5g sodium acetate, 2g triammonium citrate, 2g dipotassium hydrogen phosphate, 0.2g magnesium sulfate heptahydrate, 0.05g sulfuric acid tetrahydrate, 15g agar, 1000mL distilled water, and the pH is adjusted to 6.2-6.5; the components of the glucose blood liver agar medium are: 2.5g peptone, 1.5g beef extract, 10g glucose blood liver, 18g agar, 1000mL distilled water, and the pH value is 7.1±0.1.

[0010] Preferably, in step S1 of the present invention, the raw materials are crushed into 80-100 mesh particles using a crusher.

[0011] Preferably: the volume of the MRS liquid culture medium in step S2 of the present invention is 700 mL.

[0012] Preferably, in step S3 of the present invention, the volume of the bacterial solution of Lactobacillus acidophilus CICC-6082 and the bacterial solution of Lactobacillus crispatus CICC-25191 are both 20 mL.

[0013] Preferably: during the boiling process in step S4 of the present invention, stirring is required at regular intervals to prevent the medicinal materials from sticking to the pot or getting stuck to the bottom.

[0014] In a second aspect, the present invention provides a traditional Chinese medicine composition, which is prepared by the above-mentioned preparation method, and the traditional Chinese medicine composition has the effects of preventing hair loss, removing dandruff, relieving itching and anti-allergy.

[0015] In a third aspect, the Chinese medicine composition of the present invention is used in an anti-hair loss, anti-dandruff and anti-itching shampoo or pharmaceutical product.

[0016] Preferably, the Chinese medicine composition is added as an effective ingredient in an anti-hair loss, anti-dandruff and anti-itching shampoo or pharmaceutical product, and the added amount is 0.5-3%.

[0017] The present invention has the following beneficial effects:

[0018] 1. In the present invention, under the conditions of combined fermentation of Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191, the synergistic effect of Polygonum multiflorum, ginseng and Poria cocos has the effect of preventing hair loss.

[0019] 2. The synergistic effect of the tuckahoe fermented product and the mint fermented product in the present invention has the effect of removing dandruff and relieving itching.

[0020] 3. The synergistic effect of the Polygonum multiflorum fermented product, the ginseng fermented product and the egg white fermented product in the present invention has an anti-allergic effect.

[0021] 4. The shampoo made from the Chinese medicine composition of the present invention uses all Chinese medicine ingredients. Compared with some shampoos containing chemical ingredients, Chinese medicine shampoo is more gentle and safer and is suitable for people of all skin types.

[0022] 5. The Chinese medicine composition of the present invention is rich in a variety of plant nutrients, which can penetrate into the scalp hair follicles, replenish the nutrients required by the scalp and hair, and improve hair quality.

[0023] 6. The shampoo prepared from the Chinese medicine composition of the present invention adopts a pure plant formula, does not add any harmful chemical ingredients, is environmentally friendly, and is also healthier. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0025] Figure 1 This is the hair growth of mice in each group after 28 days of administration.

[0026] Figure 2 The results are as follows: The inhibitory effects of the Chinese medicine compositions of Example 2 and Comparative Examples 8-9 on bacteria.

[0027] Figure 3 This is the inhibitory effect of the Chinese medicine composition of Example 2 and Comparative Examples 8-9 on fungi.

[0028] Figure 4 This is the anti-allergic effect of the Chinese medicine composition of Example 3 and Comparative Examples 10-12.

[0029] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0030] Polygonum multiflorum, ginseng, Poria cocos, and mint were purchased from Anhui Kangweifu Pharmaceutical Co., Ltd.; ginger juice was purchased from Qingzhou Yikan Food Factory.

[0031] Example 1

[0032] This example demonstrates that under the conditions of combined fermentation of Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191, the synergistic effect of Polygonum multiflorum, ginseng and Poria cocos has the effect of preventing hair loss.

[0033] This embodiment provides a Chinese medicine composition, and the specific steps are as follows:

[0034] S1, raw material pretreatment: weigh 40g of Polygonum multiflorum, 15g of ginseng, 25g of Poria cocos, 10g of mint, and 50g of egg white; wash Polygonum multiflorum, ginseng, Poria cocos, and mint, dry them, and crush them into 100 mesh particles respectively. The purpose of washing and drying is to remove impurities and moisture;

[0035] S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from the China Industrial Microbiological Culture Collection. Both were inoculated in MRS agar medium and cultured at 37°C for 20 hours to activate the strains. Subsequently, they were transferred to 700 mL MRS liquid medium shake flasks and fermented for another 8 hours. As a result, the concentrations of the two bacterial solutions reached 2.0×10 8 cfu / mL. Both bacterial cultures were collected and stored in bacterial culture containers;

[0036] S3, preparation of fermented product: after mixing the raw materials prepared in step S1, add 20 mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and ferment anaerobicly for 22 h at 37° C. After the fermentation is completed, use 3% H2O2 solution to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product to obtain the desired fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicine with Lactobacillus acidophilus and Lactobacillus crispatus;

[0037] S4, preparation of Chinese medicine composition: take the fermentation product prepared in step S3, add 8 times amount of water, extract with hot water in a water bath at 95°C for 1.5h, extract twice, filter the two extracts, combine the filtrates and concentrate to a crude drug concentration of 0.5g / mL, to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0038] Comparative Example 1: In step S3, Polygonum multiflorum was not added, and the remaining steps were the same as those in Example 1.

[0039] Comparative Example 2: Ginseng is not added in step S3, and the remaining steps are the same as those in Example 1.

[0040] Comparative Example 3: In step S3, no Poria cocos is added, and the remaining steps are the same as those in Example 1.

[0041] Comparative Example 4: Lactobacillus acidophilus CICC-6082 was not added in step S2, and the remaining steps were the same as those in Example 1.

[0042] Comparative Example 5: Lactobacillus crispatus CICC-25191 was not added in step S2, and the remaining steps were the same as those in Example 1.

[0043] Comparative Example 6: Lactobacillus acidophilus CICC-6082 in step S2 was replaced by CICC-6091, and the remaining steps were the same as those in Example 1.

[0044] Comparative Example 7: In step S2, Lactobacillus crispatus CICC-25191 was replaced by CICC-24879, and the remaining steps were the same as those in Example 1.

[0045] Effects of Chinese herbal medicine combination on hair loss model mice

[0046] One hundred SPF-grade C57BL / 6N male mice were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. Before the experiment officially began, the mice were kept in a constant temperature and oxygen supply environment at 25±2℃ for one week. The entire experimental process strictly followed the national experimental animal management regulations. After one week of feeding, a hair loss model mouse was established. The specific method was as follows: paraffin and rosin were mixed in a volume ratio of 1:1 and heated to melt. After cooling to a suitable temperature, they were evenly applied to the back of the mouse. After the colloid solidified, it was quickly removed to remove the hair on the back of the mouse. The hair removal area was 4cm 2 .

[0047] One hundred mice were randomly divided into 10 groups, 10 mice in each group, and the drugs were administered at 8 am every day for 28 consecutive days.

[0048] Model group: No medication was given after modeling, and purified water (10 mL / kg) was applied at 8 am every day.

[0049] Positive control group (minoxidil group): After modeling, external spray was applied at 8 am every day. The specific method was to apply 5% minoxidil spray (0.12 mL / kg) on ​​the back of the mice.

[0050] Example group (Example 1 group): After modeling, apply the Chinese medicine composition externally at 8 o'clock every morning: Specific method: apply the corresponding 20% ​​v / v (diluted with purified water) Chinese medicine composition (10 mL / kg) to the depilated area on the back of the mouse, and massage with the fingertips for 5 minutes.

[0051] Comparative example group (Comparative example 1-7 groups): After modeling, externally apply the Chinese medicine composition at 8 am every day: Specific method: apply the corresponding 20% ​​v / v Chinese medicine composition (10 mL / kg) to the depilated area on the back of the mouse, and massage with the fingertips for 5 minutes.

[0052] After 28 days, the hair growth on the backs of mice in each group was observed and photographed. The experimental results are shown in Figure 1 After taking pictures, the mice were killed, and the skin of the depilatory area of ​​the same surface area on the back of the mice in the model group, the positive control group, the embodiment 1 group and the comparative example 1-7 groups was taken, the hair on the epidermis of the skin was scraped off with a scalpel, and the hair was accurately weighed to analyze the hair quality of the mice. The results are shown in Table 1.

[0053] Group Number of mice Mouse hair weight (g) Model Group 10 0.1171±0.02 Positive control group 10 0.1459±0.02 Example 1 Group 10 0.1438±0.02 Comparative Example 1 10 0.1299±0.02 Comparative Example 2 10 0.1295±0.02 Comparative Example 3 10 0.1297±0.02 Comparative Example 4 10 0.1293±0.02 Comparative Example 5 10 0.1302±0.02 Comparative Example 6 10 0.1312±0.02 Comparative Example 7 10 0.1315±0.02

[0054] Table 1 is the analysis table of mouse hair quality after 28 days of administration. Figure 1The hair growth of mice after 28 days of administration. The results show that hair grew on the backs of mice in all groups, and the hair growth in a small area of ​​the model group was slower and the hair weight was lighter. Compared with the model group, the hair growth of the comparative group (comparative example 1-7 groups) was relatively fast, and the hair weight increased relatively; the positive control group was not much different from the embodiment group (embodiment 1 group), with the fastest hair growth and the largest hair weight. The hair growth in the hair loss area of ​​the mice was lush and uniform, which confirmed that under fermentation conditions, the synergistic effect of Polygonum multiflorum, ginseng and Poria cocos has the effect of preventing hair loss.

[0055] Example 2

[0056] This example demonstrates that the synergistic effect of Poria cocos fermentation product and peppermint fermentation product has the effect of removing dandruff and relieving itching.

[0057] This embodiment provides a Chinese medicine composition, and the specific steps are as follows:

[0058] S1, raw material pretreatment: weigh 50g of Polygonum multiflorum, 20g of ginseng, 30g of Poria cocos, 15g of mint, and 55g of egg white; wash Polygonum multiflorum, ginseng, Poria cocos, and mint, dry them, and crush them into 100 mesh particles respectively. The purpose of washing and drying is to remove impurities and moisture;

[0059] S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from the China Industrial Microbiological Culture Collection. Both were inoculated in MRS agar medium and cultured at 37°C for 22 hours to activate the strains. Subsequently, they were transferred to 700 mL MRS liquid medium shake flasks and fermented for another 8 hours. As a result, the concentrations of the two bacterial solutions reached 2.1×10 8 cfu / mL. Both bacterial cultures were collected and stored in bacterial culture containers;

[0060] S3, preparation of fermented product: after mixing the raw materials prepared in step S1, add 20 mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and ferment anaerobicly for 22 h at 37° C. After the fermentation is completed, use 3% H2O2 solution to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product to obtain the desired fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicine with Lactobacillus acidophilus and Lactobacillus crispatus;

[0061] S4, preparation of Chinese medicine composition: take the fermentation product prepared in step S3, add 8 times amount of water, extract with hot water in a water bath at 95°C for 1.5h, extract twice, filter the two extracts, combine the filtrates and concentrate to a crude drug concentration of 0.5g / mL, to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0062] Comparative Example 8: Poria cocos was not added in step S3, and the remaining steps were the same as those in Example 2.

[0063] Comparative Example 9: Mint is not added in step S3, and the remaining steps are the same as those in Example 2.

[0064] In vitro antibacterial test

[0065] The test was carried out according to Appendix C4 "Test method for antibacterial performance of dissolution antibacterial (inhibitory) products" in GB15797-2002, the national standard of the People's Republic of China, "Sanitary Standard for Disposable Hygiene Products". 1.0 mL of the product to be tested was added to 0.1 mL of bacterial suspension (concentration of about 10 6 cfu / mL) and mixed evenly. After timing for 20 min, 9 mL of PBS was added and mixed thoroughly. Dilute to 2 dilutions, 1 mL was taken from each dish and placed in two parallel plates. Then, the corresponding culture medium was added and mixed and cultured respectively. Staphylococcus epidermidis (ATCC-49134, purchased from China Industrial Microorganism Culture Collection Center) was placed in nutrient agar medium and cultured at 37°C for 48 hours; Malassezia furfur (KWIK-STIK 2PK, purchased from China Industrial Microorganism Culture Collection Center) was placed in tryptic soytone agar medium and cultured at 30°C for 4 days; Staphylococcus aureus (CICC-10145, purchased from China Industrial Microorganism Culture Collection Center) was placed in tryptic soytone agar medium or nutrient agar medium and cultured at 37°C for 48 hours; Pseudomonas aeruginosa (CICC-21954, purchased from China Industrial Microorganism Culture Collection Center) was placed in tryptic soytone agar medium and cultured at 37°C for 48 hours; Candida albicans (ATCC-10231, purchased from Haibo Biotechnology Co., Ltd.) was placed in potato dextrose agar medium and cultured at 28°C for 72 hours, and the colonies were counted. The above test was repeated 3 times and the average value was taken. At the same time, a blank control and a positive control group (Tong Ren Tang Mao Yan Ning Shampoo) were set up.

[0066] Through the antibacterial test, the inhibitory effect of the sample to be tested on Staphylococcus epidermidis, Malassezia furfur, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans is determined. An antibacterial rate greater than 50% indicates that the product has an antibacterial effect, that is, an antidandruff effect. The higher the antibacterial rate, the better the effect.

[0067] Antibacterial rate calculation formula: X = (A-B) / A × 100%

[0068] In the formula: X---Inhibition rate %

[0069] A---Average colony recovery of control samples;

[0070] B - Average colonies recovered from test samples.

[0071] Figure 2 The inhibitory effect of the Chinese medicine composition of Example 2 and Comparative Examples 8-9 on bacteria, Figure 3 The results show that the inhibitory effect of the Chinese medicine composition of Example 2 and Comparative Examples 8-9 on fungi is significantly higher than that of the Comparative Example group and also better than that of the positive control group, indicating that the inhibitory effect of Example 2 is the best. It can be seen from the figure that the inhibition rate of Example 2 on microorganisms such as Malassezia furfur and Staphylococcus epidermidis is more than 80%, and it has a significant inhibitory and anti-dandruff effect, which confirms that the synergistic effect of the Poria cocos fermentation product and the mint fermentation product of Example 2 has a better anti-dandruff and antipruritic effect.

[0072] Example 3

[0073] This example demonstrates that the synergistic effect of the fermented product of Polygonum multiflorum, the fermented product of Ginseng and the fermented product of egg white has an anti-allergic effect.

[0074] This embodiment provides a Chinese medicine composition, and the specific steps are as follows:

[0075] S1, raw material pretreatment: weigh 60g of Polygonum multiflorum, 25g of ginseng, 35g of Poria cocos, 20g of mint, and 60g of egg white; wash Polygonum multiflorum, ginseng, Poria cocos, and mint, dry them, and crush them into 100 mesh particles respectively. The purpose of washing and drying is to remove impurities and moisture;

[0076] S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from the China Industrial Microbiological Culture Collection. Both were inoculated in MRS agar medium and cultured at 37°C for 24 hours to activate the strains. Subsequently, they were transferred to 700 mL MRS liquid medium shake flasks and fermented for another 8 hours. As a result, the concentrations of the two bacterial solutions reached 2.2×10 8 cfu / mL. Both bacterial cultures were collected and stored in bacterial culture containers;

[0077] S3, preparation of fermented product: after mixing the raw materials prepared in step S1, add 20 mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and ferment anaerobicly for 22 h at 37° C. After the fermentation is completed, use 3% H2O2 solution to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product to obtain the desired fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicine with Lactobacillus acidophilus and Lactobacillus crispatus;

[0078] S4, preparation of Chinese medicine composition: take the fermentation product prepared in step S3, add 8 times amount of water, extract with hot water in a water bath at 95°C for 1.5h, extract twice, filter the two extracts, combine the filtrates and concentrate to a crude drug concentration of 0.5g / mL, to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0079] Comparative Example 10: In step S3, Polygonum multiflorum was not added, and the remaining steps were the same as those in Example 3.

[0080] Comparative Example 11: Ginseng is not added in step S3, and the remaining steps are the same as those in Example 3.

[0081] Comparative Example 12: In step S3, no egg white is added, and the remaining steps are the same as those in Example 3.

[0082] Determination of anti-allergic efficacy by hyaluronidase inhibition in vitro

[0083] Experimental reagent preparation:

[0084] Acetate buffer solution (pH = 5.6): Measure 1.155 mL of glacial acetic acid and dilute to 100 mL, take 4.8 mL as solution A; weigh 2.72 g of sodium acetate crystals and dissolve in water to 100 mL, take 45.2 mL as solution B; mix solution A and solution B and dilute to 100 mL with water. Accurately measure the pH value and adjust to pH = 5.6 with solution A or solution B.

[0085] Ehrlich reagent: Weigh 0.8 g of p-dimethylaminobenzaldehyde and dissolve it in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol.

[0086] Acetylacetone solution: Dissolve 3.5 mL of acetylacetone in 50 mL of 1.0 mol / L sodium carbonate solution.

[0087] Both the hyaluronidase solution and the sodium hyaluronate solution can be directly prepared into corresponding concentrations using pH 5.6 acetate buffer solution, wherein the hyaluronidase was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., and the sodium hyaluronate was purchased from Guangzhou Pengyuan Chemical Co., Ltd.

[0088] In vitro anti-allergic test: 0.1mL of 0.25mmol / L CaCl2 solution and 0.5mL of 1250unit / mL hyaluronidase solution were mixed evenly and incubated at 37°C for 20min; then 0.5mL of sample solution was added and incubated at constant temperature for 20min; then 0.5mL of 0.5mg / mL sodium hyaluronate solution was added and kept warm for 40min; then 0.1mL of 0.4mol / L NaOH solution and 0.5mL of acetylacetone solution were added and heated in a boiling water bath for 15min, and then immediately cooled with ice water for 5min; finally, 1.0mL of Ehrlich reagent was added and diluted with 3.0mL of anhydrous ethanol, and the color was allowed to develop for 30min. The absorbance value was measured at a wavelength of 530nm, and the inhibition rate of the sample on hyaluronidase was calculated. The experimental results are shown in Figure 4 It is knowable.

[0089] Calculation formula:

[0090] Where: A is the ABS value of the control solution (using acetate buffer solution instead of sample solution);

[0091] B is the ABS value of the control blank solution (using acetate buffer solution instead of sample solution and enzyme solution);

[0092] C is the ABS value of the sample solution;

[0093] D is the ABS value of the sample blank solution (using acetate buffer solution instead of enzyme solution).

[0094] Figure 4 It is the anti-allergic effect of the Chinese medicine composition of Example 3 and Comparative Example 10-12. As can be seen from the figure, the anti-allergic effect of the Chinese medicine composition of Comparative Example 10-12 is less than 1.5%, which cannot play an anti-allergic effect, while the anti-allergic effect of the Chinese medicine composition of Example 3 is greater than 85%, which has a good anti-allergic effect, confirming that the synergistic effect of Polygonum multiflorum fermentation product, ginseng fermentation product and egg white fermentation product has an anti-allergic effect.

[0095] Example 4

[0096] This embodiment provides a Chinese medicine composition, and the specific steps are as follows:

[0097] S1, raw material pretreatment: weigh 50g of Polygonum multiflorum, 20g of ginseng, 30g of Poria cocos, 15g of mint, and 55g of egg white; wash Polygonum multiflorum, ginseng, Poria cocos, and mint, crush them, dry them, and crush them into 100 mesh particles respectively. The purpose of washing and drying is to remove impurities and moisture;

[0098] S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were purchased from the China Industrial Microbiological Culture Collection. Both were inoculated in MRS agar medium and cultured at 37°C for 22 hours to activate the strains. Subsequently, they were transferred to 700 mL MRS liquid medium shake flasks and fermented for another 8 hours. As a result, the concentrations of the two bacterial solutions reached 2.1×10 8 cfu / mL. Both bacterial cultures were collected and stored in bacterial culture containers;

[0099] S3, preparation of fermented product: after mixing the raw materials prepared in step S1, add 20 mL of Lactobacillus acidophilus CICC-6082 bacterial solution and 20 mL of Lactobacillus crispatus CICC-25191 bacterial solution prepared in step S2, and ferment anaerobicly for 22 h at 37° C. After the fermentation is completed, use 3% H2O2 solution to inactivate Lactobacillus acidophilus and Lactobacillus crispatus in the fermented product to obtain the desired fermented product, which is rich in various active substances and nutrients produced by the co-fermentation of Chinese herbal medicine with Lactobacillus acidophilus and Lactobacillus crispatus;

[0100] S4, preparation of Chinese medicine composition: take the fermentation product prepared in step S3, add 8 times amount of water, extract with hot water in a water bath at 95° C. for 1.5 h, extract twice, filter the extracts twice, combine the filtrates and concentrate to a crude drug concentration of 0.5 g / mL to obtain a Chinese medicine fermentation extract, add ginger juice at 5% v / v of the amount of the Chinese medicine fermentation extract, stir evenly to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

[0101] Application Example 1

[0102] The Chinese medicine composition obtained in Example 4 is added as an effective ingredient to an anti-hair loss, anti-dandruff and anti-itching shampoo product or medicament, and the addition amount of the Chinese medicine composition is 1%.

[0103] The above-mentioned embodiments only express the specific implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present invention. It should be pointed out that, for those skilled in the art, without departing from the concept of the present invention, any other changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principle of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A Chinese medicine composition, characterized in that: The Chinese medicine composition is obtained by fermenting a Chinese medicine mixture; the Chinese medicine mixture comprises 40-60 parts of Polygonum multiflorum, 15-25 parts of ginseng, 25-35 parts of Poria cocos, 10-20 parts of mint, and 50-60 parts of egg white by weight; the composite strain comprises Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191.

2. The Chinese medicine composition according to claim 1, characterized in that: Also contains ginger.

3. The Chinese medicine composition according to claim 1, characterized in that: The traditional Chinese medicine composition has the effects of preventing hair loss, removing dandruff, relieving itching and resisting allergy.

4. The method for preparing the Chinese medicine composition according to claim 1, characterized in that: The specific steps are as follows: S1. Raw material pretreatment: weigh the following raw materials: 40-60g of Polygonum multiflorum, 15-25g of ginseng, 25-35g of Poria cocos, 10-20g of mint, and 50-60g of egg white; wash the Polygonum multiflorum, ginseng, Poria cocos, and mint, dry them, and crush them; S2. Preparation of bacterial solution: Lactobacillus acidophilus CICC-6082 and Lactobacillus crispatus CICC-25191 were selected and inoculated into MRS agar medium respectively, and cultured at 37°C for 20-24h to obtain activated strains. The two activated strains were transferred to a shake flask containing 500-1000mL MRS liquid medium respectively, and the fermentation was continued for 8h, and the concentrations of the two bacterial solutions reached 2.0-2.2×10 8 cfu / mL, collect and store them in special bacterial liquid containers; S3, fermentation: the raw materials prepared in step S1 are mixed evenly, and then 15-20 mL of the bacterial solution of Lactobacillus acidophilus CICC-6082 and 15-20 mL of the bacterial solution of Lactobacillus crispatus CICC-25191 prepared in step S2 are added, and anaerobic fermentation is carried out at 37° C. for 22 hours. After the fermentation is completed, the Lactobacillus acidophilus and Lactobacillus crispatus in the fermentation product are inactivated using a 3% H2O2 solution to obtain the desired fermentation product. S4. Preparation of Chinese medicine composition: Take the fermented product prepared in step S3, add 8 times of water, and perform hot water extraction in a 95°C water bath for 1.5 hours, extract twice, filter, combine the filtrate and concentrate to a crude drug concentration of 0.5 g / mL to obtain a Chinese medicine composition; wherein the crude drug in the crude drug concentration refers to the sum of the masses of Polygonum multiflorum, ginseng, Poria cocos, mint and egg white.

5. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The components of the MRS agar medium used in step S2 are: 10 g of peptone, 10 g of beef extract powder, 5 g of yeast extract powder, 20 g of glucose, 1 g of Tween, 5 g of sodium acetate, 2 g of triammonium citrate, 2 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate heptahydrate, 0.05 g of manganese sulfate monohydrate, 15 g of agar, and 1000 mL of distilled water.

6. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: In the step S1, the raw materials need to be crushed into 80-100 mesh particles using a crusher.

7. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The volume of the Lactobacillus acidophilus CICC-6082 bacterial liquid in step S3 is 20 mL.

8. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The volume of the Lactobacillus crispatus CICC-25191 bacterial solution in step S3 is 20 mL.

9. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method of the Chinese medicine composition according to any one of claims 4 to 8 in an anti-hair loss, anti-dandruff and anti-itching shampoo or medicament, characterized in that: Application of the Chinese medicine composition in preparing Chinese medicine shampoo products.

10. The use according to claim 9, characterized in that: The Chinese medicine composition is added into the product as an effective ingredient, and the added amount is 0.5-3%.

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