Serum tube agglutination test auxiliary instrument and detection method used in immunological detection

Abstract

The present invention relates to a serum test tube agglutination test auxiliary instrument used in immunological detection and a detection method thereof, comprising: a base, on which a plurality of electronic droppers are arranged; a bearing plate, on which a plurality of test tubes are arranged; a deflection oscillation structure, connecting the test tubes and the bearing plate, one end of the deflection oscillation structure being formed with an abutment wheel; a telescopic rod, connecting the electronic dropper and the base, on which a supporting plate is arranged, the supporting plate cooperates with the abutment wheel to drive the test tube to move in a vertical direction of space; a driving plate, connected to the base, on which a switching groove is formed, after the test tube rises to a predetermined height, the switching groove cooperates with an engaging wheel connected to the supporting plate, so that the supporting plate and the abutment wheel can be misaligned; a switching mechanism, connecting the telescopic rod and the bearing plate, when the bearing plate moves to the end of the stroke, the switching structure can drive the telescopic rod to rotate a predetermined angle, thereby reducing the difficulty of operation during the test.

Description

Technical Field

[0001] The invention relates to a serum test tube agglutination test auxiliary instrument used in immunological detection and a detection method thereof. Background Art

[0002] Immunological detection methods play an important role in clinical diagnosis and research, helping doctors and researchers detect immune-related diseases, understand immune responses, and develop drugs and vaccines. Immunological detection methods generally include immunofluorescence, enzyme-linked immunosorbent assay, immunoelectrophoresis, immunoflow cytometry, serum agglutination test, etc.

[0003] Among them, serum agglutination tests are mostly performed by professional testing personnel. The test involves multiple steps, including but not limited to the addition of physiological saline, secondary dilution of the tested serum (continuous multiple dilution) and the addition of diagnostic bacterial solution. At the same time, in order to avoid the influence of accidental factors on the test results, multi-test tube tests are generally adopted, that is, each group of test tubes needs to manually perform the above steps. Obviously, the workload is huge, which affects the progress of the test and the accuracy of the results. Summary of the invention

[0004] The object of the present invention is to provide a serum tube agglutination test auxiliary instrument and a detection method thereof for use in immunological detection, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] Serum tube agglutination test auxiliary instruments used in immunological testing, including:

[0007] A base, on which a plurality of groups of electronic droppers are arranged;

[0008] A carrying plate connected to the linear drive module arranged on the base, and a plurality of test tubes are arranged on the carrying plate;

[0009] A deflection oscillation structure is connected to the test tube and the carrier plate, and an abutment wheel is formed at one end of the deflection oscillation structure. When the abutment wheel moves, the deflection oscillation structure can drive the test tube to perform an oscillation action;

[0010] A telescopic rod, connecting the electronic dropper and the base, wherein a supporting plate is arranged on the telescopic rod, and the supporting plate cooperates with the abutment wheel to drive the test tube to move in a vertical direction in space;

[0011] A driving plate connected to the base, a switching groove formed on the driving plate, and after the test tube rises to a predetermined height, the switching groove cooperates with the engaging wheel connected to the supporting plate, so that the supporting plate and the abutting wheel can be misaligned;

[0012] The switching mechanism connects the telescopic rod and the bearing plate. When the bearing plate moves to the end of the stroke, the switching structure can drive the telescopic rod to rotate a predetermined angle.

[0013] As a further solution of the present invention: the deflection oscillation structure comprises a plurality of slide grooves equidistantly arranged on the carrying plate, a slider is slidably installed in the slide groove, a connecting ring connected to the test tube is rotatably installed on the slider, a rotating shaft of the connecting ring passes through the slider and is connected to a rocker arm, and a guide wheel is rotatably installed at the end of the rocker arm;

[0014] The abutment wheel is rotatably mounted on the connecting ring and is coaxial with the rotating shaft of the connecting ring;

[0015] The deflection oscillation structure further includes a guide member arranged on the bearing plate, and the guide member is adapted to the guide wheel.

[0016] As a further solution of the present invention: the guide member is provided with a vertical portion and an inclined portion, and when the test tube rises and the guide wheel abuts against the inclined portion, the test tube can be kept in an inclined state;

[0017] A convex portion is formed at the connection between the vertical portion and the inclined portion.

[0018] As a further solution of the present invention: the telescopic rod includes a rotating sleeve rotatably mounted on the base and a lifting shaft slidably mounted in the rotating sleeve, the top of the lifting shaft is connected to the electronic dropper, and an annular groove is formed on the lifting shaft, a ring is rotatably sleeved on the annular groove, one side of the ring is connected to the electric telescopic rod arranged on the base, and the other side is connected to a connecting frame.

[0019] As a further solution of the present invention: a sliding connection portion is formed at the end of the connecting frame, and the sliding connection portion is slidably connected to the retardation groove arranged on the supporting plate;

[0020] The switching slot includes a first vertical slot and a second vertical slot which are arranged on the driving plate and are parallel to each other, wherein one end of the first vertical slot is connected to the second vertical slot through a horizontal slot, and the other end is connected through an inclined slot, and an extension slot is arranged on the extension line of the inclined slot toward the first vertical slot;

[0021] A deflection member is rotatably mounted on the upper end of the first vertical slot, and the deflection member is connected to the driving plate via a reset member.

[0022] As a further solution of the present invention: the switching mechanism includes:

[0023] An inner concave portion is arranged on the rotating sleeve, wherein the upper end surface of the inner concave portion is formed by staggered spiral surfaces, and an upper convex portion or a lower concave portion is formed at the connection between two adjacent spiral surfaces;

[0024] An energy storage component adapted to the spiral surface;

[0025] A trigger assembly is connected to the bearing plate and the rotating sleeve, and the trigger assembly can drive the rotating sleeve to rotate when the bearing plate moves to the end of the stroke.

[0026] As a further solution of the present invention: the energy storage assembly includes a vertical plate connected to the base, a sliding member is slidably mounted on the vertical plate, the sliding member and the vertical plate are connected by a spring, and a limiting wheel is arranged on the sliding member.

[0027] As a further solution of the present invention: the trigger assembly includes a ratchet wheel coaxially connected to the rotating sleeve, and the ratchet wheel is adapted to a ratchet plate connected to the bearing plate via a connecting frame.

[0028] A detection method of the serum tube agglutination test auxiliary instrument used in immunological detection as described above comprises the following steps:

[0029] Step 1: Load the cleaned test tube onto the deflection oscillation structure, and add a predetermined amount of diluted serum into the test tube at the end;

[0030] Step 2: Control the linear drive module to move in steps, so that each test tube moves to the bottom of the electronic dropper in turn. At this time, the telescopic rod drives the electronic dropper to move upward, and the test tube under the electronic dropper is tilted through the support plate, the abutment wheel and the deflection oscillation structure. Finally, the electronic dropper drips a fixed amount of physiological saline into each test tube. When the physiological saline is dripped into the next test tube, the support plate will continue to rise, and after the support plate is separated from the abutment wheel, the serum and physiological saline in the test tube are oscillated by the deflection oscillation structure.

[0031] Step 3: When the carrying plate moves to the end of the stroke, the telescopic rod drives the electronic dropper to switch positions;

[0032] Step 4: The linear drive module drives the test tube to move in the opposite direction. At this time, another set of electronic droppers will move downward following the telescopic rod and draw a quantitative mixture of serum and saline from the first test tube, and then drip it into another test tube. After the test tube is shaken, the electronic dropper will draw a quantitative mixture of serum and saline from the test tube and add it to another test tube, and so on. Then the linear drive module drives the test tube to move in the opposite direction again to reset, so that the telescopic rod rotates again, and the electronic dropper is switched again;

[0033] Step 5: The linear drive module drives the test tube to move again, and uses the switched electronic dropper to add diagnostic bacterial solution into each test tube, and makes each test tube vibrate.

[0034] Compared with the prior art, the present invention has the following beneficial effects:

[0035] By setting up the deflection oscillation structure and the telescopic rod, when the guide wheel moves upward, on the one hand, it can drive the test tube to tilt by cooperating with the inclined part, so that when the test liquid is dripped, the side wall of the test tube can be used to guide the test liquid, and the splashing of the test liquid is prevented, thereby improving the accuracy of each test liquid dosage. On the other hand, when the guide wheel follows the test tube to move downward under the action of gravity, it can cooperate with the raised part to drive the test tube to swing, thereby achieving the effect of oscillation mixing, and improving the mixing degree of the test liquid in the test tube. When the electronic dropper moves downward, the guide wheel will not move, and the center of gravity of the test tube is located below its rotating shaft, so that when the electronic dropper absorbs the test liquid, the position state of the test tube can be more stable. Based on the three movement states of the electronic dropper, basically all actions of manually dripping and transferring the test liquid are covered. It is not difficult to conclude that the use of this device can greatly simplify the workload of the operator during the test process.

[0036] The supporting plate can be lifted and lowered by the telescopic rod, the supporting plate and the driving plate, so as to drive the abutment wheel to rise, thereby driving the test tube to deflect. After the test liquid is dripped, the supporting plate can be dislocated from the abutment wheel, so that the test tube can oscillate autonomously. At the same time, when the supporting plate moves downward, it can also automatically reset, so that the supporting plate can repeat the above process, making the device reusable.

[0037] By setting up the switching mechanism, each group of electronic droppers is used to execute only one step, thereby preventing the electronic droppers from being contaminated and preventing the test liquid remaining on the electronic droppers from affecting the components in the test tubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 The present invention is a schematic structural diagram of an embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0039] Figure 2 The present invention is a schematic structural diagram from another angle of an embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0040] Figure 3 The present invention is a schematic structural diagram of a deflection oscillation structure in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0041] Figure 4 The present invention is a schematic structural diagram of another angle of the deflection oscillation structure in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0042] Figure 5 The structural explosion diagram of the deflection oscillation structure in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0043] Figure 6 The present invention is a schematic structural diagram of a switching mechanism and a telescopic rod in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0044] Figure 7 for Figure 6 A magnified view of the structure at center.

[0045] Figure 8 The present invention is a structural explosion diagram of a telescopic rod in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0046] Fig. 9 The present invention is a schematic structural diagram of a connecting frame, a supporting plate and a driving plate in one embodiment of a serum tube agglutination test auxiliary instrument used in immunological detection.

[0047] In the figure: 1, base; 2, linear drive module; 3, bearing plate; 301, slide groove; 4, slider; 5, connecting ring; 6, locking bolt; 7, abutment wheel; 8, rocker arm; 9, guide wheel; 10, guide member; 1001, inclined portion; 1002, vertical portion; 1003, raised portion; 11, connecting frame; 12, ratchet plate; 13, test tube; 14, rotating sleeve; 1401, spiral surface; 1402, upper convex portion; 1403, lower concave portion; 1404, limit block; 15, lifting shaft; 1501, limit slot; 1502, annular groove; 16, collar; 17, connecting frame; 1701, sliding connection; 18, supporting plate; 1801, hysteresis slot; 19, interlocking wheel; 20, driving plate; 2001, vertical slot No. 1; 2002, horizontal slot; 2003, vertical slot No. 2; 2004, inclined slot; 2005, extension slot; 21, deflection member; 22, electronic dropper; 23, vertical plate; 24, sliding member; 25, spring; 26, limit wheel; 27, electric telescopic rod; 28, side plate; 29, ratchet. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] In addition, when an element in the present invention is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only embodiment.

[0050] See also Figure 1 to Figure 9 In the embodiment of the present invention, the serum test tube agglutination test auxiliary instrument used in immunological detection includes: a base 1, a bearing plate 3, a deflection oscillation structure, a telescopic rod, a driving plate 20 and a switching mechanism, so that when the guide wheel 9 moves upward, on the one hand, it can drive the test tube 13 to tilt by cooperating with the inclined portion 1001, so that when the test liquid is dripped, the side wall of the test tube 13 can be used to guide the test liquid, and the test liquid splashing phenomenon is prevented, thereby improving the accuracy of each test liquid dosage. On the other hand, the guide wheel 9 follows the test tube 13 under the action of gravity. When the electronic dropper 22 moves downward, it can cooperate with the raised portion 1003 to drive the test tube 13 to swing, thereby achieving the effect of oscillation mixing, improving the mixing degree of the test liquid in the test tube 13, and when the electronic dropper 22 moves downward, the guide wheel 9 will not move, and the center of gravity of the test tube 13 is located below its rotation axis, so that when the electronic dropper 22 absorbs the test liquid, the position state of the test tube 13 is more stable, and based on the three movement states of the electronic dropper 22, it basically covers all the actions of manually dripping and transferring the test liquid. It is not difficult to conclude that the use of this device can greatly simplify the workload of the operator during the test process. The details are as follows:

[0051] The base 1 is provided with a plurality of electronic droppers 22;

[0052] The carrier plate 3 is connected to the linear drive module 2 disposed on the base 1, and a plurality of test tubes 13 are disposed on the carrier plate 3, wherein the linear drive module 2 can drive the test tubes 13 to perform stepping motion;

[0053] The deflection oscillation structure is connected to the test tube 13 and the carrier plate 3. An abutment wheel 7 is formed at one end of the deflection oscillation structure. When the abutment wheel 7 moves, the deflection oscillation structure can drive the test tube 13 to perform an oscillation action.

[0054] The deflection oscillation structure comprises a plurality of slide grooves 301 equidistantly arranged on the carrier plate 3, a slider 4 is slidably installed in the slide groove 301, a connecting ring 5 connected to the test tube 13 is rotatably installed on the slider 4, a rotating shaft of the connecting ring 5 passes through the slider 4 and is connected to a rocker arm 8, a guide wheel 9 is rotatably installed at the end of the rocker arm 8, a locking bolt 6 is arranged on the connecting ring 5, and the test tube 13 can be fixed by rotating the locking bolt 6;

[0055] The abutment wheel 7 is rotatably mounted on the connecting ring 5 and is coaxial with the rotating axis of the connecting ring 5;

[0056] The deflection oscillation structure further includes a guide member 10 disposed on the carrier plate 3, and the guide member 10 is adapted to the guide wheel 9;

[0057] The guide member 10 is provided with a vertical portion 1002 and an inclined portion 1001, and when the test tube 13 rises and the guide wheel 9 abuts against the inclined portion 1001, the test tube 13 can be kept in an inclined state;

[0058] A convex portion 1003 is formed at the connection between the vertical portion 1002 and the inclined portion 1001;

[0059] The telescopic rod is connected to the electronic dropper 22 and the base 1 . A supporting plate 18 is arranged on the telescopic rod. The supporting plate 18 cooperates with the abutting wheel 7 to drive the test tube 13 to move in the vertical direction of the space.

[0060] In the initial state, a predetermined amount of diluted serum is first added to the last test tube 13, and then the linear drive module 2 is controlled to move. The linear drive module 2 can drive the test tube 13 to do stepping motion through the carrier plate 3, and when the first test tube 13 moves to the top of the electronic dropper 22, the telescopic rod will drive the electronic dropper 22 to move upward, and at the same time drive the abutment wheel 7 to move upward through the supporting plate 18. At this time, the abutment wheel 7 drives the slider 4, the rocker arm 8 and the guide wheel 9 to move upward through the connecting ring 5. At this time, the guide wheel 9 will move along the vertical portion 1002, the raised portion 1003 and the inclined portion in sequence. The guide wheel 9 moves to the inclined portion 1001, and when the guide wheel 9 moves to the inclined portion 1001, the guide wheel 9 will drive the rocker arm 8 to deflect and make the test tube 13 deflect. At this time, the electronic dropper 22 drips physiological saline into the inclined test tube 13. Since the test tube 13 is in an inclined state, when the physiological saline is dripped, the side wall of the test tube 13 can guide the physiological saline, thereby preventing the physiological saline from splashing and flying to the outside of the test tube 13 during the process of dripping the physiological saline, causing the amount of physiological saline in the test tube 13 to be lower than the predetermined amount, thereby improving the accuracy of the physiological saline and the accuracy of the test results.

[0061] Furthermore, since a predetermined amount of serum is pre-dropped into the last test tube 13, it needs to be shaken and mixed after the physiological saline is added. At this time, the support plate 18 will continue to drive the abutment wheel 7 to rise, and the support plate 18 will gradually be misaligned with the abutment wheel 7. When the two are completely misaligned, the test tube 13 can move downward under the action of gravity. At this time, the guide wheel 9 will also reverse along the inclined portion 1001, the raised portion 1003 and the vertical portion 1002, so that the rocker arm 8 can drive the test tube 13 to oscillate back and forth several times to achieve mixing of the serum and the physiological saline.

[0062] In the operation of further diluting the serum, the telescopic rod will drive the electronic dropper 22 to move downward and sink below the liquid surface in the test tube 13 containing the mixed serum and saline, then absorb a certain amount of the serum and saline mixture and inject it into another test tube 13 already filled with saline to complete the further dilution of the serum, so that serum with a stepped concentration is obtained in each test tube 13. In this process, the electronic dropper 22 moves downward and the test tube 13 can remain stationary, which improves the stability of the test tube 13 during absorption.

[0063] It is worth noting that, in this embodiment, the telescopic rod drives the electronic dropper 22 to have three motion states, namely:

[0064] First, the test tube 13 is moved upward from the initial position to an inclined state. At this time, since the test tube 13 is in an inclined state, splashing is prevented when the test liquid is dripped, thereby improving the accuracy of the dripping amount of each test liquid;

[0065] Secondly, it moves upward from the initial position until the supporting plate 18 is separated from the abutting wheel 7. At this time, not only can the above-mentioned action of dripping the test liquid be completed, but also after the supporting plate 18 is separated from the abutting wheel 7, the cooperation between the guide wheel 9 and the protrusion 1003 can be used to realize the vibration of the test tube 13, thereby improving the mixing degree of the liquid components in the test tube 13. This movement state will only be performed when there are two or more test liquids in the test tube 13 to achieve a mixing effect;

[0066] Third, to move downward from the initial position, the electronic dropper 22 can be inserted into the test tube 13, and absorb the test solution in the test tube 13 to add it to another test tube 13, so as to realize the step-by-step dilution of the test solution.

[0067] Through the above arrangement, when the guide wheel 9 moves upward, on the one hand, it can drive the test tube 13 to tilt by cooperating with the inclined portion 1001, so that when the test liquid is dripped, the side wall of the test tube 13 can be used to guide the test liquid to prevent the test liquid from splashing, thereby improving the accuracy of each test liquid dosage. On the other hand, when the guide wheel 9 follows the test tube 13 to move downward under the action of gravity, it can cooperate with the raised portion 1003 to drive the test tube 13 to swing, thereby achieving an oscillation mixing effect, thereby improving the mixing degree of the test liquid in the test tube 13, and when the electronic dropper 22 moves downward, the guide wheel 9 will not move, and the center of gravity of the test tube 13 is located below its rotating shaft, so that when the electronic dropper 22 absorbs the test liquid, the position state of the test tube 13 can be more stable.

[0068] The three motion states of the electronic dropper 22 can be extended to cover all the actions of manually dripping and transferring the test liquid. It is not difficult to conclude that the use of the device can greatly simplify the workload of the operator during the test.

[0069] Taking into account that after the support plate 18 is separated from the abutment wheel 7, the test tube 13 falls too fast, causing the test tube 13 to separate from the connecting ring 5 and thus break into pieces, a damping layer may be added to the side wall of the slide 301 to suppress the descending speed of the slider 4 and the test tube 13. In order to ensure that the guide wheel 9 can move in accordance with the inclined portion 1001 when falling, the guide wheel 9 is preferably supported by a lead material.

[0070] See also Figure 6 , Figure 8 , Fig. 9 The telescopic rod comprises a rotating sleeve 14 rotatably mounted on the base 1 and a lifting shaft 15 slidably mounted in the rotating sleeve 14, the top of the lifting shaft 15 is connected to the electronic dropper 22, and an annular groove 1502 is formed on the lifting shaft 15, a collar 16 is rotatably sleeved on the annular groove 1502, one side of the collar 16 is connected to the electric telescopic rod 27 arranged on the base 1, and the other side is connected to the connecting frame 17, a sliding connection part 1701 is formed at the end of the connecting frame 17, and the sliding connection part 1701 is slidably connected to the retardation groove 1801 arranged on the supporting plate 18, specifically, the electric telescopic rod 27 is connected to the side plate 28 arranged on the base 1;

[0071] The rotating sleeve 14 and the lifting shaft 15 are connected via a limiting structure, wherein the limiting structure comprises a limiting block 1404 provided on the rotating sleeve 14 and a limiting groove 1501 provided on the lifting shaft 15, and the limiting block 1404 is slidably matched with the limiting groove 1501;

[0072] The driving plate 20 is connected to the side plate 28, and a switching groove is formed on the driving plate 20. After the test tube 13 rises to a predetermined height, the switching groove cooperates with the engaging wheel 19 connected to the supporting plate 18, so that the supporting plate 18 and the abutting wheel 7 can be misaligned. It should be noted that the supporting plate 18 and the driving plate 20 are both made of magnetic materials, and the two have the same magnetic poles and have mutual repulsion.

[0073] The switching slot includes a first vertical slot 2001 and a second vertical slot 2003 which are arranged on the driving plate 20 and are parallel to each other. One end of the first vertical slot 2001 is connected to the second vertical slot 2003 through a horizontal slot 2002, and the other end is connected through an inclined slot 2004. An extension slot 2005 is arranged on the extension line of the inclined slot 2004 toward the first vertical slot 2001.

[0074] A deflection member 21 is rotatably mounted on the upper end of the No. 1 vertical slot 2001, and the deflection member 21 is connected to the drive plate 20 via a reset member. Exemplarily, the reset member can be a coil spring installed on the rotating shaft of the deflection member 21 or two irregularly shaped magnetic poles arranged between the deflection member 21 and the drive plate 20.

[0075] In the initial state, due to the repulsive force between the supporting plate 18 and the driving plate 20, the supporting plate 18 is in a state of being away from the driving plate 20. At this time, the engaging wheel 19 is in the horizontal groove 2002 away from the end of the first vertical groove 2001. In this state, the projection of the supporting plate 18 and the abutment wheel 7 on the horizontal plane coincides, so that when the electric telescopic rod 27 drives the lifting shaft 15 to move upward, the supporting plate 18 can drive the abutment wheel 7 to move upward, and at this time, the engaging wheel 19 will move in the second vertical groove 2003. When the engaging wheel 19 moves to the upper end of the second vertical groove 2003, the test tube 13 can be lifted and is in an inclined state, and enters a state where the test liquid can be dripped. After the dripping of the test liquid is completed, the electric telescopic rod 27 will continue to drive the lifting shaft 15 to move upward. At this time, the engaging wheel 19 will move along the inclined groove 2004, pulling the supporting plate 18 relative to the connecting frame 1 7 slides, at this time, the support plate 18 will be partially misaligned with the abutment wheel 7, and when the embedded wheel 19 moves to the end of the inclined groove 2004, the support plate 18 will be completely misaligned with the connecting frame 17, and the test tube 13 will fall freely, while the connecting frame 17 will continue to rise and make the embedded wheel 19 move along the extension groove 2005. At the same time, when the embedded wheel 19 moves to abut against the deflection member 21, the embedded wheel 19 can drive the deflection member 21 to deflect until the embedded wheel 19 After moving to the extension groove 2005, the reset member can reset the deflection member 21, and then the engaging wheel 19 will move in the No. 1 vertical groove 2001 and maintain the complete misalignment state of the support plate 18 and the abutment wheel 7. When the engaging wheel 19 moves to the lower end of the No. 1 vertical groove 2001, the discharge force between the drive plate 20 and the support plate 18 can reset the support plate 18, so that the above process can be repeated when the support plate 18 moves upward next time.

[0076] Through the above arrangement, the supporting plate 18 can be raised and lowered to drive the abutment wheel 7 to rise, thereby driving the test tube 13 to deflect, and after the test liquid is dripped, the supporting plate 18 can be misaligned with the abutment wheel 7, so that the test tube 13 can oscillate autonomously. At the same time, the supporting plate 18 can automatically reset when moving downward, so that the supporting plate 18 can repeat the above process, making the device reusable.

[0077] See also Figure 3 , Figure 7 , Figure 8 , the switching mechanism connects the telescopic rod and the carrying plate 3, and when the carrying plate 3 moves to the end of the stroke, the switching structure can drive the telescopic rod to rotate a predetermined angle;

[0078] The switching mechanism comprises: an inner recess, an energy storage component and a trigger component.

[0079] The inner concave portion is arranged on the rotating sleeve 14, and the upper end surface of the inner concave portion is formed by staggered spiral surfaces 1401, and an upper convex portion 1402 or a lower concave portion 1403 is formed at the connection between two adjacent spiral surfaces 1401;

[0080] The energy storage assembly is adapted to the spiral surface 1401, and the energy storage assembly includes a vertical plate 23 connected to the base 1, a sliding member 24 is slidably mounted on the vertical plate 23, the sliding member 24 is connected to the vertical plate 23 by a spring 25, and a limiting wheel 26 is provided on the sliding member 24;

[0081] The trigger assembly connects the supporting plate 3 and the rotating sleeve 14. The trigger assembly can drive the rotating sleeve 14 to rotate when the supporting plate 3 moves to the end of the stroke. The trigger assembly includes a ratchet 29 coaxially connected to the rotating sleeve 14. The ratchet 29 is adapted to the ratchet plate 12 connected to the supporting plate 3 through the connecting frame 11.

[0082] In the present application, three groups of electronic droppers 22 are provided, which are used for the three steps of dripping physiological saline, transferring the mixture of serum and physiological saline, and dripping diagnostic bacterial solution during the test process. Each group of electronic droppers 22 performs specific steps, which can avoid the electronic dropper 22 from being contaminated, and prevent the test solution remaining on the electronic dropper 22 from affecting the components in the test tube 13.

[0083] Specifically, in the initial state, the limit wheel 26 is engaged with one of the recessed portions 1403, and the spring 25 is in a stretched state at this time. When the supporting plate 3 moves to the end of the stroke, the ratchet plate 12 can cooperate with the ratchet 29 to drive the rotating sleeve 14 to rotate, so that the limit wheel 26 can move along one of the spiral surfaces 1401 and the sliding member 24 moves upward, so that the spring 25 is further stretched. When the limit wheel 26 moves over the upper protrusion 1402, the ratchet plate 12 separates from the ratchet 29, and at the same time, the spring 25 releases its elastic potential energy and uses the other spiral surface 1401 to cooperate with the limit wheel 26 to actively drive the rotating sleeve 14 to rotate, so that the electronic dropper 22 located on the upper part of the test tube 13 is switched.

[0084] Specifically, the rotating sleeve 14 rotates 120° at a time.

[0085] Furthermore, when dropping physiological saline, the linear drive module 2 drives the carrier plate 3 to perform a unidirectional motion, and when transferring the mixture of serum and physiological saline and dropping the diagnostic bacterial solution, the carrier plate 3 performs a reciprocating motion, which is used to trigger the rotation of the rotating sleeve 14 on the one hand, and on the other hand, it can increase the number of oscillations of the test liquid and improve the mixing degree of the test liquid.

[0086] Through the above arrangement, each set of electronic droppers 22 is used to execute only one step, thereby preventing the electronic droppers 22 from being contaminated and preventing the test liquid remaining on the electronic droppers 22 from affecting the components in the test tube 13 .

[0087] As an embodiment of the present invention, a detection method of the serum tube agglutination test auxiliary instrument used in immunological detection is also proposed, comprising the following steps:

[0088] Step 1: Load the cleaned test tube 13 onto the deflection oscillation structure, and add a predetermined amount of diluted serum into the test tube 13 at the end;

[0089] Step 2: Control the linear drive module 2 to move, and the linear drive module 2 moves in steps, so that each test tube 13 moves to the bottom of the electronic dropper 22 in turn. At this time, the telescopic rod drives the electronic dropper 22 to move upward, and the test tube 13 located under the electronic dropper 22 is tilted through the support plate 18, the abutment wheel 7 and the deflection oscillation structure. Finally, the electronic dropper 22 drips a fixed amount of physiological saline into each test tube 13. When dripping physiological saline into the next test tube 13, the support plate 18 will continue to rise, and after the support plate 18 is separated from the abutment wheel 7, the deflection oscillation structure is used to oscillate the serum and physiological saline in the test tube 13;

[0090] Step 3: When the carrying plate 3 moves to the end of the stroke, the telescopic rod drives the electronic dropper 22 to switch positions;

[0091] Step 4: The linear drive module 2 drives the test tube 13 to move in the reverse direction. At this time, another set of electronic droppers 22 will move downward to follow the telescopic rod and absorb a quantitative mixture of serum and saline from the first test tube 13, and then drip it into another test tube 13. After the test tube 13 is shaken, the electronic dropper 22 will absorb a quantitative mixture of serum and saline from the test tube 13 and add it to another test tube 13, and so on. Then the linear drive module 2 drives the test tube 13 to move in the reverse direction again to reset, so that the telescopic rod rotates again, and the electronic dropper 22 switches again;

[0092] Step 5: The linear drive module 2 drives the test tubes 13 to move again, and uses the switched electronic dropper 22 to add diagnostic bacterial solution into each test tube 13, and makes each test tube 13 vibrate.

[0093] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0094] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A serum tube agglutination test auxiliary instrument used in immunological detection, characterized in that: include: A base (1), wherein a plurality of groups of electronic droppers (22) are arranged on the base (1); A carrying plate (3) connected to a linear drive module (2) arranged on the base (1), and a plurality of groups of test tubes (13) are arranged on the carrying plate (3); a deflection oscillation structure, connected to the test tube (13) and the carrier plate (3), wherein an abutment wheel (7) is formed at one end of the deflection oscillation structure, and when the abutment wheel (7) moves, the deflection oscillation structure can drive the test tube (13) to perform an oscillating action; A telescopic rod connected to the electronic dropper (22) and the base (1); a support plate (18) is provided on the telescopic rod; the support plate (18) cooperates with the abutment wheel (7) to drive the test tube (13) to move in a vertical direction in space; a driving plate (20) connected to the base (1), wherein a switching groove is formed on the driving plate (20); after the test tube (13) rises to a predetermined height, the switching groove cooperates with an engaging wheel (19) connected to the supporting plate (18), thereby enabling the supporting plate (18) and the abutting wheel (7) to be misaligned; a switching mechanism, connecting the telescopic rod and the bearing plate (3), wherein when the bearing plate (3) moves to the end of the travel, the switching mechanism can drive the telescopic rod to rotate by a predetermined angle; The deflection oscillation structure comprises a plurality of slide grooves (301) equidistantly arranged on the carrier plate (3), a slider (4) being slidably mounted in the slide groove (301), a connecting ring (5) connected to the test tube (13) being rotatably mounted on the slider (4), a rotating shaft of the connecting ring (5) passing through the slider (4) and being connected to a rocker arm (8), a guide wheel (9) being rotatably mounted on the end of the rocker arm (8); The abutment wheel (7) is rotatably mounted on the connecting ring (5) and is coaxial with the rotating shaft of the connecting ring (5); The deflection oscillation structure further comprises a guide member (10) arranged on the bearing plate (3), wherein the guide member (10) is adapted to the guide wheel (9).

2. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 1, characterized in that: The guide member (10) is provided with a vertical portion (1002) and an inclined portion (1001), and when the test tube (13) rises and the guide wheel (9) abuts against the inclined portion (1001), the test tube (13) can be kept in an inclined state; A protruding portion (1003) is formed at the connection between the vertical portion (1002) and the inclined portion (1001).

3. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 1, characterized in that: The telescopic rod comprises a rotating sleeve (14) rotatably mounted on the base (1) and a lifting shaft (15) slidably mounted in the rotating sleeve (14); the top of the lifting shaft (15) is connected to the electronic dropper (22); an annular groove (1502) is formed on the lifting shaft (15); a collar (16) is rotatably sleeved on the annular groove (1502); one side of the collar (16) is connected to an electric telescopic rod (27) arranged on the base (1); and the other side is connected to a connecting frame (17).

4. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 3, characterized in that: A sliding connection portion (1701) is formed at the end of the connecting frame (17), and the sliding connection portion (1701) is slidably connected to a retardation groove (1801) provided on the supporting plate (18); The switching slot comprises a first vertical slot (2001) and a second vertical slot (2003) which are arranged on the driving plate (20) and are parallel to each other, wherein one end of the first vertical slot (2001) is connected to the second vertical slot (2003) via a horizontal slot (2002), and the other end is connected via an inclined slot (2004), and an extension slot (2005) is arranged on the extension line of the inclined slot (2004) towards the first vertical slot (2001); A deflection member (21) is rotatably mounted on the upper end of the first vertical slot (2001), and the deflection member (21) is connected to the drive plate (20) via a reset member.

5. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 3, characterized in that: The switching mechanism comprises: An inner concave portion, arranged on the rotating shaft sleeve (14), wherein the upper end surface of the inner concave portion is formed by staggered spiral surfaces (1401), and an upper convex portion (1402) or a lower concave portion (1403) is formed at the connection between two adjacent spiral surfaces (1401); An energy storage component adapted to the spiral surface (1401); A trigger assembly is connected to the bearing plate (3) and the rotating sleeve (14), and the trigger assembly can drive the rotating sleeve (14) to rotate when the bearing plate (3) moves to the end of the stroke.

6. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 5, characterized in that: The energy storage assembly comprises a vertical plate (23) connected to the base (1), a sliding member (24) is slidably mounted on the vertical plate (23), the sliding member (24) and the vertical plate (23) are connected via a spring (25), and a limiting wheel (26) is provided on the sliding member (24).

7. The serum tube agglutination test auxiliary instrument used in immunological detection according to claim 5, characterized in that: The trigger assembly comprises a ratchet wheel (29) coaxially connected to the rotating sleeve (14), and the ratchet wheel (29) is adapted to a ratchet plate (12) connected to the bearing plate (3) via a connecting frame (11).

8. A detection method of the serum tube agglutination test auxiliary instrument used in immunological detection as claimed in claim 1, characterized in that: The following steps are involved: Step 1: Loading the cleaned test tube (13) onto the deflection oscillation structure, and adding a predetermined amount of diluted serum into the test tube (13) at the end; Step 2: Control the linear drive module (2) to move, and the linear drive module (2) moves in steps, so that each test tube (13) moves in sequence to the position directly below the electronic dropper (22). At this time, the telescopic rod drives the electronic dropper (22) to move upward, and the test tube (13) located below the electronic dropper (22) is tilted through the support plate (18), the abutment wheel (7) and the deflection oscillation structure. Finally, the electronic dropper (22) drips a fixed amount of physiological saline into each test tube (13). When the physiological saline is dripped into the next test tube (13), the support plate (18) will continue to rise, and after the support plate (18) is separated from the abutment wheel (7), the serum and physiological saline in the test tube (13) are oscillated by the deflection oscillation structure. Step 3: When the carrier plate (3) moves to the end of the stroke, the telescopic rod drives the electronic dropper (22) to switch positions; Step 4: the linear drive module (2) drives the test tube (13) to move in the reverse direction, at which time another set of electronic droppers (22) will move downwards following the telescopic rod and draw a quantitative mixture of serum and saline from the first test tube (13), and then drip it into the other test tube (13). After the test tube (13) is shaken, the electronic dropper (22) draws a quantitative mixture of serum and saline from the test tube (13) and adds it to the other test tube (13), and so on. Then, the linear drive module (2) drives the test tube (13) to move in the reverse direction again to reset, so that the telescopic rod rotates again, and the electronic dropper (22) is switched again. Step 5: The linear drive module (2) drives the test tubes (13) to move again, and uses the switched electronic dropper (22) to add diagnostic bacterial solution into each test tube (13), and causes each test tube (13) to vibrate.

Images