Temperature control device and sample processing equipment
By designing multiple temperature control areas and flow guide movement in the PCR device to achieve rapid temperature change, the problem of slow temperature switching rate in the prior art is solved, and the efficiency and accuracy of PCR reaction are improved.
Patent Information
- Application Number
- CN202411653657.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-19
- Publication Date
- 2025-01-07
AI Technical Summary
In the existing PCR temperature control scheme, the temperature switching rate is slow, which affects the efficiency of DNA amplification, leads to low detection efficiency, and may interfere with the DNA amplification process.
A temperature control device is designed to form multiple temperature control areas with different temperatures using a thermal conductivity base and heating components, and to achieve rapid temperature change through the movement of the flow guide in the circulation channel, improving the accuracy of temperature switching and reaction efficiency.
The rapid temperature change in the PCR reaction is achieved, the accuracy of temperature switching and the accuracy of experimental results are improved, and the efficiency of the PCR reaction is improved.
Smart Images
Figure CN119265030A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of medical devices, and in particular to a temperature control device and a sample processing device. Background Art
[0002] Polymerase Chain Reaction (PCR) is an important technology widely used in biomedical engineering, molecular biology, chemical engineering and counting. PCR technology controls the temperature to denature DNA into single strands at high temperatures. At low temperatures, primers and single strands combine according to the principle of complementary base pairing. The temperature is then adjusted to the optimal reaction temperature of DNA polymerase (about 72°C), thereby achieving rapid DNA amplification.
[0003] In the temperature control scheme of the prior art, PCR is achieved by switching different temperatures through a temperature control module controlled by a complex algorithm. However, the temperature switching rate of this scheme is slow, which affects the amplification efficiency of DNA, resulting in low PCR detection efficiency. In addition, during the temperature switching process, the PCR reaction time is too long, which may interfere with the DNA amplification process and affect the reaction results. Summary of the invention
[0004] In order to overcome the problem in the prior art that the PCR reaction is affected by the slow temperature switching rate, the purpose of the present invention is to provide a temperature control device and a sample processing device to speed up the PCR reaction time.
[0005] In a first aspect, the present invention provides a temperature control device, comprising: a heat-conducting base, wherein the heat-conducting base has a closed-loop circulation channel and a fluid inlet connected to the circulation channel, wherein the fluid inlet is used to introduce fluid; at least two flow guides, each of which is arranged in the circulation channel and can move along the closed-loop direction of the circulation channel, and an independent fluid receiving space is formed between two adjacent flow guides; at least two heating components arranged on the heat-conducting base, each of which forms an independent plurality of temperature control areas with different temperatures on the heat-conducting base, and the circulation channel passes through each of the temperature control areas in its closed-loop direction; and a driving component, which is used to drive each of the flow guides to circulate in the circulation channel so that each of the fluid receiving spaces can be placed in each of the temperature control areas.
[0006] Compared with the prior art, the temperature control device provided by the present invention has at least the following beneficial effects: In this temperature control device, the heat conduction effect of the heat conduction base is utilized, and the heating component forms a plurality of temperature control areas with different temperatures on the heat conduction base, so that the fluid in the circulation channel will have different temperature effects due to different temperature control areas, and can circulate in different temperature control areas under the flow guiding effect of the flow guide, and can quickly realize the temperature change process, especially for the liquid in the PCR reaction, the constant temperature effect in different areas can greatly improve the temperature accuracy during the reaction, reduce the influence of the temperature switching process on the test results, and improve the accuracy of the experimental results. At the same time, the multiple independent fluid storage spaces formed by the flow guide in the circulation channel can accommodate fluids that do not affect each other, and are in different temperature control areas when the fluid reacts, and the reaction process is carried out at the same time, thereby improving the reaction efficiency.
[0007] In a preferred embodiment of the present invention, the driving component can drive each of the flow guides so that each of the fluid receiving spaces is placed in only one corresponding temperature control area, and at this time, there is a corresponding flow guide between two adjacent temperature control areas.
[0008] In a preferred embodiment of the present invention, the circulation channel is in a circular ring shape, and two adjacent guide members form a first angle with the center of the circulation channel as the vertex, and the second angle formed by the fan ring part of the circulation channel in each temperature control area with the center of the circle as the vertex is an integer multiple of the first angle.
[0009] In a preferred embodiment of the present invention, the flow guide is a spherical component, and the diameter of the spherical component is the same as or close to the width of the circulation channel; The driving component comprises a driving plate and a power source for rotating the driving plate. The driving plate abuts against each of the flow guides and drives each of the flow guides to move on the circulation channel when rotating.
[0010] In a preferred embodiment of the present invention, the thermal conductive base has a closed fluid channel, the temperature control device also includes a sealing film, the sealing film covers the fluid channel to form the circulation channel, and the guide member squeezes the sealing film from the outside of the sealing film relative to the fluid channel and is placed in the fluid channel.
[0011] In a second aspect, the present invention further provides a sample processing device, comprising: A lysis module, the lysis module comprising a lysis chamber, the lysis chamber having a sample inlet for introducing the sample; An extraction module, the extraction module comprising an extraction chamber; A washing module, the washing module comprising a washing chamber; An amplification module, comprising the temperature control device described in the embodiment of the first aspect; A reading module, used for reading and analyzing the amplification result signal in the amplification module; The lysis chamber, the extraction chamber, the washing chamber and the circulation channel of the temperature control device are fluidically connected in sequence.
[0012] The sample processing device provided by the present invention has at least the following beneficial effects: The sample processing device integrates multiple modules for PCR reaction in the same device. The sample releases nucleic acid after reacting with the lysis solution in the lysis module. After entering the extraction module to extract DNA or RNA, the sample goes to the washing module to eliminate the lysis solution and the extraction solution. The sample then enters the amplification module to circulate in different temperature-controlled areas. After a rapid reaction, the reading module reads and analyzes the sample amplification. The multiple modules are independent of each other and utilize the fluid channels between them to sequentially transmit between the modules, thereby improving the PCR amplification efficiency, accelerating the PCR reaction time, and improving the PCR detection efficiency.
[0013] In a preferred embodiment of the present invention, it also includes an equipment body, which has a fluid channel. The lysis chamber, the extraction chamber, the washing chamber and the circulation channel are arranged on the equipment body along the fluid flow direction of the fluid channel, and the fluid channel is provided with a fluid valve between each chamber.
[0014] In a preferred embodiment of the present invention, the fluid valve is a valve composed of a chemical substance that can be opened by heating, or a black film that can be opened by laser.
[0015] In a preferred embodiment of the present invention, a sealing film is further included, and the sealing film covers the lysis chamber, the extraction chamber, the washing chamber, the circulation channel and the fluid channel to form a fluid circulation system in the device body.
[0016] In a preferred embodiment of the present invention, at least two of the heating components include a first heating component and a second heating component, the temperature of the temperature control area formed by the first heating component on the thermally conductive base is a first temperature, the temperature of the temperature control area formed by the second heating component on the thermally conductive base is a second temperature, and the second temperature is lower than the first temperature.
[0017] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by implementing the technical solution of the present invention. The purpose and other advantages of the present invention can be achieved and obtained by the structures and / or processes specifically pointed out in the description, claims and drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1A schematic diagram of the structure of a temperature control device provided by an embodiment of the first aspect of the present invention;
[0019] Figure 2 A schematic diagram of the structure of a temperature control device provided by one embodiment of the present invention;
[0020] Figure 3 A schematic diagram of the structure of a temperature control device provided by another embodiment of the present invention in a first state;
[0021] Figure 4 A schematic diagram of the structure of a temperature control device provided by another embodiment of the present invention in a second state;
[0022] Figure 5 A schematic diagram of the structure of a sample processing device provided by an embodiment of the second aspect of the present invention;
[0023] Figure 6 An exploded view of a sample processing device including a temperature control device provided by an embodiment of the present invention;
[0024] Figure 7 A schematic diagram of the structure of a device body provided in an embodiment of the present invention;
[0025] Figure 8 This is a schematic diagram of the structure of various modules of the sample processing device provided by an embodiment of the present invention.
[0026] Description of Figure Numbers: 10 heat-conducting base, 11 first temperature control area, 12 second temperature control area, 13 third temperature control area, 20 driving component, 21 power source, 22 driving plate, 30 flow guide, 31 first flow guide, 32 second flow guide, 33 third flow guide, 34 fourth flow guide, 40 heating component, 41 first heating component, 42 second heating component, 50 circulation channel, 60 fluid receiving space, 70 fluid inlet, 80 sealing film; 100 device body, 110 fluid channel; 200 lysis module, 210 lysis chamber, 220 sample inlet; 300 extraction module, 310 extraction chamber; 400 washing module, 410 washing module; 500 amplification modules. DETAILED DESCRIPTION
[0027] The following will describe the implementation methods of the present invention in detail in combination with the accompanying drawings and embodiments, so that the implementation process of how the present invention applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly. It should be noted that these specific descriptions are only for ordinary technicians in this field to understand the present invention more easily and clearly, rather than a restrictive interpretation of the present invention; for example, the first and second mentioned in the embodiments of the present invention do not constitute a limitation thereto, but are only for expressing the serial numbers of multiple identical or similar devices and mechanisms. Ordinary technicians in this field can also readjust these serial numbers for the convenience of expression or in the process of arranging technical solutions; and alternative solutions are described for some mechanisms in different embodiments, and these alternatives can also be applied to other identical or similar devices and mechanisms; and as long as there is no conflict, the various embodiments in the present invention and the various features in each embodiment can be combined with each other, and the technical solutions formed are all within the protection scope of the present invention.
[0028] The technical solution of the present invention is described in detail below through the accompanying drawings and specific embodiments.
[0029] See also Figure 1 A temperature control device includes a heat-conducting base 10, a driving component 20, at least two flow guides 30 and at least two heating components 40. The heat-conducting base 10 is made of a material with heat conductivity such as metal, heat-conducting polymer, etc., so that it has good heat conduction ability. The heat-conducting base 10 has a closed-loop circulation channel 50 and a fluid inlet 70 connected to the circulation channel 50. The fluid enters the circulation channel 50 through the fluid inlet 70 and can circulate in the circulation channel 50. Each flow guide 30 is arranged in the circulation channel 50, can move along the closed-loop direction of the circulation channel 50, guide the fluid to circulate in the circulation channel 50, and an independent fluid receiving space 60 is formed between adjacent flow guides 30, so that the flow guide 30 acts as a movable barrier to block between two adjacent fluid receiving spaces 60, and each fluid receiving space 60 independently receives a corresponding fluid. Each heating component 40 is disposed on the heat-conducting base 10, and directly exchanges heat with the heat-conducting base 10, forming a plurality of temperature-controlled areas with different temperatures on the heat-conducting base 10, wherein in this embodiment, one heating component 40 corresponds to one temperature-controlled area, and under the temperature control of different heating components 40, the temperatures of the temperature-controlled areas are inconsistent, and can all be maintained at their respective set temperatures. The driving component 20 is used to drive the flow guide 30 to move in the circulation channel 50, so that each fluid receiving space 60 moves to the corresponding temperature-controlled area with the movement of the flow guide 30, and the fluid in the fluid receiving space 60 reaches the reaction temperature in the temperature-controlled area.
[0030] In one embodiment, the heat-conducting base 10 can be formed by an integral molding method, and the circulation channel 50 is formed on the heat-conducting base 10 during the molding process. Figure 6 The circulation channel 50 is formed on a surface of the thermal conductive base 10 to form a fluid channel on the surface. The fluid channel is connected end to end on the thermal conductive base 10 to form a closed structure. The portion enclosed by the closed structure on the thermal conductive base 10 is the inner area, and the portion outside the closed structure on the thermal conductive base 10 is the outer area. The side wall of the fluid channel adjacent to the inner area is the inner side wall, and the side wall adjacent to the outer area is the outer side wall. The fluid inlet 70 is arranged on the outer side wall, so as to introduce the fluid in the outer area into the fluid channel.
[0031] In order to seal the fluid channel, the temperature control device may further be provided with a sealing film 80, which covers the fluid channel to form a circulation channel 50. The flow guide 30 squeezes the sealing film 80 from the outside of the fluid channel relative to the sealing film 80 and is placed in the fluid channel, which can prevent the fluid flowing into the fluid channel from flowing out from the outer opening of the fluid channel. The sealing film 80 may be a PE film, or other membrane structures that can seal the fluid, which are not limited here. In addition, the heat-conducting base 10 may also be formed with a circulation channel 50 in its interior in a sealed and closed manner using a cover structure, which is assembled from a substrate and a sealing cover. After the fluid channel is formed on the substrate, the sealing cover is placed on the substrate and seals the fluid channel, thereby forming a circulation channel 50 inside the heat-conducting base 10.
[0032] In another embodiment, the circulation channel 50 is in the shape of a ring, and two adjacent guide members 30 form a first angle with the center of the circulation channel 50 as the vertex, and the second angle formed by the fan ring part of the circulation channel 50 in each temperature control zone with the center of the circle as the vertex is an integer multiple of the first angle, so that when each guide member 30 moves synchronously on the circulation channel 50, if one of the guide members 30 moves to the adjacent position between two adjacent temperature control zones, the fluid receiving space 60 formed between each guide member 30 is only in one of the temperature control zones and will not cross into other temperature control zones.
[0033] by Figure 1 and Figure 2Taking the embodiment shown in as an example, the guide member includes a first guide member 31 and a second guide member 32, and the heat-conducting base 10 has two temperature-controlled areas, namely, the first temperature-controlled area 11 and the second temperature-controlled area 12. The first temperature-controlled area 11 is temperature-controlled by the first heating component 41, and the second temperature-controlled area 12 is temperature-controlled by the second heating component 42, wherein the first heating component 41 and the second heating component 42 are respectively heat-exchanged with the first temperature-controlled area 11 and the second temperature-controlled area 12 by directly contacting the heat-conducting base 10. The dividing line between the first temperature-controlled area 11 and the second temperature-controlled area 12 is a straight line and passes through the circle of the circulation channel 50, so that the second angles occupied by the first temperature-controlled area 11 and the second temperature-controlled area 12 on the circulation channel 50 are both 180°, and the dividing line forms a first dividing point and a second dividing point set oppositely on the circulation channel 50. The first angle formed between the first guide member 31 and the second guide member 32 with the center of the circulation channel 50 as the vertex is 180°, and the first guide member 31 and the second guide member 32 form a first fluid receiving space and a second fluid receiving space on opposite sides of the circulation channel 50, so that the first fluid receiving space and the second fluid receiving space on the circulation channel 50 are both presented as a 180° fan ring structure. Under this precondition, when the first guide member 31 is located at the first dividing point, the second guide member 32 is located at the second dividing point, at this time, the first fluid receiving space is located in the first temperature control area 11, and the second fluid receiving space is located in the second temperature control area 12; or, when the first guide member 31 is located at the second dividing point, the second guide member 32 is located at the first dividing point, at this time, the first fluid receiving space is located in the second temperature control area 12, and the second fluid receiving space is located in the first temperature control area 11. Except when the second angle and the first angle are both 180°, the two fluid receiving spaces 60 are located in two temperature-controlled spaces respectively. When the first angle and the second angle occupied by the adjacent flow guides are equal, it can be imagined that each time one of the flow guides is located at the boundary point of two adjacent temperature-controlled areas, the remaining flow guides are located in each boundary point in sequence, so that each fluid receiving space 60 is located in a different temperature-controlled area. When the temperature is switched, each flow guide is synchronously rotated through the first angle and is located at the next boundary point in the transmission direction, and each fluid receiving space 60 moves to the next temperature-controlled area accordingly.
[0034] It can be expanded that when the second angle is an integer multiple of the first angle, the above effect can be achieved, and the temperature control area can be designed to accommodate two or more fluid storage spaces. Figure 1 , Figure 3 and Figure 4The structure shown in is used as an embodiment, and three temperature control areas are arranged on the heat conductive base 10, the second angle occupied by the first temperature control area 11 is 180°, the second angle occupied by the second temperature control area 12 and the third temperature control area 13 is 90°, and there are four flow guides, and the first angles formed between adjacent ones are all 90°, and four fluid receiving spaces are formed. In the first reaction state of this embodiment, refer to Figure 3 The first fluid receiving space and the second fluid receiving space are located in the first temperature control area 11, the third fluid receiving space is located in the second temperature control area 12, and the fourth fluid receiving space is located in the third temperature control area 13. The first guide member 31 is located at the dividing point between the first temperature control area 11 and the second temperature control area 12, the third guide member 33 is located at the dividing point between the first temperature control area 11 and the third temperature control area 13, the fourth guide member 34 is located between the first guide member 31 and the third guide member 33 and is located in the first temperature control area 11, the second guide member 32 is located at the dividing point between the second temperature control area 12 and the third temperature control area 13, and the liquid in each fluid receiving space reacts in the corresponding temperature control area. When the above-mentioned guide members rotate synchronously by 90°, the first fluid receiving space and the fourth fluid receiving space are located in the first temperature control area 11, the second fluid receiving space is located in the second temperature control area 12, the third fluid receiving space is located in the third temperature control area 13, the fourth guide member 34 is located at the dividing point between the first temperature control area 11 and the third temperature control area 13, the third guide member 33 is located between the fourth guide member 34 and the second guide member 32 and is located in the first temperature control area 11, the first guide member 31 is located at the dividing point between the second temperature control area 12 and the third temperature control area 13, and the liquid in each fluid receiving space reacts in the corresponding temperature control area. Therefore, when the angle of the synchronous operation of each guide member is an integer multiple of the first angle, each fluid space can be located in the corresponding temperature control area to react without the phenomenon of crossing the temperature control area.
[0035] In some implementations of the above embodiments, the driving component 20 can drive each guide member 30 so that each fluid receiving space 60 is placed only in a corresponding temperature control area, and at this time, there is a corresponding guide member 30 between two adjacent temperature control areas. The driving component 20 can be an independent driving member, and can be composed of multiple driving members. The guide member 30 can be driven to the target position by a common driving member or an independent driving member. When each guide member 30 is driven by an independent driving member, each driving member can drive each guide member 30 to a corresponding position according to program selection or other control methods, so that the fluid receiving space 60 is located in the target temperature control area. Of course, a driving member can also be provided to drive the guide members 30 to move separately, and each guide member 30 can be driven one by one in a sequential moving manner to move it to the target position.
[0036] In one embodiment, in combination Figures 1 to 6 In the embodiment of the present invention, the driving component 20 can simultaneously drive each flow guide member 30 to move synchronously. Figure 6 , the fluid channel is sealed by the sealing film 80 to form a circulation channel 50, the cross section of the side wall of the fluid channel is arc-shaped, the flow guide 30 is set as a spherical component, and the diameter of the spherical component is the same as or close to the width of the circulation channel 50, so that when the flow guide 30 is set in the fluid channel, it can prevent the fluid interaction between the two adjacent fluid receiving spaces 60. The driving component 20 includes a driving plate 22 and a power source 21 for rotating the driving plate 22. The driving plate 22 approaches or is attached to the thermal conductive base 10, and at the same time abuts against each flow guide 30 through the sealing film 80. When the power source 21 drives the driving plate 22 to rotate, the driving plate 22 drives each flow guide 30 to move in the fluid channel synchronously through the abutment with the flow guide 30, and the moving angle is the same, thereby realizing the synchronous movement of the above-mentioned flow guides 30. Among them, the power source 21 is preferably a motor, and of course other power components can also be used, which will not be repeated here. This embodiment utilizes an external driving component 20 to drive the flow guide member 30 to move in the circulation channel 50 while ensuring the sealing of the circulation channel 50. The structure is simple and easy to implement, and the design is ingenious.
[0037] In a second aspect of the present invention, a sample processing device is provided. Figures 5 to 8 , including a lysis module 200, an extraction module 300, a washing module 400, an amplification module 500 and a reading module, the lysis module 200 includes a lysis chamber 210, the lysis chamber 210 has a sample inlet 220 for introducing samples, the extraction module 300 has an extraction chamber 310, the washing module 400 includes a washing chamber 410, the amplification module 500 includes the temperature control device in the first aspect embodiment, and the reading module is used to read and analyze the amplification result signal in the amplification module 500, wherein the lysis chamber 210, the extraction chamber 310, the washing chamber 410 and the circulation flow channel 50 in the amplification module 500 are fluidically connected in sequence, so that the inlet entering from the sample inlet 220 passes through the lysis chamber 210, the extraction chamber 310, the washing chamber 410 and the circulation flow channel 50 in sequence, and is read and analyzed by the reading module after the reaction is completed.
[0038] During the specific implementation of the sample processing device, the lysis module 200 is used to lyse the cell sample in the sample, and the lysis solution is stored in the lysis chamber 210. After the sample enters the lysis chamber 210 from the sample inlet 220, it reacts with the lysis solution to release nucleic acid to form a mixed solution composed of the sample and the lysis solution. The mixed solution sequentially flows downward into the extraction chamber 310 of the extraction module 300, and the extraction solution enters the mixed solution to extract DNA or RNA from the mixed solution. Subsequently, the mixed solution enters the washing chamber 410 of the washing module 400, and the washing solution eliminates the chemical components of the lysis solution and the extraction solution. After washing, the mixed solution flows into the amplification module 500, and is moved to each temperature control area under the action of the guide member 30 to perform corresponding reactions. After amplification, an amplification liquid is formed, and the amplified DNA or RNA in the amplification liquid has an amplification result signal that can be read and analyzed by the reading module.
[0039] Further reading Figures 5 to 8 The sample processing device also includes a device body 100, a lysis module 200, an extraction module 300, a washing module 400, an amplification module 500 and a reading module are all arranged on the device body 100, and a heat conductive base 10 is formed on the device body 100. The lysis chamber 210, the extraction chamber 310 and the washing chamber 410 can be formed in an integrated manner on the device body 100. The device body 100 has a fluid channel 110, and the lysis chamber 210, the extraction chamber 310, the washing chamber 410 and the circulation channel 50 are arranged on the device body 100 along the fluid flow direction of the fluid channel 110. After the sample enters from the sample inlet 220, the mixed liquid formed with the lysis solution and the like enters the circulation channel 50 through the fluid channel 110.
[0040] Specifically, the lysis solution, extraction solution and washing solution can be stored in the lysis chamber 210, extraction chamber 310 and washing chamber 410 in advance, respectively. Of course, the lysis solution can also be stored in a chamber different from the lysis chamber 210. When the sample arrives at the lysis chamber 210, the lysis module 200 injects the lysis solution into the lysis chamber 210, so that the mixed solution composed of the lysis solution and the sample reacts in the lysis chamber 210, and the extraction solution and the washing solution can also be subsequently injected into the corresponding reaction chamber using the aforementioned lysis solution setting method, thereby realizing the cyclic processing of different samples.
[0041] In addition, the sample processing device may further include a driving module, and the driving module is used to drive the mixed solution to move in the lysis chamber 210, the extraction chamber 310, the washing chamber 410 and the fluid channel 110. In this embodiment, the driving module includes a movable magnetic attraction component, the injected lysis solution contains magnetic beads, and the magnetic attraction component has a magnetic attraction member made of a magnetic attraction material such as a magnet. The magnetic attraction component moves outside the fluid channel 110 under the action of a driving source, and when moving, the mixed solution is transferred in the fluid channel 110 by attracting the magnetic beads to guide the flow of the mixed solution, which can effectively guide the flow process of the mixed solution and improve the fluid movement efficiency.
[0042] In some embodiments, the fluid channel 110 is provided with a fluid valve between each cavity, and the fluid valve is used to prevent the mixed liquid from flowing into the next chamber when the sample reacts in the corresponding chamber, which is conducive to the effective reaction in the chamber. Among them, the fluid valve can be set as a valve composed of chemical substances that is opened by heating, and is heated by a heating element set on the device to open the valve, and the valve can also be set to close when the temperature drops to room temperature, thereby realizing the opening and closing of the fluid valve, or the fluid valve is set as a black film opened by laser, which is made of polymer materials or biological materials, and automatically opens when receiving external light, so that the mixed liquid flows into the next chamber. Of course, fluid valves such as solenoid valves commonly used in the prior art can also be selected to achieve the above effects.
[0043] Combination Figures 5 to 7 In the embodiment shown in the figure, the sealing film 80 covers not only the circulation channel 50, but also the lysis chamber 210, the extraction chamber 310, the washing chamber 410 and the fluid channel 110, so as to form a fluid circulation system in the main body of the device. Such a setting can significantly reduce the production cost of the device, the structure is simple, and the sealing film 80 can be set to be transparent. The reading module reads the liquid conditions in each chamber, the fluid channel 110 and the circulation channel 50 from the outside of the sealing film 80, so as to monitor the sample in the overall processing flow.
[0044] In another embodiment, see Figure 8The sample processing device is used for PCR reaction of sample processing, and at least two heating components include a first heating component 41 and a second heating component 42. The temperature of the first temperature control area 11 formed by the first heating component 41 on the heat-conducting base 10 is the first temperature, and the temperature of the second temperature control area 12 formed by the second heating component 42 on the heat-conducting base 10 is the second temperature, wherein the second temperature is lower than the first temperature. The mixed solution is heated in the first temperature control area 11 to denature the DNA or RNA inside, and then renatures in the second temperature control area 12 to complete the amplification purpose, and is read and analyzed by the reading module. Specifically, the first temperature can be set to 95°C, and the second temperature can be set to 65°C. The first heating component 41 can be an electric heating plate or other heating structure, and the temperature is maintained and adjusted by a temperature sensor. The second heating component 42 can be set as a component that can perform heating and cooling operations, such as a high and low temperature cycle device, a semiconductor temperature control device, etc., or other components in the prior art that can achieve temperature maintenance functions, all of which are within the scope of implementation of the present invention.
[0045] Finally, it should be noted that the above description is only the best embodiment of the present invention and does not limit the present invention in any form. Any technician familiar with the art can make many possible changes and simple replacements to the technical solution of the present invention by using the above disclosed practices and technical contents without departing from the scope of the technical solution of the present invention, which all belong to the scope of protection of the technical solution of the present invention.
Claims
1. A temperature control device, characterized in that: include: A heat-conducting base, wherein the heat-conducting base has a closed-loop circulation channel and a fluid inlet connected to the circulation channel, and the fluid inlet is used to introduce a fluid; At least two flow guides, each of which is disposed in the circulation channel and is movable along the closed loop direction of the circulation channel, and an independent fluid receiving space is formed between two adjacent flow guides; At least two heating components are arranged on the heat-conducting base, each of the heating components forms a plurality of independent temperature-controlled areas with different temperatures on the heat-conducting base, and the circulation channel passes through each of the temperature-controlled areas in its closed-loop direction; The driving component is used to drive each of the flow guides to circulate in the circulation channel so that each of the fluid receiving spaces can be placed in each of the temperature control areas.
2. The temperature control device according to claim 1, characterized in that: The driving component can drive each of the flow guides so that each of the fluid receiving spaces is placed in only one corresponding temperature control area, and at this time, there is a corresponding flow guide between two adjacent temperature control areas.
3. The temperature control device according to claim 2, characterized in that: The circulation channel is in the shape of a ring, and two adjacent guide members form a first angle with the center of the circulation channel as the vertex. The fan ring part of the circulation channel in each temperature control area forms a second angle with the center of the circle as the vertex, which is an integer multiple of the first angle.
4. The temperature control device according to claim 3, characterized in that: The guide member is a spherical member, and the diameter of the spherical member is the same as or close to the width of the circulation channel; The driving component comprises a driving plate and a power source for rotating the driving plate. The driving plate abuts against each of the flow guides and drives each of the flow guides to move on the circulation channel when rotating.
5. The temperature control device according to claim 4, characterized in that: The heat-conducting base has a closed fluid channel, and the temperature control device also includes a sealing film, which covers the fluid channel to form the circulation channel, and the guide member squeezes the sealing film from the outside of the sealing film relative to the fluid channel and is placed in the fluid channel.
6. A sample processing device, characterized in that: include: A lysis module, the lysis module comprising a lysis chamber having a sample inlet for introducing the sample; An extraction module, the extraction module comprising an extraction chamber; A washing module, the washing module comprising a washing chamber; An amplification module, comprising the temperature control device according to any one of claims 1 to 5; A reading module, used for reading and analyzing the amplification result signal in the amplification module; The lysis chamber, the extraction chamber, the washing chamber and the circulation channel of the temperature control device are fluidically connected in sequence.
7. The sample processing device according to claim 6, characterized in that: It also includes an equipment body having a fluid channel, the lysis chamber, the extraction chamber, the washing chamber and the circulation channel are arranged on the equipment body along the fluid flow direction of the fluid channel, and the fluid channel is provided with a fluid valve between each chamber.
8. The sample processing device according to claim 7, characterized in that: The fluid valve is a valve composed of a chemical substance that can be opened by heating, or a black membrane that can be opened by laser.
9. The sample processing device according to claim 7, characterized in that: It also includes a sealing film, which covers the lysis chamber, the extraction chamber, the washing chamber, the circulation channel and the fluid channel to form a fluid circulation system in the device body.
10. The sample processing device according to any one of claims 6 to 9, characterized in that: At least two of the heating components include a first heating component and a second heating component. The temperature of the temperature control area formed by the first heating component on the thermally conductive base is a first temperature. The temperature of the temperature control area formed by the second heating component on the thermally conductive base is a second temperature. The second temperature is lower than the first temperature.