Application of pitaya branch polysaccharide in fatigue resistance

Abstract

The invention relates to application of pitaya branch polysaccharide in fatigue resistance, and relates to the technical field of biology. The pitaya branch polysaccharide is an extract obtained from pitaya branches through a water extraction method, has an anti-fatigue effect, improves the fatigue tolerance of an organism, and has great market value of being developed into related products for relieving physical fatigue.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, in particular to application of pitaya branch polysaccharide in anti-fatigue. Background Art

[0002] Studies have shown that 30% to 50% of the people interviewed have symptoms of fatigue. Fatigue is a normal and complex physiological phenomenon. The main causes of this phenomenon include excessive energy consumption, the production and accumulation of metabolites, immune system disorders and oxidative damage. Fatigue usually causes depression and anxiety, reduced learning and work efficiency, cognitive impairment, poor sleep quality and physical dysfunction. In severe cases, long-term fatigue can lead to a variety of complications, such as aging, cancer, multiple sclerosis, human immunodeficiency virus infection and Parkinson's disease. There are also many chronic diseases, such as diabetes, hypertension and kidney disease, which are also related to the occurrence of fatigue.

[0003] At present, there are no chemical drugs for treating fatigue, and most of them are auxiliary treatments. Chemical drugs with anti-fatigue effects are mostly cerebral cortex stimulants, including methylphenidate, piracetam, modafinil, etc., which play an anti-fatigue role by improving the functional activities of the central nervous system. However, most of these anti-fatigue drugs are addictive and have large adverse drug reactions, which limits their clinical application. Therefore, finding safer and more effective fatigue-relieving substances has become a hot topic in the field of anti-fatigue research in recent years. Summary of the invention

[0004] In view of the above problems, the present invention provides an application of pitaya branch polysaccharide in anti-fatigue products. The pitaya branch polysaccharide has anti-fatigue effect and improves the body's fatigue tolerance, and has great market value for developing products related to relieving physical fatigue.

[0005] In order to achieve the above-mentioned purpose, the present invention provides an application of pitaya branch polysaccharide in an anti-fatigue product. The pitaya branch polysaccharide is an extract obtained from pitaya branches by water extraction.

[0006] In one embodiment, the application includes using the pitaya branch polysaccharide to prepare an anti-fatigue product; the product includes a medicine or a food.

[0007] In one embodiment, the pitaya branch polysaccharide is a polysaccharide from red pitaya branches.

[0008] In one embodiment, the food comprises health food.

[0009] In one embodiment, the health food comprises a health drink.

[0010] In one embodiment, the medicine further includes pharmaceutically acceptable excipients; the food further includes food additives.

[0011] In one embodiment, the water extraction method comprises the following steps: soaking pitaya branches in a solvent, removing the solvent to obtain pretreated pitaya branches, adding water, extracting at high temperature and high pressure, taking the supernatant, concentrating to obtain a concentrated solution, adding ethanol, precipitating with alcohol, centrifuging, taking the precipitate, dissolving, removing impurities, dialyzing, and freeze-drying to obtain an extract.

[0012] The water extraction method has the advantages of simple preparation process, few steps, high production efficiency, low cost, safe and convenient operation.

[0013] In one embodiment, the solvent comprises at least one of petroleum ether and ethanol, the soaking time is 3-6 hours, the ratio of the pretreated pitaya branches to the water is 1g: (18-25) mL, the temperature of the high temperature and high pressure extraction is 120-130°C, the time of the high temperature and high pressure extraction is 25-35min, the volume of ethanol in the ethanol adding step is 4-7 times that of the concentrated solution, and the alcohol precipitation time is 20-30h.

[0014] In one embodiment, the dosage form of the drug includes an injectable preparation or an oral preparation.

[0015] In one embodiment, the oral preparation includes tablets, capsules, powders, pills or oral liquid preparations.

[0016] In one embodiment, the application includes oral administration or injection of the drug, or oral administration of the food, and the dosage of the pitaya branch polysaccharide per kilogram of body weight is ≤500 mg.

[0017] In one embodiment, the dosage of the pitaya branch polysaccharide per kilogram of body weight is ≤450 mg.

[0018] In one embodiment, the dosage of the pitaya branch polysaccharide per kilogram of body weight is less than 450 mg.

[0019] In one embodiment, the dosage of the pitaya branch polysaccharide per kilogram of body weight is ≤150 mg.

[0020] The present invention also provides a product for resisting fatigue, which is a medicine or a food, and the medicine or the food comprises the pitaya branch polysaccharide in the application.

[0021] In one embodiment, the medicine further includes pharmaceutically acceptable excipients; the food further includes food additives.

[0022] In one embodiment, the auxiliary material includes at least one of an excipient, a lubricant, an antioxidant, a preservative, a binder, a filler, or a thickener.

[0023] In one embodiment, the product achieves anti-fatigue by reducing serum urea nitrogen levels and increasing liver glycogen and / or muscle glycogen content.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention discloses an application of a dragon fruit branch polysaccharide in anti-fatigue. The dragon fruit branch polysaccharide is taken from dragon fruit branches. Not only is the raw material easy to obtain, cheap, and low in cost, but the raw material itself has no toxic side effects, and the prepared dragon fruit branch polysaccharide can be used safely for a long time. Through mouse experiments, it was found that the dragon fruit branch polysaccharide has an obvious effect, can significantly prolong the weight-bearing swimming time of mice, reduce the serum urea nitrogen level of mice, and increase the liver glycogen and / or muscle glycogen content of mice, showing that the dragon fruit branch polysaccharide has an anti-fatigue effect and improves the body's fatigue tolerance, and has great market value for developing products related to relieving physical fatigue. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The figure shows the effect of different doses of pitaya branch polysaccharide on the weight-bearing swimming time of mice, among which, the pitaya branch polysaccharide dose groups were compared with the blank negative control group *P<0.05, **P<0.01;

[0027] Figure 2 The figure shows the effect of different doses of pitaya branch polysaccharide on the serum urea nitrogen level of mice, among which, pitaya branch polysaccharide dose groups were compared with the blank negative control group *P<0.05, **P<0.01;

[0028] Figure 3 The figure shows the effect of different doses of pitaya branch polysaccharide on the liver glycogen level of mice, among which, pitaya branch polysaccharide dose groups were compared with the blank negative control group *P<0.05, **P<0.01;

[0029] Figure 4 The figure shows the effect of different doses of pitaya branch polysaccharide on the muscle glycogen level of mice, among which, compared with the blank negative control group, *P<0.05, **P<0.01 of each dose group of pitaya branch polysaccharide. DETAILED DESCRIPTION

[0030] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0032] source:

[0033] Unless otherwise specified, the reagents, materials, and equipment used in this example are all commercially available; the experimental methods are all conventional experimental methods in the art unless otherwise specified.

[0034] Example 1

[0035] Extraction of polysaccharides from pitaya branches.

[0036] The polysaccharide from the pitaya branches is extracted by water extraction, and the specific steps are as follows:

[0037] 1. Cut the red pitaya branches into slices and dry them under reduced pressure until the quality is constant;

[0038] 2. Soak and extract with petroleum ether and 95% ethanol for 4 hours to remove fat-soluble components such as pigments and small molecular components, and evaporate the solvent to obtain the pretreated sample;

[0039] 3. Accurately weigh the pretreated sample, add water at a solid-liquid ratio of 1:20, place it in a pressure cooker extraction device, and extract at 121°C for 30 minutes;

[0040] 4. Concentrate the supernatant to 1 / 5 of the original volume, add 5 times the volume of anhydrous ethanol, precipitate for 24 hours, and centrifuge to obtain the precipitate;

[0041] 5. Dissolve the precipitate with an appropriate amount of deionized water to remove insoluble proteins, and dialyze with deionized water to remove small molecule impurities and freeze-dried polysaccharide components.

[0042] Example 2

[0043] Effects of polysaccharides from pitaya branches on weighted swimming in mice.

[0044] 1. Inspection principle.

[0045] The improvement of exercise endurance is the most direct manifestation of enhanced anti-fatigue ability, and the length of swimming time can reflect the degree of fatigue.

[0046] 2. Experimental methods.

[0047] (1) Experimental animals: SPF-grade KM mice were purchased from the Guangdong Provincial Medical Experimental Animal Center, adult males, weighing 18 - 22 g, with the license number SCXK(Guangdong)2022 - 0002.

[0048] (2) Dose grouping and administration time of the test samples: The experiment was set up with three dose groups of pitaya branch polysaccharides at low, medium, and high doses (50 mg / kg, 150 mg / kg, and 450 mg / kg respectively) and a blank negative control group, with 8 mice in each group. The test samples were administered by gavage once a day, and the blank negative control group was gavaged with tap water. The administration time of the test samples was 30 days.

[0049] (3) Experimental procedures: 30 minutes after the last administration of the test samples, mice with a 5% body weight lead sheet attached to the tail root were placed in a swimming tank to swim. The water depth was not less than 30 cm, and the water temperature was 25°C ± 1.0°C. When the mouse sank into the water and could not surface for 3 - 4 seconds, it was judged to be in a state of exhaustive swimming, and the time from the start of swimming to exhaustion of the mouse was recorded, that is, the weight-bearing swimming time of the mouse. After swimming, the mouse's fur was dried and it was put back.

[0050] (4) Statistical analysis: One-way analysis of variance of the single-factor test was performed using SPSS12 software. When P < 0.05, it indicated that the difference was significant.

[0051] 3. Experimental results.

[0052] As Figure 1 shown, the weight-bearing swimming time of mice gavaged with pitaya branch polysaccharides, regardless of the low, medium, or high dose group, was significantly longer than that of the blank control group mice.

[0053] Example 3

[0054] Effect of pitaya branch polysaccharides on the serum urea nitrogen level of mice.

[0055] 1. Test principle.

[0056] During strenuous exercise of the body, the accelerated decomposition of proteins leads to an increase in the level of urea nitrogen, the end product of its metabolism. Therefore, urea nitrogen can be used as a sensitive indicator of body fatigue. Prolonged strenuous exercise causes a sharp increase in the body's demand for oxygen and energy, weakens the aerobic oxidation pathway of sugar, and enhances anaerobic glycolysis and protein metabolism, producing metabolic products such as lactic acid and urea nitrogen, thus leading to fatigue. Therefore, lactic acid and urea nitrogen can reflect the degree of body fatigue.

[0057] 2. Experimental methods.

[0058] (1) Same as Example 1, the test sample was administered for 30 days. 30 minutes after the last administration of the test sample, the mice were allowed to swim without weight in water at a temperature of 30°C for 90 minutes, and blood was collected after resting for 60 minutes. The eyeballs of the mice were removed and 0.5 mL of whole blood (without anticoagulant) was collected. The blood was placed in a 4°C refrigerator for about 3 hours. After the blood coagulated, it was centrifuged at 2000prn / min for 15 minutes, and the serum was collected for later use. The urea content (enzymatic method) detection kit of Quanzhou Ruixin Biotechnology Co., Ltd. was used, and the operating method of the attached instruction manual was followed to detect the serum urea nitrogen level of each group of mice.

[0059] (2) Statistical analysis: SPSS12 software was used to perform one-way analysis of variance. When P < 0.05, the difference was considered significant.

[0060] 3. Experimental results.

[0061] like Figure 2 As shown, the serum urea nitrogen levels of mice gavaged with pitaya branch polysaccharide were significantly lower than those of mice in the blank control group, regardless of the low, medium or high dose groups.

[0062] Example 4

[0063] Effects of pitaya branch polysaccharides on liver glycogen and muscle glycogen levels in mice.

[0064] 1. Inspection principle.

[0065] The body's energy is mainly stored in the form of glycogen. When the body is exercising continuously or at a high intensity, in order to maintain blood sugar balance, the liver glycogen and muscle glycogen in the body will break down for energy. The amount of glycogen reserves in the body is positively correlated with the body's resistance to fatigue.

[0066] 2. Experimental method.

[0067] (1) The same as in Example 1, except that the test samples were administered for 30 days. The animals were killed 30 minutes after the last administration of the test samples. The liver and quadriceps femoris were washed with saline and then dried with filter paper. The glycogen content assay kit of Quanzhou Ruixin Biotechnology Co., Ltd. was used to detect the liver glycogen and muscle glycogen levels of each group of mice according to the operating method in the attached instructions.

[0068] (2) Statistical analysis: SPSS12 software was used to perform one-way analysis of variance. When P < 0.05, the difference was considered significant.

[0069] 3. Experimental results.

[0070] like Figure 3 , Figure 4As shown, the liver glycogen levels of mice gavaged with pitaya branch polysaccharide were significantly higher than those of mice in the blank control group, regardless of the low, medium or high dose groups; while the muscle glycogen levels of mice were significantly higher than those of mice in the blank control group only in the low and medium dose groups.

[0071] Result determination

[0072] According to the relevant provisions of the 2003 edition of the "Technical Specifications for Inspection and Evaluation of Health Foods", the results of the weighted swimming test were positive, and serum urea and liver glycogen / muscle glycogen were all positive. It can be determined that pitaya branch polysaccharides, at appropriate doses, have the function of relieving physical fatigue.

[0073] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0074] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An application of pitaya branch polysaccharide in anti-fatigue products, characterized in that: The pitaya branch polysaccharide is an extract obtained from the pitaya branch by water extraction.

2. The use according to claim 1, characterized in that: The application includes preparing anti-fatigue products using the pitaya branch polysaccharide; the products include medicines or foods.

3. The use according to claim 2, characterized in that: The medicine also includes pharmaceutically acceptable excipients; the food also includes food additives.

4. The use according to claim 1, characterized in that: The water extraction method comprises the following steps: soaking pitaya branches with a solvent, removing the solvent to obtain pretreated pitaya branches, adding water, extracting at high temperature and high pressure, taking the supernatant, concentrating to obtain a concentrated solution, adding ethanol, precipitating with alcohol, centrifuging, taking the precipitate, dissolving, removing impurities, dialysis, and freeze-drying to obtain an extract.

5. The use according to claim 1, characterized in that: The dosage form of the drug includes an injection preparation or an oral preparation.

6. The use according to claim 5, characterized in that: The oral preparations include tablets, capsules, powders, drop pills or oral liquid preparations.

7. The use according to claim 2, characterized in that: The application includes oral administration or injection of the drug, or oral administration of the food, and the dosage of the pitaya branch polysaccharide per kilogram of body weight is ≤500 mg.

8. A product for anti-fatigue, characterized in that: The product is a medicine or food, and the medicine or food comprises the pitaya branch polysaccharide used in any one of claims 1 to 7.

9. The product according to claim 8, characterized in that The medicine also includes pharmaceutically acceptable excipients; the food also includes food additives.

10. The product according to claim 8, characterized in that The product achieves anti-fatigue by reducing the level of serum urea nitrogen and increasing the content of liver glycogen and / or muscle glycogen.

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