Drug residue detection device and drug residue detection method

Abstract

The present invention discloses a drug residue detection device and a drug residue detection method, which relate to the technical field of pre-prepared food raw material detection, and include a raw material crusher and a mixing box, wherein a hydrolysis tube and an enzyme reagent container are provided in the mixing box. A quantitative tissue fluid is intercepted through the liquid inlet of the hydrolysis tube, and then a quantitative enzyme reagent is introduced after the liquid inlet is hidden, and the avoidance chamber in the hydrolysis tube is opened with the help of the pressure of the enzyme reagent to provide a holding space for the enzyme reagent. A quantitative tissue fluid and a quantitative enzyme reagent are respectively intercepted through the up and down movement of the hydrolysis tube, so that the amount of the enzyme reagent can be adapted to the amount of the tissue fluid. After the enzyme reagent and the tissue fluid are mixed in the hydrolysis tube, the hydrolyzed solution can be detected by the existing spectral technology, thereby obtaining an accurate concentration of the raw material drug residue.

Description

Technical Field

[0001] The invention relates to the technical field of pre-prepared food raw material detection, and in particular to a drug residue detection device and a drug residue detection method. Background Art

[0002] The production process of pre-prepared dishes must go through the cleaning and drug residue detection links. The drug residue detection of pre-prepared dish raw materials is usually detected by spectral technology. Reference can be made to the rapid detection device for pesticide residues in cold fresh pre-prepared dishes disclosed in Chinese patent CN221405424U. Specifically, the raw material sample is organizationally crushed to extract the pesticides that may remain in the raw material matrix, and the crushed tissue fluid is filtered, and then mixed with enzyme reagents (acetylcholine, cholinesterase and color developer) for hydrolysis reaction. Since pesticides have an inhibitory effect on the normal function of cholinesterase, its inhibition rate is related to the concentration of pesticides. Under normal circumstances, the enzyme catalyzes the hydrolysis of nerve conduction metabolites (acetylcholine), and its hydrolysis products react with the color developer to produce yellow substances. Finally, the raw material sample solution after the hydrolysis reaction is placed in a vessel and measured using a spectrophotometer. The inhibition rate is calculated based on the change in absorbance over time. The inhibition rate can be used to determine whether the raw material sample contains pesticide residues.

[0003] At present, the ratio of tissue fluid to enzyme reagent is usually manually measured by quantitatively aspirating through a Pasteur pipette. This ratio is inefficient and will reduce the efficiency of drug residue detection. Therefore, it needs to be improved. Summary of the invention

[0004] One of the purposes of the present invention is to solve the problem that the efficiency of drug residue detection is reduced by artificially mixing tissue fluid and enzyme reagent in the prior art.

[0005] A second object of the present invention is to provide a method for detecting drug residues.

[0006] In order to achieve one of the above-mentioned purposes, the present invention adopts the following technical scheme: a drug residue detection device, comprising a raw material grinder and a mixing box, the mixing box is arranged under the raw material grinder, a raw material inlet is provided on the raw material grinder, and a tissue fluid discharge outlet extending into the mixing box liquid pool is provided under the raw material grinder.

[0007] The mixing box is equipped with a hydrolysis tube and an enzyme reagent container, a lifting cavity is arranged under the liquid pool, the hydrolysis tube is slidably and sealedly connected to the lifting cavity, and the upper end of the hydrolysis tube is exposed in the liquid pool.

[0008] The hydrolysis tube is provided with a hydrolysis chamber and an avoidance chamber which are in communication. A pressure plate is installed in a sliding seal at the bottom of the avoidance chamber. The pressure plate is located above the hydrolysis chamber. A boss is provided on the bottom side of the avoidance chamber. The pressure plate is overlapped on the boss.

[0009] A liquid inlet and a liquid outlet are arranged at the side end of the hydrolysis chamber, wherein the liquid inlet is located above the liquid outlet, the liquid inlet is connected to the liquid pool, and the liquid outlet is sealed by the inner wall surface of the lifting chamber.

[0010] The enzyme reagent container is equipped with a connected nozzle and a liquid pump, the nozzle is located on the side end surface of the lifting chamber, the nozzle and the liquid outlet are staggered, and the liquid outlet is located above the nozzle.

[0011] A liquid outlet nozzle is installed at the bottom of the mixing box, a solution port is arranged at the side end of the liquid outlet nozzle, the solution port is located at the side end surface of the lifting chamber, and the solution port is located below the nozzle.

[0012] The mixing box also has a lifting cylinder, a connecting rod is fixed to the telescopic end of the lifting cylinder, and the connecting rod is connected to the hydrolysis tube. The lifting cylinder drives the hydrolysis tube to move up and down to receive a quantitative tissue fluid and a quantitative enzyme reagent.

[0013] In the above technical scheme, when the embodiment of the present invention is used, the raw material sample is placed in the raw material inlet, and the raw material sample is crushed by the raw material crusher, so that the raw material tissue fluid obtained after crushing is discharged from the tissue fluid discharge port into the liquid pool of the mixing box.

[0014] The tissue fluid then enters the hydrolysis chamber through the liquid inlet of the hydrolysis tube, so that the tissue fluid fills the hydrolysis chamber.

[0015] Then, the connecting rod and the hydrolysis tube connected to the connecting rod are driven downward by the lifting cylinder, so that the liquid inlet of the hydrolysis tube is hidden in the lifting chamber for sealing, and the liquid outlet of the hydrolysis tube is connected to the nozzle of the enzyme reagent container. At this time, the liquid pump of the enzyme reagent container is started to send the enzyme reagent from the nozzle into the hydrolysis chamber for mixing and hydrolysis.

[0016] When the enzyme reagent enters the hydrolysis chamber, the pressure plate on the hydrolysis chamber is pressed upward, so that the tissue fluid in the hydrolysis chamber enters the avoidance chamber, providing a holding space for the enzyme reagent.

[0017] When the enzyme reagent is mixed with the tissue fluid and hydrolyzed into a solution, the hydrolysis tube is driven downward again by the lifting cylinder, so that the liquid outlet of the hydrolysis tube is connected with the solution outlet of the liquid outlet nozzle, and then the solution in the hydrolysis chamber is discharged from the liquid outlet nozzle.

[0018] Finally, the absorbance of the solution discharged from the outlet nozzle is detected by spectral technology to obtain the drug residue concentration of the raw material.

[0019] The beneficial effects of the present invention are:

[0020] First, the present invention intercepts a quantitative amount of tissue fluid in the liquid pool through the liquid inlet of the hydrolysis tube, and then hides the liquid inlet of the hydrolysis tube to keep excess tissue fluid in the liquid pool, and then feeds the enzyme reagent into the hydrolysis tube, and uses the pressure of the enzyme reagent to open the escape chamber in the hydrolysis tube to provide a storage space for the enzyme reagent. The tissue fluid intercepted by the hydrolysis tube is quantitative, and accordingly, the quantitative enzyme reagent fed in can be adapted to the amount of tissue fluid, so that the enzyme reagent and the tissue fluid are mixed and hydrolyzed in the hydrolysis tube to form a solution, which can be detected by existing spectral technology to obtain an accurate concentration of the residual drug substance.

[0021] Second, the hydrolysis tube of the present invention implements the interception of quantitative tissue fluid, the mixed hydrolysis of quantitative enzyme reagent and quantitative tissue fluid, and the discharge of raw material solution by moving up and down. This method is simple and efficient, and the hydrolyzed solution can be detected by existing spectral technology to obtain an accurate residual concentration of the raw material drug. The quantitative tissue fluid and the quantitative enzyme reagent are intercepted respectively by the up and down movement of the hydrolysis tube, so that the amount of enzyme reagent can be adapted to the amount of tissue fluid.

[0022] Further, in an embodiment of the present invention, the side end of the mixing box is connected to the support leg, and the bottom of the support leg is connected to the support plate.

[0023] Furthermore, in an embodiment of the present invention, a filtering structure is provided in the tissue fluid outlet to filter raw material impurities.

[0024] Furthermore, in an embodiment of the present invention, the hydrolysis tube and the lifting chamber are sealed by a sealing ring, and the pressure plate and the avoidance chamber are sealed by a sealing ring.

[0025] Furthermore, in an embodiment of the present invention, an ultrasonic generator is installed on the pressing plate.

[0026] Furthermore, in an embodiment of the present invention, a heating resistance wire is embedded in the hydrolysis tube.

[0027] Furthermore, in an embodiment of the present invention, a curved and fitted recoil elastic sheet is provided in the nozzle, and the recoil elastic sheet is curved in the direction of the lifting chamber.

[0028] To achieve the second of the above objects, the present invention adopts the following technical solution: a drug residue detection method, the drug residue detection method is based on the drug residue detection device described in one of the above invention objects, and the drug residue detection method comprises the following steps:

[0029] The raw material sample is placed in the raw material inlet, and the raw material sample is crushed by the raw material crusher, so that the raw material tissue fluid obtained after the crushing is discharged from the tissue fluid discharge port into the liquid pool of the mixing box.

[0030] The tissue fluid then enters the hydrolysis chamber through the liquid inlet of the hydrolysis tube, so that the tissue fluid fills the hydrolysis chamber.

[0031] Then, the connecting rod and the hydrolysis tube connected to the connecting rod are driven downward by the lifting cylinder, so that the liquid inlet of the hydrolysis tube is hidden in the lifting chamber for sealing, and the liquid outlet of the hydrolysis tube is connected to the nozzle of the enzyme reagent container. At this time, the liquid pump of the enzyme reagent container is started to send the enzyme reagent from the nozzle into the hydrolysis chamber for mixing and hydrolysis.

[0032] When the enzyme reagent enters the hydrolysis chamber, the pressure plate on the hydrolysis chamber is pressed upward, so that the tissue fluid in the hydrolysis chamber enters the avoidance chamber, providing a holding space for the enzyme reagent.

[0033] When the enzyme reagent is mixed with the tissue fluid and hydrolyzed into a solution, the hydrolysis tube is driven downward again by the lifting cylinder, so that the liquid outlet of the hydrolysis tube is connected with the solution outlet of the liquid outlet nozzle, and then the solution in the hydrolysis chamber is discharged from the liquid outlet nozzle.

[0034] Finally, the absorbance of the solution discharged from the outlet nozzle is detected by spectral technology to obtain the drug residue concentration of the raw material.

[0035] Furthermore, in an embodiment of the present invention, in the above steps, during the process of discharging the solution, the pressing plate is reset under the action of its own gravity as the liquid level of the hydrolysis chamber drops.

[0036] When the solution in the hydrolysis chamber is completely discharged, the hydrolysis tube is driven to reset by the lifting cylinder so that the liquid inlet of the hydrolysis tube is connected to the liquid pool again.

[0037] Furthermore, in an embodiment of the present invention, in the above steps, after the enzyme reagent enters the hydrolysis chamber, ultrasonic waves are emitted by an ultrasonic generator on the pressing plate to generate a cavitation effect when the enzyme reagent and the tissue fluid are mixed, thereby promoting the mixing of the enzyme reagent and the tissue fluid. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 Schematic diagram of a drug residue detection device according to an embodiment of the present invention.

[0039] Figure 2 It is a schematic diagram of the partial structure of the drug residue detection device according to an embodiment of the present invention.

[0040] Figure 3 Schematic diagram of the first flow effect of the raw material tissue fluid in the drug residue detection device according to an embodiment of the present invention.

[0041] Figure 4 Schematic diagram of the second flow effect of the raw tissue fluid in the drug residue detection device according to an embodiment of the present invention.

[0042] Figure 5 Schematic diagram of the first action effect of the drug residue detection device according to an embodiment of the present invention.

[0043] Figure 6 Schematic diagram of the second action effect of the drug residue detection device according to an embodiment of the present invention.

[0044] Figure 7 Schematic diagram of the third action effect of the drug residue detection device according to an embodiment of the present invention.

[0045] 10. Raw material crusher, 11. Raw material inlet, 12. Tissue fluid outlet;

[0046] 20. mixing box, 21. liquid pool, 22. lifting chamber, 23. liquid outlet, 24. solution outlet, 25. supporting legs;

[0047] 30. hydrolysis tube, 31. hydrolysis chamber, 32. avoidance chamber, 33. pressing plate, 34. liquid inlet, 35. liquid outlet, 36. ultrasonic generator, 37. heating resistance wire;

[0048] 40. enzyme reagent container, 41. nozzle, 42. liquid pump, 43. recoil elastic sheet;

[0049] 50. Lifting cylinder, 51. Connecting rod. DETAILED DESCRIPTION

[0050] In order to make the purpose and technical solution of the present invention clearly and completely described, and the advantages more clearly understood, the embodiments of the present invention are further described in detail with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are part of the embodiments of the present invention, rather than all of the embodiments, and are only used to explain the embodiments of the present invention, and are not used to limit the embodiments of the present invention. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0051] In the description of the present invention, it should be noted that the terms "center", "middle", "upper", "lower", "left", "right", "inner", "outer", "top", "bottom", "side", "vertical", "horizontal" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "one", "first", "second", "third", "fourth", "fifth", "sixth" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0052] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0053] For the purpose of simplicity and explanation, the principle of the embodiment is mainly described by reference to example. In the following description, many details are proposed to provide a thorough understanding of the embodiment. However, it is obvious. For those of ordinary skill in the art, these embodiments may not be limited to these details in practice. In some instances, known drug residue detection methods and structures are not described in detail to avoid making these embodiments unnecessarily difficult to understand. In addition, all embodiments can be used in combination with each other. Example 1

[0054] It should be noted that the drawings in the specification are the contents of the specification. The structural shapes, connection relationships, matching relationships, and positional relationships that can be obtained without any doubt in the drawings in the specification should be understood as the contents of the specification.

[0055] A drug residue detection device, such as Figure 1 As shown, it includes a raw material grinder 10 and a mixing box 20. The mixing box 20 is arranged under the raw material grinder 10. A raw material inlet 11 is arranged on the raw material grinder 10, and a tissue fluid outlet 12 extending into a liquid pool 21 of the mixing box 20 is arranged under the raw material grinder 10.

[0056] The raw material crusher 10 is a common mechanical structure in the art, and its operating principle and effects can be understood and directly applied by those skilled in the art without creative work, so the present invention will not explain it in detail.

[0057] like Figure 2 As shown, the mixing box 20 is equipped with a hydrolysis tube 30 and an enzyme reagent container 40 , a lifting chamber 22 is arranged under the liquid pool 21 , the hydrolysis tube 30 is slidably and sealingly connected to the lifting chamber 22 , and the upper end of the hydrolysis tube 30 is exposed in the liquid pool 21 .

[0058] A hydrolysis chamber 31 and an escape chamber 32 are provided in the hydrolysis tube 30. A pressure plate 33 is installed in a sliding seal at the bottom of the escape chamber 32. The pressure plate 33 is located above the hydrolysis chamber 31. A boss is provided on the bottom side of the escape chamber 32, and the pressure plate 33 is overlapped on the boss.

[0059] A liquid inlet 34 and a liquid outlet 35 are provided at the side end of the hydrolysis chamber 31 . The liquid inlet 34 is located above the liquid outlet 35 . The liquid inlet 34 is connected to the liquid pool 21 . The liquid outlet 35 is sealed by the inner wall surface of the lifting chamber 22 .

[0060] The enzyme reagent container 40 is provided with a nozzle 41 and a liquid pump 42 connected to each other. The nozzle 41 is located at the side end surface of the lifting chamber 22 . The nozzle 41 and the liquid outlet 35 are staggered, and the liquid outlet 35 is located above the nozzle 41 .

[0061] A liquid outlet nozzle 23 is installed at the bottom of the mixing box 20 , and a solution outlet 24 is arranged at the side end of the liquid outlet nozzle 23 . The solution outlet 24 is located at the side end surface of the lifting chamber 22 , and the solution outlet 24 is located below the nozzle 41 .

[0062] The mixing box 20 also has a lifting cylinder 50, and a connecting rod 51 is fixed to the telescopic end of the lifting cylinder 50. The connecting rod 51 is connected to the hydrolysis tube 30. The lifting cylinder 50 drives the hydrolysis tube 30 to move up and down to receive a quantitative tissue fluid and a quantitative enzyme reagent.

[0063] The lifting cylinder 50 may be a hydraulic cylinder, a pneumatic cylinder, an electric cylinder, or other lifting machinery commonly used in the prior art, and the present invention is not limited thereto.

[0064] Implementation steps: put the raw material sample into the raw material inlet 11, grind the raw material sample through the raw material grinder 10, and discharge the raw material tissue fluid obtained after grinding from the tissue fluid discharge port 12 into the liquid pool 21 of the mixing box 20.

[0065] like Figure 3 , Figure 4 As shown, the tissue fluid then enters the hydrolysis chamber 31 through the liquid inlet 34 of the hydrolysis tube 30 , so that the tissue fluid fills the hydrolysis chamber 31 .

[0066] Then, if Figure 5 As shown, the connecting rod 51 and the hydrolysis tube 30 connected to the connecting rod 51 are driven downward by the lifting cylinder 50, so that the liquid inlet 34 of the hydrolysis tube 30 is hidden in the lifting chamber 22 for sealing, and the liquid outlet 35 of the hydrolysis tube 30 is connected to the nozzle 41 of the enzyme reagent container 40. At this time, the liquid pump 42 of the enzyme reagent container 40 is started to send the enzyme reagent from the nozzle 41 into the hydrolysis chamber 31 for mixing and hydrolysis.

[0067] like Figure 6 As shown, during the process of the enzyme reagent entering the hydrolysis chamber 31, the pressure plate 33 on the hydrolysis chamber 31 is pressed upward, so that the tissue fluid in the hydrolysis chamber 31 enters the avoidance chamber 32, providing a holding space for the enzyme reagent.

[0068] like Figure 7As shown, after the enzyme reagent is mixed with the tissue fluid and hydrolyzed into a solution, the hydrolysis tube 30 is driven downward again by the lifting cylinder 50, so that the liquid outlet 35 of the hydrolysis tube 30 is connected with the solution outlet 24 of the liquid outlet nozzle 23, and then the solution in the hydrolysis chamber 31 is discharged from the liquid outlet nozzle 23.

[0069] Finally, the absorbance of the solution discharged from the liquid outlet 23 is detected by spectral technology to obtain the drug residue concentration of the raw material.

[0070] It should be noted that the improvement of the present invention is that a certain amount of tissue fluid can be intercepted and mixed with the enzyme reagent. No improvement is made to the spectral technology, so those skilled in the art can detect the drug residue concentration of the raw material based on the existing spectral technology.

[0071] It should be noted that the quantitative tissue fluid depends on the specific maximum amount that the hydrolysis tube 30 can accommodate, and the limit refers to the amount that the hydrolysis chamber 31, the liquid inlet 34 and the liquid outlet 35 can accommodate when the pressure plate does not move. The quantitative enzyme reagent depends on the quantitative tissue fluid. Referring to the "Rapid Test Method for Organic Phosphorus Pesticide Residues in Vegetables - Enzyme Inhibition Rate Method (Spectrophotometry)" disclosed by Everlab Cloud Laboratory in the prior art, spectrophotometry is a spectral technology, which specifically discloses:

[0072] Add 2.5 mL of buffer, 0.1 mL of enzyme solution, and 0.1 mL of colorimetric agent (enzyme reagent) to the test tube, add 2.5 mL of sample extract (tissue fluid) to the test tube, and record the change in absorbance ΔA after 3 min of reaction. t (The change in absorbance of the sample solution after 3 min of reaction).

[0073] It can be seen from the above existing experimental schemes that those skilled in the art can understand the specific values ​​of the quantitative enzyme reagent and the quantitative tissue fluid without any creative work.

[0074] One of the advantages of the present invention is that the present invention intercepts a quantitative amount of tissue fluid in the liquid pool 21 through the liquid inlet 34 of the hydrolysis tube 30, and then hides the liquid inlet 34 of the hydrolysis tube 30 to keep the excess tissue fluid in the liquid pool 21, and then the enzyme reagent is sent into the hydrolysis tube 30, and the avoidance chamber 32 in the hydrolysis tube 30 is opened by the pressure of the enzyme reagent to provide a storage space for the enzyme reagent. The tissue fluid intercepted by the hydrolysis tube 30 is quantitative, and accordingly, the quantitative enzyme reagent sent in can be adapted to the amount of tissue fluid.

[0075] The second advantage of the present invention is that the hydrolysis tube 30 of the present invention intercepts a certain amount of tissue fluid and a certain amount of enzyme reagent by moving up and down, so that the amount of enzyme reagent can be adapted to the amount of tissue fluid, so that the hydrolyzed solution can be detected by existing spectral technology, thereby obtaining an accurate concentration of the residual raw material drug.

[0076] Specifically, Figure 1 As shown, the side end of the mixing box 20 is connected to the support leg 25, and the bottom of the support leg 25 is connected to the support plate.

[0077] Specifically, a filtering structure (not shown) is provided in the tissue fluid outlet 12 to filter raw material impurities.

[0078] Pigments, organic acids, phenols and other impurities are common in tissue fluids. The detection accuracy of these impurities is affected, so a filtering structure is provided in the prior art to filter out the impurities. For details, please refer to "A magnetic carbon nanotube for use in pesticide residue detection" disclosed in CN104084127B.

[0079] Specifically, Figure 2 As shown, the hydrolysis tube 30 and the lifting chamber 22 are sealed by a sealing ring, and the pressure plate 33 and the avoidance chamber 32 are sealed by a sealing ring.

[0080] Specifically, Figure 2 As shown, an ultrasonic generator 36 is installed on the pressing plate 33. A heating resistance wire 37 is embedded in the hydrolysis tube 30.

[0081] The ultrasonic generator 36 and the heating resistor wire 37 are both common technical solutions in the prior art. Both have complete implementation plans and are not difficult to understand and implement for those skilled in the art. Therefore, the present invention does not explain their principles and structures in detail.

[0082] The ultrasonic generator 36 on the pressing plate 33 of the present invention emits ultrasonic waves to generate cavitation effect when the enzyme reagent and the tissue fluid are mixed, thereby promoting the mixing of the enzyme reagent and the tissue fluid. The mixing temperature of the enzyme reagent and the tissue fluid can be adjusted by the action of the heating resistor 37 so as to better mix them.

[0083] The third advantage of the present invention is that the pressing plate 33 is a "floating" upper and lower structure, and an ultrasonic generator 36 is arranged on it, which can perform cavitation effect on the tissue fluid in the hydrolysis chamber 31 before hydrolysis to prevent possible condensation of the tissue fluid.

[0084] Specifically, Figure 2 , Figure 6 As shown, a curved and fitted recoil elastic sheet 43 is provided in the nozzle 41 , and the recoil elastic sheet 43 is curved in the direction of the lifting chamber 22 .

[0085] The pressure of the enzyme reagent delivered by the liquid pump 42 stretches the recoil elastic sheet 43 to separate the recoil elastic sheets 43 that are in contact with each other. After separation, a channel is formed, so that the enzyme reagent enters the hydrolysis chamber 31 through the channel.

[0086] The fourth advantage of the present invention is that when the tissue fluid or enzyme reagent in the hydrolysis chamber 31 squeezes the closed recoil elastic sheet 43 through the liquid outlet 35, the recoil elastic sheet 43 bends in the direction of the lifting chamber 22, so the squeezing force will intensify the adhesion force between the adhesion surfaces of the recoil elastic sheets 43, so that the recoil elastic sheets 43 cannot be separated, which is helpful to avoid the loss of tissue fluid or enzyme reagent in the hydrolysis chamber 31. Example 2

[0087] A drug residue detection method is provided. The drug residue detection method is based on the drug residue detection device in the above-mentioned embodiment 1. The drug residue detection method comprises the following steps:

[0088] The raw material sample is put into the raw material inlet 11 , and the raw material sample is crushed by the raw material crusher 10 , so that the raw material tissue fluid obtained after the crushing is discharged from the tissue fluid discharge port 12 into the liquid pool 21 of the mixing box 20 .

[0089] The tissue fluid then enters the hydrolysis chamber 31 through the liquid inlet 34 of the hydrolysis tube 30 , so that the tissue fluid fills the hydrolysis chamber 31 .

[0090] Then, the connecting rod 51 and the hydrolysis tube 30 connected to the connecting rod 51 are driven downward by the lifting cylinder 50, so that the liquid inlet 34 of the hydrolysis tube 30 is hidden in the lifting chamber 22 for sealing, and the liquid outlet 35 of the hydrolysis tube 30 is connected to the nozzle 41 of the enzyme reagent container 40. At this time, the liquid pump 42 of the enzyme reagent container 40 is started to send the enzyme reagent from the nozzle 41 into the hydrolysis chamber 31 for mixing and hydrolysis.

[0091] When the enzyme reagent enters the hydrolysis chamber 31 , the pressure plate 33 on the hydrolysis chamber 31 is pressed upward, so that the tissue fluid in the hydrolysis chamber 31 enters the avoidance chamber 32 , providing a storage space for the enzyme reagent.

[0092] When the enzyme reagent is mixed with the tissue fluid and hydrolyzed into a solution, the lifting cylinder 50 drives the hydrolysis tube 30 downward again, so that the liquid outlet 35 of the hydrolysis tube 30 communicates with the solution outlet 24 of the liquid outlet nozzle 23, and then the solution in the hydrolysis chamber 31 is discharged from the liquid outlet nozzle 23.

[0093] Finally, the absorbance of the solution discharged from the liquid outlet 23 is detected by spectral technology to obtain the drug residue concentration of the raw material.

[0094] The present invention intercepts a quantitative amount of tissue fluid through the liquid inlet 34 of the hydrolysis tube 30, and then after hiding the liquid inlet 34, a quantitative amount of enzyme reagent is fed in, and the avoidance chamber 32 in the hydrolysis tube 30 is opened by the pressure of the enzyme reagent to provide a storage space for the enzyme reagent. Through the up and down movement of the hydrolysis tube 30, a quantitative amount of tissue fluid and a quantitative amount of enzyme reagent are intercepted respectively, so that the amount of enzyme reagent can be adapted to the amount of tissue fluid. When the enzyme reagent and tissue fluid are mixed in the hydrolysis tube 30 and hydrolyzed into a solution, it can be detected by existing spectral technology, thereby obtaining an accurate concentration of the residual drug substance.

[0095] Specifically, in the above steps, during the process of discharging the solution, the pressing plate 33 is reset under the action of its own gravity as the liquid level of the hydrolysis chamber 31 drops.

[0096] After the solution in the hydrolysis chamber 31 is completely discharged, the hydrolysis tube 30 is driven to reset by the lifting cylinder 50 so that the liquid inlet 34 of the hydrolysis tube 30 is connected to the liquid pool 21 again.

[0097] Specifically, in the above steps, after the enzyme reagent enters the hydrolysis chamber 31, ultrasonic waves are emitted by the ultrasonic generator 36 on the pressing plate 33, so that the enzyme reagent and the tissue fluid produce a cavitation effect when mixed, thereby promoting the mixing of the enzyme reagent and the tissue fluid.

[0098] Although the above describes the illustrative specific embodiments of the present invention so that those skilled in the art can understand the present invention, the present invention is not limited to the scope of the specific embodiments. For those of ordinary skill in the art, as long as various changes are within the spirit and scope of the present invention as defined and determined by the attached claims, all inventions and creations using the concepts of the present invention are protected.

Claims

1. A drug residue detection device, comprising a mixing box, wherein a liquid pool is provided in the mixing box; It is characterized in that The mixing box is equipped with a hydrolysis tube and an enzyme reagent container, the hydrolysis tube is slidably and sealedly connected to the lifting chamber under the liquid pool, and the upper end of the hydrolysis tube is exposed in the liquid pool; The hydrolysis tube is provided with a hydrolysis chamber and an escape chamber which are in communication, a pressure plate is installed in a sliding and sealing manner at the bottom of the escape chamber, the pressure plate is located above the hydrolysis chamber, a boss is provided at the bottom side of the escape chamber, and the pressure plate is overlapped on the boss; A liquid inlet and a liquid outlet are provided at the side end of the hydrolysis chamber, and the liquid inlet is connected to the liquid pool; The enzyme reagent container is equipped with a connected nozzle and a liquid pump, the nozzle is located on the side end surface of the lifting chamber, and the nozzle is staggered with the liquid outlet; A liquid outlet nozzle is installed at the bottom of the mixing box, a solution outlet is arranged at the side end of the liquid outlet nozzle, and the solution outlet is located under the nozzle; The mixing box is also provided with a lifting cylinder, which is connected to the hydrolysis tube via a connecting rod. The lifting cylinder drives the hydrolysis tube to move up and down to receive a quantitative tissue fluid and a quantitative enzyme reagent.

2. The drug residue detection device according to claim 1, characterized in that: The side ends of the mixing box are connected to the legs, and the bottoms of the legs are connected to the support plates.

3. The drug residue detection device according to claim 1, characterized in that: The tissue fluid outlet is provided with a filtering structure to filter raw material impurities.

4. The drug residue detection device according to claim 1, characterized in that: The hydrolysis tube and the lifting chamber are sealed by a sealing ring, and the pressure plate and the avoidance chamber are sealed by a sealing ring.

5. The drug residue detection device according to claim 1, characterized in that: An ultrasonic generator is installed on the pressing plate.

6. The drug residue detection device according to claim 5, characterized in that: A heating resistance wire is embedded in the hydrolysis tube.

7. The drug residue detection device according to claim 1, characterized in that: A curved and fitted recoil elastic sheet is arranged in the nozzle, and the recoil elastic sheet is curved in the direction of the lifting chamber.

8. A method for detecting drug residues, characterized in that: The drug residue detection method is based on the drug residue detection device described in any one of claims 1 to 7, and the drug residue detection method comprises the following steps: Put the raw material sample into the raw material inlet, crush the raw material sample by the raw material crusher, and discharge the raw material tissue fluid obtained after crushing from the tissue fluid discharge port into the liquid pool of the mixing box; The tissue fluid then enters the hydrolysis chamber through the liquid inlet of the hydrolysis tube, so that the tissue fluid fills the hydrolysis chamber; Then, the connecting rod and the hydrolysis tube connected to the connecting rod are driven downward by the lifting cylinder, so that the liquid inlet of the hydrolysis tube is hidden in the lifting chamber for sealing, and the liquid outlet of the hydrolysis tube is connected to the nozzle of the enzyme reagent container. At this time, the liquid pump of the enzyme reagent container is started to send the enzyme reagent from the nozzle into the hydrolysis chamber for mixing and hydrolysis; When the enzyme reagent enters the hydrolysis chamber, the pressure plate on the hydrolysis chamber is pressed upward, so that the tissue fluid in the hydrolysis chamber enters the avoidance chamber, providing a holding space for the enzyme reagent; When the enzyme reagent and tissue fluid are mixed and hydrolyzed into a solution, the hydrolysis tube is driven downward again by the lifting cylinder, so that the liquid outlet of the hydrolysis tube is connected with the solution outlet of the liquid outlet nozzle, and then the solution in the hydrolysis chamber is discharged from the liquid outlet nozzle; Finally, the absorbance of the solution discharged from the outlet nozzle is detected by spectral technology to obtain the drug residue concentration of the raw material.

9. The drug residue detection method according to claim 8, characterized in that: In the above steps, during the process of discharging the solution, the pressing plate is reset under the action of its own gravity as the liquid level in the hydrolysis chamber drops; When the solution in the hydrolysis chamber is completely discharged, the hydrolysis tube is driven to reset by the lifting cylinder so that the liquid inlet of the hydrolysis tube is connected to the liquid pool again.

10. The drug residue detection method according to claim 8, characterized in that: In the above steps, after the enzyme reagent enters the hydrolysis chamber, ultrasonic waves are emitted by the ultrasonic generator on the pressing plate, so that the enzyme reagent and the tissue fluid produce a cavitation effect when mixed, thereby promoting the mixing of the enzyme reagent and the tissue fluid.

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