Saliva detection pen and use method thereof

Abstract

The invention provides a saliva detection pen and a use method thereof. The detection pen comprises a circuit device, a liquid absorption rod, a test strip and a control chip, the circuit device comprises a circuit line and an indicator lamp, and the liquid suction rod comprises a liquid suction end and a conveying end; the circuit line is connected with the conveying end of the liquid absorption rod, the indicator lamp and the control chip. When a to-be-detected sample flows and migrates to the conveying end from the liquid absorbing end of the liquid absorbing rod, the circuit is connected, and the indicating lamp prompts in a flickering or normally-on mode; and the control chip is used for controlling the reaction time. According to the saliva detection pen provided by the invention, the saliva detection test strip is combined with the liquid absorption rod, the circuit device and the control chip for use, so that the problem of result errors caused by factors such as inaccurate reading time and dim light is solved.

Description

Technical Field

[0001] The invention belongs to the technical field of rapid detection, and in particular relates to a saliva detection pen and a use method thereof. Background Art

[0002] Saliva is a complex mixture that contains not only various proteins, but also DNA, RNA, fatty acids, and various microorganisms. Studies have found that various protein components in the blood are also present in saliva. Saliva can reflect changes in the levels of various proteins in the blood. Analysis of saliva components can help us understand the health and disease status of the body. Various proteomics, transcriptomics, and microbial markers in saliva have been identified as effective indicators for diagnosing oral and systemic diseases.

[0003] Saliva has been used to test HIV, HBV, and various drugs such as cocaine and alcohol. Research on saliva testing has just started. The saliva testing products used in the existing technology require strict reading time, but because there is no time prompt when the product is used, reading outside the time required by the instructions will lead to different results; at the same time, the product needs to be read by the naked eye, and sometimes the reading will be inaccurate due to differences in the environment when reading the results, such as dim light.

[0004] Patent document CN108051579A discloses a portable AIDS saliva detector, which is provided with a circuit board and a housing. The circuit board includes multiple structures such as an indicator light, a buzzer, and a temperature sensor. When in use, the outer shell cap needs to be unscrewed, and the saliva is placed in the outer shell cap with a diluent and stirred and mixed. Then, the inner shell cap is unscrewed and the outer shell cap containing the liquid is screwed to the housing of the detector. At this time, the AIDS saliva test paper automatically absorbs the test liquid to start the test. The device needs to go through multiple operation steps such as sampling, mixing, and testing, and the reaction time requires the user to start the countdown by pressing a button. The operation process is complicated, and the reading result may produce a reaction time error due to improper human operation.

[0005] Therefore, there is an urgent need to provide a saliva detection device that can automatically complete sampling, detection, timing, and has functions such as prompting and lighting, so as to further improve the accuracy and sensitivity of saliva detection. Summary of the invention

[0006] The present invention provides a saliva detection pen and a method for using the same. The detection pen includes a circuit device, a liquid suction rod, a test strip and a control chip, wherein the circuit device includes a circuit line and an indicator light, and the liquid suction rod includes a liquid suction end and a delivery end, and the delivery end is simultaneously connected to the positive and negative electrodes of the circuit line and the test strip. During the detection process, the sample migrates from the liquid suction end to the delivery end, the circuit is connected, and the indicator light is on to indicate that the sample is sufficiently collected and the reaction begins. The saliva detection pen solves the problems of inaccurate reading time and insufficient light, and improves the accuracy of saliva detection.

[0007] In the prior art, most of the sampling and testing integrated detection devices, such as early pregnancy test pens, fecal occult blood test tubes, saliva drug detection pens, urine drug detection cups, etc., reduce the operation steps, facilitate the operation of end customers and obtain more accurate results. However, due to insufficient sampling (especially saliva products), or not strictly following the reading time, there are no clear prompts and other factors that lead to abnormal or biased results, and because of the large difference in reading environment, the reading results are incorrect. For example, drug detection is usually carried out at night, and the police provide detection pens to detect suspected objects. Because the light is dim and time is often tight, it is necessary for the saliva detection pen to have a more obvious light indication during the detection process, and to remind the detection pen of the detection process through the light, and complete the detection as quickly as possible to give the final test results.

[0008] The present invention designs a circuit device, a liquid-absorbing rod, a test strip and a control chip according to the characteristics of existing immunochromatography products. The indicator light in the circuit device can prompt the start of the reaction, and the positive and negative electrodes are set at the delivery end of the liquid-absorbing rod. After the liquid absorption is completed, the positive and negative electrodes are connected, and the indicator light flashing process starts. The control chip presets the reaction time of the indicator light flashing, which is generally 4 minutes. When the reaction time reaches 4 minutes, the indicator light is long on, indicating that the reading can be read.

[0009] However, in actual use, it is often found that due to the influence of factors such as the material and pore size of the pipette rod, the sample flow rate in the pipette rod may be inconsistent, causing some samples to reach the test strip and start to react, while some samples are still in the pipette rod and do not have time to react. As a result, when the 4-minute reaction time is reached, the sample has not had time to complete the reaction, and the test result is negative or weakly positive. Of course, this problem can also be solved by extending the reaction time during the test, but this will bring more time costs for the tester and the testee to wait together, and extending the reaction time cannot solve the problems caused by the test equipment itself.

[0010] Therefore, it is necessary to determine key factors such as the material of the pipette rod, electrode material, electrode distance, etc., accurately control the flow consistency of the sample in the pipette rod, and start the reaction when it reaches the test strip at the same time. The positive and negative electrodes also need to sensitively react to the process of connecting the circuit after the sample reaches the pipette rod, thereby improving the sensitivity and accuracy of the test results.

[0011] On the one hand, the present invention provides a saliva detection pen, including a circuit device, a liquid absorption stick, and a test strip, wherein the circuit device includes a circuit line and an indicator light.

[0012] In the existing saliva detection technology, generally only saliva test strips or detection pens with absorbents and test strips are used for saliva sampling detection and analysis, wherein one end of the absorbent of the test pen is connected to the test strip. The absence of clear prompts leads to abnormal or biased results, and the reading results are incorrect due to large differences in the reading environment. In the detection pen provided by the present invention, the delivery end of the absorbent rod is connected to the circuit device and the test strip at the same time. When the test sample moves from the absorbent end of the absorbent rod to the delivery end, the circuit is connected and the indicator light starts to light up; in addition, because there is a partial overlap between the bottom of the test strip and the delivery end of the absorbent rod, the sample enters the test strip at the same time, and the reaction starts when the indicator light turns on. Compared with the prior art, an indicator light device is added to mark the detection process.

[0013] Furthermore, the liquid aspiration rod includes a liquid aspiration end and a delivery end, the liquid aspiration end is used to contact and absorb the sample; the delivery end is used to transfer the sample to the test strip; the delivery end is connected to the positive and negative electrodes of the circuit line.

[0014] The liquid-absorbing rod provided by the present invention includes a liquid-absorbing end and a conveying end. When the sample migrates from the liquid-absorbing end to the conveying end, the indicator light starts to flash when the circuit is connected, indicating that the sample is fully collected, so that the sample enters the test strip to participate in the detection and analysis after the collection is stopped, and the data result is reliable and accurate. In this collection and detection process, the indicator light prompts that the sample collection is completed and the sampling can be ended to enter the analysis. The subsequent reaction process is carried out by controlling the reaction time set by the chip, thereby improving the accuracy of the test result. In addition, the liquid-absorbing rod provided by the present invention adopts biocompatible materials and is disinfected and sterilized. Compared with the method of dipping the saliva sample and then analyzing it on the test strip in the prior art, the liquid-absorbing rod can be directly contained in the mouth to absorb the saliva sample, which simplifies the sample collection and analysis steps, is more convenient in actual use, and is simpler to operate.

[0015] Furthermore, the liquid-absorbing rod is a porous fiber; the material of the porous fiber includes any one or more of polyolefin, polyester, and polyamide, and the pore size is 5 to 10 μm.

[0016] In some embodiments, the liquid-absorbing rod provided by the present invention is a porous fiber, and the test strip material is a nitrocellulose membrane.

[0017] The chemical properties of porous fibers are determined by their materials. Choosing the right polymer material is very important to ensure the long-lasting function of the saliva test pen and the accuracy of the test results. The material of the porous fiber is usually selected based on the chemical substances it contacts. The present invention compares and analyzes the accuracy of the results when several different porous fiber materials such as polyolefins, polyesters, and polyamides are used to collect and analyze fat-soluble drugs such as morphine. It is found that polyolefin materials have good water absorption capacity and high sensitivity compared with other materials; while other materials will cause uneven sample adsorption and inaccurate results when tested.

[0018] At the same time, the pore size of porous fibers can be large or small depending on the material, which will directly affect the resolution of the analysis, the sample flow rate, and the sensitivity of the test. By selecting porous fibers with appropriate pore sizes, the aspiration speed and filtration efficiency of the aspirator can be better controlled. Therefore, the porous fibers with different pore sizes were further screened using morphine standards, and the results showed that the pore size of the porous fiber suitable for morphine drug detection was 10μm.

[0019] Furthermore, the electrode materials of the positive and negative electrodes include any one or more of gold, platinum, copper and iron.

[0020] Since the connection of the circuit device starts when the sample liquid migrates to the delivery end and a chemical reaction occurs, it is necessary to select a suitable electrode material to improve the reaction sensitivity. Electrode materials suitable for chemical reactions need to consider multiple factors, such as chemical stability, electrochemical activity, and electrical conductivity. Through screening experiments, the present invention found that the metal electrode material platinum can provide significantly improved sensitivity and accuracy of reaction detection due to its excellent electrochemical activity, chemical stability, and electrical conductivity.

[0021] Furthermore, the distance between the positive and negative electrodes is 5 to 10 mm.

[0022] When the sample is fully collected and migrated to the delivery end, the circuit device is connected and the indicator light starts to light up. In the previous process, the migration speed and adsorption amount of the sample on the pipette stick are particularly critical. In addition to the material and pore size of the pipette stick that affect the sample adsorption amount, the distance between the positive and negative electrodes at the delivery end of the pipette stick (i.e., the electrode distance) will also affect the final test results. If the electrode distance is too large, it will result in the need to collect excessive samples to connect the circuit, and the indicator light flashing time will lag behind the reaction time, resulting in insufficient reaction; if the electrode distance is too small, it will result in insufficient sample collection, and the reaction will start prematurely. The test result may be misdetected from a positive result to a weak positive or negative result.

[0023] In some embodiments, the distance between the positive and negative electrodes is 5 mm.

[0024] Furthermore, the detection pen also includes a control chip and a shell, the control chip is located on one side of the liquid-absorbing rod; the shell includes a shell and a pen cap.

[0025] The saliva detection pen provided by the present invention is also provided with a control chip, which can set the reaction time. When the indicator light indicates the start of the reaction, the chip starts the countdown until the end of the reaction, thereby avoiding reading errors caused by manual timing or naked eye observation.

[0026] In some embodiments, the control chip sets the reaction time to 4 minutes.

[0027] In some embodiments, a housing and a pen cap are provided on the circuit device, the liquid-absorbing rod, the test paper strip and the control chip, and the housing includes a first housing and a second housing, and an observation window is provided on the first housing for observing and recording the result. The pen cap is used to protect the liquid-absorbing end of the liquid-absorbing rod, and a diaphragm is installed in the pen cap. When the pen cap is removed, the control chip controls the indicator light to flash once, indicating that the detection pen is in normal state.

[0028] On the other hand, the present invention provides a method for saliva detection, characterized in that the detection is performed using the saliva detection pen as described above.

[0029] Furthermore, the method comprises the following steps:

[0030] (1) Remove the pen cap and the indicator light will light up to indicate that the test pen is in a valid state and can be used;

[0031] (2) The aspiration end of the aspiration stick absorbs the sample, the liquid migrates and flows to the delivery end, the circuit line is connected, the indicator light turns on to indicate that the sample is sufficiently collected, and sampling stops;

[0032] (4) The indicator light remains on, indicating that the reaction is complete and the test is completed. The result is recorded.

[0033] Furthermore, in steps (1) and (2), the indicator light is lit in a flashing manner.

[0034] In some methods, when the pen cap is removed, the indicator light will flash once to indicate a normal state. If the indicator light is abnormal, the test pen needs to be checked. When the sample liquid reaches the delivery end, the circuit is connected, and the indicator light begins to flash continuously to indicate the start of the reaction. When the reaction ends, the indicator light stops flashing and remains on, providing a certain amount of light for recording the results, thereby improving the accuracy of the readings.

[0035] On the other hand, the present invention provides a use of a detection pen for preparing a device for improving the accuracy of saliva detection, wherein the device includes a circuit device, a liquid-absorbing rod, a test strip, a control chip and a housing; the circuit device includes a circuit line and an indicator light.

[0036] The detection pen provided by the present invention is provided with an indicator light and a control chip, which can accurately control the reaction time and provide lighting light for result reading; at the same time, the material, aperture, electrode material, etc. of the liquid-absorbing rod are screened, and the most suitable device for reaction detection is provided according to the needs of the detection purpose, thereby improving the accuracy and sensitivity of saliva detection and analysis.

[0037] Compared with the prior art, the present invention has the following advantages:

[0038] 1. The saliva detection pen provided by the present invention is provided with an indicator light, which can clearly indicate the reaction process and improve the accuracy of readings in dim environments;

[0039] 2. The control chip can control the detection operation process and set the reaction time; the porous fiber is used as the material of the morphine drug absorption rod, and metal platinum is used as the electrode material, which accurately controls the sample flow rate and the sample amount required for subsequent reactions, thereby improving the accuracy and sensitivity of the detection;

[0040] 3. The present invention is easy to use and improves the effectiveness and application scope of morphine drug detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of the appearance structure of the saliva detection pen in Example 1;

[0042] Figure 2 This is a schematic diagram of the disassembled structure of the saliva detection pen in Example 1;

[0043] Figure 3 This is a schematic diagram of the cross-sectional structure of the saliva detection pen in Example 1. DETAILED DESCRIPTION

[0044] The preferred embodiments of the present invention are further described in detail below in conjunction with the accompanying drawings. It should be noted that the embodiments described below are intended to facilitate the understanding of the present invention and do not have any limiting effect on it. The raw materials and equipment used in the specific embodiments of the present invention are all known products and are obtained by purchasing commercially available products.

[0045] The following are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0046] Example 1: Saliva detection pen provided by the present invention

[0047] The structure of the saliva detection pen provided in this embodiment is as follows Figures 1 to 3 As shown, Figure 1 This is a schematic diagram of the appearance and structure of a saliva testing pen; Figure 2 This is a schematic diagram of the disassembled structure of the saliva detection pen; Figure 3This is a schematic diagram of the cross-sectional structure of a saliva testing pen.

[0048] like Figures 1 to 3 As shown, the saliva detection pen 1 provided by the present invention includes a circuit device 2, a liquid absorption stick 3, a test strip 4, a control chip 5 and a shell 6, wherein the shell 6 includes a shell 61 and a pen cap 62, the shell 61 includes a first shell 611 and a second shell 612, and the first shell 611 is provided with an observation window 6111.

[0049] The circuit device 2 includes a circuit line 21 and an indicator light 22. The two ends of the liquid-absorbing rod 31 are respectively a liquid-absorbing end 311 and a delivery end 312. The liquid-absorbing rod 31 is a porous fiber made of polyolefin with a pore size of 10 μm. The two ends of the delivery end 312 of the liquid-absorbing rod 31 are respectively connected to the positive electrode 211 and the negative electrode 212 of the circuit line 21. The two electrode materials are metal platinum, and the electrode distance is 5 mm. The indicator light 22 and the control chip 5 are connected to the circuit device 2 through the circuit line 21, and the control chip 5 is located on one side of the liquid-absorbing rod 3. The inner side of the pen cap 62 is covered with a diaphragm 621, and the control chip 5 senses the control indicator light through the diaphragm 621 to display the detection pen status.

[0050] Example 2: Method of using the saliva detection pen

[0051] The saliva detection pen provided in Example 1 is used to collect, detect and analyze drugs in saliva samples. The specific operation steps are as follows:

[0052] (1) Remove the pen cap and the indicator light will flash, indicating that the test pen is in a valid state and can be used for saliva testing and analysis;

[0053] (2) The aspiration end of the aspiration rod collects the sample, and the liquid flows and migrates to the delivery end of the aspiration rod. The liquid connects the circuit, and the indicator light starts to flash continuously, indicating that the sample is collected sufficiently and the reaction begins. The reaction time is 4 minutes, and the sampling stops;

[0054] (3) The indicator light stops flashing and remains on, indicating that the reaction is over and the test is completed;

[0055] (4) Record the test results on the test strip through the observation window.

[0056] Example 2: Material screening of porous fibers

[0057] The porous fibers used in the prior art generally include polyolefins, polyesters, polyamides, and biodegradable materials such as cellulose acetate. Porous fibers of different materials have different chemical properties, so there are differences in drug absorption capacity and hydrophilicity in saliva samples. For the adsorption of saliva samples, especially the adsorption materials that can improve the accuracy and sensitivity of morphine drug detection, this embodiment uses the three porous fiber materials shown in Table 1 to prepare a saliva detection pen. The other structures are the same as those in Example 1. According to the detection method described in Example 2, 200μl morphine standard samples with concentrations of 0ng / ml, 100ng / ml, 200ng / ml, and 300ng / ml are used for detection and analysis. Three replicates are set for each concentration to screen porous fiber materials suitable for morphine drug adsorption detection. The test results are shown in Table 1.

[0058] Table 1 Test results of different material pipette rods

[0059]

[0060] Note: “+” indicates positive, “±” indicates weak positive, and “-” indicates negative.

[0061] According to Table 2, when the saliva test pens prepared by the three materials were used for testing under low concentration conditions (0 ng / ml and 100 ng / ml), the test results of the polyester material were less consistent, with a weak positive result under the 0 ng / ml concentration condition and a negative result under the 100 ng / ml concentration condition; when the test was performed under high concentration conditions (200 ng / ml and 300 ng / ml), the test results of polyester and polyamide both had weak positive results, poor repeatability, and the detection accuracy was between 50% and 100%; this may be because when polyester or polyamide adsorbed morphine standards, the material had poor adsorption of drugs, the flow of biological molecules in the sample was uneven or partially blocked, resulting in insufficient sample volume when the circuit was connected, the indicator light started timing in advance, and the samples that reacted within the subsequent 4 min reaction time did not react fully, resulting in errors in the test results. When the saliva test pen made of polyolefin material detects morphine drugs, its accuracy is 100%, and the repeatability of the test results is consistent regardless of low-concentration samples or high-concentration samples. Therefore, the polyolefin material significantly improves the sensitivity and detection accuracy of the saliva test pen, and the porous fiber absorbent stick made of polyolefin material is preferred.

[0062] Example 3: Pore size screening of porous fibers

[0063] The porous fiber is used as an adsorption carrier for saliva samples, and the selection of its pore size directly affects the flow rate of the test sample and the sensitivity of the analysis. A larger pore size may make it easier for these biomacromolecules to pass through, but it may result in an excessive amount of sample for reaction and insufficient sample reaction within a limited time; while a smaller pore size can improve the specificity of binding, but it may limit the passage of the analyte, resulting in insufficient sample for subsequent reactions.

[0064] In order to screen the pore size of porous fibers suitable for the collection, detection and analysis of morphine drugs in saliva samples, this embodiment uses 200 μl of morphine with concentrations of 0 ng / ml, 100 ng / ml, 300 ng / ml and 500 ng / ml as a standard, and uses the saliva detection pen provided in Example 1, wherein the pore size of the porous fiber is shown in Table 2. The detection is performed according to the method of use described in Example 2, and the detection results are shown in Table 2.

[0065] Table 2 Test results of porous fibers with different pore sizes

[0066]

[0067] Note: “+” indicates positive, “±” indicates weak positive, and “-” indicates negative.

[0068] As can be seen from Table 2, when the saliva detection pen prepared by the porous fiber with a pore size of 5 μm is used for morphine drug detection, the accuracy is between 25% and 50%, and when the concentration of the standard is higher, the repeatability of the detection result is worse, and the accuracy is lower. It may be due to the small pore size of the porous fiber, which leads to the blockage of the substance in the standard, and the higher the concentration of the standard, the more substances it contains, causing more serious blockage, resulting in inaccurate detection results; when the pore size of the porous fiber is greater than or equal to 7 μm, the repeatability and accuracy of the detection results are improved, wherein when the pore size is equal to 10 μm, the detection result accuracy is 100%, and the repeatability is good; and when the pore size is 12 μm, the biomolecule flow rate in morphine may be too fast, and there are too many samples for reaction, resulting in insufficient reaction, and the detection result is weakly positive. The above results show that a membrane pore size that is too small may cause blockage or uneven liquid flow; too large a pore size may cause too many samples for reaction, resulting in insufficient reaction, therefore, the present embodiment preferably uses a porous fiber with a pore size of 10 μm.

[0069] Example 4: Screening of electrode materials

[0070] Electrode materials suitable for chemical reactions need to consider multiple factors such as chemical stability, electrochemical activity and conductivity. Different electrode materials may cause unstable contact and insensitive sensing in circuit devices due to different materials, which may cause deviations or abnormalities in the response time of the indicator light, further making the detection results unreliable.

[0071] Therefore, in order to screen electrode materials suitable for both ends of the circuit line to improve the sensitivity of the saliva detection process and the accuracy of the results, this embodiment provides saliva detection pens prepared with 4 kinds of electrode materials as shown in Table 3. The other structures are the same as those in Example 1. According to the detection method described in Example 2, 20 portions of morphine standards with different concentrations (100ng / ml to 300ng / ml) are analyzed with a dosage of 200μl per portion, and the detection results and the time when the indicator light starts flashing are recorded. The results are shown in the following table.

[0072] Table 3 Detection of different positive and negative electrode materials

[0073]

[0074]

[0075] Note: “+” indicates positive, “±” indicates weak positive, and “-” indicates negative.

[0076] According to the results in Table 3, when different metals are used as electrode materials, there are differences in the sensitivity of the circuit device and the sampling detection results of the detection pen. When iron and gold are used as electrode materials, the indicator light starts flashing with a time lag, and the detection result accuracy is 70-75%. This may be due to the poor conductivity of these two metal materials in the morphine drug solution. When the sample flow reaches the delivery end and starts to react, the circuit device is not connected in time, and the indicator light does not indicate the start of the reaction, resulting in excessive sample adsorption at the liquid suction end, insufficient subsequent reaction, and weak positive or negative detection results; when copper is used as the metal material, the detection accuracy is 85%, the indicator light flashes in advance, and the detection result is weakly positive. The possible reason is that when copper is used as an electrode material in the morphine solution, its electrochemical activity is poor, and the connection circuit device is not stable enough, resulting in inaccurate indicator light response time and deviation in the detection result; and when platinum sheet is used as the electrode material, the stability of the circuit device is significantly improved, and the detection result accuracy can reach 95%. Therefore, this embodiment preferably uses metal platinum as the electrode material.

[0077] Example 5: Screening of electrode distance

[0078] In order to further analyze whether the interface distance of the electrodes at both ends of the circuit line affects the final detection result, this embodiment uses 200 μl of 20 portions of morphine standards with different concentrations (100 ng / ml to 300 ng / ml) as samples, and uses the saliva detection pen provided in Example 1, in which the electrode distances are set to 2.5 mm, 5 mm, 10 mm, and 12.5 mm. According to the detection method of Example 2, the detection effects of the saliva detection pens under the four electrode distances are compared and analyzed. The results are shown in Table 4.

[0079] Table 4 Effect of different electrode distances on the detection results of morphine standards

[0080]

[0081]

[0082] Note: “+” indicates positive, “±” indicates weak positive, and “-” indicates negative.

[0083] As shown in Table 4, when the electrode distance is 5mm, morphine standards of different concentrations can be accurately detected, and the indicator light flashes normally; when the electrode distance is equal to 7.5mm or 10mm, the detection of morphine standards is reduced to 85%, and the indicator light flashes late, which may be due to the excessive electrode distance, resulting in too many samples required for adsorption of the connected circuit, insufficient subsequent reaction, and inaccurate results; when the electrode distance is 2.5mm, the test results are also inaccurate, with an accuracy rate of 80%, and negative results may be due to the electrode distance being too small. When a small amount of sample flows to the delivery end, the circuit is connected, the indicator light flashes in advance, the reaction starts in advance, and some samples have not yet arrived at the delivery end to start the reaction, resulting in inaccurate results. According to the above analysis, the preferred electrode distance of 5mm in this embodiment.

[0084] The above is only a specific implementation of the present invention, but the design concept of the present invention is not limited thereto. Any non-substantial changes to the present invention using this concept shall be deemed as an infringement of the protection scope of the present invention.

Claims

1. A saliva detection pen, characterized in that: The invention comprises a circuit device, a liquid-absorbing stick and a test paper strip, wherein the circuit device comprises a circuit line and an indicator light.

2. The detection pen according to claim 1, characterized in that: The liquid aspiration rod comprises a liquid aspiration end and a delivery end, wherein the liquid aspiration end is used to contact and absorb the sample; the delivery end is used to transfer the sample to the test strip; and the delivery end is connected to the positive and negative electrodes of the circuit line.

3. The detection pen according to claim 2, characterized in that: The liquid-absorbing rod is a porous fiber; the material of the porous fiber includes any one or more of polyolefin, polyester, and polyamide, and the pore size is 5 to 10 μm.

4. The detection pen according to claim 3, characterized in that: The electrode materials of the positive and negative electrodes include any one or more of gold, platinum, copper and iron.

5. The detection pen according to claim 4, characterized in that: The distance between the positive electrode and the negative electrode is 5 to 10 mm.

6. The detection pen according to claim 5, characterized in that: It also includes a control chip and a shell, wherein the control chip is located at one side of the liquid-absorbing rod; and the shell includes a shell body and a pen cap.

7. A method for saliva detection, characterized in that: The saliva detection pen as described in claims 1-6 is used for detection.

8. The method according to claim 7, characterized in that The following steps are involved: (1) Remove the pen cap and the indicator light will light up to indicate that the test pen is in a valid state and can be used; (2) The aspiration end of the aspiration stick absorbs the sample, the liquid migrates and flows to the delivery end, the circuit line is connected, the indicator light turns on to indicate that the sample is sufficiently collected and the reaction begins, and the sampling stops; (4) The chip controls the reaction time, and the reaction end indicator remains on, indicating that the test is complete and the results are recorded.

9. The method according to claim 8, characterized in that In the steps (1) and (2), the indicator light is lit by flashing.

10. Use of a detection pen for preparing a device for improving the accuracy of saliva detection, characterized in that: The device comprises a circuit device, a liquid-absorbing stick, a test paper strip, a control chip and a shell; the circuit device comprises a circuit line and an indicator light.

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